CA2698716A1 - Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods - Google Patents
Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods Download PDFInfo
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- CA2698716A1 CA2698716A1 CA2698716A CA2698716A CA2698716A1 CA 2698716 A1 CA2698716 A1 CA 2698716A1 CA 2698716 A CA2698716 A CA 2698716A CA 2698716 A CA2698716 A CA 2698716A CA 2698716 A1 CA2698716 A1 CA 2698716A1
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- leu
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
Abstract
The present invention provides novel purified and isolated nucleotide sequences encoding the cGMP-binding, cGMP-specific phosphodiesterase designated cGB-PDE. Also provided by the invention are methods and materials for the recombinant production of cGB-PDE polypeptide products and methods for identifying compounds which modulate the enzymatic activity of cGB-PDE polypeptides. <IMAGE>
Description
SEQUENCE LISTING
(1) GENERAL INFORMATION:
(i) APPLICANT: The Board of Regents of the University of Washinqton (ii) TITLE OF INVENTION: Cyclic GMP-Binding, Cyclic GMP-Specific Phosphodiesterase Materials and Methods (iii) NUMBER OF SEQUENCES: 23 (iv) CORRESPONDENCE ADDRESS:
(A) ADDRESSEE: Marshall, O=Toole, Gerstein, Murray &
Borun (B) STREET: 6300 Sears Tower, 233 S. Wacker Drive (C) CITY: Chicago (D) STATE: Illinois (E) COUNTRY: USA
(F) ZIP: 60606 (v) COMPUTER READABLE FORM:
(A) MEDIUM TYPE: Floppy diek (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS
(D) SOFTWARE: PatentIn Release #1.0, Version #1.25 (vi) CURRENT APPLICATION DATA:
(A) APPLICATION NUMBER:
(B) FILING DATE:
(C) CLASSIFICATION:
(vii) PRIOR APPLICATION DATA:
(A) APPLICATION NUMBER: US 08/068,051 (B) FILING DATE: 27-MAY-1993 (viii) ATTORNEY/AGENT INFORMATION:
(A) NAME: Noland, Greta E.
(8) REGISTRATION NUMBER: 35,302 (C) REFERENCE/DOCRET NUMBBR: 32083 (ix) TELECOMMUNICATION INFORMATION:
(A) TELEPHONE: (312) 474-6300 (8) TELEFAX: (312) 474-0448 (C) TELEX: 25-3856 (2) INFORMATION FOR SEQ ID NO:l:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 20 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:
Arg Glu Xaa Asp Ala Asn Arg Ile Asn Tyr Met Tyr Ala Gln Tyr Val Lys Aen Thr Met (2) INFORMATION FOR SEQ ID NO:2:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 9 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:
Gln 5er Leu Ala Ala Ala Val Val Pro (2) INFORMATION FOR SEQ ID NO:3:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 8 amino acids (B) TYPE: amino acid (D) TOPOLOGYs linear (ii) MOLECULE TYPEs peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:
Phe Asp Asn Asp Glu Gly Glu Gin (2) INFORMATION FOR SEQ ID NO:4:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: D'NA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:
(2) INFORMATION FOR SEQ ID NO:5:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(iv) ANTI-SENSE: YES
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:
(2) INFORlSATION FOR SEQ ID NO:6 (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: aingle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:6 (2) INFORMATION FOR SEQ ID P0:7 (i) SEQUENCE CHARACTERISTICSs (A) LENGTH: 23 base pairs (B) TYPE,: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(iv) ANTI-SENSE: YES
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:7 (2) INFORMATION FOR SEQ ID NOs8 (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 36 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(iv) ANTI-SENSE: YES
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:8 (2) INFORMATION FOR SEQ ID N0:9 (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 4474 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: CDNA
(ix) E'E:ATURE:
(A) NAME/KEY: CDS
(B) LOCATION: 99..2723 (xi) SEQUENCE DESCRIPTION: SEQ ID N0:9 Met Glu Arg Ala Gly Pro Gly Cys Arg Ala Ala Ala Thr Ala Met Gly Pro Gly Leu Gly Arg Ser Val Ala Gly Arg Ser Leu Gly Leu Tyr Leu Leu Tyr Phe Val Arg Lys Gly Thr Arg Glu Met Val Asn Ala Trp Phe Ala Glu Arg Val His Thr Ile Pro Val Cys Lys Glu Gly Ile Lys Gly His Thr Glu Ser Cys Ser Cys Pro Leu Gin Pro Ser Pro Arg Ala Glu Ser Ser Val Pro Gly Thr Pro Thr Arg Lys Ile Ser Ala Ser Glu Phe Asp Arg Pro Leu Arg Pro Ile Val Ile Lys Asp Ser Glu Gly Thr Val Ser Phe Leu Ser Asp Ser Asp Lys Lys Glu Gln Met Pro Leu Thr Ser Pro Arg Phe Asp Asn = Asp Glu Gly Asp Gln Cys Ser Arg Leu Leu Glu Leu Val Lys Asp Ile Ser Ser His Leu Asp Val Thr Ala Leu Cys His Lys Ile Phe Leu His Ile His Gly Leu Ile Ser Ala Asp Arg Tyr'Ser Leu Phe Leu Val Cys Glu Asp Ser Ser Asn Asp Lys Phe Leu Ile Ser Arg Leu Phe Asp Val Ala Glu Gly Ser Thr Leu Glu Glu Ala Ser Asn Asn Cys Ile rlrg Leu Glu Trp Asn Lys Gly Ile Val Gly His Val Ala Ala ?he Gly Glu Pro Leu Asn Ile Lys Asp Ala Tyr Glu Asp Pro Arg Phe Asn Ala Glu Val Asp Gln Ile Thr Gly Tyr Lys Thr Gln Ser Ile Leu Cys Met Pro Ile 250 255 _ 260 Lys Asn His Arg Glu Glu Val Val Gly Val Ala Gln Ala Ile Asn Lys Lys Ser Gly Asn Gly Gly Thr Phe Thr Glu Lys Asp Glu Lys Asp Phe Ala Ala Tyr Leu Ala Phe Cys Gly I1e Val Leu His Asn Ala Gln Leu Tyr Glu Thr Ser Leu Leu Giu Asn Lys Arg Asn Gln Val Leu Leu Asp CTT GCT AGC TTA ATT TTT GAA GAA CAA CAA TCA TTA c,aA GTA ATT CTA 1121 Leu Ala Ser Leu Ile Phe Glu Glu Gln Gln Ser Leu Glu Val Ile Leu Arg Lys Ile Ala Ala Thr Ile Ile Ser Pro Met Gin Val Gln Lys Cys Thr Ile Phe Ile V,al Asp G1u Asp Cys Ser Asp Ser Phe Ser Ser Val Phe His Met Glu Cys Glu Glu Leu Glu Lys Ser Ser Asp Thr Leu Thr Arg Glu Arg Asp Ala Thr Arg Ile Asn Tyr Met Tyr Ala Gln Tyr Val 390 395 400 ' 405 Lys Asn Thr Met Glu Pro Leu Asn ILa Pro Asp Val Ser Lys Asp Lys Arg Phe Pro Trp Thr Asn Glu Asn Met Gly Asn Ile Asn Gln Gln Cys Ile Arq Ser Leu Leu Cys Thr Pro Ile Lys Asn Gly Lys Lys Asn Lys Val Ile Gly Val Cys Gln Leu Val Asn Lys Met Glu Glu Thr Thr Gly Lys Val Lys Ala Phe Aen Arg Aen Aep Glu Gln Phe Leu Glu Ala Phe Val Iie Phe Cys Gly Leu Gly Ile Gln Aen Thr Gin Met Tyr Glu Ala Val Glu Arg Ala Het Ala Lys Gln Met Val Thr Leu Glu Val Leu Ser Tyr His Ala Ser Ala Ala Glu Glu Glu Thr Arg Glu Leu Gln Ser Lou Ala Ala Ala Val Val Pro Ser Ala Gln Thr Leu Lye Ile Thr Asp Phe Ser Phe Ser Asp Phe Glu Leu Ser Asp Leu Glu Thr Ala Leu Cys Thr Ile Arg Met Phe Thr Asp Leu Asn Leu Val Gln Asn Phe Gln Met Lys His Glu Val Leu Cys Lye Trp Ile Leu Ser Val Lys Lys Aen Tyr Arg Lye Aen Val Ala Tyr His Aen Trp Arg His Ala Phe Asn Thr Ala Gln Cys Met Phe Ala Ala Leu Lys Ala Gly Lys Ile Gln Lys Arg Leu Thr Asp Leu Glu Ile Leu Ala Leu Leu Ile Ala Ala Leu Ser His Asp Leu Asp His Arg Gly Val Asn Asn Ser Tyr ile Gln Arg Ser Glu His Pro Leu Ala Gin Leu Tyr Cye His Ser Ile Met Glu His His His Phe Asp Gln Cys Leu Met Ile Leu Aen Ser Pro Gly Aen Gln Ile Leu Ser Gly Leu Ser Ile Glu Glu Tyr Lye Thr Thr Leu Lys Ile Ile Lye Gln Ala Ile Leu Ala Thr Asp Leu Ala Leu Tyr Ile Lys Arg Arg Gly Glu Phe Phe Glu Leu Ile Met Lye Aen Gln Phe Aen Leu Glu Asp Pro His Gln Lys Glu Leu Phe Leu Ala Met Leu Met Thr Ala Cys Asp Leu Ser Ala Ile Thr Lys Pro Trp Pro Ile Gln Gln Arg Ile Ala Glu Leu Val Ala Thr Glu Phe Phe Asp Gln Gly Asp Arg Glu Arg Lys Giu-Leu Asn Ile Glu Pro Ala Asp Leu Met Asn Arg Glu Lys Lys Asn Lys Ile Pro Ser Met Gln Val Gly Phe Ile Asp Ala Ile Cys Leu Gln Leu Tyr Glu Ala Leu Thr His Val Ser Glu Asp Cys Phe Pro Leu Leu Asp Gly Cys Arg Lys Asn Arg Gln Lys Trp Gln Ala Leu Ala Glu Gln Gln Glu Lys Thr CTG ATC AAT GGT GeIA AGC AGC CAG ACC AAC CGA CAG CAA CGG AAT TCC 2705 Leu Ile Asn Gly Glu Ser Ser Gin Thr Asn Arg Gln Gln Arg Asn Ser Val Ala Val Gly Thr Val GAATTCCCTT TAPrAATGTGT CAGACAGTAG CTTCTTGGGC CGGGTTGTTC CCGCAGCTCC 3173 (2) INFORMATION FOR SEQ ID NO:10:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 87S amino acids (8) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTIONs SEQ ID N0:10:
Met Glu Arg Ala Gly Pro Gly Cys Arg Ala Ala Ala Thr Ala Met Gly Pro Gly Leu Gly Arg Ser Val Ala Gly Arq Ser Leu Gly Leu Tyr Leu Leu Tyr Phe Val Arg Lys Gly Thr Arg Glu Met Val Asn Ala Trp Phe Ala Glu Arg Val His Thr Ile Pro Val Cys Lys Glu Gly Ile Lys Gly His Thr Glu Ser Cys Ser Cys Pro Leu Gln Pro Ser Pro Arg Ala Glu Ser Ser Val Pro Gly Thr Pro Thr Arg Lys Ile Ser Ala Ser Glu Phe Asp Arg Pro Leu Arg Pro Ile Val Ile Lys Asp Ser Glu Gly Thr Val Ser Phe Leu Ser Asp Ser Asp Lys Lys Glu Gln Met Pro Leu Thr Ser Pro Arg Phe Asp Asn Asp Glu Gly Asp Gln Cys Ser Arg Leu Leu Glu Leu Val Lys Asp Ile Ser Ser His Leu Asp Val Thr Ala Leu Cys His Lys Ile Phe Leu His Ile His Gly Leu Ile Ser Ala Asp Arg Tyr,Ser Lou Phe Leu Val Cys Glu Asp Ser Ser Asn Asp Lys Phe Leu Ile Ser Arg Leu Phe Asp Val Ala Glu Gly Ser Thr Leu Glu Glu Ala Ser Asn Asn Cys Ile Arg Leu Glu Trp Asn Lys Gly Ile Val Gly His Val Ala Ala Phe Gly Glu Pro Leu Asn Ile Lys Asp Ala Tyr Glu Asp Pro Arg Phe Asn Ala Glu Val Asp Gln Ile Thr Gly Tyr Lys Thr Gln Ser Ile Lou Cys Met Pro Ile Lys Asn His Arg Glu Glu Va1 Val Gly Val Ala Gln Ala Ile Asn Lys Lys Ser Gly Asn Gly Gly Thr Phe Thr Glu Lys Asp Glu Lys Asp Phe Ala Ala Tyr Lou Ala Phe Cys Gly Zle Val Lou His Asn Ala Gln Leu Tyr Glu Thr Ser Leu Lou Glu Asn Lys Arg Asn Gin Val Leu Lou Asp Lou Ala Ser Lou Ile Phe Clu Glu Gln Gln Ser Lou Glu Val Ile Leu Arg Lys Ile Ala Ala Thr Ile Ile Ser Pro Met Gln Val Gln Lys Cys Thr Ile Phe Ile Val Asp Glu Asp Cys Ser Asp Ser Phe Ser Ser Val Phe His Met Glu Cy^ Glu Glu Leu Glu Lys Ser Ser Asp Thr Leu Thr Arg Glu Arg Asp Ala Thr Arg Ile Asn Tyr Met Tyr Ala Gln Tyr Val Lys Asn Thr Met Glu Pro Lou Asn Ile Pro Asp Val Ser Lys Asp Lye Arg Phe Pro Trp Thr Asn Glu Asn Met Gly Asn Ile Asn Gln Gln Cys Ile Arg Ser Leu Leu Cys Thr Pro Ile Lys Asn Gly Lys Lys Asn Lys Val Ile Gly Val Cys Gin Leu Val Asn Lys Met Glu Glu Thr Thr Gly Lys Val Lys Ala Phe Asn Arg Asn Asp Glu Gln Phe Leu Glu Ala Phe Val Ile Phe Cys Gly Leu Gly Ile Gln Asn Thr Gln Met Tyr Glu Ala Val Glu Arg Ala Met Ala Lys Gln Met Val Thr Leu Glu Val Leu Ser Tyr His Ala Ser Ala Ala Glu Glu Glu Thr Arg Glu Leu Gln Ser Leu Ala Ala Ala Val Val Pro Ser Ala Gln Thr Leu Lys Ile Thr Asp Phe Ser Phe Ser Asp Phe Glu Lou Ser Asp Lou Glu Thr Ala Lou Cys Thr Ile Arg Met Phe Thr Asp Lou Asn Leu Val Gln Ann Phe Gln Met Lys His Glu Val Leu Cys Lys Trp Ile Leu Ser Val Lys Lys Asn Tyr Arg Lys Asn Val Ala Tyr His Asn Trp Arg His Ala Phe Ann Thr Ala Gln Cys Met Phe Ala Ala Lou Lys Ala Gly Lys Ile Gln Lye Arg Lou Thr Asp Leu Glu Ile Lou Ala Leu Lou Ile Ala Ala Leu Ser His Asp Leu Asp His Arg Gly Val Asn Asn Ser Tyr Ile Gln Arg Ser Glu His Pro Lou Ala Gln Leu Tyr Cys His Ser Ile Met Glu His His His Phe Asp Gin Cys Lou Met Ile Leu Asn Ser Pro Gly Asn Gln Ile Lou Ser Gly Lou Ser Ile Glu Glu Tyr Lys Thr Thr Lou Lys Ile Ile Lys Gln Ala Ile Leu Ala Thr Asp Leu Ala Leu Tyr Ile Lys Arg Arg Gly Glu Phe Phe Glu Leu Ile Met Lys Asn Gln Phe Asn Leu Glu Asp Pro His Gln Lys Glu Leu Phe Lou Ala Met Lou Met Thr Ala Cys Asp Lou Ser Ala Ile Thr Lys Pro Trp Pro Ile Gln Gin Arg Ile Ala Glu Leu Val Ala Thr Glu Phe Phe Asp Gln Gly Asp Arg Glu Arg Lys Glu Leu Asn Ile Glu Pro Ala Asp Lou Met Asn Arg Glu Lys Lys Asn Lys Ile Pro Ser Met Gln Val Gly Phe Ile Asp Ala Ile Cys Leu Gln Leu Tyr Glu Ala Leu Thr His Val Ser Glu Asp Cys Phe Pro Leu Leu Asp Gly Cys Arg Lys Aen Arg Gln Lys Trp Gln Ala Leu Ala Glu Gln Gln Glu Lys Thr Leu Ile Asn Gly Glu Ser Ser Gln Thr Asn Arg -5a Gln Gln Arg Asn Ser Val Ala Val Gly Thr Val (2) INFORMATION FOR SEQ ID NO:11:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 2060 baee paire (8) TYPE: nucleic acid (C) STRANDEDNESS: sinqle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:lls (2) INFORMATION FOR SEQ ID N0:12:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 1982 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) HOLECULE TYPE: cDNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:
GGTTCAATGC AGAAGTTGAC CAAATTACAG GCTACAAGAC AC7lAAGCATT CTTTGTATGC 300 AGTTTCTGGA AGCTTTTGTC ATCTTTTGTG GCTTGGGGAT'CCAGAACACG CAGATCTATG 1020 TGAAACATGA GGTTCTTTGC AGATGGATTT TAAGTGTTAA GAAGAATTAT CGGAAGAATG 1320*
(2) INFORMATION FOR SEQ ID N0:13:
(i) SEQUENCS CHARACTERISTICS:
(A) LENGTH: 18 base pairs (8) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:
(2) INFORMATION FOR SEQ ID NO:14:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 base pairs (B) TYPEs nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ( ii ) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:14:
(2) INFORMATION FOR SEQ ID NO:15:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:
(2) INFORMATION FOR SEQ ID N0:16:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPEt DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:16:
(2) INFORMATION FOR SEQ ID NO:17:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 1107 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: eDNA
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:17:
(2) INFORMATION FOR SEQ ID NO:18:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NOs18:
(2) INFORMATION FOR SEQ ID NO:19:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:19:
(2) INFORMATION FOR SEQ ID N0:20:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 30 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:20:
(2) INFORMATION FOR SEQ ID NO:21:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NOt21:
(2) INFORMATION FOR SEQ ID NO:22:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 2645 base pairs (8) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA
(ix) FEATURE:
(A) NAME/1CEY: CDS
(B) LOCATION: 12..2636 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22:
Met Glu Arg Ala Gly Pro Ser Phe Cly Gln Gln Arg Gln Gln Gin Gin Pro Gln Gln Gln Lys Gin Gln Gln Arg Asp Gln Asp Ser Val Glu Ala Trp Leu Asp Asp His Trp Asp Phe Thr Phe Ser Tyr Phe Val Arg Lys Ala Thr Arg Glu Met Val Asn Ala Trp PheAla Glu Arg Val His Thr Ile Pro Val Cys Lys Glu Gly Ile Arg Giy His Thr Glu Ser Cys Ser Cys Pro Leu Gln Gln Ser Pro Arg Ala Asp Asn Ser Val Pro Gly Thr Pro Thr Arg Lys Ile Ser Ala Ser Glu Phe Asp Arg Pro Leu Arg Pro Ile Val Val Lys Asp Ser Glu Gly Thr Val Ser Phe Leu 110 ~15 '20 - ~~-2 5 Ser Asp Ser Glu Lys Lye Glu Gln Met Pro Lou Thr Pro Pro Arg Phe Asp His Asp Glu Gly Asp Gln Cys Ser Arg Leu Lou Glu Leu Val Lys Asp Ile Ser Ser His Leu Asp Val Thr Ala Leu Cys His Lys Ile Phe Lou His Ile His Gly Leu Ile Ser Ala Asp Arg Tyr Ser Lou Phe Lou Val Cys Glu Asp Ser Ser Asn Asp Lye Phe Leu Ile Ser Arg Leu Phe Asp Val Ala Glu Gly Ser Thr Leu Glu Glu Val Ser Asn Asn Cys Ile Arg Lou Glu Trp Aen Lye Gly Ile Val Gly His Val Ala Ala Lou Gly Glu Pro Lou Asn Ile Lys Asp Ala Tyr Glu Asp Pro Arg Pha Asn Ala Glu Val Aep Gln Ile Thr Gly Tyr Lys Thr Gin Ser Ile Lou Cys Met Pro Ile Lys Asn His Arg Glu Glu Val Val Gly Val Ala Gln Ala Ile Asn Lys Lys Ser Gly Asn Gly Gly Thr Phe Thr Glu Lys Asp Glu Lys Asp Phe Ala Ala Tyr Leu Ala Phe Cys Gly Ile Val Lou His Asn Ala Cln Leu Tyr Glu Thr Ser Leu Leu Glu Aen Lys Arg Asn Gln Val Leu Leu Asp Leu Ala Ser Leu Ile Phe Glu Glu Gln Gln Ser Leu Glu Val Ile Leu Lys Lys Ile Ala Ala Thr Ile Ile Ser Phe Met Gin Val Gin Lys Cys Thr Ile Phe Ile Val Asp Glu Asp Cys Ser Asp Ser Phe Ser Ser Val Phe His Met Glu Cys Glu Glu Leu Glu Lye Ser Ser Asp Thr Leu Thr Arg Glu His Asp Ala Asn Lys Ile Ann Tyr Met Tyr Ala Gln Tyr Val Lys Asn Thr Met Clu Pro Leu Asn Ile Pro Asp Val Ser Lys Asp Lye Arg Phe Pro Trp Thr Thr Glu Asn Thr Gly Aen Val Aen Gln Gin Cys Ile Arg Ser Leu Lou Cys Thr Pro Ile Lys Asn Gly Lys Lys Aen Lys Val Ile Gly Val Cys Gln Leu Val Asn Lye Met Glu Glu Asn Thr Gly Lye Val Lye Pro Phe Aen Arg Aen Asp Clu Gln Phe Lou Glu Ala Phe Val Ile Phe Cys Gly Leu Gly Ile Gln Ann Thr Gln Met Tyr Glu Ala Val Glu Arg Ala Met Ala Lys Gln Met Val Thr Leu Glu Val Lou Ser Tyr His Ala Ser Ala Ala Glu Glu Glu Thr Arg Glu Leu Gln Ser Lou Ala Ala Ala Val Val Pro Ser Ala Gln Thr Lou Lye Ile Thr Aep Phe Ser Phe Ser Aap Phe Glu Leu Ser Asp Leu Glu Thr Ala Lou Cys Thr Ile Arg Met Phe Thr Asp Leu Ann Lou Val Gln Ann Phe Cin Met Lye His Glu Val Leu Cys Arg Trp Ile Lou Ser Val Lys Lys Ann Tyr Arg Lys Ann Val Ala Tyr His Asn Trp Arg His Ala Phe Aen Thr Ala Gln Cys Met Phe Ala Ala Leu Lys Ala Gly Lys Ile Gln Asn Lys Leu Thr Asp Leu Glu Ile Leu Ala Leu Leu Ile Ala Ala Leu Ser His Asp Leu Asp His Arg Gly Val Asn Asn Ser Tyr Ile Gln Arg Ser Glu His Pro Leu Ala Gln Leu Tyr Cys His Ser Ile Met Glu His His His Phe Aep Cln Cys Leu Met Ile Leu Aen Ser Pro Gly Asn Gln Ile Leu Ser Gly Leu Ser Ile Glu Glu Tyr Lya Thr Thr Leu Lys Ile Ile Lys Gln Ala Ile Leu Ala Thr Asp Leu Ala Leu Tyr Ile Lys Arg Arg Gly Clu Phe Phe Glu Leu Ile Arg Lys Asn Gln Phe Asn Leu Glu Asp Pro His Gln Lye Glu Leu Phe Leu Ala Met Leu Met Thr Ala Cys Asp Leu Ser Ala Ile Thr Lys Pro Trp Pro Ile Gln Gln Arg Ile Ala Clu Leu Val Ala Thr Glu Phe Phe Asp Gln Gly Asp Arg Glu Arg Lys Glu Leu Asn Ile Glu Pro Thr Asp Leu Hnt Asn Arg Glu Lys Lys Asn Lys Ile Pro Ser Met Gln Val Gly Phe Ile Asp Ala Ile Cys Leu Gln Leu Tyr Glu Ala Leu Thr HisVal Ser Glu Asp Cys Phe Pro Leu Leu Asp Gly Cya Arg Lye Aen Arg Gln Lys Trp Cln Ala Leu Ala Glu Gln Gln Glu Lys Met Leu Ile Asn Gly Glu Ser Gly Gln Ala Lye Arg Asn (2) INFORMATION FOR SEQ ID NO:23:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 875 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE D$SCRIPTION: SEQ ID NO:23:
Met Glu Arg Ala Gly Pro Ser Phe Gly Gln Gln Arg Gln Gln Gln Gln Pro Gln Gln Gln Lys Gln Gln Gln Arg Asp Gln Asp Ser Val Glu Ala Trp Leu Aep Asp Hie Trp Asp Phe Thr Phe Ser Tyr Phe Val Arg Lye Ala Thr Arg Glu Met Val Asn Ala Trp Phe Ala Glu Arg Val His Thr Ile Pro Val Cy-s Lye Glu Gly Ile Arg Gly His Thr Glu Ser Cys Ser Cye Pro Leu Gln Gln Ser Pro Arg Ala Asp Ann Ser Val Pro Gly Thr Pro Thr Arg Lye Ile Ser Ala Ser Glu Phe Asp Arg Pro Leu Arg Pro Ile Val Val Lye Asp Ser Glu Gly Thr Val Ser Phe Letr Ser Asp Ser Clu Lys Lys Glu Gln Met Pro Leu Thr Pro Pro Arg Phe Asp His Asp Glu Gly Asp Gln Cys Ser Arg Leu Lou Glu Leu Val Lys Asp Ile Ser Ser His Leu Asp Val Thr Ala Leu Cys His Lys Ile Phe Lou His Ile His Gly Leu Ile Ser Ala Asp Arg Tyr Ser Leu Phe Leu Val Cys Glu Asp Ser Ser Aen Asp Lys Phe Leu Ile Ser Arg Leu Phe Asp Val Ala Glu Gly Ser Thr Leu Glu Glu Val Ser Asn Aen Cys Ile Arg Leu Glu Trp Asn Lys Gly Ile Val Gly His Val Ala Ala Leu Gly Glu Pro Leu Aen Ile Lys Asp Ala Tyr Glu Asp Pro Arg Phe Asn Ala Glu Val Asp Gin Ile Thr Gly Tyr Lys Thr Gln Ser Ile Lou Cye Met Pro Ile Lys Asn His Arg Glu Glu Val Val Gly Val Ala Gln Ala Ile Aen Lye Lye Ser Gly Asn Gly Gly Thr Phe Thr Glu Lys Asp Glu Lys Asp Phe Ala Ala Tyr Leu Ala Phe Cye Gly Ile Val Lou His Asn Ala Gln Lou Tyr Glu Thr Ser Leu Leu Glu Aen Lye Arg Aen Gln Val Leu Leu Asp Leu Ala Ser Leu Ile Phe Glu Glu Gln Gln Ser Leu Glu Val Ile Leu Lys Lys Ile Ala Ala Thr Ile Ile Ser Phe Met Gln Val Gln Lys Cye Thr lie Phe Ile Val Asp Glu Asp Cys Ser Asp Ser Phe Ser Ser Val Phe His Met Glu Cys Glu Glu Leu Glu Lys Ser Ser Asp Thr Leu Thr Arg Glu His Asp Ala Aen Lye Ile Aen Tyr Met Tyr Ala Gln Tyr Va1 Lys Asn Thr Met Glu Pro Leu Asn Ile Pro Asp Val Ser Lye Asp Lys Arg Phe Pro Trp Thr Thr Glu Asn Thr Gly Asn Val Asn Gln Gln Cys Ile Arg Ser Leu Leu Cys Thr Pro Ile Lys Asn Gly Lye Lys Asn Lye Val Ile Gly Val Cye Gln Lou Val Asn Lys Met Glu Glu Asn Thr Gly Lye Val Lye Pro Phe Aen Arg Asn Asp Glu Gln Phe Leu Glu Ala Phe Val Ile Phe Cys Gly Leu Gly Ile Gin Asn Thr Gln Met Tyr Giu Ala Val Glu Arg Ala Met Ala Lys Gln Met Val Thr Leu Glu Val Leu Ser Tyr His Ala Ser Ala Ala Glu Glu Glu Thr Arg Glu Lou Gln Ser Lou Ala Ala Ala Val Val Pro Ser Ala Gln Thr Leu Lys Ile Thr Asp Phe Ser Phe Ser Asp Phe Glu Leu Ser Asp Leu Glu Thr Ala Leu Cys Thr Ile Arg Met Phe Thr Asp Leu Aen Leu Val Gln Asn Phe Gln Met Lye His Glu Val Leu Cys Arg Trp Ile Leu Ser Val Lys Lys Aen Tyr Arg Lys Ann Val Ala Tyr His Asn Trp Arg His Ala Phe Asn Thr Ala Gin Cys Met Phe Ala Ala Leu Lys Ala Gly Lys I2e Gln Asn Lys Leu Thr Asp Leu Glu Ile Leu Ala Leu Leu lie Ala Ala Leu Ser His Asp Leu Asp His Arg Gly Val Aan Aen Ser Tyr Ile Gln Arg Ser Glu His Pro Leu Ala Gln Lou Tyr Cys His Ser Ile Met Glu His His His Phe Asp Gln Cys Leu Met Ile Leu Asn Ser Pro Gly Asn Gln Ile Leu Ser Gly Leu Ser Ile Glu Glu Tyr Lye Thr Thr Leu Lys Ile Ile Lys Gln Ala Ile Leu Ala Thr Asp Leu Ala Leu Tyr Ile Lye Arg Arg Gly Glu Phe Phe Glu Leu Ile Arg Lys Asn Gln Phe Asn Leu Glu Asp Pro His Gln Lys Glu Leu Phe Leu Ala Met Leu Met Thr Ala Cys Asp Leu Ser Ala Ile Thr Lys Pro Trp Pro Ile Gln Gln Arg Ile Ala Glu Leu Val Ala Thr Glu Phe Phe Asp Gln Gly Asp Arg Glu Arg Lys Glu Leu Asn Ile Glu Pro Thr Asp Leu Met Asn Arg Clu Lys Lys Asn Lye Ile Pro Ser Met Gln Val Gly Phe Ile Asp Ala Ile Cys Lou Gln Leu.Tyr Glu Ala Lou Thr His Val Ser Glu Asp Cys Phe Pro Leu Leu Asp Gly Cys Arg Lys Asn Arg Gln Lys Trp Gln Ala Lou Ala Glu Gln Gln Glu Lys Met Lou Ile Asn Gly Glu Ser Gly Gln Ala Lys Arg Asn Applrnats or agenrs filc 32083 ;ntcrnjtnms.rppi ~.
