CN101354398B - Carrier for containing detection reagent strip - Google Patents
Carrier for containing detection reagent strip Download PDFInfo
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- CN101354398B CN101354398B CN 200810120955 CN200810120955A CN101354398B CN 101354398 B CN101354398 B CN 101354398B CN 200810120955 CN200810120955 CN 200810120955 CN 200810120955 A CN200810120955 A CN 200810120955A CN 101354398 B CN101354398 B CN 101354398B
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Abstract
The invention provides a carrier used for receiving detecting reagent bar, comprising a clamping trough used for supporting the detecting reagent bar; the clamping trough comprises a structure which blocks the flowing of liquids outside the detecting reagent bar on the clamping trough. The carrier of the invention has the beneficial effects that a space is arranged between the clamping trough and the detecting reagent bar so as to block the flowing of liquids outside the detecting reagent bar on the clamping trough, thus leading the flowing of the liquid sample on the detecting reagent bar to be consistent, preventing the generation of the line-breaking phenomenon and improving the sensitiveness and the exactness of the detection.
Description
Technical field
The present invention relates to a kind of carrier of containing detection reagent strip, especially the storing apparatus of the detection reagent strip in the quick diagnosis field.
Background technology
At present, for detection of the pick-up unit that whether contains analyte in the sample, be used in a large number hospital or family, these pick-up units that are applied to quick diagnosis comprise one or more and detect reagent strip, detect such as early pregnancy, and drug abuse detects etc.The pick-up unit of this quick diagnosis is very convenient, can at one minute, perhaps obtain testing result at the detection reagent strip about ten minutes at the most.
In these pick-up units, detect reagent strip and generally be positioned on certain carrier.Carrier can be the mould of plate shape or plate etc.Carrier generally is made of draw-in groove, and a draw-in groove can hold one and detect reagent strip.On the pick-up unit of using at present, on the surveyed area that detects reagent strip, we usually can read the zone in testing result and see that lines are inhomogeneous, and are deep mixed, or even the only phenomenon that exists of some lines, namely usually said " broken string " phenomenon.As shown in Figure 1, be the lines of normal detection line among the N figure, and it is wired to have occurred two ends among the A figure on the detection line, the centre does not have the band of line; Wired in the middle of then having occurred among the B figure, two ends do not have the situation of line; The band depth is inhomogeneous among the C figure.Above-mentioned " broken string " situation can cause all that to the misreading of testing result it is positive or negative making people's indistinguishable result.
Summary of the invention
In order to solve problems of the prior art, the invention provides a kind of like this carrier for containing detection reagent strip, comprise the draw-in groove of supporting detection reagent strip, comprise on the draw-in groove that blocking-up detects the structure that reagent strip liquid in addition flows at draw-in groove.
Preferably, described blocking-up structure comprises that width is greater than the draw-in groove that detects reagent strip.Preferred, the Width of draw-in groove detects the wide at least 1mm of reagent strip.Preferred in addition, draw-in groove is than detecting between the wide 1mm to 15mm of reagent strip.Draw-in groove can also comprise fence.
Preferred in addition, there is draw-in groove in blocking-up structure and detects the adsorptive pads of reagent strip, between label pad or the surveyed area.
Preferred in addition, blocking-up structure comprises the opening that is positioned on the draw-in groove.More preferred, the corresponding at least marked region that detects reagent strip of opening.
In other concrete scheme, draw-in groove also comprises makes the fixed fixed part of described detection reagent strip.Preferably, fixed part is used for the suction zone of fixed test reagent strip.More preferred, fixed part comprises the projection that detects reagent strip for clamping.
In addition, can also comprise on the carrier that makes the upright supporter of carrier.
The invention has the beneficial effects as follows: by between draw-in groove and detection reagent strip, the space being set, stop detection reagent strip liquid in addition to flow at draw-in groove, make the mobile uniformity of the liquid sample that detects on the reagent strip, prevent the generation of " broken string " phenomenon, improve the sensitivity and the accuracy that detect.
Description of drawings
Fig. 1 is the synoptic diagram that has the detection reagent strip of " broken string " phenomenon and show positive positive normal result's detection reagent strip;
Fig. 2 is the planimetric map of one embodiment of the invention;
Fig. 3 is that embodiment among Fig. 2 is with the planimetric map that detects reagent strip;
Fig. 4 is the stereographic map with the urine cup of the described embodiment of Fig. 2;
Fig. 5 is explosive decomposition figure embodiment illustrated in fig. 4;
Fig. 6 is the synoptic diagram of an alternative embodiment of the invention;
Fig. 7 is schematic rear view embodiment illustrated in fig. 6;
Fig. 8 be among Fig. 6 embodiment with the synoptic diagram that detects reagent strip;
Fig. 9 is the stereographic map with the urine cup of embodiment shown in Figure 6;
Figure 10 is explosive decomposition figure embodiment illustrated in fig. 9.
