CN101617959B - External connection fixing device for manufacturing rat spine subluxation animal model - Google Patents
External connection fixing device for manufacturing rat spine subluxation animal model Download PDFInfo
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- CN101617959B CN101617959B CN2008100435953A CN200810043595A CN101617959B CN 101617959 B CN101617959 B CN 101617959B CN 2008100435953 A CN2008100435953 A CN 2008100435953A CN 200810043595 A CN200810043595 A CN 200810043595A CN 101617959 B CN101617959 B CN 101617959B
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Abstract
The invention discloses an external connection fixing device for manufacturing a rat spine subluxation animal model. In the device, the oblique part of a first pair of spinal steel plates is clamped and fixed with the fourth lumbar spinal segment of the rat; the oblique part of a second pair of spinal steel plates is clamped and fixed with the fifth lumbar spinal segment of the rat; the oblique part of a third pair of spinal steel plates is clamped and fixed with the sixth lumbar spinal segment of the rat; and straight parts of the spinal steel plates are fixed and connected together by bolts and nuts through a connecting steel plate. The device can be used for manufacturing a fixing model for preparing L4-L6 segments from the fourth to the sixth lumbar spine of the rat, and can objectively and comprehensively reflect the biomechanical characteristics of a living spine (after the spine is fixed).
Description
Technical field
The present invention relates to a kind of device that is used to make animal model, particularly relate to a kind of outside that makes rat vertebral subluxation animal model and connect fixture.
Background technology
Spinal column is sick and wounded to be the human common slight illness of puzzlement, and traditional Chinese medical science utilization spinal column manipulation of massage has long history and abundant clinical practice accumulation.Study of evidence based medicine shows that the spinal column manipulation of massage has the operating technology maturation, curative effect is sure, relapse rate is low, the high tangible clinical advantage of potency ratio, is a kind of safe and effective procedure.Spinal column is massaged has become the first-selected physiotherapy of recommending use in the lumbago clinical practice guideline.
The traditional Chinese medical science is used term description spine disorderses such as " the vertebra fissure of displacement ", " dislocation ", " displacement " always; West maneuver medical science is referred to as " inferior dislocation " (subluxation); For a long time because the iconography lack of evidence that the fine anatomical position in joint changes; Relevant with it signs of disease can not get enough anatomy supports, and therefore inferior dislocation also becomes the focus of academia long-running argument.Along with deepening continuously of spine structure pathological study, think that at present the spinal column manipulation of massage through the adjustment to the motion segment spatial sequence, can improve the interior environment of nerve, blood vessel, thus blocking-up disease pathology endless-chain.1997, ACC (world is by ridge community) was defined as a kind of " the pathologic joint changes syndrome " with the inferior dislocation of vertebral body.WHO was defined as the Asia dislocation " a kind of joint of structural integrity or motion segment damage or the dysfunction on sequence, motion integrity and/or physiological function; this damage or dysfunction possibly influence biomechanics and neural integrity " at " the WHO guidelines on basic training and safety inchiropractic " of its issue in 2005; The notion of inferior dislocation has contained traditional method medical science said " subluxation ", and its understanding rises to the unification of function pathology and structure pathology from the structure pathological change.
Spinal column is the biomethanics structure of a complicacy, too relies on structure pathology to be prone to thinking limitation is explained category at the modern medicine of machinery.Discover that the backache above 90% is because due to joint and the muscular tissue mechanics problem, function pathology (mechanics problem of articular muscle) changes lumbago caused more more common than structure pathology (fracture, intervertebral disk hernia and other X line are visible unusual) change.With advancing age; Neural protective capability descends; In the damage and the bad process of posture of bearing a heavy burden for a long time repeatedly, can occur following the joint of vertebral column that range of motion reduces, muscle protection property reaction and inflammatory reaction are caused tetanic fixing, the patient often has the sensation of a certain joint of vertebral column movable " being blocked "; Form joint of vertebral column and limited its active locking (Fixation) state in certain position wholly or in part, the intervention of spinal column manipulation of massage is many can to obtain clinical improvements at once.From this phenomenon, can obtain prompting, when the inferior dislocation of research, the single spatial sequence that will consider spinal motion segment, more should fully pay attention to the sections activity change and with factors such as biomechanics characteristic.