referenee nurnber ir/~! 1~ 4 06 0 6 6 I
INDICATIONS RELA"I'1NG'i'c) A I)I:POSI'fla) N11C1tOOItGANISNt (PCT Rule 13brs) A. The rndrarrons made beiow retate to the mrcroorCanrsm relerred to in inc u¾acrrprion on pape S hne 5 6-9 S. IDENTiFICATION OF DEPOSIT Funher deposrts are rdentiGcd on an addrtional sbeet ~
Name of deprustary institution American Type Culture Collection Address o( deposiury irrstitution liac"aa poual cele.al ra.uurr) 12301 Parklawn Drive Rockville, Maryland 20852 US
Oate of deposit .kcx wn Number 4 May.1993 ATCC 69296 C. ADDITIONAL INDICATIONS (ieope btaai r(em applicabkj This inforrnauon ts continued on an additional sbeet 0 "In respect of those designations in which a European patent is sought, a sample of the deposited microorganism will be made available until the publication of the mention of the grant of the European patent or until the date on which the application has been refused or withdracm or is deemed to be withdrawn, only by the issue of such a sample co an expert nominaced by the person requesting the sample (Rule 23(4) EPC)."
D. DESIGNATED STATES FOR wHICH INDICATIONS nRE ALADE riJ+l+rrrd+c.acw.rr wrJa .u/cnjrvad Uero, EP
E. SEPARATE FLJRNISSING OF INDICATIONS !leave blr.k 4.w.p06eable) Theindinttoasiistedbeiowwillbesubmrnedtotbe Imernauonai Uureauiater(speuiyree`eacra(oaareo/reerwiircrnowrcj.. 'Accasree Nrwbero/Deyoair ) For recerving Ot(iee use only For tntematronal Bureau use oniy This sbcct was received with the inlernational applrcauon r-i Ihis sheet +.=as rece:ved by the Intemauonal Bureau on Authonzed officer Autnurizcd ot:iccr 8roc' Form PCf/RO/134 (Juiy 1992)
(1) GENERAL INFORMATION:
(i) APPLICANT: The Board of Regents of the University of Washinqton (ii) TITLE OF INVENTION: Cyclic GMP-Binding, Cyclic GMP-Specific Phosphodiesterase Materials and Methods (iii) NUMBER OF SEQUENCES: 23 (iv) CORRESPONDENCE ADDRESS:
(A) ADDRESSEE: Marshall, O=Toole, Gerstein, Murray &
Borun (B) STREET: 6300 Sears Tower, 233 S. Wacker Drive (C) CITY: Chicago (D) STATE: Illinois (E) COUNTRY: USA
(F) ZIP: 60606 (v) COMPUTER READABLE FORM:
(A) MEDIUM TYPE: Floppy diek (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS
(D) SOFTWARE: PatentIn Release #1.0, Version #1.25 (vi) CURRENT APPLICATION DATA:
(A) APPLICATION NUMBER:
(B) FILING DATE:
(C) CLASSIFICATION:
(vii) PRIOR APPLICATION DATA:
(A) APPLICATION NUMBER: US 08/068,051 (B) FILING DATE: 27-MAY-1993 (viii) ATTORNEY/AGENT INFORMATION:
(A) NAME: Noland, Greta E.
(8) REGISTRATION NUMBER: 35,302 (C) REFERENCE/DOCRET NUMBBR: 32083 (ix) TELECOMMUNICATION INFORMATION:
(A) TELEPHONE: (312) 474-6300 (8) TELEFAX: (312) 474-0448 (C) TELEX: 25-3856 (2) INFORMATION FOR SEQ ID NO:l:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 20 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:
Arg Glu Xaa Asp Ala Asn Arg Ile Asn Tyr Met Tyr Ala Gln Tyr Val Lys Aen Thr Met (2) INFORMATION FOR SEQ ID NO:2:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 9 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:
Gln 5er Leu Ala Ala Ala Val Val Pro (2) INFORMATION FOR SEQ ID NO:3:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 8 amino acids (B) TYPE: amino acid (D) TOPOLOGYs linear (ii) MOLECULE TYPEs peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:
Phe Asp Asn Asp Glu Gly Glu Gin (2) INFORMATION FOR SEQ ID NO:4:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: D'NA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:
(2) INFORMATION FOR SEQ ID NO:5:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(iv) ANTI-SENSE: YES
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:
(2) INFORlSATION FOR SEQ ID NO:6 (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: aingle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:6 (2) INFORMATION FOR SEQ ID P0:7 (i) SEQUENCE CHARACTERISTICSs (A) LENGTH: 23 base pairs (B) TYPE,: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(iv) ANTI-SENSE: YES
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:7 (2) INFORMATION FOR SEQ ID NOs8 (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 36 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(iv) ANTI-SENSE: YES
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:8 (2) INFORMATION FOR SEQ ID N0:9 (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 4474 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: CDNA
(ix) E'E:ATURE:
(A) NAME/KEY: CDS
(B) LOCATION: 99..2723 (xi) SEQUENCE DESCRIPTION: SEQ ID N0:9 Met Glu Arg Ala Gly Pro Gly Cys Arg Ala Ala Ala Thr Ala Met Gly Pro Gly Leu Gly Arg Ser Val Ala Gly Arg Ser Leu Gly Leu Tyr Leu Leu Tyr Phe Val Arg Lys Gly Thr Arg Glu Met Val Asn Ala Trp Phe Ala Glu Arg Val His Thr Ile Pro Val Cys Lys Glu Gly Ile Lys Gly His Thr Glu Ser Cys Ser Cys Pro Leu Gin Pro Ser Pro Arg Ala Glu Ser Ser Val Pro Gly Thr Pro Thr Arg Lys Ile Ser Ala Ser Glu Phe Asp Arg Pro Leu Arg Pro Ile Val Ile Lys Asp Ser Glu Gly Thr Val Ser Phe Leu Ser Asp Ser Asp Lys Lys Glu Gln Met Pro Leu Thr Ser Pro Arg Phe Asp Asn = Asp Glu Gly Asp Gln Cys Ser Arg Leu Leu Glu Leu Val Lys Asp Ile Ser Ser His Leu Asp Val Thr Ala Leu Cys His Lys Ile Phe Leu His Ile His Gly Leu Ile Ser Ala Asp Arg Tyr'Ser Leu Phe Leu Val Cys Glu Asp Ser Ser Asn Asp Lys Phe Leu Ile Ser Arg Leu Phe Asp Val Ala Glu Gly Ser Thr Leu Glu Glu Ala Ser Asn Asn Cys Ile rlrg Leu Glu Trp Asn Lys Gly Ile Val Gly His Val Ala Ala ?he Gly Glu Pro Leu Asn Ile Lys Asp Ala Tyr Glu Asp Pro Arg Phe Asn Ala Glu Val Asp Gln Ile Thr Gly Tyr Lys Thr Gln Ser Ile Leu Cys Met Pro Ile 250 255 _ 260 Lys Asn His Arg Glu Glu Val Val Gly Val Ala Gln Ala Ile Asn Lys Lys Ser Gly Asn Gly Gly Thr Phe Thr Glu Lys Asp Glu Lys Asp Phe Ala Ala Tyr Leu Ala Phe Cys Gly I1e Val Leu His Asn Ala Gln Leu Tyr Glu Thr Ser Leu Leu Giu Asn Lys Arg Asn Gln Val Leu