CN101229531B - Combined type centrifugal tube containing U trap valve - Google Patents
Combined type centrifugal tube containing U trap valve Download PDFInfo
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- CN101229531B CN101229531B CN2007100025140A CN200710002514A CN101229531B CN 101229531 B CN101229531 B CN 101229531B CN 2007100025140 A CN2007100025140 A CN 2007100025140A CN 200710002514 A CN200710002514 A CN 200710002514A CN 101229531 B CN101229531 B CN 101229531B
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- filter screen
- centrifuge tube
- type filter
- filter membrane
- stainless steel
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Abstract
The invention relates to a combined type centrifuge tube with a U-shaped filter screen. When honeybee magnetic nano-granular purification is carried out, the invention can be used for separating pollen from honeybee abdominal and separating small-particle pollutants absorbed on cilia, iron deposition cells and iron particles from the honeybee abdominal. The filter screen of the centrifuge tube is a U-shaped stainless steel filter screen or a U-shaped plastic filter screen. The U-shaped arc of the filter screen can increase the separation surface of the filter screen; sample can be completely soaked in buffer solution to be separated so as to provide and quickly separate a great deal of impurities. The combination method of the filter screen and the centrifuge tube is that a support frame of the U-shaped filter is embedded in bottom mouth of the centrifuge tube; the centrifuge tube and the filter screen can be randomly assembled according to volume and mesh diameter of the filter screen.
Description
Technical field
The invention provides a kind of centrifuge tube of centrifuge and the filter screen of centrifuge tube use, refer to a kind of combined centrifugal pipe that contains U type filter screen of removing impurities in a large number especially.
Background technology
No. the 6221655th, United States Patent (USP), main at isolated protein or littler molecule, and the present invention is used for a large amount of removing impurities, quickens to remove the material of impurity to obtain to desire to take, in order to further analysis.The impurity that desire is removed can be tissue, cell and the above material of born of the same parents' device level (containing) according to adopting the decision of filter screen pore size.The material size of desiring to take can be tissue, cell and the following material of born of the same parents' device level according to adopting the decision of filter screen pore size.The filter membrane of United States Patent (USP) is placed on the bottom of tubing string, with the plastic cement shore supports, non-integrally formed filter membrane, filter membrane is placed on and places another tubing string in the tubing string again, non-filter screen is placed directly in the opening part of centrifuge tube, assembling mode difference, non-stainless steel of filter screen material and plastic cement, filter screen is not capable of washing, and not reproducible use.The present invention forms screen holder with integrally formed filter screen, is placed on the opening part of centrifuge tube, and therefore easy to assembly, the filter screen material is stainless steel and plastic cement.Filter screen mainly separates greater than the impurity of cell grade such as cilium, chitin fragment, animal tissue's fragment, plant tissue fragment and the grains of sand etc., screen holder capable of washing and repeated use and cleaning convenience.When carrying out purifying, can use the present invention earlier usually, removing the above material of cell grade (containing), unless and be necessary, carry out follow-up Separation of Proteins just must re-use the United States Patent (USP) case.
When carrying out honeybee nano magnetic particle purifying, after the honeybee dissection, keep and remove gastral belly, go gastral belly need utilize special centrifuge tube, could effectively separate the honeybee belly and on pollen, honeybee belly and adsorb small particle contamination thing and deposition of iron cell and iron particle on its cilium.It is available at present still not have suitable centrifuge tube on the market, causes honeybee nano magnetic particle purification technique can't break through always.
Be with, at the existing problem points of above-mentioned known person, how to develop a kind of innovation structure that has more desirable practicality, the ardent hope of real purification technique person institute also is target and the direction that relevant dealer must make great efforts the research and development breakthrough.
In view of this, the inventor this in manufacturing development and the design experiences of being engaged in Related product for many years, at above-mentioned target, in detail after design and the scrupulous assessment, eventually the present invention of a true tool practicality.
Summary of the invention
The invention provides a kind of combined centrifugal pipe of the U of containing type filter screen, known when carrying out honeybee nano magnetic particle purifying to solve, after the honeybee dissection, keep and remove gastral belly, go gastral belly need utilize special centrifuge tube, could effectively separate the honeybee belly and on pollen, honeybee belly and adsorb small particle contamination thing on its cilium, with deposition of iron cell and iron particle, and it is available still not have suitable centrifuge tube at present on the market, the problem that causes honeybee nano magnetic particle purification technique always can't break through.
The technical characterstic of dealing with problems: the combined centrifugal pipe that a kind of U of containing type filter screen is provided, mainly when carrying out honeybee nano magnetic particle purifying, separate the honeybee belly and on pollen, honeybee belly and adsorb small particle contamination thing and deposition of iron cell and iron particle on its cilium.The filter screen of this centrifuge tube is U type stainless steel filtering net or U shape glue filter screen.The U type radian of filter screen can increase the surface area that filter screen separates, and sample can be fully immersed in the cushioning liquid and separate, and a large amount of and quick removing impurities are provided according to this.The combination of filter screen and centrifuge tube is that U type filter screen is embedded in the centrifuge tube bottle mouth position, can need arbitrarily assembling centrifuge tube and U type filter screen according to volume and filter screen pore size.
