CN101881735A - Reagent for qualitatively detecting paraquat and method for detecting paraquat in urine - Google Patents
Reagent for qualitatively detecting paraquat and method for detecting paraquat in urine Download PDFInfo
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- CN101881735A CN101881735A CN 201010197563 CN201010197563A CN101881735A CN 101881735 A CN101881735 A CN 101881735A CN 201010197563 CN201010197563 CN 201010197563 CN 201010197563 A CN201010197563 A CN 201010197563A CN 101881735 A CN101881735 A CN 101881735A
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Abstract
The invention relates to a reagent for qualitatively detecting paraquat and a method for detecting the paraquat in urine, which are used for quickly detecting the most common pesticide poisoning, namely paraquat poisoning. The reagent comprises a reagent A and a reagent B, wherein the reagent A is sodium bicarbonate; and the reagent B is alkali sodium dithionite or sodium dithionite. The method for detecting the paraquat comprises the following steps of: taking 10ml of urine of a patient, putting the urine in a clean plastic cup, adding 2.0g of the reagent A into the cup, and after the reagent A is completely and uniformly dissolved, adding 1.0g of the reagent B into the cup, wherein if the urine contains paraquat ions, the urine immediately turns blue, and the darker the blue color is, the higher the concentration of the paraquat in the urine is. The reagent has the advantages of high accuracy, no false positive and false negative, high sensitivity, specificity and uniqueness.
Description
Technical field
This patent relates to a kind of detection method that is used for the reagent and the urine paraquat of qualitative detection paraquat, is used for a kind of modal pesticide poisoning--the quick diagnosis of paraquat poisoning.
Background technology
Paraquat is a kind of herbicide, and agriculture and forestry are widely used, produce with life in contact accidentally and can cause poisoning.This kind poisoning is one of higher poisoning of the present incidence of disease, and diagnosis sometimes is difficulty, the especially indefinite concealment formula of contact history poisoning patient comparatively.Although the patient's contact history that has is clear and definite, the contact amount how much, and the degree of intoxication depth also often is difficult to clearly judge.At present " the blood paraquat detection technique " used clinically though can qualitative, quantitative, technology is very complicated, detection time is longer, and expense is higher.So a kind of quick, easy, accurate, cheap detection method of urgent clinical needs.
Summary of the invention
The present invention provides a kind of detection method that is used for the reagent and the urine paraquat of qualitative detection paraquat in order to overcome the deficiency of above technical elements, to solve the quick diagnosis problem of paraquat poisoning.
Technical scheme of the present invention realizes by following measure:
The invention discloses a kind of reagent that is used for the qualitative detection paraquat, include reagent A and reagent B, described reagent A is: sodium bicarbonate, described reagent B is: alkaline sodium hydrosulfite.
The invention also discloses the detection method of paraquat in a kind of urine, adopt following steps: get the patient and urinate 10ml, place a pure plastic cup, at first add reagent A 2.0g, after treating abundant mixing dissolving, add reagent B1.0g, as contain in the urine paraquat ion then urine be blue immediately, paraquat concentration is higher in the darker explanation urine of blue degree.
Reaction principle: paraquat (paraquat) has another name called Aerial gramoxone, the no track of gram, and chemical name is 1,1 '-dimethyl-4,4 '-bipyridine cation or its salt.
As the quick-acting natural disposition contact killing type herbicide widespread uses of going out, former medicine is white or yellow solid to paraquat, and was soluble in water from beginning in 1962, and its commodity are 20% blue aqueous solution.
Paraquat can absorb by mucocutaneous, intestines and stomach and respiratory tract, and infiltration rate is very fast, and paraquat is absorbed into has behind the human body 90% to be discharged by urine with prototype in 48 hours, still can measure after reaching for 3 weeks.Therefore, all contain the paraquat ion in the urine of paraquat poisoning patient in 3 weeks.