Leu Asp CTT GCT AGC TTA ATT TTT GAA GAA CAA CAA TCA TTA c,aA GTA ATT CTA 1121 Leu Ala Ser Leu Ile Phe Glu Glu Gln Gln Ser Leu Glu Val Ile Leu Arg Lys Ile Ala Ala Thr Ile Ile Ser Pro Met Gin Val Gln Lys Cys Thr Ile Phe Ile V,al Asp G1u Asp Cys Ser Asp Ser Phe Ser Ser Val Phe His Met Glu Cys Glu Glu Leu Glu Lys Ser Ser Asp Thr Leu Thr Arg Glu Arg Asp Ala Thr Arg Ile Asn Tyr Met Tyr Ala Gln Tyr Val 390 395 400 ' 405 Lys Asn Thr Met Glu Pro Leu Asn ILa Pro Asp Val Ser Lys Asp Lys Arg Phe Pro Trp Thr Asn Glu Asn Met Gly Asn Ile Asn Gln Gln Cys Ile Arq Ser Leu Leu Cys Thr Pro Ile Lys Asn Gly Lys Lys Asn Lys Val Ile Gly Val Cys Gln Leu Val Asn Lys Met Glu Glu Thr Thr Gly Lys Val Lys Ala Phe Aen Arg Aen Aep Glu Gln Phe Leu Glu Ala Phe Val Iie Phe Cys Gly Leu Gly Ile Gln Aen Thr Gin Met Tyr Glu Ala Val Glu Arg Ala Het Ala Lys Gln Met Val Thr Leu Glu Val Leu Ser Tyr His Ala Ser Ala Ala Glu Glu Glu Thr Arg Glu Leu Gln Ser Lou Ala Ala Ala Val Val Pro Ser Ala Gln Thr Leu Lye Ile Thr Asp Phe Ser Phe Ser Asp Phe Glu Leu Ser Asp Leu Glu Thr Ala Leu Cys Thr Ile Arg Met Phe Thr Asp Leu Asn Leu Val Gln Asn Phe Gln Met Lys His Glu Val Leu Cys Lye Trp Ile Leu Ser Val Lys Lys Aen Tyr Arg Lye Aen Val Ala Tyr His Aen Trp Arg His Ala Phe Asn Thr Ala Gln Cys Met Phe Ala Ala Leu Lys Ala Gly Lys Ile Gln Lys Arg Leu Thr Asp Leu Glu Ile Leu Ala Leu Leu Ile Ala Ala Leu Ser His Asp Leu Asp His Arg Gly Val Asn Asn Ser Tyr ile Gln Arg Ser Glu His Pro Leu Ala Gin Leu Tyr Cye His Ser Ile Met Glu His His His Phe Asp Gln Cys Leu Met Ile Leu Aen Ser Pro Gly Aen Gln Ile Leu Ser Gly Leu Ser Ile Glu Glu Tyr Lye Thr Thr Leu Lys Ile Ile Lye Gln Ala Ile Leu Ala Thr Asp Leu Ala Leu Tyr Ile Lys Arg Arg Gly Glu Phe Phe Glu Leu Ile Met Lye Aen Gln Phe Aen Leu Glu Asp Pro His Gln Lys Glu Leu Phe Leu Ala Met Leu Met Thr Ala Cys Asp Leu Ser Ala Ile Thr Lys Pro Trp Pro Ile Gln Gln Arg Ile Ala Glu Leu Val Ala Thr Glu Phe Phe Asp Gln Gly Asp Arg Glu Arg Lys Giu-Leu Asn Ile Glu Pro Ala Asp Leu Met Asn Arg Glu Lys Lys Asn Lys Ile Pro Ser Met Gln Val Gly Phe Ile Asp Ala Ile Cys Leu Gln Leu Tyr Glu Ala Leu Thr His Val Ser Glu Asp Cys Phe Pro Leu Leu Asp Gly Cys Arg Lys Asn Arg Gln Lys Trp Gln Ala Leu Ala Glu Gln Gln Glu Lys Thr CTG ATC AAT GGT GeIA AGC AGC CAG ACC AAC CGA CAG CAA CGG AAT TCC 2705 Leu Ile Asn Gly Glu Ser Ser Gin Thr Asn Arg Gln Gln Arg Asn Ser Val Ala Val Gly Thr Val GAATTCCCTT TAPrAATGTGT CAGACAGTAG CTTCTTGGGC CGGGTTGTTC CCGCAGCTCC 3173 (2) INFORMATION FOR SEQ ID NO:10:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 87S amino acids (8) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTIONs SEQ ID N0:10:
Met Glu Arg Ala Gly Pro Gly Cys Arg Ala Ala Ala Thr Ala Met Gly Pro Gly Leu Gly Arg Ser Val Ala Gly Arq Ser Leu Gly Leu Tyr Leu Leu Tyr Phe Val Arg Lys Gly Thr Arg Glu Met Val Asn Ala Trp Phe Ala Glu Arg Val His Thr Ile Pro Val Cys Lys Glu Gly Ile Lys Gly His Thr Glu Ser Cys Ser Cys Pro Leu Gln Pro Ser Pro Arg Ala Glu Ser Ser Val Pro Gly Thr Pro Thr Arg Lys Ile Ser Ala Ser Glu Phe Asp Arg Pro Leu Arg Pro Ile Val Ile Lys Asp Ser Glu Gly Thr Val Ser Phe Leu Ser Asp Ser Asp Lys Lys Glu Gln Met Pro Leu Thr Ser Pro Arg Phe Asp Asn Asp Glu Gly Asp Gln Cys Ser Arg Leu Leu Glu Leu Val Lys Asp Ile Ser Ser His Leu Asp Val Thr Ala Leu Cys His Lys Ile Phe Leu His Ile His Gly Leu Ile Ser Ala Asp Arg Tyr,Ser Lou Phe Leu Val Cys Glu Asp Ser Ser Asn Asp Lys Phe Leu Ile Ser Arg Leu Phe Asp Val Ala Glu Gly Ser Thr Leu Glu Glu Ala Ser Asn Asn Cys Ile Arg Leu Glu Trp Asn Lys Gly Ile Val Gly His Val Ala Ala Phe Gly Glu Pro Leu Asn Ile Lys Asp Ala Tyr Glu Asp Pro Arg Phe Asn Ala Glu Val Asp Gln Ile Thr Gly Tyr Lys Thr Gln Ser Ile Lou Cys Met Pro Ile Lys Asn His Arg Glu Glu Va1 Val Gly Val Ala Gln Ala Ile Asn Lys Lys Ser Gly Asn Gly Gly Thr Phe Thr Glu Lys Asp Glu Lys Asp Phe Ala Ala Tyr Lou Ala Phe Cys Gly Zle Val Lou His Asn Ala Gln Leu Tyr Glu Thr Ser Leu Lou Glu Asn Lys Arg Asn Gin Val Leu Lou Asp Lou Ala Ser Lou Ile Phe Clu Glu Gln Gln Ser Lou Glu Val Ile Leu Arg Lys Ile Ala Ala Thr Ile Ile Ser Pro Met Gln Val Gln Lys Cys Thr Ile Phe Ile Val Asp Glu Asp Cys Ser Asp Ser Phe Ser Ser Val Phe His Met Glu Cy^ Glu Glu Leu Glu Lys Ser Ser Asp Thr Leu Thr Arg Glu Arg Asp Ala Thr Arg Ile Asn Tyr Met Tyr Ala Gln Tyr Val Lys Asn Thr Met Glu Pro Lou Asn Ile Pro Asp Val Ser Lys Asp Lye Arg Phe Pro Trp Thr Asn Glu Asn Met Gly Asn Ile Asn Gln Gln Cys Ile Arg Ser Leu Leu Cys Thr Pro Ile Lys Asn Gly Lys Lys Asn Lys Val Ile Gly Val Cys Gin Leu Val Asn Lys Met Glu Glu Thr Thr Gly Lys Val Lys Ala Phe Asn Arg Asn Asp Glu Gln Phe Leu Glu Ala Phe Val Ile Phe Cys Gly Leu Gly Ile Gln Asn Thr Gln Met Tyr Glu Ala Val Glu Arg Ala Met Ala Lys Gln Met Val Thr Leu Glu Val Leu Ser Tyr His Ala Ser Ala Ala Glu Glu Glu Thr Arg Glu Leu Gln Ser Leu Ala Ala Ala Val Val Pro Ser Ala Gln Thr Leu Lys Ile Thr Asp Phe Ser Phe Ser Asp Phe Glu Lou Ser Asp Lou Glu Thr Ala Lou Cys Thr Ile Arg Met Phe Thr Asp Lou Asn Leu Val Gln Ann Phe Gln Met Lys His Glu Val Leu Cys Lys Trp Ile Leu Ser Val Lys Lys Asn Tyr Arg Lys Asn Val Ala Tyr His Asn Trp Arg His Ala Phe Ann Thr Ala Gln Cys Met Phe Ala Ala Lou Lys Ala Gly Lys Ile Gln Lye Arg Lou Thr Asp Leu Glu Ile Lou Ala Leu Lou Ile Ala Ala Leu Ser His Asp Leu Asp His Arg Gly Val Asn Asn Ser Tyr Ile Gln Arg Ser Glu His Pro Lou Ala Gln Leu Tyr Cys His Ser Ile Met Glu His His His Phe Asp Gin Cys Lou Met Ile Leu Asn Ser Pro Gly Asn Gln Ile Lou Ser Gly Lou Ser Ile Glu Glu Tyr Lys Thr Thr Lou Lys Ile Ile Lys Gln Ala Ile Leu Ala Thr Asp Leu Ala Leu Tyr Ile Lys Arg Arg Gly Glu Phe Phe Glu Leu Ile Met Lys Asn Gln Phe Asn Leu Glu Asp Pro His Gln Lys Glu Leu Phe Lou Ala Met Lou Met Thr Ala Cys Asp Lou Ser Ala Ile Thr Lys Pro Trp Pro Ile Gln Gin Arg Ile Ala Glu Leu Val Ala Thr Glu Phe Phe Asp Gln Gly Asp Arg Glu Arg Lys Glu Leu Asn Ile Glu Pro Ala Asp Lou Met Asn Arg Glu Lys Lys Asn Lys Ile Pro Ser Met Gln Val Gly Phe Ile Asp Ala Ile Cys Leu Gln Leu Tyr Glu Ala Leu Thr His Val Ser Glu Asp Cys Phe Pro Leu Leu Asp Gly Cys Arg Lys Aen Arg Gln Lys Trp Gln Ala Leu Ala Glu Gln Gln Glu Lys Thr Leu Ile Asn Gly Glu Ser Ser Gln Thr Asn Arg -5a Gln Gln Arg Asn Ser Val Ala Val Gly Thr Val (2) INFORMATION FOR SEQ ID NO:11:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 2060 baee paire (8) TYPE: nucleic acid (C) STRANDEDNESS: sinqle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:lls (2) INFORMATION FOR SEQ ID N0:12:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 1982 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) HOLECULE TYPE: cDNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:
GGTTCAATGC AGAAGTTGAC CAAATTACAG GCTACAAGAC AC7lAAGCATT CTTTGTATGC 300 AGTTTCTGGA AGCTTTTGTC ATCTTTTGTG GCTTGGGGAT'CCAGAACACG CAGATCTATG 1020 TGAAACATGA GGTTCTTTGC AGATGGATTT TAAGTGTTAA GAAGAATTAT CGGAAGAATG 1320*
(2) INFORMATION FOR SEQ ID N0:13:
(i) SEQUENCS CHARACTERISTICS:
(A) LENGTH: 18 base pairs (8) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:
(2) INFORMATION FOR SEQ ID NO:14:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 base pairs (B) TYPEs nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ( ii ) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:14:
(2) INFORMATION FOR SEQ ID NO:15:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:
(2) INFORMATION FOR SEQ ID N0:16:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPEt DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:16:
(2) INFORMATION FOR SEQ ID NO:17:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 1107 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: eDNA
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:17:
(2) INFORMATION FOR SEQ ID NO:18:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NOs18:
(2) INFORMATION FOR SEQ ID NO:19:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:19:
(2) INFORMATION FOR SEQ ID N0:20:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 30 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:20:
(2) INFORMATION FOR SEQ ID NO:21:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA
(xi) SEQUENCE DESCRIPTION: SEQ ID NOt21:
(2) INFORMATION FOR SEQ ID NO:22:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 2645 base pairs (8) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA
(ix) FEATURE:
(A) NAME/1CEY: CDS
(B) LOCATION: 12..2636 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22:
Met Glu Arg Ala Gly Pro Ser Phe Cly Gln Gln Arg Gln Gln Gin Gin Pro Gln Gln Gln Lys Gin Gln Gln Arg Asp Gln Asp Ser Val Glu Ala Trp Leu Asp Asp His Trp Asp Phe Thr Phe Ser Tyr Phe Val Arg Lys Ala Thr Arg Glu Met Val Asn Ala Trp PheAla Glu Arg Val His Thr Ile Pro Val Cys Lys Glu Gly Ile Arg Giy His Thr Glu Ser Cys Ser Cys Pro Leu Gln Gln Ser Pro Arg Ala Asp Asn Ser Val Pro Gly Thr Pro Thr Arg Lys Ile Ser Ala Ser Glu Phe Asp Arg Pro Leu Arg Pro Ile Val Val Lys Asp Ser Glu Gly Thr Val Ser Phe Leu 110 ~15 '20 - ~~-2 5 Ser Asp Ser Glu Lys Lye Glu Gln Met Pro Lou Thr Pro Pro Arg Phe Asp His Asp Glu Gly Asp Gln Cys Ser Arg Leu Lou Glu Leu Val Lys Asp Ile Ser Ser His Leu Asp Val Thr Ala Leu Cys His Lys Ile Phe Lou His Ile His Gly Leu Ile Ser Ala Asp Arg Tyr Ser Lou Phe Lou Val Cys Glu Asp Ser Ser Asn Asp Lye Phe Leu Ile Ser Arg Leu Phe Asp Val Ala Glu Gly Ser Thr Leu Glu Glu Val Ser Asn Asn Cys Ile Arg Lou Glu Trp Aen Lye Gly Ile Val Gly His Val Ala Ala Lou Gly Glu Pro Lou Asn Ile Lys Asp Ala Tyr Glu Asp Pro Arg Pha Asn Ala Glu Val Aep Gln Ile Thr Gly Tyr Lys Thr Gin Ser Ile Lou Cys Met Pro Ile Lys Asn His Arg Glu Glu Val Val Gly Val Ala Gln Ala Ile Asn Lys Lys Ser Gly Asn Gly Gly Thr Phe Thr Glu Lys Asp Glu Lys Asp Phe Ala Ala Tyr Leu Ala Phe Cys Gly Ile Val Lou His Asn Ala Cln Leu Tyr Glu Thr Ser Leu Leu Glu Aen Lys Arg Asn Gln Val Leu Leu Asp Leu Ala Ser Leu Ile Phe Glu Glu Gln Gln Ser Leu Glu Val Ile Leu Lys Lys Ile Ala Ala Thr Ile Ile Ser Phe Met Gin Val Gin Lys Cys Thr Ile Phe Ile Val Asp Glu Asp Cys Ser Asp Ser Phe Ser Ser Val Phe His Met Glu Cys Glu Glu Leu Glu Lye Ser Ser Asp Thr Leu Thr Arg Glu His Asp Ala Asn Lys Ile Ann Tyr Met Tyr Ala Gln Tyr Val Lys Asn Thr Met Clu Pro Leu Asn Ile Pro Asp Val Ser Lys Asp Lye Arg Phe Pro Trp Thr Thr Glu Asn Thr Gly Aen Val Aen Gln Gin Cys Ile Arg Ser Leu Lou Cys Thr Pro Ile Lys Asn Gly Lys Lys Aen Lys Val Ile Gly Val Cys Gln Leu Val Asn Lye Met Glu Glu Asn Thr Gly Lye Val Lye Pro Phe Aen Arg Aen Asp Clu Gln Phe Lou Glu Ala Phe Val Ile Phe Cys Gly Leu Gly Ile Gln Ann Thr Gln Met Tyr Glu Ala Val Glu Arg Ala Met Ala Lys Gln Met Val Thr Leu Glu Val Lou Ser Tyr His Ala Ser Ala Ala Glu Glu Glu Thr Arg Glu Leu Gln Ser Lou Ala Ala Ala Val Val Pro Ser Ala Gln Thr Lou Lye Ile Thr Aep Phe Ser Phe Ser Aap Phe Glu Leu Ser Asp Leu Glu Thr Ala Lou Cys Thr Ile Arg Met Phe Thr Asp Leu Ann Lou Val Gln Ann Phe Cin Met Lye His Glu Val Leu Cys Arg Trp Ile Lou Ser Val Lys Lys Ann Tyr Arg Lys Ann Val Ala Tyr His Asn Trp Arg His Ala Phe Aen Thr Ala Gln Cys Met Phe Ala Ala Leu Lys Ala Gly Lys Ile Gln Asn Lys Leu Thr Asp Leu Glu Ile Leu Ala Leu Leu Ile Ala Ala Leu Ser His Asp Leu Asp His Arg Gly Val Asn Asn Ser Tyr Ile Gln Arg Ser Glu His Pro Leu Ala Gln Leu Tyr Cys His Ser Ile Met Glu His His His Phe Aep Cln Cys Leu Met Ile Leu Aen Ser Pro Gly Asn Gln Ile Leu Ser Gly Leu Ser Ile Glu Glu Tyr Lya Thr Thr Leu Lys Ile Ile Lys Gln Ala Ile Leu Ala Thr Asp Leu Ala Leu Tyr Ile Lys Arg Arg Gly Clu Phe Phe Glu Leu Ile Arg Lys Asn Gln Phe Asn Leu Glu Asp Pro His Gln Lye Glu Leu Phe Leu Ala Met Leu Met Thr Ala Cys Asp Leu Ser Ala Ile Thr Lys Pro Trp Pro Ile Gln Gln Arg Ile Ala Clu Leu Val Ala Thr Glu Phe Phe Asp Gln Gly Asp Arg Glu Arg Lys Glu Leu Asn Ile Glu Pro Thr Asp Leu Hnt Asn Arg Glu Lys Lys Asn Lys Ile Pro Ser Met Gln Val Gly Phe Ile Asp Ala Ile Cys Leu Gln Leu Tyr Glu Ala Leu Thr HisVal Ser Glu Asp Cys Phe Pro Leu Leu Asp Gly Cya Arg Lye Aen Arg Gln Lys Trp Cln Ala Leu Ala Glu Gln Gln Glu Lys Met Leu Ile Asn Gly Glu Ser Gly Gln Ala Lye Arg Asn (2) INFORMATION FOR SEQ ID NO:23:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 875 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE D$SCRIPTION: SEQ ID NO:23:
Met Glu Arg Ala Gly Pro Ser Phe Gly Gln Gln Arg Gln Gln Gln Gln Pro Gln Gln Gln Lys Gln Gln Gln Arg Asp Gln Asp Ser Val Glu Ala Trp Leu Aep Asp Hie Trp Asp Phe Thr Phe Ser Tyr Phe Val Arg Lye Ala Thr Arg Glu Met Val Asn Ala Trp Phe Ala Glu Arg Val His Thr Ile Pro Val Cy-s Lye Glu Gly Ile Arg Gly His Thr Glu Ser Cys Ser Cye Pro Leu Gln Gln Ser Pro Arg Ala Asp Ann Ser Val Pro Gly Thr Pro Thr Arg Lye Ile Ser Ala Ser Glu Phe Asp Arg Pro Leu Arg Pro Ile Val Val Lye Asp Ser Glu Gly Thr Val Ser Phe Letr Ser Asp Ser Clu Lys Lys Glu Gln Met Pro Leu Thr Pro Pro Arg Phe Asp His Asp Glu Gly Asp Gln Cys Ser Arg Leu Lou Glu Leu Val Lys Asp Ile Ser Ser His Leu Asp Val Thr Ala Leu Cys His Lys Ile Phe Lou His Ile His Gly Leu Ile Ser Ala Asp Arg Tyr Ser Leu Phe Leu Val Cys Glu Asp Ser Ser Aen Asp Lys Phe Leu Ile Ser Arg Leu Phe Asp Val Ala Glu Gly Ser Thr Leu Glu Glu Val Ser Asn Aen Cys Ile Arg Leu Glu Trp Asn Lys Gly Ile Val Gly His Val Ala Ala Leu Gly Glu Pro Leu Aen Ile Lys Asp Ala Tyr Glu Asp Pro Arg Phe Asn Ala Glu Val Asp Gin Ile Thr Gly Tyr Lys Thr Gln Ser Ile Lou Cye Met Pro Ile Lys Asn His Arg Glu Glu Val Val Gly Val Ala Gln Ala Ile Aen Lye Lye Ser Gly Asn Gly Gly Thr Phe Thr Glu Lys Asp Glu Lys Asp Phe Ala Ala Tyr Leu Ala Phe Cye Gly Ile Val Lou His Asn Ala Gln Lou Tyr Glu Thr Ser Leu Leu Glu Aen Lye Arg Aen Gln Val Leu Leu Asp Leu Ala Ser Leu Ile Phe Glu Glu Gln Gln Ser Leu Glu Val Ile Leu Lys Lys Ile Ala Ala Thr Ile Ile Ser Phe Met Gln Val Gln Lys Cye Thr lie Phe Ile Val Asp Glu Asp Cys Ser Asp Ser Phe Ser Ser Val Phe His Met Glu Cys Glu Glu Leu Glu Lys Ser Ser Asp Thr Leu Thr Arg Glu His Asp Ala Aen Lye Ile Aen Tyr Met Tyr Ala Gln Tyr Va1 Lys Asn Thr Met Glu Pro Leu Asn Ile Pro Asp Val Ser Lye Asp Lys Arg Phe Pro Trp Thr Thr Glu Asn Thr Gly Asn Val Asn Gln Gln Cys Ile Arg Ser Leu Leu Cys Thr Pro Ile Lys Asn Gly Lye Lys Asn Lye Val Ile Gly Val Cye Gln Lou Val Asn Lys Met Glu Glu Asn Thr Gly Lye Val Lye Pro Phe Aen Arg Asn Asp Glu Gln Phe Leu Glu Ala Phe Val Ile Phe Cys Gly Leu Gly Ile Gin Asn Thr Gln Met Tyr Giu Ala Val Glu Arg Ala Met Ala Lys Gln Met Val Thr Leu Glu Val Leu Ser Tyr His Ala Ser Ala Ala Glu Glu Glu Thr Arg Glu Lou Gln Ser Lou Ala Ala Ala Val Val Pro Ser Ala Gln Thr Leu Lys Ile Thr Asp Phe Ser Phe Ser Asp Phe Glu Leu Ser Asp Leu Glu Thr Ala Leu Cys Thr Ile Arg Met Phe Thr Asp Leu Aen Leu Val Gln Asn Phe Gln Met Lye His Glu Val Leu Cys Arg Trp Ile Leu Ser Val Lys Lys Aen Tyr Arg Lys Ann Val Ala Tyr His Asn Trp Arg His Ala Phe Asn Thr Ala Gin Cys Met Phe Ala Ala Leu Lys Ala Gly Lys I2e Gln Asn Lys Leu Thr Asp Leu Glu Ile Leu Ala Leu Leu lie Ala Ala Leu Ser His Asp Leu Asp His Arg Gly Val Aan Aen Ser Tyr Ile Gln Arg Ser Glu His Pro Leu Ala Gln Lou Tyr Cys His Ser Ile Met Glu His His His Phe Asp Gln Cys Leu Met Ile Leu Asn Ser Pro Gly Asn Gln Ile Leu Ser Gly Leu Ser Ile Glu Glu Tyr Lye Thr Thr Leu Lys Ile Ile Lys Gln Ala Ile Leu Ala Thr Asp Leu Ala Leu Tyr Ile Lye Arg Arg Gly Glu Phe Phe Glu Leu Ile Arg Lys Asn Gln Phe Asn Leu Glu Asp Pro His Gln Lys Glu Leu Phe Leu Ala Met Leu Met Thr Ala Cys Asp Leu Ser Ala Ile Thr Lys Pro Trp Pro Ile Gln Gln Arg Ile Ala Glu Leu Val Ala Thr Glu Phe Phe Asp Gln Gly Asp Arg Glu Arg Lys Glu Leu Asn Ile Glu Pro Thr Asp Leu Met Asn Arg Clu Lys Lys Asn Lye Ile Pro Ser Met Gln Val Gly Phe Ile Asp Ala Ile Cys Lou Gln Leu.Tyr Glu Ala Lou Thr His Val Ser Glu Asp Cys Phe Pro Leu Leu Asp Gly Cys Arg Lys Asn Arg Gln Lys Trp Gln Ala Lou Ala Glu Gln Gln Glu Lys Met Lou Ile Asn Gly Glu Ser Gly Gln Ala Lys Arg Asn Applrnats or agenrs filc 32083 ;ntcrnjtnms.rppi ~.
referenee nurnber ir/~! 1~ 4 06 0 6 6 I
INDICATIONS RELA"I'1NG'i'c) A I)I:POSI'fla) N11C1tOOItGANISNt (PCT Rule 13brs) A. The rndrarrons made beiow retate to the mrcroorCanrsm relerred to in inc u¾acrrprion on pape S hne 5 6-9 S. IDENTiFICATION OF DEPOSIT Funher deposrts are rdentiGcd on an addrtional sbeet ~
Name of deprustary institution American Type Culture Collection Address o( deposiury irrstitution liac"aa poual cele.al ra.uurr) 12301 Parklawn Drive Rockville, Maryland 20852 US
Oate of deposit .kcx wn Number 4 May.1993 ATCC 69296 C. ADDITIONAL INDICATIONS (ieope btaai r(em applicabkj This inforrnauon ts continued on an additional sbeet 0 "In respect of those designations in which a European patent is sought, a sample of the deposited microorganism will be made available until the publication of the mention of the grant of the European patent or until the date on which the application has been refused or withdracm or is deemed to be withdrawn, only by the issue of such a sample co an expert nominaced by the person requesting the sample (Rule 23(4) EPC)."
D. DESIGNATED STATES FOR wHICH INDICATIONS nRE ALADE riJ+l+rrrd+c.acw.rr wrJa .u/cnjrvad Uero, EP
E. SEPARATE FLJRNISSING OF INDICATIONS !leave blr.k 4.w.p06eable) Theindinttoasiistedbeiowwillbesubmrnedtotbe Imernauonai Uureauiater(speuiyree`eacra(oaareo/reerwiircrnowrcj.. 'Accasree Nrwbero/Deyoair ) For recerving Ot(iee use only For tntematronal Bureau use oniy This sbcct was received with the inlernational applrcauon r-i Ihis sheet +.=as rece:ved by the Intemauonal Bureau on Authonzed officer Autnurizcd ot:iccr 8roc' Form PCf/RO/134 (Juiy 1992)
Claims
1. An antisense polynucleotide consisting of a polynucleotide specific for the cGB-PDE
polynucleotide set out in SEQ ID NO:22.