Research of Animal Model for Study is the important method of basic medical research.Because the restriction of medical ethics, human body receives strict restriction in the scale-model investigation of body spinal column, is main with isolated model research still at present.Although obtained a large amount of basic biomechanics data,, therefore set up relevant animal model and just seem very necessary because many phenomenons can not rely on isolated model to obtain.At present domestic many through operating method, destroy the inside and outside steady-state conditions of spinal column, models such as preparation cervical spondylosis, prolapse of lumbar intervertebral disc stress to observe its structure pathological change.But it is bigger that relevant modeling method destroys organizational structure, describes less to spinal column at the biomechanics characteristic of body.
Nineteen sixty-five, Cleveland takes the lead in attempting setting up subluxation animal model with meiofauna.A large amount of researchs show that the inferior dislocation of vertebral body is as the key concept of maneuver medical science, and its intension should comprise that the joint anatomical position changes and joint motion reduces by two aspect contents.Thereafter can be divided into two big types on the modeling method of relevant research basically: a kind of is that the mechanics intervention causes joint dislocation, and a kind of is to cause joint stiffness with the method that reduces joint motion.But because the selection of animal, modeling method and observation index are different, and the stability of model and repeatability remain further perfect, and generally accepted vertebral body subluxation animal model is not arranged at present as yet.Therefore, how improving modeling and detection method, reduce the destruction of organizational structure in experimentation, reflect the biomechanics characteristic of live body spinal column as far as possible comprehensively, objectively, is to be based upon the body animal model, and the inferior dislocation of research vertebral body essence is needed the problem of solution badly.
Summary of the invention
The technical problem that the present invention will solve provides a kind of outside connection fixture that is used to make rat vertebral subluxation animal model, can be used in preparation rat the 4th to the 6th spinous process of lumbar vertebra L
4-L
6The fixed model of sections.
For solving the problems of the technologies described above; The outside connection fixture that is used to make rat vertebral subluxation animal model of the present invention, by three pairs of spinous process steel plates, two connect steel plates and a plurality of screw and nut and form; The material of all parts and screw-nut all adopts orthopaedics to use rustless steel, wherein:
Every pair of spinous process steel plate all has curved oblique portion of portion and this straight portion of edge always to the right; Angle between the straight portion of first pair of spinous process steel plate and the oblique portion is less than the straight portion of second pair of spinous process steel plate and the angle between the oblique portion, and the angle between the straight portion of second pair of spinous process steel plate and the oblique portion is less than the straight portion of the 3rd pair of spinous process steel plate and the angle between the oblique portion; Above-mentioned each to the identical length of the straight portion of spinous process steel plate and oblique portion etc.; Straight portion upper end at every pair of spinous process steel plate is provided with a through hole, and the roughly centre position that is positioned at said straight portion is provided with second through hole, and the oblique portion that is positioned at every pair of spinous process steel plate is provided with third through-hole;
The oblique portion of first pair of spinous process steel plate grips rat fourth lumbar vertebra spinous process sections, and the oblique portion of second pair of spinous process steel plate grips rat fifth lumbar vertebra spinous process sections, and the oblique portion of the 3rd pair of spinous process steel plate grips rat the 6th spinous process of lumbar vertebra sections; First screw and first nut are fixed the oblique portion of every pair of spinous process steel plate with corresponding spinous process sections; The oblique portion of each spinous process steel plate matches with the angle that becomes of spinous process with vertebral body; The straight portion and the horizontal line of each spinous process steel plate are perpendicular;
The straight portion of first pair of spinous process steel plate is connected steel plate with first, and the straight portion of the 3rd pair of spinous process steel plate is connected steel plate with second, all adopts second screw to fixedly connected with second nut; The straight portion of second pair of spinous process steel plate is connected the long through-hole end that steel plate is connected with second on the steel plate fixedlys connected with second nut with the 3rd screw with first, and second connects steel plate and be positioned at first upper end that connects steel plate.
Based on two fundamental characteristics of vertebral body displacement and joint motion minimizing that Asia dislocation is had, adopt the device of the present invention can be with rat the 4th to the 6th spinous process of lumbar vertebra L
4-L
6Sections connects from external stability, defines rat the 4th to the 6th spinous process of lumbar vertebra L
4-L
6The joint motion of sections makes the joint be in the dysfunction state of imagination, thereby prepares rat the 4th to the 6th spinous process of lumbar vertebra L
4-L
6The fixed model of sections; The biomechanics characteristic that can more objective, comprehensively reflect the live body spinal column.