Description of reference numerals: detect reagent strip 100, sample receiving pad 101, label pad 102, surveyed area 103, adsorptive pads 104, control line 105, detection line 106; The first urine cup 2, test card 20, carrier 200, draw-in groove 201, fence 202, opening 203, projection 204, bakie 205, bowl cover 21, lid holder 210, caping 211, push rod 212, cup 22, collection well 221, the cup end 222, piston 223, plunger shaft 224, test chamber 225, pick-up unit 23, test chamber lid 231, panel 232; The second urine cup 3, test card 30, plate carrier 30 as one kind 0, draw-in groove 301, fence 302, opening 303, projection 304, rubber strip 306, heel 307, bowl cover 31, bowl cover screw thread 310, cup 32, collection well 321, test chamber 322, cup screw thread 323.
Embodiment
The below is described further structure or these the employed technical terms that the present invention relates to.
Detect
Detect the expression chemical examination or test a kind of material or whether material exists, such as, but be not limited to this, the metabolin of chemical substance, organic compound, mineral compound, metabolism product, medicine or drug metabolite, organic organization or organic organization, nucleic acid, protein or polymkeric substance.In addition, detect the quantity of expression test substances or material.Furtherly, chemical examination also represents immune detection, chemical detection, enzyme detection etc.
Sample
The sample of indication of the present invention refers to that those can be used for detecting, chemically examining or diagnose the material that whether has interested analyte.Sample can be that for example, liquid sample, liquid sample can comprise blood, blood plasma, serum, urine, saliva and various juice, can also comprise solid sample and the semi-solid samples liquid solution through forming after anticipating.The sample of collecting can be used for the methods such as immune detection, chemical detection, enzyme detection and detect whether there is analyte.
Detect reagent strip
Applying to detection reagent strip 100 of the present invention can be usually said horizontal effluent reagent strip 100, and these detect the concrete structure of reagent strip 100 and detect principle is the known technology of persons skilled in the art in the prior art.Common detection reagent strip 100, comprise the sample collection zone, marked region 102, surveyed area 103 and suction zone 10.4, the sample collection zone comprises sample receiving pad 101, marked region 102 comprises label pad 102, and suction zone 104 can comprise adsorptive pads 104, wherein comprises on the surveyed area 103 and can detect the necessary chemical substance that whether contains analyte.General commonly used detection reagent strip 100 is nitrocellulose filter reagent strip 100, and namely surveyed area 103 comprises nitrocellulose filter, nitrocellulose filter fixedly the specific bond molecule show the result of detection; Can also be cellulose acetate membrane or nylon membrane etc.
Analyte
The example of the analyte that relates among the enough the present invention of energy comprises some haptens materials, and these haptens comprise drugs (such as drug abuse)." drug abuse " (DOA) refers to that non medical goal ground uses medicine (it is neural usually to play paralysis).Abuse these medicines and can cause body ﹠ mind to suffer damage, produce dependence, habit-forming and/or dead.The example of drug abuse comprises cocaine; Amphetamine AMP (for example, black beauty, white amphetamine tablet, dextroamphetamine, dexie, Beans); Crystal methamphetamine MET (crank, meth, crystal, speed); Barbiturate BAR (such as Valium, Roche Pharmaceuticals, Nutley, New Jersey); Sedative (paramedicines of namely sleeping); Lysergic acid diethylamide (LSD); Inhibitor (downers, goofballs, barbs, blue devils, yellow jackets, methaqualone); The anti-antidepressant of tricyclic antidepressants (TCA, i.e. imipramine, amitriptyline and doxepin); Diformazan dioxy ylmethyl aniline MDMA; Hog (PCP); Tetrahydrocannabinol (THC, pot, dope, hash, weed, etc.); Opiate (be morphine MOP or, opium, cocaine COC; , heroin, the hydroxyl dihydrocodeinone); Anxiolytic and hypnotic sedative agent, anxiolytic is that a class is mainly used in anxiety reduction, nervous, frightened, set the mind at rest, have the medicine of hypnosis sedation concurrently, comprise Benzodiazepines BZO (benzodiazepines), atypia BZ class, merge phenodiazine NB23C class, the tall and erect class of benzene nitrogen, the part class of BZ acceptor, open loop BZ class, diphenylmethane derivatives, the piperazine carboxylic acid salt, the piperidine carboxylic acid salt, Kui azoles quinoline ketone, thiazine and thiazole, other heterocyclic, imidazole type calmness/anodyne (such as hydroxyl dihydrocodeinone OXY, methadone MTD), propanediol derivative-carbamates, fatty compound, anthracene derivative etc.Use the pick-up unit of the present invention also can be for detection of belonging to medical usage but the easily detection of overdose, such as tricyclic antidepressant (imipramine or analog) and Paracetamol etc.Can resolve into different small-molecule substances after these medicines are absorbed by the body, these small-molecule substances are present in the body fluid such as blood, urine, saliva, sweat or there is above-mentioned small-molecule substance in part body fluid.