The effect of contrast prior art: provide a kind of confession to be used in the special U type filter screen and the centrifuge tube of honeybee nano magnetic particle purification technique, the surface area that the filter screen that is increased separates, and sample can be fully immersed in the cushioning liquid and separate, and a large amount of and quick removing impurities person are provided.
The technology that relevant the present invention adopted, means and effect thereof after lifting a preferred embodiment now and cooperating graphic being described in more detail in, are believed the above-mentioned purpose of the present invention, structure and feature, when can by one deeply and concrete understanding.
Description of drawings
Fig. 1: be embodiment of the invention schematic perspective view.
Fig. 2: be embodiment of the invention perspective exploded view.
Fig. 3: be embodiment of the invention plane cut-away view.
The specific embodiment
Consult Fig. 1, shown in Figure 2, the invention provides a kind of combined centrifugal pipe of the U of containing type filter screen, the bottleneck 11 that refers in particular to this centrifuge tube 10 forms in conjunction with a U type stainless steel filtering net 20, mainly utilize the stainless steel filtering net of 40mesh to make the U type filter screen 20 with degree of depth, U type filter screen 20 includes the filter screen fringing and supports frame 21 to form screen holder.
Occupation mode is: this U type filter screen is placed on the opening part of 400ml centrifuge tube 10 bottlenecks 11, filter screen 20 edges need with bottleneck 11 driving fits, pin with bottle cap 30, with to centrifugal force resistant.Afterwards, it is centrifugal to put into centrifuge, 3000g 10 minutes.At this moment, manage centrifuge tube 10 entirely and all contain cushioning liquid, honeybee removes gastral belly, can Shen float in the cushioning liquid on the filter screen 20, because filter screen 20 is U types, so, the surface area maximum that filter screen 20 can be used.Remove gastral belly, separate, when centrifugal, can exist in the filter screen 20 because of filter screen 20 is arranged, be attached on the honeybee belly and on pollen, honeybee belly and adsorb small particle contamination thing on its cilium because of being subjected to centrifugal force, can be deposited on centrifuge tube 10 bottoms by filter membrane.When centrifugation time then, bottle cap 30 is opened, gentle agitation is removed gastral belly, go gastral belly can Shen to float in the cushioning liquid on the filter screen 20, centrifugal again as above-mentioned step, so carry out three times, with remove the honeybee belly and on pollen, honeybee belly and adsorb small particle contamination thing on its cilium.
Consult shown in Figure 3ly, behind the long-pending cell in extruding iron Shen, need to use the centrifuge tube 10 that includes U type stainless steel filtering net 20, to separate the long-pending cell in epidermis and iron Shen.Utilize the stainless steel filtering net of 200mesh to make U type filter screen 20 and be placed on 50ml centrifuge tube 10 bottlenecks 11 opening parts, filter screen 20 edges need with bottleneck 11 driving fits, pin with bottle cap 30, with to centrifugal force resistant.Afterwards, it is centrifugal to put into centrifuge, 15000g 10 minutes.At this moment, manage centrifuge tube 10 entirely and all contain cushioning liquid, the honeybee belly that pushed can Shen floats in the cushioning liquid on the filter screen 20, because filter screen 20 is U types, so, the surface area maximum that filter screen 20 can be used.The belly that pushed is isolated because of filter screen 20 is arranged, and can exist in the filter screen 20 when centrifugal, and the long-pending cell in iron Shen that is attached on belly can be deposited on centrifuge tube 10 bottoms by filter screen 20 because of being subjected to centrifugal force.When centrifugation time then, bottle cap 30 is opened, the belly that gentle agitation was pushed, the belly that pushed can Shen in the cushioning liquid on filter screen 20, and be centrifugal again as above-mentioned step, so carries out three times, to obtain the long-pending cell in iron Shen.
After breaking the long-pending cell in iron Shen, need to use the 17ml centrifuge tube that includes 10 μ m U shape glue filter membranes, with identical method removing impurities and iron particle.
These combined centrifugal Guan Weijian that include U type filter screen have sale on the market, tool novelty of the present invention and progressive.Because can using volume respectively, the pore size demand of the U type filter screen that uses is 17ml, the centrifuge tube of 50ml or 400ml again.After the commercialization volume production, can select for use different U type filter screen and centrifuge tube to arrange in pairs or groups mutually according to required filter screen pore size and volume size, with more convenient use.
The present invention's characteristics are:
A kind of combined centrifugal pipe that contains U type filter screen of the present invention, it includes:
One U type filter membrane filter screen, this filter screen are provided with U moldeed depth degree film wire side, and the filter screen opening part has more filter cloth edge;
One centrifuge tube, this centrifuge tube bottleneck is provided with a bottle edge;
One bottle cap, this bottle cap is locked in the centrifuge tube bottleneck;
This U type filter membrane stainless steel filtering net group is gone in the centrifuge tube, and filter cloth edge need with the driving fit of bottle edge, when pinning centrifuge tube with bottle cap again, the bottle cap inner face is tightly suppressed filter cloth edge with a bottle edge and is fixed.