Description of drawings
Below in conjunction with the accompanying drawing and the specific embodiment the present invention is done further detailed explanation:
Fig. 1 is device of the present invention and rat the 4th to the 6th spinous process of lumbar vertebra L
4-L
6The sketch map that sections is fixedly connected, wherein, Fig. 1 (a) is a front view, Fig. 1 (b) is a vertical view;
Fig. 2 is an apparatus structure sketch map of the present invention;
Fig. 3 is first pair of spinous process steel plate structure sketch map among Fig. 2;
Fig. 4 is second pair of spinous process steel plate structure sketch map among Fig. 2;
Fig. 5 is the 3rd pair of spinous process steel plate structure sketch map among Fig. 2;
Fig. 6 is the first connection steel plate structure sketch map among Fig. 2;
Fig. 7 is the second connection steel plate structure sketch map among Fig. 2;
Fig. 8 is the first bolt structure sketch map among Fig. 2;
Fig. 9 is the second bolt structure sketch map among Fig. 2;
Figure 10 is the 3rd a bolt structure sketch map among Fig. 2;
Figure 11～20th, one embodiment of the invention test group, matched group, blank control group implementation process sketch map;
Figure 21 is that one embodiment of the invention are implemented the back to PBA angle under test group, matched group, the blank control group observation back hyperextension state (be rat vertebral body trailing edge angle, constitute along L4 vertebral body trailing edge and the crossing angle that is become of L6 vertebral body straight line that trailing edge is done by two) the box cartogram of data.
The specific embodiment
The structure that the outside that is used to make rat vertebral subluxation animal model of the present invention connects fixture is as shown in Figure 2, and by three pairs of spinous process steel plates, two connect steel plates and a plurality of screw and nut and form.First pair of spinous process steel plate 4 is used to grip rat fourth lumbar vertebra spinous process L
4Sections, second pair of spinous process steel plate 5 is used to grip rat fifth lumbar vertebra spinous process L
5Sections, the 3rd pair of spinous process steel plate 6 is used to grip rat the 6th spinous process of lumbar vertebra L
6Sections.Every pair of spinous process steel plate all has curved oblique portion of portion and this straight portion of edge always to the right.Angle between the straight portion of first pair of spinous process steel plate 4 and the oblique portion is less than the straight portion of second pair of spinous process steel plate 5 and the angle between the oblique portion, and the angle between the straight portion of second pair of spinous process steel plate 5 and the oblique portion is less than the straight portion of the 3rd pair of spinous process steel plate 6 and the angle between the oblique portion.In conjunction with Fig. 3,4, shown in 5, above-mentioned each to the straight portion of spinous process steel plate 4,5,6 and the oblique identical length etc. of portion.Straight portion upper end at every pair of spinous process steel plate is provided with first through hole; The roughly centre position that is positioned at said straight portion is provided with second through hole, and the effect of this through hole makes the spinous process steel plate in same level after being rat spinous process steel plate implantation; The oblique portion that is positioned at every pair of spinous process steel plate is provided with third through-hole.
The said end that first and second connects steel plate 1,3 is provided with a through hole, and the other end is provided with a long through-hole, first connect steel plate 1 through hole and long through-hole centre-to-centre spacing greater than second be connected through hole with the long through-hole of steel plate 3 centre-to-centre spacing (combine Fig. 6, shown in 7).
