CN100507635C - Laser micro control device and method for transportation and orientation of movable corpuscle and cell - Google Patents
Laser micro control device and method for transportation and orientation of movable corpuscle and cell Download PDFInfo
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- CN100507635C CN100507635C CNB2007101647409A CN200710164740A CN100507635C CN 100507635 C CN100507635 C CN 100507635C CN B2007101647409 A CNB2007101647409 A CN B2007101647409A CN 200710164740 A CN200710164740 A CN 200710164740A CN 100507635 C CN100507635 C CN 100507635C
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Abstract
The present invention relates to a laser micro-control device and method for the transportation and directional movement of particles and cells. In the device, a light source system and a two-dimensional moving loading platform are arranged in the same plane. The light source system is a laser, a light beam converting system and a focusing system which are orderly placed. The laser, the light beam converting system and the focusing system are arranged on the same optical axis. The present invention utilizes the characteristic that laser beam has gradient force and radiation force to restrict particles and cells needing transportation and directional movement in a light beam focusing area, and can carry out the transportation of particles and cells through a single laser beam as well as the directional movement of particles and cells through two intersecting laser beams, so as to realize the method of controlling particles and cells. The present invention can utilize laser micro-control technology to realize the direct transportation and directional movement of particles and cells, and can be applied to micro-fluidic chemistry or biological analysis systems.
Description
Technical field
The present invention relates to laser technology field, relate in particular to a kind of laser micro control device and method that is used to transport with orientation of movable corpuscle and cell.
Background technology
Since utilizing the gradient force of laser and radiant force in 1970, Ashkin catches and controls the concern that glass granules has caused more and more people later on, particularly 1986 he invented the laser optical tweezer technology, hereafter the laser optical tweezer technology has obtained development rapidly, has become the effective means of catching and control particulate and cell.The wide range of the particle of being controlled simultaneously comprises neutral atom and molecule, and miniature medium bead and living cells or the like.
As everyone knows, light has energy and momentum, the therefore momentum of photon and energy is understood and mutual exchange can take place particle when photon and particle are had an effect, thus produced tonsure power and the scattering force of light to particle.And in the laser beam, because it has the characteristic of gradient force and scattering force, become the important method that use in the optical control field, as laser catheter, optical tweezers (light tweezer) and light spanner, and laser beam or hollow beam have a very wide range of applications in microscopic particle (as micro particles, nano particle, free electron, biological cell and atom or molecule etc.) accurate, contactless controlled and control.The control system device of traditional laser optical tweezer comprises laser instrument, strong focusing system, sample operation platform and microscopic observation system.
Traditional microfluidic analysis system is to particulate and cell transports and directed moving all is to add electrode at the container two ends that put particulate and cell, particulate and cell are polarized, polarization back particulate and cell will be subjected to the effect of the electric field force of two end electrodes, towards direction of an electric field transportation, thereby reach the effect that particulate and cell are transported.Can two pairs of electrodes, wherein 0＜θ＜180 be set according to predetermined angle θ simultaneously
0, can carry out orientation to particulate and cell according to two kinds of methods and move.First kind is that two pairs of electrodes are not the while making alives, earlier to first pair of electrode making alive, particulate and cell are polarized, particulate and cell towards the direction transportation of first pair of electric field, when arriving two pairs of electric field intersections, are closed first pair of electrode under the effect of electric field force, open second pair of electrode, particulate and cell can polarize under the effect of second pair of electrode, can carry out the directed effect that moves thereby reach to particulate and cell towards second pair of electrode direction transportation.Second kind is that two pairs of electrodes add voltage simultaneously, and the voltage of first pair of electrode is U1, and the voltage of second pair electrode is U2, wherein U2 〉=1.5 * U1.Earlier particulate or cell are placed in first pair of electrode; when particulate is transported to two pairs of electrode intersections under the effect of the electric field force of first pair of electrode; because the voltage of second pair of electrode is higher than first pair of voltage; therefore particulate can change original transporting direction under the effect of second pair of electrode; towards the direction transportation of second pair of electrode, thereby can realize equally the orientation of particulate is moved.These two kinds of methods can both realize moving with the orientation of bipolar electrode to particulate and cell.This method with electrode is also smaller for the influence of particulate, but then influences greatlyyer for cell, might influence cell tissue even destroy cell membrane if pair cell applies voltage.And the operation that the orientation of pair cell moves on biology is very many, therefore presses for to seek another to the littler or even undamaged control method of biological tissue's damage.
Summary of the invention
The objective of the invention is easily biological cell to be produced in order to solve traditional method and to transport the defective of damage with directed migratory cell with electrode, make full use of the characteristic of laser beam, a kind of laser micro control device and method that is used to transport with orientation of movable corpuscle and cell is provided.
A kind of laser micro control device that is used to transport with orientation of movable corpuscle and cell comprises laser instrument, Beam Transformation system, focusing system, two dimensional motion article carrying platform, microscopic observation system, two dimensional motion article carrying platform below is provided with microscopic observation system, light-source system is laser instrument, Beam Transformation system, the focusing system of placing successively, laser instrument, Beam Transformation system, focusing system are placed on the same optical axis, and light-source system and two dimensional motion article carrying platform are placed in the same plane.
Described light-source system is laser instrument, Beam Transformation system, the focusing system of placing successively, and laser instrument, Beam Transformation system, focusing system are placed on the same optical axis, and described light-source system is a plurality of.
A kind of laser micro control method that is used to transport with orientation of movable corpuscle and cell is: at first use microscopic observation system to select to be controlled the relatively zone of dense distribution of particulate and cell, with focusing system to the incoming laser beam compression of girdling the waist, its beam waist only is several times of diameter of particulate and cell, laser beam irradiation after the compression is gone into to be mounted with the container of particulate and cell, adjust the two dimensional motion article carrying platform, concrete steps are as follows:
1) open laser instrument 2 in the light-source system, laser instrument output Gaussian beam, or Gaussian beam obtained hollow beam by the Beam Transformation system 3 in the light-source system again;
2) laser beam with 4 pairs of outputs of the focusing system in the light-source system focuses on, and laser beam is compressed in 100 microns;
3) regulate the container that 7 pairs of microscopic observation systems are mounted with particulate and cell 5 and scan, find the most intensive zone to be controlled of particulate and cell distribution
4), will wait to control particulate and cell 5 moves to the laser radiation zone by two dimensional motion article carrying platform 6;
5) adjust the height of two dimensional motion article carrying platform 6, make and wait to control particulate or cell 5 is positioned at the laser beam waist position;
6) check the laser radiation zone with microscopic observation system 7, observation particulate or cell 5 are under the effect of laser beam, towards laser beam transmission direction campaign, by regulating laser power, realize the best travelling speed of particulate, thereby realize particulate and cell are transported;
The laser micro control method that another kind is used to transport with orientation of movable corpuscle and cell is: at first use microscopic observation system to select to be controlled the relatively zone of dense distribution of particulate and cell, with focusing system to the incoming laser beam compression of girdling the waist, make the laser beam of last transportation particulate and cell girdle the waist in micron dimension, its beam waist only is several times of diameter of particulate and cell, two bundle laser beam after the compression are gone into to be mounted with the container of particulate and cell with the θ angular illumination, wherein, 0＜θ＜180
0, adjust two-dimension moving platform, make laser beam irradiation in the zone that distributes than comparatively dense, concrete steps are as follows:
1) open laser instrument 2 in the light-source system, laser instrument output Gaussian beam, or Gaussian beam obtained hollow beam by the Beam Transformation system 3 in the light-source system again;
2) laser beam with 4 pairs of outputs of the focusing system in the light-source system focuses on, and laser beam is compressed in 100 microns;
3) regulate the container that 7 pairs of microscopic observation systems are mounted with particulate and cell 5 and scan, find the most intensive zone to be controlled of particulate and cell distribution
4), will wait to control particulate and cell 5 moves to the laser radiation zone by two dimensional motion article carrying platform 6;
5) adjust the height of two dimensional motion article carrying platform 6, make and wait to control particulate or cell 5 is positioned at the laser beam waist position;
6) check the laser radiation zone with microscopic observation system 7, observation particulate or cell 5 are under the effect of laser beam, towards laser beam transmission direction campaign, by regulating laser power, realize the best travelling speed of particulate, thereby realize particulate and cell are transported;
Particulate arrives two laser intersection position, turns off the first bundle laser beam, opens the second bundle laser beam, particulate is under the effect of the gradient force of the second bundle laser beam, its direction of motion changes, and will move along the second bundle Laser Transmission direction, realizes that the orientation that laser beam is controlled particulate moves;
The laser beam that perhaps with the first bundle light intensity is P1 earlier is facing to the particulate irradiation; particulate is under the effect of the gradient force of laser beam and radiant force; along the transmission direction transportation of the first bundle laser beam, when particulate arrival two laser intersection position, the light intensity of the second bundle laser is P2; wherein; P2 〉=1.5*P1, particulate can turn to, along the transmission direction campaign of the second bundle laser beam; make the direction of motion of particulate and cell change, realize that the orientation that laser beam is controlled particulate moves.