Pick-up unit
Pick-up unit refers to for detection of the device that whether contains analyte in the sample.In the present invention, pick-up unit especially represents for the pick-up unit in the quick diagnosis field, as detecting reagent strip 100, test card 20, test bar, the various forms of pick-up units such as detection cup.Pick-up unit can comprise test card 20 and and test card 20 matching used parts, as shown in Figure 4, urine cup 2 can be used as a kind of form of pick-up unit, in general, urine cup 2 can comprise collecting zone 221 and test card 20, and test card 20 can and detect reagent strip 100 by carrier 200 and jointly form.Applying to detection reagent strip 100 of the present invention can be usually said horizontal effluent reagent strip 100, the pick-up unit that applies among the present invention is to have comprised the test card 20 of reagent strip 100 or detected cup 2 (or claiming urine cup), and the concrete structure of these reagent strips 100 or test card 20, urine cup 2 etc. and detection principle are the known technology of persons skilled in the art in the prior art.
In the present detection, this cassette or cup type pick-up unit all needs carrier 200 of detection reagent strip 100 usefulness is supported, the function that competence exertion detects.Yet, in existing field, often produce " broken string " phenomenon, affect reading of testing result.Especially near the cut-off value, the signal on detection line 106 is original just more weak, if there is " broken string " phenomenon to exist, just difficulty judges that testing result is " positive " or " feminine gender " on earth again.We find, detect between reagent strip 100 and the carrier 200 and usually closely connect, cause extremely easily capillarity, producing detection reagent strip 100 liquid in addition flows at draw-in groove 201, as detecting between reagent strip 100 and draw-in groove 201 both sides, perhaps detect between reagent strip 100 behinds and the draw-in groove 201.This liquid flow is different from the liquid flow that detects on the reagent strip 100, is easy to infiltrate one step ahead and detects reagent strip 100, will cause like this breaking or causing that the lines of generation are inhomogeneous.Especially, in case the mark substance zone of detecting on the detected reagent strip 100 of reagent strip 100 liquid sample in addition absorbs, the mark substance that detects on the reagent strip 100 will be dissolved first, causes the dissolving of mark substance inhomogeneous.The mark substance that liquid beyond the detected reagent strip 100 dissolves can flow along detecting reagent strip 100, arrives surveyed area 103 also and the raw material combination on the detection line 106.At this moment, part liquid (detect reagent strip on) sample also not with the raw material combination, another part liquid (detecting beyond the reagent strip) is gone ahead of the rest, and combine raw material, it is deep mixed to form lines at the detection line 106 of surveyed area 103, perhaps lines are inhomogeneous, or even the only phenomenon that exists of some lines, namely usually said " broken string " phenomenon.
In order to overcome this defective, the present invention adopts such carrier 200, can block and detect reagent strip 100 liquid in addition flowing on draw-in groove 201, furtherly, can block liquid from the both sides of detection reagent strip 100 or flowing of the back side, thereby prevent the generation of " broken string " phenomenon.
Carrier
Comprise on the draw-in groove 201 that blocking-up detects the structure that reagent strip 100 liquid in addition flows at draw-in groove 201.In theory, liquid sample only can transmit and flow detecting reagent strip 100 when detecting.And other spaces of draw-in groove 201, such as the space, both sides between draw-in groove 201 and detection reagent strip 100, and on the space between detection reagent strip 100 behinds and the draw-in groove 201, there is not or seldom exists the phenomenon of liquid flow, when even liquid flow is arranged, also can be blocked the structure blocking-up, be unlikely to make the combinations such as liquid and mark substance.
This blocking-up structure can be that draw-in groove 201 is own, such as the draw-in groove 201 of width greater than detection reagent strip 100.Preferably, the width of draw-in groove 201 is greater than the width that detects reagent strip 100, and the cooperation of this form can produce the space between draw-in groove 201 and reagent strip 100, and the existence in this space can reduce the capillarity that detects reagent strip 100 both sides.Like this, accept in the process of sample at detection reagent strip 100, liquid sample can only along the sample region of acceptance 101 of detecting reagent strip 100, transmit liquid to other positions of detecting reagent strip 100.In the prior art, because the distance that detects between reagent strip 100 and the draw-in groove 201 is very little, tiny space between them can produce very strong capillary action, so that a part of liquid sample does not circulate by detecting reagent strip 100, but flow by the space of detecting between reagent strip 100 and the draw-in groove 201.Like this, can cause the liquid sample that detects on the reagent strip 100 to transmit unevenly, cause that mark substance contacts with sample near insufficient consequence, especially the cut-off value such as inhomogeneous, be more prone to cause " broken string " phenomenon, cause misreading of result.Such as Fig. 2 and shown in Figure 6, the width that draw-in groove 201 is set is greater than the width that detects reagent strip 100, and width between the two be so that capillary action can't produce, and only uniformity ground flows on the reagent strip 100 detecting can to make liquid sample.The width of draw-in groove 201, than detecting reagent strip 100 wide 0.5mm, 0.6mm, 0.8mm, can also be than detecting reagent strip 100 wide 1mm or more.Preferably, the Width of draw-in groove 201 detection reagent strip 100 wide scopes are 1mm-15mm.In detail, draw-in groove 201 can be than detecting reagent strip 100 wide 1mm, 1.2mm, 1.4mm, 1.6mm, 1.8mm, 2mm, 2.4mm, 2.8mm or 3mm, 4mm, 6mm, 8mm, 10mm, 12mm, 14mm, 15mm or larger.More preferred, the various piece of draw-in groove 201 and the width distance that detects between the reagent strip 100 are inhomogeneous.For example, the clamping of the adsorptive pads 104 of the top of draw-in groove and detection reagent strip 100 is more closely, but detect sample receiving pad 101, label pad 102 and the surveyed area 103 of reagent strip 100, and have the distance that can prevent capillarity between the draw-in groove 201.The carrier 200 of this form, clamping detects reagent strip 100 effectively on the one hand, and it is maintained static, and also can also prevent capillarity at critical area on the other hand.
This blocking-up structure not only can be draw-in groove itself, also can be a certain parts that are positioned on the draw-in groove.Detect the liquid communication that the capillarity between reagent strip 100 and the draw-in groove 201 produces, also there are other positions, as detect between reagent strip 100 behinds and the draw-in groove 201, if detecting reagent strip 100 is close on the draw-in groove 201, when having liquid sample to exist, liquid sample is easy to by the behind of detecting reagent strip 100 and the finedraw between the draw-in groove 201, produce very strong capillary action, this part liquid can be with than faster speed transmission, mark substance on also can incorporation of markings zone 102,106 form obvious or fuzzy lines on detection line, affect the judgement of testing result, sensitivity and accuracy that final impact detects.Blocking-up detects the capillarity between reagent strip 100 and the draw-in groove 201, can also by the mode in draw-in groove 201 upper sheds 203, comprise on the draw-in groove 201 that namely the liquid beyond the blocking-up detection reagent strip 100 is opening 203 in the structure that draw-in groove 201 flows.Opening 203 can provide a breach at liquid sample when mobile, so that not smooth being stagnated of flowing of liquid.Opening 203 can be positioned at any position of draw-in groove 201.The quantity of opening 203 can be 1,2, and 3 or a plurality of.Such as Fig. 2, Fig. 6 and shown in Figure 7, preferred, comprise an opening 203 on each draw-in groove 201.Preferably, the position of opening 203 is positioned at the bottom of draw-in groove 201, like this, in the time of from liquid sample contact draw-in groove 201, just has been blocked.Preferred in addition, opening 203 is positioned at the Lower Half of draw-in groove 201, can just block the liquid flow that capillarity produces like this when liquid does not flow to surveyed area 103.More preferred, the position of opening 203 on draw-in groove 201 is corresponding to detecting near the position the marked region 102 on the reagent strip 100.The opening of this form can effectively prevent liquid sample because capillarity and marked region 102 produce circulation.More preferred, the length of the big or small greater than flag pad 102 of opening is so that the mark substance on the maximum magnitude ground protection label pad 102 avoids mark substance to contact with improper flowing liquid sample.Better, the position of opening 203 is positioned on draw-in groove 201 positions corresponding with the sample region of acceptance 101 of detecting reagent strip 100.The opening 203 of this form, in sample region of acceptance 101, detect reagent strip 100 liquid communication behind in sample flow, just be blocked, thereby can not produce capillarity at this position, can guarantee that liquid sample is in the proper flow that detects on the reagent strip 100.More preferred, this opening 203 is for being positioned at the rectangular aperture 203 of draw-in groove 201 bottoms.This rectangular aperture 203 can be wide, more preferred with detection reagent strip 100, and this opening 203 and draw-in groove 201 are wide.This kind opening 203 is set, can makes the liquid flow that detects on the reagent strip 100 compare uniformity.
This blocking-up structure can also be realized by other means.For example, only in a part of place of draw-in groove 201 fence 202 is set, preferably, on the top of draw-in groove 201 fence 202 is set, is used for clamping and detects reagent strip 100, make it be in stationary state, bottom at draw-in groove 201, or/and surveyed area 103 does not arrange fence 202, make the detection that detects reagent strip 100 be in not disturbed state corresponding to the marked region 102 that detects reagent strip 100.Prevent from detecting reagent strip 100 and the capillary blocking-up structure between the draw-in groove 201 behind, can also the parts that whippletree etc. can supporting detection reagent strip 100 be set at draw-in groove 201, make and detect reagent strip 100 " frame " on draw-in groove 201.Make in this way reagent strip 100 be in " soaring aloft " state, so just can not produce detection reagent strip 100 liquid in addition and flow at draw-in groove 201.Further, detect reagent strip 100 and be in the state that " frame " rises, detect so reagent strip 100 and itself not too can be subjected to the impact of extraneous carrier 200 or housing, detection sensitivity and accuracy also can keep higher level, especially near the cut-off value, can show more normal testing result symbol, be not easy to cause result's the erroneous judgement of misreading.