Get patient's urine 10ml, the purpose that places pure plastic tube to add reagent A is an alkalized urine, makes subsequent reactions more stable more obvious.Candidate agent A fully promptly adds reagent B after the reaction in urine, paraquat is reduced to blue free radical under the effect of reagent B, and urine is blue immediately and changes, and blue degree is darker, then contains the paraquat ion the more in the urine.
The beneficial effect of this patent is: this detection method accuracy height, exhausted non-false positive and false negative, highly sensitive, even having micro-paraquat to take in human body can detect, this detection has specificity and uniqueness, have only paraquat ion and this reagent in conjunction with this reaction just occurring, and the tool stable high characteristics that are quick on the draw.
Embodiment:
Example 1
Be the patient of paraquat poisoning through the blood detection, the concentration of paraquat is in its blood: 5.6 micrograms/ml.
Get above-mentioned patient's urine 10ml, place a pure plastic cup, at first add reagent A 2.0g, treat the dissolving of abundant mixing after, add reagent B1.0g, urine is mazarine immediately, illustrate urinate in paraquat concentration higher.
Above-mentioned urine is accurately measured through chemical method, and the concentration of paraquat is in the urine: 50 micrograms/ml.
Example 2
Be the patient of paraquat poisoning through the blood detection, the concentration of paraquat is in its blood: 1.5 micrograms/ml.
Get above-mentioned patient's urine 10ml, place a pure plastic cup, at first add reagent A 2.0g, treat the dissolving of abundant mixing after, add reagent B1.0g, it is light blue that urine is immediately, illustrate urinate in paraquat concentration lower.
Above-mentioned urine is accurately measured through chemical method, and the concentration of paraquat is in the urine: 12 micrograms/ml.
Example 3
Be the patient of paraquat poisoning through the blood detection, the concentration of paraquat is in its blood: 0.4 microgram/ml.
Get above-mentioned patient's urine 10ml, place a pure plastic cup, at first add reagent A 2.0g, treat the dissolving of abundant mixing after, add reagent B1.0g, urine is little blueness immediately, illustrate urinate in paraquat concentration very low.
Above-mentioned urine is accurately measured through chemical method, and the concentration of paraquat is in the urine: 3 micrograms/ml.
Example 4
Detect the patient of the some poisonings that are glyphosate, organophosphorus, cinerins through blood.
Get above-mentioned patient's urine 10ml, place a pure plastic cup, at first add reagent A 2.0g, treat the dissolving of abundant mixing after, add reagent B1.0g, the urine color is not variation all.
Example 5
Detect some normal persons through blood, in contrast.
Get above-mentioned normal person's urine 10ml, place a pure plastic cup, at first add reagent A 2.0g, treat the dissolving of abundant mixing after, add reagent B1.0g, the urine color is not variation all.
Adopt detection method of the present invention, compare with blood testing, as following table:
| Blood testing | Urine detection | |
| The biological specimen pre-service | Need | Do not need |
| Requirement to checkout equipment | Need high-end checkout equipment as gas chromatograph/mass spectrometer or high performance liquid chromatogram instrument | Need not equipment |
| Detection time | Need more than 24 hours approximately | Need 3 minutes |
| Testing cost | Need hundreds of units approximately | Need dozens of yuan |
| Testing result | Accurately, can be qualitative, quantitative | Accurately, can be qualitative |
Claims (2)
1. a reagent that is used for the qualitative detection paraquat is characterized in that including reagent A and reagent B, and described reagent A is a sodium bicarbonate, and described reagent B is alkaline sodium hydrosulfite or sodium hydrosulfite.
