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Publication numberCN101004424 B
Publication typeGrant
Application numberCN 200610033283
Publication date27 Apr 2011
Filing date20 Jan 2006
Priority date20 Jan 2006
Also published asCN101004424A
Publication number200610033283.5, CN 101004424 B, CN 101004424B, CN 200610033283, CN-B-101004424, CN101004424 B, CN101004424B, CN200610033283, CN200610033283.5
Inventors侯金龙, 朱星才, 李伟
Applicant深圳迈瑞生物医疗电子股份有限公司
Export CitationBiBTeX, EndNote, RefMan
External Links: SIPO, Espacenet
Device and method for detecting jam of sample needle of biochemical analyser
CN 101004424 B
Abstract
A device used for detecting out blocked specimen needle of biochemical analyzer is prepared for setting specimen needle to by variable impedance and variable capacity type, arranging liquid level detection circuit on detection circuit, switching specimen needle as variable impedance into liquid level detection circuit, detecting impedance variation of said variable impedance by liquid level detection circuit and outputting detected signal. The method utilizing said device to detect blocked specimen needle on biochemical analyzer is also disclosed.
Claims(12)  translated from Chinese
1. 一种生化分析仪样本针堵塞检测装置,包括样本针G)、检测电路(1)、控制板0), 所述检测电路具有CPU(S)、接口电路(9),其特征是:所述样本针(4)为可变阻抗;所述检测电路(1)包括液面检测电路(5);所述样本针(4)作为可变阻抗接入到液面检测电路(5) 中,液面检测电路(5)检测所述可变阻抗的阻抗变化Vb-Va,并将检测信号输出至CPU(8), 当所述可变阻抗的阻抗变化Vb-Va大于某一个阈值设为M, CPU(S)就通过接口电路(9) 输出堵塞信号给控制板O),表示样本针(4)被堵塞了;其中Va为初始状态时液面检测电路工作电压,Vb为生化分析仪运行后的液面检测电路工作电压。 A biochemical analyzer sample needle clogging detecting means includes a sample needle G), detection circuit (1), the control panel 0), the detection circuit having a CPU (S), an interface circuit (9), characterized in that: The sample needle (4) is variable impedance; the detection circuit (1) comprises a liquid level detection circuit (5); the sample needle (4) access to the variable impedance as the liquid level detection circuit (5) , the impedance of variable impedance variation detecting the liquid level detection circuit (5) Vb-Va, and outputs a detection signal to the CPU (8), when the impedance of the variable impedance change Vb-Va is larger than a threshold value is set to a M, CPU (S) to output a signal to the control panel jam O), shows a sample needle (4) is blocked by the interface circuit (9); where Va is the initial state of the liquid level detection circuit voltage, Vb biochemical analyzer After running the liquid level detection circuit voltage.
2.如权利要求1所述的生化分析仪样本针堵塞检测装置,其特征是:所述可变阻抗为可变电容。 Biochemical analyzer sample needle according to claim 2. The clogging detecting means, characterized in that: said variable impedance is a variable capacitance.
3.如权利要求2所述的生化分析仪样本针堵塞检测装置,其特征是:所述可变电容为双层同轴结构,内层(4a)与外层(4b)之间用绝缘材料(4c)隔绝,内层(4a)和外层(4b) 分别作为可变电容的两个极板。 3. The biochemical analyzer sample needle clogging detecting apparatus according to claim 2, characterized in that: the variable capacitance is double coaxial structure, between the inner layer (4a) and the outer layer (4b) of an insulating material (4c) isolated from the inner layer (4a) and an outer layer (4b), respectively, as the two plates of a variable capacitor.
4.如权利要求1或2或3所述的生化分析仪样本针堵塞检测装置,其特征是:所述液面检测电路(5)包括振荡源(51)、仪表放大器(52)和平衡电路,所述振荡源(51)所产生的信号(Vin) —路经平衡电路的第一支路输入到仪表放大器(52)的负输入端(V-),另一路经平衡电路的第二支路输入到仪表放大器的正输入端(V+),样本针内外两层分别作为可变电容(Cp)的两个极接入到平衡电路的第二支路中;所述平衡电路设定为当可变电容(Cp) 的电容值为特定值时,其输入到仪表放大器(5的正输入端(V+)和负输入端(V-)的信号保持平衡,当可变电容(Cp)的电容值偏离特定值时,其输入到仪表放大器(5的正输入端(V+)和负输入端(V-)的信号产生差异。 The biochemical analyzer sample needle clogging detecting device 1 or 2 or 3 of the preceding claims, characterized in that: the liquid level detection circuit (5) comprises an oscillation source (51), an instrumentation amplifier (52) and balancing circuit , the oscillation signal source (51) generated by the (Vin) - pass the first balanced circuit tributary input to the instrumentation amplifier (52) of the negative input (V-), another pass balanced circuit's second input to the positive input of the instrumentation amplifier (V +), a sample needle outer and inner layers, respectively, as the two variable capacitance (Cp) of pole access to a balanced circuit in a second branch; the balancing circuit is set when variable capacitance (Cp) of the capacitance value of a specific value, which is input to the instrumentation amplifier (positive input terminal (V +) and the negative input terminal (V-) balance 5 signal, when the variable capacitance (Cp) of the capacitor When the value of the deviation from the specified value, which is input to the instrumentation amplifier (positive input terminal (V +) and the negative input terminal (V-) signal 5 of a difference.