In addition, such as Fig. 2, Fig. 6 and shown in Figure 8 can also comprise on the draw-in groove 201 making and detect reagent strip 100 fixed fixed parts.Preferably, this fixed part is used for the suction zone 104 of fixed test reagent strip 100.Wide such as draw-in groove 201 and detection reagent strip 100 in suction zone 104 parts that detect reagent strip 100, clamping detects reagent strip 100 so tightly, but to detecting other parts on the reagent strip 100, such as surveyed area 103, marked region 102 and sample region of acceptance 101 do not produce any impact, the draw-in groove 201 that this part " is widened ", can block surveyed area 103, liquid beyond marked region 102 or the sample region of acceptance 101 flows at draw-in groove 201, be difficult for producing " broken string " phenomenon, like this, just can not misread testing result.Preferred in addition, fixed part comprises that clamping detects the projection 204 of reagent strip 100, and this projection 204 can clamping detects the part of reagent strip 100, as detecting the surveyed area 103 on the reagent strip 100, marked region 102, sample region of acceptance 101 or suction zone 104 etc.More preferred, such projection 204 can clamping detects certain point on the reagent strip 100, such as certain the some position on surveyed area 103 or marked region 102 or the sample region of acceptance 101, by clamping among a small circle, fix and detect reagent strip 100, guarantee again can not cause the liquid flow that detects between reagent strip 100 and draw-in groove 201 both sides.Such projection 204 can be 1 or a plurality of, and is preferred, comprises a pair of corresponding projection 204 on each draw-in groove 201, and more preferred, respectively there is a pair of corresponding projection 204 upper and lower of each draw-in groove 201.
The carrier 200 that is used for containing detection reagent strip 100 among the present invention can independently detect, and also can cooperate other pick-up units to detect.The independent device that uses such as test card 20, is equipped with various detection reagent strips 100 at carrier 200, can be directly used in and measure in the sample whether contain analyte.Preferably, test card 20 cooperates a cover, and the bottom of this carrier 200 divides cooperation, oozes and contaminated environment after complete detecting in order to prevent liquid sample.Preferred in addition, can also comprise on this carrier 200 that makes the upright supporter of carrier 200, can make testing process need not rely on the tester and help by hand or other instruments.The form that cooperates other pick-up units to detect, such as Fig. 4, Fig. 5, Fig. 9 and shown in Figure 10 can comprise test card 20 is assembled to use in the urine cup 2.Test card 20 with carrier 200 and detection reagent strip 100 can be placed among the test chamber 225.Preferably, urine cup 2 also comprises collection well 221, is used for the collection of liquid sample, and this test chamber 225 can be communicated with collection well 221 directly or indirectly, and both are in liquid flowing state.The form of this urine cup 2 has a lot of descriptions in the prior art, is not described in detail at this.
Below in conjunction with concrete accompanying drawing embodiments of the invention is described in detail.These specific embodiment only are limited the enumerating under spirit of the present invention, do not get rid of one of ordinary skill in the art prior art and the present invention in conjunction with and other specific embodiments of producing.
Select a series of DOA products to carry out different detections among the present invention, mainly contain AMP (amphetamine), BAR (barbital), BZO (Benzodiazepine), COC (cocaine), MET (methamphetamine), MDMA (diformazan dioxy ylmethyl aniline), MOP (morphine), OPI (morphine), OXY (hydroxyl dihydrocodeinone), MTD (methadone), PCP (Hog), PPX (the third oxygen is fragrant), TCA (tricyclic antidepressant), THC (tetrahydrocannabinol), COT (nicotine), BUP (buprenorphine), ACE (paracetamol), KET (ketamine), MQL (methaqualone), EDDP (2-acetal-1,5 dimethyl-3,3-Diphenyl Pyrrole alkane).
Embodiment 1
Present embodiment relates to a kind of test card 20.As shown in Figures 2 and 3, this test card 20 comprises carrier 200 and detects reagent strip 100 compositions.Comprise 6 draw-in grooves 201 on this carrier 200, the width of draw-in groove 201 is 5.4mm ± 0.2mm (width that wherein detects reagent strip 100 is 4mm) that greater than detecting reagent strip 100 draw-in groove is than detecting the wide 1.4mm ± 0.2mm of reagent strip.Distance is evenly separated by 5 fence 202 between the draw-in groove 201 between draw-in groove 201 and the draw-in groove 201.A rectangular aperture 203 is set on each draw-in groove 201, the position of opening 203 with detect reagent strip 100 on the position of marked region 102 corresponding.Two pairs of jagged projections 204 are set on each draw-in groove 201, can be used for clamping reagent strip 100, play the effect of stable detection reagent strip 100.Wherein a pair of jagged projection 204 is positioned at the top of draw-in groove 201, and another is positioned at the bottom of draw-in groove 201 to jagged projection 204.In the bottom of each draw-in groove 201, also with the bakie 205 of an arch, unnecessary liquid sample can be spilt from the bakie 205 of arch.Detection reagent strip 100 in this test card 20, for detection of whether containing drug materials in the sample, such as hemp, cocaine etc. material.When the detection, by detecting the front end of reagent strip 100, namely the sample region of acceptance 101, obtain liquid sample, such as urine etc.In liquid sample, kept 1 minute, determine enough samples for detection of.At this moment, liquid sample can be along the sample receiving pad 101 that detects reagent strip 100, arrive label pad 102, fully mix with the mark substance of label pad 102, then arrive surveyed area 103, testing result zone at surveyed area 103 forms detected symbol, such as control line 105 and detection line 106, is used for judging whether liquid sample contains analyte.On the one hand, because the width of draw-in groove 201 leaves certain space greater than the width that detects reagent strip 100 in draw-in groove 201 both sides, make liquid in this space, not form capillarity; On the other hand, in the behind of detecting reagent strip 100, marked region 102 over against draw-in groove 201 positions on have rectangular aperture 203, can effectively must prevent or block liquid sample and produce capillary action in the behind of detecting reagent strip 100 and draw-in groove 201, thereby can not affect the abundant mixing of the mark substance on liquid sample and the marked region 102.