2. the detection method of paraquat in the urine, it is characterized in that adopting following steps: get the patient and urinate 10ml, place a pure plastic cup, at first add reagent A 2.0g, after treating abundant mixing dissolving, add reagent B1.0g, as contain in the urine paraquat ion then urine be blue immediately, paraquat concentration is higher in the darker explanation urine of blue degree.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101975636A CN101881735B (en) | 2010-06-11 | 2010-06-11 | Method for detecting paraquat in urine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010101975636A CN101881735B (en) | 2010-06-11 | 2010-06-11 | Method for detecting paraquat in urine |
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| CN101881735A true CN101881735A (en) | 2010-11-10 |
| CN101881735B CN101881735B (en) | 2011-11-16 |
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| CN2010101975636A Expired - Fee Related CN101881735B (en) | 2010-06-11 | 2010-06-11 | Method for detecting paraquat in urine |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102053069A (en) * | 2010-12-01 | 2011-05-11 | 新乡医学院 | Kit for rapid determination of paraquat concentration in blood |
| CN103952149A (en) * | 2014-05-21 | 2014-07-30 | 齐齐哈尔大学 | Fluorescent detection reagent and detection method for dichlorinated-1, 1'-dimethyl-4, 4'-dipyridyl |
| CN104607259A (en) * | 2015-01-22 | 2015-05-13 | 中国人民武装警察部队后勤学院附属医院 | Paraquat detection reagent tube |
| CN110220896A (en) * | 2019-07-09 | 2019-09-10 | 南通大学附属医院 | A kind of qualitative half-quantitative detection kit of poisonous substance and the qualitative semi-quantitative method of poisonous substance |
| CN111781199A (en) * | 2020-07-17 | 2020-10-16 | 吉林大学 | Method for rapidly detecting paraquat and diquat in urine |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5968746A (en) * | 1997-11-26 | 1999-10-19 | Schneider; David R. | Method and apparatus for preserving human saliva for testing |
| CN101324575A (en) * | 2008-07-10 | 2008-12-17 | 江南大学 | Preparation and application of paraquat semi-quantitative rapid test reagent strip |
| JP5042626B2 (en) * | 2003-09-22 | 2012-10-03 | エス＊バイオ プライベート リミティッド | Benzimidazole derivatives: production and pharmaceutical applications |
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2010
- 2010-06-11 CN CN2010101975636A patent/CN101881735B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5968746A (en) * | 1997-11-26 | 1999-10-19 | Schneider; David R. | Method and apparatus for preserving human saliva for testing |
| JP5042626B2 (en) * | 2003-09-22 | 2012-10-03 | エス＊バイオ プライベート リミティッド | Benzimidazole derivatives: production and pharmaceutical applications |
| CN101324575A (en) * | 2008-07-10 | 2008-12-17 | 江南大学 | Preparation and application of paraquat semi-quantitative rapid test reagent strip |
Non-Patent Citations (1)
| Title |
|---|
| 《中国卫生检验杂志》 20080531 孙世义，等 分光光度法快速测定尿中百草枯 819，874 1 第18卷, 第5期 2 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102053069A (en) * | 2010-12-01 | 2011-05-11 | 新乡医学院 | Kit for rapid determination of paraquat concentration in blood |
| CN102053069B (en) * | 2010-12-01 | 2012-05-02 | 新乡医学院 | Kit for rapid determination of paraquat concentration in blood |
| CN103952149A (en) * | 2014-05-21 | 2014-07-30 | 齐齐哈尔大学 | Fluorescent detection reagent and detection method for dichlorinated-1, 1'-dimethyl-4, 4'-dipyridyl |
| CN104607259A (en) * | 2015-01-22 | 2015-05-13 | 中国人民武装警察部队后勤学院附属医院 | Paraquat detection reagent tube |
| CN110220896A (en) * | 2019-07-09 | 2019-09-10 | 南通大学附属医院 | A kind of qualitative half-quantitative detection kit of poisonous substance and the qualitative semi-quantitative method of poisonous substance |
| CN111781199A (en) * | 2020-07-17 | 2020-10-16 | 吉林大学 | Method for rapidly detecting paraquat and diquat in urine |
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| Publication number | Publication date |
|---|---|
| CN101881735B (en) | 2011-11-16 |
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