In conjunction with shown in Figure 1, when adopting the present invention to make rat vertebral subluxation animal model, with the oblique portion of first pair of prominent steel plate 4, second pair of spinous process steel plate 5 and the 3rd pair of spinous process steel plate 6 clamping rat the 4th, the 5th and the 6th spinous process of lumbar vertebra L respectively
4-L
6Sections is fixed every pair of spinous process steel plate and spinous process sections with first screw 9 and first nut 7 then.The oblique portion of spinous process steel plate (this oblique portion is for implanting the intravital part of rat) should match with the become angle of spinous process with vertebral body.The straight portion of spinous process steel plate (this straight portion is the part that is positioned at rats in vitro) should be perpendicular with horizontal line, can link to each other with loading test device.The through hole that the straight portion of first pair of spinous process steel plate 4 and first is connected steel plate 1 adopts second screw 8 to be fixedly connected with second nut 2; The through hole that the straight portion of the 3rd pair of spinous process steel plate 6 and second is connected steel plate 3 adopts second screw 8 to be fixedly connected with second nut 2; The straight portion of second pair of spinous process steel plate 5 is connected steel plate 1,3 with first and second long through-hole adopts the 3rd screw 10 to be fixedly connected with second nut 2, and should make second to connect the upper end that steel plate 3 is positioned at the first connection steel plate 1.On said the 3rd screw 10 packing ring 11 can be set, when being used to prevent that the 3rd screw 10 is screwed into second nut 2, second pair of spinous process steel plate 5 and other two pairs of spinous process steel plates occur not on same straight line, cause the problem of swing offset to occur.
The material of all parts and screw-nut all adopts orthopaedics to use rustless steel in device of the present invention.
According to the experimental design requirement, the described outside fixture that connects should be convenient to fix, according to the 4th to the 6th spinous process of lumbar vertebra L
4, L
5, L
6The difference of sections form, each Element Design specification is difference to some extent.Simultaneously, the vertebral rigidity test will load through the second spinous process steel plate, 5 external ends.
Shown in Fig. 3-5, according to the rat spinous process form anatomical data of measuring, foregoing all spinous process steel plate specifications are following: straight minister's degree is 15mm, and tiltedly minister's degree is 5.5mm, and steel plate launches long 20.5mm, and steel plate width is 4.0mm, and steel plate thickness is 1.2mm.The straight portion of first pair of spinous process steel plate 4 and oblique portion angle are 138 ± 0.5 ° (referring to Fig. 3).The straight portion of second pair of spinous process steel plate 5 and oblique portion angle are 142 ± 0.5 ° (referring to Fig. 4), and the straight portion of the 3rd pair of spinous process steel plate 6 and oblique portion angle are 154 ± 0.5 ° (referring to Fig. 5).
Referring to Fig. 6, shown in 7, according to rat of measuring the 4th and fifth lumbar vertebra spinous process L
4-L
5Sections, the 5th and the 6th spinous process of lumbar vertebra L
5-L
6Distance between the sections, the length of the said first and second connection steel plates 1,3 is that 15mm, width are that 5mm, thickness are 1.2mm.The through hole that connects on the steel plate 1,3 is that diameter is the circle of 2 ± 0.05mm, and the two ends of long through-hole are semicircle, and its diameter is 2 ± 0.05mm, and the centre of long through-hole is a rectangle, the long 2mm of rectangle part, high 2 ± 0.05mm.The through hole of the first connection steel plate 1 and the centre-to-centre spacing of long through-hole are 7.26 ± 0.2mm, and the through hole of the second connection steel plate 3 and the centre-to-centre spacing of long through-hole are 7.61 ± 0.2mm.
Referring to shown in Figure 8, first screw 9 is implanted in the rat body, is M1 socket cap in-line screw, reach 7.0mm, and screw total length 8.5mm, the socket cap diameter of screw is 4mm.
Referring to shown in Figure 9, second screw 8 is a M2 socket cap in-line screw, and reach is 8.0mm, screw total length 9.5mm, and the socket cap diameter of screw is 4mm.
Referring to shown in Figure 10, the 3rd screw 10 is a M2 socket cap in-line screw, reach 14.0mm, and screw total length 15.5mm, the socket cap diameter of screw is 4mm.
The profile of said first and second nuts 2,7 is square or hexagon.
Below in conjunction with an embodiment, specify the method that device of the present invention is made rat vertebral subluxation animal model of using.
1, animal for research
Male SD rat (cleaning level), body weight 350g～450g.
2, operating theater instruments and medicine
2.1, apparatus
Mosquito forceps (little) curved forceps * 2 friction clamp * 2
One on handle of a knife
One on blade
One of needle holder
Tissue is cut two
Two of loopers
Two on screwdriver (self-control)
External fixation system one pair (self-control)
Cotton ball soaked in alcohol is some
Sterilized cotton ball is some
Iodine furan cotton balls is some
2.2, medicine
Ketaject injection, gentamicin injection liquid, compound recipe are to acetamido acid sheet (the sharp pain of loosing), 75% ethanol, iodine furan and 0.9% normal saline.
3, make flow process
3.1, the preparation before making
3.1.1, preserved skin
Preceding 8 hours fasting water of rat art.The bigger regional warm water cleaning of rat lower back, electric shaver is shaved and is removed by hair (avoiding epidermis injury), and operative region cleans with warm suds, and the ether degrease is wiped away and is done the back iodophor disinfection, and 75% ethanol takes off iodine.
3.1.2, operating theater instruments prepares
Above-mentioned operating theater instruments art high pressure steam sterilization the previous day.
3.2, implementing procedure
1. anesthesia is with fixing: rat abdominal cavity ketamine injecting anesthetic (120mg/kg), about two minutes of injection back treats after rat gets into narcotism its ventricumbent position to be fixed on the operating-table.Operative region and peripheral 3cm iodophor disinfection, 75% ethanol takes off iodine (referring to Figure 11);
2. drape: the operative region drape is to keep the operative region cleaning;
3. locate: locate the 6th spinous process of lumbar vertebra L
6Sections (flat to crista iliaca) is upwards located fifth lumbar vertebra spinous process L successively
5Sections, fourth lumbar vertebra spinous process L
4Sections.
4. incision of skin: blade along posterior midline from fourth lumbar vertebra spinous process L
40.5cm to the six spinous process of lumbar vertebra L under the sections
6Sections L
60.5cm cuts skin under the spinous process;
5. separate tissue: the mosquito forceps passivity is separated subcutaneous connective tissue, is close to fourth, fifth and six spinous process of lumbar vertebra L with blade
4-L
6Sections is other to be separated spinous process with perpendicular spinal muscle on every side, avoid injuring on zygapophysial joints and anapophysis and the sour jujube and interspinal ligaments and tissue, exposure fourth, fifth and six spinous process of lumbar vertebra L
4-L
6Sections (referring to Figure 12);
6. the steel plate of part is fixed in the body: select for use the 10ml syringe needle to make perforating needle (diameter of the 10ml syringe needle diameter and first screw 9 is more approaching, is convenient to first screw and penetrates), the right hand is held perforating needle, from the 6th spinous process of lumbar vertebra L
6The about 2mm inserting needle in top, sections basilar part center; Get out a hole (the about 1.5mm of diameter) (referring to Figure 13); With mosquito forceps clamping first screw 9, be inserted in the 3rd a pair of spinous process steel plate 6, first screw 9 is aimed at hole; Hold screwdriver first screw slowly is screwed into (be screwed in the process to keep first screw 9 and screwdriver vertical with spinous process) along hole, pass about 3mm from the spinous process offside to first screw 9.The 3rd pair of spinous process steel plate 6 of another piece is inserted in first screw 9 that passes; Then aim at first screw 9 with mosquito forceps clamping first nut 7; Twist the first tight screw 9 (prevent in this process firmly excessive, otherwise be prone to cause spinous process fracture or necrosis) with screwdriver, the 3rd pair of spinous process steel plate 6 fixedly finishes.The rest may be inferred for the implantation process of second pair and first pair spinous process steel plate 5,4 (referring to Figure 14,15).
7. muscular tissue is sewed up: between adjacent steel plate, adopt the interrupted suture method to sew up perpendicular spinal muscle in both sides and connective tissue on every side.
8. outer body is fixing: mosquito forceps clamping first connects steel plate 1 and places (manhole of the first connection steel plate 1 is corresponding with the counterbore of the first spinous process steel plate, 4 straight portions) between two blocks of first pair of spinous process steel plates 4; Second screw 8 is penetrated; With 2 lockings of second nut, first pair of spinous process steel plate 4 is connected steel plate 1 with first fixing.The second connection steel plate 3 between two blocks of the 3rd pair of spinous process steel plates 6, is locked with second screw 8 and second nut 2 then.The long through-hole end that the first connection steel plate 1 is connected steel plate 3 with second places between two blocks of second pair of spinous process steel plates 5, then with the 3rd screw 10 and second nut 2 fixing (referring to Figure 16-18).
9. clean wound: field of operation uses gentamycins (gentamycin 2ml+0.9% normal saline 8ml) flushing repeatedly for three times, and the interrupted suture skin suture is adopted in thoroughly hemostasis by compression between the adjacent spinous steel plate.
