CA2619841A1 - Methods for identifying merle mutations in the silv gene - Google Patents
Methods for identifying merle mutations in the silv gene Download PDFInfo
- Publication number
- CA2619841A1 CA2619841A1 CA002619841A CA2619841A CA2619841A1 CA 2619841 A1 CA2619841 A1 CA 2619841A1 CA 002619841 A CA002619841 A CA 002619841A CA 2619841 A CA2619841 A CA 2619841A CA 2619841 A1 CA2619841 A1 CA 2619841A1
- Authority
- CA
- Canada
- Prior art keywords
- gene
- human
- merle
- dog
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Abstract
Animals with mutations in SILV present with the merle coat color pattern phenotype. Methods for the identification of animals that harbor a mutation in the SILV gene are described. Mutations in the SILV gene can be identified from any biological sample such as a cell or tissue that contains genomic DNA. A
microsatellite marker identified using linkage disequilibrium mapping that segregates with merle is also described.
microsatellite marker identified using linkage disequilibrium mapping that segregates with merle is also described.
Claims (15)
1. A method for identifying individuals having mutations in the SILV gene comprising:
a) obtaining a biological sample from a dog or human; and b) analyzing the biological sample for mutations within the SILV
gene or human equivalent.
a) obtaining a biological sample from a dog or human; and b) analyzing the biological sample for mutations within the SILV
gene or human equivalent.
2. The method of claim 1 wherein the organism has a merle allele.
3. The method of claim 2 wherein the merle allele is SEQ ID
NO: 25 or a sequence hybridizing thereto under stringent conditions.
NO: 25 or a sequence hybridizing thereto under stringent conditions.
4. The method of claim 1 wherein the dog has a cryptic merle allele.
5. The method of claim 4 wherein the cryptic merle allele is SEQ ID NO: 34, 35 or 36 or a sequence hybridizing thereto under stringent conditions.
6. The method of claim 1 wherein the biological sample is cell or tissue containing genomic DNA obtained from a dog.
7. The method of claim 1 wherein the biological sample is analyzed by performing PCR using genomic DNA templates and polyacrylamide gel electrophoresis.
8. The method of claim 1 for screening a human for defects in the analogous human gene.
9. The method of claim 8 wherein the human has or is at risk of producing children with auditory or visual defects.
10. A method to identify a gene causing auditory or visual defects in a human comprising obtaining samples from WS 2 affected patients and normal individuals, and sequencing the DNA to identify any nonsense, missense, insertion, deletion, or splicing mutations in the samples in a region analogous to the SILV gene which are present in the WS 2 affected patients but not the normal individuals.
11. SEQ ID NO: 25 or a sequence hybridizing thereto under stringent conditions.
12. SEQ ID NO: 34, 35 or 36 or a sequence hybridizing thereto under stringent conditions.
13. A kit for screening a dog or human for the presence of the merle gene or analogous defects in a human gene comprising SEQ ID NO:
25 or a sequence hybridizing thereto under stringent conditions and reagents for determining the presence of the merle gene or analogous human gene in a sample obtained from the human or dog.
25 or a sequence hybridizing thereto under stringent conditions and reagents for determining the presence of the merle gene or analogous human gene in a sample obtained from the human or dog.
14. A kit for screening a dog or human for the presence of the merle gene or analogous defects in a human gene comprising SEQ ID NO:
25 or a sequence hybridizing thereto under stringent conditions and reagents for determining the presence of the merle gene or analogous human gene in a sample obtained from the dog or human.
25 or a sequence hybridizing thereto under stringent conditions and reagents for determining the presence of the merle gene or analogous human gene in a sample obtained from the dog or human.
15. A kit for screening a dog or human for the presence of the cryptic merle gene or analogous defects in a human gene comprising SEQ ID
NO: 34, 35 or 36 or a sequence hybridizing thereto under stringent conditions and reagents for determining the presence of the cryptic merle gene in a sample obtained from the dog or human.