Principle of work of the present invention: when laser photon shines on transparent particulate bead or the cell, the photon of a part will be reflected, and its direction of motion can be followed the direction of reflection law, this direction is that certain variation is arranged with respect to the incident laser beam direction, in collision process, photon has energy and momentum, therefore the momentum of photon and energy is understood and mutual exchange will take place particle when photon and particle are had an effect, thereby produced the radiant force of light to particle, thus particulate and cell can be under the effect of radiant force along the transmission direction campaign of light.Simultaneously because the characteristics of laser beam in light distribution, particulate and cell will be subjected to the effect of the gradient force of laser beam, its direct effect is strapped in the laser radiation zone with particulate and cell exactly, thereby change the size of the gradient force of laser beam by the power of regulating laser, make the size of gradient force can overcome other extraneous perturbed forces such as Brownian movement power, so just can utilize this power carry out to particulate and cell transportation, operation such as move.Can adopt simultaneously double laser beam to come that particulate and cell are carried out orientation and move, the delay of the switch by regulating two bundle laser and regulate two and restraint size on the light intensity of laser and realize operations such as orientation to particulate and cell moves respectively.
The present invention can utilize the characteristic of the gradient force and the scattering force of laser beam or hollow beam, realization is to the transportation and directed the moving of particulate and cell, can overcome the not enough point of tradition, can realize biological cell is transported and directed research of moving with electrode method.
Description of drawings:
Fig. 1 is the synoptic diagram of laser photon to the light action power of particulate and cell, and F and F ' represent the acting force of 1 pair of particulate of focussed laser beam and cell 5 among the figure;
Fig. 2 is the little actuation means structural representation of laser that is used for orientation of movable corpuscle or cell: figure: particulate that 2-laser instrument, 3-Beam Transformation system, 4-focusing system, 5-are to be controlled or cell, 6-two dimensional motion article carrying platform, 7-microscopic observation system.
Embodiment
As shown in Figure 2, be used to transport laser micro control device with orientation of movable corpuscle and cell, comprise laser instrument 2, Beam Transformation system 3, focusing system 4, two dimensional motion article carrying platform 6, microscopic observation system 7, two dimensional motion article carrying platform 6 belows are provided with microscopic observation system 7, light-source system is laser instrument 2, Beam Transformation system 3, the focusing system 4 of placing successively, laser instrument 2, Beam Transformation system 3, focusing system 4 are placed on the same optical axis, and light-source system and two dimensional motion article carrying platform 6 are placed in the same plane.
Described light-source system is laser instrument 2, Beam Transformation system 3, the focusing system 4 of placing successively, and laser instrument 2, Beam Transformation system 3, focusing system 4 are placed on the same optical axis.Light-source system is a plurality of.Wherein laser instrument emission laser beam pattern is the Gaussian beam of basic mode, and moving of two dimensional motion article carrying platform can be manually or the mode of motion of employing electro-motor, and microscopic observation system can be chosen as the microscopic system that can show in real time with online observation.
The effect of laser instrument 2 is emission LASER Light Source, its outgoing beam is a fundamental-mode gaussian beam, the effect of Beam Transformation system 3 is that the solid Gaussian beam with laser emitting is converted to hollow beam, the effect of focusing system 4 is that the with a tight waist of outgoing beam compressed, with reach the experiment demand with a tight waist be about 1-100 microns light beam, the effect of two dimensional motion article carrying platform 6 is to put particulate and cell 5 and control its direction of motion, and the effect of microscopic observation system 7 is auxiliary accurately location and online observations.