(7) post surgery treatment
1. the irradiation of postoperative electric filament lamp is 1 hour, impels rat from narcotism, to revive early.
2. behind the recovery from anesthesia, rat is put into the single cage of modified model rat feeding cage device raise.
3. three days intramuscular injection gentamycins (gentamycin 2ml+0.9% normal saline 8ml) of postoperative.
4. add compound paracetamol tablets (the sharp pain of loosing) in the water bottle that rat is fed, press 300mg/kg/d and give amount, continuous three days of postoperative;
5. analgesic was fed the rat pain behavior of observing after three days (appetite depression, the time lengthening that reposes, scratch or active situation) two days.
6. experimental rat has the convalescent period about an above-mentioned week.
(8) animal for research is fed
Postoperative, laboratory animal place single cage raising in the rat feeding cage device.For guaranteeing the outside normal condition that connects fixture, conventional rearging cage is carried out limited improvement.The rustless steel of placing food with corrosion resistant plate replacement with holes shrouds, and guarantees the circulation of air.Simultaneously; Design " ∠ " shaped steel plate, on the steel plate inclined-plane, reserved portalling of food and water, the height that portals through continuous adjustment and the angle of inclination on steel plate inclined-plane; When making acquisition food that rat can be freely and moisture, play the effect of partial simulation biped behavior.
Embodiment
Purpose: observe the outside fixture that connects and implant the influence of back to the rat spine function.
1, animal for research
36 of male SD rats (cleaning level), body weight 350g～450g.
2, laboratory animal is divided into groups
The male SD rat (cleaning level) of 36 heavy 350g～450g is divided into experimental group (F group at random; The outside fixture that connects of implantation), matched group (C group; Implant the spinous process steel plate, but the straight portion of spinous process steel plate does not connect steel plate), sham operated rats (the K group is not implanted outside connection fixture)
Experimental group is divided into by design length observing time at random observes a week group (F1), has three observation units to be respectively F11, F12, F13 in the group; Observe two week groups (F2), have three observation units to be respectively F21, F22, F23 in the group; Group (F4) has three observation units to be respectively F41, F42, F43 around observing in the group; With observation eight week groups (F8), there are three observation units to be respectively F81, F82, F83 in the group.
Matched group is divided into by design length observing time at random observes a week group (C1), has three observation units to be respectively C11, C12, C13 in the group; Observe two week groups (C2), have three observation units to be respectively C21, C22, C23 in the group; Group (C4) has three observation units to be respectively C41, C42, C43 around observing in the group; With observation eight week groups (C8), there are three observation units to be respectively C81, C82, C83 in the group.
Sham operated rats is divided into by design length observing time at random observes a week group (K1), has three observation units to be respectively K11, K12, K13 in the group; Observe two week groups (K2), have three observation units to be respectively K21, K22, K23 in the group; Group (K4) has three observation units to be respectively K41, K42, K43 around observing in the group; With observation eight week groups (K8), there are three observation units to be respectively K81, K82, K83 in the group.
3, experimental animal model is made
1. the experimental group animal carries out modelling according to foregoing method.
2. matched group surgical procedure: matched group does not have the connection that outer body connects steel plate and fixes all the other steps identical with experimental group (referring to Figure 19).
3. sham operated rats surgical procedure: the implantation of no spinous process steel plate is connected the connection of steel plate to be fixed with outer body, other steps identical with experimental group (referring to Figure 20).
Postoperative processing of all laboratory animals and nursing are all handled according to foregoing method.
4, observation index
The X line is measured vertebral body trailing edge angle (PBA) after taking the photograph sheet
(1) the X line is taken the photograph the sheet timing node
Experimental group, matched group and each observation unit of sham operated rats animal are taking lumbar X line lateral projection respectively for twice first with before the test of last vertebral rigidity.
(2) the X line is taken the photograph sheet equipment
Faxitron?Specimen?Radiography?System
(3) X line photography method
1. took the photograph preceding all the rat fasting water of sheet one day (reducing intestinal contents) to taking the photograph the influence of tablet quality.
2. rat ketamine intraperitoneal injection of anesthesia (120mg/kg).
3. opening Faxitron Specimen Radiography System will take the photograph the sheet standard and set up and to be 10s, 31kv, projection distance 57.2cm.
