CN101109761B

CN101109761B - Fluidic device and control method thereof

Info

Publication number: CN101109761B
Application number: CN2006101495740A
Authority: CN
Inventors: 翁国曜
Original assignee: Industrial Technology Research Institute ITRI
Current assignee: Industrial Technology Research Institute ITRI
Priority date: 2006-07-17
Filing date: 2006-11-21
Publication date: 2012-05-30
Anticipated expiration: 2026-11-21
Also published as: TWI338081B; CN101109761A; TW200813324A; TW201000901A; TWI404929B; TWI336781B; CN101109759B; CN101109759A; TW200951436A; TW200806988A

Abstract

A fluidic device includes a first material defining a first region, a second material defining a second region that is separated from the first region, and a connector coupled between the first region and the second region. The connector includes a brittle material and has an open end and a closed end, the open end being disposed in the second region, the closed end being disposed in the first region. The first region is closed off from the second region by the closed end of the connector. The connector is configured such that when the closed end of the connector is broken, the connector defines a passage from the first region to the second region.

Description

Fluid means and control method thereof

Technical field

The present invention relates to a kind of fluid means and control method thereof.

Background technology

Can use the proving installation of many types to detect the existence or the analysis of biochemical reaction of compound.For example, can use the cross-current film (Lateral flow membrane) that has one or more p-wire along its length to carry out cross-current calibrating (Lateral Flow Assays).Fluid with reagent of dissolving marches to p-wire through electro-osmosis (Electro osmosis) from an end of film.Read taker detecting p-wire place and whether react, have or do not exist some particle in its indicator.As another instance, the fluid that can use the device with one group of microscopic capillary to control in the immunoassays processing flows.Reagent is positioned a plurality of positions along the length of microscopic capillary, makes that fluid contacts with reagent when fluid flows in microscopic capillary owing to capillary force.Whether read the site that taker monitoring reagent is positioned at reacts to judge.As another instance, flow through the flow through fluid of a plurality of passages and chamber of control, can use micro-fluid chip to carry out calibrating.Can be with providing external power source and/or pump to use micro-fluid chip in order to the driving force of drive fluid.

Summary of the invention

The present invention proposes a kind of fluid means that is used to carry out calibrating; Comprise the Control Component such as vacuum pump, gas pump, " breakaway-element is opened valve (Broken open valves) " and " self-closing valve (self-close valves) ", its fluid that is used for controlling fluid means flows.

Carry out the fluid means of calibrating in proposed by the invention being used to, can use vacuum pump along the fluid in the specific direction traction passage, but and the using gases pump come to promote the fluid in the passage along specific direction.

Carry out the fluid means of calibrating in proposed by the invention being used to, can use breakaway-element to open valve and connect two isolated areas by user's control, and can use self-closing valve at fluid through seal channel automatically later.

Carry out the fluid means of calibrating in proposed by the invention being used to, vacuum pump, gas pump, breakaway-element open valve and self-closing valve can manufacture the very little element of volume, makes fluid means can be fabricated to the very little and portable device of volume.

The present invention provides a kind of method of controlling fluid, flows in passage in order to the control fluid.The method comprises breaks off first container in passage, to produce pressure differential, make fluid in said passage, move, and first container is processed by hard brittle material.This first container (a) defines the space in first container; The space has the gaseous tension of the gaseous tension that is different from first external container; Or (b) comprise one first material; This first material is separated with one second material that is positioned at first external container before first container breaks off, and first material and second material through selecting so that after first material and the interaction of second material, produce gas immediately.

The present invention provides a kind of fluid means, comprises the passage and first container.In first container, when first container broke off, first container produced pressure differential in passage.And first container is processed by hard brittle material.And this first container (a) defines the space in first container; This space has the gaseous tension of the gaseous tension that is different from first external container; Or (b) comprise one first material; This first material is separated with one second material that is positioned at first external container before first container breaks off, this first material and second material through selecting so that produce gas immediately after first material and the interaction of second material.

The present invention provides a kind of fluid means, comprises first material that defines the first area and second material that defines second area, and this second area and first area are separated.And comprising that more connector is coupled between first area and the second area, this connector comprises hard brittle material and has the open end and closing end.This open end is placed in the second area, and closing end is placed in the first area.The first area cuts off through the closing end and the second area of connector.Connector through setting so that when the closing end disconnection of said connector, connector defines from the first area to the path of second area.

The present invention provides a kind of fluid means, comprises the passage of dilation, and this dilation has the diameter greater than the adjacent part of passage, and more comprises the material that is placed in the dilation.This material has before fluid-absorbent the not volume of the path of block fluid.Wherein volume expands so that expand the path of material blocking-up additional fluid in back through passage immediately after the part of this material fluid-absorbent.And this material comprises super absorbent polymer.

The present invention provides a kind of fluid means, comprises first reservoir that contains first fluid, second reservoir that contains second fluid, main channel, first branched bottom, second branched bottom, first single use pump and second single and uses pump.This first branched bottom is couple to first reservoir to the main channel.And second branched bottom is couple to second reservoir to the main channel.This first single uses pump then when first single uses container in the pump to break off, and this first single uses pump to produce pressure differential with one in the mobile first fluid and second fluid or both.And when second single used container in the pump to break off, second single used pump to produce pressure differential to move one in the first fluid and second fluid or both.

The present invention provides a kind of method of controlling fluid, and comprising provides a plurality of suction pipes so that the fluid of the scheduled volume of can taking a sample.And each suction pipe comprises passage, and container, and this container produces pressure differential when breaking off in passage, and this container is processed by hard brittle material.This container defines the space in the container, and this space has the gaseous tension less than the gaseous tension of external container, and it breaks container can produce scheduled volume in passage pressure differential, so that the fluid of scheduled volume is drawn to passage.

The present invention provides a kind of method of controlling fluid; Comprise and make fluid to flow to second area from the first area; Wherein, This first area is coupled to second area through the connector with open end and closing end, and this open end is placed in the second area, and this closing end is placed in the first area.The closing end of this first area through connector cuts off with second area, wherein makes fluid to flow to comprise the closing end of disconnect connector, with form pass through connector from the first area to the path of second area.Through using the material of volumetric expansion after the fluid-absorbent, the part of adsorbing the fluid of the connector of flowing through, and use through the material that expands and block the additional fluid connector of further flowing through.

The present invention provides a kind of method of controlling fluid; Comprise fluid is flowed in passage; And this passage comprises the material that volume expands immediately after the part of fluid-absorbent; Fluid is flowed comprise flow through material and use said material to come the second portion of fluid-absorbent of the first that makes fluid in passage, thereby make material volume expand.And block the path of additional fluid through passage through using through the material that expands.

The present invention provides a kind of method of controlling fluid, comprises to make fluid through passage, and this passage comprises first self-closing valve and second self-closing valve.This first self-closing valve and second self-closing valve are spaced apart from each other, and each self-closing valve comprises the fluid adsorption material that volume expands immediately after the part of fluid-absorbent.Through using fluid adsorption material in first self-closing valve and second self-closing valve to come the part of fluid-absorbent.The volume of expansion fluid sorbing material further passes through the path of passage with the blocking-up additional fluid, the fluid of maintenance scheduled volume in first self-closing valve of passage and the part between second self-closing valve.

The present invention provides a kind of method of controlling fluid, comprises first container that disconnection processed by hard brittle material in passage, to produce pressure differential, makes first fluid move to first section of passage from first reservoir.This first container (a) defines the space in first container; This space has the gaseous tension of the gaseous tension that is different from first external container; Or (b) comprise first material; This first material is separated with second material before first container breaks off, this first material and this second material through selecting so that produce gas immediately after first material and the interaction of second material.Second container that disconnection is processed by hard brittle material in passage, to produce pressure differential, makes the part at least of first fluid move and second section of process passage.