polynucleotide set out in SEQ ID NO:22.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US6805193A | 1993-05-27 | 1993-05-27 | |
| US08/068,051 | 1993-05-27 | ||
| CA2363537A CA2363537C (en) | 1993-05-27 | 1994-05-27 | Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2363537A Division CA2363537C (en) | 1993-05-27 | 1994-05-27 | Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2698716A1 true CA2698716A1 (en) | 1994-12-08 |
Family
ID=22080121
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002141060A Expired - Fee Related CA2141060C (en) | 1993-05-27 | 1994-05-27 | Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods |
| CA2698716A Abandoned CA2698716A1 (en) | 1993-05-27 | 1994-05-27 | Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods |
| CA2363537A Expired - Fee Related CA2363537C (en) | 1993-05-27 | 1994-05-27 | Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002141060A Expired - Fee Related CA2141060C (en) | 1993-05-27 | 1994-05-27 | Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2363537A Expired - Fee Related CA2363537C (en) | 1993-05-27 | 1994-05-27 | Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods |
Country Status (13)
| Country | Link |
|---|---|
| US (7) | US5702936A (en) |
| EP (2) | EP0652960B9 (en) |
| JP (3) | JP4150070B2 (en) |
| AT (2) | ATE438723T1 (en) |
| CA (3) | CA2141060C (en) |
| DE (3) | DE69426804D1 (en) |
| DK (2) | DK1038963T3 (en) |
| ES (2) | ES2156898T3 (en) |
| GR (1) | GR3035616T3 (en) |
| HK (1) | HK1013427A1 (en) |
| LV (1) | LV12814B (en) |
| PT (2) | PT1038963E (en) |
| WO (1) | WO1994028144A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060222647A1 (en) * | 1993-05-27 | 2006-10-05 | Beavo Joseph A | Methods and compositions for modulating the activity of PDE5 |
| US5858694A (en) * | 1997-05-30 | 1999-01-12 | Cell Pathways, Inc. | Method for identifying compounds for inhibition of cancerous lesions |
| JP2002504341A (en) * | 1998-02-23 | 2002-02-12 | アイコス コーポレイション | Phosphodiesterase 10 |
| AU4654299A (en) * | 1998-07-21 | 2000-02-14 | Takeda Chemical Industries Ltd. | Novel protein and its dna |
| US6875575B1 (en) | 1998-11-25 | 2005-04-05 | Osi Pharmaceuticals, Inc. | Diagnostic methods for neoplasia |
| WO2000033067A1 (en) * | 1998-11-25 | 2000-06-08 | Cell Pathways, Inc. | Method for diagnosing neoplasia |
| US6100037A (en) * | 1999-01-07 | 2000-08-08 | Incyte Pharmaceuticals, Inc. | Human cyclic nucleotide PDEs |
| US6451807B1 (en) | 1999-04-30 | 2002-09-17 | Lilly Icos, Llc. | Methods of treating sexual dysfunction in an individual suffering from a retinal disease, class 1 congestive heart failure, or myocardial infarction using a PDE5 inhibitor |
| US6146876A (en) * | 1999-06-11 | 2000-11-14 | Millennium Pharmaceuticals, Inc. | 22025, a novel human cyclic nucleotide phosphodiesterase |
| US7098015B2 (en) * | 1999-06-11 | 2006-08-29 | Millennium Pharmaceuticals, Inc. | 27875, 22025, 27420, 17906, 16319, 55092 and 10218 molecules and uses therefor |
| GB9922125D0 (en) | 1999-09-17 | 1999-11-17 | Pfizer Ltd | Phosphodiesterase enzymes |
| CN1297049A (en) * | 1999-11-23 | 2001-05-30 | 上海博容基因开发有限公司 | Polypeptide-human cyclophosphate guanine suppressed phosphodiesterase 17 and polynucleotide for coding said polypeptide |
| JP2004500425A (en) | 2000-04-19 | 2004-01-08 | リリー アイコス リミテッド ライアビリティ カンパニー | Use of cyclic GMP-specific phosphodiesterase inhibitors for the treatment of Parkinson's disease |
| GB0016009D0 (en) * | 2000-06-29 | 2000-08-23 | Pfizer Ltd | Target |
| US6794192B2 (en) | 2000-06-29 | 2004-09-21 | Pfizer Inc. | Target |
| US6846813B2 (en) * | 2000-06-30 | 2005-01-25 | Pharmacia & Upjohn Company | Compounds to treat alzheimer's disease |
| US20030096864A1 (en) * | 2000-06-30 | 2003-05-22 | Fang Lawrence Y. | Compounds to treat alzheimer's disease |
| DK1317564T3 (en) * | 2000-09-14 | 2007-12-03 | Sinai School Medicine | Screening Methods to Identify G Proteins and Other Compounds That Modulate Phosphodiesterase (PDE) Activity |
| US20040219632A1 (en) * | 2001-04-20 | 2004-11-04 | Robert Margolskee | T1r3 a novel taste receptor |
| US7803982B2 (en) | 2001-04-20 | 2010-09-28 | The Mount Sinai School Of Medicine Of New York University | T1R3 transgenic animals, cells and related methods |
| US20070213407A1 (en) * | 2001-06-29 | 2007-09-13 | Elan Pharmaceuticals And Pharmacia & Upjohn Company Llc | Compounds to treat Alzheimer's disease |
| JP4999255B2 (en) | 2001-07-10 | 2012-08-15 | レイクウッド−アメディックス，インコーポレーテッド | Oligonucleotide-containing pharmacological composition and use thereof |
| GB0126417D0 (en) * | 2001-11-02 | 2002-01-02 | Pfizer Ltd | Crystal structure |
| US20050202549A1 (en) * | 2001-11-02 | 2005-09-15 | Pfizer Inc | Crystal structure |
| US7304128B2 (en) * | 2002-06-04 | 2007-12-04 | E.I. Du Pont De Nemours And Company | Carbon nanotube binding peptides |
| BRPI0409931A (en) * | 2003-05-01 | 2006-04-25 | Pfizer | pde5 crystal, its crystal structure and its in drug project |
| JP2010004758A (en) * | 2008-06-24 | 2010-01-14 | Probex Inc | Cyclic gmp detection method |
| US8841104B2 (en) | 2010-04-21 | 2014-09-23 | Nanomr, Inc. | Methods for isolating a target analyte from a heterogeneous sample |
| US20110262989A1 (en) | 2010-04-21 | 2011-10-27 | Nanomr, Inc. | Isolating a target analyte from a body fluid |
| US9476812B2 (en) | 2010-04-21 | 2016-10-25 | Dna Electronics, Inc. | Methods for isolating a target analyte from a heterogeneous sample |
| US9434940B2 (en) | 2012-12-19 | 2016-09-06 | Dna Electronics, Inc. | Methods for universal target capture |
| US9804069B2 (en) | 2012-12-19 | 2017-10-31 | Dnae Group Holdings Limited | Methods for degrading nucleic acid |
| US10000557B2 (en) | 2012-12-19 | 2018-06-19 | Dnae Group Holdings Limited | Methods for raising antibodies |
| US9551704B2 (en) | 2012-12-19 | 2017-01-24 | Dna Electronics, Inc. | Target detection |
| US9599610B2 (en) | 2012-12-19 | 2017-03-21 | Dnae Group Holdings Limited | Target capture system |
| US9995742B2 (en) | 2012-12-19 | 2018-06-12 | Dnae Group Holdings Limited | Sample entry |
| KR101550217B1 (en) * | 2014-03-26 | 2015-09-04 | 전남대학교산학협력단 | Recombinant vector for foreign gene expression without biological circuit interference of host cell and uses thereof |
| WO2017094885A1 (en) * | 2015-12-04 | 2017-06-08 | 国立大学法人東京大学 | Ligand fluorescent sensor protein and use thereof |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1290210B (en) * | 1962-12-04 | 1969-03-06 | Siemens Ag | Device for machine laying and connecting switching wires |
| US4129349A (en) * | 1975-11-10 | 1978-12-12 | Bell Telephone Laboratories, Incorporated | Quick-connect breadboarding system |
| JPH0797015B2 (en) * | 1988-10-28 | 1995-10-18 | 株式会社ピーエフユー | Position correction method of SMD by image processing |
| US5098890A (en) * | 1988-11-07 | 1992-03-24 | Temple University-Of The Commonwealth System Of Higher Education | Antisence oligonucleotides to c-myb proto-oncogene and uses thereof |
| JPH038400A (en) * | 1989-06-06 | 1991-01-16 | Sharp Corp | Positional correction of printed substrate |
| US4958013A (en) * | 1989-06-06 | 1990-09-18 | Northwestern University | Cholesteryl modified oligonucleotides |
| US5015637A (en) * | 1990-05-25 | 1991-05-14 | Hoechst-Roussel Pharmaceutical Inc. | Pyrido[3,4-b]pyrrolo[1,2-e][1,4,5]oxadiazepines |
| US5389527A (en) * | 1991-04-19 | 1995-02-14 | The Board Of Regents Of The University Of Washington | DNA encoding mammalian phosphodiesterases |
| WO1993005182A1 (en) * | 1991-09-05 | 1993-03-18 | Isis Pharmaceuticals, Inc. | Determination of oligonucleotides for therapeutics, diagnostics and research reagents |
| US6403302B1 (en) * | 1992-09-17 | 2002-06-11 | California Institute Of Technology | Methods and compositions for triple helix formation |
-
1994
- 1994-05-27 AT AT00112074T patent/ATE438723T1/en not_active IP Right Cessation
- 1994-05-27 DK DK00112074T patent/DK1038963T3/en active
- 1994-05-27 JP JP50101295A patent/JP4150070B2/en not_active Expired - Fee Related
- 1994-05-27 CA CA002141060A patent/CA2141060C/en not_active Expired - Fee Related
- 1994-05-27 EP EP94919288A patent/EP0652960B9/en not_active Expired - Lifetime
- 1994-05-27 WO PCT/US1994/006066 patent/WO1994028144A1/en active IP Right Grant
- 1994-05-27 AT AT94919288T patent/ATE199569T1/en not_active IP Right Cessation
- 1994-05-27 US US08/250,847 patent/US5702936A/en not_active Expired - Lifetime
- 1994-05-27 ES ES94919288T patent/ES2156898T3/en not_active Expired - Lifetime
- 1994-05-27 DK DK94919288T patent/DK0652960T3/en active
- 1994-05-27 PT PT00112074T patent/PT1038963E/en unknown
- 1994-05-27 DE DE69426804A patent/DE69426804D1/en not_active Expired - Lifetime
- 1994-05-27 DE DE69426804T patent/DE69426804T4/en not_active Expired - Lifetime
- 1994-05-27 ES ES00112074T patent/ES2333691T3/en not_active Expired - Lifetime
- 1994-05-27 CA CA2698716A patent/CA2698716A1/en not_active Abandoned
- 1994-05-27 PT PT94919288T patent/PT652960E/en unknown
- 1994-05-27 EP EP00112074A patent/EP1038963B1/en not_active Expired - Lifetime
- 1994-05-27 CA CA2363537A patent/CA2363537C/en not_active Expired - Fee Related
- 1994-05-27 DE DE69435227T patent/DE69435227D1/en not_active Expired - Lifetime
-
1995
- 1995-06-05 US US08/463,949 patent/US5955583A/en not_active Expired - Lifetime
- 1995-06-05 US US08/464,410 patent/US6037119A/en not_active Expired - Lifetime
- 1995-06-07 US US08/480,547 patent/US5652131A/en not_active Expired - Lifetime
-
1998
- 1998-12-21 HK HK98114207A patent/HK1013427A1/en not_active IP Right Cessation
-
2001
- 2001-03-21 GR GR20010400462T patent/GR3035616T3/en unknown
- 2001-12-12 LV LVP-01-167A patent/LV12814B/en unknown
-
2002
- 2002-04-03 US US10/115,515 patent/US20030054992A1/en not_active Abandoned
-
2003
- 2003-01-28 US US10/353,575 patent/US20050196833A1/en not_active Abandoned
-
2005
- 2005-05-24 JP JP2005150760A patent/JP4450767B2/en not_active Expired - Fee Related
- 2005-12-21 US US11/315,036 patent/US20060216809A1/en not_active Abandoned
-
2009
- 2009-11-25 JP JP2009267135A patent/JP2010042035A/en active Pending
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| Date | Code | Title | Description |
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| EEER | Examination request | ||
| FZDE | Discontinued |