5.如权利要求1或2或3所述的生化分析仪样本针堵塞检测装置,其特征是:还包括上位机(3),所述控制板⑵一端与检测电路⑴相连,另一端与上位机(3)相连;所述检测电路(1)中还包括有依次相连的A/D变换电路(7),所述A/D变换电路(7)的输入端与液面检测电路(5)相连。 5. biochemical analyzer sample needle 1 or 2 or claim 3, wherein the blockage detection means, characterized by: further comprising a host computer (3), the control panel ⑴ ⑵ detection circuit is connected at one end to the other end with the host machine (3) is connected; said detection circuit (1) further comprises sequentially connected an A / D converting circuit (7), the A / D converting circuit (7) and the input of the level detecting circuit (5) connected.
6.如权利要求5所述的生化分析仪样本针堵塞检测装置,其特征是:所述检测电路(1) 中还包括有温度传感电路(6),其输出端与A/D变换电路(7)的另一输入端相连;所述温度传感电路(6)检测所述样本针(4)所处环境的温度变化,并将温度信号输出至CPU(S);CPU(S)将因堵塞造成的工作电压变化量(V)按如下公式校正:其中Vt为可变阻抗工作电压与温度的变化率,Tl为初始状态环境温度,Va为初始状态时液面检测电路工作电压,Vb为生化分析仪运行后的液面检测电路工作电压,T2为通过温度传感电路信号得到的Vb时的环境温度; 当V大于阈值(Δν)则判断样本针堵塞。 6. biochemical analyzer sample needle clogging detection apparatus according to claim 5, characterized in that: said detecting circuit (1) further comprises a temperature sensing circuit (6), the output terminal of A / D conversion circuit (7) is connected to the other input terminal; said temperature sensing circuit (6) detects said sample needle (4) in which the temperature changes in the environment, and the temperature signal to the CPU (S); CPU (S) to Working voltage variation caused by clogging (V) corrected by the following formula: where Vt is the operating voltage of the variable impedance rate of change of temperature, Tl ambient temperature to the initial state, Va is the initial state when the voltage level detection circuit, Vb biochemical analyzer run after the liquid level detection circuit voltage, T2 is the ambient temperature by a temperature sensing circuit when the signal Vb obtained; and when V is greater than the threshold value (Δν) it is determined that the sample needle clogging.
7. —种生化分析仪样本针堵塞检测方法,其特征是包括如下步骤: Α、将样本针(4)作为可变阻抗接入到液面检测电路(5)中;B、利用检测电路(5)检测所述可变阻抗的阻抗变化Vb-Va,并将检测信号输出;其中Va 为初始状态时液面检测电路工作电压,Vb为生化分析仪运行后的液面检测电路工作电压;C、根据输出的信号判断样本针是否堵塞:当所述可变阻抗的阻抗变化Vb-Va大于某一个阈值设为Δν,表示样本针被堵塞。 7. - Species biochemical analyzer sample needle clogging detecting method, characterized by comprising the steps of: Α, the sample needle (4) access to the variable impedance as the liquid level detection circuit (5); B, using the detection circuit ( 5) detecting the impedance of the variable impedance change Vb-Va, and outputs a detection signal; wherein Va is the initial state of the liquid level detection circuit voltage, Vb biochemical analyzer run after the liquid level detection circuit voltage; C The signal output is determined whether the sample needle blockage: When the impedance of the variable impedance change Vb-Va is larger than a threshold value is set to a Δν, it shows a sample needle is clogged.
8.如权利要求7所述的生化分析仪样本针堵塞检测方法,其特征是:所述可变阻抗为可变电容。 7 biochemical analyzer sample needle according to claim 8. A method of detecting the clogging, characterized in that: said variable impedance is a variable capacitance.
9.如权利要求7或8所述的生化分析仪样本针堵塞检测方法,其特征是步骤B中检测电路(5)检测所述可变阻抗的阻抗变化的方法包括如下步骤:Bi、利用振荡源(51)所产生信号(Vin);Β2、将所述信号一路经平衡电路的第一支路输入到仪表放大器(52)的负输入端(V-), 另一路经平衡电路的第二支路输入到仪表放大器的正输入端(V+);Β3、仪表放大器将检测到的信号输出;其中,样本针内外两层分别作为可变电容(Cp)的两个极接入到平衡电路的第二支路中;所述平衡电路设定为当可变电容(Cp)的电容值为特定值时,其输入到仪表放大器(52)的正输入端(V+)和负输入端(V-)的信号保持平衡,当可变电容(Cp)的电容值偏离特定值时,其输入到仪表放大器(5的正输入端(V+)和负输入端(V-)的信号产生差异。 Biochemical analyzer sample needle clogging detection method according to claim 7 or claim 8, wherein in step B the detection circuit (5) detecting the impedance of the variable impedance variation method comprising the steps of: Bi, the use of oscillation source (51) generated by the signal (Vin); Β2, the signal input all the way through the first leg of the balanced circuit to an instrumentation amplifier (52) to the negative input terminal (V-), the other passing a second balanced circuit tributary input to the positive input of the instrumentation amplifier (V +); Β3, instrumentation amplifier outputs the detected signal; wherein the sample needle outer and inner layers, respectively, as the two variable capacitance (Cp) of pole access to balanced circuit The second branch; the balancing circuit is set when the variable capacitance (Cp) of the capacitance value of particular value when its input to the instrumentation amplifier (52) of the positive input terminal (V +) and the negative input terminal (V- ) signal to maintain a balance, when the variable capacitance (when Cp) of capacitance values deviate from the specific value, which is input to the instrumentation amplifier (positive input 5 of (V +) and the negative input terminal (V-) of the signal differences.