4. will anaesthetize good rat and put into X-ray machine, fifth lumbar vertebra spinous process L
5Sections is aimed at the center of projection point, and when taking lateral projection, rat nature lateral position when taking the hyperextension position, is put into self-control rat hyperextension position stationary fixture (EPJ) with rat, with the angle that the fixing rat back of the body is stretched, takes the photograph sheet after position adjustment finishes.
5. take out rat after taking the photograph sheet, data deposit computer automatically in.
(4) vertebral body trailing edge angle (PBA) measuring method
The the 4th and the 6th spinous process of lumbar vertebra L
4, L
6Sections vertebral body trailing edge angle: in PACS (radiation image processing system) system, measuring vertebral body trailing edge angle (PBA) measuring method is: respectively from the 4th and the 6th spinous process of lumbar vertebra L
4And L
6The vertebral body trailing edge draws straight line, and these two angles that straight line became (acute angle) are the vertebral body trailing edge angle.
5 results
Rat vertebral body trailing edge angle (PBA) by two along fourth lumbar vertebra spinous process L
4Vertebral body trailing edge and the 6th spinous process of lumbar vertebra L
6Vertebral body straight line that trailing edge is done intersects the angle (acute angle) that is become and constitutes.Be set in rat the 4th to the 6th spinous process of lumbar vertebra L
4-L
6When becoming flexing, stipulates the vertebral body sections that this angle value is on the occasion of (+), if opposite rat the 4th to the 6th spinous process of lumbar vertebra L
4-L
6This angle block gauge was decided to be negative value (-) when the vertebral body sections became the back of the body to stretch state.Experimental group, matched group, sham operated rats PBA angle-data under hyperextension state after the observation is added up as follows:
Experimental group, matched group and sham operated rats PBA angle have significant difference through rank test P＜0.01 of a plurality of independent samples, and the rank test of comparing in twos between each group P value as a result all has significant difference less than 0.01.
From Figure 21 can find out experimental group externally during fixing dry prognosis hyperextension position the PBA angle on the occasion of, the 4th to the 6th spinous process of lumbar vertebra L is described
4-L
6Spinal segments still is in flexion, and the external stability connection defines the 4th to the 6th spinous process of lumbar vertebra L
4-L
6The joint motion of spinal segments, joint are in the dysfunction state that experiment is envisioned.And matched group is not because external fixation system has connection, the 4th to the 6th spinous process of lumbar vertebra L
4-L
6The spinal segments joint motion is unrestricted, so this variation is not obvious when the hyperextension position.Sham operated rats the 4th to the 6th spinous process of lumbar vertebra L
4-L
6Spinal segments is in normal hyperextension state when the hyperextension position.
More than, the present invention has been carried out detailed explanation, but protection scope of the present invention is not limited to described embodiment through embodiment.Under the situation that does not break away from the principle of the invention, those skilled in the art also can make many distortion and improvement, and these also should be regarded as protection scope of the present invention.
Claims (5)
1. an outside that is used to make rat vertebral subluxation animal model connects fixture; The material of all parts and screw-nut all adopts orthopaedics to use rustless steel, it is characterized in that: said device is made up of three pairs of spinous process steel plates, two blocks of connection steel plates and a plurality of screw and nut;
Every pair of spinous process steel plate all has curved oblique portion of portion and this straight portion of edge always to the right; Angle between the straight portion of first pair of spinous process steel plate and the oblique portion is less than the straight portion of second pair of spinous process steel plate and the angle between the oblique portion, and the angle between the straight portion of second pair of spinous process steel plate and the oblique portion is less than the straight portion of the 3rd pair of spinous process steel plate and the angle between the oblique portion; Above-mentioned each to the identical length of the straight portion of spinous process steel plate and oblique portion etc.; Straight portion upper end at every pair of spinous process steel plate is provided with a through hole, and the roughly centre position that is positioned at said straight portion is provided with second through hole, and the oblique portion that is positioned at every pair of spinous process steel plate is provided with third through-hole;
The oblique portion of first pair of spinous process steel plate grips rat fourth lumbar vertebra spinous process sections, and the oblique portion of second pair of spinous process steel plate grips rat fifth lumbar vertebra spinous process sections, and the oblique portion of the 3rd pair of spinous process steel plate grips rat the 6th spinous process of lumbar vertebra sections; First screw and first nut are fixed the oblique portion of every pair of spinous process steel plate with corresponding spinous process sections; The oblique portion of each spinous process steel plate matches with the angle that becomes of spinous process with vertebral body; The straight portion and the horizontal line of each spinous process steel plate are perpendicular;
The straight portion of first pair of spinous process steel plate is connected steel plate with first, and the straight portion of the 3rd pair of spinous process steel plate is connected steel plate with second, all adopts second screw to fixedly connected with second nut; The straight portion of second pair of spinous process steel plate is connected the long through-hole end that steel plate is connected with second on the steel plate fixedlys connected with second nut with the 3rd screw with first, and second connects steel plate and be positioned at first upper end that connects steel plate.
2. the outside fixture that connects as claimed in claim 1, it is characterized in that: described spinous process steel plate specification is following: straight minister's degree is 15mm, and tiltedly minister's degree is 5.5mm, launches long 20.5mm, and width is 4.0mm, and thickness is 1.2mm; Wherein, the angle between the straight portion of said first pair of spinous process steel plate and the oblique portion is 138 ± 0.5 °; Angle between the straight portion of said second pair of spinous process steel plate and the oblique portion is 142 ± 0.5 °, and the angle between the straight portion of said the 3rd pair of spinous process steel plate and the oblique portion is 154 ± 0.5 °.
3. according to claim 1 or claim 2 outside connects fixture, it is characterized in that: said first to connect steel plate is connected steel plate with second length be that 15mm, width are that 5mm, thickness are 1.2mm; The said first connection steel plate is connected steel plate with second a end is provided with the through hole that a diameter is 2 ± 0.05mm; The other end that the said first connection steel plate and second is connected steel plate is provided with a long through-hole, and the two ends of this long through-hole are semicircle, and its diameter is 2 ± 0.05mm, and the centre of long through-hole is a rectangle, the long 2mm of this rectangle part, high 2 ± 0.05mm; The through hole of the first connection steel plate and the centre-to-centre spacing of long through-hole are 7.26 ± 0.2mm, and the through hole of the second connection steel plate and the centre-to-centre spacing of long through-hole are 7.61 ± 0.2mm.
4. according to claim 1 or claim 2 outside connects fixture, it is characterized in that:
Said first screw is a M1 socket cap in-line screw, and reach is 7.0mm, screw total length 8.5mm, and the socket cap diameter of screw is 4mm;
Said second screw is a M2 socket cap in-line screw, and reach is 8.0mm, screw total length 9.5mm, and the socket cap diameter of screw is 4mm;
Said the 3rd screw is a M2 socket cap in-line screw, and reach is 14.0mm, screw total length 15.5mm, and the socket cap diameter of screw is 4mm;
The profile of said first nut and second nut is square or hexagon.
5. the outside fixture that connects as claimed in claim 3 is characterized in that:
Said first screw is a M1 socket cap in-line screw, and reach is 7.0mm, screw total length 8.5mm, and the socket cap diameter of screw is 4mm;
Said second screw is a M2 socket cap in-line screw, and reach is 8.0mm, screw total length 9.5mm, and the socket cap diameter of screw is 4mm;
Said the 3rd screw is a M2 socket cap in-line screw, and reach is 14.0mm, screw total length 15.5mm, and the socket cap diameter of screw is 4mm;
The profile of said first nut and second nut is square or hexagon.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008100435953A CN101617959B (en) | 2008-07-04 | 2008-07-04 | External connection fixing device for manufacturing rat spine subluxation animal model |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008100435953A CN101617959B (en) | 2008-07-04 | 2008-07-04 | External connection fixing device for manufacturing rat spine subluxation animal model |
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| CN101617959A CN101617959A (en) | 2010-01-06 |
| CN101617959B true CN101617959B (en) | 2012-05-02 |
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| CN2008100435953A Active CN101617959B (en) | 2008-07-04 | 2008-07-04 | External connection fixing device for manufacturing rat spine subluxation animal model |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102342856A (en) * | 2010-07-29 | 2012-02-08 | 上海中医药大学附属曙光医院 | Centrum external fixing device for making animal model, making method and auxiliary tool |
| CN112190321A (en) * | 2020-05-26 | 2021-01-08 | 陆金明 | Minimally invasive chain type stabilizing device for lumbar artificial slippage |
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| CN101617959A (en) | 2010-01-06 |
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