NO: 34, 35 or 36 or a sequence hybridizing thereto under stringent conditions and reagents for determining the presence of the cryptic merle gene in a sample obtained from the dog or human.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US70858905P | 2005-08-16 | 2005-08-16 | |
| US60/708,589 | 2005-08-16 | ||
| PCT/US2006/032100 WO2007022335A1 (en) | 2005-08-16 | 2006-08-16 | Methods for identification of merle gene |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2619841A1 true CA2619841A1 (en) | 2007-02-22 |
| CA2619841C CA2619841C (en) | 2012-07-03 |
Family
ID=37501945
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2619841A Active CA2619841C (en) | 2005-08-16 | 2006-08-16 | Methods for identifying merle mutations in the silv gene |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7807370B2 (en) |
| EP (1) | EP1945803B1 (en) |
| JP (1) | JP5203202B2 (en) |
| CN (1) | CN101356285A (en) |
| AU (1) | AU2006279420B2 (en) |
| CA (1) | CA2619841C (en) |
| DE (1) | DE06801706T1 (en) |
| WO (1) | WO2007022335A1 (en) |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL63907A0 (en) | 1980-09-24 | 1981-12-31 | Cetus Corp | Diagnostic method and antibody probe for use therein |
| DE3381518D1 (en) | 1982-01-22 | 1990-06-07 | Cetus Corp | METHOD FOR CHARACTERIZING HLA AND THE CDNS TEST AGENTS USED IN IT. |
| US4582788A (en) | 1982-01-22 | 1986-04-15 | Cetus Corporation | HLA typing method and cDNA probes used therein |
| GB8311018D0 (en) | 1983-04-22 | 1983-05-25 | Amersham Int Plc | Detecting mutations in dna |
| US4683194A (en) | 1984-05-29 | 1987-07-28 | Cetus Corporation | Method for detection of polymorphic restriction sites and nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| CA1284931C (en) | 1986-03-13 | 1991-06-18 | Henry A. Erlich | Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids |
| CA1338457C (en) | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
| US5202231A (en) | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
| GB8810400D0 (en) | 1988-05-03 | 1988-06-08 | Southern E | Analysing polynucleotide sequences |
| US4988617A (en) | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
| US5424186A (en) | 1989-06-07 | 1995-06-13 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| FR2650840B1 (en) | 1989-08-11 | 1991-11-29 | Bertin & Cie | RAPID DETECTION AND / OR IDENTIFICATION OF A SINGLE BASED ON A NUCLEIC ACID SEQUENCE, AND ITS APPLICATIONS |
| ES2294777T3 (en) | 1990-05-03 | 2008-04-01 | Cornell Research Foundation, Inc. | DNA AMPLIFICATION SYSTEM THROUGH THERMOSTABLE LIGASE FOR THE DETECTION OF GENETIC DISEASES. |
| EP0834576B1 (en) | 1990-12-06 | 2002-01-16 | Affymetrix, Inc. (a Delaware Corporation) | Detection of nucleic acid sequences |
| US6004744A (en) | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
| RU1794088C (en) | 1991-03-18 | 1993-02-07 | Институт Молекулярной Биологии Ан@ Ссср | Method of dna nucleotide sequence determination and a device for its realization |
| US5324633A (en) | 1991-11-22 | 1994-06-28 | Affymax Technologies N.V. | Method and apparatus for measuring binding affinity |
| CA2130562A1 (en) | 1992-02-19 | 1993-09-02 | Alexander B. Chetverin | Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids |
| US5795714A (en) | 1992-11-06 | 1998-08-18 | Trustees Of Boston University | Method for replicating an array of nucleic acid probes |
| JP2004537292A (en) * | 2001-05-25 | 2004-12-16 | ディーエヌエープリント ジェノミクス インコーポレーティッド | Compositions and methods for estimating body color traits |
| FR2857979B1 (en) | 2003-07-25 | 2005-09-23 | Agronomique Inst Nat Rech | USE OF THE SILVER GENE FOR THE AUTHENTICATION OF THE RACIAL ORIGIN OF ANIMAL POPULATIONS AND THEIR DERIVED PRODUCTS |
-
2006
- 2006-08-16 DE DE06801706T patent/DE06801706T1/en active Pending
- 2006-08-16 CN CN200680036715.6A patent/CN101356285A/en active Pending