According to test card 20 as above, we have carried out near the testing result experiment the cut-off value, comprise the integrality of sensitivity and T line 106.
1. sensitivity experiment
As shown in Table 1, select respectively 120 test card 20 as statistical experiment.The result shows, the standard that can reach G7 of all ' negative ' specimens (the standard here is from G1 to G8, and the degree that naked eyes can be distinguished is more remarkable, take G4 as boundary), illustrate that naked eyes can unquestionablely distinguish that the testing result of this batch test card 20 is wired, the expression negative findings; Most of concentration can reach the standard of G4 for the-sample of 50%cut-off value after detection, naked eyes can be distinguished has lines to exist on the detection line 106, represent that testing result is negative; Most of concentration also can be less than the standard of G3.5 for the+sample of 50%cut-off value after detection, naked eyes are distinguished does not have lines to exist on the detection line 106, represent that testing result is positive.According to showing in the table two that the result of above-mentioned sensitivity experiment is reasonable, all passed through to detect receptible scope.
Table one, sensitivity experiment
2. integrality experiment
In this experiment, comprise three groups of data, one group is broken string quantity and the outage (table two) of the testing result of negative sample, one group is that concentration is-the pattern detection result's of 50%cut-off value broken string quantity and outage (table three), also has one group to be that concentration is+the pattern detection result's of 50%cut-off value broken string quantity and outage (table four).
Table two shows, in the detection of negative sample, the control experiment of two groups of test card is set, and these two groups of test card are respectively the existing test card (U) of commonly using on the market and the improved test card 20 (I) according to the present invention.Its standard has three, respectively according to the performance level on the detection line 106, a standard is to exist greater than 1/2 on the detection line 106, bar less than 1, another standard is to exist greater than 1/4 on the detection line 106, bar less than 1/2, also having a standard is the bar that exists on the detection line 106 less than 1/4.Experimental result shows do not have improved test card also to have various " broken string " phenomenon, and the test card 20 in the employing present embodiment then greatly reduces quantity and the ratio of broken string, has not had the broken string phenomenon fully.
Table two, the negative T line integrality statistical form that detects
Table three shows, in concentration be-pattern detection of 50%cut-off value in, the control experiment of two groups of test card 20 is set equally, standard is identical with above-mentioned negative sample test experience.Do not have improved test card also to have various " broken string " phenomenon, quantity and the ratio of detection line 106 broken strings seldom exist and have broken phenomenon when adopting test card 20 in the present embodiment then to greatly reduce concentration for-50%cut-off value.For example in BZO detects, do not have improved test card to compare with improved test card 20, the quantity of broken string between 1/2 to 1 is reduced to 17 from 97, and the broken string ratio is reduced to 14.2%, obvious broken string phenomenon and the outage that must improve product from 80.8%.
Table three ,-50%cut-off value detection T line integrality statistical form
Table four shows, in concentration be+pattern detection of 50%cut-off value in, the control experiment of two groups of test card 20 is set, standard is the same.Do not have improved test card 20 also to have various " broken string " phenomenon, quantity and the ratio of detection line 106 broken strings seldom exist and have broken phenomenon when adopting test card 20 in the present embodiment then to greatly reduce concentration for+50%cut-off value.For example in BZO detects, do not have improved test card to compare with improved test card 20, the quantity of broken string between 1/2 to 1 is reduced to 11 from 57, and the broken string ratio is reduced to 9.2%, obvious broken string phenomenon and the outage that must improve product from 47.5%.
Table four ,+50%cut-off value detection T line integrality statistical form
What present embodiment and embodiment 1 were different is, present embodiment provides a kind of test card 20, and this test card 20 can also comprise a lid.Lid can be used for and test card carrier 200 bottoms cooperate, and the sample region of acceptance 101 of detecting reagent strip 100 is enclosed in the lid, and like this, the liquid sample on the sample region of acceptance 101 does not pollute the environment or the testee.
Different with embodiment 2 is, present embodiment also comprises a supporter, and this supporter is positioned at the behind of test card 20, can make test card 20 erect convenient operation.
