CN100413960C - 以少量精细胞对哺乳动物进行有性别选择的授精 - Google Patents
以少量精细胞对哺乳动物进行有性别选择的授精 Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
Description
处理 | 母牛数 | 31-41天受孕数 | 64-67天受孕数 | 雌性胎儿数 |
性别选择的精液 | 45 | 20(44%) | 19(42%) | 18(95%)<sup>a</sup> |
液体对照 | 28 | 15(54%) | 15(54%) | 8(53%)<sup>b</sup> |
冷冻对照 | 29 | 16(55%) | 15(52%) | 12(80%)<sup>c</sup> |
Claims (17)
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200810128058.9A CN101498712B (zh) | 1997-12-31 | 1998-12-31 | 以少量精细胞对哺乳动物进行有性别选择的授精 |
CN200810128060.6A CN101504406B (zh) | 1997-12-31 | 1998-12-31 | 以少量精细胞对哺乳动物进行有性别选择的授精 |
CN200810128055.5A CN101498711B (zh) | 1997-12-31 | 1998-12-31 | 以少量精细胞对哺乳动物进行有性别选择的授精 |
CN200810128059.3A CN101504405B (zh) | 1997-12-31 | 1998-12-31 | 以少量精细胞对哺乳动物进行有性别选择的授精 |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
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US09/001,394 US6149867A (en) | 1997-12-31 | 1997-12-31 | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
US09/015,454 US6071689A (en) | 1997-12-31 | 1998-01-29 | System for improving yield of sexed embryos in mammals |
US09/001,394 | 1998-01-29 | ||
US09/015,454 | 1998-01-29 |
Related Child Applications (7)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200810128059.3A Division CN101504405B (zh) | 1997-12-31 | 1998-12-31 | 以少量精细胞对哺乳动物进行有性别选择的授精 |
CN200810128060.6A Division CN101504406B (zh) | 1997-12-31 | 1998-12-31 | 以少量精细胞对哺乳动物进行有性别选择的授精 |
CN200810128056XA Division CN101496747B (zh) | 1997-12-31 | 1998-12-31 | 以少量精细胞对哺乳动物进行有性别选择的授精 |
CN2008101280625A Division CN101502450B (zh) | 1997-12-31 | 1998-12-31 | 以少量精细胞对哺乳动物进行有性别选择的授精 |
CN2008101280610A Division CN101504407B (zh) | 1997-12-31 | 1998-12-31 | 以少量精细胞对哺乳动物进行有性别选择的授精 |
CN200810128058.9A Division CN101498712B (zh) | 1997-12-31 | 1998-12-31 | 以少量精细胞对哺乳动物进行有性别选择的授精 |
CN200810128055.5A Division CN101498711B (zh) | 1997-12-31 | 1998-12-31 | 以少量精细胞对哺乳动物进行有性别选择的授精 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103555662A (zh) * | 2013-10-31 | 2014-02-05 | 大连金弘基种畜有限公司 | 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离 |
Families Citing this family (67)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU752985B2 (en) * | 1997-01-31 | 2002-10-03 | Xy, Llc. | Optical apparatus |
ATE326228T1 (de) | 1997-07-01 | 2006-06-15 | Vicam Lp | Verfahren zur geschlechtsbestimmung von säuger- nachkommenschaft |
US6071689A (en) * | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
ATE488247T1 (de) * | 1998-07-30 | 2010-12-15 | Xy Llc | Pferdesystem für die künstliche befruchtung ohne chirurgischen eingriff |
US7208265B1 (en) * | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
IT1317724B1 (it) * | 2000-01-14 | 2003-07-15 | Istituto Sperimentale Italiano | Procedimento per la produzione di embrioni non umani di sessopredeterminato ad alto valore genetico. |
AUPQ629100A0 (en) * | 2000-03-16 | 2000-04-15 | Btf Pty Ltd | Process for preparing controlled samples of particles such as microorganisms and cells |
US7371517B2 (en) | 2000-05-09 | 2008-05-13 | Xy, Inc. | High purity X-chromosome bearing and Y-chromosome bearing populations of spermatozoa |
BR0111618A (pt) * | 2000-06-12 | 2004-06-29 | Xy Inc | Sistema de controle de rebanho integrado utilizando populações isoladas de espermatozóides contendo cromosso- x e contendo coromosso y |
WO2002004006A2 (en) * | 2000-07-10 | 2002-01-17 | Pharmacia & Upjohn Company | Method for improving the efficacy of artificial insemination of non-human animals |
US20030172394A1 (en) * | 2001-08-01 | 2003-09-11 | Holzer George L | Selection and cloning methods |
US6554811B2 (en) * | 2000-09-08 | 2003-04-29 | University Of Saskatchewan Technologies Inc. | Internal artificial vagina for collecting animal semen |
AR029030A1 (es) | 2000-10-05 | 2003-06-04 | Xy Inc | Disposicion de inseminacion histeroscopica de yeguas |
US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
CA2468772C (en) | 2000-11-29 | 2013-10-29 | George E. Seidel | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
US7674576B2 (en) * | 2001-01-12 | 2010-03-09 | Abs Corporation | Semen extender composition and methods for manufacturing and using |
CA2435857A1 (en) * | 2001-01-30 | 2002-09-26 | Rebholtz, Amy K. | Methods for embryo recipient management |
US20030135876A1 (en) * | 2002-01-16 | 2003-07-17 | Larry Mobraaten | Maintenance of genetic lines using cryopreserved sperm |
CN1313061C (zh) * | 2002-03-11 | 2007-05-02 | 刘凤鸣 | 一种动物精子的性别分选方法 |
BR0312843A (pt) * | 2002-07-22 | 2005-05-10 | Xy Inc | Sistema para processar células espermáticas |
DK2275533T3 (en) * | 2002-08-01 | 2016-11-07 | Xy Llc | Sperm Cell Assessment Method |
US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
AU2003265471B2 (en) | 2002-08-15 | 2009-08-06 | Xy, Llc. | High resolution flow cytometer |
US7169548B2 (en) * | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
US20040142384A1 (en) * | 2003-01-16 | 2004-07-22 | Cohen Barb Ariel | Magnetic separator |
US7335507B2 (en) * | 2003-03-28 | 2008-02-26 | Monsanto Technology Llc | Process for the staining of sperm |
EP2309245B1 (en) | 2003-03-28 | 2015-11-25 | Inguran, LLC | Methods for providing sex-sorted animal sperm |
US20060263829A1 (en) | 2003-05-15 | 2006-11-23 | Evans Kenneth M | Efficient haploid cell sorting flow cytometer systems |
JP5046645B2 (ja) * | 2003-09-04 | 2012-10-10 | アリックス インコーポレイテッド | 血液や細胞等の液体混合物を構成成分に分離する方法、装置およびシステム。 |
US20050114915A1 (en) * | 2003-11-26 | 2005-05-26 | Vicam L.P. | Method for sex biasing spermatozoa |
EP1730523B1 (en) * | 2004-03-29 | 2010-01-13 | Inguran, LLC | Use of a composition which regulates oxidation/reduction reactions intracellularly and/or extracellularly in a staining or sorting process of spermatozoa |
CA2561519C (en) * | 2004-03-29 | 2017-08-22 | Monsanto Technology Llc | Sperm dispersions for use in insemination |
EP2269617B1 (en) * | 2004-07-22 | 2016-04-27 | Inguran, LLC | Process for enriching a population of sperm cells |
PT2884258T (pt) | 2004-07-27 | 2016-12-13 | Beckman Coulter Inc | Discriminação da citometria de fluxo melhorada com transformação geométrica implementada por computador |
US20060118167A1 (en) * | 2004-12-03 | 2006-06-08 | Xy, Inc. | Pressure regulated continuously variable volume container for fluid delivery |
US7355696B2 (en) * | 2005-02-01 | 2008-04-08 | Arryx, Inc | Method and apparatus for sorting cells |
US20060172315A1 (en) * | 2005-02-01 | 2006-08-03 | Anderson Amy L | Methods for staining cells for identification and sorting |
US7618770B2 (en) * | 2005-07-29 | 2009-11-17 | Xy, Inc. | Methods and apparatus for reducing protein content in sperm cell extenders |
AU2008350867B2 (en) | 2007-09-29 | 2014-08-28 | Timothy James Williams | Composition and method to modify sperm function and increase male gender ratio in mammals |
EP2229457A4 (en) | 2007-12-14 | 2011-01-26 | Minitube America Inc | SEX-SPECIFIC SEPARATION OF SPERM AND EMBRYOES |
JP4572973B2 (ja) | 2008-06-16 | 2010-11-04 | ソニー株式会社 | マイクロチップ及びマイクロチップにおける送流方法 |
US8251887B2 (en) * | 2009-01-24 | 2012-08-28 | Xihe Li | Reproductive technology of low dose semen production and in vitro/in vitro fertilization in domestic animals |
US8512224B2 (en) * | 2009-01-24 | 2013-08-20 | Xy, Llc | Method of producing an inseminate |
WO2011034411A2 (es) * | 2009-09-16 | 2011-03-24 | Carlos Alberto Barcelo Rojas | Método de inseminación artificial para preñar vacas |
US20110236923A1 (en) * | 2010-03-23 | 2011-09-29 | Genetics & Ivf Institute | Method for staining and sorting of a small volume of sperm |
NZ629047A (en) | 2010-06-09 | 2016-01-29 | Xy Llc | A heterogeneous inseminate system |
ITTO20100651A1 (it) | 2010-07-28 | 2012-01-29 | Ist Sperimentale It Lazzaro Spallanza | Procedimento ed apparecchiatura per la caratterizzazione e la separazione di spermatozoi con sensori micrometrici a leva sospesa |
CN103649294B (zh) * | 2011-04-29 | 2016-05-18 | 贝克顿·迪金森公司 | 多路分类系统和方法 |
US9200334B2 (en) | 2011-04-29 | 2015-12-01 | Becton, Dickinson And Company | Cell sorter system and method |
BR112014013671A2 (pt) * | 2011-12-09 | 2017-06-13 | Univ Missouri | pirofosfato inorgânico e usos do mesmo |
US9888990B2 (en) | 2012-06-06 | 2018-02-13 | Inguran, Llc | Methods for use of sex sorted semen to improve genetic management in swine |
US9433195B2 (en) | 2012-06-06 | 2016-09-06 | Inguran, Llc | Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells |
CN105557621B (zh) * | 2014-10-13 | 2018-10-16 | 中国科学院上海生命科学研究院 | 快速高效获得无菌哺乳动物的方法 |
WO2016090310A1 (en) | 2014-12-05 | 2016-06-09 | Inguran, Llc | Cell processing using magnetic particles |
WO2017062319A1 (en) | 2015-10-05 | 2017-04-13 | Becton, Dickinson And Company | Automated drop delay calculation |
ES2943382T3 (es) * | 2015-12-23 | 2023-06-12 | Siemens Healthcare Gmbh | Celda de flujo para el análisis de partículas en un líquido que va a ser examinado, procedimiento y uso |
WO2018136772A1 (en) | 2017-01-20 | 2018-07-26 | Inguran, Llc | Sperm processing method, apparatus and related media compositions |
US11376220B2 (en) | 2017-06-30 | 2022-07-05 | Therio, LLC | Single-injection methods and formulations to induce and control multiple ovarian follicles in bovine, caprine, ovine, camelid and other female animals |
WO2019043656A1 (en) | 2017-09-01 | 2019-03-07 | Genus Plc | METHODS AND SYSTEMS FOR ASSESSING AND / OR QUANTIFYING POPULATIONS OF SPERMATOZOIDS WITH SEXUAL ASYMMETRY |
US20190310243A1 (en) | 2018-04-09 | 2019-10-10 | Inguran, Llc | Methods and compositions for determining the presence or absence of dna aberrations |
JP7366931B2 (ja) | 2018-04-27 | 2023-10-23 | ベクトン・ディキンソン・アンド・カンパニー | 制御されたエアロゾル含有量を有する密閉された液滴ソータを有するフローサイトメータおよびそれを使用する方法 |
CN112136034A (zh) * | 2018-04-27 | 2020-12-25 | 贝克顿·迪金森公司 | 用于流式细胞术分选样品的收集系统及其使用方法 |
EP3942019A4 (en) | 2019-03-19 | 2022-12-14 | Inguran, LLC | METHODS OF IMPROVING SPERM POPULATIONS |
KR102316634B1 (ko) * | 2019-03-25 | 2021-10-25 | 중앙대학교 산학협력단 | 신규한 정액보존액을 이용한 암컷 수태율 증진방법 |
US11513114B2 (en) * | 2019-05-29 | 2022-11-29 | Abs Global, Inc. | Kill event optimization |
EP4168801A1 (en) | 2020-06-22 | 2023-04-26 | Westfälische Wilhelms-Universität Münster | Sperm stratification |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5135759A (en) * | 1989-05-10 | 1992-08-04 | The United States Of America As Represented By The Secretary Of Agriculture | Method to preselect the sex of offspring |
WO1996012171A2 (en) * | 1994-10-14 | 1996-04-25 | University Of Washington | High speed flow cytometer droplet formation system |
Family Cites Families (195)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US32350A (en) | 1861-05-21 | Improvement in machines for turning and spreading hay | ||
US34782A (en) * | 1862-03-25 | Improvement in lamps | ||
US3005756A (en) | 1958-11-14 | 1961-10-24 | Noland L Van Demark | Diluter containing carbon dioxide for preserving semen |
US3299354A (en) * | 1962-07-05 | 1967-01-17 | Coulter Electronics | Aperture tube structure for particle study apparatus |
US3499435A (en) * | 1967-06-02 | 1970-03-10 | Paul E Rockwell | Esophageal probe for use in monitoring |
US3547526A (en) | 1967-10-26 | 1970-12-15 | Kollsman Instr Corp | Optical beam cross-section converter |
US3810010A (en) | 1968-11-02 | 1974-05-07 | Telefunken Patent | Particle analysis method and apparatus wherein liquid containing particles is sucked into a constricted flow path |
US3829216A (en) | 1968-11-26 | 1974-08-13 | M Persidsky | Optical system and method for counting sperm cells |
DE1815352C3 (de) | 1968-12-18 | 1975-03-20 | Wolfgang Prof. Dr. Dittrich | Automatisches MeB- und Zählgerät für die Teilchen einer Dispersion |
US4327177A (en) | 1969-04-10 | 1982-04-27 | Wallace Shrimpton | Method and means for controlling the sex of mammalian offspring and product therefor |
US4474875A (en) | 1969-04-10 | 1984-10-02 | Wallace Shrimpton | Method and means for controlling the sex of mammalian offspring and product therefor |
US3894529A (en) | 1969-04-10 | 1975-07-15 | Bio Controls Inc | Method and means for controlling the sex of mammalian offspring and product therefor |
DE1919628C3 (de) | 1969-04-18 | 1975-04-10 | Wolfgang Prof. Dr. Dittrich | Anordnung zum automatischen Zählen und/oder Klassifizieren von in einem strömungsfähigen Medium dispergierten Teilchen |
US3687806A (en) | 1969-11-04 | 1972-08-29 | Bio Controls Inc | Method for controlling sex of mammalian offspring |
US3788744A (en) * | 1970-01-14 | 1974-01-29 | Bio Physics Systems Inc | Method and apparatus for photoanalysis |
US3661460A (en) | 1970-08-28 | 1972-05-09 | Technicon Instr | Method and apparatus for optical analysis of the contents of a sheathed stream |
US3687803A (en) | 1970-11-09 | 1972-08-29 | American Cyanamid Co | Acid chloride activators for hydrogen peroxide bleaching |
US3816249A (en) | 1970-11-23 | 1974-06-11 | B Bhattacharya | Universal medium and method for extending the useful life of semen in vitro |
US3644128A (en) * | 1970-12-28 | 1972-02-22 | Stuart Lipner | Method of preparing comminuted meat products |
US3756459A (en) | 1971-01-12 | 1973-09-04 | Damon Corp | Method and apparatus for metering fluid utilizing pressure differentials |
US3791384A (en) * | 1971-07-15 | 1974-02-12 | Schaumann H | Artificial insemination of sows |
US3833796A (en) | 1971-10-13 | 1974-09-03 | Georgia Tech Res Inst | Method and apparatus for chromosome digitizing |
BE793185A (fr) * | 1971-12-23 | 1973-04-16 | Atomic Energy Commission | Appareil pour analyser et trier rapidement des particules telles que des cellules biologiques |
US3826364A (en) | 1972-05-22 | 1974-07-30 | Univ Leland Stanford Junior | Particle sorting method and apparatus |
US3761941A (en) | 1972-10-13 | 1973-09-25 | Mead Corp | Phase control for a drop generating and charging system |
US3791517A (en) * | 1973-03-05 | 1974-02-12 | Bio Physics Systems Inc | Digital fluidic amplifier particle sorter |
US4009260A (en) | 1973-04-19 | 1977-02-22 | Schering Aktiengesellschaft | Fractionation of sperm |
US3893766A (en) | 1973-06-14 | 1975-07-08 | Coulter Electronics | Apparatus for orienting generally flat particles for slit-scan photometry |
US3947093A (en) * | 1973-06-28 | 1976-03-30 | Canon Kabushiki Kaisha | Optical device for producing a minute light beam |
US3944917A (en) * | 1973-08-13 | 1976-03-16 | Coulter Electronics, Inc. | Electrical sensing circuitry for particle analyzing device |
US3909744A (en) | 1973-09-24 | 1975-09-30 | United Technologies Corp | Unstable resonator system producing a high irradiance beam in the far field |
US3906929A (en) | 1973-11-23 | 1975-09-23 | Lynn Lawrence Augspurger | Processes for reproduction of cellular bodies |
FR2254639B1 (zh) | 1973-12-18 | 1978-06-02 | Agronomique Inst Nat Rech | |
US4070617A (en) * | 1974-05-08 | 1978-01-24 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Device for controlling the particle flow in an apparatus for measuring the properties of particles suspended in liquid |
US3973196A (en) | 1974-05-24 | 1976-08-03 | Coulter Electronics, Inc. | Method and apparatus for ejecting a metered amount of particulate sample |
US3963606A (en) | 1974-06-03 | 1976-06-15 | Coulter Electronics, Inc. | Semi-automatic adjusting delay for an electronic particle separator |
US3877430A (en) | 1974-07-17 | 1975-04-15 | Horst K Wieder | Artificial insemination apparatus |
US4083957A (en) | 1974-07-26 | 1978-04-11 | Lang John L | Process for the alteration of the sex-ratio of mammals |
US4006360A (en) * | 1974-08-21 | 1977-02-01 | Block Engineering, Inc. | Method of discriminating between dyed particles and background fluorescence of the dye |
USRE32350E (en) | 1974-11-22 | 1987-02-10 | Bhairab C. Bhattacharya | Thermal convection counter streaming sedimentation and forced convection galvanization method for controlling the sex of mammalian offspring |
US4092229A (en) | 1975-12-17 | 1978-05-30 | Bhattacharya Bhairab C | Thermal convection counter streaming sedimentation and forced convection galvanization method for controlling the sex of mammalian offspring |
US3976197A (en) | 1974-11-22 | 1976-08-24 | Bhattacharya Bhairab C | Thermal convection counter streaming sedimentation method and apparatus for controlling the sex of mammalian offspring |
US4014611A (en) * | 1975-04-30 | 1977-03-29 | Coulter Electronics, Inc. | Aperture module for use in particle testing apparatus |
DE2521236C3 (de) | 1975-05-10 | 1978-12-14 | Hildegard Dr. 4400 Muenster Goehde Geb. Kuhl | Einrichtung zum Zählen und Messen von in einer Flüssigkeit suspendierten Teilchen |
US3960449A (en) | 1975-06-05 | 1976-06-01 | The Board Of Trustees Of Leland Stanford Junior University | Measurement of angular dependence of scattered light in a flowing stream |
US4058732A (en) | 1975-06-30 | 1977-11-15 | Analytical Radiation Corporation | Method and apparatus for improved analytical fluorescent spectroscopy |
US4007087A (en) * | 1975-10-17 | 1977-02-08 | Gametrics Limited | Sperm fractionation and storage |
AU2154077A (en) | 1976-01-27 | 1978-07-27 | Univ Edinburgh | Control of sex ratio in mammalian offspring |
US4302166A (en) | 1976-04-22 | 1981-11-24 | Coulter Electronics, Inc. | Droplet forming apparatus for use in producing uniform particles |
US4162282A (en) | 1976-04-22 | 1979-07-24 | Coulter Electronics, Inc. | Method for producing uniform particles |
JPS5319891A (en) | 1976-06-10 | 1978-02-23 | Coulter Electronics | Method and apparatus for folling drop formation and separation |
GB1583150A (en) | 1976-08-02 | 1981-01-21 | Milk Marketing Board | Apparatus for collecting eggs |
US4110604A (en) | 1976-11-04 | 1978-08-29 | Becton, Dickinson And Company | Particle density measuring system |
DE2709399C3 (de) | 1977-03-04 | 1980-07-24 | Goehde, Wolfgang, Dr., 4400 Muenster | Einrichtung zum Messen von Zelleigenschaften |
DE2716095A1 (de) | 1977-04-12 | 1978-10-19 | Zoeld Tibor Dr Phys | Gasgesteuertes verfahren zum sortieren von in einem elektrolyten suspendierten teilchen und vorrichtung zur durchfuehrung des verfahrens |
US4448767A (en) | 1977-10-11 | 1984-05-15 | Sumar Corporation | Preparation of monospecific male-specific antibody and the use thereof for increasing the percentage of mammalian offspring of either sex |
US4191749A (en) | 1977-10-11 | 1980-03-04 | Bryant Bernard J | Method and material for increasing the percentage of mammalian offspring of either sex |
US4189236A (en) * | 1978-03-20 | 1980-02-19 | Coulter Electronics, Inc. | Ellipsoid-conic radiation collector and method |
US4179218A (en) | 1978-05-15 | 1979-12-18 | The Boeing Company | Particle size analyzer |
US4251733A (en) | 1978-06-29 | 1981-02-17 | Hirleman Jr Edwin D | Technique for simultaneous particle size and velocity measurement |
DE2832091A1 (de) | 1978-07-21 | 1980-01-31 | Eidenschink Henning | Optisches verfahren zur bestimmung der teilchengroesse kolloidaler loesungen und messgeraet zur durchfuehrung des verfahrens |
US4225405A (en) | 1978-08-16 | 1980-09-30 | Lawson Rommom L | Process for separation and collection of viable female and male spermatozoa |
US4276139A (en) | 1978-08-16 | 1981-06-30 | Lawson Rommon L | Process for magnetic separation and collection of viable female and male spermatozoa |
US4230558A (en) | 1978-10-02 | 1980-10-28 | Coulter Electronics, Inc. | Single drop separator |
US4341471A (en) | 1979-01-02 | 1982-07-27 | Coulter Electronics, Inc. | Apparatus and method for measuring the distribution of radiant energy produced in particle investigating systems |
US4267268A (en) | 1979-03-12 | 1981-05-12 | Nelson Jr Robert A | Spermatozoa extenders |
US4274408A (en) | 1979-03-26 | 1981-06-23 | Beatrice Nimrod | Method for guide-wire placement and novel syringe therefor |
US4200802A (en) | 1979-03-28 | 1980-04-29 | The United States Of America As Represented By The United States Department Of Energy | Parabolic cell analyzer |
US4408877A (en) | 1979-04-10 | 1983-10-11 | Ernst Leitz Wetzlar Gmbh | Device for hydrodynamic focussing of a particle-suspension in a liquid flow cytophotometer |
US4263508A (en) | 1979-04-20 | 1981-04-21 | Research Corporation | Pulse edge measurement for determining particle dimensional characteristics |
US4255021A (en) * | 1979-04-20 | 1981-03-10 | The United States Of America As Represented By The United States Department Of Energy | Optical device with conical input and output prism faces |
US4325483A (en) | 1979-08-20 | 1982-04-20 | Ortho Diagnostics, Inc. | Method for detecting and controlling flow rates of the droplet forming stream of an electrostatic particle sorting apparatus |
US4317520A (en) * | 1979-08-20 | 1982-03-02 | Ortho Diagnostics, Inc. | Servo system to control the spatial position of droplet formation of a fluid jet in a cell sorting apparatus |
US4318481A (en) * | 1979-08-20 | 1982-03-09 | Ortho Diagnostics, Inc. | Method for automatically setting the correct phase of the charge pulses in an electrostatic flow sorter |
US4318480A (en) | 1979-08-20 | 1982-03-09 | Ortho Diagnostics, Inc. | Method and apparatus for positioning the point of droplet formation in the jetting fluid of an electrostatic sorting device |
US4318482A (en) * | 1979-08-20 | 1982-03-09 | Ortho Diagnostics, Inc. | Method for measuring the velocity of a perturbed jetting fluid in an electrostatic particle sorting system |
DE2943116C2 (de) | 1979-10-25 | 1986-06-19 | Gesellschaft für Strahlen- und Umweltforschung mbH, 8000 München | Einrichtung zur durchflußcytometrischen Reaktions- und/oder Diffusionsmessung |
US4400764A (en) | 1980-02-05 | 1983-08-23 | The Boeing Company | Low backscatter illumination system |
US4367043A (en) * | 1980-05-05 | 1983-01-04 | Leland Stanford Junior University | Method and means for delivering liquid samples to a sample scanning device |
US4362246A (en) | 1980-07-14 | 1982-12-07 | Adair Edwin Lloyd | Method of treating collected mammal semen and separating sperm into X Y components |
US4348107A (en) | 1980-07-18 | 1982-09-07 | Coulter Electronics, Inc. | Orifice inside optical element |
US4350410A (en) | 1980-10-08 | 1982-09-21 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Multiprism collimator |
US4487320A (en) | 1980-11-03 | 1984-12-11 | Coulter Corporation | Method of and apparatus for detecting change in the breakoff point in a droplet generation system |
US4395676A (en) | 1980-11-24 | 1983-07-26 | Coulter Electronics, Inc. | Focused aperture module |
US4361400A (en) | 1980-11-26 | 1982-11-30 | The United States Of America As Represented By The United States Department Of Energy | Fluidic assembly for an ultra-high-speed chromosome flow sorter |
US4680258A (en) | 1981-03-24 | 1987-07-14 | Sloan-Kettering Institute For Cancer Research | Process for sex determination in man by use of monoclonal antibodies to the H-Y antigen |
US4339434A (en) | 1981-08-17 | 1982-07-13 | Gametrics Limited | Method of increasing the incidence of female offspring |
US4395397A (en) | 1981-09-17 | 1983-07-26 | Sidney Farber Cancer Institute, Inc. | Apparatus and method for killing unwanted cells |
US4422761A (en) | 1981-09-28 | 1983-12-27 | Frommer Joseph C | Photo-electric particle sensing system |
US4515274A (en) | 1981-12-02 | 1985-05-07 | Coulter Corporation | Particle analyzing and sorting apparatus |
US4511661A (en) | 1982-03-19 | 1985-04-16 | University Patents, Inc. | ATCC HB8116 And its monoclonal anti-H-Y antibody, Hyclonalan |
US4498766A (en) * | 1982-03-25 | 1985-02-12 | Becton, Dickinson And Company | Light beam focal spot elongation in flow cytometry devices |
US4559309A (en) | 1982-09-01 | 1985-12-17 | Memorial Sloan Kettering Cancer Center | Flow cytometry-fluorescence measurements for characterizing sperm |
US4501366A (en) * | 1982-12-14 | 1985-02-26 | Adolph Coors Company | Photomultiplier tube assembly |
US4492436A (en) * | 1983-01-03 | 1985-01-08 | At&T Bell Laboratories | Polarization independent beam splitter |
JPS59143146A (ja) * | 1983-02-07 | 1984-08-16 | Nippon Kogaku Kk <Nikon> | ミラ−集光型照明光学系 |
IT1197570B (it) * | 1983-02-11 | 1988-12-06 | Serono Ist Farm | Miscele di fsh e lh da ipofisi porcine in rapporto definito |
US4523809A (en) | 1983-08-04 | 1985-06-18 | The United States Of America As Represented By The Secretary Of The Air Force | Method and apparatus for generating a structured light beam array |
US4538733A (en) | 1983-10-14 | 1985-09-03 | Becton, Dickinson And Company | Particle sorter with neutralized collection wells and method of using same |
US4585736A (en) | 1983-10-18 | 1986-04-29 | The United States Of America As Represented By The United States Department Of Energy | Flow cytometric measurement of total DNA and incorporated halodeoxyuridine |
US4573796A (en) | 1984-01-06 | 1986-03-04 | The United States Of America As Represented By The United States Department Of Energy | Apparatus for eliminating background interference in fluorescence measurements |
US4545677A (en) | 1984-03-05 | 1985-10-08 | Becton, Dickinson And Company | Prismatic beam expander for light beam shaping in a flow cytometry apparatus |
US4600302A (en) | 1984-03-26 | 1986-07-15 | Becton, Dickinson And Company | Flow cytometry apparatus with uniform incoherent light excitation |
US4605558A (en) | 1984-04-20 | 1986-08-12 | Wallace Shrimpton | Process for cell separation |
US4660971A (en) | 1984-05-03 | 1987-04-28 | Becton, Dickinson And Company | Optical features of flow cytometry apparatus |
FR2563726B1 (fr) * | 1984-05-04 | 1986-10-10 | Robert Cassou | Appareil d'insemination artificielle, notamment des carnivores |
EP0171676B1 (en) | 1984-07-31 | 1990-11-07 | Hitachi, Ltd. | Free-flow electrophoretic separation method and apparatus therefor |
US4598408A (en) | 1984-10-22 | 1986-07-01 | Trw Inc. | High extraction efficiency cylindrical ring resonator |
US4989977A (en) * | 1985-07-29 | 1991-02-05 | Becton, Dickinson And Company | Flow cytometry apparatus with improved light beam adjustment |
US4999283A (en) | 1986-01-10 | 1991-03-12 | University Of Kentucky Research Foundation | Method for x and y spermatozoa separation |
US5162306A (en) * | 1987-01-23 | 1992-11-10 | Donaldson Lloyd E | Composition and method for producing superovulation in mammals |
US4780451B1 (en) * | 1987-01-23 | 1995-04-04 | Asua International Inc | Composition and method for producing superovulation in cattle |
EP0286088B1 (en) * | 1987-04-08 | 1994-09-14 | Hitachi, Ltd. | A sheath flow type flow-cell device |
US5346990A (en) | 1987-04-08 | 1994-09-13 | Cytogam, Inc. | Sex-associated membrane proteins and methods for increasing the probability that offspring will be of a desired sex |
US5021244A (en) | 1988-12-06 | 1991-06-04 | Cytogam, Inc. | Sex-associated membrane antibodies and their use for increasing the probability that offspring will be of a desired sex |
FR2614626B1 (fr) * | 1987-04-30 | 1989-07-21 | Ranoux Claude | Conteneur pour fecondation des ovocytes et replacement des embryons chez l'homme et l'animal |
US4987539A (en) * | 1987-08-05 | 1991-01-22 | Stanford University | Apparatus and method for multidimensional characterization of objects in real time |
US5712807A (en) * | 1987-10-21 | 1998-01-27 | Bangham; James Andrew | Pulse analyzing method and apparatus |
GB8726304D0 (en) * | 1987-11-10 | 1987-12-16 | Secr Defence | Particle asymmetry analyser |
US4988619A (en) | 1987-11-30 | 1991-01-29 | United States Department Of Energy | Flow cytometry apparatus |
JPH0718785B2 (ja) * | 1988-09-19 | 1995-03-06 | 株式会社日立製作所 | フローセル装置 |
US5726009A (en) * | 1989-03-20 | 1998-03-10 | Anticancer, Inc. | Native-state method and system for determining viability and proliferative capacity of tissues in vitro |
US4981580A (en) * | 1989-05-01 | 1991-01-01 | Coulter Corporation | Coincidence arbitration in a flow cytomery sorting system |
DE68924275T2 (de) * | 1989-05-12 | 1996-05-02 | Cytogam Inc | Mit dem geschlecht assoziierte membranproteine und methoden zur erhöhung der wahrscheinlichkeit, dass die nachkommenschaft das gewünschte geschlecht besitzt. |
JPH0353164A (ja) * | 1989-07-20 | 1991-03-07 | Canon Inc | サンプル供給装置及びこれを用いたサンプル測定装置 |
US5098657A (en) * | 1989-08-07 | 1992-03-24 | Tsi Incorporated | Apparatus for measuring impurity concentrations in a liquid |
JP2808321B2 (ja) * | 1989-09-19 | 1998-10-08 | 東亜医用電子株式会社 | 細胞分析方法及び装置 |
US5275787A (en) * | 1989-10-04 | 1994-01-04 | Canon Kabushiki Kaisha | Apparatus for separating or measuring particles to be examined in a sample fluid |
EP0422616B1 (en) * | 1989-10-11 | 1996-02-07 | Canon Kabushiki Kaisha | Apparatus for and method of fractionating particle in particle-suspended liquid in conformity with the properties thereof |
EP0448931B1 (en) * | 1990-01-26 | 1996-04-03 | Canon Kabushiki Kaisha | Method for measuring a specimen by the use of fluorescence light |
US5150313A (en) | 1990-04-12 | 1992-09-22 | Regents Of The University Of California | Parallel pulse processing and data acquisition for high speed, low error flow cytometry |
US5087295A (en) * | 1990-06-13 | 1992-02-11 | Becton Dickinson And Company | Cleaning cycle for flow cytometers |
DE9107792U1 (zh) * | 1991-06-25 | 1991-09-12 | Labotect-Labor-Technik, Goettingen, Gmbh, 3406 Bovenden, De | |
US5488469A (en) * | 1991-08-30 | 1996-01-30 | Omron Corporation | Cell analyzing apparatus |
US5866344A (en) * | 1991-11-15 | 1999-02-02 | Board Of Regents, The University Of Texas System | Antibody selection methods using cell surface expressed libraries |
US5400179A (en) * | 1992-02-18 | 1995-03-21 | Asahi Kogaku Kogyo Kabushiki Kaisha | Optical multilayer thin film and beam splitter |
US5298967A (en) * | 1992-06-02 | 1994-03-29 | Pacific Scientific Company | Measurement of concentrations of dissolved solvent |
US5466572A (en) | 1992-09-03 | 1995-11-14 | Systemix, Inc. | High speed flow cytometric separation of viable cells |
US5395588A (en) * | 1992-12-14 | 1995-03-07 | Becton Dickinson And Company | Control of flow cytometer having vacuum fluidics |
JP3052665B2 (ja) | 1993-01-26 | 2000-06-19 | 株式会社日立製作所 | フローセル装置 |
US5494795A (en) * | 1993-05-05 | 1996-02-27 | The United States Of America As Represented By The Secretary Of The Navy | Specific oligonucleotide primers for detection of pathogenic campylobacter bacteria by polymerase chain reaction |
CA2140926A1 (en) * | 1993-06-04 | 1994-12-05 | Byung H. Chung | Artificial insemination and embryo transfer device |
US5483469A (en) | 1993-08-02 | 1996-01-09 | The Regents Of The University Of California | Multiple sort flow cytometer |
US5596401A (en) * | 1993-09-16 | 1997-01-21 | Toa Medical Electronics Co., Ltd. | Particle analyzing apparatus using a coherence lowering device |
US5480774A (en) * | 1993-10-14 | 1996-01-02 | A/F Protein, Inc. | Determination of genomic sex in salmonids |
GB9324938D0 (en) * | 1993-12-04 | 1994-01-26 | Atomic Energy Authority Uk | Aerosol generator |
DE4419894A1 (de) * | 1994-06-07 | 1995-12-14 | Gip Medizin Technik Gmbh | Endoskopische Punktionsnadelvorrichtung |
US5601234A (en) * | 1994-08-01 | 1997-02-11 | Abbott Laboratories | Fluid nozzle and method of introducing a fluid |
US5700692A (en) | 1994-09-27 | 1997-12-23 | Becton Dickinson And Company | Flow sorter with video-regulated droplet spacing |
US5602349A (en) | 1994-10-14 | 1997-02-11 | The University Of Washington | Sample introduction system for a flow cytometer |
US5643796A (en) | 1994-10-14 | 1997-07-01 | University Of Washington | System for sensing droplet formation time delay in a flow cytometer |
US5602039A (en) | 1994-10-14 | 1997-02-11 | The University Of Washington | Flow cytometer jet monitor system |
JP3411112B2 (ja) * | 1994-11-04 | 2003-05-26 | シスメックス株式会社 | 粒子画像分析装置 |
JP3347495B2 (ja) * | 1994-11-14 | 2002-11-20 | シスメックス株式会社 | 粒子分析装置 |
US5495719A (en) * | 1994-11-14 | 1996-03-05 | Gray, Jr.; Carl O. | Method of preserving spermatozoa |
US5514537A (en) | 1994-11-28 | 1996-05-07 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Process and apparatus for sorting spermatozoa |
US5632754A (en) * | 1994-12-23 | 1997-05-27 | Devices For Vascular Intervention | Universal catheter with interchangeable work element |
FI98765C (fi) * | 1995-01-16 | 1997-08-11 | Erkki Soini | Virtaussytometrinen menetelmä ja laite |
US5608519A (en) * | 1995-03-20 | 1997-03-04 | Gourley; Paul L. | Laser apparatus and method for microscopic and spectroscopic analysis and processing of biological cells |
FR2734637B1 (fr) * | 1995-05-24 | 1997-08-14 | Abx Sa | Dispositif d'inspection optique d'un fluide, notamment pour analyses hematologiques |
US5589457A (en) * | 1995-07-03 | 1996-12-31 | Ausa International, Inc. | Process for the synchronization of ovulation |
US5726751A (en) * | 1995-09-27 | 1998-03-10 | University Of Washington | Silicon microchannel optical flow cytometer |
JP3584108B2 (ja) * | 1996-01-08 | 2004-11-04 | キヤノン株式会社 | レンズ鏡筒 |
US5707808A (en) * | 1996-04-15 | 1998-01-13 | The Regents Of The University Of California | Optical selection and collection of DNA fragments |
JP2963393B2 (ja) * | 1996-06-14 | 1999-10-18 | 浜松ホトニクス株式会社 | 光電子増倍管用電圧分割回路 |
US5872627A (en) * | 1996-07-30 | 1999-02-16 | Bayer Corporation | Method and apparatus for detecting scattered light in an analytical instrument |
US5719667A (en) * | 1996-07-30 | 1998-02-17 | Bayer Corporation | Apparatus for filtering a laser beam in an analytical instrument |
US5873254A (en) * | 1996-09-06 | 1999-02-23 | Interface Multigrad Technology | Device and methods for multigradient directional cooling and warming of biological samples |
AU752985B2 (en) | 1997-01-31 | 2002-10-03 | Xy, Llc. | Optical apparatus |
US5874266A (en) * | 1997-03-27 | 1999-02-23 | Palsson; Bernhard O. | Targeted system for removing tumor cells from cell populations |
US5985216A (en) * | 1997-07-24 | 1999-11-16 | The United States Of America, As Represented By The Secretary Of Agriculture | Flow cytometry nozzle for high efficiency cell sorting |
US5876942A (en) * | 1997-07-24 | 1999-03-02 | National Science Council Of Republic Of China | Process for sexing cow embryos |
US6149867A (en) * | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
US6071689A (en) * | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
EP1058688A4 (en) | 1998-02-03 | 2004-10-06 | Xy Inc | USE OF SPECIFIC OLIGONUCLEOTIDE PRIMERS TO DETECT THE PRESENCE OF MALE BOVINE CHROMOSOME BY CHAIN AMPLIFICATION BY POLYMERASE |
CA2321075C (en) | 1998-02-20 | 2009-10-06 | Cytomation, Inc. | A vibratory system for a sorting flow cytometer |
US6175409B1 (en) * | 1999-04-02 | 2001-01-16 | Symyx Technologies, Inc. | Flow-injection analysis and variable-flow light-scattering methods and apparatus for characterizing polymers |
ATE488247T1 (de) | 1998-07-30 | 2010-12-15 | Xy Llc | Pferdesystem für die künstliche befruchtung ohne chirurgischen eingriff |
US6150119A (en) * | 1999-01-19 | 2000-11-21 | Caliper Technologies Corp. | Optimized high-throughput analytical system |
US6193647B1 (en) * | 1999-04-08 | 2001-02-27 | The Board Of Trustees Of The University Of Illinois | Microfluidic embryo and/or oocyte handling device and method |
JP5087192B2 (ja) * | 1999-11-30 | 2012-11-28 | インテレクソン コーポレイション | 細胞群内の特定細胞に選択的に照準付けする方法及び装置 |
US6503698B1 (en) * | 2000-06-16 | 2003-01-07 | The United States Of America As Represented By The Secretary Of Agriculture | Cryopreservation of swine embryos |
US20040005582A1 (en) * | 2000-08-10 | 2004-01-08 | Nanobiodynamics, Incorporated | Biospecific desorption microflow systems and methods for studying biospecific interactions and their modulators |
US20040031071A1 (en) * | 2000-10-05 | 2004-02-12 | Xy, Inc. | System of hysteroscopic insemination of mares |
AU2002220392A1 (en) * | 2000-11-09 | 2002-05-21 | Universiy of Guelph | Mammalian sex selection using genetic modification |
US7713687B2 (en) * | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
US6849423B2 (en) * | 2000-11-29 | 2005-02-01 | Picoliter Inc | Focused acoustics for detection and sorting of fluid volumes |
JP2002208277A (ja) * | 2001-01-05 | 2002-07-26 | Toshiba Corp | 半導体記憶装置のセンスアンプ制御回路 |
US6673095B2 (en) * | 2001-02-12 | 2004-01-06 | Wound Healing Of Oklahoma, Inc. | Apparatus and method for delivery of laser light |
EP1377811B1 (en) * | 2001-04-03 | 2008-07-16 | Micronics, Inc. | Split focusing cytometer |
US6849394B2 (en) * | 2002-02-21 | 2005-02-01 | Minitube Of America | Compositions comprising reproductive cell media and methods for using such compositions |
US7335507B2 (en) * | 2003-03-28 | 2008-02-26 | Monsanto Technology Llc | Process for the staining of sperm |
US20050011582A1 (en) * | 2003-06-06 | 2005-01-20 | Haug Jeffrey S. | Fluid delivery system for a flow cytometer |
US7591064B2 (en) * | 2005-07-20 | 2009-09-22 | Hitachi Global Storage Technologies Netherlands B.V. | Method of fabrication for tape medium read head with unitary formation of multiple elements |
US7618770B2 (en) * | 2005-07-29 | 2009-11-17 | Xy, Inc. | Methods and apparatus for reducing protein content in sperm cell extenders |
-
1998
- 1998-01-29 US US09/015,454 patent/US6071689A/en not_active Expired - Lifetime
- 1998-12-31 DE DE19882943T patent/DE19882943T1/de not_active Ceased
- 1998-12-31 PT PT98965046T patent/PT1044262E/pt unknown
- 1998-12-31 NZ NZ522607A patent/NZ522607A/en not_active IP Right Cessation
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- 1998-12-31 CN CNB988132559A patent/CN100413960C/zh not_active Expired - Lifetime
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- 1998-12-31 WO PCT/US1998/027909 patent/WO1999033956A1/en active IP Right Grant
- 1998-12-31 EP EP98965046A patent/EP1044262B1/en not_active Revoked
- 1998-12-31 GB GB0016132A patent/GB2350619B/en not_active Expired - Lifetime
- 1998-12-31 CA CA2316080A patent/CA2316080C/en not_active Expired - Lifetime
- 1998-12-31 AT AT98965046T patent/ATE411379T1/de active
- 1998-12-31 DK DK07008703.6T patent/DK1818391T3/en active
-
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- 1999-11-24 US US09/448,643 patent/US6372422B1/en not_active Expired - Lifetime
-
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- 2000-06-30 NO NO20003424A patent/NO20003424L/no not_active Application Discontinuation
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- 2002-02-20 US US10/081,955 patent/US7629113B2/en not_active Expired - Fee Related
- 2002-02-20 JP JP2002044035A patent/JP4577666B2/ja not_active Expired - Lifetime
-
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- 2005-12-28 JP JP2005380486A patent/JP2006115856A/ja not_active Withdrawn
-
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- 2007-06-22 JP JP2007165690A patent/JP2007325596A/ja not_active Withdrawn
-
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- 2010-02-02 HK HK10101116.5A patent/HK1134006A1/xx not_active IP Right Cessation
- 2010-02-02 HK HK10101120.9A patent/HK1134007A1/xx not_active IP Right Cessation
- 2010-06-18 JP JP2010139978A patent/JP2010252801A/ja not_active Withdrawn
-
2013
- 2013-02-06 JP JP2013021330A patent/JP2013078346A/ja not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5135759A (en) * | 1989-05-10 | 1992-08-04 | The United States Of America As Represented By The Secretary Of Agriculture | Method to preselect the sex of offspring |
WO1996012171A2 (en) * | 1994-10-14 | 1996-04-25 | University Of Washington | High speed flow cytometer droplet formation system |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103555662A (zh) * | 2013-10-31 | 2014-02-05 | 大连金弘基种畜有限公司 | 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离 |
CN103555662B (zh) * | 2013-10-31 | 2015-09-16 | 大连金弘基种畜有限公司 | 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离 |
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