10.如权利要求7所述的生化分析仪样本针堵塞检测方法,其特征是在步骤B之后还包括如下步骤:将输出的检测信号依次输入到A/D变换电路(7)、CPU(S)、接口电路(9)、控制板O)、 上位机⑶。 10. The sample needle biochemical analyzer according to claim 7 clogging detecting method, characterized by further comprising the step of, after the step B: the detection signal is sequentially inputted to the A / D converting circuit (7), CPU (S ), an interface circuit (9), the control panel O), PC ⑶.
11.如权利要求7或8所述的生化分析仪样本针堵塞检测方法,其特征是:在步骤B中还检测环境温度,并将环境温度检测值依次输入到A/D变换电路(7)、 CPU(S)、接口电路(9)、控制板(2)、上位机(3)。 11. The biochemical analyzer sample needle 7 or claim 8, wherein the clogging detection method, wherein: in step B is also detected ambient temperature, and the ambient temperature detection value sequentially input to the A / D converting circuit (7) , CPU (S), an interface circuit (9), the control panel (2), PC (3).
12.如权利要求11所述的生化分析仪样本针堵塞检测方法,其特征是:将因堵塞造成的工作电压变化量V按如下公式校正: 12. The 11 samples of the biochemical analyzer of claim needle clogging detecting method, characterized in that: the voltage change amount of work caused by clogging of the correction by the following formula V:
Figure CN101004424BC00031
其中Vt为可变阻抗工作电压与温度的变化率,Tl为初始状态环境温度,Va为初始状态时液面检测电路工作电压,Vb为可变阻抗改变后的液面检测电路工作电压,T2为通过温度传感电路信号得到的Vb时的环境温度;当V大于阈值ΔV则判断样本针堵塞。 Where Vt is the rate of change of the variable impedance operating voltage and temperature, Tl ambient temperature to the initial state, Va is the initial state of the liquid level detection circuit voltage, Vb is changed after the variable impedance level detection circuit voltage, T2 is Vb when the ambient temperature by a temperature sensing circuit signal obtained; and when V is greater than the threshold value ΔV is determined that the sample needle clogging.
Description  translated from Chinese

生化分析仪样本针堵塞检测装置及方法【技术领域】[0001] 本发明涉及生化分析仪样本针堵塞检测装置及方法。 Biochemical analyzer sample needle clogging detection device and method TECHNICAL FIELD [0001] The present invention relates to a biochemical analyzer sample needle clogging detection apparatus and method. 【背景技术】[0002] 对于全自动生化分析仪来说,液面检测针特别是样本针,很可能因为吸入纤维蛋白或血细胞而堵塞,造成加样不准确,甚至无法正常加样,在操作者不知情的情况下这将严重影响测量结果,所以很有必要对样本针的堵塞情况进行判断。 BACKGROUND ART [0002] For automatic biochemical analyzer, the liquid level detection sample needle needle especially, probably because inhalation fibrin or blood cells clog, resulting sample is not accurate, not even normal sample, in operation the case without the knowledge of which would seriously affect the measurement results, so it is necessary to sample needle clogging judge. [0003] 为此,在专利US6,663,353中描述了一种样本针堵塞检测系统,包括两个检测传感部分,一部分实现液面检测,另一部分通过压力传感器来检测液路通道内压力的变化从而判断液面检测针的状况。 [0003] For this reason, in Patent US6,663,353 describes a sample needle clogging detection system comprising two detector sensing portion, a portion of the liquid level detection to achieve, to another part of the pressure detected by the hydraulic pressure sensor channels changes in the situation in order to determine the liquid level detection needle. 当样本针接触液面准备吸样时,液路通道内部压力状况会突然变化,从而产生了一个明显的跳变,在吸样结束之后也将产生一个内部压力的突然变化,压力值恢复,这样,就形成了一个压力脉冲,通过对压力脉冲信号进行复杂的运算,并与经验值比较来判断吸液是否正常。 When contact with the liquid sample needle aspiration sample preparation, liquid channels inside pressure situation will suddenly change, resulting in a significant transition, after the end of the suction-like sudden changes will also have an internal pressure, the pressure is restored, so , to form a pressure pulse, the pressure pulse signal by complex calculations, and comparison with the experience to judge aspiration is normal. 因为正常信号与非正常信号脉宽不一样,所以可以由此来判断不正常的工作状态,如样本针堵塞等。 Because the normal signal and abnormal signal pulse width is not the same, so you can use that to determine abnormal operating condition, such as sample needle blockage. [0004] 上述用压力检测样本针是否堵塞的方法是目前解决样本针堵塞判断的有效途径, 而且压力检测也能同时实现液面检测功能。 [0004] The above-mentioned pressure test sample needle is blocked approach is an effective way to solve the sample needle blockage judgment, but also can realize the pressure sensing the liquid level detection function. [0005] 但是压力检测液面的方法易受干扰,因此,为了既能可靠地实现液面检测功能,又能实现液面检测针的堵塞判断,往往需要两种检测方法配合使用,结构较为复杂。 [0005] However, the method of pressure detection surface susceptible to interference, and therefore, in order to reliably achieve both the liquid level detection function, but also to achieve the level detecting needle clogging determination, often require two detection methods with the use of a more complex structure . 【发明内容】[0006] 本发明的目的在于提供一种生化分析仪样本针堵塞检测装置及方法,能够简便可靠地实现堵塞检测。 SUMMARY OF THE INVENTION [0006] The object of the present invention is to provide a biochemical analyzer sample needle clogging detection device and method capable of easily and reliably achieve blockage detection. [0007] 为实现上述目的,本发明提出的生化分析仪样本针堵塞检测装置包括样本针、检测电路,其特征是:所述样本针为可变阻抗;所述检测电路包括液面检测电路;所述样本针作为可变阻抗接入到液面检测电路中,液面检测电路检测所述可变阻抗的阻抗变化,并将检测信号输出。 [0007] To achieve the above object, the present invention provides a biochemical analyzer sample needle needle clogging detector means includes a sample, a detection circuit, characterized in that: said needle is variable impedance sample; said detection circuit comprises a level detection circuit; The sample needle as the access to the variable impedance level detection circuit, the impedance detection circuit detects changes in the liquid level of said variable impedance, and outputs a detection signal. [0008] 优选地,本装置所述可变阻抗为可变电容。 [0008] Preferably, the apparatus of the variable impedance is a variable capacitance. [0009] 优选地,本装置所述可变电容为双层同轴结构,内层与外层之间用绝缘材料隔绝, 内层和外层分别作为可变电容的两个极板。 [0009] Preferably, the variable capacitance means between the double-layer coaxial structure, inner and outer layers isolated with an insulating material, inner and outer layers, respectively, as the two plates of a variable capacitor. [0010] 优选地,本装置所述液面检测电路包括振荡源、仪表放大器和平衡电路,所述振荡源所产生的信号一路经平衡电路的第一支路输入到仪表放大器的负输入端,另一路经平衡电路的第二支路输入到仪表放大器的正输入端,样本针内外两层分别作为可变电容的两个极接入到平衡电路的第二支路中;所述平衡电路设定为当可变电容的电容值为特定值时, 其输入到仪表放大器的正输入端和负输入端的信号保持平衡,当可变电容的电容值偏离特定值时,其输入到仪表放大器的正输入端和负输入端的信号产生差异。 [0010] Preferably, the device according to the liquid level detection circuit includes an oscillation source, Amp and balanced circuit, the signal generated by the oscillation source input all the way through the first leg of the balanced circuit to the negative input of the instrumentation amplifier, another pass balanced circuit is inputted to a second branch of the positive input of the instrumentation amplifier, sample needle outer and inner layers, respectively, as the two variable capacitance pole balanced access to the second leg of the circuit; the balance of the circuit design When the capacitor is given a specific value when the variable capacitor, the input to the instrumentation amplifier positive input and negative input signal to maintain a balance, when the variable capacitor capacitance values deviate from the specific value, its positive input to the instrumentation amplifier input and the negative input signal differences. [0011] 优选地,本装置还包括控制板、上位机,所述控制板一端与检测电路相连,另一端与上位机相连;所述检测电路中还包括有依次相连的A/D变换电路、CPU、接口电路,所述A/ D变换电路的输入端与液面检测电路相连,所述接口电路的输出端与控制板的输入端相连。 [0011] Preferably, the apparatus further includes a control panel, the PC, the control panel is connected to one end of the detection circuit connected to the other end of the PC; the detection circuit also includes the consecutive A / D conversion circuit, input of CPU, interface circuit, said A / D conversion circuit and the liquid level detection circuit connected to the input terminal of said interface circuit output terminal is connected with the control panel. [0012] 优选地,本装置所述检测电路中还包括有温度传感电路,其输出端与A/D变换电路的另一输入端相连。 [0012] Preferably, the apparatus further comprises a detection circuit with a temperature sensing circuit, the other input terminal and the output terminal A / D converting circuit are connected. [0013] 本发明还包括一种生化分析仪样本针堵塞检测方法,其特征是包括如下步骤:A、 将样本针作为可变阻抗接入到液面检测电路中;B、利用检测电路检测所述可变阻抗的阻抗变化,并将检测信号输出;C、根据输出的信号判断样本针是否堵塞。 [0013] The present invention further comprises a biochemical analyzer sample needle clogging detecting method, characterized by comprising the steps of: A, the sample needle as the access to the variable impedance level detection circuit; B, using the detection circuit detects the changes in impedance of the variable impedance and output a detection signal; C, is determined based on the signal output from the sample needle is blocked. [0014] 优选地,本方法所述可变阻抗为可变电容。 [0014] Preferably, the method of the variable impedance is a variable capacitance. [0015] 优选地,本方法步骤B中检测电路检测所述可变阻抗的阻抗变化的方法包括如下步骤:B1、利用振荡源所产生信号;B2、将所述信号一路经平衡电路的第一支路输入到仪表放大器的负输入端,另一路经平衡电路的第二支路输入到仪表放大器的正输入端;B3、仪表放大器将检测到的信号输出;其中,样本针内外两层分别作为可变电容的两个极接入到平衡电路的第二支路中;所述平衡电路设定为当可变电容的电容值为特定值时,其输入到仪表放大器的正输入端和负输入端的信号保持平衡,当可变电容的电容值偏离特定值时,其输入到仪表放大器的正输入端和负输入端的信号产生差异。 [0015] Preferably, the impedance of the variable impedance variation of the method of the present method step B comprises detecting circuit detects the following steps: B1, the use of an oscillation signal generated by the source; B2, the signal of the first balanced circuit all the way through tributary input to the negative input of the instrumentation amplifier, another pass balanced input circuit of the second branch to the positive input of the instrumentation amplifier; B3, instrumentation amplifier outputs the detected signal; wherein the needle samples were used as outer and inner layers Two extremely variable capacitance access to the second leg of the balanced circuit; and when the balancing circuit is set when the variable capacitor capacitance value specific value, enter it into the amp's positive input and the negative input end signal to maintain a balance, when the capacitance value of the variable capacitance deviation particular value, the input to the positive input of the instrumentation amplifier and the negative input signal differences. [0016] 优选地,本方法在步骤B之后还包括如下步骤:将输出的检测信号依次输入到A/D 变换电路、CPU、接口电路、控制板、上位机。 [0016] Preferably, the method further comprising the step after step B: the detection signal is sequentially input to the A / D conversion circuit, CPU, interface circuits, control panels, PC. [0017] 优选地,本方法在步骤B中还检测环境温度,并将环境温度检测值依次输入到A/D 变换电路、CPU、接口电路、控制板、上位机。 [0017] Preferably, the method also detects the ambient temperature in step B, and the ambient temperature detection value sequentially input to the A / D conversion circuit, CPU, interface circuits, control panels, PC. [0018] 优选地,本方法将因堵塞造成的工作电压变化量按如下公式校正:[0020] 其中Vt为可变阻抗与温度的变化率,Tl为初始状态环境温度,Va为初始状态时液面检测电路工作电压,Vb为可变阻抗改变后的液面检测电路工作电压,T2为通过温度传感电路信号得到的Vb时的环境温度;当工作电压变化量大于设定的阈值则判断样本针堵塞。 [0018] Preferably, the method will work due to clogging of the voltage variation caused by the following formula Calibration: [0020] Vt wherein the variable rate of change of impedance and temperature, Tl ambient temperature to the initial state, Va is the initial state of the liquid face detecting circuit voltage, Vb is changed after the variable impedance level detection circuit voltage, T2 is a temperature sensing circuit by signal Vb obtained when the ambient temperature; when the operating voltage is greater than the set threshold value is determined Sample needle blockage. [0021] 与现有技术相比,本发明具有如下优点:[0022] 由于本发明将液面检测针作为一个广义的阻抗(优选为电容)接入到检测电路中,检测电路能够通过工作电压来计算当前可变电容(针电容)的大小,当液面检测针接触液面时其可变电容(针电容)会有较大的变化,同时,工作电压也会相应变化,并根据可变电容(针电容)的变化来判断液面检测针是否接触到了液面,因此,本发明能够利用电容液面检测方法能够可靠地实现液面检测功能,同时,不再需要对液路系统进行任何修改,不需要压力传感器及处理电路。 [0021] Compared with the prior art, the present invention has the following advantages: [0022] Since the present invention detects the liquid surface of the needle as a generalized impedance (preferably capacitive) access to the detection circuit, the detection circuit through the operating voltage to calculate the current variable capacitor (pin capacitance) in size, when the liquid level detection pin contacting surface of its variable capacitor (pin capacitance) vary greatly, while the operating voltage will change accordingly, and in accordance with the variable capacitance (capacitance needle) to determine whether changes in the liquid level detecting pins come into contact with the liquid surface, therefore, the present invention can use capacitive liquid level detection method can reliably detect the liquid level function, at the same time, eliminating the need for any hydraulic circuit system modification, no pressure sensors and processing circuits. 当液面检测针被样本中的纤维蛋白或血细胞等堵塞了,其可变电容(针电容)也会明显变化,配合当前环境温度值并能够判断液面检测针是否堵塞。 When the needle is a sample of the liquid level detection fibrin or blood cells clogged, with variable capacitance (pin capacitance) will be significant changes, with the current ambient temperature value and the ability to determine the liquid level detection pin is blocked. 因此,本发明利用电容液面检测的方法可靠地检测液面的同时,利用液面检测针本身电容的变化规律,能够简便可靠地实现堵塞检测。 Therefore, while taking advantage of the capacitive liquid level detection method of the present invention is to reliably detect the liquid level by the level variation of the capacitance detecting needle itself, can be realized easily and reliably detect clogging. [0023] 结合简单的温度传感电路,可使检测结果更加准确。 [0023] The combination of simple temperature sensor circuit, the detection result is more accurate. 【附图说明】[0024] 图1是本发明实施例生化分析仪样本针结构图;[0025] 图2是本发明实施例生化分析仪样本针被堵塞示意图;[0026] 图3是本发明实施例液面检测系统示意图;[0027] 图4是本发明实施例液面检测电路示意图;[0028] 图5是本发明实施例液面检测可变电容(针电容)随环境温度变化曲线。 BRIEF DESCRIPTION [0024] FIG. 1 is a biochemical analyzer sample needle diagram embodiment of the present invention; [0025] FIG. 2 is an embodiment of the invention biochemical analyzer sample needle is clogged schematic view; [0026] FIG. 3 is the present invention Example liquid level detection system schematic illustration; [0027] FIG. 4 is a schematic view of the liquid level detection circuit embodiment of the present invention; [0028] FIG. 5 is a curve with the ambient temperature Example level detecting variable capacitor (pin capacitance) embodiment of the present invention. 【具体实施方式】[0029] 实施例一[0030] 本方案是关于检测样本针堵塞的方法,利用了样本针堵塞后可变电容(针电容) 有较大变化的规律、可变电容(针电容)随环境温度变化的规律,以及可变电容(针电容) 与工作电压的一一对应关系来实现样本针的堵塞检测。 [DETAILED DESCRIPTION] [0029] Example A [0030] The program is about the test sample needle clogging method using a variable capacitor (pin capacitance) have a greater variation of the needle after the sample plug, variable capacitance (needle capacitance) law with changes in ambient temperature, and the correspondence between a variable capacitance (pin capacitance) and the operating voltage to achieve clogging test sample needle. 其中,我们采用了温度传感电路来指示当前温度,以便能够消除环境温度造成的对可变电容(针电容)的影响。 Among them, we have adopted a temperature sensing circuit to indicate the current temperature in order to eliminate the effects of ambient temperature caused by the variable capacitor (pin capacitor). [0031] 先看样本针4的结构,如图1所示,样本针4为双层同轴结构,内层如与外层4b 之间用绝缘材料4c隔绝,样本针4作为一个广义的可变电容接入到液面检测电路5中,内层如和外层4b分别作为可变电容(针电容)的两个非规则的极板。 [0031] Look at the sample needle 4 structure, shown in Figure 1, the sample 4 is double needle coaxial structure, such as an inner layer between the outer layers 4b and 4c isolated with an insulating material, the sample needle 4 can be generalized as a Variable capacitance access to the liquid level detecting circuit 5, such as the inner and outer plates 4b, respectively, as a variable capacitance (capacitance pin) of two non-regular. [0032] 根据电容器原理,电容器的电容量可以由电极面积S [m2],两极板间距离t[m]以及电介质的相对介电常数ε来表示,即[0033] C() ^ ε* (1)[0034]其中,ε 0 = 8. 85*10_12F/M,为一个常量。 [0032] According to the principle of a capacitor, the capacitance of the capacitor electrode area may be S [m2], the distance between the plates t [m] and an electrical relative permittivity ε of the medium is represented, i.e., [0033] C () ^ ε * (1) [0034] where, ε 0 = 8. 85 * 10_12F / M, is a constant. [0035] 因为样本针4结构已经固定,公式(1)中的ε与t并已确定,所以样本针4的电容量由电极面积S决定。 [0035] Because the structure of the sample needle 4 is fixed, equation (1), and ε and t are determined, so the sample needle 4 is determined by the capacitance of the electrode area S. 因空气的相对介电常数ε约为1,纯水的相对介电常数ε约为82,当样本针4接触样本杯10样本11液面后,可变电容(针电容)的一个极板边界发生了突变,相应地可以认为电极面积S发生了突变,因此样本针4接触液面后其可变电容(针电容)会有突然增大。 Because of the relative permittivity ε air is about 1, the relative dielectric constant ε pure water of about 82, when the sample needle 4 sample cup 10 contacts the liquid sample 11, a variable capacitor (pin capacitance) of a plate boundary mutated, and accordingly may be considered an electrode area S mutated, so the needle 4 after contact with the liquid sample its variable capacitor (pin capacitance) have suddenly increased. [0036] 样本针4吸取样本杯10中的样本11,可能造成样本针4堵塞,如图2所示,这种情况下,即使样本针4离开样本11液面处在空气中,因样本针4内层如的填充物发生改变, 使得可变电容(针电容)的一个电极的边界条件发生了改变,等同于电极面积S发生了改变,所以可变电容(针电容)同样会增大。 [0036] Sample 4 draw needle 10 in sample cup 11 in the sample, the sample may cause clogging of the needle 4, shown in Figure 2, in this case, even if the sample needle 4 is in the air leaving the liquid sample 11, because the sample needle 4 as an inner layer of filler is changed, so that the variable capacitor (capacitor needle) of one of the electrodes changed boundary conditions, equivalent to the electrode area S is changed, so that the variable capacitor (capacitor needle) also increases. [0037] 下面详细描述检测样本针堵塞的装置与方法。 [0037] Detailed device and method for detecting a sample needle clogging described below. [0038] 要检测样本针堵塞,必须要一个液面检测系统如图3所示,整个系统包括样本针4、检测电路1、控制板2、上位机3(如PC)等。 [0038] To test sample pin plug, must be a liquid level detection system shown in Figure 3, the system includes a sample needle 4, the detection circuit 1, the control board 2, PC 3 (such as PC) and the like. 检测电路1是功能实现的核心,它包括液面检测电路5、温度传感电路6、A/D变换7、CPU 8、接口电路9等。 1 is a core function of the detection circuit implementation, which includes level detection circuit 5, the temperature sensing circuit 6, A / D conversion 7, CPU 8, interface circuit 9 and the like. [0039] 液面检测电路5能够把样本可变电容(针电容)大小转化为当前工作电压,即工作电压与样本可变电容(针电容)是一一对应的,液面检测电路5的一种方案如图4所示, 信号源Vin分为两路分别输入到仪表放大器的两个输入端,Rl、Cl与R2、C2及样本可变电容(针电容)Cp组成平衡电路,信号Vin经Rl与Cl (平衡电路的第一支路)输入到仪表放大器的负输入端V-,信号Vin经R2、C2与可变电容(针电容)Cp (平衡电路的第二支路)输入到仪表放大器的正输入端V+,样本针4内外两层分别作为可变电容(针电容)Cp的两个极接入到电路中,其中外层4b接地,R3作为仪表放大器的增益调节电阻。 [0039] liquid level detection circuit 5 able to sample a variable capacitance (pin capacitance) size into the current working voltage, the operating voltage and the sample variable capacitor (pin capacitance) is one to one, a liquid level detection circuit 5 kinds of programs shown in Figure 4, the signal source Vin into two paths are input to the amp's two inputs, Rl, Cl and R2, C2 and sample variable capacitor (pin capacitance) Cp compositional balance circuit, the signal Vin by Rl and Cl (a first balanced circuit branch) is input to the negative input of the instrumentation amplifier V-, signal Vin by R2, C2 and the variable capacitance (pin capacitance) Cp (a second balanced circuit branch) is input to the instrument amplifier positive input V +, the sample needle 4 outer and inner layers, respectively, as the two variable capacitance (pin capacitance) Cp very access to the circuit in which the outer layer 4b ground, R3 as instrumentation amplifier gain adjustment resistor. 