The present invention provides a kind of method of controlling fluid, comprise to operate first single simultaneously and use the pump and second single to use pump, with first to the first passage of drawing sample fluid and the second portion of drawing sample fluid to second channel.Break off first single and use first container in the pump; To produce pressure differential; Make the first of sample fluid move to first passage from reservoir; And break off second single and use second container in the pump, make the second portion of sample fluid move to second channel from reservoir to produce pressure differential.Operating the 3rd single simultaneously uses pump and the 4th single to use pump to draw first buffer solution to first passage and to draw second buffer solution to second channel.

Description of drawings

Figure 1A and Figure 1B are the synoptic diagram of vacuum pump;

Fig. 2 A and Fig. 2 B are the synoptic diagram of gas pump;

Fig. 3 A and Fig. 3 B are the synoptic diagram of gas pump;

Fig. 4 A is the synoptic diagram of gas pump;

Fig. 4 B is the table of material;

Fig. 5 A and Fig. 5 B are the synoptic diagram that breakaway-element is opened valve;

Fig. 6 A, Fig. 6 B, Fig. 7 A, Fig. 7 B and Fig. 8 A to Fig. 8 C are the synoptic diagram of self-closing valve;

Fig. 9 A to Fig. 9 C is the synoptic diagram that valve left by switch;

Figure 10 A to Figure 10 C is for closing the synoptic diagram of switch valve;

Figure 11 A to Figure 11 D is the synoptic diagram of switch switch valve;

Figure 12 is the synoptic diagram of measuring pipet(te);

Figure 13 is the synoptic diagram of measuring pipet(te);

Figure 14 A to Figure 14 C is the synoptic diagram of measuring pipet(te);

Figure 15 A and Figure 15 B are the synoptic diagram of WT-MSR;

Figure 16 A and Figure 16 B are the synoptic diagram of WT-MSR;

Figure 17 A to Figure 17 C is the schematic representation of apparatus that is used for the calibrating of two steps;

Figure 18 A to Figure 18 C is the schematic representation of apparatus that is used for the calibrating of two steps;

Figure 19 A to Figure 19 C is the schematic representation of apparatus that is used for the calibrating of three steps;

Figure 20 is the synoptic diagram that is used for the module of multiple analysis assay;

Figure 21 A and Figure 21 B show and are used for the take a sample measuring pipet(te) of blood from the patient;

Figure 22 A and Figure 22 B are the schematic representation of apparatus that is used to carry out the calibrating of rapid reaction colorimetric;

Figure 23 A and Figure 23 B are used to take a sample schematic representation of apparatus through the fluid that filters;

Figure 24 A to Figure 24 C is the schematic representation of apparatus that is used to carry out slow colorimetric calibrating;

Figure 25 A to Figure 25 C is the synoptic diagram of vacuum pump;

Figure 26 A and Figure 26 B are the synoptic diagram of vacuum pump;

Figure 27 A to Figure 27 C is the synoptic diagram of self-closing valve;

Figure 28 A and Figure 28 B are the synoptic diagram that breakaway-element is opened valve;

Figure 28 C shows the xsect of the glass capillary with square inside circumference and neighboring;

The combination manufacturing of passing through to use self-closing valve (SLV) and breakaway-element to open valve (BOV) of Figure 29 A and the 29B explanation specific embodiment of the invention is used for blood sample is carried out the device that antibody (Antibody) is examined and determine.

Figure 30 A, Figure 30 B and Figure 30 C key diagram 29A carry out antibody calibrating installation operation chart to blood sample;

Figure 31 explains that the combination manufacturing of passing through to use self-closing valve (SLV) and breakaway-element to open valve (BOV) of another specific embodiment of the present invention is used for blood sample is carried out the device that antibody is examined and determine.

The main element symbol description:

90: vacuum pump

92: gas pump

94: gas pump

96: gas pump

100: container

102: material

104: the zone

106: passage

108: direction

110: container

112: the zone

114: direction

120: glass capillary

124: passage

126: the first materials

128: the second materials

130: compound

132: glass capillary

140: breakaway-element is opened valve

142: glass capillary

144: the open end

146: closing end

148: first passage

150: second channel

152: second channel

160: self-closing valve

162: super absorbent polymer (SAP)

164: passage

166: the first area

168: second area

170: self-closing valve

172: enlargement steps down

180: lead

182: pocket regions

190: valve left by switch

210: the switch switch valve

212: super absorbent polymer (SAP)

214: super absorbent polymer (SAP)

220: measuring pipet(te)

222: measuring pipet(te)

224: the suction pipe body

226: spheroid

228: body

230: suction pipe

232: gas pump

234: the suction pipe spheroid

240: measuring pipet(te)

242: spheroid

244: center section

246: the suction pipe body

248: the suction pipe body

260: WT-MSR

262: glass capillary

264: section

266a: branch

266b: branch

268a: self-closing valve

268b: self-closing valve

270a: breakaway-element is opened valve

270b: breakaway-element is opened valve

274: fluid

280: WT-MSR

282: sample pool

284a: kapillary

284b: kapillary

284c: kapillary

286a: self-closing valve

286b: self-closing valve

286c: self-closing valve

288a: vacuum valve

288b: vacuum valve

288c: vacuum valve

290: device

292a: first vacuum pump

292b: second vacuum pump

292c: the 3rd vacuum pump

294: breakaway-element is opened valve

296: self-closing valve

298: damping fluid

300: sample pool

302: passage

304: passage

306: combine and/or sensing area

308: passage

310: device

314: gas pump

320: device

322: passage

324: the second damping fluids

326: breakaway-element is opened valve

330: module

332a: chamber

332b: chamber

332c: chamber

334a: vacuum pump

336a: second vacuum pump

338a: the 3rd vacuum pump

340a: the 4th vacuum pump

342a: passage

344a: self-closing valve

346a: self-closing valve

348a: breakaway-element is opened valve

350a: first damping fluid

352a: self-closing valve

354a: breakaway-element is opened valve

356a: second damping fluid

358a: self-closing valve

360a: breakaway-element is opened valve

362a: the 3rd damping fluid

370: blood

380: device

382: sample pool

384: passage

386: the test section

390: device

392: filtration membrane

400: device

402: sample pool

404: gas pump

406: sample fluid

408: band

410: gas pump

412: gas pump

416: the vacuum glass kapillary

418: pipe

420: proofed sleeve

424: closing end

426: the open end

428: closing end

430: vacuum pump

432: vacuum pump

434: planar substrates

436: elastic layer

438: the fluid passage

440: closing end

442: the open end

444: the fluid passage

450: lead

460: plastic of conduit

462: plastic of conduit

464: reactant

The 466:UV light source

468: detector

500: the device that is used for blood sample is carried out the antibody calibrating

501; Blood sample pond (Blood sample well)

503: dcq buffer liquid pool (Washing buffer well)

505: metering region and sign antibody (Metering zone and labeled antibody) zone

507: diagnosis zone (Diganostic Zone) (antibody array Antibody array)

509: breakaway-element is opened valve

511: discarded object pond (Waste well)

513: self-closing valve (SLV)

Embodiment

A kind of fluid means that is used to carry out calibrating can comprise the Control Component such as vacuum pump, gas pump, " breakaway-element is opened valve (Broken open valves) " and " self-closing valve (self-close valves) ", and its fluid that is used for controlling fluid means flows.Can use vacuum pump along the fluid in the specific direction traction passage, but and the using gases pump come to promote the fluid in the passage along specific direction.Can use breakaway-element to open valve and connect two isolated areas, and can use self-closing valve at the later automatic seal channel of fluid warp by user's control.Vacuum pump, gas pump, breakaway-element are opened valve and self-closing valve and can be fabricated to for a short time, make fluid means can be fabricated to little and for portable.

In the following description, can at first introduce indivedual Control Components, and how be used for controlling the description of modular unit of the fluid of fluid means with construction for Control Component subsequently through combination.To describe subsequently and how use fluid means to carry out biological calibrating.