Described U type filter membrane filter screen is the plastic cement filter screen.
Described U type filter membrane filter screen is a stainless steel filtering net.
Described U type filter membrane stainless steel filtering net can be the stainless steel filtering net of 40mesh.
Described U type filter membrane stainless steel filtering net can be the stainless steel filtering net of 200mesh.
Described U type filter membrane stainless steel filtering net can be the stainless steel filtering net of 100mesh.
Described U type filter membrane filter screen can be the plastic cement filter membrane of 10 μ m.
Before system carry out concrete explanation at the present invention's preferred embodiment for the present invention's technical characterictic; Only, the personage who is familiar with this technology is when can changing and revise the present invention under the spirit that does not break away from the present invention and principle, and these changes and modification, all should be covered by in the category that the application's claim defined.
Claims (5)
1. combined centrifugal pipe that contains U type filter screen includes:
One U type filter membrane filter screen, this filter screen are provided with U moldeed depth degree film wire side, and filter membrane filter screen opening part has the support frame, and this filter membrane filter screen is stainless steel filtering net or plastic cement filter membrane;
One centrifuge tube, this centrifuge tube bottleneck is provided with a bottle edge;
One bottle cap, this bottle cap is locked in the centrifuge tube bottleneck;
This U type filter membrane filter screen group is gone in the centrifuge tube, and the filter membrane filter screen support frame need with the driving fit of bottle edge, when pinning centrifuge tube with bottle cap again, the bottle cap inner face is tightly suppressed the filter membrane filter screen with a bottle edge and supports frame to fix.
2. contain the combined centrifugal pipe of U type filter screen according to claim 1, it is characterized in that, this U type filter membrane filter screen is the stainless steel filtering net of 40mesh.
3. contain the combined centrifugal pipe of U type filter screen according to claim 1, it is characterized in that, this U type filter membrane filter screen is the stainless steel filtering net of 200mesh.
4. contain the combined centrifugal pipe of U type filter screen according to claim 1, it is characterized in that, this U type filter membrane filter screen is the stainless steel filtering net of 100mesh.
5. contain the combined centrifugal pipe of U type filter screen according to claim 1, it is characterized in that, this U type filter membrane filter screen is 10 μ m plastic cement filter screens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100025140A CN101229531B (en) | 2007-01-25 | 2007-01-25 | Combined type centrifugal tube containing U trap valve |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100025140A CN101229531B (en) | 2007-01-25 | 2007-01-25 | Combined type centrifugal tube containing U trap valve |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101229531A CN101229531A (en) | 2008-07-30 |
| CN101229531B true CN101229531B (en) | 2010-08-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100025140A Expired - Fee Related CN101229531B (en) | 2007-01-25 | 2007-01-25 | Combined type centrifugal tube containing U trap valve |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104812421B (en) * | 2012-08-15 | 2017-06-06 | 旋风医疗科技股份有限公司 | For the system and method for the Blood calldack from absorbable surgical material |
| CN105136552B (en) * | 2015-10-15 | 2018-09-14 | 北京农学院 | A kind of device and method extracting apoplast juice from plant fruit tissue |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4472276A (en) * | 1980-07-24 | 1984-09-18 | The Regents Of The University Of California | High-speed centrifugal filter assembly and method |
| CN86200451U (en) * | 1986-01-27 | 1987-02-18 | 梁殿栋 | Cyclone purifier |
| CN1061920A (en) * | 1991-11-11 | 1992-06-17 | 朱学良 | Vertical centrifuge with special shaped net-wall de-fluid drum |
| CN2176817Y (en) * | 1993-03-14 | 1994-09-14 | 安金城 | Centrifugal filter |
| CN2181337Y (en) * | 1994-01-19 | 1994-11-02 | 华西医科大学药学院 | Small centrifugal filter |
| US6221655B1 (en) * | 1998-08-01 | 2001-04-24 | Cytosignal | Spin filter assembly for isolation and analysis |
-
2007
- 2007-01-25 CN CN2007100025140A patent/CN101229531B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4472276A (en) * | 1980-07-24 | 1984-09-18 | The Regents Of The University Of California | High-speed centrifugal filter assembly and method |
| CN86200451U (en) * | 1986-01-27 | 1987-02-18 | 梁殿栋 | Cyclone purifier |
| CN1061920A (en) * | 1991-11-11 | 1992-06-17 | 朱学良 | Vertical centrifuge with special shaped net-wall de-fluid drum |
| CN2176817Y (en) * | 1993-03-14 | 1994-09-14 | 安金城 | Centrifugal filter |
| CN2181337Y (en) * | 1994-01-19 | 1994-11-02 | 华西医科大学药学院 | Small centrifugal filter |
| US6221655B1 (en) * | 1998-08-01 | 2001-04-24 | Cytosignal | Spin filter assembly for isolation and analysis |
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| Publication number | Publication date |
|---|---|
| CN101229531A (en) | 2008-07-30 |
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Granted publication date: 20100811 Termination date: 20120125 |