- 2006-08-16 WO PCT/US2006/032100 patent/WO2007022335A1/en active Application Filing
- 2006-08-16 AU AU2006279420A patent/AU2006279420B2/en active Active
- 2006-08-16 US US12/063,964 patent/US7807370B2/en active Active
- 2006-08-16 CA CA2619841A patent/CA2619841C/en active Active
- 2006-08-16 JP JP2008527129A patent/JP5203202B2/en active Active
- 2006-08-16 EP EP06801706.0A patent/EP1945803B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| AU2006279420B2 (en) | 2010-12-16 |
| WO2007022335A1 (en) | 2007-02-22 |
| DE06801706T1 (en) | 2009-07-30 |
| AU2006279420A1 (en) | 2007-02-22 |
| CA2619841C (en) | 2012-07-03 |
| JP5203202B2 (en) | 2013-06-05 |
| EP1945803A1 (en) | 2008-07-23 |
| EP1945803B1 (en) | 2014-07-02 |
| US7807370B2 (en) | 2010-10-05 |
| CN101356285A (en) | 2009-01-28 |
| JP2009504189A (en) | 2009-02-05 |
| US20080227102A1 (en) | 2008-09-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lyons et al. | Chocolate coated cats: TYRP1 mutations for brown color in domestic cats | |
| CN107815498B (en) | SNP molecular marker related to multiple economic traits of pig and application thereof | |
| CN112725462A (en) | SNP molecular marker combination related to body weight and body size of Longsheng chicken and screened based on whole genome sequencing and application | |
| Stadler et al. | Phylogenetic conservation and physical mapping of members of the H6 homeobox gene family | |
| Pacheco Contreras et al. | Convenient genotyping of nine bovine K-casein variants | |
| KR20170124003A (en) | Method for predicting skin pigmentation | |
| CN105441536A (en) | SNP markers for discriminating the sex in the olive flounder | |
| CN113502335A (en) | Molecular marker related to sheep growth traits and application thereof | |
| EA027258B1 (en) | Method fo predicting the pattern of locomotion in a horse and use of said method for selection of a horse for breeding | |
| CN107937557B (en) | SNP (single nucleotide polymorphism) site related to economic traits of pigs and application thereof | |
| KR101823372B1 (en) | SNP Makers for Identification of WooriHeukDon Porcine and Method for Identifying WooriHeukDon Porcine using the same | |
| CA2619841A1 (en) | Methods for identifying merle mutations in the silv gene | |
| CN106929570B (en) | Method for identifying bull variety by using cattle Y chromosome mononucleotide genetic marker | |
| US20060068402A1 (en) | Methylated promoters of colon cancer-specific expression-decreased genes and use thereof | |
| CN108396063B (en) | Application of CDH23 gene mutation in molecular diagnosis of pituitary adenoma | |
| CN102260734A (en) | Method for rapidly detecting goat Six6 gene single nucleotide polymorphism and application thereof | |
| CN107937559B (en) | SNP (single nucleotide polymorphism) site related to economic traits of pigs and application thereof | |
| CN117385061B (en) | Molecular marker related to Hu sheep growth traits and application thereof | |
| CN113151509B (en) | Molecular marker related to chicken serum alkaline phosphatase level, detection primer, kit and application | |
| Lee et al. | Genetic quality control of the rat strains at the national bio resource project-rat | |
| Hecht | Sequence analysis of Pmel17 as candidate gene for causing rat-tail syndrome in cattle | |
| Abdullah et al. | Detection of Melanocortin 1 Receptor (MC1R) polymorphism and its relation to plumage colors in local turkey | |
| EP0914469A1 (en) | Methods for identifying a mutation in a gene of interest | |
| CN114774552A (en) | Diagnosis marker and diagnosis reagent for nevoid basal cell carcinoma syndrome and application | |
| KR101929340B1 (en) | The primer set for determining Apis mellifera species using analysis of single nucleotide polymorphism or composition comprising thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |