CN100389321C - Liquid level stabilizing device in micro-analysis chip liquid pool and method of use thereof - Google Patents
Liquid level stabilizing device in micro-analysis chip liquid pool and method of use thereof Download PDFInfo
- Publication number
- CN100389321C CN100389321C CNB031154859A CN03115485A CN100389321C CN 100389321 C CN100389321 C CN 100389321C CN B031154859 A CNB031154859 A CN B031154859A CN 03115485 A CN03115485 A CN 03115485A CN 100389321 C CN100389321 C CN 100389321C
- Authority
- CN
- China
- Prior art keywords
- liquid
- liquid storage
- storage tank
- level
- chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
The present invention relates to an integrated liquid level stable device for a liquid storage pool on a micro analysis chip, and a use method thereof. The device is characterized in that the liquid storage pool on the micro analysis chip is composed of a connecting passage (4) and a horizontal inlet with a liquid level stable function or an outlet passage (5). The present invention has the advantages that when liquid flow enters or flow out the liquid storage pool in the process of analysis within the time range of effective control, the present invention can maintain liquid level constant in the liquid storage pool on the micro analysis chip so as to avoid generating interference to the chip analysis process because of liquid level changes in the liquid storage pool.
Description
Technical field
The field that the present invention relates to is the micro-fluidic chip analysis, particularly relates to level stability device and using method thereof in the microanalysis chip liquid storage tank.
Background technology
Usually, microanalysis chip is made of two-layer or multilayer chiop up and down, and the material of chip is monocrystalline silicon or quartzy or glass or high molecular polymer etc.Area of chip is about several square centimeters, and the microchannel width and the degree of depth are micron order, is generally closed microchannel network, by liquid storage tank turnover sample and the reagent that links to each other with the microchannel perpendicular to chip.
This patent relates to is liquid storage tank system on the relevant microanalysis chip.Liquid storage tank on the current chip, document (Manz A, Fettinger J C, Verpoorte E, Ludi H, Widmer H M, Harrison D J, Trend in Anal.Chem., 1991,10:144) (Harrison D J, Manz A, Fan Z, Ludi H, Widmer H M, Anal.Chem., 1992,64:1926) (Jacobson S C, Hergenroder R, Koutny L B, Warmack R J, Ramsey J M, Anal.Chem., 1994,66:1107) the single radial cut type liquid storage tanks that adopt more, promptly the liquid storage tank bottom communicates with microchannel on the chip, upper opening carries out the import and export of liquid flow transmission for extraneous with liquid storage tank vertically upward.Liquid forms in its import or exit passageway and between the ambient atmosphere that the two-phase contact interface---liquid level, liquid level height and position in vertical direction is called liquid level in the liquid storage tank.Interphase interface in the liquid storage tank between liquid and ambient atmosphere presents the configuration of horizontal liquid level.Liquid flows to out liquid storage tank can produce liquid level---and the variation of liquid level changes the operate as normal that can disturb microanalysis chip when serious.Liquid storage tank employing conduit on the class chip is in communication with the outside and carries out liquid flow transmission (Ausserer W A, Bousse L, Gallagher S J, Kennedy C B in addition, Phan H L, JALA, 2001,6,69) (Lin Y H, Lee G B, Li C W, Huang G R, Chen S H, J.Chromatogr.A, 2001,937,115), but usually the position and the configuration of its outlet or inlet ducts do not carried out level control, thereby only have circulation and transfer function, do not have the level stability function.(Fang Q such as Fang Qun, Xu G M, Fang Z L, Anal.Chem.2002,74:1223) a kind of overflow-type liquid storage tank level stability method of report, its principle is when having liquid to flow to into liquid storage tank, adopts to make unnecessary liquid by liquid storage tank method of overflowing suitable for reading, keeps the level stability in the liquid stream introducing process.This method has been applicable to when liquid flows to into liquid storage tank, keeps the level stability in the liquid storage tank; Then inapplicable for the simple liquid storage tank that has liquid stream to flow out.
Summary of the invention
The object of the invention provides a kind of integrated level stability device and using method thereof that is used for liquid storage tank on the microanalysis chip.Guarantee in analytic process, as liquid flow to into or when flowing out this liquid storage tank, it is invariable that the liquid level of liquid storage tank keeps within a certain period of time, to avoid because the interference that the liquid storage tank liquid level change is produced to the chip analysis process.
The invention provides the integrated level stability device that is used for liquid storage tank on the microanalysis chip, utilize liquid stream in the horizontal channel, to flow and can not produce the characteristics of the variation of liquid level, realize the stable of the interior liquid level of liquid storage tank, all or part of employing of the import and export passage horizontal channel configuration of liquid storage tank on the chip.
According to the level stability device in the microanalysis chip liquid storage tank of the present invention, described microanalysis chip is by last, following two compositions, last or following sheet processing microchannel, punching forms the interface channel of microchannel and outside liquid on chip last slice or following sheet, around interface channel mouth level of processing passage, liquid storage tank is made of interface channel and horizontal channel, liquid communicates with the microchannel in the liquid storage tank, liquid storage tank has import or the exit passageway that communicates with ambient atmosphere, form two-phase contact interface---liquid level in the liquid storage tank between liquid and the ambient atmosphere, as liquid flow to into or when flowing out liquid storage tank, translation on the liquid level occurred level direction, and do not have displacement on the vertical direction, realize level stability.
According to the present invention advantageously, this level stability device is integrated is machined on the microanalysis chip.
According to the present invention, the horizontal channel configuration is meant that the axis of this passage is positioned on the same surface level vertical with the action of gravity direction, and simultaneously, the vertical cross-section configuration of passage and area thereof all keep constant.
According to the using method of apparatus of the present invention, utilize the liquid self character, the surface nature of tube wall reaches the interaction between liquid, tube wall, the atmosphere three, forms the stable arc liquid level of erectting in the horizontal channel.Different interactions, the configuration difference of its arc liquid level, the key of this method are to form the stable arc liquid level of erectting in the horizontal channel.As liquid flow to into or when flowing out liquid storage tank, the translation on this liquid level occurred level direction, and do not have displacement on the vertical direction.Thus, the inflow and the outflow of liquid stream, the variation that all can not produce liquid level in the liquid storage tank.
According to the using method of apparatus of the present invention, adopt with on the microanalysis chip all the horizontal channel of liquid storage tanks be positioned over method on the same surface level, can realize the level stability of total system on the same microanalysis chip.The level stability of total system is most important to stablizing of chip capillary cataphoresis system works on the chip.
Using method according to apparatus of the present invention is characterized in that, this method is relevant to liquid flowing speed in effective acting time of level stability in the liquid storage tank and the liquid storage tank, the internal diameter (cross-sectional area) of horizontal channel, length; Under certain liquid flowing speed, the cross-sectional area of the passage of improving the standard, length, the time that helps improving the liquid storage tank level stability; The raising liquid body of the internal diameter of horizontal channel, atmosphere, the restriction of the interaction force size between the tube wall three.If the internal diameter of horizontal channel is excessive, then be difficult to form therein stable setting liquid level,
Major advantage of the present invention is: in analytic process, in the time range of effectively control, can keep the constant of the interior liquid level of microanalysis chip liquid storage tank.If liquid capacity is bigger in the liquid storage tank horizontal channel, and liquid flowing speed is lower, then can reach very long stabilization time, as a few hours even a couple of days.
Another advantage of the present invention is for the liquid storage tank that has liquid stream to flow into, the liquid storage tank of liquid stream outflow is arranged perhaps, and perhaps existing liquid stream flows into the liquid storage tank that outflow is arranged again, all can adopt this device and using method to realize level stability, and application surface is wider.Simultaneously, this device integrability is machined on the microanalysis chip.
Another advantage of the present invention is, all adopts this device and using method as whole liquid storage tanks on the microanalysis chip, then can realize the stable of the interior hydraulic pressure of whole microanalysis chip channel network system.This advantage is particularly important to the chip capillary cataphoresis system.
Another advantage of the present invention is, can be applicable to flow type and varies in the liquid storage tank, realizes the level stability in the liquid storage tank in the chip continuous sample introducing process.
The present invention can be widely used in the microanalysis chip analysis field.
Description of drawings
Fig. 1. be side sectional view according to embodiments of the invention 1.
Fig. 2. be side sectional view according to the embodiment of the invention 2.
Fig. 3. be side sectional view according to the total system level stability microanalysis chip of the embodiment of the invention 3.
Fig. 4. be the side sectional view that is equipped with according to the segregation drive microanalysis chip of the embodiment of the invention 4 devices.
Fig. 5. be side sectional view according to the embodiment of the invention 5.
Fig. 6. be side sectional view according to the embodiment of the invention 6.
Fig. 7. be the structural drawing that is equipped with according to the segregation drive microanalysis chip that is used for hydrogen peroxide assay of the preferred embodiment of the present invention 7 devices.
Fig. 8. demonstration is equipped with the outcome record figure of the microchip analysis system of Fig. 7 embodiment 7 devices to the detection of variable concentrations hydrogen peroxide
Fig. 9. be to be equipped with to be used for segregation drive multiphase layer flow point that serum potassium analyzes structural drawing from microanalysis chip according to the preferred embodiment of the present invention 8 device.
Figure 10. demonstration is equipped with the outcome record figure of the microchip analysis system of Fig. 9 embodiment 8 devices to the detection of variable concentrations potassium.
Embodiment
With reference to accompanying drawing, below will describe in detail according to a preferred embodiment of the invention.
Fig. 1. be 1 side sectional view according to a preferred embodiment of the present invention.Microanalysis chip by last (1), down (2) two form, adopt bonding method to realize going up (1), (2) two involution down, in last (1) processing microchannel (3).Go up punching last (1), form interface channel (4).Chip is erect and is placed, and directly forms liquid storage tank on last (1) surface around interface channel (4) mouthful fixing horizontal passage (a 5) pipe.Liquid (6) communicates the phase interface that liquid (6) contacts with ambient atmosphere in the liquid storage tank with microchannel (3)---and it is curved to erect liquid level (7).
Fig. 2. be the side sectional view of another preferred embodiment 2 according to the present invention.Microanalysis control chip by last (1), down (2) two form.Adopt bonding method to realize last (1), descend (2) two involution.In last (1) processing microchannel (3).Go up punching last (1), form interface channel (4).Chip level is placed, and directly forms liquid storage tank on last (1) surface around interface channel (4) mouthful fixing horizontal passage (a 5) pipe.Liquid (6) communicates the phase interface that liquid (6) contacts with ambient atmosphere in the liquid storage tank with microchannel (3)---and it is curved to erect liquid level (7).
Fig. 3. be the side sectional view of total system level stability microanalysis chip according to the preferred embodiment of the invention.Microanalysis chip by last (1), down (2) two form.Adopt bonding method to realize last (1), descend (2) two involution.In last (1) processing microchannel (3).Each makes a call to a hole to go up two ends, microchannel (3) last (1), forms interface channel (4).Chip level is placed, and directly on last (1) surface, forms liquid storage tank around interface channel (4) mouthful fixing horizontal passage (a 5) pipe.Liquid (6) communicates the phase interface that liquid (6) contacts with ambient atmosphere in the liquid storage tank with microchannel (3)---and it is curved to erect liquid level (7).Two horizontal channels are on the same surface level.
Fig. 4. be the side sectional view that is equipped with according to the segregation drive microanalysis chip of preferred embodiment of the present invention device.Microanalysis chip by last (1), down (2) two form.Adopt bonding method to realize last (1), descend (2) two involution.In last (1) processing microchannel (3).Go up two ends, microchannel (3) last (1) and make a call to a hole, form interface channel (4).Chip is vertically placed, and forms liquid storage tank around interface channel (a 4) mouthful fixing horizontal tube channel.Liquid (6) communicates the phase interface that liquid (6) contacts with ambient atmosphere in the liquid storage tank with microchannel (3)---and it is curved to erect liquid level (7).Two horizontal channels (5) are on the different level, so that fluid-operated power to be provided.
Fig. 5. be the side sectional view of another preferred embodiment according to the present invention.This embodiment is used to keep the flow type sample to introduce the stable of the interior liquid level of liquid storage tank.In (1), upper end, the microchannel (3) between (2) two punching down on the microanalysis chip, form the interface channel (4) that connects chip.Chip is vertically placed.Import and outlet difference fixing horizontal passage (5) pipe in interface channel (4) both sides.When sample or other liquid stream (6) are introduced in this passage, can keep the stable of liquid level (7) liquid level.
Fig. 6. be the side sectional view of another preferred embodiment according to the present invention.This embodiment is that another kind is used to keep the flow type sample to introduce the stable device of liquid level in the liquid storage tank.In (1), the end punching of the microchannel (3) between (2) two down on the microanalysis chip, form interface channel (4).Chip is vertically placed.Around interface channel (4) mouthful fixing horizontal tube channel (5).Level is inserted sample and is introduced conduit (8) in horizontal channel (5) pipe.Sample or other liquid stream (6) can keep the stable of liquid level (7) liquid level when conduit (8) is introduced in the passage liquid storage tank.
Fig. 7. be the structural drawing that is equipped with according to the segregation drive microanalysis chip that is used for hydrogen peroxide assay of preferred embodiment of the present invention device.Microanalysis chip (9) is a glass material.The wide 50-100 micron in microchannel (3), dark 30 microns.Microchannel import and endpiece are processed aperture (10), (11), (12), (15) of the about 2mm of diameter respectively on chip.Chip is vertically placed.Center on hole (10), (11), (12), (15) fixing horizontal tube channel formation liquid storage tank.Add the chemical illuminating reagent luminol solution in the liquid storage tank that hole (10) is communicated with; Add the catalyzer potassium ferricyanide solution in the liquid storage tank that hole (11) is communicated with; Add the hydrogen peroxide test solution in the liquid storage tank that hole (12) is communicated with.The liquid storage tank that hole (15) is communicated with is the outlet pond that waste liquid flows out.The liquid stream that converges of three liquid stream carries out chemiluminescence detection at detection zone (13).Chip (16) utilizes the liquid level difference between horizontal liquid storage tank (10), (11), (12) and (15) that the liquid power that stream flows is provided, and keeps the stable of the interior liquid level of each liquid storage tank when measuring.
Fig. 8. demonstration is equipped with the outcome record figure of the microchip analysis system of Fig. 7 embodiment 7 devices to the detection of variable concentrations hydrogen peroxide.Alternating sequence is introduced superoxol (a) 2mM H
2O
2, (b) 1mM H
2O
2, blank solution (c).
Fig. 9. be to be equipped with to be used for segregation drive multiphase layer flow point that serum potassium analyzes structural drawing from microanalysis chip according to the preferred embodiment of the present invention 8 device.Microanalysis chip (9) is bonded by glass sheet and the silicone rubber plate that is shaped on passage.Chip is wide 2 centimetres, and is high 3 centimetres.Long 2 centimetres of main channel (23), wide 250 microns, dark 25 microns.Sample intake passage and sample output passage are all wide 150 microns, dark 25 microns.Terminal or the middle about 2 millimeters aperture of diameter (17), (18), (21), (22), (16) of processing respectively of sample introduction and sample output passage on chip.Chip is vertically placed.Center on hole (17), (18), (21), (16) fixing horizontal tube channel formation liquid storage tank.Hole (17) is acceptable solution (19)---the import of 0.9% sodium chloride solution, hole (21) they are acceptable solution (19) outlet.Hole (18) is test solution (20)---the import of potassium standard solution or blood sample, and hole (16) are the outlet of test solution (20).The miniature potassium ion-selective electrode of processing in hole (22).Chip (9) utilizes the liquid level difference between horizontal liquid storage tank that the liquid power that stream flows is provided, and keeps the stable of the interior liquid level of each liquid storage tank when measuring.
Figure 10. demonstration is equipped with the outcome record figure of the microchip analysis system of Fig. 9 embodiment 8 devices to the detection of variable concentrations potassium.(its concentration is 10 successively for the record spectrogram of potassium ion standard solution and typical curve
-4M, 10
-3M, 10
-2M, 10
-1M).
Claims (3)
1. the level stability device in the microanalysis chip liquid storage tank, described microanalysis chip is by last (1), following (2) two compositions, in last (1) or following sheet (2) processing microchannel (3), in last slice (1) of microanalysis chip or down sheet (2) is gone up punching, form the interface channel (4) of microchannel (3) and extraneous liquid, horizontal channel (5) around interface channel (4) mouthful Working liquids, constitute liquid storage tank by interface channel (4) and horizontal channel (5), liquid (6) communicates with microchannel (3) in the liquid storage tank, liquid storage tank has import or the exit passageway that communicates with ambient atmosphere, it is characterized in that, liquid (6) the arc liquid level (7) of formation setting that horizontal channel (5) in, contacts in the liquid storage tank with ambient atmosphere, as liquid flow to into or when flowing out liquid storage tank, translation on liquid level (7) the occurred level direction, and do not have displacement on the vertical direction, realize level stability.
2. the level stability device in the microanalysis chip liquid storage tank according to claim 1 is characterized in that this device is integrated to be machined on the microanalysis chip.
3. the level stability device in the microanalysis chip liquid storage tank according to claim 1, it is characterized in that, employing with on the microanalysis chip all the horizontal channel of liquid storage tanks place method on the same surface level, realize the level stability of total system on the same microanalysis chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031154859A CN100389321C (en) | 2003-02-19 | 2003-02-19 | Liquid level stabilizing device in micro-analysis chip liquid pool and method of use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031154859A CN100389321C (en) | 2003-02-19 | 2003-02-19 | Liquid level stabilizing device in micro-analysis chip liquid pool and method of use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1523345A CN1523345A (en) | 2004-08-25 |
| CN100389321C true CN100389321C (en) | 2008-05-21 |
Family
ID=34284307
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031154859A Expired - Fee Related CN100389321C (en) | 2003-02-19 | 2003-02-19 | Liquid level stabilizing device in micro-analysis chip liquid pool and method of use thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100389321C (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1052301C (en) * | 1994-08-01 | 2000-05-10 | 罗克贺德马丁能源系统有限公司 | Apparatus and method for performing microfluidic manipulations for chemical analysis and synthesis |
| US6120666A (en) * | 1996-09-26 | 2000-09-19 | Ut-Battelle, Llc | Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same |
| WO2001071331A1 (en) * | 2000-03-17 | 2001-09-27 | Spectrumedix Corporation | Electrophoresis microchip and system |
| WO2001079529A1 (en) * | 2000-04-17 | 2001-10-25 | Purdue Research Foundation | Biosensor and related method |
| WO2002063288A1 (en) * | 2001-01-31 | 2002-08-15 | Sau Lan Tang Staats | Microfluidic devices |
-
2003
- 2003-02-19 CN CNB031154859A patent/CN100389321C/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1052301C (en) * | 1994-08-01 | 2000-05-10 | 罗克贺德马丁能源系统有限公司 | Apparatus and method for performing microfluidic manipulations for chemical analysis and synthesis |
| US6120666A (en) * | 1996-09-26 | 2000-09-19 | Ut-Battelle, Llc | Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same |
| WO2001071331A1 (en) * | 2000-03-17 | 2001-09-27 | Spectrumedix Corporation | Electrophoresis microchip and system |
| WO2001079529A1 (en) * | 2000-04-17 | 2001-10-25 | Purdue Research Foundation | Biosensor and related method |
| WO2002063288A1 (en) * | 2001-01-31 | 2002-08-15 | Sau Lan Tang Staats | Microfluidic devices |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1523345A (en) | 2004-08-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Erbacher et al. | Towards integrated continuous-flow chemical reactors | |
| US20020150502A1 (en) | Surface tension reduction channel | |
| US20040043506A1 (en) | Cascaded hydrodynamic focusing in microfluidic channels | |
| US11911763B2 (en) | Microfluidic device and methods | |
| ES2794634T3 (en) | Procedure to generate drops | |
| CA2402591A1 (en) | Methods of reducing fluid carryover in microfluidic devices | |
| JP2007506080A (en) | Flow monitoring microfluidic device | |
| US20070077169A1 (en) | Microchip and liquid mixing method and blood testing method using this microchip | |
| CN1668527A (en) | Actuator in a microfluidic system for inducing electroosmotic liquid movement in a micro channel | |
| Johann et al. | A simple mechanism for reliable particle sorting in a microdevice with combined electroosmotic and pressure‐driven flow | |
| CA2758739C (en) | A gas-free fluid chamber | |
| CN100592084C (en) | Micro chemical chip | |
| CN100389321C (en) | Liquid level stabilizing device in micro-analysis chip liquid pool and method of use thereof | |
| CN101175992B (en) | electrochemical cell and reference cell with flowing liquid junction | |
| JP4836272B2 (en) | Liquid introduction device | |
| US7186382B2 (en) | Microchip apparatus | |
| JP2004150980A (en) | Liquid sending device and liquid feed method of microchemical/biochemical chip | |
| CN1172183C (en) | Liquid introduction device for microanalysis chip | |
| CN212586240U (en) | Simple micro-fluidic detection device | |
| JP2005147840A (en) | Plastic substrate for microflow device | |
| CN110465337B (en) | Chip and liquid flow control method and application thereof | |
| CN219682560U (en) | Microfluidic bubble removing device | |
| JP4555610B2 (en) | Gas-liquid reaction unit and analyzer | |
| JP2005329364A (en) | Gas-liquid reaction unit and analyzer | |
| CN203355750U (en) | Intelligent micro-scale liquid flow control and compensation technique system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080521 Termination date: 20110219 |