Referring to Figure 1A, can come construction vacuum pump 90 through container 100 being placed in the path 10 6 (or chamber) that defines by material 102.Container 100 closed regions 104, zone 104 for vacuum or have with path 10 6 in the gaseous tension low gaseous tension of comparing.

Referring to Figure 1B, container 100 can be (for example) glass capillary, and it breaks off after applying external force immediately.When container 100 broke off, the gas in the path 10 6 flowed in the vacuum area 104, thereby reduced the pressure in the zone 106.Producing in this way can be along direction 108 draw fluid so that its attractive force towards zone 106.

Figure 25 A to Figure 25 C shows the instance that uses the vacuum pump that is placed in the glass capillary in the proofed sleeve.Figure 25 A shows the xsect of gas pump 410, and gas pump 410 has the vacuum glass kapillary 416 that is placed in the proofed sleeve 418, wherein manages 418 and has closing end 424 and open end 426.Figure 25 B shows the xsect of gas pump 412, and gas pump 412 is similar to gas pump 410, has the proofed sleeve 420 of two open ends except having one.Figure 25 C shows the gas pump 412 that is connected to two proofed sleeves 428, and wherein proofed sleeve 420 has than the internal diameter (to hold glass capillary 416) greater than proofed sleeve 428.

Figure 26 A and Figure 26 B show the instance that uses the vacuum pump that is placed in the glass capillary in the fluid passage, plane.Figure 26 A shows the xsect of vacuum pump 430, and vacuum pump 430 has the vacuum glass kapillary 416 that is placed in the fluid passage of being defined by planar substrates 434 438.Fluid passage 438 has closing end 440 and open end 442.Planar substrates 434 is processed by rigid material.Elastic layer 436 is embedded in the position of adjacent capillaries 416 in the substrate 434, makes to allow the user to apply external force to break off kapillary 416 via elastic layer.

Figure 26 B shows the xsect of vacuum pump 432, and vacuum pump 432 is similar to vacuum pump 430, except fluid passage 438 is connected to two fluid passages 444 with smaller cross-sectional area.

Can form first closure with melten glass through a heating glass end capillaceous brings in and processes the vacuum glass kapillary.Use vacuum pump to go out the air in the glass capillary via the open end pumping.At distance first closing end one distance heating glass kapillary.The heat soften glass can be pinched and contract or reverse through softening glass to form second closing end.

Referring to Fig. 2 A, can come construction gas pump 92 through container 110 being placed in the path 10 6 (or chamber) that defines by material 102.Container 110 closed regions 112, zone 112 have with the outside path 10 6 of container 110 in gaseous tension compare higher gaseous tension.

Referring to Fig. 2 B, container 110 can be (for example) glass capillary, and it breaks off after applying external force immediately.When container 110 breaks off,, thereby increased the pressure in the zone 106 originally at container 110 gas inside flow containers 110.Producing in this way can be along direction 114 propelling fluids so that its power away from zone 106.

In this described, will use a technical term " vacuum pump " made a general reference a kind of device that produces tractive force, and it can be in order to towards the device draw fluid; And will use a technical term " gas pump " made a general reference a kind of device that produces expulsive force, and it can be in order to propelling fluid so that it be away from device.

Existence is in order to the alternative of construction gas pump.For example, referring to Fig. 3 A, can make gas pump 94 through the partially filled glass capillary 120 that first material 126 arranged is placed in the passage 124 (or chamber) that contains second material 128.Select first material 126 and second material 128, make when material 126 and material 128 are mixed with each other, will interact and produce one or more gas.For example, first material 126 can be sodium carbonate (Na ₂CO ₃) and/or soda mint (NaHCO ₃), and second material 128 can be acetate (CH ₂COOH).

Referring to Fig. 3 B, when applying external force with disconnection glass capillary 120, first material 126 and second material 128 interact and produce gas.In this example, gas is carbon dioxide (CO ₂).The chemical reaction that takes place is:

Na ₂CO ₃+2CH ₂COOH→2NaCOOCH ₂+H ₂O+CO ₂

NaHCO ₃+CH ₂COOH→NaCOOCH ₂+H ₂O+CO ₂

Carbon dioxide increases the pressure in the passage 124, can be in order to the power of propelling fluid away from the kapillary 120 that breaks off thereby produce.

First material 126 can directly be filled in the kapillary 120.Referring to Figure 27 A, first material 126 also can be attached to lead 450, and lead 450 is placed in kapillary 120 inside with coating material 126 subsequently.Figure 27 B displaying is placed in the instance in the passage 124 in the proofed sleeve 418 with glass capillary 120.Passage 124 contains second material 128, and second material 128 can interact with first material 126 when glass capillary 120 breaks off.Figure 27 C displaying is placed in the instance in the fluid passage 438 in the plane device substrate 434 with glass capillary 120.Elastic layer 436 is embedded in the position of adjacent capillaries 120 in the substrate 434, makes to allow the user to apply external force to break off kapillary 120 via elastic layer 436.

Referring to Fig. 4 A, can make gas pump 96 through following steps: with compound 130 be placed in the glass capillary 132, sealed hair tubule 132, heated capillary 132, cooling kapillary 132 and kapillary 132 is placed in the path 10 6 (or chamber).Compound 130 is chosen as the material that after heating, produces gas.When heating and cooling kapillary 132, the gas that is produced by compound 130 increases kapillary 132 gas inside pressure (comparing with kapillary 132 outside gaseous tensions).

The instance of compound 130 comprises soda mint (NaHCO ₃) and lime carbonate (CaCO ₃).Produce carbon dioxide during these compound heating:

NaHCO ₃→NaOH+CO ₂

CaCO ₃→CaO+CO ₂

Also can use from solid state change and (for example, become CO as the sublimator material of gaseous state ₂Dry ice).List other material that when heating produce gas or as NaN3 heating generation nitrogen (2NaN3--->2Na+3N2) in the material list of Fig. 4 B.

Referring to Fig. 5 A, can make breakaway-element between first passage 148 and the second channel 150 and open valve 140 through glass capillary 142 is placed in.Glass capillary 142 has the open end 144 that is positioned in the first passage 148, and is positioned the closing end 146 in the second channel 150.When glass capillary was intact, fluid can't flow between first passage 148 and second channel 150.This is called " closing " state that breakaway-element is opened valve.

Referring to Fig. 5 B, when applying external force with disconnection glass capillary 142, interface channel 148 has formed with the path 152 of passage 150.This is called " opening " state that breakaway-element is opened valve.Breakaway-element is opened valve 140 and can be used for allowing two kinds of fluids (or a kind of fluid and a kind of solid) prima facies to separate, and interacts in the moment by user's control subsequently.

Figure 28 A and Figure 28 B displaying use breakaway-element to open the instance that valve comes the low-cost device of construction, and low-cost device is used for carrying out the calibrating of wherein using ultraviolet (UV) optical radiation fluid.Glass capillary 142 connects two plastic of conduit 460 and 462.Initially, reactant 464 is contained in first plastic of conduit 462.After glass capillary 142 breaks off, reactant 464 glass capillary 142 to second plastic of conduit 460 of flowing through immediately.Shown in Figure 28 B, when reactant 464 is flowed through glass capillary 142, the 466 radioreaction agent 464 of UV light source.Detector 468 detectings are through the UV light of reactant 464.The spectrum of the UV light that detector 468 is detected can be used for judging the compound in the reactant 464.

Figure 28 C shows the xsect of the glass capillary with square inside circumference and neighboring.Square inside circumference and neighboring allow UV light edge to pass through glass capillary perpendicular to the direction on the surface of glass capillary.Having circular cross section with kapillary compares; This mode that kapillary has square cross section allows more UV light to reach the fluid in the glass capillary, and wherein circular cross section possibly cause that incident UV light is along away from the reflection of the direction of fluid or reset to (being redirected).

Referring to Fig. 6 A and Fig. 6 B, can make self-closing valve 160 in the passage 164 through super absorbent polymer (Superabsorbent polymer, beneath abbreviation " SAP ") 162 is placed in.Initially, SAP 162 has smaller size smaller and allows between the first area 166 of fluid in passage 164 and the second area 168 mobile (Fig. 6 A).This is called self-closing valve and " opens " state.When fluid was flowed through SAP 162, the part of SAP fluid-absorbent and volumetric expansion, thereby blocking-up passage 164 (Fig. 6 B) stoped fluid between first area 166 and second area 168, further to flow thus.This is called self-closing valve and " closes " state.

Super absorbent polymer is adsorbable and keep the big volume of water or other WS.In some instance, SAP can be by processing through the starch of chemical modification and cellulose and other polymkeric substance, such as, high-hydrophilic and to glassware for drinking water arranged gathering (vinyl alcohol) PVA, gathering (ethylene oxide) PEO of high-affinity.In some instance, super absorbent polymer can be processed by part neutralization and lightly crosslinked gathering (acrylic acid), and part neutralization and lightly crosslinked gathering (acrylic acid) have the ratio of good usefulness relative cost.Polymkeric substance can manufacture has low solid content, subsequently polymkeric substance drying and grinding is the white particulate solid.In water, white solid is expanded to can comprise the rubber gels of percentage by weight up to 99% water in some cases.

Referring to Fig. 7 A, self-closing valve 170 can comprise passage 164, and passage 164 has enlargement steps down 172 to hold super absorbent polymer 162, makes super absorbent polymer 162 flowing of limit fluid not before expansion.In order to make self-closing valve 170, can binder be coated to the inwall of enlargement steps down 172, be pushed into subsequently in the passage 164 with the SAP 162 of powder morphology, make SAP 162 powder be adhered to the inwall at enlargement steps down 172 places.

Referring to Fig. 7 B, when fluid is flowed through super absorbent polymer 162, the part and the volumetric expansion of super absorbent polymer 162 fluid-absorbent, thereby blocking-up passage 164, the polymkeric substance 162 that stops fluid further to flow through and expand thus.

Referring to Fig. 8 A and Fig. 8 B, super absorbent polymer 162 can be attached to lead 180, is placed in subsequently in the passage 164.Passage 164 can have pocket regions 182, is coated with binder in the pocket regions 182 and defines the position in advance so that lead 180 is anchored on.

Referring to Fig. 8 C, when fluid is flowed through super absorbent polymer 162, the part and the volumetric expansion of polymkeric substance 162 fluid-absorbent, thereby blocking-up passage 164, the polymkeric substance 162 that stops fluid further to flow through and expand thus.

Can through be coated with SAP lead, subsequently will through the coating lead be placed in passage or the pipe in make self-closing valve.Can be through being coated with planar substrates with SAP, will being placed in and making the self-closing valve that is used for the planar flows body device in the plane channel in the planar flows body device through the substrate of coating subsequently.

Referring to Fig. 9 A to Fig. 9 C, can make switch through the SAP 162 that uses glass capillary 142 and be positioned kapillary 142 outsides and adjacent capillaries 142 and drive valve 190.Kapillary 142 all is positioned to have in the passage 164 of first area 166 and second area 168 with SAP162.Use glass capillary 142 and SAP to be similar to the combination of using breakaway-element to open valve and self-closing valve.Switch open valve 190 make the user can through allow, blocking-up subsequently and allow fluid to control fluid the flowing of ad-hoc location in the passage of flowing through subsequently through ad-hoc location.

Referring to Fig. 9 A, initial, SAP 162 has smaller size smaller and does not block passage, thereby allows fluid between first area 166 and second area 168, to flow.

Referring to Fig. 9 B, when fluid through out-of-date, the part of fluid is by SAP 162 absorption, thereby makes the SAP162 volume increase, thus block fluid further mobile between first area 166 and second area 168.

Referring to Fig. 9 C, when applying external force, produce path 152 and between first area 166 and second area 168, flow to allow fluid with disconnection glass capillary 142.

Referring to Figure 10 A to Figure 10 C, can make pass switch valve 200 through using glass capillary 142 and being positioned kapillary 142 inner SAP 162.Kapillary 142 has open end 144 and closing end 146.Open end 144 is positioned in the first passage 148, and closing end 146 is positioned in the second channel 150.Glass capillary 142 and SAP 162 carry out and are similar to the function that breakaway-element is opened the combination of valve and self-closing valve.Close switch valve 200 make the user can be through blocking-up, subsequently allow and subsequently block fluid control fluid the flowing of ad-hoc location in the passage of flowing through through ad-hoc location.

Referring to Figure 10 A, when glass capillary 142 was intact, first passage 148 was not connected with second channel 150.

Referring to Figure 10 B, when applying external force, form path 152, thereby allow fluid between

passage

148 and 150, to flow with disconnection glass capillary 142.SAP 162 initially has smaller size smaller and non-block fluid flowing in path 152.

Referring to Figure 10 C, when fluid was flowed through path 152, the part of fluid was by SAP 162 absorption, thereby made the SAP volume increase and blocking-up path 152, stoped the fluid path 152 of further flowing through thus.

Referring to Figure 11 A to Figure 11 D, can be through using glass capillary 142, be positioned the inner SAP 212 of kapillary 142, and be positioned kapillary 142 outside SAP 214 and make switch switch valve (on-off-on-off valve).Glass capillary 142, SAP 212, and SAP 214 is placed in the passage 164.Glass capillary 142, SAP 212, and SAP 214 execution are similar to the function that breakaway-element is opened the combination of valve and two self-closing valves.Switch switch valve 210 make the user can through allow, blocking-up subsequently, subsequently allow and subsequently block fluid control fluid the flowing of ad-hoc location in the passage of flowing through through ad-hoc location.

Referring to Figure 11 A, initial, SAP 214 has smaller size smaller and allows fluid in mobile between the first area 166 of passage 164 and the second area 168.

Referring to Figure 11 B, when fluid through out-of-date, the part of fluid is by SAP 214 absorption, thereby makes SAP 214 volumes increase, thus block fluid further mobile between first area 166 and second area 168.

Referring to Figure 11 C, when applying external force, form path 152 and between first area 166 and second area 168, flow to allow fluid with disconnection glass capillary 142.

Referring to Figure 11 D, when fluid was flowed through SAP 212, the part of fluid was by SAP 212 absorption, thereby made SAP 212 volumes increase and blocking-up path 152, stoped the fluid path 152 of further flowing through thus.

Referring to Figure 12, can come construction to be used to draw the measuring pipet(te) (Metering Pipette) 220 of the fluid of scheduled volume through the vacuum pump 222 that use is coupled to suction pipe body 224.Vacuum pump 222 comprises the vacuum glass kapillary 100 that is placed in the suction pipe spheroid 226.In order to use measuring pipet(te) 220, break off glass capillary 100 and fluid is drawn the attractive force to the suction pipe body 224 to produce.

When making a collection of measuring pipet(te) 220, the size of spheroid 226 and glass capillary 100 can be made into identical.Spheroid 226 and glass capillary 100 to push spheroid 226 as the user when breaking off glass capillary 100, causes that it is that cardinal principle is identical that glass capillary 100 breaks off the needed deflection of bestowing spheroid 226 through design for all measuring pipet(te)s 220 for.In this way, the user can use measuring pipet(te) 220 under need not to monitor the situation of the fluid content in the suction pipe body 224, to draw the fluid of scheduled volume fast.

For example, referring to Figure 21 A and Figure 21 B, can use measuring pipet(te) 220 fast from the take a sample blood 370 of scheduled volume of patient.

