Search Images Maps Play YouTube News Gmail Drive More »
Sign in
Screen reader users: click this link for accessible mode. Accessible mode has the same essential features but works better with your reader.

Patents

  1. Advanced Patent Search
Publication numberCN100472197 C
Publication typeGrant
Application numberCN 200380106536
PCT numberPCT/DK2003/000877
Publication date25 Mar 2009
Filing date15 Dec 2003
Priority date20 Dec 2002
Also published asCA2507960A1, CA2507960C, CA2508000A1, CA2508001A1, CA2508070A1, CA2508113A1, CA2508323A1, CA2508323C, CA2508370A1, CA2511032A1, CA2974221A1, CN1726275A, CN1726386A, CN1955711A, CN100424166C, EP1572979A1, EP1572979A4, EP1573297A1, EP1573311A2, EP1573311A4, EP1573312A2, EP1573312A4, EP1573408A2, EP1573408A4, EP1576376A2, EP1576376A4, EP1579190A1, EP1579190A4, EP1579194A2, EP1579194A4, US7400983, US7648678, US7758809, US7937228, US7960178, US8216512, US8257968, US8298815, US8386195, US8394635, US8529836, US8663978, US8673642, US8784735, US8788217, US8969086, US9040284, US9229016, US9599630, US9778273, US20040266015, US20050064535, US20060045806, US20060046298, US20060063265, US20060085140, US20060088928, US20060088940, US20060172426, US20080241876, US20100081167, US20110167930, US20110269238, US20120310422, US20130029409, US20130084567, US20130203103, US20130217108, US20130330252, US20140038232, US20140186218, US20140234170, US20140286838, US20140356935, US20160084862, WO2004057307A1, WO2004058404A2, WO2004058404A3, WO2004058950A1, WO2004059284A2, WO2004059284A3, WO2004059287A2, WO2004059287A3, WO2004059288A2, WO2004059288A3, WO2004059297A1, WO2004059441A2, WO2004059441A3
Publication number200380106536.1, CN 100472197 C, CN 100472197C, CN 200380106536, CN-C-100472197, CN100472197 C, CN100472197C, CN200380106536, CN200380106536.1, PCT/2003/877, PCT/DK/2003/000877, PCT/DK/2003/00877, PCT/DK/3/000877, PCT/DK/3/00877, PCT/DK2003/000877, PCT/DK2003/00877, PCT/DK2003000877, PCT/DK200300877, PCT/DK3/000877, PCT/DK3/00877, PCT/DK3000877, PCT/DK300877
Inventors本诺·古根海默, 杰夫·劳, 约翰·法武齐, 罗桑奈·伟谢尔, 马克·基, 鲍勃·莱思罗普
Applicant达可斯通(丹麦)公司
Export CitationBiBTeX, EndNote, RefMan
External Links: SIPO, Espacenet
Method and apparatus for pretreatment of biological samples
CN 100472197 C
Abstract  translated from Chinese
本发明涉及一种通过按照染色规程依次施加预定量的试剂,而对容纳在载体例如显微镜载玻片(7)上的至少一种生物样品进行自动染色的方法和装置,其中至少一个载体配备在载体轨道组件中,载体轨道组件包括在载体配备到载体轨道组件中之后,用于预处理载体上的生物样品的装置,所述用于预处理生物样品的装置包括配备在载体轨道组件(6)中的罐(101);载体轨道(61);以及用于将配备在载体轨道(61)中的载体(7)绕枢轴旋转到垂直位置的装置,和用于将垂直载体(7v)浸渍到罐(101)内的装置。 The present invention relates to a staining procedure is applied in sequence according to a predetermined amount of a reagent, and a method and apparatus for receiving at least one biological sample carrier such as automatic staining microscope slides (7), with at least one carrier carrier track assembly, the track assembly comprises a carrier after the carrier with the carrier track assembly of the means for pre-treatment of biological samples on a support, said means for pre-treatment of biological samples with a carrier comprising a track assembly (6) The tank (101); a carrier rail (61); and means for the carrier with a track (61) in the carrier (7) pivoted to a vertical position and means for the vertical vector (7v) impregnated to the tank (101) within the apparatus. 通过将载体(7)从水平位置绕枢轴旋转到垂直位置,提供了一种在自动染色装置内完成预处理的自动方法和装置。 By the carrier (7) is pivoted from the horizontal position to the vertical position, there is provided a method and apparatus for the automatic completion of preprocessing in the automatic staining apparatus. 载体(7)的绕枢轴旋转确保载体在预处理和染色过程中的适当取向。 Vector (7) pivoting ensure proper orientation vector in the pretreatment and dyeing processes.
Claims(25)  translated from Chinese
1、一种对容纳在自动染色装置中的载玻片(7)上的至少一个生物样品进行处理的方法,所述方法包括以下步骤:在预定的载玻片部位配置一个承载生物样品的载玻片(7),所述载玻片(7)配置在载玻片固定器(62)中的基本水平的位置上;其特征在于:通过将所述的载玻片固定器(62)的枢轴旋转装置从一基本水平的载玻片位置旋转到一基本垂直的载玻片位置从而将所述载玻片(7)绕枢轴旋转到基本上垂直的位置;将所述基本上垂直取向的载玻片(7v)在处理罐(101)内浸渍一段预定的处理时间;在预定的处理时间以后从处理罐(101)移去垂直定位的载玻片(7v),以及通过将所述的载玻片固定器(62)的枢轴旋转装置从所述基本水平的载玻片位置旋转到所述基本垂直的载玻片位置,从而使所述的载玻片(7v)绕枢轴旋转到基本水平的位置。 1. A method for the automatic staining apparatus slides housed in at least one of the biological sample is processed (7), the method comprising the steps of: at a predetermined portion of the configuration of a slide carrier carrying biological sample slide (7), said slides (7) disposed on a slide glass holder (62) in a substantially horizontal position; characterized in that: the slide by the holder (62) pivoting means slides from a substantially horizontal position to a substantially vertical position so as to slide said slides (7) pivoted to a substantially vertical position; said substantially vertical Orientation slides (7v) in the treatment tank (101) within a predetermined period of processing time dipping; after a predetermined processing time from the processing tank (101) Remove vertical positioning slides (7v), as well as by the said slide holder (62) of the pivoting means from the substantially horizontal position to a slide of the slide substantially vertical position, so that the slide (7v) about a pivot axis to a substantially horizontal position.
2、 根据权利要求1所述的方法,其特征在于,所述载玻片部位是载玻片架,其中多,玻片(7)配置在载玻片架组件(6)内,而载玻片(7)可M所述载玻片固定器(62)的戶/M枢轴旋,置^^虫绕枢轴旋转。 2. The method according to claim 1, characterized in that the slides are slides frame portion, wherein multiple, slide (7) arranged on a slide glass holder assembly (6), and the slide glass piece (7) M said slides holder (62) indoor / M pivot rotation, set ^^ insect pivot.
3、 根据权利要求1或2戶脱的方法,其特征在于,所述的枢轴旋转装置包括可枢轴旋转的载玻片夹(69),其可以通过启动装置(67)在水平载玻片位置和垂直载玻片位置之间枢轴旋转。 3. The method of claim 1 or 2 removal claims, characterized in that said pivoting means comprises a pivotable slide clamp (69), which can be started by means (67) in a horizontal slide glass pivoting position between the sheet and the position of the vertical slides.
4、 根据权利要求1或2所述的方法,其特征在于,还包括将所选择的处理液从至少一个供应罐(111)供给到所述处理罐(101)内的步骤。 4. The method according to claim 1 or claim 2, characterized in that it further comprises the treatment liquid selected from at least one supply tank (111) is supplied to said processing tank (101) inside.
5、 根据^^利要求1或2所述的方法,其特征在于,还包括±真充和排空处理罐(101) 的步骤。 5, according to claim 1 or 2, wherein ^^ claims, characterized in that, further comprising filling and emptying ± true treatment tank (101) step.
6、 根据权利要求5所述的方法,其特征在于,液体从供应罐(111)转移到转移罐(110)并向上到达处理罐(101)。 6. A method according to claim 5 or claim, characterized in that the liquid transfer from a supply tank (111) into the transfer tank (110) to reach the treatment tank (101).
7、 根据权利要求1或2所述的方法,其特征在于,还包括使浸渍的载玻片(7)经受一系列的液体,戶腿液体依次填充处理罐(101)并从处理罐(101)排空。 7. The method according to claim 1 or claim 2, characterized in that, further comprising impregnating the glass slide (7) is subjected to a series of liquid, the liquid filled sequentially households legs treatment tank (101) and from the processing tank (101 ) emptying.
8、 根据权利要求i或2戶;M的方法,其特征在于,还包括将处理罐aoi)中含有的液体加热到预定?鹏的步骤,加热到至少6crc的升高aft。 8, according to claim i or 2; M method, characterized by further comprising treatment tank aoi) contained in the liquid is heated to a predetermined step Peng, heated to at least 6crc rise aft?.
9、 根据权利要求2所述的方法,其特征在于,还包括通过降低载玻片架(61)由此将至少一个垂直设置的载玻片(7v)插入到处理罐(101)内,而将至少一个垂直载玻片(7v)浸渍到处理罐(101)内的步骤。 9. The method according to claim 2, characterized by further comprising reducing glass slide carrier (61) whereby at least one vertically arranged slides (7v) inserted into the treatment tank (101), and at least one vertical slides (7v) impregnating treatment tank to the step (101) inside.
10、 根据权利要求1或2所述的方法,其特征在于,所述的生物样品处理方法是生物样品的预处理。 10. A method according to claim 1 or claim 2, characterized in that the processing method of the biological sample is pretreated biological sample.
11、 根据权利要求1或2戶腿的方法,其特征在于,生物样品是组织,戶腿的组织处理方法是组织样品的脱蜡处理。 11, according to claim 1 or claim 2 legs, characterized in that the biological sample is a tissue, tissue processing method of households legs dewaxing treatment is a tissue sample.
12、 根据权利要求1或2戶脱的方法,其特征在于,戶脱的生物样品的处理方法是生物样品的靶挽回处理。 12. The method of claim 1 or 2 removal claims, characterized in that the treatment of the biological sample households off target biological sample is treated to recover.
13、 根据权利要求1或2所述的方法,其特征在于,戶腿的处理方飽括短期储存下降到处理罐(101)中的液体内的垂直位置的载玻片(7v)上的样品。 13. A method according to claim 1 or claim 2, characterized in that the door handle side comprises short-term storage of full leg down to the vertical position of the processing liquid within the tank (101) in the slide (7v) on the sample .
14、 一种ffiil按照染色规程依次施加预定量的试剂,而对容纳在载玻片(7)上的至少一个生物样品进行自动染色的装置,其中一个载玻片(7)配备在载玻片架(61) 中的载玻片固定器(62)的基本水平的位置,辦征在于,载玻片架(61)被配备在一个载玻片架组件(6)中,载玻片架组件(6)包括在载玻片(7)被皿在载玻片固定器(62)中之后,用于预处理载玻片(7) 上的生物样品的装置,所述的用于预处理生物样品的装置包括配备在载玻片架组件(6)中的处理罐(101);用于将配备在载玻片固定器(62)中的载玻片(7)绕枢轴旋转到垂直载玻片位置的枢轴旋转装置,以及用于将垂直载玻片(7)浸渍到处理罐(101)内的装置; 以及使垂直载玻片(7v)枢轴旋转到基本7jC平的位置的装置。 14. A staining procedure is applied sequentially according ffiil predetermined amount of reagent, and at least one biological sample accommodated in the slides (7) on the automatic staining apparatus, wherein a glass slide (7) is provided in the slides frame (61) slides holder (62) a substantially horizontal position, do characterized in that slides rack (61) which is provided in a glass slide frame assembly (6), slides holder assembly (6) comprises slides (7) after being in the dish holder slides (62), means for preprocessing the biological sample slides (7), said means for preprocessing the biological The apparatus comprises a sample holder assembly with the slides (6) in the treatment tank (101); means for fixing with the slides (62) in the slide (7) pivoted to a vertical load slide means pivoting position, and means for vertical slides (7) immersed in the treatment tank means (101); and the vertical slides (7v) pivoted to a position substantially flat 7jC devices.
15、 根据权利要求14戶脱的装置,其特征在于,所述预处理载玻片(7)的装置包括,用于将载玻片架(61)的垂直设置的载玻片(7v)下降到处理罐(101)内的装置(63)。 15, removal apparatus according to claim 14, characterized in that said pretreatment slides (7) comprising means for the slides slides holder (61) is vertically arranged (7v) decreased to the processing tank unit (101) (63).
16、 根据权利要求14或15所述的装置,其特征在于,处理罐(101)是具有上开槽(102)的加长容器,所述槽使垂直取向的载玻片(7v)插入处理罐(101)内用于处理。 16. The apparatus according to claim 14 or 15, characterized in that the treatment tank (101) is a container having an upper elongated slot (102), so that said groove slides vertically oriented (7v) inserted into the treatment tank for processing (101).
17、 根据权利要求14或15所述的装置,其特征在于,所述处理罐(101)酉B有用于加热处理罐(101)内所含有的处理液的加热装置(104)。 17. The apparatus according to claim 14 or 15, characterized in that said processing tank (101) has a unitary B for heating the treatment tank (101) contained within the treatment liquid heating means (104).
18、 根据权利要求17所述的装置,其特征在于,所述加热装置(104)能够将处理罐(101)的内容物加热到至少6(TC的升高温度。 18. The apparatus of claim according to claim 17, characterized in that said heating means (104) capable of treatment tank (101) of the contents are heated to at least 6 (TC elevated temperatures.
19、 根据权利要求14或15任一项所述的装置,其特征在于,所述装置包括气动装置(113),所述气动装置将处理、^/人供应罐(111)转移到转移罐(110),从转移罐(110)转移到处理罐(101),以及将液体从处理罐(101)排到转移罐,从所述转移罐排到废物收集罐(112)。 19. The apparatus according to claim 14 or any one of claim 15, characterized in that said means comprises a pneumatic means (113), said pneumatic means processing, ^ / person supply tank (111) to a transfer tank ( 110), transferred from the transfer tank (110) to the processing tank (101), and the liquid is discharged from the processing tank transfer tank (101), discharged waste collection tank (112) from the transfer tank.
20、 根据权利要求14或15所述的装置,其特征在于,所述装置包括用于循环排出液的装置,循环排出、M新用于载玻片的随后预处理过程。 20. The apparatus according to claim 14 or 15, characterized in that said apparatus comprises means for circulating a liquid discharging, the discharge cycle, M new pretreatment for subsequent slides.
21、 根据权利要求14或15戶皿的装置,其特征在于,所述载玻片架组件(6)配备在抽屉组件中,其中载玻片架(61)可以从所述自动染色装置中缩回,以便装载和卸载载玻片(7)。 21, 14 or 15 according to the dish apparatus as claimed in claim wherein said frame assembly slides (6) with the drawer assembly in which the rack slides (61) can be reduced from the automatic staining apparatus back to the loading and unloading slides (7).
22、 根据权利要求21戶腿的装置,其特征在于,所述抽屉组件与处理罐(皿)配合,所述处理罐(101)倉,同时处理容纳在载玻片架组件(6)内的多个载玻片固定器(62)中的多个载玻片(7)。 22. The apparatus according to claim legs 21, characterized in that said drawer assembly with a treatment tank (dish) with said processing tank (101) positions, accommodated in the handle frame assembly slides (6) inside a plurality of slide glass holder (62) in a plurality of slides (7).
23、 根据权利要求22所述的装置,其特征在于,多个抽屉组件配备在所皿置内, 每个抽屉组件与处理罐(101)、至少一个转移罐(110)、供应罐(111)和废物罐(112) 配合。 23. The apparatus according to claim according to claim 22, characterized in that the drawer assembly with a plurality of the dish is set in each processing tank drawer assembly (101), at least one transfer tank (110), supply tank (111) and waste tank (112) complexes.
24、 根据权利要求23所述的装置,其特征在于,戶脱处理罐(101)与戶腿供应或废物罐(111, 112)之间的液,移,经由至少一个转移罐(110),其中所述转移罐(110)配有气压控制装置,包括用于将正、负气压施加到转移罐(UO)上的装置。 24. The apparatus according to claim according to claim 23, characterized in that the door off the treatment tank (101) and the fluid supply or household waste tank legs (111, 112) between the shift, via at least one transfer tank (110), wherein said transfer tank (110) with pressure control means comprises means for positive and negative pressure is applied to the transfer tank (UO) on.
25、 根据权利要求21所述的装置,其特征在于,载玻片(7)当被容纳在载玻片固定器(62)中时是可单独虫绕轴旋转的,载玻片架组件(6)配有枢轴旋车幾置,所述载玻片架组件(6)的枢轴旋織置由机器人头(22)来启动,从而根据染色规程来完成染色过程。 25. The apparatus according to claim according to claim 21, characterized in that the slides (7) are individually pivoting the worm is accommodated in a slide glass when the retainer (62), the holder assembly slides ( 6) equipped with several pivot rotation drive means, said frame assembly slides (6) pivot means by a robot spin weaving head (22) to start, thereby to complete the dyeing procedure according to the dyeing process.
Description  translated from Chinese

生辦品WW^和體 Health Office product WW ^ and body

駄领域駄 field

本发明涉及一种通过按照染色规程依次施加预定量的试剂,而对容纳在载玻片(例如载玻片)上的至少一种生物样品进行自动染色的方法和装置,其中至少一个载玻片配备在载玻片架组件中。 The present invention relates to a method and apparatus in accordance with the sequentially applied staining procedures predetermined amount of reagent, while accommodating the slides (e.g. glass slide) on at least one automated staining of biological samples, wherein the at least one glass slide frame assembly with the slides.

该申请涉及样品处理系统和生物样品处理方法领域。 The application relates to the field of biological sample processing systems and treatment samples. 本发明涉及布置在显微镜载玻片上的样品的自动加工、处理、甚至染色,并且在一些实施例中,涉及样品和载玻片的连续或批处理,以及取样系统的清洗部件。 The present invention relates to a microscope slide is arranged on the automatic processing of sample processing, even dyeing, and in some embodiments, relates to the cleaning member slides samples and continuous or batch, and the sampling system. 实施例还涉及样品处理以及样品处理的数据收集、数据维护和数据检索的控制系统。 Embodiments are also related sample handling and sample handling of data collection, data maintenance and data retrieval control system. 本发明特别涉及的应用领域包括免疫组织化学、原位杂交、荧光原位杂交、特异性染色和微阵列,以及潜在的其它化学和生物学应用。 The present invention particularly relates to the application, including immunohistochemistry, in situ hybridization, fluorescence in situ hybridization, and microarray specific staining, as well as other potential chemical and biological applications.

背景狱 Background prison

免疫组织化学(IHC)应用和其它化学及生物学分析中的样品处理,要求一个 (IHC) applications and other chemical and biological analysis of immunohistochemistry in sample handling, requires a

或许多个不同处理^jf或规程作为一个或多个样品分析的一部分。 Perhaps part of the plurality of different treatments or procedures ^ jf as one or more sample analysis. 这些样品处理次序或规程是由要求分析的个体或组织(例如医院的病理学家和组织学家)规定的, 并且进--步由待实施的特定分析指令来规定。 These samples were processed by order or regulation requires an individual or organization to analyze (such as a hospital pathologist and histologist) regulations, and into - further analysis to be implemented by a specific instruction requirements.

在样品分析的制备中,生物样品可以fflil公知的样品获取i^来取得,并且诸 In the preparation of the samples analyzed, the biological sample can fflil known sample acquiring i ^ to get, and all

如在IHC应用中通常包括组织,甚至在一些应用中可以包括一个或多个分离的细胞, 例如在微阵列样品中,所述生物样品可呈现在显微镜载玻片上。 As in IHC applications typically include tissue, and even in some applications may include one or more isolated cells, e.g., in a microarray sample, the biological sample may be presented on a microscope slide. 而且,样品可以某种保存形式不同和潜在地呈现在载玻片上。 Moreover, the sample may save some potentially presented in different forms and on a slide. 作为一个实例,样品(例如皮肤层或切片)可保存在甲醛中并呈现在载玻片上,而一个或多个石蜡或其它化学层覆盖样品。 As an example, a sample (e.g., the skin layer or slice) can be stored in formaldehyde and presented on a glass slide, and the one or more paraffin or other chemical layers covering the sample.

免疫应用例如需要包括诸如脱蜡、靶挽回(target retrieval)和染色这些步骤的处理?辨^M程,特别是对于原位杂交(ISH)技术来说。 Immune need to include applications such as dewaxing, save target (target retrieval), and the processing of these steps are stained? ^ M resolution process, particularly for in situ hybridization (ISH) technique is. 以前,在一些应用中, 这些步骤由人工来实施,因此潜在地导致这是一个耗时的规程并且需要人员主动参与到样品处理中。 Previously, in some applications, to implement these steps by hand, thus potentially leading to it is a time consuming procedure and requires people to actively participate in the sample processing. 为了满^t有利样品处理以及几乎不用人工烦琐操作的需求,已 ^ T favorable to full sample preparation and almost no manual operation cumbersome demand, has

会^f样品处理的自动化作了一些尝试。 Automation will ^ f sample made some attempts. 然而,以前的这些努力并没有充分满足对自动样品处理的需求。 However, these previous efforts did not fully meet the demand for automated sample handling. 以前对自动样品处理的努力在一些方面存在缺陷,即阻碍更稳定的自动样品处理,例如:对样品处理缺乏足够的计算机控制和监测;对处理规程和处理状态、特别fet各个样品缺乏信息共享;缺乏诊断能力;以,多个样品的批处理缺乏实时或适应能力。 Previous efforts for automatic sample handling defects exist in some respects, that impede a more stable automatic sample handling, for example: the lack of adequate sample processing computer control and monitoring; handling procedures and processing status, especially the lack of information sharing fet each sample; lack of diagnostic capacity; to batch multiple samples of a lack of real-time or ability to adapt.

过去在对呈现在载体(例如载玻片)上的样品进行自动样品处理方面的努力(例 Past efforts (for example in presenting in a carrier (such as slides) on samples of automated sample handling

如US6, 352, 861和US5, 839, 091 ),都没有提供如本文所表现的多个优点和其它特征组合。 As US6, 352, 861 and US5, 839, 091), do not provide a number of advantages as presented herein and other combinations of features.

生物样品(例如组织样品)在染色之前必须进行制备。 Biological samples (e.g., tissue sample) must be prepared prior to staining. 组织载玻片根据将要对组织实施的染色方法种类接受预处理过程。 According staining tissue slides that will be acceptable to the organization and implementation of the type of pre-treatment process. 该预处理包括脱蜡或耙挽回。 The pre-treatment includes dewaxing or rake to restore. 载玻片上 On the slide

组织的制备在其装载到自动染色仪内之前经常需要在实验室内^j:完成。 Preparation of tissue before it is loaded into the automatic staining instrument is often necessary in the laboratory ^ j: complete. 这种预处理包掛各载玻片于缓冲液或其它类型的处理液体中浸渍一预定量的时间和温度。 Such pretreatment pack slides in each buffer or linked to other types of treatment liquid immersed a predetermined amount of time and temperature.

然而,人工制备是笨重的并且预处理是不充分的,这是因为为了得到正确的预处理结果,必须非常精确地观察液体的时间和温度,这未免太苛刻了。 However, manual preparation is cumbersome and pretreatment is inadequate, it is because in order to get the right pre-treatment results, you must be very accurately observe the time and temperature of the liquid, which would be too harsh.

美国专利No: 5,839,091公开了一种自动染色装置,其中在显微镜载玻片所容纳的多个生物样品可以被处理.但是,这种仪器不l^共用于载玻片预处理的处理罐。 U.S. Patent No: 5,839,091 discloses an automatic dyeing apparatus, wherein a plurality of microscope slides of biological samples that can be processed accommodated However, this instrument is not used for a total of l ^ slides pretreated treatment tank.

发明内容 DISCLOSURE

在该背景下,本发明的一个目的是,在自动染色装置中对载玻片(例如载玻片) 上的生物样品进行自动预处理,从而生物样品的全部处理者阿以在-一个自动装置中来实施。 In this context, an object of the present invention, in the automatic staining apparatus for slides (such as slides) on the automated biological sample pretreatment, which all deal with those in the Arab-Israeli biological samples - an automatic device They are implemented.

本发明包括一种在自动染色装置中对容纳在载玻片上的至少一个生物样品进 The present invention includes a method for automatic staining apparatus housed on a slide at least one biological sample into

行处理的方法,所述方法包括以下步骤:在预定的载玻片部位配置至少一个承载生物样品的载玻片,所述载玻片配置在载玻片固定器62中基本上水平的位置上;其特征在于将所述至少一个载玻片绕枢轴旋转到基本上垂直的位置;将所述基本上垂直取向的载玻片在处理罐内浸渍一段预定的处理时间,在预定的处理时间以后从处理罐移去垂直定位的载玻片7v,并使所述的载玻片7v绕一基本7jC平的位置枢轴旋转。 Line processing method, said method comprising the steps of: configuring at least a predetermined portion of a slide glass slides bearing biological samples, the slides were arranged in the holder 62 slides in a substantially horizontal position ; wherein said at least one glass slide pivoted to a substantially vertical position; said substantially vertically oriented slide glass was immersed in the tank for a predetermined processing time processing, a predetermined processing time After removing the vertical positioning of slides 7v from the processing tank, and the slide 7v about a substantially flat position 7jC pivot. 优选的是,载玻片部位是载玻片架,其中多个载玻片配置在载玻片固定器内,而载 Preferably, the slides are slides frame portion, wherein the plurality of slides arranged in the slide glass holder, and the carrier

玻片可单独绕枢轴旋转。 Slides can be individually pivoted.

通过按照染色规程依次施加预定量的试剂,而用对容纳在载玻片上的至少一个生物样品进行自动染色的装置来实现该目的,其中至少一,玻片配置在载玻片架组件中的载玻片固定器62中的一个基本7]C平的位置,而载玻片架被设置在一个载玻片架组件中,所述的载玻片架组件包括在载玻片配置到载玻片架中之后用于预处理载玻片上的生物样品的装置,戶万述生物样品预处理装置包括配置在载玻片架组件中的处理罐;以及用于将配置在载玻片固定器中的载玻片绕枢轴旋转到垂直位置的装置,将垂直载玻片浸渍到处理罐内的^S,以及使垂直载玻片7v绕基本水平的位置枢轴旋转的装置。 By staining procedure in accordance with a predetermined amount of reagent is applied sequentially, and this object is achieved by at least one biological sample accommodated on a slide for automatic staining apparatus in which at least one slide assembly disposed on a glass slide rack carrier slide holder 62 in a substantially 7] C flat position and the slides are provided on a glass slide holder frame assembly, said slide assembly includes a frame configured to slide glass slides After the biological sample pretreatment apparatus slides for planes, ten thousand households pretreatment means comprising said biological sample is disposed in the frame assembly slides treatment tank; and means for configuring the slides in the holder slides pivoted to a vertical position means, the slides were immersed vertically to the processing tank ^ S, and the vertical slides 7v about a substantially horizontal position pivoting means.

本发明提供了一种通过将载玻片从水平位置绕枢轴旋转到垂直位置,而在自动染色装置中完成预处理的自动化方法和装置。 The present invention provides a slide glass by the pivoted from the horizontal position to the vertical position, and the completion of automated methods and apparatus for pre-processing in an automatic staining apparatus. 载玻片的这种绕枢轴旋转确保载玻片在预处理和染色过程中适当地取向。 This pivoting slides slides ensure appropriate orientation in the pretreatment and dyeing process.

通过本发明,将载玻片上生物样品的制备集成到自动染色装置中,以便使生物样品一旦容纳在载玻片上就装载到^装置内,其中预处理和染色规程«在装置内自动实施。 By the present invention, the preparation of biological samples on the slides are integrated into the automatic staining apparatus, so that the biological sample on a slide is accommodated once loaded into the ^ means, wherein the pretreatment and dyeing procedures «automatically implemented within the apparatus.

优选的是,所选择的液体从至少一个供应罐供应到一个处理罐内。 Preferably, the selected at least one liquid supply from a supply tank to the processing tank. 填充和排空此处理罐的步骤由装置的控制系统来控制,从而确保载玻片根据染色规程中有关这一点的信息接受适当的预处理步骤。 Filling and emptying steps of this treatment tank is controlled by the control system of the device, so as to ensure that the information related slides receive appropriate staining procedure according to the pre-treatment step.

优选的是,液体从供应箱转移到转移罐内并向上到达一个处理罐内。 Preferably, the liquid transfer from the supply tank to the transfer tank to reach a deal with the tank. 据此,装置«地包括气动装置,所述气动装置用于将处理液从供应罐转移到转移罐内、从转移罐转移到该处理罐内,以及将液体从该处理罐排到转移罐内、从所述转移罐排至峻物收集罐内。 Accordingly, means «comprises pneumatic means the pneumatic device for processing liquid transferred from the supply tank to the transfer tank, the transfer from the transfer tank to the processing tank, and the tank will transfer the liquid discharged from the treatment tank , Jun was collected from the tank to the transfer tank platoon. 通过用气动系统转移液体而获得的优点是,至少一个转移罐、供应罐和废物罐可位于装置外部,借此可增大容量,因为对罐的尺寸没有限制。 A pneumatic transfer system with advantages of a liquid is obtained at least one transfer tank, supply tank and waste tank may be located in an external device, whereby the capacity can be increased, because there is no restriction on the size of the tank. 而且, 通过本发明的这个实施例,可使浸渍的载玻片承受一系列的依次填充及从处理罐中排出的液体,因为系统容易适合包含几个含有不同液体的供应罐,同样还包含几个废物罐,从而可将废物分类。 Moreover, by this embodiment of the invention, can withstand a series of slides impregnated sequentially filling and discharging liquid from the treatment tank, since the system is readily adaptable comprises several supply tank containing different liquids, also several further comprising a waste tank, which can be waste.

«的是,所述的处理罐配有用于加热罐内含有的处理液的加热部件,并且加 «That said treatment tank equipped with a heating means for heating the treatment liquid contained in the tank, and add

热部件能够有利地将罐的内容物加热到至少6(TC的升高温度,更优选的是至少90 Member can advantageously heat the contents of the tank is heated to at least 6 (TC elevated temperature, more preferably at least 90

°C,甚至更优选的是至少115。 ° C, and even more preferably at least 115. C。 C. 借此,罐内的液Wjg被加热升高到12(TC,甚至有15(TC那么高,并且在该,下保持10-20分钟,而没有沸腾显示。在一个实施例中,加热部件适合将液体加热到95。C并保持40^I中,以便实施耙挽回过程。 Thereby, the liquid is heated tank Wjg raised to 12 (TC, even 15 (TC so high, and in the next 10-20 minutes, without boiling displayed. In one embodiment, the heating means is adapted The liquid is heated to 95.C and maintain 40 ^ I in order to implement rake restore process.