图4中其他标号为:51为振荡源,52为仪表放大器,53为电平变换电路。 4 other designated: 51 of the oscillator, 52-amp, 53 of level shift circuit. 所述平衡电路设定为当可变电容Cp的电容值为特定值时,其输入到仪表放大器52的正输入端V+和负输入端V-的信号保持平衡,当可变电容Cp的电容值偏离特定值时,其输入到仪表放大器52的正输入端V+和负输入端V-的信号产生差异。 The balancing circuit is set when the capacitance value of the specific value of the variable capacitance Cp when its input to the positive input of the instrumentation amplifier 52 and the negative input V + V- signal balance, when the capacitance value of the variable capacitance Cp When the deviation from the specified value, which is input to instrumentation amplifier 52 the positive input terminal V + and V- of the negative input signal difference. [0040] 当样本针接触液面使得可变电容(针电容)发生突变,引起仪表放大器输入端信号变化,从而使得输出信号Vo也发生突变,并通过A/D采样把模拟信号转换成数字信号,送入到CPU 8,经过信号处理后,通过接口电路9把液面检测信号13输出给控制板2。 [0040] When the sample contacting surface so that the variable capacitance pin (pin capacitance) is mutated, cause instrumentation amplifier input signal changes, so that the output signal Vo also mutate, and by A / D sampling the analog signal into a digital signal , fed to the CPU 8, after the signal processing circuit 9 through the interface level detection signal 13 is output to the control board 2. [0041] 图3中,温度传感电路6能够把当前的环境温度转换成相应电压值,也送入到CPU 8,通过CPU 8软件计算就能够得到当前的环境温度值。 [0041] FIG. 3, the temperature sensing circuit 6 to the current ambient temperature can be converted into a corresponding voltage value, it is fed to the CPU 8, by the CPU 8 can be calculated by the software on the current ambient temperature. [0042] 在常温如25C下,样本针清洗干净后处于某一个初始位置,未与液面接触,如生化分析仪清洗池上方,调整液面检测电路5,使得工作电压在一个合适的电平,设为Va,并能够计算对应的样本针4电容值。 [0042] at room temperature such as 25 C, the sample needle cleaned in a particular initial position not in contact with liquid, such as biochemical analyzer cleaning above the pool, adjust the liquid level detection circuit 5, so that the operating voltage in a suitable level, is set to Va, and be able to calculate the sample needle 4 corresponding capacitance values. 生化仪长时间运行后,可能造成样本针4堵塞,当样本针4 堵塞后,可变电容(针电容)明显增大,相应地,工作电压也会增大,设为Vb,在环境温度保持25C不变的情况下,当Vb-Va大于某一个阈值设为Δ V, CPU 8就通过接口电路9输出堵塞信号14给控制板2,表示样本针4被堵塞了。 After long-running biochemical analyzer, may cause clogging sample needle 4, when the sample needle 4 blockage, variable capacitor (pin capacitance) significantly increased, and accordingly, the operating voltage will increase, set to Vb, the temperature remains 25 C under the same circumstances, when a Vb-Va is larger than a threshold value is set to Δ V, CPU 8 outputs a congestion signal to the control panel 14 2, shows a sample needle 4 is blocked by the interface circuit 9. [0043] 控制板2收到堵塞信号之后,发信息通知上位机3 (如PC),要求上位机3从人机界面通知操作者样本针4堵塞了,由此操作者就能够采取对应措施,比如进行强制冲洗等。 After the [0043] control panel 2 receives the jam signal, a message notification PC 3 (such as PC), PC 3 requires notification to the operator from the sample needle 4 blocked interface, whereby the operator would be able to take countermeasures, For example, washing and other mandatory. 表1是具体的实验数据,样本针吸取一定量血清样本后静止放置,模拟针堵塞过程,模拟实验一中,样本针堵塞前后可变电容(针电容)分别为:25. 21pF、25. 63pF,变化量为0. 42pF, 堵塞前后工作电压分别为:0. 913VU. 862V,变化量为0. 949V ;模拟实验二中,样本针堵塞前后可变电容(针电容)分别为:25.86pF、26. 37pF,变化量为0. 51pF,堵塞前后工作电压分别为:1. 427V、2. 523V,变化量为1. 096V ;[0044] 表1样本针堵塞实验[0045] Table 1 is a specific experimental data, the sample needle still draw a certain amount of serum samples placed, a simulated needle plugging process, a simulation experiment, the variable capacitance (capacitance needle) around the sample needle clogging respectively:. 25 21pF, 25 63pF. the amount of change to 0. 42pF, working voltage before plugging were: 0 913VU 862V, variation is 0. 949V; simulation II, before the sample needle blockage variable capacitor (pin capacitance), respectively:.. 25.86pF, 26. 37pF, the amount of change of 0. 51pF, respectively before and after the blockage working voltage:.. 1 427V, 2 523V, the amount of change is 1. 096V; [0044] Table 1 Sample needle clogging experiment [0045]

Figure CN101004424BD00071