Embodiment 4
Different with embodiment 1 is that as shown in Figure 4 and Figure 5, the test card 20 in the present embodiment is placed in the test chamber 225 of a urine cup 2.This urine cup 2 comprises bowl cover 21, collection well 221, and plunger shaft 224 and test chamber 225 wherein can liquid communication between collection well 221 and the test chamber 225.Comprise lid holder 210 and caping 211 on the bowl cover 21, at caping 211 recess is arranged, can be used for depositing the push rod 212 of a circle.Test card 20 is by a carrier 200, detects reagent strip 100, and panel 232 and test chamber lid 231 form.Panel 232 is paperys, is used for the surface of carrier 200 is sealed, and prints a series of product informations etc.Test chamber lid 231 is transparent plastics, can cover on the outside of carrier 200.Plunger shaft 224 between collection well 221 and the test chamber 225, its effect is that the liquid rotating in the collection well 221 is moved on in the test chamber 225, plunger shaft 224 is equipped with piston 223, this piston 223 has primary importance and the second place, when piston 223 is positioned at primary importance, be obstructed between collection well 221 and the test chamber 225; When using push rod 212 pushing pistons 223, piston 223 can arrive the second place from primary importance, at this moment, is in the liquid communication state between collection well 221 and the test chamber 225, and can detects.
Embodiment 5
Present embodiment provides another kind of test card 30.Different with embodiment 1 is, such as Fig. 6, Fig. 7 and shown in Figure 8, comprise 5 draw-in grooves 301 on the board plug type test card carrier 30 as one kind 0 in the present embodiment, the width of draw-in groove 301 is greater than detecting 100 of reagent strips, be 5.4mm ± 0.2mm (width that wherein detects reagent strip 100 is 4mm) that draw-in groove is than detecting the wide 1.4mm ± 0.2mm of reagent strip.Distance is evenly separated by 4 fence 302 between the draw-in groove 301 between draw-in groove 301 and the draw-in groove 301.A rectangular aperture 303 is set on each draw-in groove 301, the position of opening 303 with detect reagent strip 100 on the position of marked region 102 corresponding.Two pairs of serrations 304 are set on each draw-in groove 301, can be used for clamping reagent strip 100, play the effect of stable detection reagent strip 100.Wherein a pair of serration 304 is positioned at the top of draw-in groove 301, and another is positioned at the bottom of draw-in groove 301 to serration 304.
Same, the test card in the present embodiment has been carried out the integrality of detection line 106 (T line) " broken string " and tested and sensitivity experiment.The result shows that the test card 30 of present embodiment also has the effect that improves preferably broken string.
1. sensitivity experiment
As shown in Table 5, select respectively 100 test card 30 as statistical experiment.The result shows, the standard that can reach G8 of all ' negative ' specimens; Most of concentration can reach the standard of G4 or G3.5, negative result for the-sample of 50%cut-off value after detection; Most of concentration also can reach the standard of G3, positive result for the+sample of 50%cut-off value after detection.The result of above-mentioned sensitivity experiment is reasonable, has all passed through to detect receptible scope.
Table five, the experiment of T line sensitivity
2. integrality experiment
In this experiment, comprise three groups of data, one group is broken string quantity and the outage (table six) of the testing result of negative sample, one group is that concentration is-the pattern detection result's of 50%cut-off value broken string quantity and outage (table seven), also has one group to be that concentration is+the pattern detection result's of 50%cut-off value broken string quantity and outage (table eight).
Table six shows, in the detection of negative sample, the control experiment of two groups of test card is set, and these two groups of test card are respectively the existing test card (U) of commonly using on the market and the improved test card 30 (I) according to the present invention.Its standard is with embodiment 1, there are three, respectively according to the performance level on the detection line 106, a standard is to exist greater than 1/2 on the detection line 106, bar less than 1, another standard is to exist greater than 1/4 on the detection line 106, the bar less than 1/2, and also having a standard is the bar that exists on the detection line 106 less than 1/4.Experimental result shows do not have improved test card also to have various " broken string " phenomenon, and the test card 30 in the employing present embodiment then greatly reduces quantity and the ratio of broken string, has not had the broken string phenomenon fully.
Table six, the negative T line integrality statistical form that detects
Table seven shows, in concentration be-pattern detection of 50%cut-off value in, the control experiment of two groups of test card is set, standard is the same.Do not have improved test card also to have various " broken string " phenomenon, quantity and the ratio of detection line 106 broken strings seldom exist and have broken phenomenon when adopting test card 30 in the present embodiment then to greatly reduce concentration for-50%cut-off value.For example in THC detects, do not have improved test card to compare with improved test card 30, the quantity of broken string between 1/2 to 1 is reduced to 61 from 95; In the detection of MDMA, the quantity of broken string between 1/2 to 1 is reduced to 29 from 73; In the detection of BUP, the quantity of broken string between 1/2 to 1 is reduced to 0 from 82.On the whole, more than the test card 30 obvious broken string phenomenons that must improve product in the present embodiment have been said in experiment, have reduced outage.