按照本发明的一个实施例,通过在至少一^lfe玻片绕枢轴旋转到垂直位置之后降低载玻片固定器并将载玻片浸渍到处理罐内,而将至少一个垂直载玻片浸渍到罐内。 According to one embodiment of the present invention, by ^ lfe after at least one slide pivot to a vertical position and lowering the slide glass holder slides immersed in the processing tank, and at least one vertical slide glass was immersed into the tank. 借此,得到紧凑的罐布置和载玻片固定器布局。 Thereby to obtain a compact arrangement of tanks and slides fixed layout.

组织的处理方法是组织样品的预处理。 Treatment organization is pretreated tissue samples. 然而,通过本发明,意识到,可对垂直位置的载玻片上的组织样品实施其它类型的处理,例如漂洗载玻片。 However, with the present invention, realized the tissue sample slide can vertical position on the implementation of other types of treatment, such as rinsing slides. 可以用按照本 It can be used in accordance with this

发明的装置实施的其它方ti^括组织样品的脱蜡或耙回收过程。 Other party apparatus according to the invention of ti ^ dewaxing or rake the recycling process includes a tissue sample.

按照本发明,处理罐是具有上开口槽的加长容器,戶;?M槽使垂直取向的载玻片 According to the present invention, the processing tank having an elongated open slots on the container, household;? M slides vertically aligned slots

插入罐内,以便进行处理。 Insert tank for processing. 借此,罐的体积以及由此需要的处理液的量都最小。 Thereby, the volume and the amount of the processing liquid tank are thus required minimum.

«的是,戶诚装置包括用于使排出的液体循环以便在载玻片的随后预处理过程中重新使用的装置。 «That family sincerity means includes means for discharging liquid circulation means for pre-processing in the subsequent slides re-use. 借此,用于装置操作(即组织载玻片的预处理和漂洗)的液体量可最小,从而使废物的处理容易以及成本降低。 Whereby the means for operation of the liquid volume (ie, pre-treatment and rinsing tissue slides) can be minimized, so that waste disposal easier and reduce costs.

在本发明的«实施例中,载玻片架组件配置在抽屉(drawer)组件内,其中轨道从装置中縮回,以便装载和卸载载玻片。 In the «embodiment of the present invention, the glass slide rack assembly disposed in the drawer (drawer) assembly in which the track is retracted from the apparatus for loading and unloading slides. 抽屉组件与装置的抽屉接收部件内的处理罐配合,所述处理罐能够同时处理容纳在载玻片架组件中的多个载玻片固定器内的载玻片。 Drawer treatment tank receiving member and the drawer assembly means fit within said processing tank capable of simultaneously receiving a plurality of slides in a slide glass slides were fixed to the inside of the frame assembly. 抽屉组件使装置在使用时非常灵活,如载玻片可以装载到一个抽屉中或从其上卸载下来,而其它抽屉中的载玻片可独立于其进行处理。 Drawer assembly of the device is very flexible in the use of such slides can be loaded into a drawer or unloaded therefrom, and the other drawer slides can be independent of their processing. 而且,抽屉的使用使得自动染色装置的操作容易。 Moreover, the use of drawer makes operation easy automatic staining apparatus. 每个抽屉的罐可优选地与有利地布置在装置外部的公共供应罐和废物罐相连。 Each drawer can may preferably with an external device is advantageously arranged in a common supply tank and waste tank is connected.

附图说明 Brief Description

以下参照附图来描述本发明的iM实施例,其中: Will be described below with reference to the accompanying drawings embodiments of the present invention iM, wherein:

图1是按照本发明«实施例的染色装置的示意性透视图; According to the present invention Figure 1 is «a schematic perspective view of an embodiment of the dyeing apparatus;

图2是图1所示染色装置中的工作区的顶视图; Figure 2 is a top view of the device shown in Figure 1 staining workspace;

图3是在按照本发明的装置中包括载玻片架和处理罐的抽屉组件的,性前视图; Figure 3 is a device according to the present invention comprises a frame and a slide glass treatment tank drawer assembly, the front view;

图4是闭合位置的抽屉组件的透视图; 图5是幵放位置的图4的抽屉组件; Figure 4 is a perspective view of the closed position of the drawer assembly; FIG. 5 is a discharge position 幵 drawer assembly of Figure 4;

图6是按照本发明优选实施例的载玻片架的顶部透视图; 图7是布置在抽屉组件中的载玻片架固定器和箱的细节亂图8是按照本发明«实施例的处理罐的透视图; 图9是图8的处理罐的前视图;以及 Figure 6 is a top perspective view of a slide frame according to a preferred embodiment of the present invention; FIG. 7 is disposed in a drawer slide assembly and the box frame holder 8 is a detail disorder according to the present invention, «processing embodiment perspective view of the canister; Figure 9 is a front view of the treatment tank 8; and

图10是处理罐的处理液的操纵流程图。 FIG 10 is a flowchart of a process to manipulate the treatment liquid tank.

图1表示出按照本发明的样品处理系统101的一个示意性实施例。 Figure 1 shows a sample processing system according to the present invention, 101 of exemplary embodiments. 内阁(cabinet)部分4形成系统的外部,并用于满足系统的总体结构考虑(顶部的内阁部分没有表示在图1中)。 Cabinet (cabinet) Part 4 is formed outside of the system, and to meet the overall system architecture considerations (not shown in the top part of the cabinet in Figure 1). 样品处理系统可包括多个用于操纵和处理样品及显微镜载玻片的抽屉6。 Sample processing system may include a plurality of samples and for manipulating and processing of a microscope slide drawer 6. 其它样品载玻片可与本发明相容地来容纳。 Other sample slides are compatible with the present invention to be accommodated. 每个抽屉可构造成能够容纳样品载体保留组件,例如载玻片保留组件、载体轨道、模±央或盒。 Each drawer can be configured to accommodate the sample carrier retention components such as slides retention component carrier track, mode ± center or box.

样品载玻片保留组件的一个实施例可包括载玻片保留组件6 (如图7所示)。 Example A sample slide retention assembly may include a retention assembly 6 slides (Figure 7). 载玻片保留组件可包括载玻片架、模±央或盒。 Slides retention assembly may include a slide glass frame, mode ± center or box. 载玻片保留组件6被构造成能够将多个载玻片容纳在对应于样品载玻片保留设备的至少一个结构中。 Slides retention assembly 6 is configured to be able to accommodate a plurality of slides in at least one structure corresponding to the sample slide retention device. 正如下面进一步描述的,在样品的处理中采用样品载玻片保留组件。 As described further below, using the sample slide assembly retained in the treated samples. 应i^t一步注意,样品载玻片保留组件是具有抽屉的可去除的构造,并且可堆放或驻留在其它保留组件内。 I ^ t step should be noted that sample slide retention assembly is constructed with a removable drawer, and can be stacked or reside in other retention components.

在优选实施例中,载玻片可如预处理和染色过g0f要求的那样构造在垂直及水平位置(如图3和图6所示)。 In a preferred embodiment, the slides can be pretreated and dyed as g0f constructed as required in the vertical and horizontal position (as shown in Figure 3 and Figure 6). 这允许用多种方式对载玻片进行自动预处理和染色, 包括如传统的人工实验室方法中所接受的预处理和染色。 This allows a variety of ways to automatically slides pretreatment and dyeing, including such traditional manual laboratory methods accepted by the pretreatment and dyeing. 载玻片最初装载到水平位置的载玻片保留组件(例如载玻片架)和抽屉内。 Slides initially loaded into the horizontal position slide retention components (such as a glass slide frame) and a drawer. 载玻片由可调节的载玻片支持体(如图7所示)水平支撑着。 Slides can be adjusted by the slide support level is supported (see Figure 7). 如果需要预处理(例如脱蜡),系统就将载玻片旋转到垂直位置并将这些样品下降到浸渍处理罐内(下面进一步描述),然后用所需的液体进行填充。 If desired pretreatment (e.g. dewaxing), the system will be rotated to a vertical position and slides these samples decreased to an immersion treatment tank (described further below), then filled with the desired liquid. 在一些实施例中,载玻片架降低,从而影响载玻片的下降(参见图3和图7)。 In some embodiments, the frame slides reduced, thus affecting the slide decreased (see FIG. 3 and FIG. 7). 为了在载玻片上实施染色过程,如以下所述,系统将载玻片-旋转到水平位置, 而注射器或探头将液体施加到样品上。 To implement the dyeing process on a slide, as described below, the system slides - rotated to a horizontal position, and the syringe or probe is applied to the liquid sample. 每,玻片能够单独旋转,因此可对具有不同要求的不同样品进行单独处理。 Each, slides can be rotated individually, so you can have different requirements for different samples were treated separately.

样品处理系统ia过预处理过程(例如脱蜡)可使样品的处理步骤或载玻片上呈现的细胞制备自动化。 Ia pretreated sample handling system processes (for example dewaxing) allows the preparation of automated sample processing steps or slides presented on the cell. 系统提供广泛的载玻片预处理。 The system provides a wide range of slides pretreatment. 通常实施的两种类型的预处理实例是(但不限于),脱蜡和靶挽回。 Examples of two types of pre-treatment is usually performed (but are not limited to), and the target recover dewaxing. 在一些实施例中,这些过程必须用垂直取向、 浸渍在多种液体处理罐内的载玻片来实施。 In some embodiments, these procedures must be oriented vertically, immersed in a variety of liquid treatment tank slides implemented. 脱蜡包括将载玻片—在一系列液体中浸渍 Dewaxing including slides - immersed in a series of liquid

短时间(潜在地为约5-IO辨中)。 Short-term (potentially about 5-IO identified in). 此过程预计首先从样品上去除已经固定或呈现在其中的石蜡,其次去除石蜡溶剂,然后缓慢地使样品再水合。 This process is expected to first be removed from the sample which has been fixed or presented in paraffin, followed by solvent removal of paraffin, then slowly the sample rehydrated. 靶挽回,在一些实施例中为抗原决定簇脱模,包括将载玻片浸渍在热缓冲液处理罐内,并且在一些实施例中,浸渍约20 5H中,然后将载玻片冷却约20射中。 Restore target, in some embodiments the epitope release, including the slides immersed in buffer solution heat treatment tank, and, in some embodiments, from about 20 5H impregnated, and then the slides were cooled to about 20 shot. 在优选实施例中温度维持在约95t:。 In a preferred embodiment example, the temperature is maintained at about 95t :. 在耙挽回中,标志或其它标示用来标记感兴趣的样品部分,例如其细胞或结构。 Restore the rake, the flag or other mark used to mark a sample portion of interest, e.g., its cells or structures.

为了实施这些相同的预处理过程,系统在一些实施例中模仿或对应于Al^ffi In order to implement these same pretreatment process, system, or correspond to imitate Al ^ ffi In some embodiments,

的供应源的过程和物理属性。 Process of supply and physical properties. 据此,兩己备处理罐IOI (如图3、 7和8所最佳示出的)。 Accordingly, the two have prepared the treatment tank IOI (Fig. 3, 7 and 8 best shown). 在一些实施例中,每个处理罐101的元件,如图4和5所示,构造在抽屉IOO 内。 In some embodiments, each processing element tank 101, as shown in Figure 4 and 5, constructed in a drawer IOO. 在一^M实施例中,维持实施预处理过禾Mff需的液体体积,而无需每个彼此面对面的载玻片取向(在常规系统中,它们是面对面的),尽管处理罐甚至贯穿载玻片表面来分布液体。 In one embodiment ^ M, to maintain the liquid volume pretreated embodiment Wo Mff needed, without having to face each other, each slide orientation (in conventional systems, they are face to face), even in spite of the processing tank through the slide glass the sheet surface to distribution of the liquid.

在一些实施例中,处理罐肖滩加热载玻片。 In some embodiments, the processing tank heating Shore beach slide. 此热Mil过热装置均匀i也贯穿^ 个体载玻片的表面。 This heat Mil superheater uniform surface ^ i also run through the individual slides. 热量的精确和物理施加导致处理步骤的标准化和重复性。 Heat is applied to precise and physical processing steps lead to the standardization and repeatability. 填充和加热任务由计算机控制的调度禾旨来执行(如下面进一步描述的)。 Filling and heating tasks are performed by a computer-controlled scheduling Wo aims to implement (as further described below). 考虑到ttM数目的载玻片的存在和缺失,可调节液体的体积。 Taking into account the presence and absence of ttM number of slides, you can adjust the volume of the liquid.

按照本发明的染色装置1的1M实施例在图1和2中示出。 1M dyeing device according to an embodiment of the present invention in Figures 1 and 2 show. ^fe装置1包括围绕试剂站2的矩形框架4,以及第一和第二载玻片部分5,试剂站2包括试剂瓶室, 其中每个室内放置有试剂瓶3,许多分离的轨道组件6放置在第一和第二载玻片部分5上,每个轨道组件6容纳许多一个挨一个地安装在轨道组件6内的显微镜载玻片7。 ^ Fe apparatus 1 comprises a rectangular frame 2 surrounds the reagent station 4, and first and second slide glass part 5, the reagent station 2 comprises a reagent bottle chamber, wherein each reagent bottle 3 placed indoors, many separate track assembly 6 placed on the first part and the second slide glass 5, each accommodating a plurality of track assembly 6 mounted side by side within the track assembly 6 7 microscope slides. 在图示的实施例中,每个轨道可容纳8 ^玻片,但是轨道可设计成能够容纳任何适当数目的载玻片。 In the illustrated embodiment, each track can hold 8 ^ slides, but the track may be designed to accommodate any suitable number of slides. 由于一个挨--个地布置有8个轨道,因此图示实施例可容纳高达64个载玻片7,每个在载玻片的上面都固定有样品例如组织,因此试剂可 Since one suffer - a place arranged eight tracks, so the illustrated embodiment can accommodate up to 64 slides 7, each of which is fixed in the above samples such as tissue slides, so agents can

从上方施加到每,玻片上的样品中。 From above applied to each of the sample on the slide.

用于沿X和Y (以及Z)方向(如箭头X和Y所示)移动探头10的机器人臂20, 布置在染色装置的框架4上方。 (As indicated by arrows X and Y shown) robot arm for moving the probe along the X and Y (and Z) in the direction of 10 20, is disposed above the frame 4 in the dyeing apparatus. 机器人臂20因此可以)l,头10定位在所有试剂瓶3以及所有显微镜载玻片7的上方,并且进一步操纵探头10,以吸取任一瓶3中包含的部^H式剂,从而将那部诚齐帷送并施加到任一载玻片7上,以便对每賴玻片7上的样品有选择itt行染色或处理。 Robotic arm 20 can be) l, slides the head 10 is positioned in all three reagent bottles and above all microscope 7, and further manipulate the probe 10 to absorb any portion contained in a bottle 3 ^ H-type agent, thereby it Makoto Qi curtain and applied to either send slides 7 to 7 on each sample slide Lai itt row selectively dyed or treated. 禾佣合适的控制装置,例如具有用于此目的的^it软件和输入数据的计算机(未示出),该染色装置1能够对需要不同染色或处理试齐U和过程的样品进行自动染色或处理。 Wo commission suitable control device such as a computer with ^ it software and input data used for this purpose (not shown), the dyeing device 1 is capable of staining or treating test samples of different Homogeneous U and processes need to be automated staining or deal with.