A kind of laser micro control device and method that is used to transport with orientation of movable corpuscle and cell, it is characterized in that: at first use microscopic observation system to select to be controlled the relatively zone of dense distribution of particulate and cell, with focusing system to the incoming laser beam compression of girdling the waist, its beam waist only is several times of diameter of particulate and cell, laser beam irradiation after the compression is gone into to be mounted with the container of particulate and cell, adjust the two dimensional motion article carrying platform, concrete steps are as follows:
1) open laser instrument 2 in the light-source system, laser instrument output be solid Gaussian beam, control particulate and cell with hollow beam if desired, only need just can obtain hollow beam with Gaussian beam again by the Beam Transformation system 3 in the light-source system;
2) laser beam with 4 pairs of outputs of the focusing system in the light-source system focuses on, laser beam is compressed in 100 microns, because directly the solid light beam or the hollow beam of output generally are several millimeters magnitudes, and general magnitude at the 1-100 micron of the diameter magnitude of particulate of being controlled and cell, therefore be necessary very much the compression of girdling the waist of the solid light beam of incident or hollow beam, be compressed in 100 microns, thereby obtain testing laser beam or the hollow beam that needs;
3) regulate the container that 7 pairs of microscopic observation systems are mounted with particulate and cell 5 and scan, find the most intensive zone to be controlled of particulate and cell distribution, control with particulate and cell that laser beam is controlled in the zone intensive waiting, this can improve controls efficient;
4) by two dimensional motion article carrying platform 6, to wait to control particulate and cell 5 moves to the laser radiation zone, in the experiment with laser beam rough alignment zone to be controlled, keep laser beam motionless, regulate the manual or electro-motor of two dimensional motion article carrying platform, can accurately particulate and cell be moved to the laser beam irradiation zone like this;
5) height of adjustment two dimensional motion article carrying platform 6, make and wait to control particulate or cell 5 is positioned at the laser beam waist position, laser beam is in the light intensity maximum of beam waist position, and the gradient force that laser beam produces when acting on particulate with cell is relevant with light intensity intensity with radiant force, therefore the beam waist position of laser beam need be aimed at particulate and cell;
6) check the laser radiation zone with microscopic observation system 7, observation particulate or cell 5 are under the effect of laser beam, towards laser beam transmission direction campaign, by regulating laser power, select and be fit to the transmission speed that microscopic observation system 7 can be observed, realize the best travelling speed of particulate, thereby realize particulate and cell 5 are carried out the single beam transportation;
Another kind is used for the little control method of laser of orientation of movable corpuscle or cell; it is characterized in that: at first use microscopic observation system to select to be controlled the relatively zone of dense distribution of particulate and cell; with focusing system to the incoming laser beam compression of girdling the waist; make the laser beam of last transportation particulate and cell girdle the waist in micron dimension; its beam waist only is several times of diameter of particulate and cell; two bundle laser beam after the compression are gone into to be mounted with the container of particulate and cell, wherein 0＜θ＜180 with the θ angular illumination
0, adjust two-dimension moving platform, make laser beam irradiation in the zone that distributes than comparatively dense, concrete steps are as follows:
1) open laser instrument 2 in the light-source system, laser instrument output be solid Gaussian beam, control particulate and cell with hollow beam if desired, only need just can obtain hollow beam with Gaussian beam again by the Beam Transformation system 3 in the light-source system;
2) laser beam with 4 pairs of outputs of the focusing system in the light-source system focuses on, laser beam is compressed in 100 microns, because directly the solid light beam or the hollow beam of output generally are several millimeters magnitudes, and general magnitude at the 1-100 micron of the diameter magnitude of particulate of being controlled and cell, therefore be necessary very much the compression of girdling the waist of the solid light beam of incident or hollow beam, be compressed in 100 microns, thereby obtain testing laser beam or the hollow beam that needs;
3) regulate the container that 7 pairs of microscopic observation systems are mounted with particulate and cell 5 and scan, find the most intensive zone to be controlled of particulate and cell distribution, control with particulate and cell that laser beam is controlled in the zone intensive waiting, this can improve controls efficient;
4) by two dimensional motion article carrying platform 6, to wait to control particulate and cell 5 moves to the laser radiation zone, in the experiment with laser beam rough alignment zone to be controlled, keep laser beam motionless, regulate the manual or electro-motor of two dimensional motion article carrying platform, can accurately particulate and cell be moved to the laser beam irradiation zone like this;
5) height of adjustment two dimensional motion article carrying platform 6, make and wait to control particulate or cell 5 is positioned at the laser beam waist position, laser beam is in the light intensity maximum of beam waist position, and the gradient force that laser beam produces when acting on particulate with cell is relevant with light intensity intensity with radiant force, therefore the beam waist position of laser beam need be aimed at particulate and cell;