Table seven ,-50%cut-off value detection T line integrality statistical form
Table eight shows, in concentration be+pattern detection of 50%cut-off value in, the control experiment of two groups of test card is set, standard is the same.Do not have improved test card also to have various " broken string " phenomenon, quantity and the ratio of detection line 106 broken strings seldom exist and have broken phenomenon when adopting test card 30 in the present embodiment then to greatly reduce concentration for+50%cut-off value.For example in THC detects, do not have improved test card to compare with improved test card 30, the quantity of broken string between 1/2 to 1 is reduced to 10 from 52; In the detection of AMP, the quantity of broken string between 1/2 to 1 is reduced to 0 from 26; In the detection of OXY, the quantity of broken string between 1/2 to 1 is reduced to 2 from 43.On the whole, more than the test card 30 obvious broken string phenomenons that must improve product in the present embodiment have been said in experiment, have reduced outage.
Table eight ,+50%cut-off value detection T line integrality statistical form
Embodiment 6
Different with embodiment 5 is that as shown in Figure 9 and Figure 10, the test card 30 in the present embodiment is placed in the test chamber 322 of a urine cup 3.This urine cup 3 comprises bowl cover 31, cup 32 and test card 30.Screw thread 310 is arranged on the bowl cover 31, corresponding, also there are 323, two screw threads of screw thread to work in coordination in the cup, make collection well be in the state of sealing.Wherein, cup comprises collection well 321 and test chamber 322, and the bottom of collection well 321 and test chamber 322 communicates.Test card 30 can be inserted in the test chamber 322 of cup 32.A rubber strip 306 is arranged at the top of test card 30, can form at the opening of test chamber 322 cavity of a sealing.There is the heel 307 of strip at the back of test card 30, can be used for test card 30 is fixed on test chamber 322.In for detection of sample, whether contain in the analyte, collect first liquid sample to collection well 321, because test chamber 322 is airtight, liquid sample in collecting chamber 321, can only be by the thin channel of bottom between collection well 321 and the test chamber 322, make a part of sample carry out pattern delivery and transportation by the sample receiving pad 101 that detects reagent strip 100, reach the purpose of detection.Liquid flow in test chamber 322, because test card 30 is carried out such as the setting among the embodiment 5, can not produce capillarity and carry out liquid flow at test card plate carrier 30 as one kind 0 and the space of detecting between the reagent strip 100, can not carry out liquid flow in the behind of detecting reagent strip 100 and the space between the test card plate carrier 30 as one kind 0 yet.
Claims (6)
1. the carrier of a containing detection reagent strip, the draw-in groove that comprises supporting detection reagent strip, it is characterized in that, comprise on the described draw-in groove that blocking-up detects the structure that reagent strip liquid in addition flows at draw-in groove, wherein, described blocking-up structure comprises the opening that is positioned on the draw-in groove, and blocking-up detects the capillarity between reagent strip and the draw-in groove.
2. carrier as claimed in claim 1 is characterized in that, the corresponding at least marked region that detects reagent strip of described opening.
3. carrier as claimed in claim 2 is characterized in that, described draw-in groove also comprises makes the fixed fixed part of described detection reagent strip.
4. carrier as claimed in claim 3 is characterized in that, described fixed part is used for the suction zone of fixed test reagent strip.
5. carrier as claimed in claim 3 is characterized in that, described fixed part comprises the projection that detects reagent strip for clamping.
6. carrier as claimed in claim 1 is characterized in that, comprises on the described carrier that makes the upright supporter of carrier.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810120955 CN101354398B (en) | 2008-09-18 | 2008-09-18 | Carrier for containing detection reagent strip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810120955 CN101354398B (en) | 2008-09-18 | 2008-09-18 | Carrier for containing detection reagent strip |
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| CN101354398A CN101354398A (en) | 2009-01-28 |
| CN101354398B true CN101354398B (en) | 2013-02-27 |
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| CN 200810120955 Active CN101354398B (en) | 2008-09-18 | 2008-09-18 | Carrier for containing detection reagent strip |
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| CN111443205A (en) * | 2020-02-25 | 2020-07-24 | 古镜科技(深圳)有限公司 | Quintuplet test paper for detecting abuse of mental drugs and preparation thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0183442B1 (en) * | 1984-11-15 | 1990-03-21 | Syntex (U.S.A.) Inc. | Chromatographic device and method |
| CN201273897Y (en) * | 2008-09-18 | 2009-07-15 | 艾博生物医药(杭州)有限公司 | Carrier for supporting detection reagent strip |
| CN201421446Y (en) * | 2008-09-18 | 2010-03-10 | 艾博生物医药(杭州)有限公司 | Carrier for supporting test reagent strip |
-
2008
- 2008-09-18 CN CN 200810120955 patent/CN101354398B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0183442B1 (en) * | 1984-11-15 | 1990-03-21 | Syntex (U.S.A.) Inc. | Chromatographic device and method |
| CN201273897Y (en) * | 2008-09-18 | 2009-07-15 | 艾博生物医药(杭州)有限公司 | Carrier for supporting detection reagent strip |
| CN201421446Y (en) * | 2008-09-18 | 2010-03-10 | 艾博生物医药(杭州)有限公司 | Carrier for supporting test reagent strip |
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