如图1 ,探头10容纳在机器人头22中并由机器人臂20来操纵。 1, the probe 10 is accommodated in the head 22 by the robot arm 20 to the robot manipulator. 探头10上升到上部位置(在Z方向),在此处清楚地看到,瓶3在探头10的下面,但是机器人包括机器人头22中用于降低探头10的装置,以便^!繊头尖浸到所选择的试剂瓶3 的内容物中并吸取选定量的用于所选择的染色或处理过程的试剂。 Probe 10 rises to the upper position (in the Z direction), clearly seen here, the probe 10 bottles 3 below, but the robot comprising a robot head 22 for lowering the probe apparatus 10 to ^! 繊 tip is immersed to the selected reagent bottle contents 3 and drawing a selected amount of reagent for the selected dyeing or processing procedure. 机器人头22还酉己有指向下方的CCD照相机25。 Unitary robot head 22 also has a CCD camera pointing downward 25. 禾,照相机确定载玻片和试剂瓶以及装置在工作区域内的其它特性的状劍言息,例如阅读配置在试剂容器上的代码,以确定系统内的试齐诉中类和试剂部位。 Wo, the camera slides and reagent bottle is determined and means other characteristics in the work area of the shaped sword made by interest rates, such as reading the configuration code on the reagent container to determine the test system QI v class and reagent site. 照相机还可确定组织样品载玻片的状态,例如特定载玻片的部位、指示有关呈现在载玻片上的组织样品或待实施的处理方案的信息的信息索弓I, 例如代码。 The camera can also determine the status of a tissue sample slides, for example, site specific slides, tissue samples related instructions presented on a glass slide or pheromone bow I plan to be implemented in the process of information, such as code.

本发明的染色装置1还包括探头清洗站8和试剂混合器9,机器人臂20还布置成能够将探头运送到清洗站8以及试剂混合器9。 A dyeing apparatus of the present invention further comprises a reagent probe wash station 8 and the mixer 9, the robot arm 20 is also arranged to be conveyed to the probe washing station 8 and a reagent mixer 9.

当载玻片架在上部位置时(如图3所示),载玻片架中的载玻片7被装载和卸载到水平位置。 When the slide glass rack in the upper position (Figure 3), slides rack slide 7 is loaded and unloaded into a horizontal position. 载玻片架布置在载玻片升降机63中,当载玻片架6在其上部位置时, 载玻片固定器62适合在水平位置和垂直位置7v之间绕枢轴转动载玻片7。 Slides slides lift frame disposed at 63, when slides frame 6 in its upper position, the holder 62 slides fit between horizontal and vertical positions 7v pivoting slides 7. 载玻片架和载玻片架升降机63被布置成抽屉组件100的移动部100a。 Rack slides and slides lifter assembly 63 is arranged in the drawer of the mobile unit 100a 100. 在抽屉组件100的相应静止部100b,配备处理罐IOI。 In the drawer assembly corresponding stationary portion 100b 100, and with the processing tank IOI.

染色装置雌地包括八个抽屉组件(如图1所示)。 Dyeing apparatus comprises eight female drawer assembly (as shown in Figure 1). 然而,应该意识到,根据设计性能,还可提l^壬何其它数目。 However, it should be appreciated that, depending on the design performance, l ^ Wang also mention what other numbers. 每个抽屉组件100包括抽屉载玻片、载玻片架升降机63、包括温度控制部件64的载玻片架组件6、处理罐IOI、用于收集染色液体的滴盘65,和包括指示器的控制装置,所述指示器用于不同使用者信息及处理监视目的。 Each drawer assembly 100 includes drawer slides, slides lifter 63, including temperature control means 64 slide rack assembly 6, the processing tank IOI, for collecting the dyeing liquid drip pan 65, and including indicators control device, the indicator for different users and process information monitoring purposes.

载玻片架61在图6中示出。 Slides frame 61 shown in FIG. 6. 载玻片架组件6包括载玻片架61,后者在各个载玻片接收室68 (如图6所示)中ttm具有8个载玻片7的容量。 6 slides frame assembly 61 includes a slide glass holder, which has the capacity of 8 slides 7 in the respective receiving chamber slides 68 (Figure 6) ttm. 与每个室68相连的是载玻片固定器62。 And each chamber 68 is connected to the holder 62 slides. 载玻片固定器62包括具有载玻片夹69和启动装置67的绕枢轴旋转装置,J5皿载波片夹69在水平载玻片位置与垂直载玻片位置之间绕枢轴旋转。 Slides holder 62 includes a pivoting device 67 slide clamp 69 and the activation means, J5 dish carrier holder 69 slides between a horizontal position and vertical position pivot slides. 载玻片7, 7v在其载玻片固定器62中单独绕枢轴旋转,而载玻片夹69通过按压所配备的两个嵌片67,可绕枢轴旋转,其中一个从水平位置旋转到垂直位置, 另一个使载玻片从垂直位置返回到水平位置。 Slides 7, 7v slide glass holder 62 in its separate pivot, and slides the clip 69 by pressing with the two panels 67, pivotally, wherein a rotation from the horizontal position to the vertical position, so that the other slides return from the vertical position to a horizontal position. 载玻片(7)当被容纳在载玻片固定器(62)中时是可单独绕枢轴旋转的,载玻片架组件(6)配有枢轴旋转装置,0M载玻片架组件(6)的枢轴旋转装置由机器人头(22)来启动,从而根据染色规程来完成染色过程。 Slides (7) When is housed in a glass slide holder (62) is pivotable about a separate slide glass frame assembly (6) with a pivoting means, 0M slides holder assembly (6) a pivoting means comprises a robot head (22) to start, thereby to complete the dyeing procedure according to the dyeing process.

当抽屉100载有一个或多个载玻片7并皿处于染色过程中时,载玻片架在上部位置。 When the drawer 100 contains one or more slides 7 and dish in the dyeing process, the glass slide rack in the upper position. 当载玻片7装载之后,载玻片7就旋转到垂直位置7v,然后载玻片架61 用载玻片升降机63陶氐,以便将垂直设置的载玻片7v浸渍到下面的处理罐101内。 When loading after 7 slides, the slides 7 is rotated to a vertical position 7v, and then slides the lift frame 61 with the slide glass 63 pottery Di to 7v slides vertically immersed into the processing tank 101 below inside. 抽屉组件也在图4, 5和7中示出。 Drawer assembly are 4, 5 and 7 are shown. 载玻片升降机63适合在载玻片7v浸渍在罐的液体中时搅动载玻片7v。 Lift 63 slides in a glass slide for slides 7v 7v dipping stir liquid in the tank when.

处理罐101填充有来自转移罐110 (参见图10)的预定量的处理液。 Processing tank 101 is filled with a predetermined amount of the treatment liquid from the transfer tank 110 (see FIG. 10). 相关的处理^]t匕前已经从供应罐111经气压^g转移到转移罐110。 Related processing ^] before t dagger has shifted from supply tank 111 through the air pressure to transfer tank 110 ^ g. 采用转移罐110以及用包括真空泵113的气动系统控制液体的转移,可完成泵给,而液体无需与泵给元件接触。 Using transfer tank 110 and includes a vacuum pump with a pneumatic control system 113 transfer liquid pump to be completed, and without the need for components in contact with the liquid pump. 这是有利的,因为液体残留在元件中的危险由此降到最小。 This is advantageous, since the risk of liquid remaining in the element thereby minimized.

雌地配备总共8个抽屉。 Female ground with a total of eight drawers. 据此,这意味着,这8个处理罐也配备在染色装置中。 Accordingly, this means that the processing tank is also equipped with eight dyeing apparatus. 每个处理罐101容纳高达8个浸渍的载玻片7v。 Each processing tank 101 accommodates up to eight slides impregnated 7v. 处理罐的主要功能是,当载玻片下降到处理罐内之后,在一定时间内(例如15併中)将罐内的液体从室温加热到预定温度,并维^f页定温度例如高达约120°C,而在预处理时间(例如10-20射中) 没有fti可沸腾显示。 The main function of the processing tank, when slides down to the treatment tank after a certain time (for example, 15 and in) the liquid tank is heated from room temperature to a predetermined temperature, and page dimension ^ f given temperature, for example up to about 120 ° C, while in the pretreatment time (for example, shot 10-20) can not fti boiling display. 雌的驢一般是约4CTC-约12CTC,正如所需的处理所确定的。 Female donkey is usually about 4CTC- about 12CTC, as determined by the desired process. 非常常见的是,iMM^在8(TC-IO(TC之间,更优选的温度在95。C-98。C之间。对于一醜殊的处理,雌的fig在110。C-13(TC之间,甚至高达约150。C。 Very common, iMM ^ between 8 (TC-IO (TC, more preferably at a temperature between 95.C-98.C. For an ugly special treatment, the female fig in 110.C-13 ( between TC, even up to about 150.C.