Referring to Figure 13, another instance of measuring pipet(te) 230 comprises vacuum pump 222 and gas pump 232.Vacuum pump 222 is similar to vacuum pump shown in Figure 12.Gas pump 232 comprises glass capillary 120, and glass capillary 120 is filled with Na ₂CO ₃And be placed in and contain CH ₂In the suction pipe spheroid 234 of COOH.When glass capillary 120 breaks off, Na ₂CO ₃With CH ₂COOH interacts to produce CO ₂Thereby, increase the gaseous tension in the spheroid 234.Vacuum pump 222 allows the user to draw fast in fluid to the suction pipe 230 of scheduled volume.Gas pump 232 permission users execute fluid and are assigned to outside the suction pipe 230.

The advantage of using gases pump 232 is: work as Na ₂CO ₃With CH ₂Reaction between the COOH produces CO ₂During gas, can during controlled a period of time, execute the fluid in the pipe arrangement body 228.In this way, the user need not the careful outflow of monitoring fluid when executing dispense fluid.

Referring to Figure 14 A, another instance of measuring pipet(te) 240 comprises spheroid 242, center section 244 and suction pipe body 246.Center section 244 is by the deformable material construction.Switch leaves valve 248 and is positioned in the center section 244.The SAP 162 that valve 248 comprises glass capillary 142 and is positioned kapillary 142 outsides left by switch, and this is similar to the device shown in Fig. 9 A to Fig. 9 C.

Referring to Figure 14 A, in order to use suction pipe 240, user's extruding and release spheroid 242 are with in draw fluid to body 246 and the center section 244.

Referring to Figure 14 B, when fluid reached center section 244 and beginning and contacts with SAP 248, the part of fluid was by SAP 248 absorption, thereby made the fluid passage of opposite side of SAP 248 volumetric expansions and blocking-up SAP 248.In this way, the fluid of scheduled volume is drawn to suction pipe 240.

Referring to Figure 14 C, for self-straw 240 is discharged fluid, the user pushes center section 244 (being processed by deformable material) with disconnection glass capillary 142, thereby forms the path through the kapillary 142 that breaks off.The user pushes spheroid 242 subsequently and flows out suction pipe 240 to force fluid 252.

When making a collection of suction pipe 240; Body 246 and center section 244 are of a size of identical; And the position that the switches in the center section 244 are driven valve 248 is identical, makes the user can use under the situation of the content liquid of suction pipe 240 in need not accurate monitoring suction pipe 240 and draws the fluid of same amount substantially fast.

Referring to Figure 15 A, the WT-MSR 260 that is used to collect the fluid of scheduled volume comprises glass capillary 262, and glass capillary 262 has two 266a of branch and 266b, two self-closing valve 268a and 268b, and two breakaway-elements are opened valve 270a and 270b.Among self-closing valve 268a and the 268b each has the SAP that expands immediately after the fluid-absorbent.Initially, self-closing valve 268a and 268b are in open mode, and breakaway-element is opened valve 270a and 270b is in closed condition.Self-closing valve 268a and 268b can be similar to the self-closing valve shown in Fig. 6 A to Fig. 8 C.Breakaway-element is opened valve 270a and 270b and can be similar to the breakaway-element shown in Fig. 5 A and Fig. 5 B and open valve.

In operation, owing to capillary force, fluid 274 is drawn to kapillary 262, and flow through self-closing valve 268a and 268b.Referring to Figure 15 B; When fluid 274 is flowed through self-closing valve 268a and 268b; The part of fluid 274 is adsorbed by the SAP among self-closing valve 268a and the 268b, thereby makes self-closing valve 268a and 268b be changed to closed condition, and block fluid 274 further flows thus.Cause fluid 274 to occupy the section 264 between self-closing valve 268a capillaceous and the 268b in this way.

Change into open mode and apply attractive force or expulsive force moves fluid 274 through breakaway-element being opened valve 270a and 270b self closing state, fluid 274 can pass through the 266a of branch or 266b moves to other position from section 264.

The advantage of WT-MSR 260 is: WT-MSR 260 can be at the fluid that need not quick sampling predetermined under the careful situation about monitoring of user.Because kapillary has minor diameter, so WT-MSR 260 can be used for the precise sampling small volume of fluid.

Referring to Figure 16 A, can comprise three

kapillary

284a, 284b and 284c from the WT-MSR 280 that sample pool 282 obtains the fluids of three kinds of different amounts.Each kapillary at one end has self-closing valve (for example, 286a, 286b or 286c) and the other end has vacuum valve (for example, 288a, 288b or 288c).Each vacuum pump has the vacuum glass kapillary.Initially, self-closing valve is in open mode.

Referring to Figure 16 B, when the user breaks off the vacuum glass kapillary among the vacuum pump 288a, produce attractive force and draw predetermined amount of liquid to kapillary 284a.When fluid process self-closing valve 286a, the SAP among the self-closing valve 286a expands, thereby makes self-closing valve 286a get into closed condition, stops fluid to be moved further by this and process self-closing valve 286a.Similar, through breaking off the vacuum kapillary among vacuum pump 288b and the 288c, the fluid of scheduled volume can be drawn to kapillary 284b and 284c.The Fluid Volume of being drawn to the kapillary 284a to 284c judged by the volume capillaceous among the vacuum pump 288a to 288c, and the Fluid Volume of being drawn to the kapillary 284a to 284c can be identical or different.

Referring to Figure 17 A, device 290, the two steps calibrating that can be used for the calibrating of two steps through the combination manufacturing of using vacuum pump, breakaway-element to open valve, self-closing valve requires cleaning with damping fluid behind the binding reagents fast.One end of passage 302 is coupled to the sample pool that contains sample 300 through self-closing valve 296, and the other end of passage 302 is coupled to the first vacuum pump 292a.Passage 302 is connected to passage 308, and passage 308 is opened valve 294 through breakaway-element and is coupled to the reservoir that contains damping fluid 298.Passage 302 also is connected to passage 304, and passage 304 is coupled to the second vacuum pump 292b and the 3rd vacuum pump 292c.Passage 304 comprises and combining and/or sensing area 306, in conjunction with and/or sensing area 306 comprise and be used for combining or the reagent of the compound of sensing sample 300.

, combine and sensing area to clean towards combination and sensing area 306 absorption damping fluids 298 subsequently so that draw samples 300 so that reaction takes place with a mode operating means 290 towards combination and sensing area 306.

Referring to Figure 17 B, start vacuum pump 292a and draw to the attractive force of the part of the passage 302 between vacuum pump 292a and the self-closing valve 296 to produce towards vacuum pump 292a absorption sample 300 and with sample 300.When sample 300 was flowed through self-closing valve 296, the part of sample was adsorbed by the SAP in the self-closing valve 296, thereby made self-closing valve 296 get into closed conditions.

Referring to Figure 17 C, start breakaway-element and open valve 294 so that valve 294 is changed into open mode.Start vacuum pump 292b to produce the attractive force of drawing sample 300 and damping fluid 298 towards vacuum pump 292b.So that after starting pump, sample 300 will stop at and combine and sensing area 306 places through design for vacuum pump 292a and 292b.Over time, start vacuum pump 292c moving sample 300 to district 306, and make damping fluid 298 flow through district 306 and cleaning area 306.

Above-mentioned instance provides incubation time (incubation time) to react with the reagent of distinguishing in 306 to allow the compound in the preceding sample 300 of damping fluid 290 cleaning combinations and sensing area 306.If the reaction at 306 places, district is unnecessary for fast and incubation time, then vacuum pump 292b can make big and can omit vacuum pump 292c.When starting vacuum pump 292b, sample is flowed through rapidly and is combined and sensing area 306, is cleaned by damping fluid 298 subsequently to combine and sensing area 306.

Referring to Figure 18 A, device 310, the two steps calibrating that can be used for the calibrating of two steps through the combination manufacturing of using vacuum pump, breakaway-element to open valve, self-closing valve and gas pump requires slowly cleaning with damping fluid behind the binding reagents.The device 310 that is similar to device 290 has passage 302, and passage 302 is connected to two passages 304 and 308.Passage 302 is coupled to sample 300 through self-closing valve 296.Passage 308 is opened valve 294 through breakaway-element and is coupled to damping fluid 298.Passage 304 comprises and combining and sensing area 306.One end of passage 304 is coupled to breakaway-element and opens valve 312.Gas pump 314 is coupled to damping fluid 298.

Device 310 is with the difference of device between 290: device 310 is not to use vacuum pump 292b to come towards combining and sensing area 306 is drawn sample 300 and damping fluids 298, but using gases pump 314 is towards distinguishing 306 promotion sample 300 and damping fluids 298.

Referring to Figure 18 B,, start vacuum pump 292a to draw sample 300 to passage in order to carry out the calibrating of two steps.Get into closed condition at sample flow valve 296 behind self-closing valve 296.

Referring to Figure 18 C, start breakaway-element and

open valve

294 and 312 so that valve is changed into open mode.Start gas pump 314 during a period of time, producing gas, thereby promote sample 300 and damping fluid 298 through combining and sensing areas 306.Because gas pump 314 is producing gas (reaction between the compound of generation gas expends a certain amount of time and accomplishes) during a period of time, sample 300 can slowly pass through and combine and sensing area 306, thereby association reaction slowly takes place.

Referring to Figure 19 A, device 320, the three steps calibrating that can be used for the calibrating of three steps through reservoir and the structure manufacturing shown in passage 322 to Figure 17 A that interpolation contains second damping fluid 324 requires cleaning with two kinds of damping fluids behind the binding reagents fast.Suddenly examine and determine in order to carry out multistep, start vacuum pump 292a so that sample 300 flows to passage 302.When sample 300 was flowed through self-closing valve 296, valve 296 was changed into closed condition.

Referring to Figure 19 B, start breakaway-element and open valve 294, and start vacuum pump 292b so that towards combining and the sensing area 306 absorption sample 300 and first damping fluids 298 so that it changes into open mode.

Referring to Figure 19 C, start breakaway-element and open valve 326, and start vacuum pump 292c so that towards combining and sensing area 306 absorption sample 300, first damping fluid 298 and second damping fluids 324 so that it changes into open mode.In this way, the reaction at available two kinds of 306 places, different buffer solution for cleaning district.

Can come construction to be used for the device of the calibrating of the step more than requirement three steps through the end that couples extra damping fluid or sample and an interpolation corresponding number vacuum pump to passage 304.

Referring to Figure 20, but construction module 330 is carried out the multiple analysis assay.Module comprises sample pool 282 and three chamber 332a, 332b and the 332c that are used for fixing sample 300, and each chamber contains the analyte that is useful on the compound in combination and the sensing sample 300.Below describe in order to carry out with chamber 332a in the assembly of the relevant calibrating of first analyte.

Chamber 332a is coupled to sample pool 282 through passage 342a and self-closing valve 344a.Passage 342a process self-closing valve 346a and breakaway-element are opened valve 348a and are coupled to the first damping fluid 350a.Passage 342a process self-closing valve 352a and breakaway-element are opened valve 354a and are coupled to the second damping fluid 356a.Passage 342a process self-closing valve 358a and breakaway-element are opened valve 360a and are coupled to the 3rd damping fluid 362a.Chamber 332a also is connected to vacuum pump 334a, 336a, 338a and 340a.

In order to carry out calibrating, start vacuum pump 334a to draw sample 300, so that allow compound and analyte 332a in the sample 300 to react towards chamber 332a.After self-closing valve 344a, valve 344a changes into closed condition in a certain amount of sample flow.Open the valve 348a (valve is changed into open mode) and the second vacuum pump 336a through starting breakaway-element, the first damping fluid 350a washed chamber 332a.After a certain amount of first damping fluid 350a flowed through self-closing valve 346a, valve 346a changed into closed condition.

Open valve 354a (valve is changed into open mode) and the 3rd vacuum pump 338a through starting breakaway-element, the second damping fluid 356a washed chamber 332a.After a certain amount of second damping fluid 356a flowed through self-closing valve 352a, valve 352a changed into closed condition.

With this similar mode, open valve 360a (valve is changed into open mode) and the 4th vacuum pump 340a through starting breakaway-element, the 3rd damping fluid 362a washed chamber 332a.After a certain amount of the 3rd damping fluid 362a flowed through self-closing valve 358a, valve 358a changed into closed condition.

Can be similar to carry out with chamber 332a in the mode of the relevant calibrating of first analyte carry out with

chamber

332b and 332c in the calibrating of being correlated with of the second and the 3rd analyte.Can carry out simultaneously with

chamber

332a, 332b and 332c in first, second and the relevant calibrating of the 3rd analyte.

Below be the vacuum pump of the biological calibrating of execution and the application of gas pump.

Figure 22 A and Figure 22 B show the device 380 that is used to carry out the calibrating of rapid reaction colorimetric.Device 380 comprises passage 384, and an end of passage 384 is coupled to sample pool 382 and the other end is coupled to vacuum pump 90.But the fluid of sample pool 382 fixings such as blood or urine.Passage 384 comprises test section 386, and test section 386 has and detects the p-wire that changes color behind a certain compound immediately.When starting, can draw by vacuum pump 90 fluid in the sample pool 382 fast through test section 386.Through the color of read test line, the user can judge fast in the fluid and to have or not exist a certain compound.

Figure 23 A and Figure 23 B show the device 390 that is used to take a sample through the fluid that filters.Device 390 comprises passage 384, and an end of passage 384 is coupled to sample pool 382 and the other end is coupled to vacuum pump 90.Filtration membrane 392 is placed in the sample pool 382.Can draw fluid (for example, blood) in the sample pool 382 when vacuum pump 90 starts fast through filtration membrane 392, thereby produce the fluid of being drawn to the passage 384 (blood plasma) through filtering.

Figure 24 A to Figure 24 C shows the device 400 that is used for colorimetric calibrating slowly.Device 400 comprises sample pool 402, and sample pool 402 is coupled between gas pump 404 and the passage 384.Passage 384 has test section 386, and test section 386 has and detects the p-wire that changes color behind a certain compound immediately.For operative installations 400, sample fluid 406 is placed in the sample pool 402.The opening of band 408 sealing sample pools.Start gas pump 404 and promote the gas of sample fluid 406 through test section 386 to produce.Because gas pump 404 produces gas during a period of time, thus sample fluid 406 during a period of time, advance through the test section, thereby allow to carry out slowly the colorimetric calibrating.

A specific embodiment of the present invention then please with reference to Figure 29 A and 29B, can be used for blood sample is carried out the device 500 of antibody (Antibody) calibrating through the combination manufacturing of using self-closing valve (SLV) and breakaway-element to open valve (BOV).This device 500 comprises that blood sample pond (Blood sample well) 501, dcq buffer liquid pool (Washing buffer well) 503, metering region and sign antibody (Metering zone and labeled antibody) zone 505, diagnosis zone (Diganostic Zone) (antibody array Antibody array) 507, breakaway-element open valve (BOV) 509, discarded object pond (Waste well) 511 and self-closing valve (SLV) 513.This metering region has a channel connection blood sample pond 501 and dcq buffer liquid pool 503 with sign antibody regions 505, and self-closing valve (SLV) 513 then is between this passage.Diagnosis zone 507 then is to have another passage, and an end is connected to metering region and the center that indicates antibody regions 505, and the other end is then opened valve (BOV) 509 through breakaway-element and is connected to discarded object pond (Waste well) 511.

The method that 500 pairs of blood samples of this device carry out antibody (Antibody) calibrating is please with reference to shown in Figure 30 A, 30B and the 30C.At first; Blood sample 502 is loaded in the blood sample pond 501; Then; Use the self-closing valve (SLV) 513 that is proposed in the previous embodiment of the present invention, utilize capillary force (Capillary force) blood to be sucked in the passage of this metering region and sign antibody regions 505 502a shown in Figure 30 A.Then, be written into dcq buffer liquid in dcq buffer liquid pool 503, and this moment, dcq buffer liquid can flow into also in the passage of metering region and sign antibody regions 505, like the 504a of Figure 30 B, and this moment, blood can be squeezed in the diagnosis zone 507, like the 502b of figure.

Then; Please refer to Figure 30 C; After breakaway-element opened valve (BOV) 509 and break off, then blood is further sucked and through diagnosis zone 507,502c as shown in the figure; This moment, the antibody array (Antibody array) in this diagnosis zone 507 will combine with the antigen (Antigent) in the blood, and dcq buffer liquid then will be flushed to discarded object pond 511 less than the blood of effect.

Another specific embodiment of the present invention, and referring to shown in Figure 31.Can be used for blood sample is carried out the device of antibody (Antibody) calibrating through the combination manufacturing of using self-closing valve (SLV) and breakaway-element to open valve (BOV).And this device comprises that blood sample pond, dcq buffer liquid pool, diagnosis zone (intension antibody array), breakaway-element open valve (BOV), discarded object pond and self-closing valve.This blood sample pond then comprises and has the film that removes haemocyte (Blood cell removal), and blood sample capable of using can filter out haemocyte when sucking.

This diagnosis zone has a channel connection blood sample pond and dcq buffer liquid pool, and the other end is then opened valve (BOV) through breakaway-element and is connected to the discarded object pond.Self-closing valve (SLV) then is to be positioned at the blood sample pond to get into before this passage, and may command flows into the blood flow volume in diagnosis zone, and prevents that backflow of blood is to the blood sample pond.

After breakaway-element is opened valve (BOV) disconnection; Behind the blood elder generation process membrane filtration of removable haemocyte; Further suck in the diagnosis zone; This moment, the antibody array (Antibody array) in the diagnosis zone will combine with the antigen (Antigent) in the blood, and dcq buffer liquid then will be flushed in the discarded object pond less than the blood of effect.

Although preceding text have been discussed some instance, other enforcement and the category of using the claim that also belongs to appended.For example, in the vacuum pump 90 of Figure 1A and Figure 1B, container 100 can contain lower pressure region and be not to contain vacuum area.As long as container 100 gas inside pressure are lower than the gaseous tension of container 100 outsides, when container 100 breaks off, the pressure in the zone 106 of container 100 outsides will descend, thereby produce along the attractive force of a direction towards container 100 draw fluid.Above-described kapillary can be substituted by the kapillary of processing with other hard brittle material, such as brittle plastic, quartz and ceramic.

Claims

1. fluid control method comprises:

Control fluid flowing in passage; Comprise and break off first container in said passage, to produce pressure differential; Make said fluid in said passage, move; Said first container is processed by hard brittle material and after applying external force, is broken off immediately, and wherein said first container defines the space in said first container, and said space has the gaseous tension of the gaseous tension that is different from said first external container.

2. fluid control method comprises:

Control fluid flowing in passage; Comprise and break off first container in said passage, to produce pressure differential; Make said fluid in said passage, move; Said first container is processed by hard brittle material and after applying external force, is broken off immediately, and wherein said first container comprises first material, and said first material is separated with second material that is positioned at said first external container before said first container breaks off; When applying external force when breaking off said first container, said first material and said second material through selecting so that after said first material and the interaction of said second material, produce gas immediately.

3. according to claim 1 or claim 2 fluid control method, the space in wherein said first container has the pressure that is higher than the said first external container pressure.

4. like claim 2 or 3 described fluid control methods, wherein control said fluid said flow to be contained in promote said fluid in the said passage and make it away from said first container that is disconnected.

5. fluid control method as claimed in claim 1, the space in wherein said first container has the pressure that is lower than the said first external container pressure.

6. fluid control method as claimed in claim 5, the said said fluid that comprises in the said passage of attraction that flows of wherein controlling said fluid makes it towards said first container that is disconnected.

7. fluid control method as claimed in claim 6, wherein said fluid comprises blood, and control said blood said flow comprise make said blood through filtrator with the occlude blood cell and allow blood plasma through said filtrator and get into said passage.

8. fluid control method as claimed in claim 6 more comprises and when said fluid flows, carry out the colorimetric calibrating in said passage.

9. fluid control method as claimed in claim 6 more comprises second container, and said second container defines the space in said second container, and said space has the gaseous tension of the gaseous tension that is higher than said second external container.

10. fluid control method as claimed in claim 6; More comprise second container; Said second container comprises the 3rd material; Said the 3rd material is separated with the 4th material before said second container breaks off, said the 3rd material and said the 4th material through selecting so that produce gas immediately after said the 3rd material and the interaction of said the 4th material.

11. like claim 9 or 10 described fluid control methods, the said said fluid that comprises in the said passage of promotion that flows of wherein controlling said fluid makes it away from said second container.

12. fluid control method as claimed in claim 6, said flow of wherein controlling said fluid comprises the fluid adsorption material that expands immediately through volume after the part of using the said fluid of absorption and stops additional fluid to move along a certain direction to pass through said passage.

13. a fluid means comprises:

Passage; And

First container; Said first container produces pressure differential in said passage when said first container breaks off; Said first container is processed by hard brittle material and after applying external force, is broken off immediately; Wherein said first container defines the space in said first container, and said space has the gaseous tension of the gaseous tension that is different from said first external container.

14. a fluid means comprises:

Passage; And

First container; Said first container produces pressure differential in said passage when said first container breaks off; Said first container is processed by hard brittle material and after applying external force, is broken off immediately; Wherein said first container comprises first material; Said first material is separated with second material that is positioned at said first external container before said first container breaks off, when applying external force when breaking off said first container, said first material and said second material through selecting so that produce gas immediately after said first material and the interaction of said second material.

15. like claim 13 or 14 described fluid means, more comprise second container, said second container defines the space in said second container, this space of said second container has the gaseous tension of the gaseous tension that is different from said second external container.

16. like claim 13 or 14 described fluid means; More comprise second container; Said second container comprises the 3rd material; Said the 3rd material is separated with the 4th material before said second container breaks off, said the 3rd material and said the 4th material through selecting so that produce gas immediately after said the 3rd material and the interaction of said the 4th material.

17. fluid means as claimed in claim 15, the space in wherein said first container has the pressure that is higher than the said first external container pressure, and the space in said second container has the pressure that is lower than the said second external container pressure.

18. fluid means as claimed in claim 13, the said space in wherein said first container has the pressure that is lower than the said first external container pressure;

This fluid means more comprises second container; Said second container defines the space in said second container; This space of said second container has the gaseous tension of the gaseous tension that is different from said second external container; Said second container comprises first material, and said first material is separated with second material before said second container breaks off, said first material and said second material through selecting so that produce gas immediately after said first material and the interaction of said second material.

19. fluid means as claimed in claim 18 more comprises filtration membrane with occlude blood cell and permission blood plasma process.

20. like claim 13 or 14 described fluid means, wherein said hard brittle material comprises at least one in the following material: quartz, glass, pottery or plastics.

21. like claim 13 or 14 described fluid means, wherein said passage is to be defined by the wall of processing with the pliability material.

22. produce the material of gas when fluid means as claimed in claim 13, wherein said first container comprise heating.

23. like claim 13 or 14 described fluid means, wherein said first container comprises when heating from the solid-state material that sublimes up into gaseous state.

24. a fluid means comprises:

The sample pool that contains first fluid;

First reservoir that contains second fluid;

The main channel;

First branched bottom, said first branched bottom couple an end of said sample pool to said main channel;

Second branched bottom, said second branched bottom couple said first reservoir to said main channel;

First single uses pump, is communicated in the other end of this main channel, and said first single uses pump to produce pressure differential to move one in said first fluid and said second fluid or both when said first single uses container in the pump to break off;

Second single uses pump; Be communicated between the two ends of this main channel; Said second single uses pump to produce pressure differential to move one in said first fluid and said second fluid or both when said second single uses container in the pump to break off; Wherein said first single uses pump (a) to define said first single and uses the space in the pump; Said space has the gaseous tension that is different from the outside gaseous tension of said first single use pump; Or (b) comprise first material, said first material uses the second outside material of pump to be separated with being positioned at said first single before said first single uses pump to break off, said first material and said second material through selecting so that produce gas immediately after said first material and the interaction of said second material; Said second single uses pump (a) to define said second single and uses the space in the pump; Said space has the gaseous tension that is different from the outside gaseous tension of said second single use pump; Or (b) comprise first material; Said first material uses the second outside material of pump to be separated with being positioned at said second single before said second single uses pump to break off, said first material and said second material through selecting so that produce gas immediately after said first material and the interaction of said second material.

25. fluid means as claimed in claim 24; More comprise self-closing valve; Said self-closing valve comprises initially having small size so that said first fluid can pass through the material of said valve, and said material increases volume and further passes through the path of said valve to stop said fluid after the part of the said first fluid of absorption.

26. fluid means as claimed in claim 24; More comprise valve; Said valve has the connector of processing with hard brittle material, and wherein when said connector was intact, said valve stoped said first fluid to get into said main channel; And when said connector breaks off, produce path and get into said main channel to allow said first fluid.

27. fluid means as claimed in claim 24 more comprises: second reservoir, said second reservoir contains three-fluid; And the 3rd branched bottom, said the 3rd branched bottom couples this second reservoir to said main channel.

28. fluid means as claimed in claim 24 more comprises sensing area, said sensing area is coupled to said main channel, and said sensing area comprises whether the decidable certain material is present in the reagent in the said first fluid.

29. a fluid control method comprises:

A plurality of suction pipes are provided so that the fluid of the scheduled volume of can taking a sample, each suction pipe comprises:

Passage, and

Container; Said container produces pressure differential when breaking off in said passage; Said container is processed by hard brittle material and after applying external force, is broken off immediately; Said container defines the space in the said container, and said space has the gaseous tension less than the gaseous tension of said external container, its break said container can in said passage, produce scheduled volume pressure differential so that the fluid of scheduled volume drawn to said passage.

30. a fluid control method comprises:

First container that disconnection is processed by hard brittle material is to produce pressure differential in passage; Make first fluid move to first section of said passage from first reservoir; Said first container (a) defines the space in said first container, and said space has the gaseous tension of the gaseous tension that is different from said first external container; And

Second container that disconnection is processed by hard brittle material to be to produce pressure differential in passage, make the part at least of said first fluid move and through second section of said passage.

31. a fluid control method comprises:

First container that disconnection is processed by hard brittle material is to produce pressure differential in passage; Make first fluid move to first section of said passage from first reservoir; Said first container comprises first material; Said first material is separated with second material that is positioned at said first external container before said first container breaks off, said first material and said second material through selecting so that produce gas immediately after said first material and the interaction of said second material.

32. like claim 30 or 31 described fluid control methods, more comprise first valve that disconnection processed by hard brittle material to produce first path, said first path connects second reservoir to said passage, said second reservoir contains second fluid.

33., wherein make said second fluid move to said second section of said passage from said second reservoir through breaking off the said pressure differential that said second container produces like claim 31 or 32 described fluid control methods.

34. like claim 31 or 33 described fluid control methods, more comprise second valve that disconnection processed by hard brittle material to produce alternate path, said alternate path connects the 3rd reservoir to said passage, said the 3rd reservoir contains three-fluid.

35. like claim 31 or 34 described fluid control methods, more comprise the 3rd container that disconnection processed by hard brittle material, make said three-fluid move to said second section of said passage from said the 3rd reservoir to produce pressure differential.

36. like claim 30 or 31 described fluid control methods, said second section of wherein said passage comprises in order to judge whether specific judgement material is present in the reagent in the said first fluid.

37. fluid control method as claimed in claim 30, wherein, said first container defines the space in said first container, and said space has the gaseous tension of the gaseous tension that is lower than said first external container.

38. fluid control method as claimed in claim 37, wherein said second container define the space in said second container, this space of said second container has the gaseous tension of the gaseous tension that is lower than said second external container.

39. fluid control method as claimed in claim 37; Wherein said second container defines the space in said second container; This space of said second container has gaseous tension, and this gaseous tension in this space of said second container is higher than the gaseous tension of said second external container.

40. a fluid control method comprises:

Operate simultaneously first single use the pump and second single use pump with first to the first passage of drawing sample fluid and the second portion of drawing said sample fluid to second channel; Comprise that first container that breaks off in said first single use pump is to produce pressure differential; Make the said first of said sample fluid move to said first passage from sample pool; And break off said second single and use second container in the pump, make the said second portion of said sample fluid move to said second channel from said sample pool to produce pressure differential; And

Operating the 3rd single simultaneously uses pump and the 4th single to use pump to draw first buffer solution to said first passage and to draw second buffer solution to said second channel; Comprise that the 3rd container that breaks off in said the 3rd single use pump is to produce pressure differential; Make this first buffer solution move to said first passage from first reservoir; And break off said the 4th single and use the 4th container in the pump, make second buffer solution move to said second channel from second reservoir to produce pressure differential;

Wherein said first single uses pump to define said first single and uses the space in the pump, and said space has the gaseous tension that is different from the outside gaseous tension of said first single use pump; Said second single uses pump to define said second single and uses the space in the pump, and said space has the gaseous tension that is different from the outside gaseous tension of said second single use pump; Said the 3rd single uses pump to define said the 3rd single and uses the space in the pump, and said space has the gaseous tension that is different from the outside gaseous tension of said the 3rd single use pump; Said the 4th single uses pump to define said the 4th single and uses the space in the pump, and said space has the gaseous tension that is different from the outside gaseous tension of said the 4th single use pump;

41. fluid control method as claimed in claim 40; More comprise and operate the 5th single simultaneously and use pump and the 6th single to use pump to draw tri buffer solution to said first passage and to draw the 4th buffer solution to said second channel; Comprise that the 5th container that breaks off in said the 5th single use pump is to produce pressure differential; Make this tri buffer solution branch move to said first passage from the 3rd reservoir; And break off said the 6th single and use the 6th container in the pump, make the 4th buffer solution move to said second channel from the 4th reservoir to produce pressure differential;

Said the 5th single uses pump to define said the 5th single and uses the space in the pump, and said space has the gaseous tension that is different from the outside gaseous tension of said the 6th single use pump; Said the 6th single uses pump to define said the 6th single and uses the space in the pump, and said space has the gaseous tension that is different from the outside gaseous tension of said the 6th single use pump.

42. fluid control method as claimed in claim 40; More comprise with said first single use pump operate simultaneously the 5th single use pump with the third part of drawing said sample fluid to third channel; And using pump to operate the 6th single simultaneously with said the 3rd single uses pump to draw tri buffer solution to said third channel; Comprise that the 5th container that breaks off in said the 5th single use pump is to produce pressure differential; Make the third part of this sample fluid move to said third channel, and break off said the 6th single and use the 6th container in the pump, make tri buffer solution move to said third channel from the 3rd reservoir to produce pressure differential from this sample pool;

Wherein said the 5th single uses pump to define said the 5th single and uses the space in the pump, and said space has the gaseous tension that is different from the outside gaseous tension of said the 6th single use pump; Said the 6th single uses pump to define said the 6th single and uses the space in the pump, and said space has the gaseous tension that is different from the outside gaseous tension of said the 6th single use pump.