[0046] 由此可见,样本针堵塞前后可变电容(针电容)变化量以及工作电压变化量都较大。 [0046] Thus, before the sample needle blockage variable capacitor (pin capacitance) Operating voltage variation and variation are larger. [0047] 前面提到要求环境温度保持不变,这是因为样本可变电容(针电容)大小会随着环境温度变化而变化,两者近似线性关系,如图5所示,环境温度升高可变电容(针电容) 将增大,变化率约为0.03pF/C。 [0047] aforementioned requirements ambient temperature remains constant, this is because the sample variable capacitance (capacitance needle) size will change as the ambient temperature changes, the two approximately linear relationship, as shown, ambient temperature 5 variable capacitance (pin capacitance) will increase the rate of change is about 0.03pF / C. 其中,横坐标为环境温度(C),纵坐标为可变电容(针电容)值(PF)。 Wherein the abscissa is the ambient temperature ( C), the ordinate is a variable capacitance (pin capacitance) value (PF). 曲线标注为环境温度分别在3C、5C、13C、24C与35C条件下的可变电容(针电容)值。 Curve labeled as ambient temperatures at 3 C, 5 C, 13 C, 24 C and 35 C condition variable capacitance (pin capacitance) value. [0048] 根据工作电压与样本可变电容(针电容)的对应关系,环境温度升高将使得工作电压也增大,根据样本可变电容(针电容)与温度的变化率可以得到工作电压与温度的变化率,设为Vt/c。 [0048] According to the operating voltage and the sample variable capacitor (pin capacitance) of correspondence between the ambient temperature will make the operating voltage is increased, based on the sample variable capacitor (pin capacitance) and the rate of change of temperature and operating voltage can be obtained the rate of change of temperature, set Vt / c. [0049] 比如,在常温25C下,样本针处于初始位置时,工作电压为Va,当样本针堵塞后, 工作电压增大为Vb,如果环境温度升高到了30C,则工作电压应为:Vb+(30-2 *Vt。[0050] 这样就可能出现两种误判,一种是虽然样本针未堵塞,但因为环境温度的升高使得工作电压增大,当电压增大到一定程度,比如超过了前面提到的检测阈值Δν,则就会误判堵塞;另一种情况是,虽然样本针已经堵塞了,工作电压增大了,但因为环境温度降低使得工作电压又减小,这会使得工作电压变化不能超过检测阈值△ V,也同样会造成误判。[0051] 本方案中,利用温度传感电路6把当前的环境温度对应值输入到CPU 8,通过软件处理来解决温度的影响问题。假设样本针处于初始状态,即清洗干净且位于清洗池上方, 环境温度为Tl,此时液面检测电路工作电压为Va,当样本针堵塞等原因使得样本可变电容(针电容)增大,工作电压也升高为Vb,并通过温度传感电路信号得到此时的环境温度为T2,因此,除去环境温度影响外,因堵塞造成的工作电压变化量,用V表示,应为:[0052] When the [0049] For example, at room temperature at 25 C, the sample needle is in the initial position, the working voltage Va, when the sample pin plug, operating voltage increases to Vb, if the ambient temperature rises to 30 C, the operating voltage should be: Vb + (30-2 * Vt [0050] This may be two false positives, although one is a sample needle is not blocked, but because of the ambient temperature such that the operating voltage increases, when the voltage is increased to. to a certain extent, such as exceeding the detection threshold Δν mentioned earlier, the blockage will be false; another case is that, although the sample needle has been blocked, the operating voltage is increased, but because the ambient temperature is lowered so that the operating voltage and Save small, which would make working voltage can not exceed the detection threshold △ V, will also result in miscarriage of justice. [0051] The program, the use of temperature sensing circuit 6 current ambient temperature corresponds to the value input to CPU 8, by software processing to address the impact of temperature is assumed that the sample needle in the initial state, that is clean and is located above the pool cleaning, the ambient temperature is Tl, at this time the liquid level detection circuit voltage Va, when the sample needle blockage reasons that sample variable capacitance (pin capacitance) increases, the operating voltage also rises as Vb, and the ambient temperature obtained at this time by the temperature sensing circuit T2 signal, therefore, to remove the external ambient temperature, the amount of voltage change caused by clogging of the work, with V said should be: [0052]

Figure CN101004424BD00081

[0053] 当V大于Δ V,则判断样本针堵塞。 [0053] When V is greater than Δ V, it is determined that the sample needle clogging. [0054] 其更多实施例[0055] 上述实施例仅用于对本发明进行说明,而并非对本发明的保护范围的限制。 [0054] it further embodiments [0055] The above examples are only used to illustrate the invention and not to limit the scope of the present invention. 本领域的技术人员在本发明的启示下可以做出一些变通的设计,均属于本发明的保护范围,例如:[0056] 样本针也可以设计成可变电感、可变电阻等可变电阻抗,也可以设计成磁敏元件、 巨磁电阻等可变磁阻抗,等等;相应地,液面检测电路也可以改为电感检测电路、电阻检测电路、磁阻检测电路,等等,不再列举。 Those skilled in the art in light of the present invention can make some modifications to the design, it belongs to the scope of the present invention, for example: [0056] The sample needle can also be designed as a variable inductor, variable resistors and other variable impedance, may also be designed as magnetic elements, and other giant magnetoresistance variable magnetic impedance, and the like; accordingly, the liquid level detection circuit detecting circuit may be changed to inductance, resistance detection circuit, the detection circuit reluctance, and the like, without then enumerated.

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Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US970274916 Oct 201511 Jul 2017Shenzhen Mindray Bio-Medical Electronics Co., Ltd.Liquid surface detection method and device, and immunoassay analyzer
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Classifications
International ClassificationG01N35/10, G06F19/00, G01R31/00
Legal Events
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25 Jul 2007C06Publication
28 Nov 2007C10Request of examination as to substance
27 Apr 2011C14Granted