在处理时间之后,将热源关掉,并通过提升载玻片架61、借此将垂直载玻片'7v从处理罐101中,,来去除载玻片7v。 After processing time, turn off the heat source, and by raising a glass slide frame 61, whereby the vertical slides' 7v from the processing tank 101 ,, to remove slides 7v. 罐101可用于脱蜡、再水合以及热诱导的耙挽回。 Tank 101 can be used for dewaxing, rehydration and heat induced rake recover. 这些过程是在具有载玻片的染色装置上实施的,戶腿载玻片在垂直取向、 浸渍在填充有不同所需试剂和空的各个罐内。 These processes are having on the implementation of the dyeing device slides, indoor leg slides in a vertical orientation, immersed in and filled with different reagents required for each empty tank. 对耙挽回过程,罐内的液体水平上升到载玻片上的iH己之上。 To rake restore process, the liquid level rises above the tank iH already on the slide. 加热部件适合加热到约95",并将该温度维持高达40-60 :^中。在罐内完成的预处理过程包括将载玻片在一系列液体中浸渍短时间例如5-10 Heating means for heating to about 95 ", and the temperature is maintained as high as 40-60: ^ in the tank to complete the pretreatment process comprises the slides were immersed in a series of short-term liquid such as 5-10

射中。 Shot. 脱蜡过程预计首先从组织样品上去除固定在其上的石蜡,其次去除石蜡溶剂, 然后依次通过一系列的试剂使样品再水合。 Dewaxing process is expected to first remove paraffin fixed thereon from the tissue samples, followed by removal of paraffin solvents, followed by a series of reagent samples were rehydrated.

如诸如图8和9所示,处理罐101是加长的,具有开放槽102,可iiil槽102 插入载玻片7v。 Such as shown in FIGS. 8 and 9, the processing tank 101 is lengthened, 102, may iiil groove having an open insertion groove 102 slides 7v. 这使得罐的体积相对小些,从而又可以相当快速地加热罐内的液体和/或使加热并维繊内流1W^的能耗相当低。 This makes the volume of the tank is relatively small, which in turn can be quite rapidly heated liquid tank and / or the heating and flow in the Victoria 繊 1W ^ energy consumption is very low. 罐101经液体连接管103填充和排空,且加热部件104雌地位于罐的下部。 Tank 101 via connecting pipe 103 liquid filling and emptying, and heating the lower part of the tank 104 to the female. 罐101在两侧还配有绝缘侧壁部件,用于促进加热,由此縮短加热时间。 Tank 101 at both sides of the insulating sidewall also has means for facilitating heating, thereby shortening the heating time. 罐101还配有用于登记罐内的液体水平的传SH 装置(未示出)禾,于登记流皿度并将这些数据馈送到装置的控制系统的传感器。 SH 101 comes with tank transfer means for liquid level registered tank (not shown) Wo, in the registration of the flow pan and feeds the data to the control system of the device sensors.

预处理液或试剂可储存在许多单个容器内,其中一些容器储存用于脱蜡的液体, 一些容器用于靶挽回,而一些容器用于储存100%的乙醇、蒸馏水和缓冲液。 Pretreatment or reagents can be stored in a plurality of individual containers, wherein some of the liquid storage container for dewaxing, some containers used to restore the target, and some of the container for storing 100% ethanol, distilled water and buffers. Allow

器有利地具有与装置的预处理过程性肖^/f需的特定液体量对应的不同体积。 Advantageously has a different size and a specific amount of liquid pre-treatment process of the device Shaw ^ / f corresponding required.

供应容器m与处理罐ioi之间的液條移是经转移罐iio (如图io所示)进行的。 The supply container and the liquid treatment tank ioi m between the shift is through transfer tank iio (shown in Figure io) performed. 液体转移通过施加到转移罐iio上的正、负气压来实现。 Liquid transfer by applying to the positive and negative pressure transfer tank iio on to achieve. tt地配备两个分 tt place with two points

离的转移罐(未示出), 一个用于含水溶液,第二个用于有机溶液。 From the transfer tank (not shown), one for the aqueous solution used in the second organic solution. 同样,为了倾空 Similarly, in order emptying

处理罐,废液经转移罐110转移到废物容器112。 Treatment tank, the waste transfer tank 110 through the transfer into a waste container 112. tti^地配备废物罐,例如一个用于有毒废液, 一个用于无毒废液。 tti ^ be equipped with waste tanks, such as one for toxic waste and one for non-toxic waste.

流体可以在任何容器与任何罐之间的两个方向上转移。 Fluid can be transferred in both directions between any vessel of any tank. 流体转移的操作J吸序由 Fluid transfer order by the suction operation J

装置的控制系统来确定。 Control system devices to determine. 脱^剂可重新使用并从干净到fc脏周期性地循环。 De ^ agent can be reused from a clean to dirty fc cyclically. 用过 Used

的肮脏脱蜡液体和罐的漂洗液被使用者或控制系统当作废物弃掉。 Dewaxing dirty liquid and rinse tank is discarded as waste user or control system. 耙挽回缓冲液和 Rake restore buffers and

水在控制系统中被^H己为"一次使用(single use)",并且在使用之后转移到废物中。 Water in the control system is ^ H had to "single use (single use)", and after using a transfer to the waste.

«地,按照本发明的方法可包括:诸如在完成所需的处理之后将«片上的至少一个生物样品暂时储存在处理罐内的适当液体中,直到载玻片上的生物样品去除、用于离机处理为止。 «, The method according to the present invention may include: as required after completion of the processing will be« biological sample of at least one sheet is temporarily stored in a suitable liquid treatment tank until removal of the biological sample on a glass slide, for off- machine processed. 一般,处理罐的这种使用在过夜染色方面(例如在半夜完成)是特别有利的。 Generally, such use in the treatment tank overnight dyeing (for example, in the middle of the night to complete) is particularly advantageous.

以上按照一,选示范性实施例描述了本发明的装置和方法。 According to one or more selected from the exemplary embodiment describes an apparatus and method of the present invention. 然而,通过本发明意识到,在不脱离如所附权利要求书限定的本发明范围的前提下,可以对所述方法和装置做出许多其它变型和等同。 However, it realized by the present invention without departing from the appended claims and the scope of the invention as defined in the premise of the method and apparatus may make many other modifications and equivalents.

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
CN1350172A23 Oct 200122 May 2002莱卡显微系统努斯洛赫股份有限公司Device for treating article
US2002/0072122 Title not available
US397602817 Jan 197524 Aug 1976Newman-Howells Associates LimitedAutomatic article immersion apparatus
US408454120 Apr 197618 Apr 1978Olympus Optical Co., Ltd.Dyeing and decolorization apparatus for use in a blood serum analyzer of an electrophoretic type
US473882415 Oct 198619 Apr 1988Kabushiki Kaisha Tiyoda SeisakushoApparatus for dyeing specimens automatically preparatory to microscopic examination
US533835817 Sep 199216 Aug 1994Kabushiki Kaisha Tiyoda SeisakushoApparatus for dyeing tissues
US575803321 Jun 199626 May 1998Biotek Solutions, IncorporatedAutomated tissue assay using standardized chemicals and packages
US58390917 Oct 199617 Nov 1998Lab Vision CorporationMethod and apparatus for automatic tissue staining
US61104253 Dec 199829 Aug 2000Coulter International Corp.Blood smear slide outloader
US644417023 Mar 19993 Sep 2002Microm Laborgeräte GmbHApparatus for the treatment for specimens
WO2001/06255A1 Title not available
Legal Events
DateCodeEventDescription
25 Jan 2006C06Publication
22 Mar 2006C10Entry into substantive examination
25 Mar 2009C14Grant of patent or utility model