6) check the laser radiation zone with microscopic observation system 7, observation particulate or cell 5 are under the effect of laser beam, towards laser beam transmission direction campaign, by regulating laser power, select and be fit to the transmission speed that microscopic observation system 7 can be observed, realize the best travelling speed of particulate, thereby realize particulate and cell 5 are carried out the single beam transportation;
Particulate arrives two laser intersection position, turns off the first bundle laser beam, opens the second bundle laser beam, particulate is under the effect of the gradient force of the second bundle laser beam, its direction of motion changes, and will move along the second bundle Laser Transmission direction, realizes that the orientation that laser beam is controlled particulate moves;
The laser beam that perhaps with the first bundle light intensity is P1 earlier is facing to the particulate irradiation; particulate is under the effect of the gradient force of laser beam and radiant force; along the transmission direction transportation of the first bundle laser beam, when particulate arrival two laser intersection position, the light intensity of the second bundle laser is P2; wherein; P2 〉=1.5P1, particulate can turn to, along the transmission direction campaign of the second bundle laser beam; make the direction of motion of particulate and cell change, realize that the orientation that laser beam is controlled particulate moves.
Fundamental diagram of the present invention is referring to Fig. 1, Fig. 1 is the synoptic diagram of laser beam to the photodynamic action of particulate and cell, according to above-mentioned principle of work as can be known, the laser beam that focuses on is because the characteristic of its light distribution, when shining on particulate and the cell 5, laser beam 1 can in vertical and transmission direction, produce gradient force, the effect of gradient force makes particulate and cell be bound in the rayed zone, but same particulate and cell can carry out the exchange of momentum and energy with light beam, particulate and cell can be subjected to the effect of laser beam to the radiant force of its generation, pushing away particulate and cell and on optical transmission direction, moving, thereby realizing with of the transportation of single laser beam to particulate and cell.Can adopt laser beam equally, utilize the delay of switch of two light beams and the size of light intensity, can realize equally the orientation of particulate and cell is moved.
The present invention not only is applicable to the transparent particulate of micron and sub-micrometer scale, and is equally applicable to the transparent biological tissues such as biological cell of laser beam with the transportation and the directed micro-control method and the device that moves of laser beam to particulate and cell.
Claims (5)
1. laser micro control device that is used to transport with orientation of movable corpuscle and cell, it is characterized in that comprising laser instrument 2, Beam Transformation system 3, focusing system 4, two dimensional motion article carrying platform 6, microscopic observation system 7, two dimensional motion article carrying platform 6 belows are provided with microscopic observation system 7, light-source system is laser instrument 2, Beam Transformation system 3, the focusing system 4 of placing successively, laser instrument 2, Beam Transformation system 3, focusing system 4 are placed on the same optical axis, and light-source system and two dimensional motion article carrying platform 6 are placed in the same plane.
2. a kind of laser micro control device that is used to transport with orientation of movable corpuscle and cell according to claim 1 is characterized in that described light-source system can be for a plurality of.
3. a kind of laser micro control device that is used to transport with orientation of movable corpuscle and cell according to claim 1 is characterized in that the described laser beam of controlling particulate and cell can also can be hollow beam for the solid light bundle.
4. a use is installed according to claim 1 is used to transport laser micro control method with orientation of movable corpuscle and cell, it is characterized in that: at first use microscopic observation system to select to be controlled the relatively zone of dense distribution of particulate and cell, with focusing system to the incoming laser beam compression of girdling the waist, its beam waist is compressed in 100 microns, laser beam irradiation after the compression is gone into to be mounted with the container of particulate and cell, adjust the two dimensional motion article carrying platform, concrete steps are as follows:
1) open laser instrument 2 in the light-source system, laser instrument output Gaussian beam, or Gaussian beam obtained hollow beam by the Beam Transformation system 3 in the light-source system again;
2) laser beam with 4 pairs of outputs of the focusing system in the light-source system focuses on, and laser beam is compressed in 100 microns;
3) regulate the airtight container that 7 pairs of microscopic observation systems are mounted with particulate and cell 5 and scan, find the most intensive zone to be controlled of particulate and cell distribution;
4), will wait to control particulate and cell 5 moves to the laser radiation zone by two dimensional motion article carrying platform 6;
5) adjust the height of two dimensional motion article carrying platform 6, make and wait to control particulate or cell 5 is positioned at the laser beam waist position;
6) check the laser radiation zone with microscopic observation system 7, observation particulate or cell 5 towards laser beam transmission direction campaign, by the adjusting laser power, thereby are realized particulate and cell are transported under the effect of laser beam.
5. a use is installed according to claim 1 is used to transport laser micro control method with orientation of movable corpuscle and cell, it is characterized in that: at first use microscopic observation system to select to be controlled the relatively zone of dense distribution of particulate and cell, with focusing system to the incoming laser beam compression of girdling the waist, make the laser beam of last transportation particulate and cell girdle the waist in micron dimension, its beam waist is compressed in 100 microns, two bundle laser beam after the compression are gone into to be mounted with the container of particulate and cell with the θ angular illumination, wherein, 0＜θ＜180 °, adjust two-dimension moving platform, make laser beam irradiation in the zone that distributes than comparatively dense, concrete steps are as follows:
1) open laser instrument 2 in the light-source system, laser instrument output Gaussian beam, or Gaussian beam obtained hollow beam by the Beam Transformation system 3 in the light-source system again;
2) laser beam with 4 pairs of outputs of the focusing system in the light-source system focuses on, and laser beam is compressed in 100 microns;
3) regulate the container that 7 pairs of microscopic observation systems are mounted with particulate and cell 5 and scan, find the most intensive zone to be controlled of particulate and cell distribution;
4), will wait to control particulate and cell 5 moves to the laser radiation zone by two dimensional motion article carrying platform 6;
5) adjust the height of two dimensional motion article carrying platform 6, make and wait to control particulate or cell 5 is positioned at the laser beam waist position;
6) check the laser radiation zone with microscopic observation system 7, observation particulate or cell 5 towards laser beam transmission direction campaign, by the adjusting laser power, thereby are realized particulate and cell are transported under the effect of laser beam;
Particulate arrives two laser intersection position, turn off the first bundle laser beam, open the second bundle laser beam, particulate is under the effect of the second bundle gradient force of laser beam and radiant force, its direction of motion changes, to move along the second bundle Laser Transmission direction, realize that the orientation that laser beam is controlled particulate moves;
The laser beam that perhaps with the first bundle light intensity is P1 earlier is facing to the particulate irradiation; particulate is under the effect of the gradient force of laser beam and radiant force; along the transmission direction transportation of the first bundle laser beam, when particulate arrival two laser intersection position, the light intensity of the second bundle laser is P2; wherein; P2 〉=1.5 * P1, particulate can turn to, along the transmission direction campaign of the second bundle laser beam; make the direction of motion of particulate and cell change, realize that the orientation that laser beam is controlled particulate moves.
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| CNB2007101647409A CN100507635C (en) | 2007-12-12 | 2007-12-12 | Laser micro control device and method for transportation and orientation of movable corpuscle and cell |
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| CNB2007101647409A CN100507635C (en) | 2007-12-12 | 2007-12-12 | Laser micro control device and method for transportation and orientation of movable corpuscle and cell |
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| CN102181361B (en) * | 2011-03-25 | 2013-10-02 | 哈尔滨工业大学（威海） | Device and method for sorting cells |
| CN103897981B (en) * | 2014-04-03 | 2015-06-03 | 河北工业大学 | Cell screening device |
| KR101681422B1 (en) * | 2015-07-31 | 2016-11-30 | 가톨릭대학교 산학협력단 | Apparatus for analyzing cells using multi laser |
| CN105618166B (en) * | 2015-12-18 | 2017-05-10 | 武汉大学 | Equipment for realizing sorting of metal nanoparticles by utilizing Fano interference based light scattering force |
| GB201810010D0 (en) * | 2018-06-19 | 2018-08-01 | Imperial Innovations Ltd | Single particle automated raman trapping analysis |
| CN109633858B (en) * | 2019-02-19 | 2020-07-07 | 浙江大学 | Device and method for aligning focal points of correlation light beams in optical tweezers |
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