CA2613015C - Pyrrolo [2, 3-b] pyridine derivatives as protein kinase inhibitors - Google Patents

Pyrrolo [2, 3-b] pyridine derivatives as protein kinase inhibitors Download PDF

Info

Publication number
CA2613015C
CA2613015C CA2613015A CA2613015A CA2613015C CA 2613015 C CA2613015 C CA 2613015C CA 2613015 A CA2613015 A CA 2613015A CA 2613015 A CA2613015 A CA 2613015A CA 2613015 C CA2613015 C CA 2613015C
Authority
CA
Canada
Prior art keywords
optionally substituted
hydrogen
fluoro
nhr
compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2613015A
Other languages
French (fr)
Other versions
CA2613015A1 (en
Inventor
Prabha N. Ibrahim
Dean R. Artis
Ryan Bremer
Gaston Habets
Shumeye Mamo
Marika Nespi
Chao Zhang
Jiazhong Zhang
Yong-Liang Zhu
Rebecca Zuckerman
Brian West
Yoshisa Suzuki
James Tsai
Klaus-Peter Hirth
Gideon Bollag
Wayne Spevak
Hanna Cho
Samuel J. Gillette
Guoxian Wu
Hongyao Zhu
Shenghua Shi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Plexxikon Inc
Original Assignee
Plexxikon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37033831&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2613015(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Plexxikon Inc filed Critical Plexxikon Inc
Publication of CA2613015A1 publication Critical patent/CA2613015A1/en
Application granted granted Critical
Publication of CA2613015C publication Critical patent/CA2613015C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
    • A61K31/415—1,2-Diazoles
    • A61K31/416—1,2-Diazoles condensed with carbocyclic ring systems, e.g. indazole
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/4353—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems
    • A61K31/437—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems the heterocyclic ring system containing a five-membered ring having nitrogen as a ring hetero atom, e.g. indolizine, beta-carboline
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/496—Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
    • A61K31/5375—1,4-Oxazines, e.g. morpholine
    • A61K31/5377—1,4-Oxazines, e.g. morpholine not condensed and containing further heterocyclic rings, e.g. timolol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/06—Antiasthmatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/08—Drugs for disorders of the urinary system of the prostate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/06—Antipsoriatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/06—Antimigraine agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/16—Otologicals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/12—Drugs for disorders of the metabolism for electrolyte homeostasis
    • A61P3/14—Drugs for disorders of the metabolism for electrolyte homeostasis for calcium homeostasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/08—Antiallergic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
    • C07C37/62—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring by introduction of halogen; by substitution of halogen atoms by other halogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C39/00—Compounds having at least one hydroxy or O-metal group bound to a carbon atom of a six-membered aromatic ring
    • C07C39/24—Halogenated derivatives
    • C07C39/26—Halogenated derivatives monocyclic monohydroxylic containing halogen bound to ring carbon atoms
    • C07C39/27—Halogenated derivatives monocyclic monohydroxylic containing halogen bound to ring carbon atoms all halogen atoms being bound to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
    • C07C45/61—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups
    • C07C45/67—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups by isomerisation; by change of size of the carbon skeleton
    • C07C45/673—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups by isomerisation; by change of size of the carbon skeleton by change of size of the carbon skeleton
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
    • C07C45/61—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups
    • C07C45/67—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups by isomerisation; by change of size of the carbon skeleton
    • C07C45/68—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups by isomerisation; by change of size of the carbon skeleton by increase in the number of carbon atoms
    • C07C45/70—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups by isomerisation; by change of size of the carbon skeleton by increase in the number of carbon atoms by reaction with functional groups containing oxygen only in singly bound form
    • C07C45/71—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups by isomerisation; by change of size of the carbon skeleton by increase in the number of carbon atoms by reaction with functional groups containing oxygen only in singly bound form being hydroxy groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C47/00—Compounds having —CHO groups
    • C07C47/52—Compounds having —CHO groups bound to carbon atoms of six—membered aromatic rings
    • C07C47/56—Compounds having —CHO groups bound to carbon atoms of six—membered aromatic rings containing hydroxy groups
    • C07C47/565—Compounds having —CHO groups bound to carbon atoms of six—membered aromatic rings containing hydroxy groups all hydroxy groups bound to the ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C47/00—Compounds having —CHO groups
    • C07C47/52—Compounds having —CHO groups bound to carbon atoms of six—membered aromatic rings
    • C07C47/575—Compounds having —CHO groups bound to carbon atoms of six—membered aromatic rings containing ether groups, groups, groups, or groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/08—Indoles; Hydrogenated indoles with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, directly attached to carbon atoms of the hetero ring

Abstract

Compounds of formula III which are active on protein kinases are described, as well as methods of using such compounds to treat diseases and conditions associated with aberrant activity of protein kinases. (Formula III).

Description

DEMANDES OU BREVETS VOLUMINEUX
LA PRESENTE PARTIE DE CETTE DEMANDE OU CE BREVETS
COMPREND PLUS D'UN TOME.

NOTE: Pour les tomes additionels, veillez contacter le Bureau Canadien des Brevets.

JUMBO APPLICATIONS / PATENTS

THIS SECTION OF THE APPLICATION / PATENT CONTAINS MORE
THAN ONE VOLUME.

THIS IS VOLUME OF

NOTE: For additional volumes please contact the Canadian Patent Office.

PYRROLO[2,3-B]PYRIDINE DERIVATIVES AS PROTEIN KINASE INHIBITORS

FIELD OF THE INVENTION
[0002] The present invention relates to kinases and compounds which modulate kinases, and uses therefor. Particular embodiments contemplate disease indications which are amenable to treatment by modulation of kinase activity by the compounds of the present invention.

BACKGROUND OF THE INVENTION
[0003] The information provided herein is intended solely to assist the understanding of the reader.
None of the information provided nor references cited is admitted to be prior art to the present invention.
[0004] Receptor protein kinases regulate key signal transduction cascades that control or are involved in the control of a plethora of physiological functions including cellular growth and proliferation, cell differentiation, cellular development, cell division, cell adhesion, stress response, short-range contact-mediated axonal guidance, transcription regulation, aberrant mitogenesis, angiogenesis, abnormal endothelial cell-cell or cell-matrix interactions during vascular development, inflammation, lymphohematopoietic stem cell activity, protective immunity against specific bacteria, allergic asthma, aberrant tissue-specific responses to the activation of the JNK signal transduction pathway, cell transformation, memory, apoptosis, competitive activity-dependent synapse modification at the neuromuscular synapse, immunological mediation of disease, and calcium regulation.
[0005) Specific disease states associated with aberrant regulation of protein kinases include, for example without limitation, acrocephalo-syndactyly type I, acute myeloid leukemia, AIDS-induced non-Hodgkin's lymphoma, Alzheimer's disease, amyotrophic lateral sclerosis, arthritis, asthma, atherosclerosis, atopic dermatitis, autoimmune diseases, bacterial infection, bladder cancer, cancer of the breast, cancer of the central nervous system, cancer of the colon, cancer of the endometrium, cancer of the fallopian tube, cancer of the gastrointestinal tract, cancer of the ovary, heart failure, chronic myeloid leukemia, colon carcinoma, colorectal cancer, chronic obstructive pulmonary disease (COPD), Crouzon Syndrome, diabetes, diabetic nephropathy, emphysema, endometriosis, epidermoid cancer, fibrotic disorders, gastrointestinal stromal tumor (GIST), glomerulonephritis, Graves' disease, head injury, hepatocellular carcinoma, Hirschsprung's disease, human gliomas, immunodeficiency diseases, inflammatory disorders, ischemic stroke, Jackson-Weiss syndrome, leiomyosarcoma, leukemias, lupus nephritis, malignant melanoma, malignant nephrosclerosis, mastocytosis, mast cell tumors, melanoma of the colon, MEN2 syndromes, metabolic disorders, migraine, multiple sclerosis, myeloproliferative disorders, nephritis, neurodegenerative diseases, neurotraumatic diseases, non small cell lung cancer, organ transplant rejection, osteoporosis, pain, Parkinson's disease, Pfeiffer Syndrome, polycystic kidney disease, primary lymphoedema, prostate cancer, psoriasis, vascular restenosis, rheumatoid arthritis, dermal and tissue scarring, selective T-cell defect (STD), severe combined immunodeficiency (SCID), small cell lung cancer, spinal cord injury, squamous cell carcinoma, systemic lupus erythematosis, testicular cancer, thrombotic microangiopathy syndromes, Wegener's granulomatosis, X-linked agammaglobulinemia, viral infection, diabetic retinopathy, alopecia, erectile dysfunction, macular degeneration, chronic lymphocytic leukemia (CLL), myelodysplastic syndrome (MDS), neurofibromatosis, and tuberous sclerosis.
Accordingly, there is a need in the art for additional compounds and methods of use thereof for the modulation of receptor protein kinases.

[00061 This application is related to the following published patent applications: WO 2004024895, US 20040142864, WO 2004078923, US 20050170431, WO 2005028624, US 20050164300, and WO
2005062795.

SUMMARY OF THE INVENTION
[00071 The present invention concerns compounds active on protein kinases in general, including, but not limited to, Abl, Aktl, Akt2, Akt3, ALK, Alk5, B-Raf, Brk, Btk, Cdk2, CDK4, CDK5, CDK6, CHKI, c-Raf-1, Csk, EGFR, EphAl, EphA2, EphB2, EphB4, Erk2, Fak, FGFR1, FGFR2, FGFR3, FGFR4, Flt 1, Flt3, Flt4, Fins, Frk, Fyn, Gsk3a, Gsk3I , HCK, Her2/Erbb2, Her4/Erbb4, IGF1R, IKK beta, Irak4, Itk, Jakl, Jak2, Jak3, Jnkl, Jnk2, Jnk3, Kdr, Kit, LCK, MAP2K1, MAP2K2, MAP4K4, MAPKAPK2, Met, Mnkl, MLK1, p38, PDGFRA, PDGFRB, PDPK1, Piml, Pim2, Pim3, PKC alpha, PKC beta, PKC theta, Plkl, Pyk2, Ret, ROCK1, ROCK2, Ron, Src, Stk6, Syk, TEC, Tie2, TrkA, Yes, and/or Zap70, including any mutations of these kinases, and the use thereof in treating disease and conditions associated with regulation of the activity of the kinase. In particular, the invention concerns compounds of Formula I as described below. Thus, the invention provides novel use of compounds for therapeutic methods involving modulation of protein kinases, as well as novel compounds that can be used for therapeutic methods involving modulation of protein kinases.

[0008] The compounds of Formula I have the following structure:

Formula I
all salts, prodrugs, tautomers, and isomers thereof, wherein:
R2, R4, R5, and R6 are independently selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -CN, -NO2, -CRaRbR26, and -LR26;

R3 is selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -CN, -NO2, -CRaRbR26, -LR26 and -A-Ar-L1-R24;
A is selected from the group consisting of -0-, -S-, -CRaRb-, -NR'-, -C(O)-, -C(S)-, -S(O)-, and -S(O)2-;
R' is selected from the group consisting of hydrogen, lower alkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, -C(O)R7, -C(S)R', -S(O)2R', -C(O)NHR', -C(S)NHR', and -S(O)2NHR7, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, lower alkylthio, mono-alkylamino, di-alkylamino, and -NR8R9, wherein the alkyl chain(s) of lower alkoxy, lower alkylthio, mono-alkylamino, or di-alkylamino are optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl chain carbon bound to 0 of alkoxy, S of thioalkyl or N of mono- or di-alkylamino is fluoro, further provided, however, that when R1 is lower alkyl, any substitution on the lower alkyl carbon bound to the N of -NR'- is fluoro, and wherein cycloalkyl, heterocycloalkyl, aryl or heteroaryl are optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;

R7 is selected from the group consisting of lower alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, lower alklylthio, mono-alkylamino, di-alkylamino, and NR$R9, provided, however, that any substitution of the alkyl carbon bound to the N of -C(O)NHR', -C(S)NHR7 or -S(O)2NHR7 is fluoro, wherein the alkyl chain(s) of lower alkoxy, lower alkylthio, mono-alkylamino, or di-alkylamino are optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl chain carbon bound to 0 of alkoxy, S of thioalkyl or N of mono- or di-alkylamino is fluoro, and wherein cycloalkyl, heterocycloalkyl, aryl and heteroaryl are optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
Ar is selected from the group consisting of optionally substituted arylene and optionally substituted heteroarylene;
L at each occurrence is independently selected from the group consisting of -(alk)a-S-(alk)b-, -(alk)a-O-(alk)b-, -(alk)a-NR25-(alk)b-, -(alk)a-C(O)-(alk)b-, -(alk)a-C(S)-(alk)b-, -(alk)a S(O)-(alk)b-, -(alk)a S(O)2-(alk)b-, -(alk)a-OC(O)-(alk)b-, -(alk)a C(O)O-(alk)b-, -(alk)a-OC(S)-(alk)b-, -(alk)a-C(S)O-(alk)b-, -(alk)a-C(O)NR25-(alk)b-, -(alk)a C(S)NR25-(alk)b-, -(alk)a S(O)2NR25-(alk)b-, -(alk)a NR25C(O)-(alk)b-, -(alk)a NR25C(S)-(alk)b-, -(alk)a NR25S(O)2-(alk)b-, -(alk)a NR25C(O)O-(alk)b-, -(alk)a-NR25C(S)O-(alk)b-, -(alk)a-OC(O)NR25-(alk)b-, -(alk)a-OC(S)NR25-(alk)b-, (alk)a NR25C(O)NR25-(alk)b-, -(alk)a NR25C(S)NR25-(alk)b-, and -(alk)a-NR25 S (O)2NR25 -(alk)b-;
a and b are independently 0 or 1;
alk is CI-3 alkylene or C1_3 alkylene substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkyl, lower alkoxy, lower alkylthio, mono-alkylamino, di-alkylamino, and -NRSR9, wherein lower alkyl or the alkyl chain(s) of lower alkoxy, lower alkylthio, mono-alkylamino or di-alkylamino are optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution of the alkyl chain carbon bound to 0 of alkoxy, S of thioalkyl or N of mono- or di-alkylamino is fluoro;
L1 is -(CRaR)õ or L, wherein v is 1, 2, or 3;

wherein Ra and Rb at each occurrence are independently selected from the group consisting of hydrogen, fluoro, -OH, -NH2, lower alkyl, lower alkoxy, lower alklylthio, mono-alkylamino, di-alkylamino, and -NR$R9, wherein the alkyl chain(s) of lower alkyl, lower alkoxy, lower alkylthio, mono-alkylamino, or di-alkylamino are optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl chain carbon bound to 0 of alkoxy, S of thioalkyl or N of mono- or di-alkylamino is fluoro; or any two of Ra and Rb on the same or different carbons combine to form a 3-7 membered monocyclic cycloalkyl or 5-7 membered monocyclic heterocycloalkyl and any others of Ra and Rb are independently selected from the group consisting of hydrogen, fluoro, -OH, -NH2, lower alkyl, lower alkoxy, lower alklylthio, mono-alkylamino, di-alkylamino, and -NR$R9, wherein the alkyl chain(s) of lower alkyl, lower alkoxy, lower alkylthio, mono-alkylamino, or di-alkylamino are optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl chain carbon bound to O of alkoxy, S of thioalkyl or N of mono- or di-alkylamino is fluoro, and wherein the 3-7 membered monocyclic cycloalkyl or 5-7 membered monocyclic heterocycloalkyl are optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
R8 and R9 combine with the nitrogen to which they are attached to form a 5-7 membered heterocycloalkyl optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, and fluoro substituted lower alkylthio;
R25 at each occurrence is independently selected from the group consisting of hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl; and R24 and R26 at each occurrence are independently selected from the group consisting of hydrogen, provided, however, that hydrogen is not bound to any of S(O), S(O)2, C(O) or C(S) of L or L,, optionally substituted lower alkyl, optionally substituted lower alkenyl, provided, however, that when R24 or R26 is optionally substituted lower alkenyl, no alkene carbon thereof is bound to N, S, 0, S(O), S(O)2, C(O) or C(S) of L or L1, optionally substituted lower alkynyl, provided, however, that when R24 or R26 is optionally substituted lower alkynyl, no alkyne carbon thereof is bound to N, S, 0, S(O), S(O)2, C(O) or C(S) of L or LI, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl.

[0009] The description above of substituents in Formula I includes descriptions of each combination of the specified substituents, R2, R3, R4, R5, and R. In some embodiments, at least one of R2, R3, R4, R5 and R6 is other than hydrogen.

[0010] In some embodiments involving compounds of Formula I, R2 and R6 are hydrogen, or R2 and R5 are hydrogen, or R2 and R4 are hydrogen, or R2 and R3 are hydrogen, or R3 and R6 are hydrogen, or R3 and R5 are hydrogen, or R3 and R4 are hydrogen, or R4 and R6 are hydrogen, or R4 and R5 are hydrogen, or R5 and R6 are hydrogen, wherein the substitutions at the other positions are non-hydrogen. In some embodiments, R2, R3 and R4 are hydrogen, or R2, R3 and R5 are hydrogen, or R2, R3 and R6 are hydrogen, or R2, R4 and R5 are hydrogen, or R2, R4 and R6 are hydrogen, or R2, R5 and R6 are hydrogen, or R3, R4 and R5 are hydrogen, or R3, R4 and R6 are hydrogen, or R3, R5 and R6 are hydrogen, or R4, R5 and R6 are hydrogen, wherein the substitutions at the other positions are non-hydrogen. In some embodiments, the compounds are mono-substituted with non-hydrogen at one of R2, R3, R4, R5 or R6 (i.e. hydrogen at the other four positions) . In some embodiments, compounds of Formula I have non-hydrogen substitution at R3; non-hydrogen substitution at R4; non-hydrogen substitution at R5; non-hydrogen substitution at R3 and R4; non-hydrogen substitution at R3 and R5. In some embodiments, the substitutions as listed are the only substitutions; the substitutions as listed are combined with R2 and R6 as H; the substitutions as listed are combined with substitution at one other of the substitution positions shown in Formula I. The compounds of Formula I, and all sub-embodiments detailed herein, may be used to treat a subject suffering from or at risk for any of the protein kinase mediated diseases or conditions contemplated herein.

[0011] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula la:

N
N H
Formula la all salts, prodrugs, tautomers, and isomers thereof, wherein Y3 is a bond, -CRaRb-, -A-Ar-LI-, or L, and Y5 is a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y3 or Y5 is a bond, or R26, provided, however, that neither of Y3R27 and Y5R27 are hydrogen, wherein Ra, Rb, L, LI, A, Ar and R26 are as defined with reference to Formula I.

[0012] In some embodiments of compounds of Formula Ia, Y3 and Y5 are bonds. In some embodiments, Y3 and Y5 are independently -CRaRb- or L. In some embodiments, Y3 and Y5 are independently L. In some embodiments, Y3 and Y5 are independently -CRaRb-. In some embodiments, Y3 is a bond, and Y5 is -CRaRb- or L. In some embodiments, Y3 is a bond, and Y5 is L.
In some embodiments, Y3 is a bond, and Y5 is -CRaRb-. In some embodiments, Y5 is a bond, and Y3 is -CRaRb- or L. In some embodiments, Y5 is a bond, and Y3 is L. In some embodiments, Y5 is a bond, and Y3 is -CRaRb-.

[0013] In some embodiments of any of the above embodiments of compounds of Formula la, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y3 or Y5 is a bond. In some embodiments, Y5 is a bond, -CRaRb-, or L, Y3 is -CRaRb-, -0-, -5-, -NR25-, -C(O)-, -C(S)-, -S(O)-, or -S(0)2-, wherein R25 is as defined for Formula I, and each R27 is independently R26 or Y5R27 is halogen; in further embodiments, Y3 is -CRaRb-or -C(O)-; in further embodiments, -CRaRb- is -CH2-. In some embodiments, Y5 is a bond, -CRaRb-, or L, R27 bound to Y5 is R26 or Y5R27 is halogen, Y3 is -CRaRb- or -C(O)-, and R27 bound to Y3 is optionally substituted aryl or optionally substituted heteroaryl.

[0014] In some embodiments of any of the above embodiments of compounds of Formula Ia, Y3R27 is -A-Ar-LI-R24, wherein A, Ar, and LI are as defined for Formula I, and R24 is substituted methyl, optionally substituted C2_6 alkyl, optionally substituted lower alkenyl, provided, however, that when R24 is optionally substituted lower alkenyl, no alkene carbon thereof is bound to N, S, 0, S(O), S(0)2, C(O) or C(S) of LI, optionally substituted lower alkynyl, provided, however, that when R24 is optionally substituted lower alkynyl, no alkyne carbon thereof is bound to N.
S, 0, S(O), S(0)2, C(O) or C(S) of LI, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, provided, however, that -A-Ar-LI-R24 is not --0 , wherein indicates the point of attachment to the 3 position of the azaindole ring; in further embodiments, R24 is optionally substituted C2.6 alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, or substituted methyl, wherein methyl is substituted with optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, when Ar is optionally substituted heteroarylene, the heteroarylene Q-N
ring is not a five or six membered ring having the structure Q Q 0-1 wherein indicates the point of attachment to A and - indicates the point of attachment to L1, and wherein the indicated N is either =N- or N=, and wherein each Q is independently a heteroaryl ring atom that may be optionally substituted. The term "heteroaryl ring atom"
refers to any atom that can be part of a heteroaryl ring structure (i.e., C, N, 0, or S).

[0015] In some embodiments of any of the above embodiments of compounds of Formula la, Y3 and Y5 are independently -0-, -S-, -CRaRb-, -NR25-, -C(O)-, -C(S)-, -S(O)-, or -S(O)2-, where Ra, Rb and R25 are as defined in Formula I, and each R27 is independently optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, Y5 is -0-, -NR25-, or -S(0)2-, preferably wherein R25 is hydrogen or lower alkyl, and R27 bound to Y3 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, preferably optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, Y3 is -CRaRb-, or -C(O)-, preferably -CH2- or -C(O)-, Y5 is -0-, -NR25-, or -S(0)2-, preferably -NR25-, wherein R25 is hydrogen or lower alkyl, and each R27 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, preferably optionally substituted aryl, or optionally substituted heteroaryl, provided, however, that Y3R27 is not OH, wherein indicates the bond to the 3 position H
N
of the 7-azaindole ring, and Y5R27 is not wherein indicates the bond to the 5 position of the 7-azaindole ring, i.e. the compound is not (3-hydroxy-phenyl)-(5-phenylamino-lH-pyrrolo[2,3-b]pyridin-3-yl)-methanone; in further embodiments, when R27 bound to Y3 is optionally substituted heteroaryl, the heteroaryl ring is not a five or six membered ring having the structure Q-N
- \ R200 Q Q 0-1 wherein indicates the point of attachment to Y3, and wherein the indicated N is either =N- or N=, and wherein each Q is independently a heteroaryl ring atom that may be appropriately optionally substituted and wherein R200 is other than hydrogen.
The term "other than hydrogen" and like terms refer to substituents contemplated herein which are not hydrogen. For example without limitation, if substituent ReX were defined as selected from the group consisting of hydrogen and optionally substituted lower alkyl, then the phrase "Re" is other than hydrogen" would contemplate only optionally substituted lower alkyl, i.e., all options of the substituent, excluding hydrogen.

[0016] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Ib:

N
N H
Formula lb all salts, prodrugs, tautomers, and isomers thereof, wherein Y3 and Y4 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y3 or Y4 is a bond, or R26, provided, however, that neither of Y3R27 and Y4R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0017] In some embodiments of compounds of Formula Ib, Y3 and Y4 are bonds. In some embodiments, Y3 and Y4 are independently -CRaRb- or L. In some embodiments, Y3 and Y4 are independently L. In some embodiments, Y3 and Y4 are independently -CRaRb-. In some embodiments, Y3 is a bond and Y4 is -CRaRb- or L. In some embodiments, Y3 is a bond and Y4 is L.
In some embodiments, Y3 is a bond and Y4 is -CRaRb-. In some embodiments, Y4 is a bond, and Y3 is -CRaRb- or L. In some embodiments, Y4 is a bond and Y3 is L. In some embodiments, Y4 is a bond and Y3 is -CRaRb-.

[0018] In some embodiments of any of the above embodiments of compounds of Formula Ib, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y3 or Y4 is a bond. In some embodiments, Y4 is a bond, -CRaRb-, or L, Y3 is -CRaRb-, -0-, -5-, -NR25-, -C(O)-, -C(S)-, -S(O)-, or -S(O)2-, wherein R25 is as defined for Formula I, and R27 is independently R26 or Y4R27 is halogen; in further embodiments, Y3 is -CRaRb- or -C(O)-; in further embodiments, -CRaRb- is -CH2-. In some embodiments, Y4 is a bond, -CRaRb-, or L, R27 bound to Y4 is R26 or Y4R27 is halogen, Y3 is -CRaRb- or -C(O)-, and R27 bound to Y3 is optionally substituted aryl or optionally substituted heteroaryl.

[0019] In some embodiments of any of the above embodiments of compounds of Formula Ib, Y3 and Y4 are independently -0-, -S-, -CRaRb-, -NR25-, -C(O)-, -C(S)-, -S(O)-, or -S(O)2-, where Ra, Rb and R25 are as defined in Formula I, and each R27 is independently optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl; in a further embodiment Y4 is -0-, -NR25-, or -S(0)2-, preferably wherein R25 is hydrogen or lower alkyl, and R27 bound to Y3 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, preferably optionally substituted aryl, or optionally substituted heteroaryl; in a further embodiment Y3 is -CRaRb-, or -C(O)-, preferably -CH2- or -C(O)-, Y4 is -0-, -NR 2'-, or -S(0)2-, preferably -NR25-, wherein R25 is hydrogen or lower alkyl, and each R27 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, preferably optionally substituted aryl, or optionally substituted heteroaryl.
[0020] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Ic:

R N
Formula Ic all salts, prodrugs, tautomers, and isomers thereof, wherein Y3 and Y6 are independently a bond, -CRaRb- , or L, and each R27 is independently halogen, provided that Y3 or Y6 is a bond, or R26, provided, however, that neither of Y3R27 and Y6R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0021] In some embodiments of compounds of Formula Ic, Y3 and Y6 are bonds. In some embodiments, Y3 and Y6 are independently -CRaRb- or L. In some embodiments, Y3 and Y6 are independently L. In some embodiments, Y3 and Y6 are independently -CRaRb-. In some embodiments, Y3 is a bond, and Y6 is -CRaRb- or L. In some embodiments, Y3 is a bond, and Y6 is L.
In some embodiments, Y3 is a bond, and Y6 is -CRaRb-. In some embodiments, Y6 is a bond, and Y3 is -CRaRb- or L. In some embodiments, Y6 is a bond, and Y3 is L. In some embodiments, Y6 is a bond, and Y3 is -CRaRb-.

[0022] In some embodiments of any of the above embodiments of compounds of Formula Ic, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y3 or y6 is a bond.

[0023] In some embodiments of any of the above embodiments of compounds of Formula Ia, Y3 and Y6 are independently -0-, -S-, -CRaRb-, -NR25-, -C(O)-, -C(S)-, -S(O)-, or -S(O)2-, where Ra, Rb and R25 are as defined in Formula I, and each R27 is independently optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, Y6 is -0-, -NR25-, or -S(O)2-, preferably wherein R25 is hydrogen or lower alkyl, and R27 bound to Y3 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, preferably optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, Y3 is -CRaRb-, or -C(O)-, preferably -CH2- or -C(O)-, Y6 is -0-, -NR 2'-, or -S(0)2-, preferably NR25-, wherein R25 is hydrogen or lower alkyl, and each R27 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, preferably optionally substituted aryl, or optionally substituted heteroaryl.
[0024] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Id:

N
N H
Formula Id all salts, prodrugs, tautomers, and isomers thereof, wherein Y3 and Y2 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y3 or Y2 is a bond, or R26, provided, however, that neither of Y2R27 and Y3R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0025] In some embodiments of compounds of Formula Id, Y3 and Y2 are bonds. In some embodiments, Y3 and Y2 are independently -CRaRb- or L. In some embodiments, Y3 and Y2 are independently L. In some embodiments, Y3 and Y2 are independently -CRaRb-. In some embodiments, Y3 is a bond, and Y2 is -CRaRb- or L. In some embodiments, Y3 is a bond, and Y2 is L.
In some embodiments, Y3 is a bond, and Y2 is -CRaRb-. In some embodiments, Y2 is a bond, and Y3 is -CRaRb- or L. In some embodiments, Y2 is a bond, and Y3 is L. In some embodiments, Y2 is a bond, and Y3 is -CRaRb-.

[0026] In some embodiments of any of the above embodiments of compounds of Formula Id, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y3 or Y2 is a bond.

[0027] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula le:

N
N H
Formula le all salts, prodrugs, tautomers, and isomers thereof, wherein Y4 and Y2 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y4 or Y2 is a bond, or R26, provided, however, that neither of Y2R27 and Y4R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0028] In some embodiments of compounds of Formula Ie, Y4 and Y2 are bonds. In some embodiments, Y4 and Y2 are independently -CRaRb- or L. In some embodiments, Y4 and Y2 are independently L. In some embodiments, Y4 and Y2 are independently -CRaRb-. In some embodiments, Y4 is a bond, and Y2 is -CRaRb- or L. In some embodiments, Y4 is a bond, and Y2 is L.
In some embodiments, Y4 is a bond, and Y2 is -CRaRb-. In some embodiments, Y2 is a bond and Y4 is -CRaRb- or L. In some embodiments, Y2 is a bond, and Y4 is L. In some embodiments, Y2 is a bond, and Y4 is -CRaRb-.

[0029] In some embodiments of any of the above embodiments of compounds of Formula Id, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y4 or Y2 is a bond.

[0030] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula If:

N H

Formula If all salts, prodrugs, tautomers, and isomers thereof, wherein Y5 and Y2 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y5 or Y2 is a bond, or R26, provided, however, that neither of Y2R27 and Y5R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0031] In some embodiments of compounds of Formula If, Y5 and Y2 are bonds. In some embodiments, Y5 and Y2 are independently -CRaRb- or L. In some embodiments, Y5 and Y2 are independently L. In some embodiments, Y5 and Y2 are independently -CRaRb-. In some embodiments, Y5 is a bond, and Y2 is -CRaRb- or L. In some embodiments, Y5 is a bond, and Y2 is L.
In some embodiments, Y5 is a bond, and Y2 is -CRRb-. In some embodiments, Y2 is a bond, and Y5 is -CRaRb- or L. In some embodiments, Y2 is a bond, and Y5 is L. In some embodiments, Y2 is a bond, and Y5 is -CRaRb-.

[0032] In some embodiments of any of the above embodiments of compounds of Formula If, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y5 or Y2 is a bond.

[0033] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Ig:

H
Formula Ig all salts, prodrugs, tautomers, and isomers thereof, wherein Y6 and Y2 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y6 or Y2 is a bond, or R26, provided, however, that neither of Y2R27 and Y6R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0034] In some embodiments of compounds of Formula Ig, Y6 and Y2 are bonds. In some embodiments, Y6 and Y2 are independently -CRaRb- or L. In some embodiments, Y6 and Y2 are independently L. In some embodiments, Y6 and Y2 are independently -CRaRb-. In some embodiments, Y6 is a bond, and Y2 is -CRaRb- or L. In some embodiments, Y6 is a bond, and Y2 is L.
In some embodiments, Y6 is a bond, and Y2 is -CRaRb-. In some embodiments, Y2 is a bond, and Y6 is -CRaRb- or L. In some embodiments, Y2 is a bond, and Y6 is L. In some embodiments, Y2 is a bond, and Y6 is -CRaRb-.

[0035] In some embodiments of any of the above embodiments of compounds of Formula Ig, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y6 or Y2 is a bond.

[0036] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Ih:

H
Formula Ih all salts, prodrugs, tautomers, and isomers thereof, wherein Y6 and Y4 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y6 or Y4 is a bond, or R26, provided, however, that neither of Y4R27 and Y6R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0037] In some embodiments of compounds of Formula Ih, Y6 and Y4 are bonds. In some embodiments, Y6 and Y4 are independently -CRaRb- or L. In some embodiments, Y6 and Y4 are independently L. In some embodiments, Y6 and Y4 are independently -CRaRb-. In some embodiments, Y6 is a bond, and Y4 is -CRaRb- or L. In some embodiments, Y6 is a bond, and Y4 is L.
In some embodiments, Y6 is a bond, and Y4 is -CRaRb-. In some embodiments, Y4 is a bond and Y6 is -CRaRb- or L. In some embodiments, Y4 is a bond, and Y6 is L. In some embodiments, Y4 is a bond, and Y6 is -CRaRb-.

[0038] In some embodiments of any of the above embodiments of compounds of Formula Ih, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y6 or Y4 is a bond.

[0039] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Ii:

:::6 N NH
Formula Ii all salts, prodrugs, tautomers, and isomers thereof, wherein Y6 and Y5 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y6 or Y5 is a bond, or R26, provided, however, that neither of Y5R27 and Y6R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0040] In some embodiments of compounds of Formula Ii, Y6 and Y5 are bonds. In some embodiments, Y6 and Y5 are independently -CRaRb- or L. In some embodiments, Y6 and Y5 are independently L. In some embodiments, Y6 and Y5 are independently -CRaRb-. In some embodiments, Y6 is a bond, and Y5 is -CRaRb- or L. In some embodiments, Y6 is a bond, and Y5 is L.
In some embodiments, Y6 is a bond, and Y5 is -CRaRb-. In some embodiments, Y5 is a bond, and Y6 is -CRaRb- or L. In some embodiments, Y5 is a bond, and Y6 is L. In some embodiments, Y5 is a bond, and Y6 is -CRaRb-.

[0041] In some embodiments of any of the above embodiments of compounds of Formula Ii, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y6 or Y5 is a bond.

[0042] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Ij:

N H
Formula Ij all salts, prodrugs, tautomers, and isomers thereof, wherein Y4 and Y5 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y4 or Y5 is a bond, or R26, provided, however, that neither of Y4R27 and Y5R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0043] In some embodiments of compounds of Formula Ij, Y4 and Y5 are bonds. In some embodiments, Y4 and Y5 are independently -CRaRb- or L. In some embodiments, Y4 and Y5 are independently L. In some embodiments, Y4 and Y5 are independently -CRaRb-. In some embodiments, Y4 is a bond, and Y5 is -CRaRb- or L. In some embodiments, Y4 is a bond, and Y5 is L.
In some embodiments, Y4 is a bond, and Y5 is -CRaRb-. In some embodiments, Y5 is a bond, and Y4 is -CRaRb- or L. In some embodiments, Y5 is a bond, and Y4 is L. In some embodiments, Y5 is a bond, and Y4 is -CRaRb-.

[0044] In some embodiments of any of the above embodiments of compounds of Formula Ij, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y4 or Y5 is a bond.

[0045] In some embodiments, compounds of Formula I have the structure according to the following sub-generic structure Formula Ik:

Y

N N
H
Formula Ik all salts, prodrugs, tautomers, and isomers thereof, wherein R26 is as defined in Formula I, and Y is selected from the group consisting of a bond, -CRaRb- and L, where Ra, Rb and L are as defined with reference to Formula I, provided that YR26 is not hydrogen.

[0046] In some embodiments of compounds of Formula Ik, Y is -(alk)a S-(alk)b-, -(alk)a O-(alk)b-, -(alk)a-OC(O)-(alk)b-, -(alk)a-C(O)O-(alk)b-, -(alk)a-OC(S)-(alk)b-, -(alk)a-C(S)O-(alk)b-, -(alk)a C(O)-(alk)b-, -(alk)a C(S)-(alk)b-, -(alk)a-C(O)NR25-(alk)b-, -(alk)a-OC(O)NR25-(alk)b-, -(alk)a-OC(S)NR25-(alk)b-, -(alk)a-C(S)NRZ5-(alk)b-, -(alk)a S(O)-(alk)b-, -(alk)a S(O)2-(alk)b-, -(alk)a S(O)2NR25-(alk)b-, -(alk)a-NR25-(alk)b-, -(alk)a-NR25C(O)-(alk)b-, -(alk)a NR25C(S)-(alk)b-, -(alk)a-NR25C(O) 25-(alk)b-, -(alk)a-NR25C(S)NR25-(alk)b-, -(alk)a NR25C(O)O-(alk)b-, -(alk)a-NR25C(S)O-(alk)b-, -(alk)a-NR25S(O)2-(alk)b-, or -(alk)a-NR25S(O)2NR25-(alk)b-, wherein alk, a, b, and R25 are as defined for Formula I.

[0047] In some embodiments of compounds of Formula Ik, Y is -S-(alk)b-, -O-(alk)b-, -OC(O)-(alk)b-, -C(O)O-(alk)b-, -OC(S)-(alk)b-, -C(S)O-(alk)b-, -C(O)-(alk)b-, -C(S)-(alk)b-, -C(O)NR25-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR2S-(alk)b-, -C(S)NR25-(alk)b-, -S(O)-(alk)b-, -S(O)2-(alk)b-, S(O)2NR25-(alk)b-, NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR2SC(O)NR25-(alk)b-, -NR25C(S)NR25-(alk)b-, -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein alk, b and R25 are as defined for Formula I.
[0048] In some embodiments of compounds of Formula Ik, Y is -S-(alk)b-, -O-(alk)b-, -OC(O)-(alk)b-, -OC(S)-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25-(alk)b-, -S(O)-(alk)b-, -S(O)2-(alk)b-, S(O)2NR25-(alk)b-, -NR25-(alk)b-, NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR25C(O)NR25-(alk)b-, -NR25C(S)NR25-(alk)b-, -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein alk, b and R25 are as defined for Formula I.

[0049] In some embodiments of compounds of Formula 1k, R26 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, Y is -0-, -S-, -NR25-, -C(O)-, -C(S)-, -S(O)-, or -S(O)2- and R26 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, Y is -NR25-, preferably wherein R25 is hydrogen or lower alkyl, preferably wherein Y is -NH-; in further embodiments, R26 is optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, lower alkyl is substituted with optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, heteroaryl is monocyclic. In some embodiments, Y is -NH-; in further embodiments, R26 is substituted phenyl or optionally substituted heteroaryl, provided that heteroaryl is monocyclic.

[0050] In some embodiments of any of the above embodiments of compounds of Formula Ilk, YR26 is not optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -C 2CH2N(CH3)2, -C(O)H, -CH2N(CH3)2, -C(O)OCH3, O

D
CHZOH, -OH, -OCH3, NHCHZCH N
, =CHz, -NHCHs, -NHCH2CH3, -NH phenyl O

N 1-1 ~ N
N ~~,~ N
or , wherein indicates the bond attached to the 5-position of the 7-azaindole ring; and when Y is -0- or -NR25-, then R26 is not N

which is optionally substituted, wherein indicates the bond attached to Y; and N=\

-~ N

when Y is -0-, then R26 is not which is optionally substituted, wherein indicates the bond attached Y.

[0051] In some embodiments, compounds of Formula I have the structure according to the following sub-generic structure Formula Im:

N N
H

Formula Im all salts, prodrugs, tautomers, and isomers thereof, wherein R26 and Y are as defined for Formula Ik.
[0052] In some embodiments of compounds of Formula Im, Y is -OC(O)-(alk)b-, -C(O)O-(alk)b-, -OC(S)-(alk)b-, -C(S)O-(alk)b-, -C(O)-(alk)b-, -C(S)-(alk)b-, -C(O)NR25-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25-(alk)b-, -C(S)NR25-(alk)b-, -S(O)-(alk)b-, -S(O)2-(alk)b-, S(O)2NR25-(alk)b-, C(O)O-(alk)b-, -NR25C(S)-(alk)b-, -NR25C(O)NR25-(alk)b-, -NR25COS NR25-(alk)b-, NR 25 -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein alk, b and R25 are as defined for Formula I.

[0053] In other embodiments of compounds of Formula Im, Y is -S-(alk)b-, -O-(alk)b-, -OC(O)-(alk)b-, -C(O)O-(alk)b-, -OC(S)-(alk)b-, -C(S)O-(alk)b-, -C(O)-(alk)b-, -C(S)-(alk)b-, -C(O)NR25-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25-(alk)b-, -C(S)NR25-(alk)b-, -S(O)-(alk)b-, -S(O)2-(alk)b-, -S(O)2NR25-(alk)b-, -NR25-(alk)b-, NR25C(O)-(alk)b-, NR25C(S)-(alk)b-, -NR25C(O)NR2s_(alk)b-, -NR25C(S)NR25-(alk)b-, -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein alk, b and RZ5 are as defined for Formula I.
[0054] In other embodiments of compounds of Formula Im, Y is -S-(alk)b-, -O-(alk)b-, -OC(O)-(alk)b-, -OC(S)-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25-(alk)b-, -S(O)2-(alk)b-, -S(O)2NR25-(alk)b-, NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR25C(O)NR25_(alk)b-, -NR25C(S)NR25_(alk)b-, -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein alk, b and R25 are as defined for Formula I.

[0055] In some embodiments of compounds of Formula Im, R26 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, Y is -0-, -S-, -NR25-, -C(O)-, -C(S)-, -S(O)-, or -S(O)2- and R26 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, Y is -NR25-, preferably wherein R25 is hydrogen or lower alkyl, preferably wherein Y is -NH-; in further embodiments, R26 is optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, lower alkyl is substituted with optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, heteroaryl is monocyclic.

[0056] In some embodiments of any of the above embodiments of compounds of Formula Im, when Y is -CH2NH-, then R26 is not optionally substituted thiophene or optionally substituted pyridine;
when Y is -0- or -NH-, then R26 is not optionally substituted bicyclic heteroaryl; when Y is -0-, then R26 is not optionally substituted phenyl; when Y is -NH- or -N(CH3)- and R26 is substituted phenyl, the phenyl is not substituted by halogen ortho to Y and optionally substituted amine para to Y, Y is not -NH-C(O)- or -C(O)-NH-, and YR26 is not optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted -CH=CH2, -C(O)OH, -C(O)OCH3, -C(O)OtBu, -OH, -OCH3, -NHCH2CH=CH2, -N(CH3)2, -NH2, H CI
N CI
F / \ N ,,N N
~ , F `l NN

_~ 0 NH2 -NHCH2C(O)OCH2CH3, -N(CH3)phenyl, F N
O IaT NH2 NH2 or ~NHS(O)2~
'- wherein - indicates the bond attached to the 4-position of the 7-azaindole ring.

[0057] In some embodiments, compounds of Formula I have the structure according to the following sub-generic structure Formula In:

Formula In all salts, prodrugs, tautomers, and isomers thereof, wherein R26 and Y are as defined for Formula Ik.
[0058] In other embodiments of compounds of Formula In, Y is -S-(alk)b-, -O-(alk)b-, -OC(O)-(alk)b-, -C(O)O-(alk)b-, -OC(S)-(alk)b-, -C(S)O'(alk)b-, -C(O)-(alk)b-, -C(S)-(alk)b-, -C(O)NR25-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25-(alk)b-, -C(S)NR25-(alk)b-, -S(O)-(alk)b-, -S(O)2-(alk)b-, -S(O)2NR25-(alk)b-, -NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR25C(O)NR25-(alk)b-, -NR25C(S)NR25-(alk)b-, NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or NR25S(O)2NR25-(alk)b-, wherein alk, b and R25 are as defined for Formula I.
[0059] In other embodiments of compounds of Formula In, Y is -S-(alk)b-, -O-(alk)b-, -OC(O)-(alk)b-, -OC(S)-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25-(alk)b-, -S(O)-(alk)b-, -S(O)2-(alk)b-, -S(O)2NR25-(alk)b-, -NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR25C(O)NR.25-(alk)b-, -NR25C(S)NR25-(alk)b-, -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR2SS(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein alk, b and R25 are as defined for Formula I.

[0060] In some embodiments of compounds of Formula In, R26 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, Y is -NR25-, preferably wherein R25 is hydrogen or lower alkyl, preferably wherein Y is -NH-; in further embodiments, R26 is optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, lower alkyl is substituted with optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl.

[0061] In some embodiments of any of the above embodiments of compounds of Formula In, N=\
-~ ~ N

compounds are excluded where Y is -0- and R26 is optionally substituted wherein -N
N
indicates the bond attached to Y; and where YR26 is -NH2a -CH3, -OC(O)phenyl, H

H
N
_H I \ H N

or , wherein indicates the bond attached to the 6-position of the 7-azaindole ring.

[0062] In some embodiments, compounds of Formula I have the structure according to the following sub-generic structure Formula lo:

rl 3 N
N H
Formula Io all salts, prodrugs, tautomers, and isomers thereof, wherein R26 and Y are as defined for Formula Ik.
[0063] In other embodiments of compounds of Formula lo, Y is -S-(alk)b-, -O-(alk)b-, -OC(O)-(alk)b-, -C(O)O-(alk)b-, -OC(S)-(alk)b-, -C(S)O-(alk)b-, -C(O)-(alk)b-, -C(S)-(alk)b-, -C(O)NR25-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25 -(alk)b-, -C(S)NR25-(alk)b-, -S(O)-(alk)b-, -S(O)2-(alk)b-, -S(O)2NR25-(alk)b-, -NR25-(alk)b-, -NR25C(O)-(alk)b-, NR25C(S)-(alk)b-, -NR25C(O)NR25-(alk)b-, -NR25C(S)NR25-(alk)b-, -NR25C(O)O-(alk)b-, NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or NR25S(O)2NR25-(alk)b-, wherein alk, b and R25 are as defined for Formula I.
[0064] In other embodiments of compounds of Formula lo, Y is -S-(alk)b-, -O-(alk)b-, -OC(O)-(alk)b-, -OC(S)-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25-(alk)b-, -S(O)-(alk)b-, -S(O)2-(alk)b-, -S(O)2NR25-(alk)b-, -NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR2sC(S)-(alk)b-, -NR25C(O)NR25-(alk)b-, -NR25C(S)NR25-(alk)b-, -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein alk, b and R25 are as defined for Formula I.

[0065] In some embodiments of compounds of Formula lo, R26 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, Y is -NR25-, preferably wherein R25 is hydrogen or lower alkyl, preferably wherein Y is -NH-; in further embodiments, R26 is optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, lower alkyl is substituted with optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl.

[0066] In some embodiments of any of the above embodiments of compounds of Formula lo, compounds are excluded when -YR26 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -CH2NR'R", wherein NR'R" is optionally substituted heterocycloalkyl or optionally substituted heteroaryl, -C(O)NR'R", wherein NR'R" is optionally substituted heterocycloalkyl or optionally substituted heteroaryl, or R' is H and R" is optionally substituted cycloalkyl, optionally substituted aryl or optionally substituted heteroaryl, optionally substituted -CH=CH2, -CH3, -CH2CH2NHCH3, -CH2CH(NH2)C(O)OH, -CH2CH(NH2)C(O)OCH3, -CH2CH(C(O)OH)NHCH3i -C(O)C(O)OCH3a -CH2C(O)OCH2CH3i -CH2C(O)NH2, -CH2CN, -NH2, -N(CH3)2, -SCH3, -N=C(CH3)NHOAc, -C(O)CC13, -C(O)OCH3, -C(O)CH2Br, -C(O)NH2, -C(S)NH2, -CH2NH-thiophene, wherein thiophene is optionally substituted, n~/- 7 \N
H
r-N N H O N.
r N
wherein the phenyl ring is optionally substituted, O
iN N
N
\ S N ( N-O O +--. N/
O H H
a ~ o CI S _ õ -O
N C(O)NH(CH2)2SO2CH3 N)( N- ~S
H H Br H

NH N~~
~-~N/ \ S (CH2)3CH3 H O
N--NH F F
N O F F

, H2N 0 , O o 0 rr \ \ \ I \ F \ I \ OH
r N CDN

, , p I o \ \ \ O \ \ NHZ \ \ NOZ \
O/ I/ F

N o/0 `t OH t O
NN`N P

~ D~

H D3 wherein the ring is optionally substituted at D1, D, o D2 No N
;tn S
DZ and D3, D3 wherein the ring is optionally substituted at D1, D2 and D3, -o ,o N
O O o -0 ,0 H O
HNN \ HN-N'ZiNH N~
1,,, r 0 f-x/ -MD

O OH

N N / N
N N N N N
H H
\ (') OH

~ COOH NH
NH N TN N O

v~ ($'2.'HN o , 1 IY , N N ---( D o-i-S
)aF
HHN

0 0 +QF 0 O 0 CF
_S _S
- CI - -CF3 I/ O I/ O I/ p 11 o CI -o -o g S
I
CI O O N H NH
~\ N
S
O
NH
H ~ ~ R
when R is H, F, Cl, CH3, CF3 or OCH3, R
O
H
N when R is H, 4-F, 4-CF3 or 3-F, or O
H \ /R
N

R' when R' is OCH3 or CH3 and R is CF3, or when R' is CH3 and R
is Cl, wherein indicates the bond attached to the 3-position of the 7-azaindole ring.

[0067] In some embodiments, compounds of Formula I have the structure according to the following sub-generic structure Formula Ip:

N H

Formula Ip all salts, prodrugs, tautomers, and isomers thereof, wherein R26 and Y are as defined for Formula Ik.
[0068] In other embodiments of compounds of Formula Ip, Y is -S-(alk)b-, -O-(alk)b-, -OC(O)-(alk)b-, -C(O)O-(alk)b-, -OC(S)-(alk)b-, -C(S)O-(alk)b-, -C(O)-(alk)b-, -C(S)-(alk)b-, -C(O)NR25-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25-(alk)b-, -C(S)NR25-(alk)b-, -S(O)-(alk)b-, -S(O)2-(alk)b-, -S(O)2NR25-(alk)b-, -NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR25C(O)NR25-(alk)b-, NRZSC(S)NR25-(alk)b-, -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein alk, b and R25 are as defined for Formula I.
[0069] In other embodiments of compounds of Formula Ip, Y is -S-(alk)b-, -O-(alk)b-, -OC(O)-(alk)b-, -OC(S)-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25-(alk)b-, -S(O)-(alk)b-, -S(O)2-(alk)b-, -S(O)2NR25-(alk)b-, NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR25C(O)NR25-(alk)b-, -NR25C(S)NR25-(alk)b-, -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein alk, b and RZ5 are as defined for Formula I.

[0070] In some embodiments of compounds of Formula Ip, R26 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, Y is -NR25-, preferably wherein R25 is hydrogen or lower alkyl, preferably wherein Y is -NH-; in further embodiments, R26 is optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl; in further embodiments, lower alkyl is substituted with optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl.

[0071] In some embodiments of any of the above embodiments of compounds of Formula Ip above, compounds are excluded when YR26 is -CH3, optionally substituted aryl, optionally substituted "1 0 F
0 0 0 o 0' O 0 -"~ I o ';I o cycloalkyl, , , o~ '0 or c', wherein -~ indicates the bond attached to the 2-position of the 7-azaindole ring.

[0072] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Iq:

YsRz7 y2R27 N
N H
Formula Iq all salts, prodrugs, tautomers, and isomers thereof, wherein Y2 Y3 and Y4 are independently a bond, -CRaRt- or L, and each R27 is independently halogen, provided that Y2, Y3 or Y4 is a bond, or R26 provided, however, that none of Y2R27, Y3R27, and Y4R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0073] In some embodiments of compounds of Formula Iq, Y2, Y3 and Y4 are bonds. In some embodiments, Y2, Y3 and Y4 are independently -CRaRb- or L. In some embodiments, Y2, Y3 and Y4 are independently L. In some embodiments, one of Y2, Y3 and Y4 is a bond, and the others are independently -CRaRb- or L. In some embodiments, one of Y2, Y3 and Y4 is a bond, and the others are independently L. In some embodiments, two of Y2, Y3 and Y4 are bonds, and the other is -CRaRb- or L. In some embodiments, two of Y2, Y3 and Y4 are bonds and the other is L.

[0074] In some embodiments of any of the above embodiments of compounds of Formula Iq, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y2, Y3 or Y4 is a bond.

[0075] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Ir:

N H

Formula Ir all salts, prodrugs, tautomers, and isomers thereof, wherein Y2 Y3 and Y5 are independently a bond, -CRaRb- or L, and each R27 is independently halogen, provided that Y2, Y3 or Y5 is a bond, or Rz6, provided, however, that none of Y2R27, Y3R27, and Y5R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0076] In some embodiments of compounds of Formula Ir, Y2, Y3 and Y5 are bonds. In some embodiments, Y2, Y3 and Y5 are independently -CRaRb- or L. In some embodiments, Y2, Y3 and Y5 are independently L. In some embodiments, any one of Y2, Y3 and Y5 is a bond, and the remaining of Y2, Y3 and Y5 are independently -CRaRb- or L. In some embodiments, any one of Y2, Y3 and Y5 is a bond, and the remaining of Y2, Y3 and Y5 are independently L. In some embodiments, any two of Y2, Y3 and Y5 are bonds, and the remaining of Y2, Y3 and Y5 is -CRaRb- or L. In some embodiments, any two of Y2, Y3 and Y5 are bonds, and the remaining of Y2, Y3 and Y5 is L.

[0077] In some embodiments of any of the above embodiments of compounds of Formula Ir, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y2, Y3 or Y5 is a bond.

[0078] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Is:

Formula Is all salts, prodrugs, tautomers, and isomers thereof, wherein Y2 Y3 and Y6 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y2, Y3 or Y6 is a bond, or R26, provided, however, that none of Y2RZ7, Y3R27, and Y6R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0079] In some embodiments of compounds of Formula Is, Y2, Y3 and Y6 are bonds. In some embodiments, Y2, Y3 and Y6 are independently -CRaRb- or L. In some embodiments, Y2, Y3 and Y6 are independently L. In some embodiments, any one of Y2, Y3 and Y6 is a bond, and the remaining of Y2, Y3 and Y6 are independently -CRaRb- or L. In some embodiments, any one of Y2, Y3 and Y6 is a bond, and the remaining of Y2, Y3 and Y6 are independently L. In some embodiments, any two of Y2, Y3 and Y6 are bonds, and the remaining of Y2, Y3 and Y6 is -CRaRb- or L. In some embodiments, any two of Y2, Y3 and Y6 are bonds, and the remaining of Y2, Y3 and Y6 is L.

[0080] In some embodiments of any of the above embodiments of compounds of Formula Is, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y2, Y3 or Y6 is a bond.

[0081] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula It:

N
N H
Formula It all salts, prodrugs, tautomers, and isomers thereof, wherein Y2 Y4 and Y5 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y2, Y4 or Y5 is a bond, or R26, provided, however, that none of Y2R27, Y4R27, and Y5R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0082] In some embodiments of compounds of Formula It, Y2, Y4 and Y5 are bonds. In some embodiments, Y2, Y4 and Y5 are independently -CRaRb- or L. In some embodiments, Y2, Y4 and Y5 are independently L. In some embodiments, any one of Y2, Y4 and Y5 is a bond, and the remaining of Y2, Y4 and Y5 are independently -CRaRb- or L. In some embodiments, any one of Y2, Y4 and Y5 is a bond, and the remaining of Y2, Y4 and Y5 are independently L. In some embodiments, any two of Y2, Y4 and Y5 are bonds, and the remaining of Y2, Y4 and Y5 is -CRaRb- or L. In some embodiments, any two of Y2, Y4 and Y5 are bonds, and the remaining of Y2, Y4 and Y5 is L.

[00831 In some embodiments of any of the above embodiments of compounds of Formula It, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y2, Y4 or Y5 is a bond.

[0084] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula lu:

R27Ys N N
H
Formula Iu all salts, prodrugs, tautomers, and isomers thereof, wherein Y2 Y4 and Y6 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y2, Y4 or Y6 is a bond, or R26, provided, however, that none of Y2R27, Y4R27, and Y6R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0085] In some embodiments of compounds of Formula lu, Y2, Y4 and Y6 are bonds. In some embodiments, Y2, Y4 and Y6 are independently -CRaRb- or L. In some embodiments, Y2, Y4 and Y6 are independently L. In some embodiments, any one of Y2, Y4 and Y6 is a bond, and the remaining of Y2, Y4 and Y6 are independently -CRaRb- or L. In some embodiments, any one of Y2, Y4 and Y6 is a bond, and the remaining of Y2, Y4 and Y6 are independently L. In some embodiments, any two of Y2, Y4 and Y6 are bonds, and the remaining of Y2, Y4 and Y6 is -CRaRb- or L. In some embodiments, any two of Y2, Y4 and Y6 are bonds, and the remaining of Y2, Y4 and Y6 is L.

[0086] In some embodiments of any of the above embodiments of compounds of Formula lu, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y2, Y4 or Y6 is a bond.

[0087] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Iv:
::xri-Formula Iv all salts, prodrugs, tautomers, and isomers thereof, wherein Y2 Y5 and Y6 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y2, Y5 or Y6 is a bond, or R26, provided, however, that none of Y2R27, YSR27, and Y6R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0088] In some embodiments of compounds of Formula Iv, Y2, Y5 and Y6 are bonds. In some embodiments, Y2, Y5 and Y6 are independently -CRaRb- or L. In some embodiments, Y2, Y5 and Y6 are independently L. In some embodiments, any one of Y2, Y5 and Y6 is a bond, and the remaining of Y2, Y5 and Y6 are independently -CRaRb- or L. In some embodiments, any one of Y2, Y5 and Y6 is a bond, and the remaining of Y2, Y5 and Y6 are independently L. In some embodiments, any two of Y2, Y5 and Y6 are bonds, and the remaining of Y2, Y5 and Y6 is -CRaRb- or L. In some embodiments, any two of Y2, Y5 and Y6 are bonds, and the remaining of Y2, Y5 and Y6 is L.

[0089] In some embodiments of any of the above embodiments of compounds of Formula Iv, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y2, Y5 or Y6 is a bond.

[0090] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula 1w:

N
N H
Formula Iw all salts, prodrugs, tautomers, and isomers thereof, wherein Y3 Y4 and Y5 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y3, Y4 or Y5 is a bond, or R26, provided, however, that none of Y3R27, Y4R27, and Y5R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0091] In some embodiments of compounds of Formula 1w, Y3, Y4 and Y5 are bonds. In some embodiments, Y3, Y4 and Y5 are independently -CRaRb- or L. In some embodiments, Y3, Y4 and Y5 are independently L. In some embodiments, any one of Y3, Y4 and Y5 is a bond, and the remaining of Y3, Y4 and Y5 are independently -CRaRb- or L. In some embodiments, any one of Y3, Y4 and Y5 is a bond, and the remaining of Y3, Y4 and Y5 are independently L. In some embodiments, any two of Y3, Y4 and Y5 are bonds, and the remaining of Y3, Y4 and Y5 is -CRaRb- or L. In some embodiments, any two of Y3, Y4 and Y5 are bonds, and the remaining of Y3, Y4 and Y5 is L.

[0092] In some embodiments of any of the above embodiments of compounds of Formula 1w, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y3, Y4 or Y5 is a bond.

[0093] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Ix:
Y4R27 Y3Rz7 Formula Ix all salts, prodrugs, tautomers, and isomers thereof, wherein Y3 Y4 and Y6 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y3, Y4 or Y6 is a bond, or R26, provided, however, that none of Y3R27, Y4R27, and Y6R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0094] In some embodiments of compounds of Formula Ix, Y3, Y4 and Y6 are bonds. In some embodiments, Y3, Y4 and Y6 are independently -CRaRb- or L. In some embodiments, Y3, Y4 and Y6 are independently L. In some embodiments, any one of Y3, Y4 and Y6 is a bond, and the remaining of Y3, Y4 and Y6 are independently -CRaRb- or L. In some embodiments, any one of Y3, Y4 and Y6 is a bond, and the remaining of Y3, Y4 and Y6 are independently L. In some embodiments, any two of Y3, Y4 and Y6 are bonds, and the remaining of Y3, Y4 and Y6 is -CRRb- or L. In some embodiments, any two of Y3, Y4 and Y6 are bonds, and the remaining of Y3, Y4 and Y6 is L.

[0095] In some embodiments of any of the above embodiments of compounds of Formula Ix, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y3, Y4 or Y6 is a bond.

[0096] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula ly:

H
Formula ly all salts, prodrugs, tautomers, and isomers thereof, wherein Y3 Y5 and Y6 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y3, Y5 or Y6 is a bond, or R26, provided, however, that none of Y3R27, Y5R27, and Y6R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0097] In some embodiments of compounds of Formula ly, Y3, Y5 and Y6 are bonds. In some embodiments, Y3, Y5 and Y6 are independently -CRaRb- or L. In some embodiments, Y3, Y5 and Y6 are independently L. In some embodiments, any one of Y3, Y5 and Y6 is a bond, and the remaining of Y3, Y5 and Y6 are independently -CRaRb- or L. In some embodiments, any one of Y3, Y5 and Y6 is a bond, and the remaining of Y3, Y5 and Y6 are independently L. In some embodiments, any two of Y3, Y5 and Y6 are bonds, and the remaining of Y3, Y5 and Y6 is -CRaRb- or L. In some embodiments, any two of Y3, Y5 and Y6 are bonds, and the remaining of Y3, Y5 and Y6 is L.

[0098] In some embodiments of any of the above embodiments of compounds of Formula ly, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y3, Y5 or Y6 is a bond.

[0099] In some embodiments, the compound of Formula I has a structure according to the following sub-generic structure Formula Iz:

R Formula Iz all salts, prodrugs, tautomers, and isomers thereof, wherein Y4 Y5 and Y6 are independently a bond, -CRaRb-, or L, and each R27 is independently halogen, provided that Y4, Y5 or Y6 is a bond, or R26, provided, however, that none of Y4R27, Y5R27, and Y6R27 are hydrogen, wherein Ra, Rb, L and R26 are as defined with reference to Formula I.

[0100] In some embodiments of compounds of Formula Iz, Y4, Y5 and Y6 are bonds. In some embodiments, Y4, Y5 and Y6 are independently -CRaRb- or L. In some embodiments, Y4, Y5 and Y6 are independently L. In some embodiments, any one of Y4, Y5 and Y6 is a bond, and the remaining of Y4, Y5 and Y6 are independently -CRaRb- or L. In some embodiments, any one of Y4, Y5 and Y6 is a bond, and the remaining of Y4, Y5 and Y6 are independently L. In some embodiments, any two of Y4, Y5 and Y6 are bonds, and the remaining of Y4, Y5 and Y6 is -CRaRb- or L. In some embodiments, any two of Y4, Y5 and Y6 are bonds, and the remaining of Y4, Y5 and Y6 is L.

[0101] In some embodiments, of any of the above embodiments of compounds of Formula Iz, each R27 is independently optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R27 is halogen, provided that Y4, Y5 or Y6 is a bond.

[0102] The compounds of Formulae Ia-Iz, and all sub-embodiments detailed herein, may be used to treat a subject suffering from or at risk for any of the protein kinase mediated diseases or conditions contemplated herein.

[0103] In some embodiments, compounds of Formula I have the structure according to the following sub-generic structure Formula II:

A
:/R13 Formula II
all salts, prodrugs, tautomers and isomers thereof, wherein:
A, R4, R5 and R6 are as defined with reference to Formula I; and R12, R13, R'4, R'5 and R'6 are independently selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -CN, -NO2, -CRaRbR24, and -LR24, where L and R24 are as defined for Formula I.

[01041 In some embodiments of compounds of Formula II, at least one of R12, R13, R14, R15 and R16 is other than hydrogen. In some embodiments, at least two of R12, R13, R14, R15 and R16 are other than hydrogen. In some embodiments, at least three of R12, R13, R14, R15 and R16 are other than hydrogen.
In some embodiments, at least four of R12, R13, R14, R15 and R16 are other than hydrogen. In some embodiments, R12, R13, R14, R15 and R16 are other than hydrogen.

[0105] In some embodiments of compounds of Formula II, one of R12, R13, R14, R15 and R16 is other than hydrogen, and the others of R'2, R'3, R14, R15 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 is other than hydrogen and R13, R14, R15 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R13 is other than hydrogen, and R12, R14, R'5 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R14 is other than hydrogen, and R12, R13, R15 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio.
[01061 In some embodiments of compounds of Formula II, any two of R12, R13, R'4, R'5 and R16 are independently other than hydrogen, and the others of R12, R13, R14, R15 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 and R13 are each independently other than hydrogen, and R14, R15 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 and R14 are each independently other than hydrogen, and R'3, R15 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 and R15 are each independently other than hydrogen, and R13, R14 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 and R16 are each independently other than hydrogen, and R13, R14 and R15 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R13 and R14 are each independently other than hydrogen, and R12, R15 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R13 and R15 are each independently other than hydrogen, and R'2, R14 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio.

[0107] In some embodiments of any of the above embodiments of compounds of Formula II, wherein any of R12 R13 R14 R1s and R16 is designated as other than hydrogen, each such R'z R13 R14, R15, or R16 is independently -LR24.

[0108] In some embodiments of any of the above embodiments of compounds of Formula II, R5 is other than hydrogen, and R4 and R6 are hydrogen, or R4 is other than hydrogen, and R5 and R6 are hydrogen, or R6 is other than hydrogen, and R4 and R5 are hydrogen, or R4 and R5 are other than hydrogen, and R6 is hydrogen, or R4 and R6 are other than hydrogen, and R5 is hydrogen, or R5 and R6 are other than hydrogen, and R4 is hydrogen, or R4, R5 and R6 are other than hydrogen.

[0109] In some embodiments of compounds of Formula II, the following compounds are excluded:
R4, R5, R6, R12, R13, R14, R15 and R16 are hydrogen and A is -CH2-, -S- or -S(O)2-;

R4, R6, R12, R13, R14, R15 and R16 are hydrogen, R5 is -Br or thiophen-3-yl, and A is -C(O)-;

R4 is 3, 5 di-fluorophenyl, -NH2, or -NO2, R5, R6, R12, R13, R14, R15 and R16 are hydrogen, and A is -C(O)-;

R4 is NO2, R5 is Br, R12, R13, R14, R15 and R16 are hydrogen, and A is -C(O)-;

Rs is -Br, R4, R6, R12, R13, R14, R's and R16 are hydrogen, and A is -S(O)-;

R12 is -CH3 or -F, R6, R13, R14, R15 and R16 are hydrogen, and A is -C(O)-;

R12 is -OH, Ra R6, R13 R14 R15 and R16 are hydrogen, Rs is thio hen-2- 1 and A
is -C(O)-;
R12 is -CF3, Ra R5, R6, R13 R14, R's and R16 are hydrogen, and A is -S(O) R13 is -OH or -OCH3, R4, R6, R12, R14, R15 and R16 are hydrogen, and A is -CH2-;

R13 is -OCH3, Rs, R6, R12, R14, R15 and R16 are hydrogen, Ra is -Br, and A is -CH2-;

R13 is -OH or -OCH3, Ra R6, R12 R14 R15 and R16 are hydrogen, Rs is thiophen-2-yl, and A is -CHOH-;

R13 is o , wherein - indicates the bond to the phenyl ring, R4, R6, R12, R14, R15 and R16 are hydrogen, R5 is thiophen-3-yl, and A is -CH2-;

R13 is -F, -OH or -OCH3, R6, R12, R14, R15 and R16 are hydrogen, and A is -C(O)-;

NH
R13 is -NO2, -NH2, or , wherein indicates the bond to the phenyl ring, R4, R5, R6, R12, R14, R15 and R16 are hydrogen, and A is -C(O)-;

R13 is -F, -Cl, or -CF3, R4, R5, R6, R12, R14, R15 and R'6 are hydrogen, and A
is -S(O)2-;

R13 and R14 are -OH, R4, R6, R12, R15 and R16 are hydrogen, R5 is thiophen-3-yl, and A is -CH2-;
R14 is -OH or -OCH3, Ra R6, R12 R13 R15 and R16 are hydrogen, R5 is thiophen-2-yl, and A is -C(O)-;

R14 is -OCH3, R4, R5, R6, R12, R13, R15 and R16 are hydrogen, and A is -C(O)-;
R14 is -Cl, R6, R12, R13, R15 and R16 are hydrogen, and A is -C(O)-;

Q 01--l 5z R14 is +NH , wherein - indicates the bond to the phenyl ring, R4, R5, R6, R12, R13, R'5 and R16 are hydrogen, and A is -CH2-;

R14 is -F, R4, Rs, R6, R12, R13, R15 and R16 are hydrogen, and A is -S- or -S(O)2-;

R14 is -CH3, R5, R6, R12 R13 R15 and R16 are hydrogen, W is 3-(hydroxymethyl)phenyl, and A is -S-;

R12 and R16 are -F, Rs, R6, R13 , R14 and R15 are hydrogen, R4 is 3,5 difluorophenyl, and A is -C(O)-;

R12 is -Cl, R13 is -Cl, R6, R14, RI5 and R16 are hydrogen, and A is -C(O)-;

R12 is -F, R13 is -F, RS, R6, R14 , R15 and R16 are hydrogen, R4 is 3,5 difluorophenyl, and A is -C(O)-;

R12 is -F, R13 is -OH or -OCH3, R4, R6, R14 R'5 and R16 are hydrogen, R5 is thiophen-2-yl, and A
is -C(O)-;

R12 is -F, R13 is -OCH3, R4, R6, R14 , R15 and R16 are hydrogen, R5 is -Br, and A is -C(O)-;
, R12 and R14 are -F, RS R6 R", R" and R16 are hydrogen, R4 is 3,5 difluorophenyl, and A is ~r -C(O)-;
R12 is -CH3, R15 is -F, R6, R13, R14 and R16 are hydrogen and A is -C(O)-;

R12 is -F, R15 is -Cl, R4, R6, R13, R14 and R16 are hydrogen, R5 is thiophen-2-yl and A is -C(O)-;
R12 and R15 are -F, R6, R13, R14 and R16 are hydrogen, and A is -C(O)-;

R12 is halogen, R15 is -OH or -OCH3, R6, R13, R14 and R16 are hydrogen and A
is -C(O)-;
R12 is -F, R15 is -NHS(O)2CH3, R4, R5, R6, R13, R'4 and R16 are hydrogen and A
is -C(O)-;
R13 and R15 are -OCH3, R4, R5, R6, R12, R14 and R16 are hydrogen, and A is -CH2-;

R13 and R15 are -Cl, R4, R5, R6, R12, R14 and R16 are hydrogen, and A is -S(O)2-;

R13 is -OH and R15 is -OH or -OCH3, R4, R6, R12, R14 and R16 are hydrogen, R5 is thiophen-3-yl, and A is -CH2-;

N~/J
Rai R6~ R12, R13, R14, R15 and R16 are hydrogen, A is -S(O)2-, and R5 is NrN
N ) NJ \ I n or wherein indicates the bond to the 5-position of the 7-azaindole ring;

rNIle 12 13 14 1S 16 4 N v R , R6, R , R , R , R" and R are hydrogen, A is -S(O)2-, and R is ~'' , or r'N

'?~ wherein indicates the bond to the 4-position of the 7-azaindole ring;

NH

R , R , R , R , R , R and R are hydrogen, A is -S(O)2-, and R is or N'-~
N~
wherein -' indicates the bond to the 6-position of the 7-azaindole ring;

r N H
R13 is -CN, R4 R6 R12 R'4 R15 and R16 are hydrogen, A is -S(O) 5 i z-, and R is wherein indicates the bond to the 5-position of the 7-azaindole ring;

R12 is -Cl, R14 is -F or hydrogen, R4, R6, R13, R15 and R16 are hydrogen, A is -S(O)z-, and R5 is ( N --~
NJ _ wherein indicates the bond to the 5-position of the 7-azaindole ring;

r N H
R 14 is -NH2, 6 12, 13 16 5 i N
2i R, R , R , R , R15 and R are hydrogen, A is -S(O)2-, and R is L`' wherein indicates the bond to the 5-position of the 7-azaindole ring;

( NH
N
R6, R12, R13, R14, R15 and R16 are hydrogen, A is -S(O)2-, R4 is -Cl, and R5 is wherein indicates the bond to the 5-position of the 7-azaindole ring;

R13 is -F, Ra> R6 R1z R14 R15 and R16 are hydrogen, A is -CHz-, and R5 is 3-hydroxy-phenyl;
R14 is -N(CH3)2, R4, R6, R12 R13 R15 and R16 are hydrogen, A is -CHz-, and R5 is 3-hydroxy-phenyl;

R14 is hydrogen or -Br, R4, R6, R12, R13, R15 and R16 are hydrogen, A is -5-, and R5 is 3-hydroxy-phenyl; and R4, R6, R12, R13, R14, R15 and R16 are hydrogen, A is -C(O)-, and R5 is 3-hydroxy-phenyl.

[01101 In some embodiments of compounds of Formula II, at least one of R'2, R13, R14, R'5 and R16 is -LR24, wherein R24 is substituted methyl, optionally substituted C2-6 alkyl, optionally substituted lower alkenyl, provided, however, that when R24 is optionally substituted lower alkenyl, no alkene carbon thereof is bound to N, S, 0, S(O), S(O)2, C(O) or C(S) of L, optionally substituted lower alkynyl, provided, however, that when R24 is optionally substituted lower alkynyl, no alkyne carbon thereof is bound to N, S, 0, S(O), S(0)2, C(O) or C(S) of L, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted 13 is heteroaryl, provided, however, that R s not OD or N V and N
O

that R14 is not 1_NH , wherein _ indicates the bond to the phenyl ring; in further embodiments, R24 is optionally substituted C2-6 alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, or substituted methyl, wherein methyl is substituted with optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, R5 is other than hydrogen; in further embodiments, R4 and R6 are hydrogen.

[0111] In some embodiments of compounds of Formula II, R12 is other than hydrogen. In some embodiments, R12 is -LR24. In some embodiments, R12 is other than hydrogen and R13, R14, R15 and R'6 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 is -LR24 and R13, R14, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 is -LR24, any three of R13, R14, R15 and R'6 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R13, R14, R15 and R16 is hydrogen. In some embodiments, R12 is -LR24, any two of R13, R14, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R13, R14, R15 and R16 are hydrogen. In some embodiments, R12 is -LR24, any one of R13, R14, R15 and R16 is hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R13, R14, R15 and R16 are hydrogen. In some embodiments, R12 is -LR24, and R13, R14, R15 and R16 are hydrogen. In some embodiments, R12 is other than hydrogen, and R13, R14, R15 and R16 are hydrogen.

[0112] In other embodiments of compounds of Formula II, wherein R12 is other than hydrogen, when R13, R14, R15 and R16 are hydrogen and A is -C(O)-, then R12 is not -CH3, -F, or -OH; and when R4, R5, R6, R13, R14, R15 and R16 are hydrogen, and A is -S(O)2-, then R12 is not -CF3; and when R'2 and R16 are ;F, R5, R6, R13, R14 and R15 are hydrogen, and A is -C(O)-, R4 is not 3,5 difluorophenyl;
and when R12 is halogen, R6, R14, R15 and R16 are hydrogen, and A is -C(O)-, R'3 is not halogen, -OH, or -OCH3; and when R12 and R14 are -F, R5, R6, R13, R15 and R16 are hydrogen, and A is -C(O)-, R4 is not 3,5 difluorophenyl; and when R15 is halogen, -OH or -OCH3, R6, R13, R14 and R16 are hydrogen, and A is -C(O)-, R12 is not halogen; and when R15 is -F, R6, R13, R14 and R16 are hydrogen and A is -C(O)-, R12 is not -CH3; and when R12 is -F, R4, R5, R6, R13, R14 and R16 are hydrogen and A is -C(O)-, R15 is not -NHS(O)2CH3i and when R12 is -Cl, R14 is -F or hydrogen, R4, R6, R13, R15 and R16 are ( N
N _ hydrogen, and A is -S(O)2-, R5 is not wherein indicates the bond to the 5-position of the 7-azaindole ring.

[0113] In some embodiments of any of the above embodiments of compounds of Formula II
wherein R12 is other than hydrogen, R5 is other than hydrogen and R4 and R6 are hydrogen; or R4 is other than hydrogen and R5 and R6 are hydrogen; or R6 is other than hydrogen and R4 and R5 are hydrogen; or R4 and R5 are other than hydrogen and R6 is hydrogen; or R4 and R6 are other than hydrogen and R5 is hydrogen; or R5 and R6 are other than hydrogen and R4 is hydrogen; or R4, R5 and R6 are all other than hydrogen.

[0114] In some embodiments of compounds of Formula II, R13 is other than hydrogen. In some embodiments, R13 is -LR24. In some embodiments, R13 is other than hydrogen and R12, R14, R15 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R13 is -LR24 and R12, R14, R'5 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R13 is -LR24, any three of R12, R14, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R'2, R14, R15 and R16 is hydrogen. In some embodiments, R13 is -LR24, any two of R12, R14, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the the remaining of R12, R14, R15 and R16 are hydrogen. In some embodiments, R13 is -LR24, any one of R12, R14, R'5 and R16 is independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the remaining of R12, R14, R15 and R16 are hydrogen. In some embodiments, R13 is -LR24, and R12, R14, R15 and R16 are hydrogen. In some embodiments, R13 is other than hydrogen, and R12, R14, R15 and R16 are hydrogen.
[0115] In other embodiments of compounds of Formula II, wherein R13 is other than hydrogen, when R4, R6, R12, R14, R15 and R16 are hydrogen, and A is -CH2- or -CH(OH)-, then R13 is not -OH or -OCH3; ; and when R5, R6, R12 R14 R15 and R16 are hydrogen, and A is -CH2-, then R13 is not -OCH3;

and when R13 is 0 , wherein - indicates the bond to the phenyl ring, R4, R6, R12, R14, R15 and R16 are hydrogen, and A is -CH2-, then R5 is not thiophen-3-yl;
and when R6, R12, R14, Rls and R'6 are hydrogen, and A is -C(O)-, then R13 is not -F, -OH, or -OCH3; and when R4, R5, R6, R12, R14, R15 and R16 are hydrogen, and A is -C(O)-, then R13 is not -NO2, -NH2, or yo~
NH
wherein indicates the bond to the phenyl ring; and when R4, R5, R6, R12, R14, R15 and R16 are hydrogen, and A is -S(O)2-, then R13 is not -F, -Cl, or -CF3; and when R'3 and R14 are -OH, R4, R6, R12, R15 and R16 are hydrogen and A is -CH2-, R5 is not thiophen-3-yl; and when R6, R14, R15 and R16 are hydrogen and A is -C(O)-, then R13 and R12 are not both -Cl; and when R12 and R13 are both -F, R5, R6, R14, R15 and R16 are hydrogen and A is -C(O)-, then R4 is not 3,5-difluorophenyl; and when R13 is -OH or -OCHs, R12 is -Fa R4 R6 R", R15 and R 16 are hydrogen, and A is -C O - then R5 is not thio hen-2- l; and when R13 is -OCH3, R12 is -F
Ra R6 R14 R15 and R16 are hydrogen, and A is -C(O)-, then R5 is not -Br; and when R6, R12, R14 and R15 are hydrogen, R16 is -CH3 and A is -C(O)-, then R13 is not -F; and when R13 is -Cl, R16 is -F, R4, R6, R12, R14 and R'5 are hydrogen, and A is -C(O)-, then R5 is not thiophen-2-yl; and when R6, R12, R14 and R15 are hydrogen and A is -C(O)-, then R13 and R16 are not both -F; and when R6, R12, R14 and R15 are hydrogen, R16 is halogen, and A is -C(O)-, then R13 is not -OH or -OCH3; and when R4, R5, R6, R12, R14 and R15 are hydrogen, R13 is -NHS(O)2CH3> and A is -C(O)-, then R16 is not -F; ; and when R4, RS, R6, R12 , R14 and R16 are hydrogen, and A is -CH2-, then R13 and R15 are not both -OCH3; and when R4, R5, R6, R12, R14 and R16 are hydrogen, and A is -S(O)2-, then R13 and R1S are not both -Cl; and when R13 is -OH, R15 is -OH or -OCH3, R4, R6, R12, R14 and R16 are hydrogen, and A is -CH2-, then R5 is not thiophen-3-yl;
and when R13 is -F, R4, R6, R12, R14, R15 and R16 are hydrogen, and A is -CH2-, then R5 is not 3-hYdroxY-phenY~ l; and when R13 is -CN, Ra R6, R12 a R'4 , R15 and R16 are hydrogen, A is -S(O)2-, then (NH
N
R5 is not wherein indicates the bond to the 5-position of the 7-azaindole ring.
[0116] In some embodiments of any of the above embodiments of compounds of Formula II
wherein R13 is other than hydrogen, R5 is other than hydrogen and R4 and R6 are hydrogen, or R4 is other than hydrogen and R5 and R6 are hydrogen, or R6 is other than hydrogen and R4 and R5 are hydrogen, or R4 and R5 are other than hydrogen and R6 is hydrogen, or R4 and R6 are other than hydrogen and R5 is hydrogen, or R5 and R6 are other than hydrogen and R4 is hydrogen, or R4, R5 and R6 are other than hydrogen.

[0117] In some embodiments of compounds of Formula II, R14 is other than hydrogen. In some embodiments, R14 is -LR24. In some embodiments, R14 is other than hydrogen and R12, R13, R15 and R16 are each independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R14 is -LR24, and R12, R13, R's and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R14 is -LR24, any three of R12, R13, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the remaining of R12, R13, R's and R16 is hydrogen. In some embodiments, R'4 is -LR24, any two of R12, R13, R's and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the remaining of R12, R13, R's and R16 are hydrogen. In some embodiments, R14 is -LR24, any one of R12, R13, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the remaining of R'2, R13, R15 and R16 are hydrogen. In some embodiments, R14 is -LR24, and R12, R13, R15 and R16 are hydrogen. In some embodiments, R14 is other than hydrogen, and R12, R13, R15 and R16 are hydrogen.
[0118] In other embodiments of compounds of Formula II, wherein R14 is other than hydrogen, when R4, R6, R12, R13, R15 and R16 are hydrogen, R5 is thiophen-2-yl and A is -C(O)-, then R14 is not -OH, or -OCH3; and when R4 Rs R6 R12 R13, R's and R16 are hydrogen and A is -C
O - then R14 is not -OCH3; and when R6, R12, R13, R15 and R16 are hydrogen and A is -C(O)-, then R14 is not -Cl; and when R4, Rs, R6, R12, R13, R15 and R16 are hydrogen and A is -CH2-, then R14 is not + NH , wherein - indicates the bond to the phenyl ring; and when R4, Rs, R6, R12, R13, R15 and R16 are hydrogen and A is -S- or -S(O)2-, then R14 is not -F; and when Rs, R6, R12, R13, R15 and R16 are hydrogen, R4 is 3-(hydroxymethyl)phenyl and A is -S-, then R14 is not -CH3;
and when Rs R6, R12, R13 R15 and R16 are hydrogen, R4 is 3,5-difluorophenyl and A is -C(O)-, R12 and R14 are not both -F; and when R14 is -N(CH3)2, R4, R6, R12, R13, R'5 and R16 are hydrogen, and A is -CH2-, then Rs is not 3-hydroxy-phenyl; and when R14 is -Br, R4, R6, R12, R13, R's and R16 are hydrogen, and A is -S-, then Rs is not 3-hydroxy-phenyl; and when R12 is -Cl, R14 is -F, R4, R6, R13, N( R15 and R16 are hydrogen, and A is -S(O)2-, then R5 is not wherein indicates the bond to the 5-position of the 7-azaindole ring; and when R14 is -NH2, R4, R6, R12, R'3, R'5 and R16 ~
r NH
N
are hydrogen, and A is -S(O)2-, then R5 is not ~' , wherein indicates the bond to the 5-position of the 7-azaindole ring; and when R4, R6, R12, R's and R16 are hydrogen, R13 and R14 are -OH, and A is -CH2-, then Rs is not thiophen-3-yl.

[0119] In some embodiments of any of the above embodiments of compounds of Formula II
wherein R14 is other than hydrogen, R5 is other than hydrogen and R4 and R6 are hydrogen, or R4 is other than hydrogen and R5 and R6 are hydrogen, or R6 is other than hydrogen and R4 and R5 are hydrogen, or R4 and R5 are other than hydrogen and R6 is hydrogen, or R4 and R6 are other than hydrogen and R5 is hydrogen, or R5 and R6 are other than hydrogen and R4 is hydrogen, or R4, R5 and R6 are other than hydrogen.

[0120] In some embodiments of compounds of Formula II, R12 and R16 are other than hydrogen. In some embodiments, R12 and R16 are independently -LR24. In some embodiments, R12 and R16 are other than hydrogen and R13, R14 and R1S are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 and R16 are independently -LR24 and R13, R14 and R15 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 and R16 are independently -LR24, any two of R13, R14 and R15 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the remaining of R13, R14 and R15 is hydrogen. In some embodiments, R12 and R16 are independently -LR24, any one of R13, R14 and R'5 is independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the remaining of R13, R14 and R15 are hydrogen. In some embodiments, R12 and R16 are independently -LR24, and R13, R14 and R15 are hydrogen. In some embodiments, R12 and R16 are other than hydrogen, and R13, R'4 and R15 are hydrogen. In other embodiments, wherein R12 and R16 are other than hydrogen, when R5, R6, R13, R14 and R15 are hydrogen and A is -C(O)-, then R12 and R16 are not both -F.

[0121] In some embodiments of any of the above embodiments of compounds of Formula II
wherein R12 and R' 6 is other than hydrogen, R5 is other than hydrogen and R4 and R6 are hydrogen, or R4 is other than hydrogen and R5 and R6 are hydrogen, or R6 is other than hydrogen and R4 and R5 are hydrogen, or R4 and R5 are other than hydrogen and R6 is hydrogen, or R4 and R6 are other than hydrogen and R5 is hydrogen, or R5 and R6 are other than hydrogen and R4 is hydrogen, or R4, R5 and R6 are other than hydrogen.

[0122] In some embodiments of compounds of Formula II, R12 and R13 are other than hydrogen. In some embodiments, R12 and R'3 are independently -LR24. In some embodiments, R12 and R13 are other than hydrogen and R14, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 and R13 are independently -LR24, and R14, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 and R13 are independently -LR24, any two of R14, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the remaining of R14, R15 and R16 is hydrogen. In some embodiments, R12 and R13 are independently -LR24, any one of R14, R15 and R16 is independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the remaining of R14, R15 and R16 are hydrogen. In some embodiments, R12 and R13 are independently -LR24, and R14, R15 and R16 are hydrogen. In some embodiments, R12 and R13 are other than hydrogen and R14, R15 and R16 are hydrogen. In other embodiments, wherein R12 and R13 are other than hydrogen, when R6, R'4, R'5 and R16 are hydrogen, A is -C(O)-, and R13 is halogen, -OH, or -OCH3, then R'2 is not halogen. In alternate embodiments, when R12 and R13 are other than hydrogen, R6, R14, R15 and R'6 are hydrogen and A is -C(O)-, then both R12 and R13 are not halogen; and when R12 and R13 are other than hydrogen, R4, R6, R14, R'5 and R16 are hydrogen, and A is -C(O)-, R12 is -F and R13 is -OH or -OCH3, then R5 is not -Br or thiophen-2-yl.

[01231 In some embodiments of any of the above embodiments of compounds of Formula II
wherein R12 and R13 is other than hydrogen, R5 is other than hydrogen and R4 and R6 are hydrogen, or R4 is other than hydrogen and R5 and R6 are hydrogen, or R6 is other than hydrogen and R4 and R5 are hydrogen, or R4 and R5 are other than hydrogen and R6 is hydrogen, or R4 and R6 are other than hydrogen and R5 is hydrogen, or R5 and R6 are other than hydrogen and R4 is hydrogen, or R4, R5 and R6 are other than hydrogen.

[01241 In some embodiments of compounds of Formula II, R12 and R14 are other than hydrogen. In some embodiments, R12 and R14 are independently -LR24. In some embodiments, R12 and R14 are other than hydrogen and R13, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 and R14 are independently -LR24, and R13, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 and R14 are independently -LR24, and any one of R13, R15 and R16 is independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the remaining of R13, R15 and R16 is hydrogen. In some embodiments, R12 and R14 are independently -LR24, and any two of R'3, R'5 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R13, R15 and R16 is hydrogen. In some embodiments, R12 and R14 are independently -LR24 and R13, R15 and R16 are hydrogen. In some embodiments, R12 and R14 are other than hydrogen and R13, R15 and R16 are hydrogen. In some embodiments, wherein R12 and R14 are other than hydrogen, when Rs, R6, R'3, R'5 and R16 are hydrogen, A is -C(O)-, and R4 is 3,5 difluorophenyl, then R12 and R14 are not both -F; and when R12 is -Cl, R14 is -F, R4, R6, R13, R'5 and R16 are hydrogen, and A is -S(0)2-, then R5 is not I ~ N
czN") , wherein indicates the bond to the 5-position of the 7-azaindole ring.
[0125] In some embodiments of any of the above embodiments of compounds of Formula II
wherein R12 and R14 is other than hydrogen, R5 is other than hydrogen and R4 and R6 are hydrogen, or R4 is other than hydrogen and R5 and R6 are hydrogen, or R6 is other than hydrogen and R4 and R5 are hydrogen, or R4 and R5 are other than hydrogen and R6 is hydrogen, or R4 and R6 are other than hydrogen and R5 is hydrogen, or R5 and R6 are other than hydrogen and R4 is hydrogen, or R4, R5 and R6 are other than hydrogen.

[0126] In some embodiments of compounds of Formula II, R12 and R15 are other than hydrogen. In some embodiments, R12 and R15 are independently -LR24. In some embodiments, R12 and R15 are other than hydrogen and R13, R14 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 and R15 are independently -LR24 and R13, R14 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R12 and R15 are independently -LR24, any two of R13, R14 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R13, R14 and R16 is hydrogen. In some embodiments, R12 and R15 are independently -LR24, any one of R13, R14 and R16 is hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R13, R14 and R16 are hydrogen. In some embodiments, R12 and R15 are independently -LR24 and R13, R14 and R16 are hydrogen. In some embodiments, R12 and R15 are other than hydrogen and R13, R14 and R16 are hydrogen. In other embodiments, wherein R12 and R15 are other than hydrogen, when R6, R13, R14 and R16 are hydrogen and A is -C(O)-, then R12 and R15 are not both halogen; and when R6, R13, R14 and R16 are hydrogen, A
is -C(O)-, and R12 is -CH3, then R'5 is not -F; and when R6, R13, R'4 and R16 are hydrogen, A is -C(O)-, and R12 is halogen, then R15 is not -OH or -OCH3i and when R4, R5, R6, R13, R14 and R16 are hydrogen, A is -C(O)-, and R12 is -F then R15 is not NHS(O)2CH3.

[0127] In some embodiments of any of the above embodiments of compounds of Formula II
wherein R12 and R15 is other than hydrogen, R5 is other than hydrogen and R4 and R6 are hydrogen, or R' is other than hydrogen and R5 and R6 are hydrogen, or R6 is other than hydrogen and R4 and R5 are hydrogen, or R4 and R5 are other than hydrogen and R6 is hydrogen, or R4 and R6 are other than hydrogen and R5 is hydrogen, or R5 and R6 are other than hydrogen and R4 is hydrogen, or R4, R5 and R6 are other than hydrogen.

[0128] In some embodiments of compounds of Formula II, R13 and R14 are other than hydrogen. In some embodiments, R13 and R14 are independently -LR24. In some embodiments, R13 and R14 are other than hydrogen and RI2, RI5 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R13 and R14 are independently -LR24 and R12, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R13 and R14 are independently -LR24, any two of R12, RI5 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, R15 and R16 is hydrogen. In some embodiments, R13 and R14 are independently -LR24, any one of R12, RI5 and R16 is hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, RI5 and R16 are hydrogen. In some embodiments, R13 and R14 are independently -LR24 and R12, RI5 and R16 are hydrogen. In some embodiments, R13 and R14 are other than hydrogen and R12, RI5 and R16 are hydrogen. In other embodiments, wherein R13 and R14 are other than hydrogen, when R4, R6, R12, RI5 and R16 are hydrogen, A is -CH2-, and R5 is thiophen-3-yl, then R13 and R14 are not both -OH.

[0129] In some embodiments of any of the above embodiments of compounds of Formula II
wherein R13 and R14 is other than hydrogen, R5 is other than hydrogen and R4 and R6 are hydrogen, or R4 is other than hydrogen and R5 and R6 are hydrogen, or R6 is other than hydrogen and R4 and R5 are hydrogen, or R4 and R5 are other than hydrogen and R6 is hydrogen, or R4 and R6 are other than hydrogen and R5 is hydrogen, or R5 and R6 are other than hydrogen and R4 is hydrogen, or R4, R5 and R6 are other than hydrogen.

[0130] In some embodiments of compounds of Formula II, R13 and R15 are other than hydrogen. In some embodiments, R13 and R15 are independently -LR24. In some embodiments, R13 and R15 are other than hydrogen and R12, R14 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R13 and R15 are independently -LR24 and R12, R14 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, R13 and R15 are independently -LR24, two of R12, R'4 and R'6 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, R14 and R16 is hydrogen. In some embodiments, R13 and R15 are independently -LR24, one of R12, R14 and R16 is hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, R14 and R16 are hydrogen. In some embodiments, R13 and R15 are independently -LR24 and R12, R14 and R16 are hydrogen. some embodiments, R13 and R15 are other than hydrogen and R12, R14 and R16 are hydrogen. In other embodiments, wherein R13 and R1S are other than hydrogen, when R4, R5, R6, R12, R14 and R16 are hydrogen and A is -CH2-, then R13 and R15 are not both -OCH3; and when R4, R5, R6, R12, R14 and R16 are hydrogen and A is -S(O)z-, then R13 and R15 are not both -Cl; and when R4 R6, R12 > R14 and R16 are hydrogen, A is -CH2-, R13 is -OH and R15 is -OH or -OCH3, then R5 is not thiophen-3-yl.

[01311 In some embodiments of any of the above embodiments of compounds of Formula II
wherein R13 and R15 is other than hydrogen, R5 is other than hydrogen and R4 and R6 are hydrogen, or R4 is other than hydrogen and R5 and R6 are hydrogen, or R6 is other than hydrogen and R4 and R5 are hydrogen, or R4 and R5 are other than hydrogen and R6 is hydrogen, or R4 and R6 are other than hydrogen and R5 is hydrogen, or R5 and R6 are other than hydrogen and R4 is hydrogen, or R4, R5 and R6 are other than hydrogen.

[01321 In some embodiments of compounds of Formula II, R13 is -LR24, provided, however, that when A is -CH2-, -CH(OH)- or -C(O)-, and R12, R14, R15, and R16 are hydrogen, then R13 is not -OH, -OCH3, or , wherein - indicates the bond to the phenyl ring; and when W, R5, R6, R12, R14, R15, and R16 are hydrogen and A is -C(O)-, R13 is not NH2, or NH
wherein indicates the bond to the phenyl ring; and when R4, R5, R6, R12, R14 and R16 are hydrogen and A is -CH2-, R13 and R15 are not both -OCH3;
and when R4, R6, R12, R14 and R16 are hydrogen, A is -CH2-, R5 is thiophen-3-yl, R13 is -OH, R15 is not -OH or -OCH3; and when R4, R6, R12, R15 and R16 are hydrogen, A is -CH2-, and R5 is thiophen-3-yl, R13 and R14 are not both -OH; and when R4, R6, R14, R15 and R16 are hydrogen and A is -C(O)-, R12 is -F, R5 is -Br or thiophen-2-yl, R13 is not -OH or -OCH3; and when R12, R14, and R15 are hydrogen, A is -C(O)-, and R16 is halogen, R13 is not -OH or -OCH3; and when R4, R5, R6, R12, R14, and R15 are hydrogen, A is -C(O)-, and R16 is -F, R13 is not -NHS(O)2CH3. In further embodiments, R13 is -LR24 and L is -NHS(O)2CH2-, -0-, or -0-CH2-, R24 is not H, or when L is -NHS(O)2- or -0-, R24 is not CH3. In further embodiments, where R13 is -LR24, one of R12, R14, R15, and R16 is other than hydrogen and the remaining of R12, R14, R15, and R16 are hydrogen. In further embodiments, where R13 is -LR24, two of R12, R14, R15, and R16 are independently other than hydrogen and the remaining two of R'2, R14, R15, and R16 are hydrogen. In some embodiments where R13 is -LR24, R14 and R15 are hydrogen, and at least one of R12 and R16 is other than hydrogen. In further embodiments, where R13 is -LR24, R14 and R15 are hydrogen, and at least one of R12 and R16 is other than hydrogen, L is -(alk)a NR25-(alk)b-, -(alk)a-C(O)NR25-(alk)b-, -(alk)a-OC(O)NR25-(alk)b-, -(alk)a-OC(S)NR25-(alk)b-, -(alk)a-C(S)NR25-(alk)b-, -(alk)a S(O)2NR25-(alk)b-, -(alk)a NR25C(O)-(alk)b-, -(alk)a NR25C(S)-(alk)b-, -(alk)a-NR25C(O)NR25-(alk)b-, -(alk)a-NR25C(S)NR25-(alk)b-, -(alk)a NR25C(O)O-(alk)b-, -(alk)a-NR25C(S)O-(alk)b-, -(alk)a NR25S(O)2-(alk)b-, or -(a1k)a-NR25S(O)2NR25-(alk)b-, wherein a, b, R25 and alk are as defined for Formula I.

[0133] In further embodiments of compounds of Formula II, where R13 is -LR24, R14 and R15 are hydrogen, and at least one of R12 and R16 is other than hydrogen, L is NR25-, -NR25-(alk)b-, -C(O)NR25-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25-(alk)b-, -C(S)NR25-(alk)b-, -S(O)2NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR25C(O)NR25-(alk)b-, -NR25C(S)NR25-(alk)b-, -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-.

[0134] In further embodiments of compounds of Formula II, where R13 is -LR24, R14 and R15 are hydrogen, and at least one of R12 and R16 is other than hydrogen, L is -NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR25C(O)NR25-(alk)b-, -NR25C(S)NR25-(alk)b-, -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-. In further embodiments where R13 is -LR24, R14 and R15 are hydrogen, and at least one of R12 and R16 is other than hydrogen, L is -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR25S(O)2-(alk)b-, -NR25C(O)NR25-(alk)b-, -NR25C(S)NR25-(a1k)b-, or -NR25S(O)2NR25-(alk)b-. In further embodiments where R13 is -LR24, R14 and R15 are hydrogen, and at least one of R12 and R16 is other than hydrogen, L is -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -OC(O)NR25-(alk)b-, or -OC(S)NR25-(alk)b-. In some embodiments of any of the above embodiments where R13 is -LR24, R5 is other than hydrogen and R4 and R6 are hydrogen, or R4 is other than hydrogen and R5 and R6 are hydrogen, or R6 is other than hydrogen and R4 and R5 are hydrogen, or R4 and R5 are other than hydrogen and R6 is hydrogen, or R4 and R6 are other than hydrogen and R5 is hydrogen, or R5 and R6 are other than hydrogen and R4 is hydrogen, or R4, R5 and R6 are other than hydrogen.

[0135] In some embodiments of compounds of Formula II, A is -CRaRb- or -C(O)-, R13 is -LR24, R14 and R15 are hydrogen, R12 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, wherein one of R12 and R16 is other than hydrogen, and wherein L is -NR25-(alk)b-, -C(O)NR25-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25-(alk)b-, -C(S)NR25-(alk)b-, -S(O)2NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR25C(O)NR25-(alk)b-, -NR25C(S)NR25-(alk)b -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein b and R' are as defined for Formula I, alk is C1_3 alkylene optionally substituted with fluoro or optionally fluoro substituted lower alkyl, and R24 is hydrogen, provided, however, that said hydrogen would not be attached to S(O)2-, optionally fluoro substituted lower alkyl, cycloalkyl, heterocycloalkyl, aryl or heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl and heteroaryl are optionally substituted with halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and Ra and Rb are independently hydrogen, halogen, optionally fluoro substituted lower alkyl or optionally fluoro substituted lower alkoxy, provided, however, that when R4, R5, R6, R12, R14, and R15 are hydrogen, A
is -C(O)-, and R16 is -F, then R13 is not -NHS(O)2CH3. In other embodiments, R12 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In other embodiments, A is -CH2-. In other embodiments, A is -C(O)-. In other embodiments, R4 is other than hydrogen and R5 and R6 are hydrogen. In other embodiments, A is -CH2-, R4 is other than hydrogen and R5 and R6 are hydrogen.
In other embodiments, A is -C(O)-, R4 is other than hydrogen and R5 and R6 are hydrogen. In other embodiments, R5 is other than hydrogen and R4 and R6 are hydrogen. In other embodiments, A is -CH2-, R5 is other than hydrogen and R4 and R6 are hydrogen. In other embodiments, A is -C(O)-, R5 is other than hydrogen and R4 and R6 are hydrogen. In other embodiments, R6 is other than hydrogen and R4 and R5 are hydrogen. In other embodiments, A is -CH2-, R6 is other than hydrogen and R4 and R5 are hydrogen. In other embodiments, A is -C(O)-, R6 is other than hydrogen and R4 and R5 are hydrogen. In other embodiments, R4 and R5 are other than hydrogen and R6 is hydrogen. In other embodiments, R4 and R6 are other than hydrogen, and R5 is hydrogen. In other embodiments, R4 and R5 are hydrogen, and R6 is other than hydrogen. In other embodiments, R4, R5, and R6 are other than hydrogen.

[0136] In some embodiments of compounds of Formula II, A is -CRaRb- or -C(O)-, R13 is -LR24, R14 and R15 are hydrogen, R12 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, wherein one of R12 and R16 is other than hydrogen, and wherein L is -NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR25COO NR25-(alk)b-, -NR 25C(S)NR 2s-(alk)b-, -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein b and R25 are as defined for Formula I, alk is C1_3 alkylene optionally substituted with fluoro or optionally fluoro substituted lower alkyl, R24 is hydrogen provided, however, that said hydrogen would not be attached to S(O)2-, optionally fluoro substituted lower alkyl, cycloalkyl, heterocycloalkyl, aryl or heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl and heteroaryl are optionally substituted with halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, and optionally fluoro substituted lower alkylthio, Ra and Rb are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, or optionally fluoro substituted lower alkoxy, one of R4 and R5 is other than hydrogen and R6 is hydrogen. In other embodiments, R16 is hydrogen. In other embodiments, R12 is hydrogen. In other embodiments, R12 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In other embodiments, A is -CH2-. In other embodiments, A is -C(O)-. In other embodiments, R4 is other than hydrogen and R5 and R6 are hydrogen. In other embodiments, A is -CH2-, R4 is other than hydrogen and R5 and R6 are hydrogen. In other embodiments, A is -C(O)-, R4 is other than hydrogen and R5 and R6 are hydrogen.
In other embodiments, R5 is other than hydrogen and R4 and R6 are hydrogen. In other embodiments, A is -CH2-, R5 is other than hydrogen and R4 and R6 are hydrogen. In other embodiments, -C(O)-, R5 is other than hydrogen and R4 and R6 are hydrogen. In other embodiments, R6 is other than hydrogen and R4 and R5 are hydrogen. In other embodiments, A is -CH2-, R6 is other than hydrogen and R4 and R5 are hydrogen. In other embodiments, A is -C(O)-, R6 is other than hydrogen and R4 and R5 are hydrogen. In other embodiments, R4 and R5 are other than hydrogen and R6 is hydrogen. In other embodiments, R4 and R6 are other than hydrogen and R5 is hydrogen. In other embodiments, R4 and R5 are hydrogen and R6 is other than hydrogen. In other embodiments, R4, R5, and R6 are other than hydrogen.

[0137] In some embodiments of compounds of Formula II, A is -CR'R1i- or -C(O)-, R13 is -LR24, R12 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, one of R14, R15 and R16 are halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the other two are hydrogen, L is -NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, -NR25C(O) NR25-(alk)b-, 'NR2 5C(S)NR25-(alk)b-, -NR25C(O)O-(alk) b'v -NR25C(S)O-(alk)b-, NRZ5S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein b and R25 are as defined for Formula I, alk is C1_3 alkylene optionally substituted with fluoro or optionally fluoro substituted lower alkyl, R24 is hydrogen provided, however, that said hydrogen would not be attached to S(O) or S(0)2, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, R' and Rb are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, or optionally fluoro substituted lower alkoxy, one of R4 and R5 is other than hydrogen and R6 is hydrogen. In other embodiments, R4 is hydrogen and R5 is other than hydrogen. In other embodiments, R5 is hydrogen and R4 is other than hydrogen. In other embodiments, A is -CH2-, R5 is hydrogen and R4 is other than hydrogen. In other embodiments, A is -C(O)-, R5 is hydrogen and R4 is other than hydrogen. In other embodiments, A is -CH2-, R4 is hydrogen and R5 is other than hydrogen. In other embodiments, A is -C(O)-, R4 is hydrogen and R5 is other than hydrogen.

[0138] In some embodiments of compounds of Formula II, A is -CRaRb- or -C(O)-, R13 is -LR24, R12, Rio, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, where at least one of R'2, R14, R15 and R16 is other than hydrogen, L is NR25C(O)-(alk)b-, -NR75C(S)-(alk)b-, -NR25S(O)2-(alk)b-, -NR25C(O)NR25-(alk)b-, -NR 2sC(S)NR 2s-(alk)b-, or -NR
25S(O)2NR2s -(alk)b-, wherein b and R25 are as defined for Formula I, alk is C1.3 alkylene optionally substituted with fluoro or optionally fluoro substituted lower alkyl, R24 is hydrogen provided, however, that said hydrogen would not be attached to S(O)2, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, Ra and Rb are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, or optionally fluoro substituted lower alkoxy, provided, however, that when R4, R5, R6, R12, R14, and R15 are hydrogen, A is -C(O)-, and R16 is -F, then R13 is not -NHS(O)2CH3. In further embodiments, R12 is other than hydrogen and R14, R15 and R16 are hydrogen. In further embodiments, R16 is other than hydrogen and R12, R14 and R15 are hydrogen. In further embodiments, at least two of R12, R14, R15 and R16 are other than hydrogen. In further embodiments, R12 and R16 are other than hydrogen and R14 and R15 are hydrogen. In further embodiments, R5 is other than hydrogen and R4 and R6 are hydrogen.
In further embodiments, R4 is other than hydrogen and R5 and R6 are hydrogen.

[0139] In some embodiments of compounds of Formula II, A is -CRaRR- or -C(O)-, R13 is -LR24, R'2, R14, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, where at least one of R12, R14, R15 and R16 is other than hydrogen, L is NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -OC(O)NR25-(alk)b-, or -OC(S)NR25-(alk)b-, wherein b and R25 are as defined for Formula I, alk is C1_3 alkylene optionally substituted with fluoro or optionally fluoro substituted lower alkyl, R24 is hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, Ra and Rb are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, and optionally fluoro substituted lower alkoxy. In further embodiments, R12 is other than hydrogen and R14, R15 and R16 are hydrogen. In further embodiments, R16 is other than hydrogen and R12, R15 and R16 are hydrogen. In further embodiments, at least two of R12, R14, R15 and R16 are other than hydrogen. In further embodiments, R12 and R16 are other than hydrogen and R14 and R15 are hydrogen. In further embodiments, R5 is other than hydrogen and R4 and R6 are hydrogen. In further embodiments, R4 is other than hydrogen and R5 and R6 are hydrogen.

[0140] In some embodiments of any of the above embodiments of compounds of Formula II

13 - 24 24 -OCH3, -Y
wherein R is LR , -LR is not -NH2, -OH, NHS(O)2CH3, , or aI

NH
, wherein indicates the bond to the phenyl ring.

[0141] In some embodiments of compounds of Formula II, R14 is -LR24, provided, however, that when R4, R6, R12, R13, R15 and R16 are hydrogen, R5 is hydrogen or thiophen-2-yl and A is -C(O)-, then R14 is not -OH, or -OCH3; = and when R4a Rs R, R R13 R15 and R16 are hydrogen and A is -CH2-, R14 o N
N
is not 4 NH , wherein - indicates the bond to the phenyl ring, when R4, R6, R12, R15 and R16 are hydrogen, A is -CH2-, and R5 is thiophen-3-yl, R13 and R14 are not both -OH;
and when R5, R6, R12, R13, R15 and R16 are hydrogen and A is -S-, R14 is not -CH3; and when R14 is -N(CH3)2, R4 R6, R12, R13, R15 and R16 are hydrogen, and A is -CH2-, then R5 is not 3-hydroxy-phenyl;
and when R14 is -NH2, R4, R6, R12, R13, R15 and R16 are hydrogen, and A is -S(O)2-, then R5 is not r NH
N
cz' , wherein indicates the bond to the 5-position of the 7-azaindole ring. In further embodiments, when L is -0-, R24 is not H or CH3i and when L is -OCH2-, R24 is not H. In some embodiments, one of R12, R13, R15, and R16 is other than hydrogen and the others are hydrogen. In some embodiments, at least one of R12, R13, R15, and R16 are other than hydrogen. In some embodiments, two of R12, R13, R15, and R16 are other than hydrogen and the others are hydrogen. In some embodiments, at least two of R12, R13, R'5, and R16 are other than hydrogen. In a some embodiments, at least three of R12, R13, R15, and R16 are other than hydrogen.
In some embodiments, R12, R13, R'5, and R16 are other than hydrogen. In some embodiments, one of R12, R13, R15 and R16 is hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the others of R12, R13, R15 and R16 are hydrogen. In some embodiments, any two of R12, R13, R15 and R16 is hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, R13, Rl5 and R16 are hydrogen. In some embodiments, R14 is -OR24, where R24 is optionally substituted C2_6 alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R14 is -O-alk-R24, where R24 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, R14 is -SR24, Where R24 is hydrogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R14 is -S-alk-R24, Where R24 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, R14 is -NHR24, where R24 is hydrogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R14 is -NH-alk-R24, where R24 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, A is -CH2- or -C(O)-.
In some embodiments, R5 is other than hydrogen and R4 and R6 are hydrogen. In some embodiments, R4 is other than hydrogen and R5 and R6 are hydrogen. In some embodiments, R6 is other than hydrogen and R4 and R5 are hydrogen. In some embodiments, A is -CH2-, R5 is other than hydrogen and R4 and R6 are hydrogen. In some embodiments, A is -CH2-, R4 is other than hydrogen and R5 and R6 are hydrogen. In some embodiments, A is -CH2-, R6 is other than hydrogen and R4 and R5 are hydrogen.
In some embodiments, A is -C(O)-, R5 is other than hydrogen and R4 and R6 are hydrogen. In some embodiments, A is -C(O)-, R4 is other than hydrogen and R5 and R6 are hydrogen. In some embodiments, A is -C(O)-, R6 is other than hydrogen and R4 and R5 are hydrogen.

[0142] In some embodiments of compounds of Formula II, R14 is -OR24 where R24 is optionally substituted C2_6 alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R14 is -SR24 where R24 is hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R14 is -NHR24, where R24 is hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, any one of R12, R'3, R15 and R16 is hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, R13, R15 and R16 are hydrogen. In some embodiments, two of R12, R13, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining two of R12, R'3, R15 and R16 are hydrogen. In some embodiments, R12 is hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and R13, R15 and R16 are hydrogen. In some embodiments, A is -CH2- or -C(O)-. In some embodiments, R5 is other than hydrogen and R4 and R6 are hydrogen. In some embodiments, R4 is other than hydrogen and R5 and R6 are hydrogen. In some embodiments, R6 is other than hydrogen and R4 and R5 are hydrogen. In some embodiments, A is -CH2-, R5 is other than hydrogen and R4 and R6 are hydrogen. In some embodiments, A is -CH2-, R4 is other than hydrogen and Rs and R6 are hydrogen. In some embodiments, A is -CH2-, R6 is other than hydrogen and R4 and Rs are hydrogen. In some embodiments, A is -C(O)-, R5 is other than hydrogen and R4 and R6 are hydrogen. In some embodiments, A is -C(O)-, R4 is other than hydrogen and R5 and R6 are hydrogen. In some embodiments, A is -C(O)-, R6 is other than hydrogen and R4 and R5 are hydrogen.
[0143] In some embodiments of compounds of Formula II, R14 is -LR24, where L
is NR25-(alk)b-, -C(O)NR25-(alk)b-, -OC(O)NR25-(alk)b-, -OC(S)NR25-(alk)b-, -C(S)NR25-(alk)b-, -S(O)2NR25-(alk)b-, -NR25C(O)-(alk)b-, -NR25C(S)-(alk)b-, NR25C O NR25- alk b-, -NR25C S NR25-/alk -NR25C(O)O-(alk)b-, -NR25C(S)O-(alk)b-, -NR25S(O)2-(alk)b-, or -NR25S(O)2NR25-(alk)b-, wherein b, alk and R25 are as defined for Formula I, and R24 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, any one of R12, R'3, R'5, and R16 is other than hydrogen and the remaining of R12, R13, R15, and R16 are hydrogen. In some embodiments, at least one of R12, R13, R'5, and R'6 are other than hydrogen. In some embodiments, any two of R12, R13, R15, and R16 are other than hydrogen and the remaining of R12, R13, R15, and R16 are hydrogen. In some embodiments, at least two of R12, R13, R15, and R16 are other than hydrogen. In a some embodiments, at least three of R12, R'3, R15, and R'6 are other than hydrogen. In a some embodiments, R12, R13, R15, and R16 are other than hydrogen. In some embodiments, any one of R'2, R13, R15 and R16 is hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the remaining of R12, R13, R's, and R16 are hydrogen. In some embodiments, two of R'2, R13, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the others of R12, R13, R's and R16 are hydrogen. In some embodiments, A is selected from -CH2- and -C(O)-. In some embodiments, R5 is other than hydrogen and R4 and R6 are hydrogen. In some embodiments, R4 is other than hydrogen and R5 and R6 are hydrogen. In some embodiments, R6 is other than hydrogen and R4 and Rs are hydrogen. In some embodiments, A is -CH2-, R5 is other than hydrogen and R4 and R6 are hydrogen. In some embodiments, A is -CH2-, R4 is other than hydrogen and Rs and R6 are hydrogen. In some embodiments, A is -CH2-, R6 is other than hydrogen and R4 and R5 are hydrogen. In some embodiments, A is -C(O)-, R5 is other than hydrogen and R4 and R6 are hydrogen. In some embodiments, A is -C(O)-, R4 is other than hydrogen and R5 and R6 are hydrogen.
In some embodiments, A is -C(O)-, R6 is other than hydrogen and R4 and Rs are hydrogen.

[0144] In some embodiments of any of the above embodiments of compounds of Formula II

o i I N

N
wherein R14 is -LR24, -LR24 is not NH2i -OH, -OCH3, -N(CH3)2, or-NFI

wherein - indicates the bond to the phenyl ring.

[0145] In some embodiments of compounds of Formula II, R13 and R15 are halogen, optionally fluoro substituted lower alkyl, -OR24, where R24 is optionally substituted C2.6 alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, -O-alk-R24, where R24 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, -SR24, where R24 is hydrogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, -S-alk-R24, where R24 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, -NHR24, Where R24 is hydrogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or -NH-alk-R24, Where R24 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, at least one of R12, R14 and R16 is other than hydrogen. In some embodiments, at least two of R12, R14 and R16 are other than hydrogen. In some embodiments, R12, R14 and R16 are other than hydrogen. In some embodiments, any one of R12, R14 and R16 is hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, R14 and R16 are hydrogen. In some embodiments, any two of R12, R14 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, R14 and R16 is hydrogen. In some embodiments, R12, R14 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -CH2- or -C(O)-. In some embodiments, R5 is other than hydrogen and R4 and R6 are hydrogen. In some embodiments, R4 is other than hydrogen and R5 and R6 are hydrogen. In some embodiments, R6 is other than hydrogen and R4 and R5 are hydrogen. In some embodiments, A is -CH2-, R5 is other than hydrogen and R4 and R6 are hydrogen. In some embodiments, A is -CH2-, R4 is other than hydrogen and R5 and R6 are hydrogen. In some embodiments, A is -CH2-, R6 is other than hydrogen and R4 and R5 are hydrogen. In some embodiments, A is -C(O)-, R5 is other than hydrogen and R4 and R6 are hydrogen. In some embodiments, A is -C(O)-, R4 is other than hydrogen and R5 and R6 are hydrogen. In some embodiments, A is -C(O)-, R6 is other than hydrogen and R4 and R5 are hydrogen.

[0146] The compounds of Formula II, and all sub-embodiments detailed herein, may be used to treat a subject suffering from or at risk for any of the protein kinase mediated diseases or conditions contemplated herein.

[0147] In some embodiments, compounds of Formula II have the structure according to the following sub-generic structure Formula IIa:

A
R5 R12 NHS(O)2R24 Formula IIa all salts, prodrugs, tautomers and isomers thereof, wherein A, R4, R5, R6, R12, R16 and R24 are as defined for Formula II, provided, however, that when A is C(O), R4, R5, R6, and R12 are hydrogen and R16 is fluoro, then R24 is not CH3.

[0148] In some embodiments of compounds of Formula IIa, A is -CH2- or -C(O)-.

[0149] In other embodiments of compounds of Formula IIa, A is -CH2- or -C(O)-and R12 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio; in further embodiments, R12 and R16 are independently hydrogen, halogen, optionally fluoro substituted C1_3alkyl, optionally fluoro substituted C1_3alkoxy, or optionally fluoro substituted C1_3alkylthio. In some embodiments, A is -CH2- or -C(O)-, and R12 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio; in further embodiments, R12 and R16 are independently halogen, optionally fluoro substituted C1.3alkyl, optionally fluoro substituted C1_3alkoxy, or optionally fluoro substituted C1_3alkylthio.

[0150] In other embodiments of compounds of Formula IIa, A is -CH2-, R12 is hydrogen and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio; in further embodiments, R'6 is halogen, optionally fluoro substituted C1_3alkyl, optionally fluoro substituted C1_3alkoxy, or optionally fluoro substituted Cl_3alkylthio. In some embodiments, A is -CH2-, R16 is hydrogen and R12 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio; in further embodiments, R12 is halogen, optionally fluoro substituted C1_3alkyl, optionally fluoro substituted C1_3alkoxy, or optionally fluoro substituted CI.3alkylthio.

[0151] In some embodiments of compounds of Formula Ha, A is -CH2- and R12 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio; in further embodiments, R12 and R16 are independently halogen, optionally fluoro substituted C1_3alkyl, optionally fluoro substituted C1_3alkoxy, and optionally fluoro substituted C1_3alkylthio.

[0152] In other embodiments of compounds of Formula Ila, A is -C(O)-, R12 is hydrogen and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio; in further embodiments, R16 is halogen, optionally fluoro substituted C1_3alkyl, optionally fluoro substituted C1_3alkoxy, or optionally fluoro substituted C1_3alkylthio. In some embodiments, A is -C(O)-, R16 is hydrogen and R12 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio; in further embodiments, R12 is halogen, optionally fluoro substituted C1_3alkyl, optionally fluoro substituted C1_3alkoxy, or optionally fluoro substituted C1_3alkylthio.
[0153] In some embodiments of compounds of Formula Ila, A is -C(O)- and R12 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio; in further embodiments, R12 and R16 are independently hydrogen, halogen, optionally fluoro substituted C1_3alkyl, optionally fluoro substituted C1_3alkoxy, or optionally fluoro substituted C1_3alkylthio.

[0154] In some embodiments of any of the above embodiments of compounds of Formula Ha, R4 and R6 are hydrogen, or R5 and R6 are hydrogen, or R4 and R5 are hydrogen.

[0155] In some embodiments of any of the above embodiments of compounds of Formula Ila, R24 is substituted methyl, optionally substituted C2_6 alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl; in further embodiments, R24 is C2.6 lower alkyl, aryl or heteroaryl, wherein aryl and heteroaryl are optionally substituted with halogen, lower alkyl or lower alkoxy; in further embodiments, R24 is n-propyl, i-propyl, or phenyl, wherein phenyl is optionally substituted with halogen, lower alkyl or lower alkoxy.

[0156] In some embodiments, compounds of Formula II have the structure according to the following sub-generic structure Formula IIb:

A
::Hs(o)2NR28R29 Formula IIb all salts, prodrugs, tautomers and isomers thereof, wherein A, R4, R5, R6, R12, R14, R15 and R16, are as defined for Formula II, R28 is hydrogen, lower alkyl, or lower alkyl substituted with fluoro, hydroxyl, lower alkoxy, thiol, lower alkylthio, or -NR8R9, wherein R8 and R9 are as defined for Formula I, provided, however, that when R28 is substituted lower alkyl, any substitution of the alkyl carbon bound to the nitrogen of NR28 is fluoro, and R29 is hydrogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, provided, however, that nitrogen of NR29 is not bound to any alkene carbon thereof, optionally substituted lower alkynyl, provided, however, that nitrogen of NR29 is not bound to any alkyne carbon thereof, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R28 and R29 combine with the nitrogen to which they are attached form optionally substituted 5-7 membered heterocycloalkyl or optionally substituted 5 or 7 membered nitrogen containing heteroaryl. In some embodiments of compounds of Formula IIb, A is -CH2- or -C(O)-, R12, R14, R15, and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, R28 is hydrogen or lower alkyl and R29 is optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, or R28 and R29 combine with the nitrogen to which they are attached form optionally substituted 5-7 membered heterocycloalkyl or optionally substituted 5 or 7 membered nitrogen containing heteroaryl.

[0157] In some embodiments of compounds of Formula Ilb, A is -CH2-, one of R12, R14, R15 and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, R'4, R15 and R16 are hydrogen.
[0158] In other embodiments of compounds of Formula IIb, A is -CH2-, R12, R14 and R15 are hydrogen and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio.

[0159] In other embodiments of compounds of Formula fib, A is -CH2-, R14, R15 and R16 are hydrogen and R12 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio.

[0160] In some embodiments of compounds of Formula IIb, A is -CH2-, two of R12, R14, R15 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the other two of R12, R'4, R'5 and R16 are hydrogen.

[0161] In some embodiments of compounds of Formula IIb, A is -CH2-, R14 and R15 are hydrogen and R12 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio.

[0162] In some embodiments of compounds of Formula fib, A is -C(O)-, one of R12, R'4, R'5 and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the others of R12, R14, R'5 and R16 are hydrogen.
[0163] In some embodiments of compounds of Formula IIb, A is -C(O)-, R12, R14 and R15 are hydrogen and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio.

[0164] In some embodiments of compounds of Formula M, A is -C(O)-, R14, R'5 and R16 are hydrogen and R12 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio.

[0165] In some embodiments of compounds of Formula IIb, A is -C(O)-, two of R12, R14, R'5 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the other two of R12, R14, R15 and R16 are hydrogen.

[0166] In some embodiments of compounds of Formula fib, A is -C(O)-, R14and R15 are hydrogen and R12 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio.

[0167] In some embodiments of any of the above embodiments of compounds of Formula fib, R4 and R6 are hydrogen, or R5 and R6 are hydrogen, or R4 and R5 are hydrogen. In some embodiments of any of the above embodiments of compounds of Formula Ilb, R28 and R29 are both lower alkyl, or R28 and R29 combine with the nitrogen to which they are attached to form optionally substituted 5-7 membered heterocycloalkyl, further wherein the heterocycloalkyl is pyrrolidine, piperidine, piperazine or morpholine.

[0168] In some embodiments, compounds of Formula II have the structure according to the following sub-generic structures Formulae IIc, IId, Ile, IIf, or Iig:

R
4 q VN
12 HC(X)R24 Formula IIc \ 12 NHC(X)NHR24 /

Formula IId R 4 q 12 HC(X)OR24 Formula He R4 q VNNHS(0)2R R14 R

Formula IIf, or R4 r A
:iiiiiiiiiiii R12 2N"' N
H

Formula IIg all salts, prodrugs, tautomers and isomers thereof, wherein A, X, R4, R5, R6, R12, R14, R15, R16 and R24 are as defined for Formula II. In some embodiments of compounds of Formulae Ile, lid, Ile, llf, or IIg, A is -CH2- or -C(O)-. In some embodiments, any one of R12, R14, R15, and R16 is other than hydrogen. In some embodiments, any two of R12, R14, R1S, and R16 are other than hydrogen. In some embodiments, any three of R12, R14, R", and R16 are other than hydrogen. In some embodiments, R12, R14, R15, and R16 are other than hydrogen. In some embodiments, A is -CH2- or -C(O)- and R'2, R14, R15, and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -CH2-, any one of R12, R14, R'5 and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, R14, R15 and R16 are hydrogen. In some embodiments, A is -CH2-, R12, R14 and R15 are hydrogen and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -CH2-, R14, R15 and R16 are hydrogen and R12 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -CH2-, any two of R12, R14, R15 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining two of R'2, R14, R15 and R16 are hydrogen. In some embodiments, A is -CH2-, R14 and R15 are hydrogen and R12 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -C(O)-, any one of R12, R14, R15 and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, R14, R15 and R16 are hydrogen. In some embodiments, A is -C(O)-, R12, R14 and R'5 are hydrogen and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -C(O)-, R14, R'5 and R16 are hydrogen and R12 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -C(O)-, any two of R12, R14, R15 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally tuoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining two of R12, R14, R15 and R16 are hydrogen. In some embodiments, A is -C(O)-, R14 and R15 are hydrogen and R12 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments of any of the above embodiments of compounds of Formula Ilk, IId, Ile, IN, and IIg, R4 and R6 are hydrogen or R5 and R6 are hydrogen, or R4 and R5 are hydrogen.

[0169] In some embodiments, compounds of Formula II have the structure according to the following sub-generic structure Formula IIh:

A \ /
R

R
'' N
N Fi Formula IIh all salts, prodrugs, tautomers and isomers thereof, wherein A, R5, R12, R13, R15, R16 and R24 are as defined for Formula If, and Z is -0-, -S- or -NH-. In some embodiments of compounds of Formula IIh, R24 is hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, R24 is hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl and at least one of R12, R13, R15 and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the others of R12, R13, R15 and R 16 are hydrogen; in further embodiments, R13 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and R12, R15 and R16 are hydrogen; in further embodiments, A
is -CH2- or -C(O)-.
[0170] In some embodiments of compounds of Formula IIh, Z is -0- or -NH-, R24 is hydrogen, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, or lower alkyl optionally substituted with optionally substituted aryl or optionally substituted heteroaryl, and at least one of R12, R13, R15 and R16 is halogen, optionally fluoro substituted lower alkyl, or optionally fluoro substituted lower alkoxy and the others of R12, R13, R15 and R16 are hydrogen; in further embodiments, R13 is halogen, optionally fluoro substituted lower alkyl, or optionally fluoro substituted lower alkoxy and R12, R15 and R16 are hydrogen; in further embodiments, A is -CH2- or -C(O)-.

[0171] In some embodiments of compounds of Formula Il h, Z is -0- or -NH-, R24 is hydrogen, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, or lower alkyl optionally substituted with optionally substituted aryl or optionally substituted heteroaryl, R13 is halogen, optionally fluoro substituted lower alkyl, or optionally fluoro substituted lower alkoxy and one of R12, R15 and R16 is halogen, optionally fluoro substituted lower alkyl, or optionally fluoro substituted lower alkoxy and the others of R12, R15 and R16 are hydrogen; in further embodiments, A is -CH2- or -C(O)-.

[0172] In some embodiments of compounds of Formula Ilh, Z is -0- or NH-, R24 is hydrogen, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, or lower alkyl optionally substituted with optionally substituted aryl or optionally substituted heteroaryl, R13 is halogen, optionally fluoro substituted lower alkyl, or optionally fluoro substituted lower alkoxy and two of R12, R15 and R16 are independently halogen, optionally fluoro substituted lower alkyl, or optionally fluoro substituted lower alkoxy and the other of R12, R15 and R16 is hydrogen; in further embodiments, A is -CH2- or -C(O)-.

[0173] In some embodiments of compounds of Formula IIh, Z is -0- or -NH-, R24 is hydrogen, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, or lower alkyl optionally substituted with optionally substituted aryl or optionally substituted heteroaryl, R12, R13, R15 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, or optionally fluoro substituted lower alkoxy; in further embodiments, A is -CH2- or -C(O)-.

[0174] In some embodiments, compounds of Formula II have the structure according to the following sub-generic structures Formulae Ili, IIj, Ilk, IIm, or uIn:

NHC(X)R24 A

I \

N H

Formula Ili R1s NHC(X)NHR24 A

R4 ::/R13 I N N

Formula IIj NHC(X)OR24 R A

I / \

Formula Ilk R1s NHS(O)2R24 :R13 Formula 11m, or R NHS(O)2NHR24 A

R N H

Formula Tin all salts, prodrugs, tautomers and isomers thereof, wherein A, X, R4, R5, R6, R12, R13, R15, R16 and R24 are as defined for Formula II. In some embodiments of compounds of Formulae ii, IIj, Ilk, IIm, or IIn, A is -CH2- or -C(O)-. In some embodiments, one of R12, R13, R15, and R16 is other than hydrogen.
In some embodiments, two of R12, R13, R15, and R16 are other than hydrogen. In one embodiment, three of R12, R13, R", and R16 are other than hydrogen. In some embodiments, R12, R13, R15, and R16 are other than hydrogen.

[0175] In some embodiments of compounds of Formulae Hi, Hj, IIk, IIm, or Hn, A
is -CH2- or -C(O)-, R12, R13, R15, and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -CH2-, any one of R12, R'3, R15 and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, R13, R15 and R16 are hydrogen. In some embodiments, A is -CH2-, R13, R15 and R16 are hydrogen and R12 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -CH2-, R12, R15 and R16 are hydrogen and R13 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -CH2-, any two of R12, R13, R15 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining two of R12, R13, R15 and R'6 are hydrogen. In some embodiments, A is -CH2-, R15 and R16 are hydrogen and R12 and R13 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -CH2-, R13 and R16 are hydrogen and R12 and R15 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -CH2-, R13 and R15 are hydrogen and R12 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -CH2-, R12 and R16 are hydrogen and R13 and R15 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio.

[0176] In some embodiments of compounds of Formulae Ili, Hj, Ilk, Hm, or Hn, A
is -C(O)-, any one of R12, R13, R15 and R16 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining of R12, R13, R15 and R16 are hydrogen. In some embodiments, A is -C(O)-, R13, R15 and R16 are hydrogen and R12 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -C(O)-, R12, R15 and R'6 are hydrogen and R13 is halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -C(O)-, two of R12, R13, R15 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and the remaining two of R12, R'3, R15 and R16 are hydrogen. In some embodiments, A is -C(O)-, R15 and R16 are hydrogen and R12 and R13 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -C(O)-, R13 and R16 are hydrogen and R12 and R15 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -C(O)-, R13 and R15 are hydrogen and R12 and R16 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments, A is -C(O)-, R12 and R1fi are hydrogen and R13 and R15 are independently halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio. In some embodiments of any of the above embodiments of compounds of Formula Ia, R4 and R6 are hydrogen or R5 and R6 are hydrogen, or R4 and R5 are hydrogen.

[0177] In some embodiments, compounds of Formula II have the structure according to the following sub-generic structure Formula IIo:

A
ZR

H

Formula Ho all salts, prodrugs, tautomers and isomers thereof, wherein A, R5, R12, R14, R16 and R24 are as defined for Formula II, where each R24 is selected independently, and each Z is independently -0-, -S- or -NH-. In some embodiments of compounds of Formula IIo, each R24 is independently hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl. In some embodiments, each R24 is independently hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl and R12, R14 and R16 are hydrogen.

[0178] In some embodiments of compounds of Formula no, each R24 is independently hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl and at least one of R12, R14 and R16 is hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the others of R12, R14 and R16 are hydrogen; in further embodiments, R12 is hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and R14 and R16 are hydrogen; in further embodiments, A is -CH2- or -C(0)-.

[0179] In some embodiments of compounds of Formula Ho, each R24 is independently hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl and at least two of R12, R14 and R'6 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio, and the other of R12, R14 and R16 is hydrogen; in further embodiments, R12 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and R14 is hydrogen;
in further embodiments, A is -CH2- or -C(O)-.

[0180] In some embodiments of compounds of Formula IIo, L is -0- or -NH-, each R24 is independently hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, or optionally substituted heteroaryl, R12 and R16 are independently hydrogen, halogen, optionally fluoro substituted lower alkyl, optionally fluoro substituted lower alkoxy, or optionally fluoro substituted lower alkylthio and R14 is hydrogen; in further embodiments, A is -CH2- or -C(O)-.

[0181] The compounds of Formulae IIa-11o, and all sub-embodiments detailed herein, may be used to treat a subject suffering from or at risk for any of the protein kinase mediated diseases or conditions contemplated herein.

[0182] In some embodiments, compounds of Formula I have the structure according to the following sub-generic structure Formula III:

Formula III
all salts, prodrugs, tautomers, and isomers thereof, wherein:
Q has a structure selected from the group consisting of <032 7 2 R32 O
R17 R17 //) S
o ~ a Z6=Z5 R16 R15 4=4 - Z / X 14 iN N O
Z2 82 - 34 12 _ //~0 N R R HN S
RS/

and R43 N N.

in which - indicates the attachment point of Q to A of Formula III;
Z2 is N or CR12; Z4 is N or CR14; Z5 is N or CR15; Z6 is N or CR16;
L2 is selected from the group consisting of -(CR10R1)p NR25_(CR1 R11)q , -(CR10R11 0- CR10R11 CR10R11 S-(CR10R11 CR10R11 C(O)_(CR10R11) -(CR10R11)PC(S)-(CR10R1)q', _(CR1 R11)PS(O)-(CR10R11)9 , '(CR1 R11)P'S(O)2-(CR1 R1)q , -(CR10R11) _C(O)NR25-(CR10R11) _, _(CR10R11) C(S)NR25-(CR10R11) P q P` q, -(CR10R11)p S(O)2NR25_(CR'OR'')q, -(CR10R11)p NR25C(O)-(CR10R11)q-, -(CR10R11)P_NRa5C(S)_(CR10R1)q , and -(CR'OR'')P NR25S(O)2-(CR10R11)q ;

p and q are independently 0, 1, or 2 provided, however, that at least one of p and q is 0;
s is 1 or 2;
Xis 0 or S;
A is selected from the group consisting of -0-, -S-, -CRaRb-, -NR'-, -C(O)-, -C(S)-, -S(O)-, and -S(O)2-;
Ra and Rb at each occurrence are independently selected from the group consisting of hydrogen, fluoro, -OH, -NH2, lower alkyl, lower alkoxy, lower alkylthio, mono-alkylamino, di-alkylamino, and -NR8R9, wherein the alkyl chain(s) of lower alkyl, lower alkoxy, lower alkylthio, mono-alkylamino, or di-alkylamino are optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl chain carbon bound to 0 of alkoxy, S of thioalkyl or N of mono- or di-alkylamino is fluoro; or Ra and Rb combine to form a 3-7 membered monocyclic cycloalkyl or 5-7 membered monocyclic heterocycloalkyl, wherein the monocyclic cycloalkyl or monocyclic heterocycloalkyl are optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
R' is selected from the group consisting of hydrogen, lower alkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, -C(O)R7, -C(S)R7, -S(O)2R7, -C(O)NHR', -C(S)NHR7, and -S(O)2NHR7, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, lower alkylthio, mono-alkylamino, di-alkylamino, and -NR8R9, wherein the alkyl chain(s) of lower alkoxy, lower alkylthio, mono-alkylamino, or di-alkylamino are optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl chain carbon bound to 0 of alkoxy, S of thioalkyl or N of mono- or di-alkylamino is fluoro, further provided that when R' is lower alkyl, any substitution on the lower alkyl carbon bound to the N of -NR'- is fluoro, and wherein cycloalkyl, heterocycloalkyl, aryl or heteroaryl are optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
R7 is selected from the group consisting of lower alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, lower alkylthio, mono-alkylamino, di-alkylamino, and -NRSR9, provided, however, that any substitution of the alkyl carbon bound to the N of -C(O)NHR7, -C(S)NHR7 or -S(O)2NHR7 is fluoro, wherein the alkyl chain(s) of lower alkoxy, lower alkylthio, mono-alkylamino, or di-alkylamino are optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl chain carbon bound to 0 of alkoxy, S of thioalkyl or N of mono- or di-alkylamino is fluoro, and wherein cycloalkyl, heterocycloalkyl, aryl and heteroaryl are optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
Ra Rs R6 Rig R14 R15, R'6, R42, R43, R45R46 and R47 are independently selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -CN, -NO2, -CRaRbR26, and -LR26;
L at each occurrence is independently selected from the group consisting of -(alk)a-S-(alk)b-, -(alk)a-O-(alk)b-, -(alk)a-NR25-(alk)b-, -(alk)a-C(O)-(alk)b-, -(alk)a-C(S)-(alk)b-, -(alk)a-S(O)-(alk)b-, -(alk)a S(O)2-(alk)b-, -(alk)a-OC(O)-(alk)b-, -(alk)a C(O)O-(alk)b-, -(alk)a-OC(S)-(alk)b-, -(alk)a-C(S)O-(alk)b-, -(alk)a-C(O)NR2S-(alk)b-, -(alk)a C(S)NR25-(alk)b-, -(alk)a-S(O)2NR25-(alk)b-, -(alk)a-NR25C(O)-(alk)b-, -(alk)a NR25C(S)-(alk)b-, -(alk)a NR25S(O)2-(alk)b-, -(alk)a NR25C(O)O-(alk)b-, -(alk)a-NR25C(S)O-(alk)b-, -(alk)a-OC(O)NR25-(alk)b-, -(alk)a-OC(S)NR2S-(alk)b-, -(alk)a NR25C(O)NR25-(alk)b-, -(alk)a-NR25C(S)NR25-(alk)b-, and -(alk)a NR25S(O)2NR25-(alk)b-;
a and b are independently 0 or 1;
alk is C1_3 alkylene or C1_3 alkylene substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkyl, lower alkoxy, lower alkylthio, mono-alkylamino, di-alkylamino, and -NR8R9, wherein lower alkyl or the alkyl chain(s) of lower alkoxy, lower alkylthio, mono-alkylamino or di-alkylamino are optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution of the alkyl chain carbon bound to 0 of alkoxy, S of thioalkyl or N of mono- or di-alkylamino is fluoro;
R25 at each occurrence is independently selected from the group consisting of hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl;
R26 at each occurrence is independently selected from the group consisting of hydrogen, provided, however, that hydrogen is not bound to any of S(O), S(O)2i C(O) or C(S) of L, optionally substituted lower alkyl, optionally substituted lower alkenyl, provided, however, that when R26 is optionally substituted lower alkenyl, no alkene carbon thereof is bound to N, S, 0, S(O), S(O)2, C(O) or C(S) of L, optionally substituted lower alkynyl, provided, however, that when R26 is optionally substituted lower alkynyl, no alkyne carbon thereof is bound to N, S, 0, S(O), S(O)2, C(O) or C(S) of L, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl;
R10 and R11 at each occurrence are independently selected from the group consisting of hydrogen, fluoro, lower alkyl, and lower alkyl optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino; or any two of R10 and R11 on the same or adjacent carbon atoms combine to form a 3-7 membered monocyclic cycloalkyl or 5-7 membered monocyclic heterocycloalkyl, and any others of R10 and R' 1 are independently selected from the group consisting of hydrogen, fluoro, lower alkyl, and lower alkyl optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, and wherein the monocyclic cycloalkyl or monocyclic heterocycloalkyl are optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
R8 and R9 combine with the nitrogen to which they are attached to form a 5-7 membered heterocycloalkyl optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, and fluoro substituted lower alkylthio;
R17 is selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl and -OR18;
R31 and R33 are independently selected from the group consisting of optionally substituted aryl, optionally substituted heteroaryl, optionally substituted cycloalkyl, and optionally substituted heterocycloalkyl;
R36 is selected from the group consisting of substituted methyl, optionally substituted C2_6 alkyl, optionally substituted lower alkenyl, provided, however, that when R36 is optionally substituted lower alkenyl, no alkene carbon thereof is bound to the S(O)2 of S(O)2R36, optionally substituted lower alkynyl, provided, however, that when R36 is optionally substituted lower alkynyl, no alkyne carbon thereof is bound to the S(O)2 of S(O)2R36, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and -NR19R20;
R19, R20, R34, R35, R37, and R38 are independently selected from the group consisting of hydrogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, provided, however, that when R19, R20, R34, R35, R37, or R38 is optionally substituted lower alkenyl, no alkene carbon thereof is bound to the N of NR19R2o, NR34R35 or NR37R38, optionally substituted lower alkynyl, provided, however, that when R19, R2o, R34R3s R37, or R38 is optionally substituted lower alkynyl, no alkyne carbon thereof is bound to the N of NR19R20, NR34R35 or NR37R38, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl and optionally substituted heteroaryl; or R34 and R35 together with the nitrogen to which they are attached form optionally substituted 5-7 membered heterocycloalkyl or optionally substituted 5 or 7 membered nitrogen containing heteroaryl; or R37 and R38 together with the nitrogen to which they are attached form optionally substituted 5-7 membered heterocycloalkyl or optionally substituted 5 or 7 membered nitrogen containing heteroaryl;
R32 is selected from the group consisting of hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and -OR18;
R82 is selected from hydrogen or lower alkyl; and R18 is hydrogen or optionally substituted lower alkyl;
provided, however, that the compound is not 3-{3-[2-(tetrahydropyran-2-yloxy)-ethoxy]-benzyl}-5-thiophen-3-yl-lH-pyrrolo[2,3-b]pyridine, which has the structure S

H O
or 4-(4-Methyl-piperazin-l-ylmethyl)-N-[4-(1H-pyrrolo[2,3-b]pyridin-3-ylmethyl)-phenyl]-benzamide, which has the structure H
O ONE
N N
H
[0183] The compounds of Formula III, and all sub-embodiments detailed herein, maybe used to treat a subject suffering from or at risk for any of the protein kinase mediated diseases or conditions contemplated herein.

[0184] In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula IIIa:

[Z6 L R31 I

Formula IIIa all salts, prodrugs, tautomers and isomers thereof, wherein A, L2, Z2, Z6, R4, R5, R6, R15, R17 and R31 are as defined for Formula III.

[0185] In some embodiments of compounds of Formula IIIa, R4 and R6 are hydrogen, A is -0-, -CRaRb-, -NR'-, or -C(O)-, preferably -CH2- or -C(O)-, more preferably -CH2-, R17 is selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro, and R15 is selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy.

[0186] In some embodiments of compounds of Formula IIIa, R4 and R6 are hydrogen, A is -0-, -CRaRb-, -NR1-, or -C(O)-, preferably -CH2- or -C(O)-, R'7 is selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro, R15 is selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy, Z2 is N or CR12, Z6 is N or CR16, R12 and R16 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy, and R5 is selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and NR21R22, wherein R21 is hydrogen or lower alkyl, and R22 is hydrogen, lower alkyl, optionally substituted aryl or optionally substituted heteroaryl, and wherein the alkyl chain of R5, R21 or R22, when lower alkyl, or the alkyl chain of lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino.

[0187] In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula IM:

/Z16 L3-- Cy-(R23)t N N
H

Formula IIIb all salts, prodrugs, tautomers, and isomers thereof, wherein:
Al is -0-, -CR40R41-, -C(O)- or -NR48-;
Z12 is N or CR52;
Z16 is N or CR56;

R40 and R41 are independently selected from the group consisting of hydrogen, fluoro, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino; or R40 and R41 combine to form a 3-7 membered monocyclic cycloalkyl or 5-7 membered monocyclic heterocycloalkyl, wherein the monocyclic cycloalkyl or monocyclic heterocycloalkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
L3 is selected from the group consisting of -NR48-, -S-, -0-, -NR48CH(R49)-, -SCH(R49)-, -OCH(R49)-, -C(O)NR48-, -S(O)2NR48-, -CH(R49)NR48-, -CH(R49)O-, -CH(R49)S-, -NR48C(O)-, and -NR48S(O)2-;
R53 and R55 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with fluoro, -OH, NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino or cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro;
R52 and R56 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy;
R49 is selected from the group consisting of hydrogen, lower alkyl, and fluoro substituted lower alkyl;
Cy is selected from the group consisting of aryl, heteroaryl, cycloalkyl, and heterocycloalkyl;
R39 is selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, aryl, heteroaryl, and NR50R51, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, and wherein aryl and heteroaryl are optionally substituted with one or more independent substituents R23;
R50 is hydrogen or lower alkyl optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino;
R51 is aryl or heteroaryl, wherein aryl and heteroaryl are optionally substituted with one or more independent substituents R23;
R23 at each occurrence is independently selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -OR57, -SR57, -NR48R57, -NR48C(O)R57, -NR48S(O)2R57, -S(O)2R57, -C(O)R57, -C(O)OR57, -C(O)NR48R57, -S(O)2NR48R57, halogen, lower alkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl as R23, or as substituents of lower alkyl, are optionally substituted with one or more substituents selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2i -C(O)NH2, -OR58, -SR58, -NR48R58, -NR48C(O)R58 -NR48S(O)2R58, -S(O)2R58 -C(O)R58, -C(O)ORSS, -C(O)NR48R58, -S(O)2NR48R58, halogen, lower alkyl, fluoro substituted lower alkyl, and cycloalkylamino;
R57 is selected from the group consisting of lower alkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -OR57, -SR57, -NR48R57, -C(O)OR57, -C(O)NR48R57, or -S(O)2NR48R57 is fluoro, cycloalkyl, heterocycloalkyl, aryl or heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl as R57 or as substituents of lower alkyl are optionally substituted with one or more substituents selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -OR58, -SR58 W'W', -NR48C(O)R58, -NR48S(O)2R58, -S(O)2R58, _C(O)W8, -C(O)OR58, -C(O)NR48R5s, 'S(O)2NR48R5s, halogen, lower alkyl, fluoro substituted lower alkyl, and cycloalkylamino;
R58 at each occurrence is independently selected from the group consisting of lower alkyl, heterocycloalkyl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -OR58, -SR58, -NR48R58, -C(O)OR58, -C(O)NR48R58, or -S(O)2NR48R58 is fluoro;
R48 at each occurrence is independently hydrogen or lower alkyl; and t is 0, 1, 2, or 3.

[0188] In some embodiments of compounds of Formula IIIb, Al is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-, more preferably -CH2-. In some embodiments, A1 is -CR40R41-or -C(O)-, preferably -CH2- or -C(O)-, more preferably -CH2-, and R53 and R55 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy. In some embodiments, L3 is -NR48CH(R49)-, -SCH(R49)-, or -OCH(R49)-, preferably -OCH(R49)-. In some embodiments, Al is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-, more preferably -CH2-, and L3 is -NR48CH(R49)-, -SCH(R49)-, or -OCH(R49)-, preferably -OCH(R49)_ [0189] In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula IHp:

Z26- 4rCy-(R21)t A, N N
H

Formula HIp all salts, prodrugs, tautomers, and isomers thereof, wherein:
Al is -0-, -CR40R41-, -C(O)- or -NR48-;
Z22 is N or CR62;
Z26 is N or CR66;
r is 0, 1, or 2;
R40 and R41 are independently selected from the group consisting of hydrogen, fluoro, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino; or R40 and R41 combine to form a 3-7 membered monocyclic cycloalkyl or 5-7 membered monocyclic heterocycloalkyl, wherein the monocyclic cycloalkyl or monocyclic heterocycloalkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
R62, R63, R65 and R66 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro;
Cy is selected from the group consisting of aryl, heteroaryl, cycloalkyl, and heterocycloalkyl;
R39 is selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, aryl, heteroaryl, and NR50R51, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, and wherein aryl and heteroaryl are optionally substituted with one or more independent substituents R23;
R50 is hydrogen or lower alkyl optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino;
R51 is aryl or heteroaryl, wherein aryl and heteroaryl are optionally substituted with one or more independent substituents R23;
R23 at each occurrence is independently selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -ORS7, -SRS7, -NR48R57, -NR48C(O)R57, -NR48S(O) RS' S(O)2R57, -C(O)RS' C(O)ORS' C(O)NR48R57 S(O) NR48R57 halogen, lower alkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl as R23, or as substituents of lower alkyl, are optionally substituted with one or more substituents selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -OR58, -SR58, -NR48R58, NR48C(O)R58, NR48S(O)2R58, -S(O)2R58, -C(O)R58, -C(O)OR58, -C(O)NR48R58, -S(O)2NR48R58, halogen, lower alkyl, fluoro substituted lower alkyl, and cycloalkylamino;
R57 is selected from the group consisting of lower alkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -ORS7, -SR57, -NR48R57, -C(O)ORS7, -C(O)NR48R57, or -S(O)2NR48R57 is fluoro, cYcloal 1, heterocycloalkyl, or hetero 1, wherein cYcloal 1 ~' aryl ~'S' ~' , heterocycloalkyl, aryl, and heteroaryl as R57 or as substituents of lower alkyl are optionally substituted with one or more substituents selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -OR58, -SR", -NR48R58, -NRa8C(O)R58, -NR48S(O)2R58, -S(O)2RSS, -C(O)W', -C(O)OR58 -C(O)NI48R5s -S(O)ZNR 48RS8, halogen, lower alkyl, fluoro substituted lower alkyl, and cycloalkylamino;
R58 at each occurrence is independently selected from the group consisting of lower alkyl, heterocycloalkyl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -OR58, -SR58, -NR48R58, -C(O)OR58, -C(O)NR48R58, or -S(O)2NR48R58 is fluoro;
R48 at each occurrence is independently hydrogen or lower alkyl; and tis0, 1, 2, or 3.

[0190] In some embodiments of compounds of Formula IIIp, Al is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-. In some embodiments, Al is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-, and R62, R64, R65 and R66 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy.

[0191] In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula IlIc:

Zs sR32 :xz2RN N

Formula Me all salts, prodrugs, tautomers and isomers thereof, wherein A, s, Z2i Z6, R4, R5, R6, R15, R17, and R32 are as defined for Formula III.

[0192] In some embodiments of compounds of Formula IIIc, R4 and R6 are hydrogen, A is -0-, CRaRb-, -NR1-, or -C(O)-, preferably -CH2- or -C(O)-, more preferably -CH2-, R17 is selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro, and R15 is selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy.

[0193] In some embodiments of compounds of Formula IIIc, R4 and R6 are hydrogen, A is -0-, -CRaR''-, -NR'-, or -C(O)-, preferably -CH2- or -C(O)-, R17 is selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro, R15 is selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy, Z2 is N or CR12, Z6 is N or CR16, R12 and R16 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy, and R5 is selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and -NW 'R22, wherein R2' is hydrogen or lower alkyl, and R22 is hydrogen, lower alkyl, optionally substituted aryl or optionally substituted heteroaryl, and wherein the alkyl chain of R5, R21 or R22, when lower alkyl, or the alkyl chain of lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, further wherein R32 is optionally substituted lower alkyl or -OR18, where R18 is as defined for Formula III.

[0194] In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula IIIn:

Ra ) I
sR32 O~

Formula IIIn all salts, prodrugs, tautomers and isomers thereof, wherein A, s, Z2, Z6, R4, R5, R6, R15, and R32 are as defined for Formula III.

[0195] In some embodiments of compounds of Formula IRn, R4 and R6 are hydrogen, A is -0-, -CRaRb-, -NR1-, or -C(O)-, preferably -CH2- or -C(O)-, more preferably -CH2-, and R15 is selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy.

[0196] In some embodiments of compounds of Formula IIIc, R4 and R6 are hydrogen, A is -0-, -CRaRb-, -NR'-, or -C(O)-, preferably -CH2- or -C(O)-, R15 is selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy, Z2 is N or CR12, Z6 is N or CR16, R12 and R16 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy, and R5 is selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and -NR21R22, wherein R21 is hydrogen or lower alkyl, and R22 is hydrogen, lower alkyl, optionally substituted aryl or optionally substituted heteroaryl, and wherein the alkyl chain of R5, R21 or R22, when lower alkyl, or the alkyl chain of lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, further wherein R32 is optionally substituted lower alkyl or -OR18, where R18 is as defined for Formula III.

[0197] In some embodiments, compounds of Formula Ill have the structure according to the following sub-generic structure Formula Illo:

Z6%Z5 R4 ~Za ::xcz2L2_R33 N H

Formula Jib all salts, prodrugs, tautomers and isomers thereof, wherein A, L2, Z2, Z4, Z5, Z6, R4, R5, R6, and R33 are as defined for Formula III.

[0198] In some embodiments of compounds of Formula IIIo, R4 and R6 are hydrogen, A is -0-, -CRaRb-, -NR'-, or -C(O)-, preferably -CH2- or -C(O)-, Z2 is N or CR12, Z4 is N or CR14, Z5 is N or CR15, Z6 is N or CR16, and R12, R14, R15 and R16 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro.

[0199] In some embodiments of compounds of Formula IIIo, R4 and R6 are hydrogen, A is -0-, -Me-, -NR'-, or -C(O)-, preferably -CH2- or -C(O)-, Z2 is N or CR12, Z4 is N
or CR14, Z5 is N or CR15, Z6 is N or CR16, R12, R14, R15 and R16 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro, and R5 is selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and NR21R22, wherein R21 is hydrogen or lower alkyl, and R22 is hydrogen, lower alkyl, optionally substituted aryl or optionally substituted heteroaryl, and wherein the alkyl chain of R5, R21 or R22, when lower alkyl, or the alkyl chain of lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino.

[0200] In some embodiments of compounds of Formula IIIo, R4 and R6 are hydrogen, A is -0-, -Mae-, -NR1-, or -C(O)-, preferably -CH2- or -C(O)-, Z2 is N or CR12, Z4 is N
or CR14, Z5 is N or CR15, Z6 is N or CR16, R12, R14, R15 and R16 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy, and R5 is selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and NR21R22, Wherein R21 is hydrogen or lower alkyl, and R22 is hydrogen, lower alkyl, optionally substituted aryl or optionally substituted heteroaryl, and wherein the alkyl chain of R5, R21 or R22, when lower alkyl, or the alkyl chain of lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino.

[0201] In some embodiments, compounds of Formula fII have the structure according to the following sub-generic structure Formula lllq:

z16 Z15 A14 ,Z 14 L3--Cy-(R23)t N N
H

Formula IIIq all salts, prodrugs, tautomers, and isomers thereof, wherein:
Al is -0-, -CR40R41-, -C(O)- or -NR48-;
Z12 is N or CR52;
Z14 is N or CR54;
Z15 is N or CR55;
Z16 is N or CR56;

R40 and R4' are independently selected from the group consisting of hydrogen, fluoro, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino; or R40 and R4' combine to form a 3-7 membered monocyclic cycloalkyl or 5-7 membered monocyclic heterocycloalkyl, wherein the monocyclic cycloalkyl or monocyclic heterocycloalkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
L3 is selected from the group consisting of -NR48-, -S-, -0-, -NR48CH(R49)-, -SCH(R4)-, -OCH(R49)-, -C(O)NR48-, -S(O)2NR48-, -CH(R49)NR48-, -CH(R49)O-, -CH(R49)S-, -NR48C(O)-, and NR48S(O)2-;
R54 and R55 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with fluoro, -OH, NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino or cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro;
R52 and R56 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy;
R49 is selected from the group consisting of hydrogen, lower alkyl, and fluoro substituted lower alkyl;
Cy is selected from the group consisting of aryl, heteroaryl, cycloalkyl, and heterocycloalkyl;

R'y is selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, aryl, heteroaryl, and NR50R51, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, and wherein aryl and heteroaryl are optionally substituted with one or more independent substituents R23;
R50 is hydrogen or lower alkyl optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino;
R51 is aryl or heteroaryl, wherein aryl and heteroaryl are optionally substituted with one or more independent substituents R23;
R23 at each occurrence is independently selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2i -C(O)NH2, -ORS', -SRS7, -NR48R57, -NR48C(O)R57, -NR48S(O)2R57, -S(O)2R57, -C(O)R57, -C(O)OR57, -C(O)NR48R57, -S(O)Z NR 48 R57, halogen, lower alkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl as R23, or as substituents of lower alkyl, are optionally substituted with one or more substituents selected from the group consisting of -OH, -NH2i -CN, -NO2, -C(O)OH, -S(O)2NH2i -C(O)NH2, -OR58, -SR58, -NR48R58, -NR48C(O)R58, -NR48S(O)2R58, -S(O)2R58, -C(O)R58, -C(O)OR58, -C(O)NR48R58, -S(O)2NR48R58, halogen, lower alkyl, fluoro substituted lower alkyl, and cycloalkylamino;
R57 is selected from the group consisting of lower alkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -OR57, -SR57, -NR48R57, -C(O)OR57, -C(O)NR48R57, or -S(O)2NR48R57 is fluoro, cycloalkyl, heterocycloalkyl, aryl or heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl as R57 or as substituents of lower alkyl are optionally substituted with one or more substituents selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -OR58, -SR58, -NR48R58, -NR48C(O)R58, -NR48S(O)2R58, -S(O)2R58, -C(O)R58, -C(O)OR58, -C(O)NR48R58, -S(O)2NR48R58, halogen, lower alkyl, fluoro substituted lower alkyl, and cycloalkylamino;

R58 at each occurrence is independently selected from the group consisting of lower alkyl, heterocycloalkyl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -OR58, -SR58, -NR48R58, -C(O)OR58, -C(O)NR48R58, or -S(O)2NR48R58 is fluoro;
R48 at each occurrence is independently hydrogen or lower alkyl; and tis0, 1,2,or3.

[02021 In some embodiments of compounds of Formula IIIq, Al is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-, more preferably -CH2-. In some embodiments, AI is -CR40R41-or -C(O)-, preferably -CH2- or -C(O)-, more preferably -CH2-, and R54 and R55 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy. In some embodiments, L3 is -NR48CH(R49)-, -SCH(R49)-, or -OCH(R49)-, preferably -OCH(R49)-. In some embodiments, AI is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-, more preferably -CH2-, and L3 is -NR48CH(R49)-, -SCH(R49)-, or -OCH(R49)-, preferably -OCH(R49)_ [02031 In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula IIId:

Z6=Z5 A~ 4 :)tz20 \ \ Rss N r H
Formula Md.
all salts, prodrugs, tautomers and isomers thereof, wherein A, Z2, Z4, Z5, Z6, R4, R5, R6, R' , Ru and R33 are as defined for Formula 111, and r is 0, 1, or 2.

[02041 In some embodiments of compounds of Formula Illd, R4 and R6 are hydrogen, A is -0-, -CRV-, -NR'-, or -C(O)-, preferably -CH2- or -C(O)-, Z2 is N or CR12, Z4 is N
or CR14, Z5 is N or CR15, Z6 is N or CR16, and R12, R'4, R'5 and R16 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro.

[0205] In some embodiments of compounds of Formula IRd, R4 and R6 are hydrogen, A is -0-, -CRaRb-, -NR1-, or -C(O)-, preferably -CH2- or -C(O)-, Z2 is N or CR12, Z4 is N or CR14, Z 5 is N or CRIS, Z6 is N or CR16, R12, R14, Rls and R16 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2i lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro, R10 and R11 are independently selected from the group consisting of hydrogen, fluoro, lower alkyl, and fluoro substituted lower alkyl, and Rs is selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, optionally substituted aryl, 1, optionally substituted heteroaryl, and NR21R22 wherein R21 is hydrogen or lower alkyl, and R22 is hydrogen, lower alkyl, optionally substituted aryl or optionally substituted heteroaryl, and wherein the alkyl chain of Rs, R21 or R22, when lower alkyl, or the alkyl chain of lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino.
[0206] In some embodiments of compounds of Formula IIId, R4 and R6 are hydrogen, A is -0-, -CRaRb-, -NR1-, or -C(O)-, preferably -CH2- or -C(O)-, Z2 is N or CR12, Z4 is N or CR14, Z5 is N or CRIS, Z6 is N or CR16, R12, R14, Rls and R16 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy, R10 and R'1 are independently selected from the group consisting of hydrogen, fluoro, lower alkyl, and fluoro substituted lower alkyl, and Rs is selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and NR21R22, wherein R21 is hydrogen or lower alkyl, and R22 is hydrogen, lower alkyl, optionally substituted aryl or optionally substituted heteroaryl, and wherein the alkyl chain of Rs, R21 or R22, when lower alkyl, or the alkyl chain of lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino.

[0207] In some embodiments, compounds of Formula f have the structure according to the following sub-generic structure Formula IIIe:

Z26 Z25 %

\2_(_,cY_(R23)t A, R39 N N

H
Formula Me all salts, prodrugs, tautomers, and isomers thereof, wherein:
Al is -0-, -CR40R41-, -C(O)- or -NR48 ;
Z22 is N or CR62;

Z24 is N or CR64;
Z25 is N or CR65;
Z26 is N or CR66;
r is 0, 1, or 2;
R40 and R41 are independently selected from the group consisting of hydrogen, fluoro, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino; or R40 and R41 combine to form a 3-7 membered monocyclic cycloalkyl or 5-7 membered monocyclic heterocycloalkyl, wherein the monocyclic cycloalkyl or monocyclic heterocycloalkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
R6a, R64, R65 and R66 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro;
Cy is selected from the group consisting of aryl, heteroaryl, cycloalkyl, and heterocycloalkyl;
R39 is selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, aryl, heteroaryl, and NR50R51, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH,, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, and wherein aryl and heteroaryl are optionally substituted with one or more independent substituents R23;

R50 is hydrogen or lower alkyl optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino;
R51 is aryl or heteroaryl, wherein aryl and heteroaryl are optionally substituted with one or more independent substituents R23;
R23 at each occurrence is independently selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -ORS7, -SR57, -NR48R57, -NI48C(O)R57, -NR48S(O)2R57, -S(O)2R57, -C(O)R57, -C(O)OR57, -C(O)NR48R57, -S(O)2NR48R57, halogen, lower alkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl as R23, or as substituents of lower alkyl, are optionally substituted with one or more substituents selected from the group consisting of -OH, -NH2, -CN, NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -OR58, -SR58, -NR48R58, -NR48C(O)R58, -NR48S(O)2R58, -S(O)2R58, -C(O)R58, -C(O)OR", -C(O)NR48R58, -S(O)2NR48R58, halogen, lower alkyl, fluoro substituted lower alkyl, and cycloalkylamino;
R57 is selected from the group consisting of lower alkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -OR57, -SR57, -NR48R57, -C(O)OR57, -C(O)NR48R57, or -S(O)2NR48RS7 is fluoro, cycloalkyl, heterocycloalkyl, aryl or heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl as R57 or as substituents of lower alkyl are optionally substituted with one or more substituents selected from the group consisting of -OH, -NH2i -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -OR58, -SR58, -NR48R58, -NR48C(O)R58, -NR48S(O)2R58, -S(O)2R58, -C(O)R58, -C(O)OR58, -C(O)NR48R58, -S(O)2NR4sR58, halogen, lower alkyl, fluoro substituted lower alkyl, and cycloalkylamino;
R58 at each occurrence is independently selected from the group consisting of lower alkyl, heterocycloalkyl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -OR58, -SR58, -NR48R58, -C(O)OR58, -C(O)NR48R58, or -S(O)2NR48R58 is fluoro;

R48 at each occurrence is independently hydrogen or lower alkyl; and t is 0, 1, 2, or 3.

[0208] In some embodiments of compounds of Formula Me, A, is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-. In some embodiments, A, is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-, and R62, R64, R65 and R66 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy.
[0209] In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula IIIf:
Z
Z6=

R5 Z2~
N' N, R34 Formula IIIf all salts, prodrugs, tautomers and isomers thereof, wherein A, Z2, Z4, Z5, Z6, X, R4, R5, R6, R34, R35 and R82 are as defined for Formula III.

[0210] In some embodiments of compounds of Formula IIIf, R4 and R6 are hydrogen, A is -0-, -CRaRb-, -NR'-, or -C(O)-, preferably -CH2- or -C(O)-, Z2 is N or CR72, Z4 is N or CR14, Z5 is N or CR15, Z6 is N or CR16, and R12, R14, R15 and R16 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro.

[0211] In some embodiments of compounds of Formula IIIf; R4 and R6 are hydrogen, A is -0-, -CRaRb-, -NR'-, or -C(O)-, preferably -CH2- or -C(O)-, Z2 is N or CR12, Z4 is N or CR14, Z5 is N or CR15, Z6 is N or CR16, R12, R14, R15 and R'6 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro, and one of R34 and R35 is selected from the group consisting of optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl and optionally substituted heteroaryl, and the other of R34 and R35 is hydrogen or lower alkyl, or R34 and R35 together with the nitrogen to which they are attached form optionally substituted 5-7 membered heterocycloalkyl or optionally substituted 5 or 7 membered nitrogen containing heteroaryl.

[0212] In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula IIIg:

~/
R67 Z3a \
N 'R61 R82 %1 N N Rho H
Formula IIIg all salts, prodrugs, tautomers, and isomers thereof, wherein:
AI is -0-, -CR40R41-, -C(O)- or NR48-;
Z32 is N or CR72;
Z34 is N or CR74;
Z35 is N or CR75;
Z36 is N or CR76;
XisOorS;
R48 at each occurrence is independently hydrogen or lower alkyl;
R40 and R41 are independently selected from the group consisting of hydrogen, fluoro, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino; or R40 and R41 combine to form a 3-7 membered monocyclic cycloalkyl or 5-7 membered monocyclic heterocycloalkyl, wherein the monocyclic cycloalkyl or monocyclic heterocycloalkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
R72, R74, R75 and R76 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl and lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro;

R67 is selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -C(S)NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)OR68, -C(O)NR69R68, -C(S)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, -NR69C(S)R68a -NR69S(O)2 R68a - \ NR69C(O)NH2a -NR6 9C(O)NR69R68a -NR6 9C(S) 2a -NR69C(S)NR69R68, -NR69S(O)2NH2, -NR69S(O)2NR69R68, -S(O)R68, and -S(O)2R68;

one of R60 and R61 is lower alkyl, fluoro substituted lower alkyl, or -(CH2)0_2R70, and the other of R60 and R61 is hydrogen or lower alkyl;
or R60 and R61 together with the nitrogen to which they are attached form optionally substituted 5-7 membered heterocycloalkyl or optionally substituted 5 or 7 membered nitrogen containing heteroaryl;
R68 is selected from the group consisting of optionally substituted lower alkyl, optionally substituted lower alkenyl, provided, however, that when R68 is optionally substituted lower alkenyl, no alkene carbon thereof is bound to N, S, 0, S(O), S(O)2, C(O) or C(S) of -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)OR68, -C(O)NR69R68, -C(S)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, NR69C(S)R68a NR69S(O)2R68a NR69C(O)NH2, -NR69C(O)NR69R68, -NR69C(S)NH2, -NR69C(S)NR69R68, NR69S(O)2NH2, -NR69S(O)2NR69R68, -S(O)R68, or -S(O)2R68, optionally substituted lower alkynyl, provided, however, that when R68 is optionally substituted lower alkynyl, no alkyne carbon thereof is bound to Na S, 0, S(O), S(O)2, C(O) or C(S) of -OR6sa -SR6aa -NR69R6sa -C(O)W', -C(S)W', -C(O)OR68 C(O)NR69R68 C(S)NR69R68 S(O)2NR69R68 NR69C(O)R68 NR69C(S)R68 -NR69S(0)2R68, -NR69C(O)NH2, -NR69C(O)NR69R68, -NR69C(S)NH2, -NR69C(S)NR69R68, -NR69S(O)2NH2, -NR69S(O)2NR69R68, -S(O)R68, or -S(O)2R68, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl;
R69 is hydrogen or optionally substituted lower alkyl;
R70 is selected from the group consisting of optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl;
and R82 is hydrogen or lower alkyl.

[02131 In some embodiments of compounds of Formula IIIg, Al is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-.

[02141 In some embodiments of compounds of Formula IIIg, Al is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-, and R67 is selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -CN, -S(O)2NH2, -C(O)NH2, -OR68, -SR6s, -NR69R6s, -C(O)R6', -C(S)R68, -C(O)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, -NR69S(O)2R68, -S(O)R68, and -S(O)2R61 [02151 In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula IIIh:

I
R4 N _<N, R38 I \ R43 R42 Formula 11 h all salts, rodru s tautomers and isomers thereof, wherein A, X, R4, R5, R6, R37, R38R42, Rai R45, R46, and R47 are as defined for Formula III.

[02161 In some embodiments of compounds of Formula 1TIh, R4 and R6 are hydrogen, A is -0-, -CRaRb-, -NR'-, or -C(O)-, preferably -CH2- or -C(O)-, and R42, R43, R45, R46 and R47 are independently selected from the group consisting of hydrogen, halogen, -OH, -CN, -NO2, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkyl, mono-alkylamino, di-alkylamino, and cycloalkylamino, further wherein at least one of, at least two of, at least three of, at least four of, or preferably all of R42, R43, R45, R46 and R47 are hydrogen.

[0217] In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula IIIi:

N_ R60 Al N-<

N N
H
Formula HE
all salts, prodrugs, tautomers, and isomers thereof, wherein:

Al is -0-, -CR40R41-, -C(O)- or -NW'-,-X is0orS;
R48 at each occurrence is independently hydrogen or lower alkyl;
R40 and R41 are independently selected from the group consisting of hydrogen, fluoro, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino; or R40 and R41 combine to form a 3-7 membered monocyclic cycloalkyl or 5-7 membered monocyclic heterocycloalkyl, wherein the monocyclic cycloalkyl or monocyclic heterocycloalkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
R67 is selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -C(S)NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(0)2NH2, -OR68, -SR68, -NR69R68, `C(O)R68, -C(S)R", C(O)OR68 C(O)NR69R68 C(S)NR69R68 S(O)2NR69R68 -NR 69C(O) W ', -NR69C(S)R68, -NR69S(O)2R68, -NR69C(O)NH2, -NR69C(O)NR69R68, -NR69C(S)N 2i -NR69C(S)NR69R68, -NR69S(O)2NH2, -NR69S(O)2NR69R68, -S(O)R68, and -S(O)2R68;
one of R60 and R61 is lower alkyl, fluoro substituted lower alkyl, or -(CH2)0_2R70 and the other of R60 and R6' is hydrogen or lower alkyl;
or R60 and R61 together with the nitrogen to which they are attached form optionally substituted 5-7 membered heterocycloalkyl or optionally substituted 5 or 7 membered nitrogen containing heteroaryl;
R68 is selected from the group consisting of optionally substituted lower alkyl, optionally substituted lower alkenyl, provided, however, that when R68 is optionally substituted lower alkenyl, no alkene carbon thereof is bound to N, S, 0, S(O), S(O)2, C(O) or C(S) of -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)OR68, -C(O)NR69R68, -C(S)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, -NR69C(S)R68' NR69S(O)2R68, -N/R69C(O)NH2, -NR69C(O)NR69R68, -NR 69C(S)NH2, NR69C(S)NR69R68, -NR69S(O)2NH2, -NR69S(O)2NR69R68, -S(O)R68, or -S(O)2R68, optionally substituted lower alkynyl, provided, however, that when R68 is optionally substituted lower alkynyl, no alkyne carbon thereof is bound to N, S, 0, S(O), S(0)2, C(O) or C(S) of -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)O/R68, -C(O)NR69R68, -C(S)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, NR69C(S)R68, W'S(O)2R68, -NR69C(O)NH2i -NR69C(O)NR69R68, -NR69C(S)NH2, -NR69C(S)NR69R68, -NR69S(0)2NH2, -NR69S(0)2NR69R68, -S(O)R68, or -S(0)2R68, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl;
R69 is hydrogen or optionally substituted lower alkyl; and R70 is selected from the group consisting of optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl.
[02181 In some embodiments of compounds of Formula HE, Al is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-.

[0219] In some embodiments of compounds of Formula IlIi, Al is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-, and R67 is selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -CN, -S(O)2NH2, -C(O)NH2, -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, -NR69S(O)2R68, -S(O)R68, and -S(O)2R61 [0220] In some embodiments of compounds of Formula IIIi, Al is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-, more preferably -C(O)-, R67 is selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and NR21R22, wherein R21 is hydrogen or lower alkyl, and R22 is hydrogen, lower alkyl, optionally substituted aryl or optionally substituted heteroaryl, and wherein the alkyl chain of R67, R21 or R22, when lower alkyl, or the alkyl chain of lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, and one of R60 and R61 is lower alkyl or fluoro substituted lower alkyl, and the other of R60 and R61 is hydrogen or lower alkyl. In some embodiments, Al is -C(O)-, R67 is optionally substituted aryl or optionally substituted heteroaryl, and one of R60 and R61 is lower alkyl or fluoro substituted lower alkyl, and the other of R60 and R61 is hydrogen or lower alkyl.

[02211 In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula IIIj :

:R14 16 R4 ::x$ HN-AO

R2 Formula IIIj all salts, rodru s tautomers and isomers thereof, wherein A, R4, RS R6, R12 R'a R15 R16 and R36 are as defined for Formula III.

[02221 In some embodiments of compounds of Formula IIlj, R4 and R6 are hydrogen, A is -0-, -CRaRb-, -NR'-, or -C(O)-, preferably -CH2- or -C(O)-, and R'2, R14, R15 and R16 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro, preferably wherein R14 and R15 are hydrogen, more preferably wherein R12 is fluoro, R16 is hydrogen, fluoro or chloro, and R14 and R15 are hydrogen.

[02231 In some embodiments of compounds of Formula IIIj, R4 and R6 are hydrogen, A is -0-, -CRaRb-, -NR'-, or -C(O)-, preferably -CH2- or -C(O)-, R12, R14, R'5 and R16 are independently selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, wherein the alkyl chain of lower alkyl or lower alkoxy is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino and cycloalkylamino, provided, however, that any substitution on the alkyl carbon bound to the -0- of lower alkoxy is fluoro, preferably wherein R14 and R15 are hydrogen, more preferably wherein R12 is fluoro, R16 is hydrogen, fluoro or chloro, and R14 and R15 are hydrogen, and R36 is selected from the group consisting of optionally substituted C2_6 alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and -NR19R20, where R19 and R20 are as defined for Formula III, further wherein one of R19 and R20 is hydrogen or optionally substituted lower alkyl, and the other of R19 and R20 is selected from the group consisting of hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl and optionally substituted heteroaryl.

[02241 In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula 111k:

R71 Al R77 R81 N,S_ R78 H ~O
N N
H
Formula IIIk all salts, prodrugs, tautomers and isomers thereof, wherein:
Al is -0-, -CR40R41-, -C(O)- or -NR48-;

R81 is selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -OH, NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -C(S)NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)OR68, -C(O)NR69R68, -C(S)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, -NR69C(S)R68, -NR69S(O)2R68, -NR69C(O)NH2, -NR69C(0)NR69R68, -NR69C(S)NH2, -NR69C(S)NR69R68, -NR69S(0)2NH2, -NR69S(0)2NR69R68, -S(O)R68, and -S(O)2R68;
R71 and R78 are independently selected from the group consisting of hydrogen, halogen, C1_3 alkyl, and fluoro substituted C1_3 alkyl;
R77 is selected from the group consisting of substituted methyl, optionally substituted C2_6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, and -NR79R80, wherein methyl is substituted with one or more substituents selected from the group consisting of optionally substituted aryl and optionally substituted heteroaryl;
R68 is selected from the group consisting of optionally substituted lower alkyl, optionally substituted lower alkenyl, provided, however, that when R68 is optionally substituted lower alkenyl, no alkene carbon thereof is bound to N, S, 0, S(O), S(O)2, C(O) or C(S) of -OR68, -SR 68 NR69R68, -C(O)R68 C(S)R68 C(O)OR68 C(O)NR69R68 C(S)NR69R68 -S(O)2NR69R68' -NR69C(O)R68, -NR69C(S)R68, -NR69S(O)2R68' NR69C(O)NH2i -NR69C(O)NR69R68, -NR69C(S)NH2, -NR69C(S)NR69R68, -NR 69S(0)2N-H29 -NR69S(0)2NR69R68, -S(O)R68, or -S(O)2R68, optionally substituted lower alkynyl, provided, however, that when R68 is optionally substituted lower alkynyl, no alkyne carbon thereof is bound to N, S, 0, S(O), S(O)2, C(O) or C(S) of -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)OR68, -C(O)NR69R68, -C(S)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, NR69C(S)R68, -NR69S(O)2R68, -NR69C(O)NH2i -NR69C(O)NR69R68, -NR69C(S)NH2, -NR69C(S)NR69R68, -NR69s C N 1 69 69R68 68 ( )z z, -NRS(O)2NR , -S(O)W', or -S(0)2R68, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl;
R69 is hydrogen or optionally substituted lower alkyl; and R79 and R80 are independently hydrogen or optionally substituted lower alkyl, or R79 and R80 combine with the nitrogen to which they are attached to form optionally substituted 5-7 membered heterocycloalkyl.

[0225] In some embodiments of compounds of Formula iTik, Al is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-, more preferably -C(O)-.

[0226] In some embodiments of compounds of Formula IIIk, Al is -CR40R41- or -C(O)-, preferably -CH2- or -C(O)-, more preferably -C(O)-, and R8' is selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -CN, -S(O)2NH2, -C(O)NH2, -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, -NR69S(O)2R68, -S(O)R68, and -S(O)2R68.

[0227] In some embodiments, compounds of Formula III have the structure according to the following sub-generic structure Formula IIIm:

O O
Ral \S\R112 F H O
N N
H
Formula IIIm all salts, prodrugs, tautomers and isomers thereof, wherein:
R81 is selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -C(S)NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)OR68, -C(O)NR69R68, -C(S)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, -NR69C(S)R68, -NR69S(O)2R680 -NR69C(O)NH20 -NR69C(O)NR69R68, -NR69C(S)NH
l / 2a -NR69C(S)NR69R68, -NR69S(O)2NH2, -NR69S(O)2NR69R68, -S(O)R68, and -S(O)2R68;
R83 is selected from the group consisting of hydrogen, fluoro and chloro;
R112 is selected from the group consisting of optionally substituted C2_6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, and -NR79R80;
R68 is selected from the group consisting of optionally substituted lower alkyl, optionally substituted lower alkenyl, provided, however, that when R68 is optionally substituted lower alkenyl, no alkene carbon thereof is bound to N, S, 0, S(O), S(O)2, C(O) or C(S) of -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)OR", -C(O)NR69R68, -C(S)NR69R68, -S(O)2NR69R68, -NR6 9C(O)R68, -NR69C(S)R68, -NR6 9S(0)2R68, -NR 69C(O) NH2, -NR69C(O)NR69R68, -NR69C(S)NH2i -NR69C(S)NR69R68, -NR69S(O)2NH2, -NR69S(O)2NR69R6s, -S(O)R68, or -S(O)2R68, optionally substituted lower alkynyl, provided, however, that when R68 is optionally substituted lower alkynyl, no alkyne carbon thereof is bound to N, S, 0, S(O), S(O)z, C(O) or C(S) of -OR", -SR6s, -NR69R61, -C(O)R
61, -C(S)R61 , -C(O)OR68, -C(O)NR69R68, -C(S)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, -NR69C(S)R68, -NR69S(O)2R6s, -NR69C(O)NH2i -NR69C(O)NR69R68, -NR69C(S)NH2, -NR69C(S)NR69R68, -NR69S(O)2NH2, -NR69S(O)2NR69R68 )R68 68 -S(O)W', or -S(0) 2R , optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl;
R69 is selected from the group consisting of hydrogen and optionally substituted lower alkyl; and R79 and R80 are independently hydrogen or optionally substituted lower alkyl, or R79 and R80 combine with the nitrogen to which they are attached to form optionally substituted 5-7 membered heterocycloalkyl.

[02281 In some embodiments of compounds of Formula IIIm, R81 is selected from the group consisting of hydrogen, halogen, optionally substituted lower alkyl, optionally substituted lower alkenyl, optionally substituted lower alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -CN, -S(O)2NH2a -C(O)NH2i -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)NR69R68, -S(O)2NR69R6s, -NR69C(O)R6s, -NR69S(O)2R6s, -S(O)R68, and -S(O)2R6s [02291 The compounds of Formulae IIIa-IIIq, and all sub-embodiments detailed herein, may be used to treat a subject suffering from or at risk for any of the protein kinase mediated diseases or conditions contemplated herein.

[02301 In one aspect, the present invention includes compounds that are useful as intermediates in the preparation of compounds of Formula III, the compounds having a structure selected from the group consisting of Formula IV, Formula V, Formula VI, Formula VII, Formula VIII, and Formula IX
as follows:

R15 R15 Z6= Z5 Z6- Z6- R32 R108\ 'Z4 R108 Z 7 L2-R31 R1os-<\ p Z2 2 \ 2 s L2_ R33 Formula IV Formula V Formula VI

Z6= Z5 R15 N-R85 R43 NuN.R38 Z2 / R32 s Formula VII Formula VIII and Formula IX

wherein:
Z2, Z4, Z5, Z6, L2, X, S, R15, R17, R31, R32, R33, R37, R38, R42, R43, R45, R46, and R47 are as defined for Formula III;
R108 is selected from the group consisting of -C(O)R84, -CH2I, -CH2C1, -CH2Br, -CH2OH, and -CH2OS(O)2R109;
R109 is selected from the group consisting of lower alkyl and aryl;
R84 is selected from the group consisting of hydrogen, lower alkoxy, -OH, and -Cl;
R85 is selected from the group consisting of hydrogen, a nitrogen protecting group, -S(O)2R87, -C(O)NR88R89, and -C(S)NR88R89;
R86 is selected from the group consisting of hydrogen, lower alkyl, and a nitrogen protecting group;
R87 is selected from the group consisting of optionally substituted lower alkyl, optionally substituted lower alkenyl, provided, however, that when R87 is optionally substituted lower alkenyl, no alkene carbon thereof is bound to S(O)2, optionally substituted lower alkynyl, provided, however, that when R87 is optionally substituted lower alkynyl, no alkyne carbon thereof is bound to S(O)2i optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, and NR90R91;
and R88, R89, R90 and R91 are independently selected from the group consisting of hydrogen, optionally substituted lower alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl and optionally substituted heteroaryl; or R88 and R89 together with the nitrogen to which they are attached form optionally substituted 5-7 membered heterocycloalkyl or optionally substituted 5 or 7 membered nitrogen containing heteroaryl.

[0231] In some embodiments of compounds of Formulae IV, V, VI, VII, or VIII, R108 is -C(O)R84, preferably wherein R84 is hydrogen. In some embodiments of compounds of Formulae IV, V, VI, VII, or VIII, Z2 is N or CR12, Z4 is N or CR14, Z5 is N or CR15, and Z6 is N or CR16 and R12, R14, R15, R16, R17, R42, R43, R45, R46 and R47 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy.

[0232] In some embodiments, compounds of Formula IV have the structure according to the following sub-generic structure Formula Na:

L4-Cy-(R97).
O

Formula Na wherein:
R84 is selected from the group consisting of hydrogen, lower alkoxy, -OH, and -Cl;
R92, R93, R95, and R96 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy;
L4 is selected from the group consisting of -NR48-, -S-, -0-, -NR48CH(R49)-, -SCH(R49)-, -OCH(R49)-, -C(O)NR48-, -S(O)2NR48-, -CH(R49)NR48-, -CH(R49)O-, -CH(R49)S-, -NR48C(O)-, and NR48S(O)2-;
Cy is selected from the group consisting of cycloalkyl, heterocycloalkyl, aryl and heteroaryl;
R97 at each occurrence is independently selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2i -C(O)NH2, -OR57, -SR57, _NR48R57, _NR48C(O)R57, -NR48S O R57 S(O)2R57 C(O)R57 C(O)OR57 C(O)NR48R57 S(O) NR48R57 halogen, lower alkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl as R97, or as substituents of lower alkyl, are optionally substituted with one or more substituents selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -00, -SW', -NR48R58, -NR48C(O)R58, -NR48S(O)2R58, -S(O)2R58, -C(O)R58, -C(O)OR58, -C(O)NR48R58, -S(O)2NR48R58, halogen, lower alkyl, fluoro substituted lower alkyl, and cycloalkylamino;
R49 is selected from the group consisting of hydrogen, lower alkyl, and fluoro substituted lower alkyl;

R57 is selected from the group consisting of lower alkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -OR 57, -SR57, -NR4sR57, -C(O)OR 57, -C(O)NR4'R 17, or -S(O)2NR4sR57 is fluoro, cycloalkyl, heterocycloalkyl, aryl or heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl as R57 or as substituents of lower alkyl are optionally substituted with one or more substituents selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -OR58, -SR58, -NR48Rss, -NR4sC(O)Rss, -NR48S(O)2R58, -S(O)2R58, -C(O)R58, -C(O)OR58, -C(O)NR4sR5s, -S(O)ZNR 4s R5s, halogen, lower alkyl, fluoro substituted lower alkyl, and cycloalkylamino; and R58 at each occurrence is independently selected from the group consisting of lower alkyl, heterocycloalkyl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -OR58, -SR58, -NR48R58, -C(O)OR58, -C(O)NW IR58, or -S(0)2NR48R58 is fluoro;
R48 at each occurrence is independently hydrogen or lower alkyl; and u is 0, 1, 2, or 3.

[0233] In one embodiment of compounds of Formula IVa, at least two of R92, R93, R95 and R96 are hydrogen. In one embodiment, at least two of R92, R93, R95 and R96 are hydrogen, L4 is -NR48CH(R49)-, -SCH(R49)-, or -OCH(R49)-, preferably L4 is -OCH2-, Cy is aryl or heteroaryl, and each R97 is independently selected from the group consisting of halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy.

[0234] In some embodiments, compounds of Formula IV have the structure according to the following sub-generic structure Formula IVb:

Cy-(R97)u 0 r Formula IVb wherein R84, R92, R93, R95, R96, R97, Cy and u are as defined for Formula IVa and r is 0, 1 or 2.

[0235] In some embodiments of compounds of Formula IVb, at least two of R92, R93, R95 and R96 are hydrogen. In some embodiments, at least two of R92, R93, R95 and R96 are hydrogen, Cy is aryl or heteroaryl, and each R97 is independently selected from the group consisting of halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy.

[0236] In some embodiments, compounds of Formula V have the structure according to the following sub-generic structure Formula Va:

Os O

Formula Va wherein:
R84 is selected from the group consisting of hydrogen, lower alkoxy, -OH, and -Cl;
R92, R93, R95, and R96 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy;
R98 is selected from the group consisting of hydrogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy; and s is 0, 1, or 2;

[0237] In some embodiments, compounds of Formula VI have the structure according to the following sub-generic structure Formula Vla:

Rao R92 ~4_CY-(R97)u Formula VIa wherein:
R84 is selected from the group consisting of hydrogen, lower alkoxy, -OH, and -Cl;
R92, R94, R95, and R96 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy;
L4 is selected from the group consisting of -NR48-, -S-, -0-, -NR48CH(R49)-, -SCH(R49)-, -OCH(R49)-, -C(O)NR48-, -S(O)2NR48-, -CH(R49)NR48-, -CH(R49)O-, -CH(R49)S-, -NR48C(O)-, and -NR48S(O)2-;
Cy is selected from the group consisting of cycloalkyl, heterocycloalkyl, aryl and heteroaryl;

R97 at each occurrence is independently selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -OR57, -SRS7, -NR48R57, _NR48C(O)R57, -NR48S(O)2R57, -S(O)2R57, -C(O)R57, -C(O)OR57, -C(O)NR48R57, -S(O)2NR48R57, halogen, lower alkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl as R97, or as substituents of lower alkyl, are optionally substituted with one or more substituents selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -OR58, -SR58, -NR48R58 _NR48C(O)R58 -NR48S(O)2R58, -S(O)ZR58, -C(O)R58' -C(O)OR 58' -C(O)NR
48R58' -S(O)2NR48R58, halogen, lower alkyl, fluoro substituted lower alkyl, and cycloalkylamino;
R49 is selected from the group consisting of hydrogen, lower alkyl, and fluoro substituted lower alkyl;
R57 is selected from the group consisting of lower alkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -ORS' SRS' NR48R57 -C(O)OR 57, -C(O)NR4'R 17, or -S(O)2NR48R57 is fluoro, cycloalkyl, heterocycloalkyl, aryl or heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl as R57 or as substituents of lower alkyl are optionally substituted with one or more substituents selected from the group consisting of -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2i -C(O)NH2, -OR58, -SR58, -NR48R58, NR48C(O)R58, -NR48S(O)2R58, -S(O)2R58, -C(O)R58, -C(O)OR58, -C(O)NR48R58, -S(O)2NR48R58, halogen, lower alkyl, fluoro substituted lower alkyl, and cycloalkylamino;
R58 at each occurrence is independently selected from the group consisting of lower alkyl, heterocycloalkyl and heteroaryl, wherein lower alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -OR58, _SR58, _NR48R58, -C(O)OR58, -C(O)NR48RS8, or -S(O)2NR48R58 is fluoro;
R48 at each occurrence is independently hydrogen or lower alkyl; and uis0,1,2or3.

[0238] In some embodiments of compounds of Formula VIa, at least two of R92, R94, R95 and R96 are hydrogen. In some embodiments, at least two of R92, R94, R95 and R96 are hydrogen, L4 is -NR48CH(R49)-, -SCH(R49)-, or -OCH(R49)-, preferably L4 is -OCH2-, Cy is aryl or heteroaryl, and each R97 is independently selected from the group consisting of halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy.

[0239] In some embodiments, compounds of Formula VI have the structure according to the following sub-generic structure Formula VIb:

Rs4 \ I R94 O / Cy-(R97)u R92 O-(-!r Formula VIb wherein R84, R92, R94, R95, R96, R97, Cy and u are as defined for Formula VIa and r is 0, 1 or 2.
[0240] In some embodiments of compounds of Formula VIb, at least two of R92, R94, R95 and R96 are hydrogen. In some embodiments, at least two of R92, R94, R95 and R96 are hydrogen, Cy is aryl or heteroaryl, and each R97 is independently selected from the group consisting of halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy.

[0241] In some embodiments, compounds of Formula VII have the structure according to the following sub-generic structure Formula VIIa:

Rs4 O O
R92 HN-S//' O

Formula Vila wherein:
R84 is selected from the group consisting of hydrogen, lower alkoxy, -OH, and -Cl;
R92, R94, R95, and R96 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy;
R99 is selected from the group consisting of optionally substituted lower alkyl, optionally substituted aryl, optionally substituted heteroaryl, and -NR79R80; and R79 and R80 are independently hydrogen or optionally substituted lower alkyl, or R79 and R80 combine with the nitrogen to which they are attached to form optionally substituted 5-7 membered heterocycloalkyl.

[0242] In some embodiments of compounds of Formula Vila, one of R92 and R96 is selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy, and the other of R92 and R96 is selected from the group consisting of halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy; in further embodiments, one of R92 and R96 is hydrogen, fluoro or chloro, and the other of R92 and R96 is fluoro or chloro; in further embodiments, R92 is fluoro and R96 is hydrogen, fluoro, or chloro; in further embodiments, R92 and R96 are both fluoro.

[0243] In some embodiments of compounds of Formula Vila, R94 and R95 are hydrogen; in further embodiments, one of R92 and R96 is selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy and the other of R92 and R96 is selected from the group consisting of halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy; in further embodiments, one of R92 and R96 is selected from hydrogen, fluoro or chloro and the other of R92 and R96 is selected from fluoro or chloro; in further embodiments, R92 is fluoro and R96 is selected from hydrogen, fluoro, or chloro; in further embodiments, R92 and R96 are both fluoro.

[0244] In some embodiments, compounds of Formula VII have the structure according to the following sub-generic structure Formula VIIb:

Rs4 O X

R82 N-Roo Formula VIIb wherein:
Xis0orS;
R84 is selected from the group consisting of hydrogen, lower alkoxy, -O][1, and -Cl;
R92, R94, R95, and R96 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy;
R82 is hydrogen or lower alkyl;
one of R100 and R'0' is lower alkyl, fluoro substituted lower alkyl, or -(CH2)0.2R70, and the other of R100 and R101 is hydrogen or lower alkyl; or R10 and R10' together with the nitrogen to which they are attached form optionally substituted 5-7 membered heterocycloalkyl or optionally substituted 5 or 7 membered nitrogen containing heteroaryl; and R70 is selected from the group consisting of optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl.

[0245] In some embodiments of compounds of Formula VIIb, at least two of R92, R94, R95 and R96 are hydrogen. In some embodiments, at least two of R92, R94, R95 and R96 are hydrogen, and R70 is aryl or heteroaryl, wherein aryl and heteroaryl are optionally substituted with one or more substituents selected from the group consisting of halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy.

[0246] In some embodiments, compounds of Formula VIII have the structure according to the following sub-generic structure Formula VIIIa:

Rb07 R1 oa N N
`Rb 01 R1 02 x Formula VIIIa wherein:
Xis0orS;
R84 is selected from the group consisting of hydrogen, lower alkoxy, -OH, and -Cl;
R' 2, R' 3, 8105, R106, and R107 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy;
one of R10 and R101 is lower alkyl, fluoro substituted lower alkyl, or -(CH2)0_2R70 and the other of R100 and R101 is hydrogen or lower alkyl;
or R10 and R' 1 together with the nitrogen to which they are attached form optionally substituted 5-7 membered heterocycloalkyl or optionally substituted 5 or 7 membered nitrogen containing heteroaryl; and R70 is selected from the group consisting of optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl.
[0247] In some embodiments of compounds of Formula VIIIa, at least two, also at least three, also at least four, or all of R102, R' 3, R105, R106, and R107 are hydrogen. In some embodiments, at least two, also at least three, also at least four, or all of R' 2, R' 3, R105, R106, and R107 are hydrogen, and R70 is aryl or heteroaryl, wherein aryl and heteroaryl are optionally substituted with one or more substituents selected from the group consisting of halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy.

[0248] In some embodiments, compounds of Formula IX have the structure according to the following sub-generic structure Formula IXa:

Rao 0 qO Rsa RFo rmula IXa wherein:
R84 is selected from the group consisting of hydrogen, lower alkoxy, -OH, and -Cl;
R92, R95, and R96 are independently selected from the group consisting of hydrogen, halogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy;
R98 is selected from the group consisting of hydrogen, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, and fluoro substituted lower alkoxy; and s is 0, 1, or 2;

[0249] In some embodiments of any of the above embodiments of compounds of Formula IVa, IVb, Va, VIa, VIb, Vila, VIIb, VIIIa, or IXa, R84 is hydrogen.

[0250] In some embodiments of the above compounds, compounds are excluded where N (except where N is a heteroaryl ring atom), 0, or S is bound to a carbon that is also bound to N (except where N is a heteroaryl ring atom), 0, or S; or where N (except where N is a heteroaryl ring atom), 0, C(S), C(O), or S(O),, (n is 0-2) is bound to an alkene carbon of an alkenyl group or bound to an alkyne carbon of an alkynyl group; accordingly, in some embodiments compounds which include linkages such as the following are excluded from the present invention: -NR-CH2-NR-, -O-CH2-NR-, -S-CH2-NR-, -NR-CH2-O-, -O-CH2-O-, -S-CH2-O-, -NR-CH2-S-, -O-CH2-S-, -S-CH2-S-, -NR-CH=CH-, -CH=CH-NR-, -NR-C EC-, -C 2-NR-, -O-CH=CH-, -CH=CH-O-, -0-C ac-, -C EC-O-, -S(0)0_2-CH=CH-, -CH=CH-S(0)0_2-, -S(0)0_2-C MC-, -C EC-S(0)0_2-, -C(O)-CH=CH-, -CH=CH-C(O)-, -C aC-C(O)-, or -C(O)-C EC-, -C(S)-CH=CH-, -CH=CH-C(S)-, -C ~C-C(S)-, or -C(S)-C ac-.

[0251] In reference to compounds herein, specification of a compound or group of compounds includes pharmaceutically acceptable salts of such compound(s) unless clearly indicated to the contrary, prodrug(s), and all stereoisomers. In reference to compositions, kits, methods of use, etc. of compounds of Formula I described herein, it is understood that a compound of Formula I includes compounds of Formulae la-Iz, and all sub-embodiments thereof, compounds of Formula II, including Formulae Ila-IIo, and all sub-embodiments thereof, and compounds of Formula III, including Formulae HIa-IIIq, and all sub-embodiments thereof, unless indicated otherwise. In reference to compositions, kits, methods of use, etc. of compounds of Formula II described herein, it is understood that this includes compounds of Formulae IIa-IIo, and all sub-embodiments thereof, unless indicated otherwise. In reference to compositions, kits, methods of use, etc. of compounds of Formula III
described herein, it is understood that this includes compounds of Formulae Ma-IIIq, and all sub-embodiments thereof, unless indicated otherwise.

[0252] In one aspect, the invention provides methods for treating a protein kinase mediated disease or condition in an animal subject, wherein the method involves administering to the subject an effective amount of a compound of Formula I. The terms "treat," "therapy," and like terms refer to the administration of material, e.g., compound of Formula I, in an amount effective to prevent, alleviate, or ameliorate one or more symptoms of a disease or condition, i.e., indication, and/or to prolong the survival of the subject being treated. The term "protein kinase mediated disease or condition" refers to a disease or condition in which the biological function of a protein kinase affects the development and/or course of the disease or condition, and/or in which modulation of the protein kinase alters the development, course, and/or symptoms of the disease or condition. A protein kinase mediated disease or condition includes a disease or condition for which modulation provides a therapeutic benefit, e.g. wherein treatment with protein kinase inhibitors, including compounds described herein, provides a therapeutic benefit to the subject suffering from or at risk of the disease or condition. In one aspect, the method involves administering to the subject an effective amount of a compound of Formula I in combination with one or more other therapies for the disease or condition.
[0253] In one aspect, the invention provides methods for treating a protein kinase mediated disease or condition in an animal subject, wherein the method involves administering to the subject an effective amount of a compound of any one or more of Formula la through Formula Iz, and all sub-embodiments thereof.

[0254] In another aspect, the invention provides methods for treating a protein kinase mediated disease or condition in an animal subject, wherein the method involves administering to the subject an effective amount of a compound of Formula II.

[0255] In another aspect, the invention provides methods for treating a protein kinase mediated disease or condition in an animal subject, wherein the method involves administering to the subject an effective amount of a compound of any one or more of Formula IIa through Formula Ho, and all sub-embodiments thereof.

[0256] In another aspect, the invention provides methods for treating a protein kinase mediated disease or condition in an animal subject, wherein the method involves administering to the subject an effective amount of a compound of Formula III.

[0257] In another aspect, the invention provides methods for treating a protein kinase mediated disease or condition in an animal subject, wherein the method involves administering to the subject an effective amount of a compound of any one or more of Formula IIIa through Formula IIIo, and all sub-embodiments thereof.

[0258] In one aspect, the invention provides methods for treating a Raf protein kinase mediated disease or condition in an animal subject, wherein the method involves administering to the subject an effective amount of a compound of Formula I. The terms "Raf protein kinase mediated disease or condition," "Raf mediated disease or condition," and the like refer to a disease or condition in which the biological function of a Raf kinase, including any mutations thereof, affects the development and/or course of the disease or condition, and/or in which modulation of the Raf protein kinase alters the development, course, and/or symptoms of the disease or condition. The Raf protein kinase includes, but is not limited to, B-Raf, mutations of B-Raf, c-Raf-1 and mutations of c-Raf-1. In some embodiments, the Raf protein kinase is B-Raf mutation V600E. In further embodiments, the disease or condition is a cancer that is amenable to treatment by an inhibitor of the V600E mutant B-Raf. A
Raf protein kinase mediated disease or condition includes a disease or condition for which Raf inhibition provides a therapeutic benefit, e.g. wherein treatment with Raf inhibitors, including compounds described herein, provides a therapeutic benefit to the subject suffering from or at risk of the disease or condition. In one aspect, the method involves administering to the subject an effective amount of a compound of Formula I in combination with one or more other therapies for the disease or condition.

[0259] In one aspect, the invention provides methods for treating a Raf protein kinase mediated disease or condition in an animal subject, wherein the method involves administering to the subject an effective amount of a compound of Formula Ha or III. In one aspect, the method involves administering to the subject an effective amount of a compound of Formula IIa or III in combination with one or more other therapies for the disease, further wherein the compound is of Formula IIIj, 111k, or IIIm. The Raf protein kinase includes, but is not limited to, B-Raf, mutations of B-Raf, c-Raf-1 and mutations of c-Raf-1. In some embodiments, the Raf protein kinase is B-Raf mutation V600E.
In further embodiments, the disease or condition is a cancer that is amenable to treatment by an inhibitor of the V600E mutant B-Raf.

[0260] In one aspect, the invention provides methods for treating a Fms protein kinase mediated disease or condition in an animal subject, wherein the method involves administering to the subject an effective amount of a compound of Formula I. The terms "Fms protein kinase mediated disease or condition," "Fms mediated disease or condition," and the like refer to a disease or condition in which the biological function of a Fms protein kinase, including any mutations thereof, affects the development and/or course of the disease or condition, and/or in which modulation of Fms alters the development, course, and/or symptoms of the disease or condition. A Fms mediated disease or condition includes a disease or condition for which Fms inhibition provides a therapeutic benefit, e.g.
wherein treatment with Fms inhibitors, including compounds described herein, provides a therapeutic benefit to the subject suffering from or at risk of the disease or condition.
In one aspect, the method involves administering to the subject an effective amount of a compound of Formula I in combination with one or more other therapies for the disease or condition.

[0261] In one aspect, the invention provides methods for treating a Kit protein kinase mediated disease or condition in an animal subject, wherein the method involves administering to the subject an effective amount of a compound of Formula I. The terms "Kit mediated disease or condition," "Kit protein kinase mediated disease or condition," and the like refer to a disease or condition in which the biological function of a Kit protein kinase, including any mutation thereof, affects the development and/or course of the disease or condition, and/or in which modulation of Kit alters the development, course, and/or symptoms of the disease or condition. A Kit mediated disease or condition includes a disease or condition for which Kit inhibition provides a therapeutic benefit, e.g. wherein treatment with Kit inhibitors, including compounds described herein, provides a therapeutic benefit to the subject suffering from or at risk of the disease or condition. In one aspect, the method involves administering to the subject an effective amount of a compound of Formula I in combination with one or more other therapies for the disease or condition.

[0262] In one aspect, the invention provides methods for treating a Jnk protein kinase mediated disease or condition in an animal subject, wherein the method involves administering to the subject an effective amount of a compound of Formula I. The terms "Jnk mediated disease or condition," "Jnk protein kinase mediated disease or condition," and the like refer to a disease or condition in which the biological function of a Jnk kinase, e.g. Jnkl, Jnk2, Jnk3, or any mutation thereof, affects the development and/or course of the disease or condition, and/or in which modulation of the Jnk kinase alters the development, course, and/or symptoms of the disease or condition. A
Jnk mediated disease or condition includes a disease or condition for which Jnk inhibition provides a therapeutic benefit, e.g. wherein treatment with Jnk inhibitors, including compounds described herein, provides a therapeutic benefit to the subject suffering from or at risk of the disease or condition. In one aspect, the method involves administering to the subject an effective amount of a compound of Formula I in combination with one or more other therapies for the disease or condition. The Jnk protein kinase includes, but is not limited to, Jnkl, Jnk2, or Jnk3.

[0263] In some embodiments, a compound of Formula I will have an IC50 of less than 500 nm, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than 1 nM as determined in a generally accepted kinase activity assay. In some embodiments, a compound of any of Formula I will have an IC50 of less than 500 mu, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than 1 nM with respect to at least one kinase selected from the group consisting of Abl, Aktl, Akt2, Akt3, ALK, A1k5, B-Raf, Brk, Btk, Cdk2, CDK4, CDK5, CDK6, CHK1, c-Raf-1, Csk, EGFR, EphAl, EphA2, EphB2, EphB4, Erk2, Fak, FGFR1, FGFR2, FGFR3, FGFR4, Fltl, Flt3, Flt4, Fms, Frk, Fyn, Gsk3a, Gsk3(3, HCK, Her2/Erbb2, Her4/Erbb4, IGF1R, IKK beta, Irak4, Itk, Jakl, Jak2, Jak3, Jnkl, Jnk2, Jnk3, Kdr, Kit, LCK, MAP2K1, MAP2K2, MAP4K4, MAPKAPK2, Met, Mnkl, MLKI, p38, PDGFRA, PDGFRB, PDPK1, Piml, Pim2, Pim3, PKC alpha, PKC beta, PKC theta, Plkl, Pyk2, ROCK1, ROCK2, Ron, Src, Stk6, Syk, TEC, Tie2, TrkA, Yes, and Zap70, including any mutations thereof.

[0264] In some embodiments, a compound of Formula I will have an IC50 of less than 500 nm, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than 1 nM
with respect to at least one kinase selected from the group consisting of Abl, Akt 1, Akt2, Akt3, ALK, A1k5, B-Raf, Btk, Cdk2, CDK4, CDK5, CDK6, CHKI, c-Raf-1, Csk, EGFR, EphAl, EphA2, EphB2, EphB4, Erk2, Fak, Fms, Fyn, Gsk3a, Gsk3j3, HCK, Her2/Erbb2, Her4/Erbb4, IGF1R, IKK beta, Irak4, Itk, Jakl, Jak2, Jak3, Jnkl, Jnk2, Jnk3, Kit, LCK, MAP2K1, MAP2K2, MAP4K4, MAPKAPK2, Met, Mnkl, MLK1, p38, PDPK1, Piml, Pim2, Pim3, PKC alpha, PKC beta, PKC theta, Plkl, Pyk2, Ron, Src, Stk6, Syk, TEC, Tie2, TrkA, Yes, and Zap70, including any mutations thereof.

[0265] In some embodiments, a compound of Formula I will have an IC50 of less than 500 nm, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than 1 nM
with respect to at least one kinase selected from the group consisting of Abl, B-Raf, Btk, c-Raf-1, EGFR, EphB2, Erk2, Fak, FGFR1, Fltl, Flt3, Flt4, Fms, Irak4, Jnkl, Jnk2, Jnk3, Kdr, Kit, MAP2K1, MAPKAP kinase 2, Met, p38, PDGFRB, Piml, PKC theta, Pyk2, Ret, Src, Stk6, Yes, and Zap70, including any mutations thereof.

[0266] In some embodiments, a compound of Formula I will have an IC50 of less than 500 mu, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than 1 nM
with respect to at least one kinase selected from the group consisting of Abl, B-Raf, Btk, c-Raf-1, EGFR, EphB2, Erk2, Fak, Fms, Irak4, Jnkl, Jnk2, Jnk3, Kit, MAP2K1, MAPKAP
kinase 2, Met, p38, Piml, PKC theta, Pyk2, Src, Stk6, Yes, and Zap70, including any mutations thereof.

[0267] In some embodiments, a compound of Formula II will have an IC50 of less than 500 nm, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 riM, or less than 1 nM
with respect to at least one kinase selected from the group consisting of B-Raf, B-Raf V600E mutant, c-Raf-1, FGFR1, FGFR2, FGFR3, FGFR4, Jnkl, Jnk2, Jnk3, Met, Piml, Pim2, Pim3, Pyk2, Kdr and Ret, including any mutations thereof.

[0268] In some embodiments, a compound of Formula III will have an IC50 of less than 500 nm, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than I nM
with respect to at least one kinase selected from the group consisting of B-Raf, c-Raf-1, Fms, Jnkl, Jnk2, Jnk3, and Kit, and any mutations thereof. In some embodiments, a compound of any of Formula III will have an IC50 of less than 500 nm, less than 100 nM, less than 50 nM, less than 20 DM, less than 10 nM, less than 5 nM, or less than 1 nM with respect to at least one kinase selected from the group consisting of B-Raf, B-Raf V600E mutant, c-Raf-1, Fms, Jnkl, Jnk2, Jnk3, and Kit, preferably B-Raf, B-Raf V600E mutant or c-Raf-1.

[0269] In some embodiments, a compound of Formula III is an inhibitor of a Raf kinase and has an IC50 of less than 500 mu, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than 1 nM as determined in a generally accepted Raf kinase activity assay. In some embodiments, a compound of Formula III will have an IC50 of less than 500 nm, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than 1 nM with respect to B-Raf, c-Raf-1, or B-Raf V600E mutant. In some embodiments, a compound of Formula III will selectively inhibit one Raf kinase relative to one or more other Raf kinases.
In some embodiments, the compound of Formula III will selectively inhibit a mutation of the Raf kinase relative to the wild type kinase, for example B-Raf V600E relative to wild type B-Raf.

[0270] In some embodiments, a compound of Formula III is an inhibitor of a Fms kinase and has an IC50 of less than 500 rm1, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than 1 nM as determined in a generally accepted Fms kinase activity assay. In some embodiments, a compound of Formula III will selectively inhibit Fms kinase relative to Kit kinase.

[0271] In some embodiments, a compound of Formula III is an inhibitor of a Kit kinase and has an IC50 of less than 500 nm, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than 1 nM as determined in a generally accepted Kit kinase activity assay.

[0272] In some embodiments, a compound of Formula III is an inhibitor of a Jnk kinase and has an IC50 of less than 500 mn, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than 1 nM as determined in a generally accepted Jnk kinase activity assay. In some embodiments, a compound of Formula III is an inhibitor of a Jnkl kinase and has an IC50 of less than 500 mn, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 rM, less than 5 nM, or less than 1 nM as determined in a generally accepted Jnkl kinase activity assay. In some embodiments, a compound of Formula III is an inhibitor of a Jnk2 kinase and has an IC50 of less than 500 mn, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than 1 nM as determined in a generally accepted Jnk2 kinase activity assay. In some embodiments, a compound of Formula III is an inhibitor of a Jnk3 kinase and has an IC50 of less than 500 nm, less than 100 nM, less than 50 nM, less than 20 nM, less than 10 nM, less than 5 nM, or less than 1 nM as determined in a generally accepted Jnk3 kinase activity assay. In some embodiments, a compound of Formula III will selectively inhibit one Jnk kinase relative to one or more other Jnk kinases, such as selectively inhibiting Jnk 1 relative to Jnk 2 and/or Jnk3, selectively inhibiting Jnk2 relative to Jnk3 and/or Jnkl, or selectively inhibiting Jnk3 relative to Jnkl and/or Jnk 2.

[02731 Further to any of the above mentioned embodiments, a compound of the invention will also inhibit the effects of a mutation of the kinase, including, but not limited to, a mutation that is related to a disease state, such as a cancer. For example, B-Raf V600E mutant is present in a high percentage of some cancers, such as melanoma, and compounds of the invention will inhibit the kinase activity of this mutant.

[02741 Further to any of the above embodiments, a compound of the invention may selectively inhibit one kinase relative to one or more other kinases, where preferably inhibition is selective with respect to any of the other kinases, whether a kinase discussed herein, or other kinases. In some embodiments, the compound may selectively inhibit the effects of a mutation of the kinase relative to the wild type kinase, for example B-Raf V600E relative to wild type B-Raf. In some embodiments, the compound may selectively inhibit Fms relative to Kit. Selective inhibition of one kinase relative to another is such that the IC50 for the one kinase may be at least about 2-fold, also 5-fold, also 10-fold, also 20-fold, also 50-fold, or at least about 100-fold less than the IC50 for any of the other kinases as determined in a generally accepted kinase activity assay.

[02751 In another aspect, the invention provides compositions that include a therapeutically effective amount of a compound of Formula I and at least one pharmaceutically acceptable carrier, excipient, and/or diluent. The composition can include a plurality of different pharmacologically active compounds, which can include a plurality of compounds of Formula I. In another aspect, the composition can include one or more compounds of Formula I, Formula II, or Formula III along with one or more compounds that are therapeutically effective for the same disease indication. In one aspect, the composition includes one or more compounds of Formula I, Formula II, or Formula III
along with one or more compounds that are therapeutically effective for the same disease indication, wherein the compounds have a synergistic effect on the disease indication.

[02761 In another aspect, the invention provides compositions that include a therapeutically effective amount of at least one compound of Formula III and at least one pharmaceutically acceptable carrier, excipient, and/or diluent. The composition can include a plurality of different pharmacologically active compounds, which can include a plurality of compounds of Formula III, or can include at least one compound of Formula III along with at least one compound that is therapeutically effective for the same disease indication. In one aspect, the at least one compound of Formula III and the at least one compound that is therapeutically effective for the same disease indication have a synergistic effect on the disease indication. In one aspect, the composition includes one or more compounds of Formula III effective in treating a cancer and one or more other compounds that are effective in treating the cancer, further wherein the compounds are synergistically effective in treating the cancer.

[0277] In another aspect, the invention provides a method for modulating the activity of a protein kinase selected from the group consisting of Abl, Aktl, Akt2, Akt3, ALK, Alk5, B-Raf, Brk, Btk, Cdk2, CDK4, CDK5, CDK6, CHK1, c-Raf-l, Csk, EGFR, EphAl, EphA2, EphB2, EphB4, Erk2, Fak, FGFR1, FGFR2, FGFR3, FGFR4, Fltl, Flt3, Flt4, Fms, Frk, Fyn, Gsk3a, Gsk3(3, HCK, Her2/Erbb2, Her4/Erbb4, IGF1R, TKK beta, Irak4, Itk, Jakl, Jak2, Jak3, Jnkl, Jnk2, Jnk3, Kdr, Kit, LCK, MAP2K1, MAP2K2, MAP4K4, MAPKAPK2, Met, Mnkl, MLK1, p38, PDGFRA, PDGFRB, PDPK1, Pimi, Pim2, Pim3, PKC alpha, PKC beta, PKC theta, Plkl, Pyk2, ROCK1, ROCK2, Ron, Src, Stk6, Syk, TEC, Tie2, TrkA, Yes, or Zap70 by contacting the protein kinase with an effective amount of a compound of Formula I.

[0278] In another aspect, the invention provides methods for treating a protein kinase mediated disease or condition in an animal subject, wherein the method involves administering to the subject an effective amount of a composition including a compound of Formula I.

[0279] In one aspect, the invention provides methods for treating a disease or condition mediated by a protein kinase selected from the group consisting of Abl, Akt 1, Akt2, Akt3, ALK, Alk5, B-Raf, Btk, Cdk2, CDK4, CDK5, CDK6, CHK1, c-Raf-1, Csk, EGFR, EphAl, EphA2, EphB2, EphB4, Erk2, Fak, FGFR1, FGFR2, FGFR3, FGFR4, Fltl, Flt3, Flt4, Fms, Fyn, Gsk3a, Gsk3(3, HCK, Her2/Erbb2, Her4/Erbb4, IGF1R, IKK beta, Irak4, Itk, Jakl, Jak2, Jak3, Jnkl, Jnk2, Jnk3, Kdr, Kit, LCK, MAP2K1, MAP2K2, MAP4K4, MAPKAPK2, Met, Mnkl, MLK1, p38, PDGFRA, PDGFRB, PDPK1, Piml, Pim2, Pim3, PKC alpha, PKC beta, PKC theta, Plkl, Pyk2, ROCK1, ROCK2, Ron, Src, Stk6, Syk, TEC, Tie2, TrkA, Yes, and Zap70 by administering to the subject an effective amount of a composition including a compound of Formula I.

[0280] In one aspect, the invention provides methods for treating a disease or condition mediated by a protein kinase selected from the group consisting of Abl, Akt 1, Akt2, Akt3, ALK, Alk5, B-Raf, Btk, Cdk2, CDK4, CDK5, CDK6, CHK1, c-Raf-1, Csk, EGFR, EphAl, EphA2, EphB2, EphB4, Erk2, Fak, Fms, Fyn, Gsk3a, Gsk3f3, HCK, Her2/Erbb2, Her4/Erbb4, IGF1R, IKK
beta, Irak4, Itk, Jakl, Jak2, Jak3, Jnkl, Jnk2, Jnk3, Kit, LCK, MAP2K1, MAP2K2, MAP4K4, MAPKAPK2, Met, Mnkl, MLK1, p38, PDPK1, Piml, Pim2, Pim3, PKC alpha, PKC beta, PKC theta, Plkl, Pyk2, Ron, Src, Stk6, Syk, TEC, Tie2, TrkA, Yes, and Zap70 by administering to the subject an effective amount of a composition including a compound of Formula 1.

[0281] In one aspect, the invention provides methods for treating a disease or condition mediated by a protein kinase selected from the group consisting of Abl, B-Raf, Btk, c-Raf-1, EGFR, EphB2, Erk2, Fak, FGFR1, Fltl, F1t3, Flt4, Fms, Irak4, Jnkl, Jnk2, Jnk3, Kdr, Kit, MAP2K1, MAPKAPK2, Met, p38, PDGFRB, Piml, PKC theta, Pyk2, Ret, Src, Stk6, Yes, and Zap70 by administering to the subject an effective amount of a composition including a compound of Formula I.

[0282] In one aspect, the invention provides methods for treating a disease or condition mediated by a protein kinase selected from the group consisting of Abl, B-Raf, Btk, c-Raf-1, EGFR, EphB2, Erk2, Fak, Fms, Irak4, Jnkl, Jnk2, Jnk3, Kit, MAP2K1, MAPKAPK2, Met, p38, Piml, PKC theta, Pyk2, Src, Stk6, Yes, and Zap70 by administering to the subject an effective amount of a composition including a compound of Formula I.

[0283] In one aspect, the invention provides methods for treating a disease or condition mediated by a protein kinase selected from the group consisting of B-Raf, B-Raf V600E
mutant, c-Raf-1, FGFR1, FGFR2, FGFR3, FGFR4, Jnkl, Jnk2, Jnk3, Met, Piml, Pim2, Pim3, Pyk2, Kdr and Ret by administering to the subject an effective amount of a composition including a compound of Formula I.
[0284] In one aspect, the invention provides methods for treating a disease or condition mediated by a protein kinase selected from the group consisting of B-Raf, c-Raf-1, Fms, Jnkl, Jnk2, Jnk3, and Kit, and any mutations thereof, by administering to the subject an effective amount of a composition including a compound of Formula III.

[0285] In one aspect, the invention provides methods for treating a disease or condition mediated by B-Raf, c-Raf-1, or B-Raf V600E by administering to the subject an effective amount of a composition including a compound of Formula II or Formula III, where in further embodiments, the compound is of Formula ila or Formula III. In one aspect, the invention provides methods for treating a disease or condition mediated by B-Raf, c-Raf-1, or B-Raf V600E by administering to the subject an effective amount of a composition including a compound of Formula IIIj, IIIk, or IIim in combination with one or more other suitable therapies for treating the disease. In one aspect, the invention provides methods for treating a cancer mediated by B-Raf V600E mutant by administering to the subject an effective amount of a composition of Formula IIIj, IIIk, or IIIm in combination with one or more suitable anticancer therapies, such as one or more chemotherapeutic drugs.

[0286] In one aspect, the invention provides a method of treating a cancer by administering to the subject an effective amount of a composition including a compound of Formula I, or where the compound is of Formula III, or where the compound is of Formula IIIj, I111c, or IIim, in combination with one or more other therapies or medical procedures effective in treating the cancer. Other therapies or medical procedures include suitable anticancer therapy (e.g. drug therapy, vaccine therapy, gene therapy, photodynamic therapy) or medical procedure (e.g.
surgery, radiation treatment, hyperthermia heating, bone marrow or stem cell transplant). In one aspect, the one or more suitable anticancer therapies or medical procedures is selected from treatment with a chemotherapeutic agent (e.g. chemotherapeutic drug), radiation treatment (e.g. x-ray, y-ray, or electron, proton, neutron, or a particle beam), hypertliermia heating (e.g. microwave, ultrasound, radiofrequency ablation), Vaccine therapy (e.g. AFP gene hepatocellular carcinoma vaccine, AFP adenoviral vector vaccine, AG-858, allogeneic GM-CSF-secretion breast cancer vaccine, dendritic cell peptide vaccines), gene therapy (e.g. Ad5CMV-p53 vector, adenovector encoding MDA7, adenovirus 5-tumor necrosis factor alpha), photodynamic therapy (e.g. aminolevulinic acid, motexafin lutetium), surgery, and bone marrow and stem cell transplantation.

[02871 In a preferred embodiment, the invention provides a method of treating a cancer by administering to the subject an effective amount of a composition including a compound of Formula I, or wherein the compound is of Formula III, or wherein the compound is of Formula 111j, IM, or 111m, in combination with one or more suitable chemotherapeutic agents. In one aspect, the one or more suitable chemotherapeutic agents is selected from an alkylating agent, including, but not limited to, adozelesin, altretamine, bizelesin, busulfan, carboplatin, carboquone, cannustine, chlorambucil, cisplatin, cyclophosphamide, dacarbazine, estramustine, fotemustine, hepsulfam, ifosfamide, improsulfan, irofulven, lomustine, mechlorethamine, melphalan, oxaliplatin, piposulfan, semustine, streptozocin, temozolomide, thiotepa, and treosulfan; an antibiotic, including, but not limited to, bleomycin, dactinomycin, daunorubicin, doxorubicin, epirubicin, idarubicin, menogaril, mitomycin, mitoxantrone, neocarzinostatin, pentostatin, and plicamycin; an antimetabolite, including, but not limited to, azacitidine, capecitabine, cladribine, clofarabine, cytarabine, decitabine, floxuridine, fludarabine, 5-fluorouracil, ftorafur, gemcitabine, hydroxyurea, mercaptopurine, methotrexate, nelarabine, pemetrexed, raltitrexed, thioguanine, and trimetrexate; an immunotherapy, including, but not limited to, alemtuzumab, bevacizumab, cetuximab, galiximab, gemtuzumab, panitumumab, pertuzumab, rituximab, tositumomab, trastuzumab, and 90 Y ibritumomab tiuxetan; a hormone or hormone antagonist, including, but not limited to, anastrozole, androgens, buserelin, diethylstilbestrol, exemestane, flutamide, fulvestrant, goserelin, idoxifene, letrozole, leuprolide, magestrol, raloxifene, tamoxifen, and toremifene; a taxane, including, but not limited to, DJ-927, docetaxel, TPI 287, paclitaxel and DHA-paclitaxel; a retinoid, including, but not limited to, alitretinoin, bexarotene, fenretinide, isotretinoin, and tretinoin; an alkaloid, including, but not limited to, etoposide, homoharringtonine, teniposide, vinblastine, vincristine, vindesine, and vinorelbine; an antiangiogenic agent, including, but not limited to, AE-941 (GW786034, Neovastat), ABT-510, 2-methoxyestradiol, lenalidomide, and thalidomide; a topoisomerase inhibitor, including, but not limited to, amsacrine, edotecarin, exatecan, irinotecan (also active metabolite SN-38 (7-ethyl-10-hydroxy-camptothecin)), rubitecan, topotecan, and 9-aminocamptothecin; a kinase inhibitor, including, but not limited to, erlotinib, gefitinib, flavopiridol, imatinib mesylate, lapatinib, sorafenib, sunitinib malate, AEE-788, AG-013736, AMG 706, AMN107, BMS-354825, BMS-599626, UCN-01 (7-hydroxystaurosporine), and vatalanib; a targeted signal transduction inhibitor including, but not limited to bortezomib, geldanamycin, and rapamycin; a biological response modifier, including, but not limited to, imiquimod, interferon-a, and interleukin-2; and other chemotherapeutics, including, but not limited to 3-AP (3-amino-2-carboxyaldehyde thiosemicarbazone), aminoglutethimide, asparaginase, bryostatin-1, cilengitide, E7389, ixabepilone, procarbazine, sulindac, temsirolimus, tipifarnib. Preferably, the method of treating a cancer involves administering to the subject an effective amount of a composition of Formula I (more preferably Formula Ill, and even more preferably Formulae IIIj, 111k, or I im) in combination with a chemotherapeutic agent selected from 5-fluorouracil, carboplatin, dacarbazine, gefitinib, oxaliplatin, paclitaxel, SN-38, temozolomide, vinblastine, bevacizumab, cetuximab, or erlotinib.

[0288] In another aspect, the invention provides a method of treating or prophylaxis of a disease or condition in a mammal, by administering to the mammal a therapeutically effective amount of a compound of Formula I, a prodrug of such compound, or a pharmaceutically acceptable salt of such compound or prodrug. The compound can be alone or can be part of a composition. In another aspect, the invention provides a method of treating or prophylaxis of a disease or condition in a mammal, by administering to the mammal a therapeutically effective amount of a compound of Formula III, a prodrug of such compound, or a pharmaceutically acceptable salt of such compound or prodrug in combination with one or more other suitable therapies for the disease or condition.

[0289] In another aspect, the invention provides compositions that include a therapeutically effective amount of a compound of Formula III and at least one pharmaceutically acceptable carrier, excipient, and/or diluent. The composition can include a plurality of different pharmacologically active compounds, which can include a plurality of compounds of Formula III.

[0290] In a related aspect, the invention provides kits that include a composition as described herein. In some embodiments, the composition is packaged, e.g., in a vial, bottle, flask, which may be further packaged, e.g., within a box, envelope, or bag; the composition is approved by the U.S. Food and Drug Administration or similar regulatory agency for administration to a mammal, e.g., a human;
the composition is approved for administration to a mammal, e.g., a human, for a protein kinase mediated disease or condition; the invention kit includes written instructions for use and/or other indication that the composition is suitable or approved for administration to a mammal, e.g., a human, for a protein kinase-mediated disease or condition; and the composition is packaged in unit dose or single dose form, e.g., single dose pills, capsules, or the like.

[0291] In aspects involving treatment or prophylaxis of a disease or condition with the compounds of Formula I, the disease or condition is, for example without limitation, neurologic diseases such as ischemic stroke, cerebrovascular ischemia, multi-infarct dementia, head injury, spinal cord injury, Alzheimer's disease (AD), Parkinson's disease, amyotrophic lateral sclerosis, dementia, senile chorea, and Huntington's disease; neoplastic diseases and associated complications, including chemotherapy-induced hypoxia, gastrointestinal stromal tumors (GISTs), prostate tumors, mast cell tumors (including canine mast cell tumors), acute myeloid leukemia, acute lymphocytic leukemia, chronic myeloid leukemia, chronic lymphocytic leukemia, multiple myeloma, melanoma, mastocytosis, gliomas, glioblastoma, astrocytoma, neuroblastoma, sarcomas (e.g. sarcomas of neuroectodermal origin, leiomyosarcoma), carcinomas (e.g. lung, breast, pancreatic, colon, hepatocellular, renal, female genital tract, squamous cell, carcinoma in situ), lymphoma (e.g. histiocytic lymphoma, non-Hodgkin's lymphoma), MEN2 syndromes, neurofibromatosis (including Schwann cell neoplasia), myelodysplastic syndrome, leukemia, tumor angiogenesis, and cancers of the thyroid, liver, bone, skin, brain, central nervous system, pancreas, lung (e.g. small cell lung cancer, non small cell lung cancer), breast, colon, bladder, prostate, gastrointestinal tract, endometrium, fallopian tube, testes and ovary; pain of neuropathic or inflammatory origin, including acute pain, chronic pain, and migraine;
cardiovascular diseases including heart failure, cardiac hypertrophy, thrombosis (e.g. thrombotic microangiopathy syndromes), atherosclerosis, reperfusion injury and ischemia (e.g. cerebrovascular ischemia, liver ischemia); inflammation including, but not limited to, polycystic kidney disease (PKD), age-related macular degeneration, rheumatoid arthritis, allergic rhinitis, inflammatory bowel disease (1BD), ulcerative colitis, Crohn's disease, systemic lupus erythematosis, Sjogren's Syndrome, Wegener's granulomatosis, psoriasis, scleroderma, chronic thyroiditis, Grave's disease, myasthenia gravis, multiple sclerosis, osteoarthritis, endometriosis, scarring (e.g.
dermal, tissue), vascular restenosis, fibrotic disorders, hypereosinophilia, CNS inflammation, pancreatitis, nephritis, atopic dermatitis, and hepatitis; immunodeficiency diseases (e.g. severe combined immunodeficiency (SCID)), organ transplant rejection, graft versus host disease; renal or prostatic diseases including diabetic nephropathy, nephrosclerosis, glomerulonephritis, interstitial nephritis, Lupus nephritis, prostate hyperplasia, chronic renal failure, tubular necrosis, diabetes-associated renal complications, and hypertrophy; metabolic diseases including type 1 diabetes, type 2 diabetes, metabolic syndrome, obesity, hepatic steatosis, insulin resistance, hyperglycemia, lipolysis and obesity; infection, including, but not limited to Helicobacter pylori and Influenza virus, fever, sepsis; pulmonary diseases including chronic obstructive pulmonary disease (COPD), acute respiratory distress syndrome (ARDS), asthma, allergy, bronchitis, emphysema, and pulmonary fibrosis; genetic developmental diseases such as Noonan's syndrome, Crouzon syndrome, acrocephalo-syndactyly type I, Pfeiffer's syndrome, Jackson-Weiss syndrome, Costello syndrome, (faciocutaneoskeletal syndrome), leopard syndrome, cardio-faciocutaneous syndrome (CFC) and neural crest syndrome abnormalities causing cardiovascular, skeletal, intestinal, skin, hair and endocrine diseases; disorders of bone structure or mineralization, including osteoporosis, increased risk of fracture, hypercalcemia, and bone metastases; Grave's disease; Hirschsprung's disease; lymphoedema;
selective T-cell defect (STD); X-linked agammaglobulinemia; diabetic retinopathy; alopecia; erectile dysfunction; and tuberous sclerosis.

[0292] In a related aspect, compounds of Formula I, further where the compound is of Formula III, can be used in the preparation of a medicament for the treatment of a B-Raf-mediated disease or condition, selected from the group consisting of neurologic diseases such as ischemic stroke, multi-infarct dementia, head injury, spinal cord injury, Alzheimer's disease (AD), Parkinson's disease;
neoplastic diseases including, but not limited to, melanoma, glioma, sarcoma, carcinoma (e.g. lung, breast, pancreatic, renal), lymphoma (e.g. histiocytic lymphoma) and cancer of the thyroid, lung (e.g.
small cell lung cancer), liver, breast, ovary and colon, neurofibromatosis, myelodysplastic syndrome, leukemia, tumor angiogenesis; pain of neuropathic or inflammatory origin, including acute pain, chronic pain, and migraine; cardiovascular diseases including heart failure, cardiac hypertrophy, thrombosis (e.g. thrombotic microangiopathy syndromes), atherosclerosis, reperfusion injury;
inflammation including, but not limited to, psoriasis, polycystic kidney disease (PKD), arthritis and autoimmune diseases and conditions, osteoarthritis, endometriosis, scarring, vascular restenosis, fibrotic disorders, rheumatoid arthritis, inflammatory bowel disease (IBD);
immunodeficiency diseases, organ transplant rejection, graft versus host disease; renal or prostatic diseases including diabetic nephropathy, nephrosclerosis, glomerulonephritis, prostate hyperplasia; metabolic disorders, obesity; infection, including, but not limited to Helicobacter pylori and Influenza virus, fever, sepsis;
pulmonary diseases including chronic obstructive pulmonary disease (COPD) and acute respiratory distress syndrome (ARDS); genetic developmental diseases such as Noonan's syndrome, Costello syndrome, (faciocutaneoskeletal syndrome), leopard syndrome, cardio-faciocutaneous syndrome (CFC), and neural crest syndrome abnormalities causing cardiovascular, skeletal, intestinal, skin, hair and endocrine diseases.

[0293] In a related aspect, compounds of Formula III, further where the compound is of Formula 111, can be used in the preparation of a medicament for the treatment of a c-Raf-1 -mediated disease or condition selected from the group consisting of colorectal, ovarian, lung and renal cell carcinoma, acute myeloid leukemia, myelodysplastic syndromes, tumor angiogenesis, and neuroendocrine tumors such as medullary thyroid cancer, carcinoid, small cell lung cancer and pheochromocytoma.

[0294] In a related aspect, compounds of Formula III, further where the compound is of Formula III, can be used in the preparation of a medicament for the treatment of a Fins-mediated disease or condition selected from the group consisting of immune disorders, including rheumatoid arthritis, systemic lupus erythematosis (SLE), Wegener's granulomatosis, and transplant rejection, inflammatory diseases including Chronic Obstructive Pulmonary Disease (COPD), emphysema, and atherosclerosis, metabolic disorders, including insulin resistance, hyperglycemia, and lipolysis, disorders of bone structure or mineralization, including osteoporosis, increased risk of fracture, hypercalcemia, and bone metastases, kidney diseases, including nephritis (e.g.
glomerulonephritis, interstitial nephritis, Lupus nephritis), tubular necrosis, diabetes-associated renal complications, and hypertrophy and cancers, including multiple myeloma, acute myeloid leukemia, chronic myeloid leukemia (CML), breast cancer, and ovarian cancer.

[0295] In a related aspect, compounds of Formula III, further where the compound is of Formula III, can be used in the preparation of a medicament for the treatment of a Jnk-mediated disease or condition selected from the group consisting of Metabolic diseases including type 1 diabetes, type 2 diabetes, metabolic syndrome, obesity, and hepatic steatosis; cardiovascular diseases such as atherosclerosis, ischemia (e.g. cerebrovascular ischemia, liver ischemia), reperfusion injury, cardiac hypertrophy; renal diseases such as chronic renal failure; neoplastic diseases and associated complications, including chemotherapy-induced hypoxia, prostate tumors, myeloid leukemia and cancers of the liver, bone, skin, brain, pancreas, lung breast, colon, prostate and ovary; transplant rejection; pain of neuropathic or inflammatory origin including acute and chronic pain; inflammatory and autoimmune diseases including age-related macular degeneration, rheumatoid arthritis, inflammatory bowel disease, ulcerative colitis, Crohn's disease, systemic lupus erythematosis, Sj ogren's Syndrome, psoriasis, scleroderma, chronic thyroiditis, Grave's disease, myasthenia gravis, and multiple sclerosis, and inflammation in other organs including CNS
inflammation, pancreatitis, nephritis, atopic dermatitis, and hepatitis; airway inflammatory diseases such as asthma, allergy, bronchitis, pulmonary fibrosis, chronic obstructive pulmonary disease;
neurologic diseases such as stroke, cerebrovascular ischemia, neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis, dementia, senile chorea, head and spinal cord trauma, and Huntington's disease.

[0296] In a related aspect, compounds of Formula III, further where the compound is of Formula III, can be used in the preparation of a medicament for the treatment of a Jnkl -mediated disease or condition selected from the group consisting of type 1 diabetes, type 2 diabetes, metabolic syndrome, obesity and hepatic steatosis.

[0297] In a related aspect, compounds of Formula III, further where the compound is of Formula III, can be used in the preparation of a medicament for the treatment of a Jnk2-mediated disease or condition, such as atherosclerosis.

[0298] In a related aspect, compounds of Formula III, further where the compound is of Formula III, can be used in the preparation of a medicament for the treatment of a Jnk3-mediated disease or condition selected from the group consisting of inflammatory diseases including autoimmune diseases such as rheumatoid arthritis, inflammatory bowel syndrome, Crohn's disease, systemic lupus erythematosis, Sjogren's Syndrome, psoriasis and multiple sclerosis, airway inflammatory diseases such as asthma, allergy, pulmonary fibrosis, and chronic obstructive pulmonary disease, and inflammation in other organs, such as CNS inflammation, pancreatitis, nephritis, and hepatitis;
neurologic diseases such as stroke, cerebrovascular ischemia, and neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, and Huntington's disease; and neoplastic diseases such as prostate tumors and myeloid leukemia.

[0299] In a related aspect, compounds of Formula III, further where the compound is of Formula III, can be used in the preparation of a medicament for the treatment of a Kit-mediated disease or condition selected from the group consisting of malignancies, including mast cell tumors, small cell lung cancer, testicular cancer, gastrointestinal stromal tumors (GISTS), glioblastoma, astrocytoma, neuroblastoma, carcinomas of the female genital tract, sarcomas of neuroectodermal origin, colorectal carcinoma, carcinoma in situ, Schwann cell neoplasia associated with neurofibromatosis, acute myelocytic leukemia, acute lymphocytic leukemia, chronic myelogenous leukemia, mastocytosis, melanoma, and canine mast cell tumors, and inflammatory diseases, including asthma, rheumatoid arthritis, allergic rhinitis, multiple sclerosis, inflammatory bowel syndrome, transplant rejection, and hypereosinophilia.

[0300] The compounds of Formula I with kinase activity IC50 less than 10 M as determined in a standard assay described herein can be used to treat protein kinase mediated diseases and conditions related to the following protein kinases, for example without limitation:
Abl, related to chronic myeloid leukemia (CML), acute lymphoblastic leukemia (ALL) and acute myelogenous leukemia (AML);
Aktl, related to gastric, prostate, colorectal, ovarian, pancreatic and breast cancer, glioblastoma and leukemia, as well as schizophrenia and bipolar disorders, and also use in combination with other chemotherapeutic drugs;
Akt2, related to hyperglycemia due to peripheral insulin resistance and nonsuppressible hepatic glucose production accompanied by inadequate compensatory hyperinsulinemia, also related to pancreatic, ovarian and breast cancer;
Akt3, related to melanoma, prostate and breast cancer;
ALK, related to non-Hodgkin lymphomas such as diffuse large B-cell lymphoma and anaplastic large cell lymphoma;
Alk5, related to pancreatic and biliary cancers, and cutaneous T-cell lymphoma;
B-Raf. related to neurologic diseases such as ischemic stroke, multi-infarct dementia, head injury, spinal cord injury, Alzheimer's disease (AD), Parkinson's disease; neoplastic diseases including, but not limited to, melanoma, glioma, sarcoma, carcinoma (e.g.
lung, breast, pancreatic, renal), lymphoma (e.g. histiocytic lymphoma) and cancer of the thyroid, lung (e.g.
small cell lung cancer), liver, breast, ovary and colon, neurofibromatosis, myelodysplastic syndrome, leukemia, tumor angiogenesis; pain of neuropathic or inflammatory origin, including acute pain, chronic pain, and migraine; cardiovascular diseases including heart failure, cardiac hypertrophy, thrombosis (e.g. thrombotic microangiopathy syndromes), atherosclerosis, reperfusion injury; inflammation including, but not limited to, psoriasis, polycystic kidney disease (PKD), arthritis and autoimmune diseases and conditions, osteoarthritis, endometriosis, scarring, vascular restenosis, fibrotic disorders, rheumatoid arthritis, inflammatory bowel disease (IBD); immunodeficiency diseases, organ transplant rejection, graft versus host disease; renal or prostatic diseases including diabetic nephropathy, nephrosclerosis, glomerulonephritis, prostate hyperplasia; metabolic disorders, obesity;
infection, including, but not limited to Helicobacterpylori and Influenza virus, fever, sepsis;
pulmonary diseases including chronic obstructive pulmonary disease (COPD) and acute respiratory distress syndrome (ARDS); genetic developmental diseases such as Noonan's syndrome, Costello syndrome, (faciocutaneoskeletal syndrome), leopard syndrome, cardio-faciocutaneous syndrome (CFC), and neural crest syndrome abnormalities causing cardiovascular, skeletal, intestinal, skin, hair and endocrine diseases;
c-Raf-1, related to colorectal, ovarian, lung and renal cell carcinoma, acute myeloid leukemia, myelodysplastic syndromes, tumor angiogenesis, and neuroendocrine tumors such as medullary thyroid cancer, carcinoid, small cell lung cancer and pheochromocytoma;
Brk, related to breast and colon cancer, and head and neck squamous cell carcinoma;
Btk, related to X-linked agammaglobulinemia, acute lymphocytic leukemia, autoimmune diseases such as multiple sclerosis, systemic lupus erythematosis, rheumatoid arthritis, and Graves' disease, immune suppression in organ transplant, and drug sensitivity of B-lineage cells;
Cdk2, related to prostate, breast, colorectal and ovarian cancer;
Cdk4, related to glioblastoma (e.g. glioblastoma multiforme), anaplastic astrocytoma, and breast cancer;
Cdk5, related to Alzheimer's disease, amyotrophic lateral sclerosis and Lewy body disease;
Cdk6, related to glioblastoma multiforme, non-Hodgkin's lymphoma, splenic marginal zone lymphoma, T-cell lymphoblastic lymphoma (T-LBL) and T-cell acute lymphoblastic leukemia (T-ALL);
CHK1, related to DNA damage repair, sensitizes cells to chemotherapeutic agents;
Csk, related to colon and pancreatic carcinomas and autoimmune pathology such as type 1 diabetes, rheumatoid arthritis and systemic lupus erythematosus;
EGFR, related to breast, colorectal, bladder, prostate and non small cell lung cancer, squamous cell carcinomas of the head and neck cancer, oral cavity, and esophagus, and glioblastoma multiforme;

EphAl, related to head and neck squamous cell carcinoma, hepatoma and lung cancer;
EphA2, related to aberrant short-range contact-mediated axonal guidance, bladder, breast, prostate, colon, skin, cervical, ovarian, pancreatic and lung cancers, and metastatic melanoma;
EphB2, related to angiogenesis disorder (e.g. ocular angiogenesis disease such as retinopathy), and cancer (e.g. glioblastoma, breast and liver cancer);
EphB4, related to colorectal cancer (CRC), head and neck squamous cell carcinoma, and tumours of the prostate, breast, endometrium, and bladder;
Erk2, related to aberrant proliferation, differentiation, transcription regulation and development, and may be useful in treating inflammation, for example inflammation associated with Lyme neuroborreliosis, and in treating cancers, such as gastric cancer;
Fak, related to colon and breast tumors, and is also related to esophageal squamous cell carcinoma, melanoma, anaplastic astrocytoma, glioblastoma, ductal carcinoma in situ, prostate and hepatocellular carcinoma, and tumor metastases, and may also provide synergistic effects when used with other chemotherapeutic drugs;
FGFR1, related to 8pl 1 myeloproliferative syndrome;
FGFR2, related to Crouzon Syndrome, Jackson-Weiss Syndrome, Apert Syndrome, craniosynostosis, Pfeiffer Syndrome, acrocephalo syndactyly type V, and Beare-Stevenson Cutis Gyrata Syndrome;
FGFR3, related to angiogenesis, wound healing, achondroplasia, Muenke craniosynostosis, Crouzon syndrome, acanthosis nigricans, thanatophoric dysplasia, bladder carcinomas, and multiple myeloma;
FGFR4, related to cancer of the breast, lung, colon, medullary thyroid, pancreas, ovary, prostate, endometrium, and fallopian tube, head and neck squamous cell carcinomas and leiomyosarcoma;
Fltl, related to non-small cell lung carcinoma, prostate carcinoma, and colorectal cancer;
Flt3, related to acute myeloid leukemia, myelodysplastic syndrome, acute lymphoblastic leukemia;
Flt4, related to primary lymphoedema;
Fms, related to immune disorders, including rheumatoid arthritis, systemic lupus erythematosis (SLE), Wegener's granuloinatosis, and transplant rejection, inflammatory diseases including Chronic Obstructive Pulmonary Disease (COPD), emphysema, and atherosclerosis, metabolic disorders, including insulin resistance, hyperglycemia, and lipolysis, disorders of bone structure or mineralization, including osteoporosis, increased risk of fracture, hypercalcemia, and bone metastases, kidney diseases, including nephritis (e.g.
glomerulonephritis, interstitial nephritis, Lupus nephritis), tubular necrosis, diabetes-associated renal complications, and hypertrophy and cancers, including multiple myeloma, acute myeloid leukemia, chronic myeloid leukemia (CML), breast cancer, and ovarian cancer;

Frk, related to acute myeloid leukemia and type 1 diabetes;
Fyn, related to Alzheimer's disease, schizophrenia and prevention of metastases, e.g. in melanoma and squamous cell carcinoma;
GSK3 (Gsk3a and/or Gsk3(3), related to CNS disorders such as Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, diabetes type II, bipolar disorders, stroke, cancer, chronic inflammatory disease, leucopenia, schizophrenia, chronic pain, neuropathic pain, and traumatic head injury;
HCK, related to chronic myelogenous leukemia and acute lymphocytic leukemia;
Her2/Erbb2, related to prostate and breast cancer;
Her4/Erbb4, related to childhood medulloblastoma;
IGF1R, related to prostate cancer, hepatocellular carcinoma;
IKK beta, related to leukemia of T-cells, necrosis, insulin resistance, and malignant neoplasms;
Irak4, related to bacterial infections, immunodeficiency syndrome, Crohn's disease, ulcerative colitis, asthma, chronic bronchitis, cardio hypertrophy, and kidney hypertension;
Itk, related to allergic asthma;
Jakl, related to Hepatitis C virus infection;
Jak2, related to myeloproliferative disorders such as polycythaemia vera, myelofibrosis, essential thrombocythemia, myeloid metaplasia and leukemias, including acute lymphoblastic leukemia, chronic neutrophilic leukemia, juvenile myelomonocytic leukemia, CMML, Philadelphia chromosome-negative CML, megakaryocytic leukemia, and acute erythroid leukemia;
Jak3, related to X-linked severe combined immunodeficiency, myeloproliferative disorders, transplant rejection and autoimmune diseases such as rheumatoid arthritis, inflammatory bowel syndrome, Crohn's disease, systemic lupus erythematosis, ulcerative colitis, psoriasis and multiple sclerosis;
Jnk (Jnkl, Jnk2, Jnk3), related to metabolic diseases including type 1 diabetes, type 2 diabetes, metabolic syndrome, obesity, and hepatic steatosis; cardiovascular diseases such as atherosclerosis, ischemia (e.g. cerebrovascular ischemia, liver ischemia), reperfusion injury, cardiac hypertrophy; renal diseases such as chronic renal failure; neoplastic diseases and associated complications, including chemotherapy-induced hypoxia, prostate tumors, myeloid leukemia and cancers of the liver, bone, skin, brain, pancreas, lung breast, colon, prostate and ovary; transplant rejection; pain of neuropathic or inflammatory origin including acute and chronic pain; inflammatory and autoimmune diseases including age-related macular degeneration, rheumatoid arthritis, inflammatory bowel disease, ulcerative colitis, Crohn's disease, systemic lupus erythematosis, Sjogren's Syndrome, psoriasis, scleroderma, chronic thyroiditis, Grave's disease, myasthenia gravis, and multiple sclerosis, and inflammation in other organs including CNS inflammation, pancreatitis, nephritis, atopic dermatitis, and hepatitis; airway inflammatory diseases such as asthma, allergy, bronchitis, pulmonary fibrosis, chronic obstructive pulmonary disease; neurologic diseases such as stroke, cerebrovascular ischemia, neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis, dementia, senile chorea, head and spinal cord trauma, and Huntington's disease. More particularly, Jnk1 is related to type 1 diabetes, type 2 diabetes, metabolic syndrome, obesity and hepatic steatosis, Jnk2 is related to atherosclerosis, and Jnk3 is related to inflammatory diseases including autoimmune diseases such as rheumatoid arthritis, inflammatory bowel syndrome, Crohn's disease, systemic lupus erythematosis, Sjogren's Syndrome, psoriasis and multiple sclerosis, airway inflammatory diseases such as asthma, allergy, pulmonary fibrosis, and chronic obstructive pulmonary disease, and inflammation in other organs, such as CNS inflammation, pancreatitis, nephritis, and hepatitis; neurologic diseases such as stroke, cerebrovascular ischemia, and neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, and Huntington's disease; and neoplastic diseases such as prostate tumors and myeloid leukemia;
Kdr, related to anti-angiogenesis for treating solid tumor growth (e.g.
ovarian, lung, breast, prancreatic, prostate, colon, gastrointestinal stromal tumor, non small cell lung cancer, and epidermoid cancer), metastasis, psoriasis, rheumatoid arthritis, diabetic retinopathy and age related macular degeneration;
Kit, related to malignancies, including mast cell tumors, small cell lung cancer, testicular cancer, gastrointestinal stromal tumors (GISTs), glioblastoma, astrocytoma, neuroblastoma, carcinomas of the female genital tract, sarcomas of neuroectodermal origin, colorectal carcinoma, carcinoma in situ, Schwann cell neoplasia associated with neurofibromatosis, acute myelocytic leukemia, acute lymphocytic leukemia, chronic myelogenous leukemia, mastocytosis, melanoma, and canine mast cell tumors, and inflammatory diseases, including asthma, rheumatoid arthritis, allergic rhinitis, multiple sclerosis, inflammatory bowel syndrome, transplant rejection, and hypereosinophilia;
LCK, related to acute lymphoblastic leukemia, T-cell lymphoma, lymphopenia, renal carcinoma, colon carcinoma, severe combined immunodeficiency, multiple sclerosis, inflammatory bowel and type I diabetes;
MAP2K1, related to acute myeloid leukemia, breast, ovarian and liver cancer;
MAP2K2, related to cancer and inflammation;
MAP4K4, related to cancer and tumor metastasis, diabetes and metabolic syndrome;
MAPKAPK2, cancer (e.g. prostate, breast), stroke, menengitis, and inflammatory disorders;
Met, related to kidney, breast, bladder, non-small-cell lung, colorectal, and bladder cancers, and hepatocellular carcinoma;

Mnkl, related to conditions associated with heat shock, nutrient deprivation, oxidative or osmotic stress, and infection of mammalian cells (e.g. with viruses such as adenovirus (Ad) or influenza virus), and autoimmune diseases;
MLK1, related to neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease, and inflammatory disorders;
p38, related to acute coronary syndrome, stroke, atherosclerosis, and inflammatory autoimmune diseases such as rheumatoid arthritis, inflammatory bowel disease, and Crohn's disease;
PDGFR (PDGFRA, PDGFRB), related to idiopathic hypereosinophilic syndrome, chronic eosinophilic leukemia, glioma, gastrointestinal stromal tumors (GISTS), juvenile myelomonocytic leukemia, metastatic medulloblastoma, atherogenesis, and restenosis. More particularly, PDGFRA related to idiopathic hypereosinophilic syndrome, chronic eosinophilic leukemia, glioma, gastrointestinal stromal tumors (GISTs), juvenile myelomonocytic leukemia, metastatic medulloblastoma, atherogenesis, and restenosis, and PDGFRB related to idiopathic hypereosinophilic syndrome, chronic eosinophilic leukemia, juvenile myelomonocytic leukemia, and metastatic medulloblastoma;
PDPKl, related to cancer and diabetes;
Piml, related to cancers such as hematopoietic (e.g. acute myeloid and acute lymphoid leukemias) and prostate cancers, and non-Hodgkin's lymphomas;
Pim2, related to lymphomas;
Pim3, related to hepatocellular carcinoma;
PKC alpha, related to pituitary tumors and prefrontal cortical dysfunction such as distractibility, impaired judgment, impulsivity, and thought disorder, also may be used to sensitize chemotherapy in breast, colon, and non small cell lung cancers;
PKC beta, related to diabetic retinopathy;
PKC-theta, related to insulin resistance, T-cell lymphoma;
Plkl, related to cancers (e.g. lymphoma of the thyroid, non-Hodgkin's lymphomas, colorectal cancers, leukemias and melanoma), also useful as sensitizer in chemotherapy;
Pyk2, related to inflammation (e.g. osteoporosis, polycystic kidney disease, rheumatoid arthiritis and inflammatory bowel disease), CNS disease (e.g. Parkinson's disease and Alzheimer's disease), stroke and cancers (e.g. gliomas, breast cancer, and pancreatic cancer);
Ret, related to cancer of the thyroid, neuroblastoma, familial medullary thyroid carcinoma (FMTC), multiple endocrine neoplasia type IIA and IIB (MEN2A, MEN2B), and neurodegenerative disorders (e.g. Hirschsprung's disease, Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis);

ROCK (ROCK-1, ROCK-2), related to cancers (e.g. ovarian cancer, hepatocellular carcinoma, pancreatic cancer), ocular disease (e.g. glaucoma), cardiac hypertrophy, improved renal perfusion, transplant rejection, and acute respiratory distress syndrome;
Ron, related to cancer and inflammation;
Src, related to cancer and osteoporosis;
Stk6, related to gastric, bladder, breast, lung, CNS, ovarian, kidney, colon, prostate, pancreas, and cervical cancers, melanoma, leukemia, and neuroblastoma;
Syk, related to lymphomas (e.g. mantle cell lymphoma);
TEC, related to sepsis, septic shock, inflammation, rheumatoid arthritis, Crohn's disease, irritable bowel disease (IBD), and ulcerative colitis;
Tie2 (TEK), related to cancer, arthritis (e.g. rheumatoid arthritis), and atherosclerosis;
TrkA, related to pain (e.g. chronic pain, neuropathic pain), cancer, arthritis, diabetic retinopathy, macular degeneration and psoriasis;
Yes, related to various cancers including esophageal squamous cell carcinoma;
and Zap70, related to AIDS, systemic lupus erythematosus, myasthenia gravis, atherosclerosis, rejection of transplanted organs or tissues, allograft rejection including acute and chronic allograft rejection, graft versus host disease, rheumathoid arthritis, psoriasis, systemic sclerosis, atopic dermatitis, eczematous dermatitis, alopecia, and inflammation of the nasal mucus membrane, including all forms of rhinitis.

[0301] Additional aspects and embodiments will be apparent from the following Detailed Description and from the claims.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
As used herein the following definitions apply unless clearly indicated otherwise:

[0302] "Halogen" refer to all halogens, that is, chloro (Cl), fluoro (F), bromo (Br), or iodo (I).
[0303] "Hydroxyl" or "hydroxy" refer to the group -OH.

[0304] "Thiol" refers to the group -SH.

[0305] "Lower alkyl" alone or in combination means an alkane-derived radical containing from 1 to 6 carbon atoms (unless specifically defined) that includes a straight chain alkyl or branched alkyl.
The straight chain or branched alkyl group is attached at any available point to produce a stable compound. In many embodiments, a lower alkyl is a straight or branched alkyl group containing from 1-6, 1-4, or 1-2, carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl, t-butyl, and the like. A
"substituted lower alkyl" denotes lower alkyl that is independently substituted, unless indicated otherwise, with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents, attached at any available atom to produce a stable compound, wherein the substituents are selected from the group consisting of -F, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR , -SR , -OC(O)R , -OC(S)R
, -C(O)R , -C(S)R , -C(O)OR , -C(S)OR , -S(O)R , -S(0)2R , -C(O)NIIR , -C(S)NHR
, -C(O)NR R , -C(S)OR R , -S(0)2NHR , -S(0)2NR R , -C(NH)NHR , -C(NH)NRPR , -NHC(O)R , -NHC(S)R , -NR C(O)R , -NR C(S)R , -NHS(0)2R , NR S(O)2R , -NHC(O)NHR , -NHC(S)NHR , -NR C(O)NH2, -NR C(S)NH2, -NR C(O)NHR , -NR C(O)NHR , -NHC(O)NR R , -NHC(O)NR
R , -NR C(O)NR R , -NR C(S)NR R , -NHS(O)2NHR , -NR S(O)2NH2, -NR. S(O)2NHR , -NHS(0)2NR R , -NR S(0)2NR R , NHR , NR R , -Re, -Rf, and -R9. Furthermore, possible substitutions include subsets of these substitutions, such as are indicated herein, for example, in the description of compounds of Formula III, attached at any available atom to produce a stable compound. For example "fluoro substituted lower alkyl" denotes a lower alkyl group substituted with one or more fluoro atoms, such as perfluoroalkyl, where preferably the lower alkyl is substituted with 1, 2, 3, 4 or 5 fluoro atoms, also 1, 2, or 3 fluoro atoms. While it is understood that substitutions are attached at any available atom to produce a stable compound, when optionally substituted alkyl is an R group of a moiety such as -OR (e.g. alkoxy), -SR (e.g. thioalkyl), -NHR
(e.g. alkylamino), -C(O)NHR, and the like, substitution of the alkyl R group is such that substitution of the alkyl carbon bound to any 0, S, or N of the moiety (except where N is a heteroaryl ring atom) excludes substituents that would result in any 0, S, or N of the substituent (except where N is a heteroaryl ring atom) being bound to the alkyl carbon bound to any 0, S, or N of the moiety.
"C2_6 alkyl" denotes lower alkyl containing 2-6 carbon atoms. A "substituted C2_6 alkyl" denotes optionally substituted lower alkyl containing 2-6 carbon atoms. A "substituted methyl" denotes methyl that is independently substituted, unless indicated otherwise, with 1, 2, or 3 substituents, wherein the substituents are selected as per optionally substituted lower alkyl.

[0306] "C1_3 alkylene" refers to a divalent alkane-derived radical containing 1-3 carbon atoms, straight chain or branched, from which two hydrogen atoms are taken from the same carbon atom or from different carbon atoms. C1.3 alkylene includes methylene -CH2-, ethylene -CH2CH2-, propylene -CH2CH2CH2-, and isopropylene -CH(CH3)CH2- or -CH2CH(CH3)-. C1_3 alkylene substituted with one or more substituents indicates C1_3 alkylene that is independently substituted, with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents as indicated, attached at any available atom to produce a stable compound.

[0307] "Lower alkenyl" alone or in combination means a straight or branched hydrocarbon containing 2-6 carbon atoms (unless specifically defined) and at least one, preferably 1-3, more preferably 1-2, most preferably one, carbon to carbon double bond. Carbon to carbon double bonds may be either contained within a straight chain or branched portion. Examples of lower alkenyl groups include ethenyl, propenyl, isopropenyl, butenyl, and the like. A
"substituted lower alkenyl"
denotes lower alkenyl that is independently substituted, unless indicated otherwise, with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents, attached at any available atom to produce a stable compound, wherein the substituents are selected from the group consisting of -F, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2a -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NHNNH2, -OR , -SR , -OC(O)R , -OC(S)R , -C(O)R , -C(S)R , -C(O)OR , -C(S)OR , -S(O)R , -S(0)2R , -C(O)NHR , -C(S)NHR , -C(O)NR R , -C(S)OR R , -S(0)2NHR , -S(0)2NR R , -C(NH)NHR , -C(NH)NRPR , NHC(O)R , -NHC(S)R , -NR C(O)R , -NR
C(S)R , -NHS(0)2R , -NR S(0)2R , -NHC(O)NHR , -NHC(S)NHR , NR C(O)NH2, -NR C(S)NH2, NR C(O)NHR , -NR C(S)NHR , -NHC(O)NR R , -NHC(O)NR R , -NR C(O)NR R , -NR C(S)NR R , -NHS(O)2NHR , -NR S(O)2NH2, NR S(O)2NHR , -NHS(O)2NR R , -NR S(0)2NR R , -NHR , -NR R , -Rd, -R, and -R9. Further, possible substitutions include subsets of these substitutions, such as are indicated herein, for example, in the description of compounds of Formula III, attached at any available atom to produce a stable compound. For example "fluoro substituted lower alkenyl" denotes a lower alkenyl group substituted with one or more fluoro atoms, where preferably the lower alkenyl is substituted with 1, 2, 3, 4 or 5 fluoro atoms, also 1, 2, or 3 fluoro atoms. While it is understood that substitutions are attached at any available atom to produce a stable compound, substitution of alkenyl groups are such that -F, -C(O)-, -C(S)-, -C(NEE)-, -S(O)-, -S(O)2-, -0-, -S-, or N (except where N is a heteroaryl ring atom), are not bound to an alkene carbon thereof. Further, where alkenyl is a substituent of another moiety or an R
group of a moiety such as -OR, -NHR, -C(O)R, and the like, substitution of the moiety is such that any -C(O)-, -C(S)-, -S(O)-, -S(O)2-, -0-, -S-, or N thereof (except where N is a heteroaryl ring atom) are not bound to an alkene carbon of the alkenyl substituent or R group. Further, where alkenyl is a substituent of another moiety or an R group of a moiety such as -OR, -NHR, -C(O)NHR, and the like, substitution of the alkenyl R
group is such that substitution of the alkenyl carbon bound to any 0, S, or N
of the moiety (except where N is a heteroaryl ring atom) excludes substituents that would result in any 0, S, or N of the substituent (except where N is a heteroaryl ring atom) being bound to the alkenyl carbon bound to any 0, S, or N of the moiety. An "alkenyl carbon" refers to any carbon within an alkenyl group, whether saturated or part of the carbon to carbon double bond. An "alkene carbon"
refers to a carbon within an alkenyl group that is part of a carbon to carbon double bond.

[0308] "Lower alkynyl" alone or in combination means a straight or branched hydrocarbon containing 2-6 carbon atoms (unless specifically defined) containing at least one, preferably one, carbon to carbon triple bond. Examples of alkynyl groups include ethynyl, propynyl, butynyl, and the like. A "substituted lower alkynyl" denotes lower alkynyl that is independently substituted, unless indicated otherwise, with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents, attached at any available atom to produce a stable compound, wherein the substituents are selected from the group consisting of -F, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR , -SR , -OC(O)R
, -OC(S)R , -C(O)R , -C(S)R , -C(O)OR , -C(S)OR , -S(O)R , -S(O)2R , -C(O)NHR , -C(S)NHR , -C(O)NR R , -C(S)NR R , -S(0)2NHR , -S(O)2NR R , -C(NH)NHR , -C(NH)NRRR , -NHC(O)R , -NHC(S)R , -NR C(O)R , -NR C(S)R , -NHS(0)2R , -NR S(0)2R , -NHC(O)NHR , -NHC(S)NHR , -NR C(O)NH2i -NR C(S)NH2, -NR C(O)NHR , -NR C(S)NHR , NHC(O)NR R , -NHC(S)NR R
, -NR C(O)NR R , -NR C(S)NR R , -NHS(0)2NHR , -NR S(O)2NH2, NR S(O)2NHR , -NHS(0)2NR R , -NR S(O)2NR R , -NHR , -NR R , -Rd, -Re, and -R9. Further, possible substitutions include subsets of these substitutions, such as are indicated herein, for example, in the description of compounds of Formula III, attached at any available atom to produce a stable compound. For example "fluoro substituted lower alkynyl" denotes a lower alkynyl group substituted with one or more fluoro atoms, where preferably the lower alkynyl is substituted with 1, 2, 3, 4 or 5 fluoro atoms, also 1, 2, or 3 fluoro atoms. While it is understood that substitutions are attached at any available atom to produce a stable compound, substitution of alkynyl groups are such that -F, -C(O)-, -C(S)-, -C(NH)-, -S(O)-, -S(O)2-, -0-, -S-, or N (except where N is a heteroaryl ring atom) are not bound to an alkyne carbon thereof. Further, where alkynyl is a substituent of another moiety or an R group of a moiety such as -OR, -NUR, -C(O)R, and the like, substitution of the moiety is such that any -C(O)-, -C(S)-,-S(O)-, -S(O)2-, -0-, -S-, or N thereof (except where N is a heteroaryl ring atom) are not bound to an alkyne carbon of the alkynyl substituent or R group. Further, where alkynyl is a substituent of another moiety or an R group of a moiety such as -OR, -NHR, -C(O)NHR, and the like, substitution of the alkynyl R group is such that substitution of the alkynyl carbon bound to any 0, S, or N of the moiety (except where N is a heteroaryl ring atom) excludes substituents that would result in any 0, S, or N of the substituent (except where N is a heteroaryl ring atom) being bound to the alkynyl carbon bound to any 0, S, or N of the moiety. An "alkynyl carbon" refers to any carbon within an alkynyl group, whether saturated or part of the carbon to carbon triple bond. An "alkyne carbon" refers to a carbon within an alkynyl group that is part of a carbon to carbon triple bond.

[0309] "Cycloalkyl" refers to saturated or unsaturated, non-aromatic monocyclic, bicyclic or tricyclic carbon ring systems of 3-10, also 3-8, more preferably 3-6, ring members per ring, such as cyclopropyl, cyclopentyl, cyclohexyl, adamantyl, and the like. A "substituted cycloalkyl" is a cycloalkyl that is independently substituted, unless indicated otherwise, with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents, attached at any available atom to produce a stable compound, wherein the substituents are selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2i -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2i -C(NH)NH2, -OR , -SR , -OC(O)R , -OC(S)R , -C(O)R , -C(S)R , -C(O)OR , -C(S)OR , -S(O)R , -S(0)2R , -C(O)NHR , -C(S)NHR , -C(O)NR R , -C(S)NR R , -S(0)2NHR , -S(0)2NR R , -C(NH)NRR , -C(NH)NRPR , -NHC(O)R , -NHC(S)R , -NR C(O)R , -NR
C(S)R , NHS(O)2R , NR S(O)2R , -NHC(O)NHR , -NHC(S)N R , -NR C(O)NH2, -NR C(S)NH2, -NR C(O)NHR , -NR C(S)NHR , -NHC(O)NR R , -NHC(S)NR R , -NR C(O)NR R , -NR C(S)NR R , -NHS(0)2NHR , NR S(O)2NH2, -NR S(0)2NHR , -NHS(0)2NR R , -NR S(0)2NR R , -NHR , -NR R , -Rd, -Re, -R", and -R9.

[0310] "Heterocycloalkyl" refers to a saturated or unsaturated non-aromatic cycloalkyl group having from 5 to 10 atoms in which from 1 to 3 carbon atoms in the ring are replaced by heteroatoms of 0, S
or N, and are optionally fused with benzo or heteroaryl of 5-6 ring members.
Heterocycloalkyl is also intended to include oxidized S or N, such as sulfinyl, sulfonyl and N-oxide of a tertiary ring nitrogen.
Heterocycloalkyl is also intended to include compounds in which a ring carbon may be oxo substituted, i.e. the ring carbon is a carbonyl group, such as lactones and lactams. The point of attachment of the heterocycloalkyl ring is at a carbon or nitrogen atom such that a stable ring is retained. Examples of heterocycloalkyl groups include, but are not limited to, morpholino, tetrahydrofuranyl, dihydropyridinyl, piperidinyl, pyrrolidinyl, pyrrolidonyl, piperazinyl, dihydrobenzofuryl, and dihydroindolyl. A "substituted heterocycloalkyl" is a heterocycloalkyl that is independently substituted, unless indicated otherwise, with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents, attached at any available atom to produce a stable compound, wherein the substituents are selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR , -SR , -OC(O)R , -OC(S)R , -C(O)R , -C(S)R , -C(O)OR , -C(S)OR
, -S(O)R , -S(0)2R , -C(O)NHR , -C(S)NHR , -C(O)NR R , -C(S)NR R , -S(O)2NHR , -S(O)2NR R
, -C(NH)NHR , -C(NH)NRPR , -NHC(O)R , -NHC(S)R , -NR C(O)R , -NR C(S)R , -NHS(0)2R , -NR S(0)2R , NHC(O)NHR , -NHC(S)NHR , -NR C(O)NH2, -NR C(S)NH2, -NR C(O)NHR , -NR C(S)NHR , -NHC(O)NR R , -NHC(S)NR R , -NR C(O)NR R , -NR C(S)NR R , -NHS(0)2NHR , -NR S(0)2NH2i -NR S(0)2NHR , -NHS(0)2NR R , -NR S(0)2NR R , -NHR
, -NR R , -Rd, -Re, -R, and -Rg.

[0311] "Aryl" alone or in combination refers to a monocyclic or bicyclic ring system containing aromatic hydrocarbons such as phenyl or naphthyl, which may be optionally fused with a cycloalkyl of preferably 5-7, more preferably 5-6, ring members. "Arylene" is a divalent aryl. A "substituted aryl" is an aryl that is independently substituted, unless indicated otherwise, with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents, attached at any available atom to produce a stable compound, wherein the substituents are selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2i -C(S)NH2, -S(O)2NH2i -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR , -SR , -OC(O)R , -OC(S)R , -C(O)R , -C(S)R , -C(O)OR , -C(S)OR , -S(O)R , -S(0)2R , -C(O)NHR , -C(S)NHR , -C(O)NR R , -C(S)NR R , -S(0)2NHR , -S(0)2NR R , -C(NH)NHR , -C(NH)NHR , -NHC(O)R , -NHC(S)R , NR C(O)R , -NR
C(S)R , -NHS(0)2R , NR S(O)2R , -NHC(O)NIR2 , NHC(S)NHR NR C(O)NH2, -NR C(S)NH2, -NR C(O)NHR , -NR C(S)NHR , NHC(O)NR R , -NHC(S)NR R , -NR C(O)NR R , -NR C(S)NR R , -NHS(0)2NHR , -NR S(O)2NH2i -NR S(0)2NHR , -NHS(0)2NR R , -NR S(0)2NR R , -NHR , -NR R , -Rd, -Re, -R', and -R5. A "substituted arylene"
is a divalent substituted aryl.

[0312] "Heteroaryl" alone or in combination refers to a monocyclic aromatic ring structure containing 5 or 6 ring atoms, or a bicyclic aromatic group having 8 to 10 atoms, containing one or more, preferably 1-4, more preferably 1-3, even more preferably 1-2, heteroatoms independently selected from the group consisting of 0, S, and N. Heteroaryl is also intended to include oxidized S
or N, such as sulfinyl, sulfonyl and N-oxide of a tertiary ring nitrogen. A
carbon or nitrogen atom is the point of attachment of the heteroaryl ring structure such that a stable compound is produced.
Examples of heteroaryl groups include, but are not limited to, pyridinyl, pyridazinyl, pyrazinyl, quinaoxalyl, indolizinyl, benzo[b]thienyl, quinazolinyl, purinyl, indolyl, quinolinyl, pyrimidinyl, pyrrolyl, pyrazolyl, oxazolyl, thiazolyl, thienyl, isoxazolyl, oxathiadiazolyl, isothiazolyl, tetrazolyl, imidazolyl, triazolyl, furanyl, benzofuryl, and indolyl. "Nitrogen containing heteroaryl" refers to heteroaryl wherein any heteroatoms are N. "Heteroarylene" is a divalent heteroaryl. A "substituted heteroaryl" is a heteroaryl that is independently substituted, unless indicated otherwise, with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents, attached at any available atom to produce a stable compound, wherein the substituents are selected from the group consisting of halogen, -OH, -NH2, NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR , -SR , -OC(O)R , -OC(S)R , -C(O)R , -C(S)R , -C(O)OR , -C(S)OR , -S(O)R , -S(0)2R , -C(O)NHR , -C(S)NHR , -C(O)NR R , -C(S)NR R , -S(0)2NHR , -S(0)2NR R , -C(NH)NHR , -C(NH)NRR , -NHC(O)R , NHC(S)R , NR C(O)R
, -NR C(S)R , -NHS(0)2R , -NR S(0)2R , -NHC(O)NHR , -NHC(S)NHR , -NR C(O)NH2, -NR C(S)NH2, -NR C(O)NHR , -NR C(S)NHR , -NHC(O)NR R , -NHC(O)NR R , -NR
C(O)NR R , -NR C(S)NR R , -NHS(0)2NHR , -NR S(O)2NH2, -NR S(0)2NHR , -NHS(0)2NR R , NR S(0)2NR R , -NHR , -NR R , -Rd, -Re, -R1, and -Rn. "Substituted heteroarylene" is a divalent substituted heteroaryl.

[0313] The variables R , RP, R , Rd, R, Rf and R5 as used in the description of optional substituents for alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl are defined as follows:

each R , BY, and Re are independently selected from the group consisting of Rd, R, R', and Rg, or RP
and Re combine with the nitrogen to which they are attached to form a 5-7 membered heterocycloalkyl or a 5 or 7 membered nitrogen containing heteroaryl, wherein the 5-7 membered heterocycloalkyl or 5 or 7 membered nitrogen containing heteroaryl are optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of halogen, -NO2, -CN, -OH, -NH2, -OR , -SR , -NHR , -NR R , -R", and -R'';

each Rd is independently lower alkyl, wherein lower alkyl is optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2 or 3 substituents selected from the group consisting of fluoro, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -ORk, -SRk, -OC(O)Rk, -OC(S)Rk, -C(O)Rk, -C(S)R k, -C(O)ORk, -C(S)ORk, -S(O)R', -S(O)2Rk, -C(O)NHRk, -C(S)NHR', -C(O)NRkRk, -C(S)NRkRk, -S(O)2NHRk, -S(O)2NRkRk, -C(NH)NHRk, -C(NH)NRmR", -NHC(O)Rk, NHC(S)Rk, -NRkC(O)Rk -NRkC(S)Rk, -NHS(O)2Rk, -NRkS(O)2Rk, -NHC(O)NHRk, -NHC(S)NHRk, -NRkC(O)NH2, -NRkC(S)NH2, -NRkC(O)NHRk, -NRkC(S)NHRk, NHC(O)NRkRk, NHC(S)NRkRk, NRkC(O)NRkRk, NRkC(S)NRkRk, -NHS(O)2NH Rk, -NRkS(0)2NH2, -NR kS(O)2NHRk, -NHS(O)2NRkRk, -NRkS(O)2NRkRk, -NHRk, -NRkRk, -R', and -R';

each Re is independently lower alkenyl, wherein lower alkenyl is optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2 or 3 substituents selected from the group consisting of fluoro, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR', -SR k' -OC(O)Rk, -OC(S)R', -C(O)Rk, -C(S)e, -C(O)OR', -C(S)ORk, -S(O)e, -S(0)2e, -C(O)NHRk, -C(S)NHRk, -C(O)NRkRk, -C(S)NRkRk, -S(O)2NHRk, -S(O)2NRkRk, -C(NH)NHRk, -C(NH)NRmR , NHC(O)R', NHC(S)Rk, -NRkC(O)Rk, -NRkC(S)Rk, -NHS(O)2Rk, -NRkS(O)2Rk, -NHC(O)NHRk, -NHC(S)NHRI, -NR'`C(O)NH2, -NRkC(S)NH2, NRkC(O)NHRk, -NRkC(S)NHRk, NHC(O)NRkRk, -NHC(S)NRkRk, -NRkC(O)NR'`Rk, -NR kC(S)NRkRk, -NHS(O)2NHRk, -NRkS(0)2NH2i -NRkS(O)2NHRk, NHS(O)2NRkRk, -NRkS(O)2NRkRk, -NHRk, -NRkRk, -Rh, and -R';

each Reis independently lower alkynyl, wherein lower alkynyl is optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2 or 3 substituents selected from the group consisting of fluoro, -OH, -NH2, NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -ORk, -SR k' -OC(O)Rk, -OC(S)Rk, -C(O)Rk, -C(S)Rk, -C(O)ORk, -C(S)ORk, -S(O)Rk, -S(O)2Rk, -C(O)NHR', -C(S)NHRk, -C(O)NRkRk, -C(S)NRkRk, -S(O)2NHRk, -S(O)2NRkRk, -C(NH)NHRk, -C(NH)NRmRn, -NHC(O)Rk, -NHC(S)Rk, NRkC(O)Rk, -NRkC(S)Rk, NHS(O)2Rk, -NRkS(O)2Rk, NHC(O)NHRk, -NHC(S)NHRk, -NRkC(O)NH2, -NR kC(S)NH2i -NR kC(O)NHRk, -NR.kC(S)NHRk, -NHC(O)NR'`Rk, NHC(S)NRkRk, NRkC(O)NRkRk, -NK C(S)NR R", -NHS(O)2NHR", -NR"S(O)2NH2, -NR"S(O)2NHR", -NHS(O)2NR"R", -NRkS(O)2NRkRk -NHRk, -NRkRk, -Rh, and -R';

each Rg is independently selected from the group consisting of cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2 or 3 substituents selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2i -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2i -ORk, -SRk, -OC(O)Rk, -OC(S)Rk, -C(O)Rk, -C(S)Rk, -C(O)ORk, -C(S)ORk, -S(O)Rk, -S(O)2Rk, -C(O)NHRk, -C(S)NHRk, -C(O)NRkRk, -C(S)NRkRk, -S(O)2NHRk, -S(O)2NRkRk, -C(NH)NHRk, -C(NH)NR"'R , -NHC(O)Rk, -NHC(S)Rk, -NRkC(O)Rk, NRkC(S)Rk, -NHS(O)2Rk, -NRkS(O)2Rk, -NHC(O)NHRk, -NHC(S)NHR', -NRkC(O)NH2, -NR kC(S)NH2, -NRkC(O)NHRk, -NRkC(S)NHRk, -NHC(O)NRkRk, -NHC(S)NRkRk, -NRkC(O)NRkRk, -NRkC(S)NRkRk, -NHS(O)2NHRk, -NRkS(O)2NH2, NRkS(O)2NHRk, -NHS(O)2NRkRk, -NRkS(0)2NRkRk, -NHRk, -Nee, -Rh, -R', and -R

wherein Rk, R"', and R" at each occurrence are independently selected from the group consisting of Rh, R', and R', or R' and R" combine with the nitrogen to which they are attached form a 5-7 membered heterocycloalkyl or a 5 or 7 membered nitrogen containing heteroaryl, wherein the 5-7 membered heterocycloalkyl or 5 or 7 membered nitrogen containing heteroaryl are optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of halogen, -NO2, -CN, -OH, -NH2, OR , -SR", -NHR", -NR"R", -R', and -R'';
wherein each Rh is independently lower alkyl optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of fluoro, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2a -C(S)NH2, -S(O)2NH2i -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OW, -SR', -OC(O)Rr, -OC(S)R', -C(O)R', -C(S)Rt, -C(O)ORr, -C(S)ORr, -S(O)W, -S(O)2Rr, -C(O)NHRT, -C(S)NHRT, -C(O)NRrRF, -C(S)NRrRr, -S(O)2NHRr, -S(O)2NRrRr, -C(NH)NHRT, -C(NH)NRSRt, -NHC(O)Rr, -NHC(S)Rr, -NRrC(O)Rr, -NRrC(S)Rr, -NHS(O)2Rr, -NRrS(0)2Rr, -NHC(O)NHR', -NHC(S)NHRr, -NR`C(O)NH2i -NRrC(S)NH2, -NRrC(O)NHRr, -NR`C(S)NHRr, -NHC(O)NR'W, -NHC(S)NRrRr, -NRFC(O)NR`W, -NR`C(S)NRrRr, -NHS(O)2NHRr, -NRrS(O)2NH2, -NRrS(O)2NHRr, -NHS(O)2NRrRr, -NRrS(O)2NRrRr, -NW, -NRrRr, -R', and -Ri;

wherein each R' is independently selected from the group consisting of lower alkenyl and lower alkynyl, wherein lower alkenyl or lower alkynyl are optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2 or 3 substituents selected from the group consisting of fluoro, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2i -NHS(O)2NH2, -C(NH)NH2, -OW, -SW, -OC(O)R', -OC(S)R', -C(O)W, -C(S)Rr, -C(O)ORr, -C(S)ORr, -S(O)RT, -S(O)2Rr, -C(O)NHRr, -C(S)NHRr, -C(O)NR'Rr, -C(S)NR'R', -S(O)2NHRr, -S(O)2NRrRr, -C(NH)NHRr, -C(NH)NRSRt, NHC(O)Rr, -NHC(S)Rr, -NRrC(O)Rr, -NRrC(S)Rr, -NHS(0)2W, -NR'S(O)2Rr, -NHC(O)NHRr, -NHC(S)NHRr, NRrC(O)NH2, NRrC(S)NH2, -NRrC(O)NHRr, -NRTC(S)NHRr, -NHC(O)NRTr, -NHC(S)NR'Rt, -NRrC(O)NRrRr, -NRrC(S)NRTRr, -NHS(O)2NHRr, -NR`S(O)2NH2, -NRrS(0)2NHRT, -NHS(O)2NRIRr, -NRrS(0)2NRrRr, -NHRr, -NRrRr, and -R';

wherein each R is independently selected from the group consisting of cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2 or 3 substituents selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2i -C(NH)NH2, -OR', -SR', -OC(O)Rr, -OC(S)Rr, -C(O)W, -C(S)R', -C(O)ORr, -C(S)ORr, -S(O)W, -S(O)2Rr, -C(O)NHRr, -C(S)NHRr, -C(O)NRrRr, -C(S)NRrRr, -S(O)2NHRr, -S(O)2NRrRr, -C(NH)NHRr, -C(NH)NRSRt, -NHC(O)R', -NHC(S)R', NRrC(O)Rr, -NRrC(S)Rr, -NHS(O)2Rr, -NRrS(O)2Rr, -NHC(O)NHR', -NHC(S)NHRr, -NRTC(O)NH2i -NR'C(S)NH2, -NRrC(O)NHRr, -NRTC(S)NHR', -NHC(O)NRrRr, -NHC(S)NRrRr, -NR'C(O)NR'Rt, -NRrC(S)NRrlr, -NHS(O)2NHRr, -NR'S(O)2NH2, -NRrS(O)2NHRr, -NHS(O)2NRTRr, NRrS(O)2NRrRr, -NHRr, -NRrRr, cycloalkylamino, and -R';

wherein each Rr, RS, and Rt at each occurrence are independently selected from the group consisting of lower alkyl, C3_6 alkenyl, C3_6 alkynyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein lower alkyl is optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of RY, fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the lower alkyl carbon bound to any 0, S, or N, of -OR', -SW, -C(O)ORr, -C(S)OR', -C(O)NHRr, -C(S)NHRT, -C(O)NRrRr, -C(S)NRrRr, -S(O)2NHRr, -S(O)2NRrRr, -C(NH)NHRr, -NRrC(O)Rr, -NRTC(S)R', -NRrS(O)2Rr, -NHC(O)NHRt, -NHC(S)NHR', -NRrC(O)NH2, -NRrC(S)NH2, -NR'C(O)NHR, NRrC(S)NHRr, -NHC(O)NR'Rr, -NHC(S)NRrRr, -NRrC(O)NRRr, -NRrC(S)NRrRr, -NHS(0)2NHRr, -NRrS(O)2NH2, -NRrS(O)2NHRr, -NHS(0)2NR'Rr, -NR'S(O)2NRrRr, -NHRr, or NR`Rr is selected from the group consisting of fluoro and -RY, and wherein C3_6 alkenyl or C3.6 alkynyl are optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of -RY, fluoro, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the C3_6 alkenyl or C3-6 alkynyl carbon bound to any 0, S, or N, of -OW, -SW, C(O)ORt, -C(S)ORr, -C(O)NHRT, -C(S)NHIZr, -C(O)NRrRr, -C(S)NRrRr, -S(O)2NHRr, -S(O)2NR'Rr, -C(NH)NHR', -NRrC(O)Rr, -NR'C(S)R', -NRrS(O)2Rr, -NHC(O)NHRr, -NHC(S)NHR', -NRrC(O)NH2, NRrC(S)NH2, NWC(O)NIMr, -NRrC(S)NW, -NHC(O)NR'Rr, NHC(S)NR`R', -NRrC(O)NRrRr, -NR`C(S)NRTR', -NHS(O)2NHRr, -NRrS(O)2NH2, -NRrS(0)2NHRr, -NHS(O)2NRTRr, -NRrS(O)2NR'Rr, -NHRr, or -NRRT is selected from the group consisting of fluoro, lower alkyl, fluoro substituted lower alkyl, and -RY, and wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, or RS and Rt combine with the nitrogen to which they are attached form a 5-7 membered heterocycloalkyl or a 5 or 7 membered nitrogen containing heteroaryl, wherein the 5-7 membered heterocycloalkyl or 5 or 7 membered nitrogen containing heteroaryl are optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of halogen, -NO2, -CN, -OH, -NH2, OR , -SR , -NHR , -NR R , -RX, and -RY;

wherein each R is independently selected from the group consisting of lower alkyl, C3-6 alkenyl, C3-6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein lower alkyl is optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of RY, fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the lower alkyl carbon bound to the 0 of -OR , S of -SR , or N of -NHR is fluoro or -RI, and wherein C3-6 alkenyl or C3-6 alkynyl are optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of -RY, fluoro, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the C3-6 alkenyl or C3-6 alkynyl carbon bound to the 0 of -OR , S of -SR , or N of -NHR is fluoro, lower alkyl, fluoro substituted lower alkyl, or -RY, and wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;

wherein each R" is selected from the group consisting of lower alkyl, lower alkenyl and lower alkynyl, wherein lower alkyl is optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of -RI, fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, and wherein lower alkenyl or lower alkynyl are optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of -RY, fluoro, -OH, -NH2, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
wherein each RY is selected from the group consisting of cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl are optionally substituted with one or more, preferably 1, 2, 3, 4 or 5, also 1, 2, or 3 substituents selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, lower alkyl, fluoro substituted lower alkyl, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino.

[03141 In some embodiments, all occurrences of optionally substituted lower alkyl, optionally substituted C2_6 alkyl, optionally substituted lower alkenyl, or optionally substituted lower alkynyl are optionally substituted with one or more, also 1, 2 or 3 groups or substituents selected from the group consisting of fluoro, -NO2, -CN, -OR la, -SR' NR'aRla, -OC(O)Rla, -OC(S)R'a, -C(O)Rla, -C(S)R la, -C(O)OR a, -C(S)OR a, -C(O)NR'aR'a, -C(S)NR'aR'a, _S(O)2NR'aR'a, _C(NH)NRtaR]a, -NR'aC(O)Rla, -NR'aC,(S)Rla, -NR'aS(O)2R1a, -NRIaC(O)NRlaRla, -NR'aC(S)NRIaRla, -NR'aS(O)2NRlaRla, -S(O)Rla, -S(O)2R'a, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl and heteroaryl are optionally substituted with one or more, also 1, 2 or 3 groups or substituents selected from the group consisting of halogen, NO2, -CN, -OR',,, -SR h, -NR'aR'a, -OC(O)R'a, -OC(S)R'a, -C(O)R'a, -C(S)R'a, -C(O)OR'a, -C(S)OR'a, _C(O)NR'aR'a, -C(S)NR'aR'a, _S(O)2NR'aR1a, -C(NH)NR1aR1a, NRlaC(O)Rla, -NR'aC(S)Rla, -NR1aS(O)2RIa, -NRIaC(O)NRIaRta, _NR1aC(S)NR1aR'a, _NR'aS(O)2NR1aRta, -S(O)R'a, -S(O)2R'a, -Rlb, and lower alkyl optionally substituted with one or more, also 1, 2 or 3 groups or substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and -R'b, and all occurrences of optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted 5-7 membered heterocycloalkyl, optionally substituted aryl, optionally substituted arylene, optionally substituted heteroaryl, optionally substituted heteroarylene, or optionally substituted 5 or 7 membered nitrogen containing heteroaryl are optionally substituted with one or more, also 1, 2, or 3 groups or substituents selected from the group consisting of halogen, -NO2, -CN, -OR'a, -SR'a, -NR'aRla, -OC(O)Rla, -OC(S)Rla, -C(O)Rla, -C(S)RIa, _C(O)ORIa, _C(S)ORIa, _C(O)NRIaRIa, _C(S)NRIaRIa, -S(O)2NRIaRla, _C(NH)NRIaRIa, NRIaC(O)Rla, _NRIaC(S)Rla, -NRIaS(O)2RIa, _NRIaC(O)NRIaRIa, _NRIaC(S)NRIaRla, _ R1aS(O)2NRIaRIa, _S(O)Rla, -S(O)2RIa, -Rlb, and lower alkyl optionally substituted with one or more, also 1, 2 or 3 groups or substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and -R 1b, wherein Rla is selected from the group consisting of hydrogen, provided, however, that hydrogen is not bound to any of C(S), C(O), S(O), or S(O)2 of -OC(O)RIa, -OC(S)RIa, -C(O)Rla, -C(S)Rla, -NRIaC(O)Rla, -NRIaC(S)RIa, -NRIaS(O)2RIa, -S(O)R", or -S(O)2RIa, -Rlb, and lower alkyl optionally substituted with one or more, also 1, 2 or 3 groups or substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and -Rlb, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -OR", -SRIa, -NRIaRIa, -C(O)ORIa, -C(S)ORIa, -C(O)NRIaRIa, _C(S)NRIaRIa, -S(O)2NRIaRIa, _C(Iq3)NRlaRIa, NRIaC(O)Rla, NRIaC(S)Rla, _ IaS(O)2RIa, -NRIaC(O)NRIaRIa, -NRIaC(S)NRIaRIa, or NRhS(O)2NRIaRh, is fluoro or -R1b, and wherein -RIb is selected from the group consisting of cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl and heteroaryl are optionally substituted with one or more, also 1, 2 or 3 groups or substituents selected from the group consisting of halogen, -CN, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino.

[03151 In some embodiments, all occurrences of optionally substituted lower alkyl, optionally substituted C2_6 alkyl, optionally substituted lower alkenyl, or optionally substituted lower alkynyl are optionally substituted with one or more, also 1, 2 or 3 groups or substituents selected from the group consisting of fluoro, -CN, -OR", -SRIa, -NRIaRIa, -C(O)Rla, -C(S)Rla, -C(O)ORIa, -C(O)NRIaRIa, -C(S)NR1aRla, _S(O)2NRIaRIa, -NRIaC(O)Rla, -NRIaC(S)Rla. -NRIaS(O)2RIa, -S(O)Ra, -S(O)2RIa, cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl and heteroaryl are optionally substituted with one or more, also 1, 2 or 3 groups or substituents selected from the group consisting of halogen, -CN, -ORIa, -SR a, -NRIaRIa, -C(O)Rla, -C(S)R a, -C(O)OR a, _C(O)NRIaRIa, -C(S)NRIaRla, _S(O)2NRIaRIa, -NRIaC(O)Rla, -NRIaC(S)Rla, _NRlaS(0)2RIa, _S(O)Rla, -S(O)2RIa, -Rlb, and lower alkyl optionally substituted with one or more, also 1, 2 or 3 groups or substituents selected from the group consisting of fluoro, -OH, NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and _RIb, and all occurrences of optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted 5-7 membered heterocycloalkyl, optionally substituted aryl, optionally substituted arylene, optionally substituted heteroaryl, optionally substituted heteroarylene, or optionally substituted 5 or 7 membered nitrogen containing heteroaryl are optionally substituted with one or more, also 1, 2, or 3 groups or substituents selected from the group consisting of halogen, -CN, -OR1a, -SRIa, -NR'aRla, -C(O)Rla, -C(S)Rla, -C(O)ORIa, -C(O)NRIaRia, -C(S)NRiaRla, -S(O)2NRIaRIa, -NR1aC(O)Rla, -NRIaC(S)Rla, -NR1aS(O)2R1a, -S(O)Rla, -S(O)2RIa, -R1', and lower alkyl optionally substituted with one or more, also 1, 2 or 3 groups or substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and -R 1b, wherein R1a is selected from the group consisting of hydrogen, provided, however, that hydrogen is not bound to any of C(S), C(O), S(O), or S(O)2 of -C(O)Ra, -C(S)Rla, NRIaC(O)Rla, NR1aC(S)RIa, -NR1aS(O)2RIa, -S(O)Rla, or -S(O)2R'a, -RIb, and lower alkyl optionally substituted with one or more, also 1, 2 or 3 groups or substituents selected from the group consisting of fluoro, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and -Rlb, provided, however, that any substitution of the alkyl carbon bound to 0, S, or N of -ORIa, -SRIa, _NR1aRla, -C(O)OR"', -C(O)NRIaR1a, -C(S)NRIaRIa, -S(O)2NRIaRIa, -NR1aC(O)Rla, -NRIaC(S)RIa, or -NRIaS(O)2RIa, is fluoro or -R'b, and wherein -RIb is selected from the group consisting of cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl and heteroaryl are optionally substituted with one or more, also 1, 2 or 3 groups or substituents selected from the group consisting of halogen, -CN, -OH, -NH2, lower alkoxy, fluoro substituted lower alkoxy, lower alkylthio, fluoro substituted lower alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino.

[03161 "Lower alkoxy" denotes the group -OR', where RZ is lower alkyl.
"Substituted lower alkoxy" denotes lower alkoxy in which RZ is lower alkyl substituted with one or more substituents as indicated herein, for example, in the description of compounds of Formula III, including descriptions of substituted cycloalkyl, cycloheteroalkyl, aryl and heteroaryl, attached at any available atom to produce a stable compound. Preferably, substitution of lower alkoxy is with 1, 2, 3, 4, or 5 substituents, also 1, 2, or 3 substituents. For example "fluoro substituted lower alkoxy" denotes lower alkoxy in which the lower alkyl is substituted with one or more fluoro atoms, where preferably the lower alkoxy is substituted with 1, 2, 3, 4 or 5 fluoro atoms, also 1, 2, or 3 fluoro atoms. While it is understood that substitutions on alkoxy are attached at any available atom to produce a stable compound, substitution of alkoxy is such that 0, S, or N (except where N is a heteroaryl ring atom), are not bound to the alkyl carbon bound to the alkoxy O. Further, where alkoxy is described as a substituent of another moiety, the alkoxy oxygen is not bound to a carbon atom that is bound to an 0, S, or N of the other moiety (except where N is a heteroaryl ring atom), or to an alkene or alkyne carbon of the other moiety.

[03171 "Lower alkylthio" denotes the group -SR", where Raa is lower alkyl.
"Substituted lower alkylthio" denotes lower alkylthio in which Raa is lower alkyl substituted with one or more substituents as indicated herein, for example, in the description of compounds of Formula Ill, including descriptions of substituted cycloalkyl, cycloheteroalkyl, aryl and heteroaryl, attached at any available atom to produce a stable compound. Preferably, substitution of lower alkylthio is with 1, 2, 3, 4, or 5 substituents, also 1, 2, or 3 substituents. For example "fluoro substituted lower alkylthio"
denotes lower alkylthio in which the lower alkyl is substituted with one or more fluoro atoms, where preferably the lower alkylthio is substituted with 1, 2, 3, 4 or 5 fluoro atoms, also 1, 2, or 3 fluoro atoms. While it is understood that substitutions on alkylthio are attached at any available atom to produce a stable compound, substitution of alkylthio is such that 0, S, or N
(except where N is a heteroaryl ring atom), are not bound to the alkyl carbon bound to the alkylthio S. Further, where alkylthio is described as a substituent of another moiety, the alkylthio sulfur is not bound to a carbon atom that is bound to an 0, S, or N of the other moiety (except where N is a heteroaryl ring atom), or to an alkene or alkyne carbon of the other moiety.

[0318] "Amino" or "amine" denotes the group -NH2. "Mono-alkylamino" denotes the group -NHRbb where Rbb is lower alkyl. "Di-alkylamino" denotes the group -NRbbR00, where Rbb and Re are independently lower alkyl. "Cycloalkylamino" denotes the group -NRddRee, where Rd' and Ree combine with the nitrogen to form a 5-7 membered heterocycloalkyl, where the heterocycloalkyl may contain an additional heteroatom within the ring, such as 0, N, or S, and may also be further substituted with lower alkyl. Examples of 5-7 membered heterocycloalkyl include, but are not limited to, piperidine, piperazine, 4-methylpiperazine, morpholine, and thiomorpholine. While it is understood that when mono-alkylamino, di-alkylamino, or cycloalkylamino are substituents on other moieties that are attached at any available atom to produce a stable compound, the nitrogen of mono-alkylamino, di-alkylamino, or cycloalkylamino as substituents is not bound to a carbon atom that is bound to an 0, S, or N of the other moiety.

[0319] A "nitrogen protecting group" is a chemical group covalently bound to a nitrogen atom of a compound that is used to protect the nitrogen from reaction during a synthetic step. The nitrogen protecting group may be added to a compound and removed in a subsequent step by methods known to those of skill in the art. Nitrogen protecting groups include, without limitation, carbamates, amides, N-sulfonyl derivatives, groups of formula -C(O)OR, wherein R is, for example, methyl, ethyl, t-butyl, benzyl, phenylethyl, CH2=CHCH2-, and the like, groups of the formula -C(O)R', wherein R' is, for example, methyl, phenyl, trifluoromethyl, and the like, groups of the formula -SO2R", wherein R" is, for example, tolyl, phenyl, trifluoromethyl, 2,2,5,7,8-pentamethylchroman-6-yl, 2,3,6-trimethyl-4-methoxyphenyl, and the like, and silanyl containing groups, such as 2-trimethylsilylethoxymethyl, t-butyldimethylsilyl, triisopropylsilyl, and the like. Other suitable nitrogen protecting groups may be found in texts such as T. W. Greene & P. G. M. Wuts, Protective Groups in Organic Synthesis, John Wiley & Sons, 1991.

[0320] As used herein, the term "composition" refers to a formulation suitable for administration to an intended animal subject for therapeutic purposes that contains at least one pharmaceutically active compound and at least one pharmaceutically acceptable carrier or excipient.

[0321] The term "pharmaceutically acceptable" indicates that the indicated material does not have properties that would cause a reasonably prudent medical practitioner to avoid administration of the material to a patient, taking into consideration the disease or conditions to be treated and the respective route of administration. For example, it is commonly required that such a material be essentially sterile, e.g., for injectibles.

[0322] In the present context, the term "therapeutically effective" or "effective amount" indicates that the materials or amount of material is effective to prevent, alleviate, or ameliorate one or more symptoms of a disease or medical condition, and/or to prolong the survival of the subject being treated.

[0323] In the present context, the terms "synergistically effective" or "synergistic effect" indicate that two or more compounds that are therapeutically effective, when used in combination, provide improved therapeutic effects greater than the additive effect that would be expected based on the effect of each compound used by itself.

[0324] As used herein, the terms "ligand" and "modulator" are used equivalently to refer to a compound that changes (i.e., increases or decreases) the activity of a target biomolecule, e.g., an enzyme such as a kinase. Generally a ligand or modulator will be a small molecule, where "small molecule refers to a compound with a molecular weight of 1500 daltons or less, or preferably 1000 daltons or less, 800 daltons or less, or 600 daltons or less. Thus, an "improved ligand" is one that possesses better pharmacological and/or pharmacokinetic properties than a reference compound, where "better" can be defined by one skilled in the relevant art for a particular biological system or therapeutic use.

[0325] In the context of binding compounds and ligands, the term "derivative"
or "derivative compound" refers to a compound having a chemical structure that contains a common core chemical structure as a parent or reference compound, but differs by having at least one structural difference, e.g., by having one or more substituents added and/or removed and/or replaced, and/or by having one or more atoms replaced with different atoms. Unless clearly indicated to the contrary, the term "derivative" does not mean that the derivative is synthesized using the parent compound as a starting material or as an intermediate, although in some cases, the derivative may be synthesized from the parent.

[0326] Thus, the term "parent compound" refers to a reference compound having structural features also found in the derivative compound. Often but not always, a parent compound has a simpler chemical structure than the derivative.

[0327] By "chemical structure" or "chemical substructure" is meant any definable atom or group of atoms that constitute an individually identifiable portion of a molecule, such as a substituent moiety, a core which is optionally substituted, and the like. Normally, chemical substructures of a ligand can have a role in binding of the ligand to a target molecule, or can influence the three-dimensional shape, electrostatic charge, and/or conformational properties of the ligand.

[0328] The term "binds" in connection with the interaction between a target and a potential binding compound indicates that the potential binding compound associates with the target to a statistically significant degree as compared to association with proteins generally (i. e., non-specific binding).
Thus, the term "binding compound" refers to a compound that has a statistically significant association with a target molecule. Preferably a binding compound interacts with a specified target with a dissociation constant (KD) of 1 mM or less, 1 M or less, 100 nM or less, 10 nM or less, or 1 nM or less.

[0329] In the context of compounds binding to a target, the terms "greater affinity" and "selective"
indicates that the compound binds more tightly than a reference compound, or than the same compound in a reference condition, i.e., with a lower dissociation constant.
In some embodiments, the greater affinity is at least 2, 3, 4, 5, 8, 10, 50, 100, 200, 400, 500, 1000, or 10,000-fold greater affinity.

[0330] As used herein in connection with compounds of the invention, the term "synthesizing" and like terms means chemical synthesis from one or more precursor materials.
Further, by "assaying" is meant the creation of experimental conditions and the gathering of data regarding a particular result of the experimental conditions. For example, enzymes can be assayed based on their ability to act upon a detectable substrate. A compound or ligand can be assayed based on its ability to bind to a particular target molecule or molecules.

[0331] As used herein, the term "modulating" or "modulate" refers to an effect of altering a biological activity, especially a biological activity associated with a particular biomolecule such as a protein kinase. For example, an agonist or antagonist of a particular biomolecule modulates the activity of that biomolecule, e.g., an enzyme, by either increasing (e.g.
agonist, activator), or decreasing (e.g. antagonist, inhibitor) the activity of the biomolecule, such as an enzyme. Such activity is typically indicated in terms of an inhibitory concentration (IC50) or excitation concentration (JC50) of the compound for an inhibitor or activator, respectively, with respect to, for example, an enzyme.

[0332] In the context of the use, testing, or screening of compounds that are or may be modulators, the term "contacting" means that the compound(s) are caused to be in sufficient proximity to a particular molecule, complex, cell, tissue, organism, or other specified material that potential binding interactions and/or chemical reaction between the compound and other specified material can occur.
[0333] As used herein in connection with amino acid or nucleic acid sequence, the term "isolate"
indicates that the sequence is separated from at least a portion of the amino acid and/or nucleic acid sequences with which it would normally be associated.

[0334] In connection with amino acid or nucleic sequences, the term "purified"
indicates that the subject molecule constitutes a significantly greater proportion of the biomolecules in a composition than the proportion observed in a prior composition, e.g., in a cell culture.
The greater proportion can be 2-fold, 5-fold, 10-fold, or more than 10-fold, with respect to the proportion found in the prior composition.

1. General [0335] The present invention concerns compounds of Formula I and all sub-generic formulae, compounds of Formula TI and all sub-generic formulae, and compounds of Formula III and all sub-generic formulae that are modulators of protein kinases, for example without limitation, the compounds are modulators of at least one of the kinases selected from the group consisting of Abl, Aktl, Akt2, Akt3, ALK, Alk5, B-Raf, Brk, Btk, Cdk2, CDK4, CDK5, CDK6, CHKI, c-Raf-1, Csk, EGFR, EphAl, EphA2, EphB2, EphB4, Erk2, Fak, FGFRI, FGFR2, FGFR3, FGFR4, Fltl, F1t3, Flt4, Fins, Frk, Fyn, Gsk3a, Gsk3[3, HCK, Her2/Erbb2, Her4/Erbb4, IGF1R, IKK beta, Irak4, Itk, Jakl, Jak2, Jak3, Jnkl, Jnk2, Jnk3, Kdr, Kit, LCK, MAP2K1, MAP2K2, MAP4K4, MAPKAPK2, Met, Mnkl, MLK1, p38, PDGFRA, PDGFRB, PDPK1, Piml, Pim2, Pim3, PKC alpha, PKC beta, PKC theta, Plkl, Pyk2, Ret, ROCKI, ROCK2, Ron, Src, Stk6, Syk, TEC, Tie2, TrkA, Yes, and Zap70, and the use of such compounds in the treatment of diseases or conditions.

II. Kinase targets and indications of the invention [0336] Protein kinases play key roles in propagating biochemical signals in diverse biological pathways. More than 500 kinases have been described, and specific kinases have been implicated in a wide range of diseases or conditions (i.e., indications), including for example without limitation, cancer, cardiovascular disease, inflammatory disease, neurological disease, and other diseases. As such, kinases represent important control points for small molecule therapeutic intervention.
Description of specific target protein kinases contemplated by the present invention follow:

[0337] Abl: Target Abl (i.e., Abelson Murine Leukemia Viral Oncogene Homolog 1) is a 122.9 kDa tyrosine kinase encoded by chromosome 9g34.1 (symbol: ABL1.) The mature protein comprises SH3 (i.e., Src homology region 3) and SH2 (i.e., Src homology region 2) domains and the TK (i.e., tyrosine kinase) domain.

[0338] OMIM indicates Abl is expressed ubiquitously and can be localized to the nucleus where it binds DNA. Accordingly, Abl has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Alterations of the gene ABL1 by a t(9;22)(q34;ql 1) chromosomal rearrangement or viral transduction lead to malignant transformation, as in chronic myeloid leukemia (CML). The kinase activity of Abl is negatively regulated by the constituent SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation occurs in greater than 90% of chronic myelogeneous leukemia, 25 to 30% of adult and 2 to 10% of childhood acute lymphoblastic leukemia (ALL), and rare cases of acute myelogenous leukemia.
The translocation results in the head-to-tail fusion of the BCR and ABL genes (Chissoe et al., Genomics 1995, 27: 67-82). The DNA-binding activity of Abl is regulated by CDC2-mediated phosphorylation suggesting a cell cycle function for ABL. Welch & Wang (Cell 1993, 75: 779-790) showed that the tyrosine kinase activity of nuclear Abl is regulated in the cell cycle through a specific interaction with the retinoblastoma protein RB 1. A domain in the C-terminus of RB 1 binds to the ATP-binding lobe of, Abl, resulting in kinase inhibition. The RB-ABL interaction is not affected by the viral oncoproteins that bind to RB. Hyperphosphorylation of RB correlates with release of Abl and activation of Abl in S phase cells. In the nucleus Abl can enhance transcription, and this activity is inhibited by RB.
Thus, nuclear Abl is an S phase-activated tyrosine kinase that might participate directly in the regulation of transcription. (Online Mendelian Inheritance in Man, OMIM (TM).
Johns Hopkins University, Baltimore, MD. MIM Number: 189980: 12/20/2005:. World Wide Web URL:
http://www3.ncbi.nlm.nih.gov/omim/). Abl inhibitors may be useful in treating leukemia, including chronic myelogeneous leukemia, acute lymphoblastic leukemia, and acute myelogenous leukemia.
[0339] Aktl: Target kinase Akt1 (i.e., v-akt murine thymoma viral oncogene homolog 1) is a 55.7 kDa STK encoded by chromosome 14q32.32 (symbol: AKT1). Aktl is also known as protein kinase B-alpha, or PKB-alpha. OMIM indicates phosphoinositide 3-kinases (i.e., PI3Ks) generate specific inositol lipids implicated in the regulation of cell growth, proliferation, survival, differentiation, and cytoskeletal changes. One of the best characterized targets of P13K lipid products is the protein kinase AKT, or protein kinase B (PKB). In quiescent cells, PKB resides in the cytosol in a low-activity conformation. Upon cellular stimulation, PKB is activated through recruitment to cellular membranes by P13K lipid products and by phosphorylation by 3-prime phosphoinositide-dependent kinase-1. Most proliferating cells are programmed to undergo apoptosis unless specific survival signals are provided. PDGF promotes cellular proliferation, inhibits apoptosis, and activates the RAS/PIK3/AKT1/IKBKA/NFKB1 pathway (Romashkova and Makarov, Nature 1999, 401:
86-90).
In this pathway, NFKB 1 does not induce c-myc and apoptosis, but instead induces putative antiapoptotic genes. In response to PDGF, Aktl transiently associates with IKBK and induces IKBK
activation (OMIM MIM Number: 164730: 10/26/2005)Aberrant Aktl activity is correlated with) including gastric and prostate tumors, colorectal, ovarian, pancreatic, and breast cancer, glioblastoma and leukemia. Sun et al., Am J Pathol 2001, 159(2):431-7, report that significantly increased AKT1 kinase activity was detected in primary carcinomas of prostate (16 of 30), breast (19 of 50), and ovary (11 of 28). Tanno et al., Cancer Res 2001, 61(2):589-93, provide evidence for a link between AKT
signaling and the regulation of IGF-IR expression and demonstrate that active AKT promotes the invasiveness of pancreatic cancer cells through the up-regulation of IGF-IR
expression. Neri et al., Mol Cancer Res 2003, 1(3):234-46, suggest that an up-regulation of the PI3K/AKTl pathway might be one of the survival mechanisms responsible for the onset of resistance to chemotherapeutic and differentiating therapy in patients with acute leukemia. Aktl activity is also important in schizophrenia and bipolar disorders. Emamian et al., Nat Genet 2004, 36(2):115-6, present convergent evidence for a decrease in AKT1 protein levels and levels of phosphorylation of GSK3beta at Ser9 in the peripheral lymphocytes and brains of individuals with schizophrenia; a significant association between schizophrenia and an AKT1 haplotype associated with lower AKT1 protein levels; and a greater sensitivity to the sensorimotor gating-disruptive effect of amphetamine, conferred by AKT1 deficiency. Aktl inhibitors may be useful in treating cancer, including gastric, prostate, colorectal, ovarian, pancreatic and breast cancer, glioblastoma and leukemia, and also for use in combination with other chemotherapeutic drugs.

[03401 Akt2: Target kinase Akt2 (i.e., v-akt murine thymoma viral oncogene homolog 2) is a 55.8 kDa STK encoded by chromosome 19g13.1-13.2 (symbol: AKT2). Akt2 is also known as protein kinase B beta, PKB-beta. OMIM indicates that the Akt2 isoform of Akt (see e.g., Aktl and Akt3) is enriched in insulin-responsive tissues and has been implicated in the metabolic actions of insulin.
Glucose homeostasis depends on insulin responsiveness in target tissues, most importantly, muscle and liver. The critical initial steps in insulin action include phosphorylation of scaffolding proteins and activation of phosphatidylinositol 3-kinase. These early events lead to activation of the serine-threonine protein kinase Akt, also known as protein kinase B. Cho et al., Science 2001, 292:1728-1731, showed that mice deficient in Akt2 are impaired in the ability of insulin to lower blood glucose because of defects in the action of the hormone on liver and skeletal muscle.
Ablation of Akt2 in mice results in a mild but statistically significant fasting hyperglycemia due to peripheral insulin resistance and nonsuppressible hepatic glucose production accompanied by inadequate compensatory hyperinsulinemia (OMIM MIM Number: 164731: 10/26/2005). Arboleda et al., Cancer Res 2003, 63(1):196-206 showed that AKT2 transfected breast and ovarian cancer cells demonstrated increased adhesion and invasion through collagen IV because of up-regulation of betal integrins and that AKT2 cells were more metastatic than control cells in vivo. Yamamoto et al., Clin Cancer Res 2004, 10(8):2846-50 studied the prognostic significance of Akt2 and activated Akt expression in pancreatic ductal adenocarcinoma (PDAC), concluding that p-Akt expression is a significant prognostic indicator for PDAC and inhibition of Akt is a possible molecular approach for treatment of PDAC. Yuan et al., Oncogene 2000, 19(19):2324-30, demonstrate that activation of AKT2 is a common occurrence in human ovarian cancer and that the PI 3-kinase/Akt pathway may be an important target for ovarian cancer intervention. Yuan et al., J Biol Chem 2003, 278(26):23432-40, demonstrate that constitutively active AKT2 renders cisplatin-sensitive A2780S ovarian cancer cells resistant to cisplatin, whereas phosphatidylinositol 3-kinase inhibitor or dominant negative AKT21 sensitizes A2780S and cisplatin-resistant A2780CP cells to cisplatin-induced apoptosis through regulation of the ASK1/JNK/p38 pathway. Akt2 inhibitors may be useful in treating cancer, including ovarian and pancreatic cancers, pancreatic ductal adenocarcinoma, and metastases of breast and ovarian cancer, and also for use in combination with other chemotherapeutic drugs, where such use sensitizes the tumor cells to the effects of the other chemotherapeutics.

[03411 Akt3: Target kinase Akt3 (i.e., v-akt murine thymoma viral oncogene homolog 3) is a 55.8 kDa STK encoded by chromosome 1g43-q44 (symbol:AKT3); Akt3 is also known as PKB gamma.
Akt3 was identified as a protein kinase with high homology with the protein kinases A and C, hence, the name PKB. Akt3 comprises a PH domain that preferentially binds Ptdlns(3,4,5)P3 and Ptdlns(3,4)P2 over other phosphatidyl inositols (PIs). In quiescent cells, PKB
resides in the cytosol in a low-activity conformation.

[03421 Upon cellular stimulation, PKB is activated through recruitment to cellular membranes by P13K lipid products and phosphorylation by 3'-phosphoinositide-dependent kinase-1 (PDK1). Active PKB then appears to detach from the plasma membrane and to translocate through the cytosol to the nucleus.

[03431 Stahl et al. have found that selective activation of the Akt3 protein promotes cell survival and tumor development in 43 to 60% of nonfamilial melanomas. The predominant Akt isoform active in melanomas was identified by showing that small interfering RNA (siRNA) against only Akt3, and not Aktl or Akt2, lowered the amount of phosphorylated (active) Akt in melanoma cells. The amount of active Akt3 increased progressively during melanoma tumor progression with highest levels present in advanced-stage metastatic melanomas. Mechanisms of Akt3 deregulation occurred through a combination of overexpression of Akt3 accompanying copy number increases of the gene and decreased PTEN protein function occurring through loss or haploinsufficiency of the PTEN gene.
Targeted reduction of Akt3 activity with siRNA or by expressing active PTEN
protein stimulated apoptotic signaling, which reduced cell survival by increasing apoptosis rates thereby inhibiting melanoma tumor development. Therefore, Akt3 is a selective target in melanoma cells which provides therapeutic opportunities for subjects in advanced stages of the disease (Stahl et al., Cancer Res. 2004, 64:7002-10). Nakatani et al., J Biol Chem 1999, 274(31):21528-32 showed that in estrogen receptor-deficient breast cancer cells and androgen-insensitive prostate cells, the amount of Akt3 enzymatic activity was approximately 20-60-fold higher than in cells that were estrogen- or androgen-responsive. These and other results indicate that Akt3 may contribute to the more aggressive clinical phenotype of the estrogen receptor-negative breast cancers and androgen-insensitive prostate carcinomas and inhibitors may provide therapeutic benefits in treating these cancers. Akt3 inhibitors may be useful in treating cancer, including estrogen receptor-negative breast cancers, androgen-insensitive prostate carcinomas, and melanomas.

[03441 ALK: Target kinase ALK (i.e., anaplastic lymphoma kinase) is a 176.4 kDa receptor tyrosine kinase encoded by chromosome 2p23 (symbol: ALK). ALK appears to play a role in the development of the central nervous system. Perkins, et al., show that systemic ALCL is highly associated with anaplastic lymphoma kinase (ALK) gene translocations with over-expression of ALK
protein. Anaplastic large cell lymphoma (ALCL) comprises 10-15% of childhood non-Hodgkin lymphomas (NHL) (Perkins et al., Br J Haematol 2005, 131(5):624-7). Marzec et al., states aberrant expression of the ALK tyrosine kinase as a chimeric protein with nucleophosmin (NPM) and other partners plays a key role in malignant cell transformation of T-lymphocytes and other cells. Further, studies with inhibitors of NPM/ALK enzyme activity suggest ALK as an attractive therapeutic target in T-cell lymphomas and other malignancies that express the kinase in an active form (Marzec et al., Lab Invest 2005, 85(12):1544-54). ALK inhibitors may be useful in treating cancer, including anaplastic large cell lymphoma and other T cell lymphomas.

[03451 AIk5: Target kinase A1k5 (i.e., Activin receptor-like kinase 5) is a 56.0 kDa STK encoded by chromosome 9q22 (symbol: TGFBRI). Alk5, the gene for which was isolated by Franzen et al (Cell 1993, 75: 681-692), is also known as transforming growth factor-beta receptor, type I, from which term the gene symbol derives. Among other activities, Alk5 mediates the induction of multiple genes involved in cell-matrix interactions. Alk5 inhibitors may be useful in treating pancreatic and biliary cancers and cutaneous T-cell lymphoma.

[03461 B-Raf: Target kinase B-Raf (i.e., v-raf murine sarcoma viral oncogene homolog B 1) is a 84.4 kDa serine/threonine kinase encoded by chromosome 7q34 (symbol: BRAF).
The mature protein comprises RBD (i.e., Ras binding domain), C1 (i.e., protein kinase C
conserved region 1) and STK (i.e., serine/threonine kinase) domains.

[0347] Target kinase B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus and may play a role in the postsynaptic responses of hippocampal neurons.
As such, genes of the RAF family encode kinases that are regulated by Ras and mediate cellular responses to growth signals. Indeed, B-Raf kinase is a key component of the RAS->Raf->MEK->ERK/MAP kinase signaling pathway, which plays a fundamental role in the regulation of cell growth, division and proliferation, and, when constitutively activated, causes tumorigenesis. Among several isoforms of Raf kinase, the B-type, or B-Raf, is the strongest activator of the downstream MAP kinase signaling.

[0348] The BRAF gene is frequently mutated in a variety of human tumors, especially in malignant melanoma and colon carcinoma. The most common reported mutation was a missense thymine (T) to adenine (A) transversion at nucleotide 1796 (T1796A; amino acid change in the B-Raf protein is Val<600> to Glu<600>) observed in 80% of malignant melanoma tumors. Functional analysis reveals that this transversion is the only detected mutation that causes constitutive activation of B-Raf kinase activity, independent of RAS activation, by converting B-Raf into a dominant transforming protein.

[0349] Niihori et al., report that in 43 individuals with cardio-facio-cutaneous (CFC) syndrome, they identified two heterozygous KRAS mutations in three individuals and eight BRAF
mutations in 16 individuals, suggesting that dysregulation of the RAS-RAF-ERK pathway is a common molecular basis for the three related disorders (Niihori et al., Nat Genet. 2006, 38(3):294-6).

[0350] B-Raf inhibitors may be useful in treating neurologic diseases such as ischemic stroke, multi-infarct dementia, head injury, spinal cord injury, Alzheimer's disease (AD), Parkinson's disease;
neoplastic diseases including, but not limited to, melanoma, glioma, sarcoma, carcinoma (e.g. lung, breast, pancreatic, renal), lymphoma (e.g. histiocytic lymphoma) and cancer of the thyroid, lung (e.g.
small cell lung cancer), liver, breast, ovary and colon, neurofibromatosis, myelodysplastic syndrome, leukemia, tumor angiogenesis; pain of neuropathic or inflammatory origin, including acute pain, chronic pain, and migraine; cardiovascular diseases including heart failure, cardiac hypertrophy, thrombosis (e.g. thrombotic microangiopathy syndromes), atherosclerosis, reperfusion injury;
inflammation including, but not limited to, psoriasis, polycystic kidney disease (PKD), arthritis and autoimmune diseases and conditions, osteoarthritis, endometriosis, scarring, vascular restenosis, fibrotic disorders, rheumatoid arthritis, inflammatory bowel disease (IBD);
immunodeficiency diseases, organ transplant rejection, graft versus host disease; renal or prostatic diseases including diabetic nephropathy, nephrosclerosis, glomerulonephritis, prostate hyperplasia; metabolic disorders, obesity; infection, including, but not limited to Helicobacterpylori and Influenza virus, fever, sepsis;
pulmonary diseases including chronic obstructive pulmonary disease (COPD) and acute respiratory distress syndrome (ARDS); genetic developmental diseases such as Noonan's syndrome, Costello syndrome, (faciocutaneoskeletal syndrome), leopard syndrome, cardio-faciocutaneous syndrome (CFC), and neural crest syndrome abnormalities causing cardiovascular, skeletal, intestinal, skin, hair and endocrine diseases.

[03511 c-Raf-1: Target kinase c-Raf-1 (i.e., v-raf marine sarcoma viral oncogene homolog 1) is a 73.0 kDa STK encoded by chromosome 3p25 (symbol: RAF1). c-Raf-1 can be targeted to to the mitochondria by BCL2 (i.e., oncogene B-cell leukemia 2) which is a regulator of apoptotic cell death.
Active c-Raf-1 improves BCL2-mediated resistance to apoptosis, and c-Raf-1 phosphorylates BAD
(i.e., BCL2-binding protein). c-Raf-1 is implicated in carcinomas, including colorectal, ovarian, lung and renal cell carcinoma. C-Raf-1 is also implicated as an important mediator of tumor angiogenesis (Hood, J.D. et al., 2002, Science 296, 2404). C-Raf-1 inhibitors may also be useful for the treatment of acute myeloid leukemia and myelodysplastic syndromes (Crump, Carr Pharm Des 2002, 8(25):2243-8). Raf-1 activators may be useful as treatment for neuroendocrine tumors, such as medullary thyroid cancer, carcinoid, small cell lung cancer and pheochromocytoma (Kunnimalaiyaan et al., Anticancer Drugs 2006, 17(2):139-42). C-Raf-1 inhibitors may be useful in treating colorectal, ovarian, lung and renal cell carcinoma, acute myeloid leukemia, myelodysplastic syndromes, tumor angiogenesis, and neuroendocrine tumors such as medullary thyroid cancer, carcinoid, small cell lung cancer and pheochromocytoma.

[03521 Brk: Target kinase Brk (i.e, breast tumor kinase, also known as PTK6) is a 51.8 kDa non-receptor tyrosine kinase encoded by human chromosome 20g13.3 (symbol: BRK).
The kinase has an SH3 domain, an SH2 domain, and a catalytic domain. In normal tissues, the expression of Brk (breast tumor kinase) is restricted to differentiating epithelial cells of the skin and gastrointestinal tract.
According to Harvey and Crompton, Brk is expressed in over 60% of breast carcinoma tissue samples and breast tumour cell lines, but not normal mammary tissue or benign lesions.
They used RNA
interference to efficiently and specifically downregulate Brk protein levels in breast carcinoma cells, and determined that this results in a significant suppression of their proliferation (Harvey and Crompton, Oncogene, 2003, 22(32): 5006-5010). Lin et al. identified protein-tyrosine kinases that may be involved in the development and progression of head and neck squamous cell carcinoma (HNSCC), and their findings suggest that the signaling pathways mediated through EphAl, Brk, and Ron may be involved in the development and progression of HNSCC (Lin et al., Arch Otolaryngol Head Neck Surg. 2004, 130(3):311-6). Llor et al. examined BRK expression in the normal gastrointestinal tract, colon tumor cell lines, and primary colon tumor samples and showed BRK is expressed in normal epithelial cells of the gastrointestinal tract that are undergoing terminal differentiation. BRK expression also increased during differentiation of the Caco-2 colon adenocarcinoma cell line. Modest increases in BRK expression were detected in primary colon tumors by RNase protection, in situ hybridization, and immunohistochemical assays (Llor et al., Clin Cancer Res. 1999, 5(7):1767-77). Brk inhibitors may be useful in treating cancer, such as breast and colon cancer, and head and neck squamous cell carcinoma.

[0353] Btk: Target kinase Btk (i.e., Bruton's tyrosine kinase) is a 76.3 kDa tyrosine kinase encoded by chromosome Xg21.33-q22 (symbol: BTK). The mature kinase comprises a PH
(i.e., Pleckstrin homology) domain, a BTK (i.e., Bruton's tyrosine kinase motif) motif, two SH3 domains, and a TK
domain. Mao et al. determined the X-ray crystal structure of the Btk kinase domain in its unphosphorylated state to 2.1-angstrom resolution (Mao et al., J. Biol. Chem., 2001, 276:41435).
[0354] As a member of the BTK/Tec family of protein tyrosine kinases (i.e., PTKs), cytoplasmic Btk is involved in signal transduction pathways regulating growth and differentiation of B-lineage lymphoid cells (Rawlings, D. J., and Witte, O. N., 1994. Immunol. Rev. 138:105-119; Kurosaki, T., 1997, Curr Opin. Immunol 9:309-318; Uckun, F. M., 1998, Biochemical Pharmacology 56:683-691).
As such, Btk participates in signal transduction pathways initiated by the binding of a variety of extracellular ligands to their cell surface receptors. For example, following ligation of B cell antigen receptors (BCR), Btk activation by the concerted actions of the PTKs Lyn and Syk (Kurosaki, T.
(1997) Curr Opin. Immunol. 9, 309-318) is required for induction of phospholipase C-[gamma]2 mediated calcium mobilization (Kurosaki, T., 1997, Curr Opin. Immunol. 9:309-318). Furthermore, Btk regulates B cell antigen receptor-mediated JNK1 response through Racl and phospholipase C-gamma2 activation.

[0355] Mutations in the human BTK gene are the cause of X-linked agammaglobulinemia (XLA), a male immune deficiency disorder characterized by a lack of mature, immunoglobulin producing, peripheral B cells (Tsukada, S., et al. (1993) Cell 72, 279-290; and Vetrie, D., et al. (1993) Nature 361, 226-233) and associated with a failure of Ig heavy chain rearrangement.
Patients are unusually prone to bacterial infection but not to viral infection. A clinical picture resembling rheumatoid arthritis develops in many. Before antibiotics, death occurred in the first decade. In the more usual X-linked form of the disease, plasma cells are lacking. A rarer form of agammaglobulinemia (Hitzig, W. H et al. 1961, Med. Wschr., 91:1625), which is inherited as an autosomal recessive, shows marked depression of the circulating lymphocytes, and lymphocytes are absent from the lymphoid tissue.
Mensink et al. (Clin. Genet., 1987, 31:91) mapped XLA to Xg21.3-q22. Schwaber (Clin. Invest., 1992, 89:2053) presented direct evidence that of a failure of V(D)J
recombination which causes arrest in the transition from pre-B cell to B lymphocyte. XLA patients have been classified in 2 general groups: those presenting at an early age with particularly severe infections and those with less severe disease in which production of immunoglobulin is sustained at low-to-normal levels well into the first decade of life. In the latter cases, an oncogenetic change may occur in which the defective tyrosine kinase no longer can sustain the B-cell population, and a progressive reduction in immunoglobulin production occurs (Ohta, Yet al., Proc. Nat. Acad. Sci., 1994, 91:9062) [0356] Btk is an inhibitor of the Fas/APO-1 death inducing signaling complex (DISC) in B-lineage lymphoid cells (Vassilev, A., et al., 1998, J. Biol. Chem., 274:1646-1656).
Additionally, Btk prevents ceramide- and vincristine-induced apoptosis. The fate of leukemia/lymphoma cells may reside in the balance between the opposing proapoptotic effects of caspases activated by DISC and an upstream anti-apoptotic regulatory mechanism involving Btk and/or its substrates (Vassilev, A., et al., 1998, J.
Biol. Chem. 274:1646-1656).

[0357] Accordingly, inhibitors of Btk are likely to enhance the drug sensitivity of B-lineage (e.g.
leukemia/lymphoma such as acute lymphocytic leukemia) cells. Thus, pharmacological agents with Btk-modulatory activity can be used as chemosensitizing agents for treating Btk-expressing malignancies or diseases caused by proliferation and antibody production of BTK-expressing B-cells, and as B-cell reconstituting agents in humoral immunodeficiencies with decreased numbers or absence of B-cells. Furthermore, Btk modulating agents are useful as immunosuppressive agents for prevention of hyperacute rejection of organs in transplantation, which is directed by B-cells, autoimmune diseases, and conversion of immunity to drugs (e.g. antibodies or biologicals) or blood products (e.g. coagulation factors such as Factor VIII) in patients who develop antibodies to such agents.

[0358] Significant additional research has defined the role of Btk in the cell. For example, Cheng et al. (Proc. Nat. Acad. Sci., 1994, 91:8152) showed that Btk interacts with the SH3 domains of Fyn, Lyn, and Hck, all of which are activated upon stimulation of B- and T-cell receptors. These findings extended the range of interactions mediated by SH3 domains and provide indication of a link between Btk and previously established signaling pathways in B lymphocytes. Further, linkage studies involving 1,114 progeny backcross revealed colocalization of X-linked immunodeficiency (xid) mutation in mice with the BTK gene for (Thomas, J. D. et al., Science 1993, 261:355). And further, Uckun et al. (Uckun, F. M et al. 1996, Science 273: 1096) reported that DT-40 lymphoma B cells rendered Btk deficient through targeted disruption of the BTK gene did not undergo radiation-induced apoptosis. Finally, Btk plays a key role in endotoxin-induced TNFa release from monocytes (Horwood, N.J. et al., J. Exp. Med. 2003, 197:1603).

[0359] Btk inhibitors may be useful in treating multiple sclerosis, systemic lupus erythematosis, rheumatoid arthritis, and Graves' disease, in addition to the diseases discussed above.

[03601 Cyclin dependent kinases: The cyclin dependent kinases (Cdk) play a major role in the signaling of cell cycles. The Cdk binds cyclin protein to form complex involved in the various stages of the cell cycle. Aberrant cell cycle progression occurs in cancers, often involving the activity of Cdk, such that inhibitors of Cdk are potential anti-cancer therapeutics.

[03611 Cdk2: Target kinase Cdk2 (i.e., Cyclin dependent kinase 2) is a 33.9 kDa serine/threonine kinase (STK) encoded by chromosome 12g13 (symbol: CDK2). Cdk2 is also known as p33 protein kinase, and cell division protein kinase 2. De Bondt et al. reported the X-ray crystallographic structure of Cdk2 (De Bondt et al., Nature 1993, 363: 595-602). Cdk2 is involved in control of the human cell cycle. Cdk2 activation requires association with cyclins and leads to cell proliferation.
Further, inhibition of cellular proliferation occurs upon association of inhibitors (e.g., cyclin-dependent kinase inhibitor 1B) with the cyclin-Cdk2 complex. Cyclin E/Cdk2 complexes play a role in carcinogenesis, for example, Cdk2 amplication is associated with concurrent Cyclin E
amplification in several cancers, including colorectal and ovarian cancer (Kitahara et al., Int. J.
Cancer, 1995, 53: 1986-1989; Marone et al., Int. J. Cancer, 1998, 75: 31-39).
Teixeira et al. show that retinoic acid inhibits cell cycle progression of MCF-7 human breast cancer cells by inhibiting cdk2 mRNA and protein production and by decreasing cdk2 activity (Teixeira et al., Mol Endocrinol 1997, 11(9):1191-202). Cipriano and Chen studied the TGF-betal effect on normal human prostate and carcinoma cells, and showed that normal cells were sensitive to growth inhibition, whereas tumor cells were not or only minimally inhibited regardless of the concentration of TGF-betal, and correlated these results to Cdk2 activity. Their results indicate that a lack of inhibition of the Cdk2 activity correlates with insensitivity to TGF-betal in prostate tumor cells (Cipriano and Chen, Oncogene 1998, 17(12):1549-56). Cdk2 inhibitors may be useful in treating cancer, including prostate, breast, colorectal and ovarian cancer.

[03621 Cdk4: Target kinase Cdk4 (i.e., Cyclin dependent kinase 4) is a 33 kDa STK encoded by chromosome 12g14 (symbol: CDK4). Lam et al. reported expression of CDK4 and CDK6 was elevated relative to matched normal brain tissue in eight of 18 glioblastoma multiforme (GBM) tumours (44%). Their data attests to the functional importance of both CDK4 and CDK6 in astrocytic tumourigenesis, particularly during the later stages of tumour progression (Lam et al., Br J Neurosurg 2000, 14(1):28-32). Backlund et al. found that loss of both wild-type copies of any of the three tumour suppressor genes CDKN2A, CDKN2B and RB1 or gene amplification of CDK4, disrupting the Rbl pathway, were associated with shorter survival in anaplastic astrocytoma patients (Backlund et al., Br J Cancer 2005, 93(1):124-30). Yu et al. report that the ability of cyclin D1 to activate cyclin-dependent kinase CDK4 underlies the critical role for cyclin D1 in breast cancer formation. They also found that the continued presence of CDK4-associated kinase activity is required to maintain breast tumorigenesis (Yu et al., Cancer Cell 2006, 9(1):23-32). Cdk4 inhibitors may be useful in treating cancer, including glioblastoma (e.g. glioblastoma multiforme), anaplastic astrocytoma, and breast cancer.

[0363] Cdk5: Target kinase Cdk5 (i.e., Cyclin dependent kinase 5) is a 33.3 kDa STK encoded by chromosome 7q36 (symbol: CDK5). Cruz et al. state that proteolytic cleavage of p35 generates p25, leading to aberrant Cdk5 activation. The accumulation of p25 is implicated in several neurodegenerative diseases. Their findings provide compelling evidence that in vivo deregulation of Cdk5 by p25 plays a causative role in neurodegeneration and the development of neurofibrillary pathology (Cruz et al., Neuron 2003, 40(3):471-83). Takahashi et al.
investigated the Cdk5 distribution pattern in diffuse Lewy body disease brains using immunohistochemistry. Their data suggest that Cdk5 may be associated with Lewy body formation (Takahashi et al., Brain Res 2000, 862(1-2):253-6). Cdk5 inhibitors may be useful in treating neurodegenerative disorders, including Alzheimer's disease, amyotrophic lateral sclerosis and Lewy body disease.

[0364] Cdk6: Target kinase Cdk6 (i.e,, Cyclin dependent kinase 6) is a 36.9 kDa STK encoded by chromosome 7g21-q22 (symbol: CDK6). Lam et al. reported expression of CDK4 and CDK6 was elevated relative to matched normal brain tissue in eight of 18 glioblastoma multiforme (GBM) tumours (44%). Their data attests to the functional importance of both CDK4 and CDK6 in astrocytic tumourigenesis, particularly during the later stages of tumour progression (Lam et al., Br J Neurosurg 2000, 14(1):28-32). Costello et al., applied restriction landmark genomic scanning to matched samples of glioma and normal brain DNA and found tumor-specific amplification of the gene encoding cyclin-dependent kinase 6 (CDK6). They also corroborated this finding by identifying both amplification-associated and amplification-independent increases in CDK6 protein levels in gliomas relative to matched normal brain samples (Costello et al., Cancer Res 1997, 57(7):1250-4). Corcoran et al. found in two samples from patients with splenic lymphoma with villous lymphocytes (SLVL), the CDK6 protein was markedly over expressed. They suggest that dysregulation of CDK6 gene expression contributes to the pathogenesis of SLVL and splenic marginal zone lymphoma (SMZL) (Oncogene 1999, 18(46):6271-7). Chilosi et al. provide evidence that CDK6 is abnormally expressed in T-cell lymphoblastic lymphoma/leukemia (T-LBL/ALL) and may be involved in the pathogenesis of this malignancy (Chilosi et al., Am J Pathol 1998, 152(l):209-17). Cram et al. show that Indole-3-carbinol (13C) can induce a G(1) cell cycle arrest of human MCF-7 breast cancer cells that is accompanied by the selective inhibition of cyclin-dependent kinase 6 (CDK6) expression (Cram et al., J Biol Chem 2001, 276(25):22332-40). Cdk6 inhibitors may be useful in treating cancer, including glioblastoma multiforme, non-Hodgkin's lymphoma, splenic marginal zone lymphoma, T-cell lymphoblastic lymphoma (T-LBL) and T-cell acute lymphoblastic leukemia (T-ALL).

[0365] CHK1: Target kinase Chkl (i.e., Checkpoint kinase) is a 54.4 kDa STK
encoded by chromosome 11g24 (symbol: CHEK1, CHK1). CHKI is involved in DNA damage checkpoint.

Carassa et al., to understand the role of Chkl and Chk2 in the cellular response to different anticancer agents, knocked down the expression of each protein or simultaneously of both proteins by using the small interfering RNA technique in a HCT-l 16 colon carcinoma cell line and in its isogenic systems in which p53 and p21 had been inactivated by targeted homologous recombination. They show that inhibition of Chkl but not of Chk2 in p21(-/-) and p53(-I-) cells caused a greater abrogation of G(2) block induced by ionizing radiation and cis-diamine-dichloroplatinum treatments and a greater sensitization to the same treatments than in the parental cell line with p53 and p21 wild type proteins.
Their data further emphasise the role of Chkl as a molecular target to inhibit in tumors with a defect in the G(1) checkpoint with the aim of increasing the selectivity and specificity of anticancer drug treatments (Carrassa et al., Cell Cycle 2004, 3(9):1177-81). Similarly, studies by Hirose et al. focused on the mechanism by which Temozolomide (TMZ) induces G(2)-M arrest and on whether inhibition of such G(2)-M arrest might sensitize glioma cells to TMZ-induced toxicity.
U87MG glioma cells treated with TMZ underwent G(2)-M arrest associated with Chkl activation and phosphorylation of both cdc25C and cdc2. These TMZ-induced effects were inhibited by the Chkl kinase inhibitor UCN-01. Although not in itself toxic, UCN-01 increased the cytotoxicity of TMZ
5-fold, primarily by inhibiting cellular senescence and increasing the percentage of cells bypassing G(2)-M arrest and undergoing mitotic catastrophe. In addition to enhancing TMZ-induced cytotoxicity in p53-proficient cells, UCN-01 also blocked TMZ-induced Chkl activation and transient G(2)-M
arrest in p53-deficient U87MG-E6 cells and similarly enhanced TMZ-induced mitotic catastrophe and cell death.
Taken together, their results indicate that Chkl links TMZ-induced DNA
mismatch repair to G(2)-M
arrest. Furthermore, inhibition of the cytoprotective G(2) arrest pathway sensitizes cells to TMZ-induced cytotoxicity and may represent a novel, mechanism-based means of increasing TMZ efficacy in both p53 wild-type and p53 mutant glioma cells. (Hirose et al., Cancer Res 2001, 61(15):5843-9).
As such, CHK1 inhibitors may be used in combination therapy to improve the therapeutic efficacy of chemotherapeutic drugs. CHK1 inhibitors may be useful in combination with chemotherapeutic drugs in treating cancer.

[03661 Csk: Target kinase Csk (i.e., c Src kinase) is a 50.7 kDa tyrosine kinase encoded by chromosome 15g23-q25 (symbol: CSK). Csk, cloned by Partanen et al. (Oncogene 1991, 6: 2013-2018), is a cytoplasmic tyrosine kinase that downregulates the tyrosine kinase activity of the Src oncoprotein through tyrosine phosphorylation of the Src carboxy terminus.
Activation of Csk may be therapeutic for cancers in which Src is activated, such as in colon and pancreatic carcinomas (Lutz et al., Biochem Biophys Res Commun 1998, 243(2):503-8; Cam et al., Cancer 2001, 92(l):61-70).
Zheng and She state that the lymphoid-specific phosphatase (LYP) encoded by PTPN22 is involved in preventing spontaneous T-cell activation by dephosphorylating and inactivating T-cell receptor-associated Csk kinase. They genotyped 396 type 1 diabetic patients and 1,178 control subjects of Caucasian descent from north central Florida and report a strong association between type 1 diabetes and a polymorphism (R620W) in the PTPN22 gene. In vitro experiments have shown that the mutant 620W LYP protein (1858T) does not bind Csk. Together with previous reports of the association between PTPN22 and type 1 diabetes, as well as rheumatoid arthritis and systemic lupus erythematosus, these results provide compelling evidence that LYP is a critical player in multiple autoimmune disorders (Zheng and She, Diabetes 2005, 54(3):906-8). Csk modulators, including inhibitors, may be useful in treating autoimmune diseases, including type 1 diabetes, rheumatoid arthritis and systemic lupus erythematosus.

[0367] EGFR: Target kinase EGFR (i.e., Epidermal Growth Factor Receptor) is a 134.3 kDa transmembrane tyrosine kinase coded by chromosome 7p12.3-p12.1 (symbol: EGFR).
OMIM
indicates that EGF enhances phosphorylation of several endogenous membrane proteins, including EGFR. EGFR has 2 components of different molecular weight; both contain phosphotyrosine and phosphothreonine but only the higher molecular weight form contains phosphoserine (Carlin and Knowles, Proc. Nat. Acad. Sci. 1982, 79: 5026-5030.). Carlin et al.
(Cytogenet. Cell Genet. 1982, 32:
256) showed that the specific cell surface antigen previously called SA7 (Aden and Knowles, Immunogenetics 1976, 3: 209-211) is identical to EGFR. EGFR signaling involves small GTPases of the Rho family, and EGFR trafficking involves small GTPases of the Rab family.
Lanzetti et al.
(Nature 2000, 408: 374-377) reported that the EPS8 protein connects these signaling pathways. EPS8 is a substrate of EGFR that is held in a complex with SOS 1 by the adaptor protein E3B 1, thereby mediating activation of RAC. Through its SH3 domain, EPS8 interacts with RNTRE. Further, Lanzetti et al. (ibid) showed that RNTRE is a RAB5 GTPase-activating protein whose activity is regulated by EGFR. By entering in a complex with EPS8, RNTRE acts on RAB5 and inhibits internalization of EGFR. Furthermore, RNTRE diverts EPS8 from its RAC-activating function, resulting in the attenuation of RAC signaling. Thus, depending on its state of association with E3B1 or RNTRE, EPS8 participates in both EGFR signaling through RAC and EGFR
trafficking through RAB5 (OMIM MIM Number: 131550: 12/16/2005).

[0368] EGFR is implicated in breast cancer, colorectal, and bladder cancer, and modulation of EGFR activity is a therapeutic route to amelioration of these pathologic states (X-u et al., Mol Cancer Ther 2005, 4(3):435-42). An important unmet need has emerged in non small cell lung cancer patients who initially respond to treatment with EGFR inhibitors but then develop resistance to the initial drug (Koboyashi, S. et al. N Engl J Med. 2005, 352:786-92). EGFR is also a possible target for treating glioblastoma multiforme (Raizer, J Neurooncol 2005, 74(1):77-86), and squamous cell carcinomas, for example in the esophagus (Hanawa et al., hit J Cancer 2006, 118(5):1173-80), head and neck (Hambek et al., Anticancer Res 2004, 24(6):3881-6), and oral cavity and tongue (Ekberg et al., hit J Oncol 2005, 26(5):1177-85). Unlu and Leake studied the effect of epidermal growth factor (EGF) and a specific inhibitor of EGFR, on the growth and invasiveness of the prostate cancer cell lines PC3 and DU145. Their results indicate that EGF is a potent stimulative agent for both growth and invasion in prostate cancer cells, and that targeting the EGFR function inhibits not only tumor growth but also invasiveness (Unlu and Leake, Int J Biol Markers 2003, 18(2):139-46). EGFR
inhibitors may be useful in treating cancer, including breast, colon, bladder, prostate and non small cell lung cancer, squamous cell carcinomas of the head and neck, oral cavity, and esophagus, and glioblastoma multiforme.

[0369] EphAl: Target kinase EphAl (i.e., Ephrin Receptor Al) is a 108.1 kDa tyrosine kinase encoded by chromosome 7q32-q34 (symbol: EPHAl). OMIM indicates that the EPH
and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases. They have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the Eph subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ligands for Eph receptors have been named "ephrins"
by the Eph Nomenclature Committee (Cell 1997, 90: 403-404). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. The Eph Nomenclature Committee (ibid.) proposed that Eph receptors interacting preferentially with ephrin-A proteins be called EphA and Eph receptors interacting preferentially with ephrin-B proteins be called EphB. Mara et al. (1988) reported characterization of the novel receptor tyrosine kinase gene, EPH. The splicing points of kinase domain-encoding exons were completely distinct from those of other protein tyrosine kinase genes, suggesting that this is the earliest evolutionary split within this family. In Northern blot analysis, EPH gene mRNA was detected in liver, lung, kidney, and testes of rat; screening of 25 human cancers of various cell types showed preferential expression in cells of epithelial origin. Overexpression of EPH
mRNA was found in a hepatoma and a lung cancer without gene amplification. Southern blot analysis of DNAs from human-mouse hybrid clones with an EPH probe showed that this gene is present on human chromosome 7. Two other receptor tyrosine kinase genes, MET and EGFR, are on the same chromosome. By in situ hybridization, Yoshida et al. (1989) assigned the EPH
locus to 7q32-q36.
Although ephrins form a high-affinity multivalent complex with their receptors present on axons, axons can be rapidly repelled rather than being bound (OMIM MIM Number:
179610: 9/5/2000). Lin et al. identified 5 PTKs that were overexpressed in head and neck squamous cell carcinoma (HNSCC) using a reverse transcriptase-PCR technique and confirmed the overexpression of 3 known PTKs in some of the 8 archival HNSCC specimens studied. Their finding suggests that the signaling pathways mediated through EphAl, Brk, and Ron may be involved in the development and progression of HNSCC (Lin et al., Arch Otolaryngol Head Neck Surg 2004, 130(3):311-6). EphAl inhibitors may be useful in treating cancer, including liver and lung cancer, and head and neck squamous cell carcinoma.

[03701 EphA2: Target kinase EphA2 (i.e., Ephrin Receptor A2) is a 108.3 kDa tyrosine kinase encoded by chromosome lp36.1 (symbol: EPHA2). EphA2, similar to other ephrin receptors, is found in epithelial, lymphoid, and especially neuron tissue where EphA2 is critically involved in short-range contact-mediated axonal guidance. Further, EphA2 is highly expressed in metastatic melanoma cells. Ephrin Al, a ligand for EphA2, was shown to be up regulated during melanoma progression (Easty et al., Int J Cancer 1999, 84(5):494-501). Hattori et al.
showed that ephrin-A2 forms a stable complex with the metalloproteinase Kuzbanian, involving interactions outside the cleavage region and the protease domain. Eph receptor binding triggered ephrin-A2 cleavage in a localized reaction specific to the cognate ligand. The cleavage-inhibiting mutation in ephrin-A2 delayed axon withdrawal. Hattori et al. (ibid) concluded that their studies reveal mechanisms for protease recognition and control of cell surface proteins, and, for ephrin-A2, they may provide a means for efficient axon detachment and termination of signaling (Hattori et al., Science 2000, 289:
1360-1365). Ireton and Chen review EphA2 as a promising target for cancer therapeutics, indicating EphA2 is overexpressed in breast, prostate, lung, and colon cancers (Ireton and Chen, Curr Cancer Drug Targets 2005, 5(3):149-57). Landen et al. state that EphA2 is involved in many processes crucial to malignant progression, such as migration, invasion, metastasis, proliferation, survival and angiogenesis. Inducing EphA2 downregulation by any one of several mechanisms (antibody-mediated inhibition of signalling, antibody-mediated downregulation of total EphA2 expression and siRNA-mediated inhibition of expression) has been shown to decrease tumour growth, prolong survival and inhibit angiogenesis in multiple preclinical models of ovarian, breast and pancreatic cancer. Targeting EphA2 is especially attractive in ovarian cancer, in which overexpression is present in > 75% of cases (Landen et al., Expert Opin Ther Targets 2005, 9(6):1179-87). Abraham et al, state that the clinical significance of the expression of EphA2 was observed in breast, prostate, colon, skin, cervical, ovarian, and lung cancers. They studied EphA2 to determine the expression of EphA2 and its ligand, Ephrin A-1, and E-cadherin in carcinoma of the urinary bladder, and determine EphA2 as a new target for therapy in bladder cancer. They conclude EphA2 may serve as a novel target for bladder cancer therapy, (Abraham et al., Clin Cancer Res 2006, 12(2):353-60;).
EphA2 inhibitors may be useful in treating cancer, including bladder, breast, prostate, colon, skin, cervical, ovarian, pancreatic, and lung cancer and melanoma.

[03711 EphB2: Target kinase EphB2 (i.e., Ephrin Receptor B2) is a 117.5 kDa transmembrane tyrosine kinase encoded by chromosome lp36.1-p35 (symbol: EPHB2). Mann et al.
state that forward and reverse signaling mediated by EphB tyrosine kinase receptors and their transmembrane ephrin-B ligands play important roles in axon pathfinding. In their investigations of growth cones from the ventral (EphB receptor-bearing) and dorsal (ephrin-B-bearing) embryonic Xenopus retina designed to investigate the signaling mechanisms in both forward and reverse directions, it is reported that unclustered, but not clustered, EphB2 ectodomains trigger fast (5-10 min) transient collapse responses in growth cones. This collapse response is mediated by low levels of intracellular cyclic GMP and requires proteasome function. In contrast, clustered, but not unclustered, ephrin-B 1 ectodomains cause slow (30-60 min) growth cone collapse that depends on high cGMP levels and is insensitive to inhibition of the proteasomal pathway. Upon receptor-ligand binding, endocytosis occurs in the reverse direction (EphB2-Fc into dorsal retinal growth cones), but not the forward direction, and is also sensitive to proteasomal inhibition. Endocytosis is functionally important because blocking of EphB2 internalization inhibits growth cone collapse. They state their data reveals that distinct signaling mechanisms exist for B-type Eph/ephrin-mediated growth cone guidance and suggest that endocytosis provides a fast mechanism for switching off signaling in the reverse direction (Mann et al., J Neurobiol 2003, 57(3):323-36). Nakada et al. demonstrate that migrating glioblastoma cells overexpress EphB2 in vitro and in vivo; glioma migration and invasion are promoted by activation of EphB2 or inhibited by blocking EphB2. Dysregulation of EphB2 expression or function may underlie glioma invasion (Nakada et al., Cancer Res 2004, 64(9):3179-85).
Wu et al., investigated the expression of EphB2 and EphB4 in breast carcinomas.
Clinicopathological and survival correlations were statistically analyzed in a series of 94 breast carcinomas, 9 normal specimens and 4 breast carcinoma cell lines. Both EphB2 and EphB4 RTPCR
products could be detected in all specimens. Increased EphB2 protein expression was negatively associated with overall survival (Wu et al., Pathol Oncol Res 2004, 10(1):26-33). Hafner et al.
studied expression profiles of 12 different Eph receptors and 8 ephrins inhuman lung, colorectal, kidney, liver, and brain cancers.
They report EphB2 was up-regulated 9-fold in hepatocellular carcinoma (Hafner et al., Clinical Chemistry 2004, 50:490-99). Umeda et al. studied the expression of ephrinB2 and EphB receptors within fibroproliferative membranes in patients with ocular angiogenic diseases collected during vitrectomy. EphB2 and EphB3 expression was observed on fibroproliferative membranes that were harvested from patients with proliferative diabetic retinopathy (EphB2, 90.0%;
EphB3, 70.0%) and retinopathy of prematurity (EphB2, 35.0%; EphB3, 45.0%). Their data suggest that the ephrinB2-EphB2/B3 system may play an important role in ocular angiogenesis (Umeda et al., Am J Ophthalmol 2004, 138(2):270-9). EphB2 inhibitors may be useful in treating cancer, including breast cancer, hepatocellular carcinoma and glioblastoma, and for use in treating ocular angiogenesis diseases, including retinopathy (e.g. retinopathy of prematurity and proliferative diabetic retinopathy).

[0372] EphB4: Target kinase EphB4 (i.e., Ephrin Receptor B4) is a 108.3 kDa transmembrane tyrosine kinase encoded by chromosome 7q22 (symbol: EPHB4). EphB4 belongs to the Eph family of receptor tyrosine kinases. Developmental studies have shown that the Eph receptors, by regulating cell adhesion and cell movement in the embryo, are important for the proper organization and integrity of tissues. Because tissue disorganization and abnormal cell adhesion, movement, and survival characterize the more advanced stages of cancer, the inappropriate functioning of an Eph receptor in breast tumor cells enhances malignancy. Xia et al. studied the biological function of the receptor tyrosine kinase EphB4 in bladder cancer. All of nine bladder cancer cell lines examined expressed EphB4. Further, they showed EphB4 knockdown using specific siRNA and antisense oligodeoxynucleotides molecules led to a profound inhibition in cell viability associated with apoptosis via activation of caspase-8 pathway and downregulation of antiapoptotic factor, bcl-xl.
Furthermore, EphB4 knockdown significantly inhibited tumor cell migration and invasion. EphB4 knockdown in an in vivo murine tumor xenograft model led to a nearly 80%
reduction in tumor volume associated with reduced tumor proliferation, increased apoptosis and reduced tumor microvasculature (Xia et al., Oncogene 2006, 25(5):769-80). Xia et al. also studied the expression and biological role of EphB4 in prostate cancer. They found EphB4 mRNA is expressed in 64 of 72 (89%) prostate tumor tissues assessed and EphB4 protein expression is found in the majority (41 of 62, 66%) of tumors, and 3 of 20 (15%) normal prostate tissues. They also showed knockdown of the EphB4 protein using EphB4 short interfering RNA or antisense oligodeoxynucleotide significantly inhibits cell growth/viability, migration, and invasion, and induces apoptosis in prostate cancer cell lines (Xia et al., Cancer Res 2005, 65(11):4623-32). Lee et al. used RT-PCR, western blotting and immunohistochemical techniques to examine EphB4 expression and protein levels in human prostate cancer cell lines LNCaP, DUI 45 and PC3. Immunohistochemistry was also used to examine localisation of EphB4 in tissue samples from 15 patients with prostate carcinomas. All three prostate cancer cell lines expressed the EphB4 gene and protein. EphB4 immunoreactivity in vivo was significantly greater in human prostate cancers as compared with matched normal prostate epithelium and there appeared to be a trend towards increased expression with higher grade disease (Lee et al., BMC Cancer 2005, 5:119). Stephenson et al. used commercially available cDNA
arrays to identify EphB4 as a gene that is up-regulated in colon cancer tissue when compared with matched normal tissue from the same patient (Stephenson et al., BMC Mol Biol 2001, 2:15).
Takai et al. used fluorescent immunohistochemistry to analyze serial frozen sections of 20 endometrial carcinomas and 20 normal endometria for EphB4 and ephrin-B2 protein expression. Further, they analyzed the relationship between the patient's characteristics and the percentages of EphB4- and ephrin-B2-stained cells. They indicate the results demonstrate that increased EphB4 and ephrin-B2 expression may reflect or induce in endometrial carcinomas increased potential for growth and tumorigenicity (Takai et al., Oncol Rep 2001, 8(3):567-73). Sinha et al., studied expression of EphB4 in six men with primary squamous cell carcinoma of the head and neck (HNSCC) that had metastasized to the cervical lymph nodes. They obtained specimens of the primary tumor, the nodal metastasis, and the adjacent normal mucosa, and performed immunocytochemistry on each. They observed EphB4 expression in all primary and metastatic tumors and no expression in the normal tissue. In each of the six patients, expression was greater in the metastatic tumor than in the primary tumor (Sinha et al., Ear Nose Throat J 2003, 82(11):866, 869-70, 887). EphB4 inhibitors maybe useful in treating cancer, including breast, bladder, prostate, colon, and endometrial cancers and head and neck squamous cell carcinoma.

[0373] Erk2: Target kinase Erk2 (i.e., extracellular signal-regulated kinase 2) is a 41.4 kDa dual function serine/threonine-tyrosine kinase encoded by chromosome 22g11.2 (symbol: MAPK1). Erk2 is a member of the mitogen-activated protein (MAP) kinase family and is alternatively known as mitogen-activated protein kinase 1 (i.e., MAPK1). MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development.

[0374] The activation of Erk2 requires phosphorylation by upstream kinases.
Upon activation, Erk2 translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets, in addition to other targets including microtubule associated protein 2, myelin basic protein and ELK1.
MacKenzie et al. state that the cAMP-specific phosphodiesterase family 4, subfamily D, isoform 3 (i.e., PDE4D3) is shown to have FQF (i.e., Phe-Gln-Phe) and KIM (i.e., Kinase Interaction Motif) docking sites for Erk2. These sites straddle the Ser(579) target residue for Erk2 phosphorylation of PDE4D3. Mutation of either or both of these docking sites prevent Erk2 from being co-immunoprecipitated with PDE4D3, ablate the ability of epidermal growth factor (EGF) to inhibit PDE4D3 through Erk2 action in transfected COS cells, and attenuate the ability of Erk2 to phosphorylate PDE4D3 in vitro. The two conserved NH(2)-terminal blocks of sequence, called upstream conserved regions 1 and 2 (i.e., UCRI and UCR2), that characterize PDE4 long isoforms, are proposed to amplify the small, inherent inhibitory effect that Erk2 phosphorylation exerts on the PDE4D catalytic unit. In contrast to this, the lone intact UCR2 region found in PDE4D1 directs COOH-terminal Erk2 phosphorylation to cause the activation of this short isoform. From the analysis of PDE4D3 truncates, it is suggested that UCR1 and UCR2 provide a regulatory signal integration module that serves to orchestrate the functional consequences of Erk2 phosphorylation. The PDE4D
gene thus encodes a series of isoenzymes that are either inhibited or activated by Erk2 phosphorylation and thereby offers the potential for ERK2 activation either to increase or decrease cAMP levels in cellular compartments (MacKenzie et al., J Biol Chem 2000, 275(22):16609-17).
[0375] According to OMIM, Pleschka et al. (Nature Cell Biol., 2001, 3: 301-305) proposed that Erk2 regulates a cellular factor involved in the viral nuclear export protein function. They suggested that local application of MEK inhibitors may have only minor toxic effects on the host while inhibiting viral replication without giving rise to drug-resistant virus variants (OMIM MIM Number:
176948: 10/27/2005). Erk2 is involved in cytokine signaling and is a target for treating inflammation.
Ramesh and Philipp state that lipoproteins are the key inflammatory molecule type of Borrelia burgdorferi, the spirochete that causes Lyme disease. They investigated whether specific inhibition of p.su ana trK1/z IVIAr& wouia mninit iivr-aipna ana u,-o proaucuuii auu Lnus astrocyte apoptosis, and proliferation, respectively. Lipoprotein-stimulated IL-6 production was unaffected by the MAPK
inhibitors. In contrast, inhibition of both p38 and Erkl/2 significantly diminished TNF-alpha production, and totally abrogated production of this cytokine when both MAPK
pathways were inhibited simultaneously. MAPK inhibition thus may be considered as a strategy to control inflammation and apoptosis in Lyme neuroborreliosis (Ramesh and Philipp, Neurosci Lett 2005, 384(1-2):112-6). The role of Erk2 in signaling of cell differentiation, proliferation and survival suggests that inhibition of Erk2 may be therapeutic for several types of cancer. Husain et al. studied the effect of NSAIDs on MAPK activity and phosphorylation in gastric cancer.
They conclude that NS-398 (a selective COX-2 inhibitor) and indomethacin (a non-selective NSAID) significantly inhibit proliferation and growth of human gastric cancer cell line MKN28. This effect is mediated by NSAID-induced inhibition of MAPK (ERK2) kinase signaling pathway, essential for cell proliferation (Husain et al., Life Sci 2001, 69(25-6):3045-54). Erk2 inhibitors may be useful in treating cancer, including gastric cancer, and in treating inflammation, including control of inflammation and apoptosis in Lyme neuroborreliosis.

[0376] Fak: Target kinase Fak (i.e., Focal adhesion kinase 1, aka protein tyrosine kinase 2, PTK2) is a 119.2 kDa tyrosine kinase encoded by chromosome 8q24.3 (symbol: PTK2).
The structure of Fak comprises a B41 (i.e., Band 4.1 homology) domain in addition to the TK domain.
Fak and a related protein Pyk21CAK-beta are cytoplasmic non-receptor protein tyrosine kinases.
Localization of Fak via its C-terminal Focal Adhesion Targeting domain to focal complexes/adhesions (sites of integrin receptor clustering) is a prerequisite for Fak activation. Auto- or trans-phosphorylation of Fak on Tyr397 allows for docking of the Src family kinases, among other molecules.
Src kinases phosphorylate Fak not only on the Tyr residues in the activation loop of the FAK kinase, but also create binding sites for downstream signaling components. Moreover, FAK can be activated by other receptors, linking them to the integrin signaling pathway. Activation of FAK
can promote cell spreading, locomotion, survival and anchorage-dependent growth.

[0377] FAK-related non-kinase (FRNK), the C-terminal portion of FAK is expressed in some cell types by activating transcription from an alternative promoter. FRNK is proposed to function as an endogenous inhibitor of Fak signaling.

[0378] The role of Fak in signaling of cell proliferation, migration, adhesion and survival may be targeted in therapeutics for several types of cancer. Lightfoot et al. used immunohistochemical techniques to assess FAK expression in patients with fibrocystic disease (FCD), atypical ductal hyperplasia (ADH), ductal carcinoma in situ (DCIS) and infiltrating ductal carcinoma (IDC). The pattern of FAK expression in DCIS was significantly higher than ADH (p <
0.0001) and IDC (p =
0.02). They conclude that FAK overexpression in preinvasive, DCIS tumors precedes tumor cell invasion or metastasis, suggesting that FAK may function as a survival signal and be an early event in breast tumorigenesis (Lightfoot et al., Breast Cancer Res Treat 2004, 88(2):109-16). Miyazaki et al.
performed an immunohistochemical analysis of FAK protein expression to determine the relationship between FAK overexpression and clinicopathological factors in oesophageal squamous cell carcinoma (ESCC). They concluded that FAK overexpression of ESCC was related to cell differentiation, tumour invasiveness, and lymph node metastasis. Consequently, patients with ESCC
who had FAK overexpression had a poor prognosis (Miyazaki et al., Br J Cancer 2003, 89(l):140-5).
Smith et al. tested antisense oligonucleotide inhibitors of FAK, in combination with 5-fluorouracil (5-FU), to increase its sensitivity in human melanoma cell lines. They conclude their data show that the downregulation of FAK by antisense oligonucleotide combined with 5-FU
chemotherapy results in a greater loss of adhesion and greater apoptosis in melanoma cells than treatment with either agent alone, suggesting that the combination may be a potential therapeutic agent for human melanoma in vivo (Smith et al., Melanoma Res 2005, 15(5):357-62). In a review, Natarajan et al. summarize data that has demonstrated 1) elevated FAK expression in anaplastic astrocytoma and glioblastoma tumor biopsy samples, 2) a role for FAK in the promotion of glioblastoma cell proliferation, survival and migration in vitro, and 3) a role for FAK in the promotion of glioblastoma cell proliferation in vivo in an animal model (Natarajan et al., Cancer J 2003, 9(2):126-33). Rovin et al.
investigated FAK
expression in human prostate specimens by using immunohistochemistry. In their conclusion, they suggest that the sustained elevated levels of FAK expression during prostate tumor cell progression is consistent with a role for FAK in the development and maintenance of prostate carcinoma (Rovin et al., Prostate 2002, 53(2):124-32). Itch et al. investigated whether focal adhesion kinase (FAK) is involved in the progression of human hepatocellular carcinoma (HCC). They conclude their data suggests that FAK plays an important role in promoting tumor progression, especially vascular invasion, in HCC (Itch et al., Clin Cancer Res 2004, 10(8):2812-7). von Sengbusch et al. state that organ-specific tumor cell adhesion to extracellular matrix (ECM) components and cell migration into host organs often involve integrin-mediated cellular processes that can be modified by environmental conditions acting on metastasizing tumor cells, such as shear forces within the blood circulation.
Since the focal adhesion kinase (FAK) appears to be essential for the regulation of the integrin-mediated adhesive and migratory properties of tumor cells, they investigated its role in early steps of the metastatic cascade using in vitro and in vivo approaches. They summarize that FAK appears to be involved in early events of integrin-mediated adhesion of circulating carcinoma cells under fluid flow in vitro and in vivo. This kinase may take part in the establishment of definitive adhesive interactions that enable adherent tumor cells to resist fluid shear forces, resulting in an organ-specific formation of distant metastases (von Sengbusch et al., Am J Pathol 2005, 166(2):585-96).

[0379] Westhoff et al., note that elevated expression of the nonreceptor tyrosine kinases Src and Fak correlates with malignancy potential and poor clinical prognosis in colon and breast tumors. They also state that recent studies monitoring focal adhesion dynamics in cells deficient for Fak and Src implicate Src and Fak as critical mediators of integrin adhesion turnover that promote cell migration.
Cells devoid of FAK exhibit impaired migration and have large peripheral focal adhesion structures, while cells lacking the three ubiquitous Src family members Src, Fyn, and Yes also demonstrate altered distribution of focal adhesions and impaired cell migration. Src kinase activity is clearly necessary for focal adhesion turnover and cell motility, presumably by tyrosine phosphorylation of key focal adhesion substrates, such as FAK. The extracellular regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) pathway is also important in regulating focal adhesion dynamics during cell motility and it is likely that ERK/MAPK contributes to Src-induced focal adhesion turnover. Further, Westhoff et al. have recently reported that ERK/MAPK, which is recruited to focal adhesions following v-Src activation, is required for maximal activity of the protease calpain 2 promoting focal adhesion turnover and migration of v-Src-transformed cells.
ERKIMAPK-induced activation of calpain 2 is also required for epidermal growth factor-induced substrate deadhesion and cell motility. Six major tyrosine phospho-acceptor sites have been identified on Fak, at positions 397, 407, 576, 577, 861, and 925. Tyr 397 becomes phosphorylated (presumed by auto-phosphorylation) upon integrin engagement. This leads to the formation of a consensus binding site for the Src SH2 domain, promoting association between Src and FAK. Phosphorylation of the remaining tyrosine residues on Fak is considered to be Src-dependent. Westhoff et al., generated a FAK mutant (4-9F-Fak) in which each of the putative Src-induced tyrosine phosphorylation sites (Tyr 407, 576, 577, 861, and 925) has been mutated to a phenylalanine (Phe). They found that v-Src-induced phosphorylation of FAK on tyrosine residues is necessary to enhance the adaptor function of Fak with regard to assembly of the calpain 2/FAK/p42ERK complex. Src-induced phosphorylation of Fak is also required for Fak to undergo proteolytic cleavage by calpain in v-Src-transformed cells and is necessary for calpain-mediated focal adhesion turnover during transformation and cell migration. In addition, they show that Src-induced phosphorylation of Fak also regulates F-actin assembly and cell spreading. We further demonstrate a role for Src-induced tyrosine phosphorylation of Fak in survival and anchorage-independent growth of transformed cells (Westhoff et al., Molecular and Cellular Biology, 2004, 24: 8113-8133).

[0380] Fak inhibitors may be useful in treating cancer, including colon and breast cancers, melanoma, ductal carcinoma in situ, oesophageal squamous cell carcinoma, anaplastic astrocytoma and glioblastoma, and human hepatocellular and prostate carcinomas, as well as in reducing tumor metastasis. They may also be used in combination with other chemotherapeutic drugs to provide synergistic effects in treating cancers such as melanoma.

[0381] FGFR kinase family: The FGFRs (i.e., Fibroblast Growth Factor Receptors) comprise a family of related but individually distinct tyrosine kinase receptors. They have a similar protein structure, with 3 immunoglobulin-like domains in the extracellular region, a single membrane spanning segment, and a cytoplasmic tyrosine kinase domain. The fibroblast growth factor receptors that have been identified are FGFRI, FGFR2, FGFR3, which is mutant in achondroplasia; and FGFR4. Sequence analysis of the 4.5-kb human FGFR2 gene shows an open reading frame encoding the typical membrane-spanning, tyrosine kinase receptor structure of the FGFR
gene family. A
discussion of FGFRI, FGFR2, FGFR3, and FGFR4 follows.

FGFRI
[0382] FGFRI: Target FGFRI (i.e., Fibroblast Growth Factor Receptor 1) is a 91.9 kDa transmembrane tyrosine kinase encoded by chromosome 8p11.2p11.1 (symbol:
FGFRI). FGFRI is also known as FMS-like tyrosine kinase 2 (i.e., Flt2). FGFRI is implicated in a variety of cancers (e.g. 8p11 syndrome, Braun & Shannon, Cancer Cell 2004, 5:203). Additionally, FGFR1 is an important mediator of tumor angiogenesis (Compagni et al., Cancer Res. 2000, 60:7163). The X-ray crystallographic structure of FGFR1 bound to fibroblast growth factor 2 has been reported by Plotnikov et al (Cell 1999, 98: 641-650). Rossi et al report an FGFR1 P252R
mutation in families affected by Pfeiffer syndrome (Rossi et al., Clin Dysmorphol. 2003, 12(4):269-74). FGFR1 inhibitors may be useful in treating 8p11 myeloproliferative syndrome.

[0383] FGFR2: Target FGFR2 (i.e., Fibroblast Growth Factor Receptor 2) is a 92 kDa transmembrane tyrosine kinase encoded by chromosome 10g26 (symbol: FGFR2).
According to OMIM, from a human tumor cDNA library, Houssaint et al. (Proc. Nat. Acad.
Sci.1990, 87: 8180-8184) isolated a gene encoding a putative receptor-like protein-tyrosine kinase that they called TK14.
The amino acid sequence was closely related to that of the mouse protein bek (bacterially expressed kinase), and more distantly related to the sequences of a chicken basic fibroblast growth factor receptor (73% sequence homology) and its presumed human equivalent, the FLG
protein.
Overexpression of the TK14 protein by transfection of COS-1 cells led to the appearance of new cell-surface binding sites for both acidic and basic fibroblast growth factors (OMIM MIM Number:
176943: 04/06/2006).

[0384] Sequence analysis of the 4.5-kbp human FGFR2 gene shows an open reading frame encoding the typical membrane-spanning, tyrosine kinase receptor structure of the FGFR gene family.
Two alternative gene products have been characterized: KGFR and BEK. These two isoforms are identical except for a 49-amino acid sequence spanning the second half of the third Ig loop in the extracellular region. This local diversity is due to the presence of alternative exons within FGFR2, exon B being expressed in the BEK product and exon K26 in KGFR. Control of these alternative splice sites is thought to involve transacting factors (Gilbert et al., Molec.
Cell. Biol. 1993, 13: 5461-5468). The variation in expressed gene product is highly significant because the ligand-binding characteristics of KGFR and BEK are quite distinct. Furthermore, they have different patterns of expression in murine embryogenesis. Whereas KGFR appears to have a role in skin development, BEK is preferentially expressed in osteogenesis. BEK transcripts are concentrated in the frontal bones, maxilla, mandibula, and ossicles of the middle ear.

[0385] To elucidate the structural determinants governing specificity in FGF
signaling, Plotnikov et al. (Cell 2000, 101: 413-424) determined the crystal structures of FGF1 and FGF2 complexed with the immunoglobulin-like ligand-binding domains 2 and 3 (D2 and D3) of FGFR1 and FGFR2, respectively. They found that highly conserved FGF-D2 and FGF-linker (between D2 and D3) interfaces define a general binding site for all FGF-FGFR complexes.
Specificity is achieved through interactions between the N-terminal and central regions of FGFs and 2 loop regions in D3 that are subject to alternative splicing. These structures provide a molecular basis for FGF1 as a universal FGFR ligand and for modulation of FGF-FGFR specificity through primary sequence variations and alternative splicing (OMIM MIM Number: 176943: 04/06/2006).

[0386] Defects in FGFR2 are a cause of Crouzon Syndrome (CS); also called craniofacial dysostosis type I (CFD1). CS is an autosomal dominant syndrome characterized by craniosynostosis (premature fusion of the skull sutures), hypertelorism, exophthalmos and external strabismus, parrot-beaked nose, short upper lip, hypoplastic maxilla, and a relative mandibular prognathism (OMIM MIM Number:
#123500: 05/04/2000).

[0387] Further, defects in FGFR2 are a cause of Jackson-Weiss syndrome (JWS).
JWS is an autosomal dominant craniosynostosis syndrome characterized by craniofacial abnormalities and abnormality of the feet including broad great toes with medial deviation and tarsal-metatarsal coalescence (OMIM MIM Number: 176943: 04/06/2006).

[0388] Further, defects in FGFR2 are a cause of Apert Syndrome, also known as acrocephalo-syndactyly type I (ACS1), which is characterized by craniosynostosis (premature fusion of the skull sutures) and severe syndactyly (cutaneous and bony fusion of the digits), and is autosomal dominant.
[0389] Further, defects in FGFR2 are a cause of Pfeiffer Syndrome (PS), also known as acrocephalosyndactyly type V (ACS5), which is characterized by craniosynostosis with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly. Three subtypes of PS have been described: mild autosomal dominant form (type 1); cloverleaf skull, elbow ankylosis, early death, sporadic (type 2);
craniosynostosis, early demise, sporadic (type 3).

[0390] Further, defects in FGFR2 are the cause of beare-stevenson cutis gyrata syndrome (BSCGS) which is an autosomal dominant condition characterized by the furrowed skin disorder of cutis gyrata, acanthosis nigricans, craniosynostosis, craniofacial dysmorphism, digital anomalies, umbilical and anogenital abnormalities and early death (OMIM MIM Number: 176943:
04/06/2006).

[0391] Further, defects in FGFR2 are the cause of Antley-Bixler syndrome which is characterized by trapezoidocephaly, midface hypoplasia, humeroradial synostosis, bowing of femora, fractures and other abnormalities (OMIM MIM Number: #207410: 11/29/2005). FGFR2 inhibitors may be useful in treating Crouzon Syndrome, Jackson-Weiss Syndrome, Apert Syndrome, craniosynostosis, Pfeiffer Syndrome, acrocephalo syndactyly type V, and Beare-Stevenson Cutis Gyrata Syndrome.

[0392] FGFR3: Target kinase FGFR3 (i.e., Fibroblast Growth Factor Receptor 3) is a 87.7 kDa transmembrane tyrosine kinase encoded by chromosome 4p16.3 (symbol: FGFR3). As a member of the fibroblast growth factor family, FGFR3 is involved in a variety of activities, including mitogenesis, angiogenesis, and wound healing. Furthermore, FGFR3 plays a role in the development and maintenance of bone and brain tissue. FGFR3 regulates bone growth by limiting the formation of bone from cartilage, particularly in the long bones. In addition, FGFR3 is activated by translocation in approximately 15% of multiple myeloma (Trudel, S., Blood 2004, 103:3521).
FGFR3 inhibitors may be useful in treating angiogenesis disorders, wounds, achondroplasia, Muenke craniosynostosis, Crouzon syndrome, acanthosis nigricans, thanatophoric dysplasia, bladder carcinomas, and multiple myeloma.

[0393] FGFR4: Target kinase FGFR4 (i.e., Fibroblast Growth Factor Receptor 4) is a 88.0 kDa transmembrane tyrosine kinase encoded by chromosome 5g35.3 (symbol: FGFR4).
According to OMIM, Partanen et al. (EMBO J. 1991, 10: 1347-1354) reported the cDNA cloning and analysis of a novel member of the fibroblast growth factor receptor (FGFR) gene family expressed in K562 erythroleukemia cells. Its deduced amino acid sequence was 55% identical with the previously characterized FGFRs, FLG (FGFRI) and BEK, and had the structural characteristics of an FGFR
family member including 3 immunoglobulin-like domains in its extracellular part. The expression pattern of FGFR4 was found to be distinct from that of FLG and BEK and also distinct from that of FGFR3, which had also cloned from K562 erythroleukemia cells, To elucidate further the physiologic relevance of protein-tyrosine kinases and to search for additional members of the gene family as possible factors in carcinogenesis, Holtrich et al. (Proc. Nat.
Acad. Sci. 1991, 88: 10411-10415) amplified mRNA from lung tissue by the polymerase chain reaction (PCR) using PTK-specific primers followed by sequencing of the clones. They identified a novel protein-tyrosine kinase, which they called TKF (tyrosine kinase related to fibroblast growth factor receptor). Among a wide variety of cells and tissues tested, including human lymphocytes and macrophages, TKF was found to be expressed only in lung and in some tumors of lung origin as well as in malignancies not derived from lung tissues. Sequence comparison has demonstrated that TKF is identical to FGFR4 (OMIM MIM Number: 134935: 05/03/2002).

[0394] The FGFR4 protein interacts with specific growth factors to conduct signals from the environment outside the cell to the nucleus. Animal studies indicate that the FGFR4 gene is involved in muscle development and the maturation of bone cells in the skull. FGFR4 may also play a role in the development and maintenance of specialized cells (called foveal cones) in the light-sensitive layer (retina) at the back of the eye. Aberrant expression of FGFR4 is correlated with cancer of the breast, ovary, endometrium, and fallopian tube, and with leiomyosarcoma. FGFR4 inhibitors may be useful in treating cancer of the breast, lung, colon, medullary thyroid, pancreas, ovary, prostate, endometrium, and fallopian tube, head and neck squamous cell carcinomas and leiomyosarcoma.
[0395] FI11: Target kinase Fltl (i.e., Fms like tyrosine kinase 1) is a 150.7 kDa transmembrane tyrosine kinase encoded by chromosome 13g12 (symbol: FLT1), also known as VEGFR1 (i.e., Vascular Endothelial Growth Factor Receptor 1). According to OMIM, oncogene FLT belongs to the SRC gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation via interaction with PLC-gammas, PTPN1 1, GRB2, CRK, NCK1 and other proteins.
The name is due to the resemblance of the sequence structure of the FLT gene to that of the FMS gene. VEGF and its high-affinity binding receptors, the tyrosine kinases Flkl and Fltl, are thought to be important for the development of embryonic vasculature. Studying transgenic mice in whom the Flkl gene was disrupted, Shalaby et al. (Nature 1995, 376: 62-65) demonstrated a total failure of embryonic mice to develop blood vessels and failure of blood island formation in the yolk sac.
Fong et al. (Nature 1995, 376: 65-69) reported that in mice Fltl is essential for the organization of embryonic vasculature, but is not essential for endothelial cell differentiation. Transgenic mouse embryos homozygous for a targeted mutation in the Fltl locus formed endothelial cells in both embryonic and extraembryonic regions, but assembled these cells into abnormal vascular channels and died in utero at mid-somite stages. At earlier stages, the blood islands of homozygous mice were abnormal, with angioblasts in the interior as well as on the periphery. Fong et al. (ibid.) suggested that the Fltl signaling pathway may regulate normal endothelial cell-cell or cell-matrix interactions during vascular development (OMIM MIM Number: 165070: 03/27/2006). Flt 1 inhibitors may be useful in treating non-small cell lung carcinoma, prostate carcinoma, and colorectal cancer.

[0396] FIt3: Target kinase Flt3 (i.e., Fms-like tyrosine kinase 3) is a transmembrane tyrosine kinase of 112.8 kDa encoded by chromosome 13g12 (symbol: FLT3). According to OMIM, Rosnet et al.
(Genomics 1991, 9: 380-385) isolated a novel member of the class 3 receptors discussed above. They demonstrated that this gene of the tyrosine kinase family, called FLT3, has strong sequence similarities with other members of the group. Lymphohematopoietic stem cells serve as a reservoir for virtually all blood cells but make up only approximately 0.01% of human or murine marrow cells.
The ability to isolate and expand this population has clinical applications in bone marrow transplantations for cancer and genetic diseases. Small et al. (Proc. Nat.
Acad. Sci. 1994, 91: 459-463) cloned the cDNA for stem cell tyrosine kinase 1, the human homolog of murine F1k2/Flt3, from a CD34+ hematopoietic stem cell-enriched library. The cDNA encoded a protein of 993 amino acids with 85% identity and 92% similarity to the murine homolog. STK1, which is identical to FLT3, is a member of the type III receptor tyrosine kinase family that includes KIT, FMS, and platelet-derived growth factor receptor. STK1 expression in human blood and marrow is restricted to CD34+ cells, a population greatly enriched by stem/progenitor cells. Antisense oligonucleotides directed against STK1 sequences inhibited hematopoietic colony formation, most strongly in long-term bone marrow cultures. The data suggested that STK1 may function as a growth factor receptor on hematopoietic stem and/or progenitor cells (OMIM MIM Number: 136351: 03/03/2005).

[0397] Levis et al., state that Internal tandem duplication (ITD) mutations of the receptor tyrosine kinase FLT3 have been found in 20% to 30% of patients with acute myeloid leukemia (AML). These mutations constitutively activate the receptor and appear to be associated with a poor prognosis. In their study, dose-response cytotoxic assays were performed with AG 1295, a tyrosine kinase inhibitor active against FLT3, on primary blasts from patients with AML, and they found that AG1295 was specifically cytotoxic to AML blasts harboring FLT3/ITD mutations. They suggest that these mutations contribute to the leukemic process and that the FLT3 receptor represents a therapeutic target in AML (Levis et al., Blood 2001, 98:885-887). F1t3 inhibitors may be useful in treating acute myeloid leukemia, myelodysplastic syndrome, acute lymphoblastic leukemia.

[0398] FIt4: Target kinase F1t4 (i.e., Fms-like tyrosine kinase 4) is a transmembrane tyrosine kinase of 145.6 kDa encoded by chromosome 5q35.3 (symbol: FLT4). F1t4 is also known as VEGFR3 (i.e., Vascular Endothelial Growth Factor Receptor 3). According to OMIM, by screening a placenta cDNA library with a mouse Flt3 probe, Galland et al. (Genomics 1992, 13: 475-478) isolated a human gene encoding a putative receptor-type tyrosine kinase, FLT4. The deduced amino acid sequence of the intracellular portion of the molecule showed that it was strongly related to FLT1 and KDR and to a lesser degree to members of the class 3 receptor-type tyrosine kinases: FMS, PDGFR, KIT, and FLT3. Primary lymphoedema, a rare, autosomal dominant disorder that leads to a disabling and disfiguring swelling of the extremities and, when untreated, tends to worsen with time, has been linked to the FLT4 locus (Karkkainen et al., Nat. Genet. 2000, 25: 153-9). All disease-associated alleles analyzed had missense mutations and encoded proteins with an inactive tyrosine kinase, preventing downstream gene activation (OMIM MIM Number: 136352: 11/19/2003).
F1t4 inhibitors may be useful in treating primary lymphoedema.

[0399] Fms: Target kinase Fms (i.e., feline McDonough sarcoma) is a member of the family of genes originally isolated from the Susan McDonough strain of feline sarcoma viruses. Fms is a transmembrane tyrosine kinase of 108.0 kDa coded by chromosome 5q33.2-q33.3 (symbol: CSF1R).

The structure of the transmembrane receptor Fms comprises two Ig-like domains, a IgC2-like domain, two additional Ig-like domains, a TM domain, and the TK domain.

[0400] Fms is the receptor for the macrophage colony-stimulating factor (M-CSF), and is crucial for the growth and differentiation of the monocyte-macrophage lineage. Upon binding of M-CSF to the extracellular domain of Fms, the receptor dimerizes and trans-autophosphorylates cytoplasmic tyrosine residues.

[0401] M-CSF, first described by Robinson and co-workers (Blood. 1969, 33:396-9), is a cytokine that controls the production, differentiation, and function of macrophages. M-CSF stimulates differentiation of progenitor cells to mature monocytes, and prolongs the survival of monocytes.
Furthermore, M-CSF enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis, and secondary cytokine production of additional factors in monocytes and macrophages. Examples of such additional factors include granulocyte colony stimulating factor (G-CSF), interleukin-6 (IL-6), and interleukin-8 (IL-8). M-CSF stimulates hematopoiesis, promotes differentiation and proliferation of osteoclast progenitor cells, and has profound effects on lipid metabolism.
Furthermore, M-CSF is important in pregnancy. Physiologically, large amounts of M-CSF are produced in the placenta, and M-CSF is believed to play an essential role in trophoblast differentiation (Motoyoshi, Int J Hematol.
1998, 67:109-22). The elevated serum M-CSF levels of early pregnancy may participate in the immunologic mechanisms responsible for the maintenance of the pregnancy (Flanagan & Lader, Curr Opin Hematol. 1998, 5:181-5).

[0402] Aberrant expression and/or activation of Fins has been implicated in acute myeloid leukemia, AML (Ridge et al, Proc. Nat. Acad. Sci., 1990, 87:1377-1380).
Mutations at codon 301 are believed to lead to neoplastic transformation by ligand independence and constitutive tyrosine kinase activity of the receptor. The tyrosine residue at codon 969 has been shown to be involved in a negative regulatory activity, which is disrupted by amino acid substitutions.
Accordingly, Fins mutations are most prevalent (20%) in chronic myelomonocytic leukemia and AML
type M4 (23%), both of which are characterized by monocytic differentiation.

[0403] A condition related to AML is chronic myeloid leukemia (CML). During the myeloid blast crisis (BC) of CML, non-random additional chromosome abnormalities occur in over 80% of patients.
However, these cytogenetic changes have been reported to precede the clinical signs of CML-BC by several months to years suggesting that other biological events may participate in the multistep process of acute transformation of CML. The autocrine production of growth factors has been shown to occur in several hematological malignancies and particularly in AML.
Specchia et al [Br J
Haematol. 1992 Mar; 80(3):310-6] have demonstrated that IL-1 beta gene is expressed in almost all cases of CML in myeloid blast crisis, and that a high proportion of cases showed constitutive expression of the M-CSF gene. Many of the same patients in the Specchia et al study demonstrated simultaneous co-expression of Fms. After exposure of leukemic cells to phorbol myristate acetate (PMA), release of M-CSF protein was documented in three of five patients studied; however, no significant interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF) or granulocyte colony-stimulating factor (G-CSF), was detected in these patients.
This demonstrates that different patterns of growth factors secretion exist in AML and CML, and that distinct molecular events are likely involved in the control of leukemic proliferation.

[0404] The observation that production of M-CSF, the major macrophage growth factor, is increased in tissues during inflammation (Le Meur et al, J. Leukocyte Biology.
2002;72:530-537) provides a role for Fms in certain diseases. For example, COPD is characterized by airflow limitation that is not fully reversible. The airflow limitation is usually progressive and associated with an abnormal inflammatory response of the lungs to noxious particles or gases. The chronic inflammation of COPD is observed through the airways, parenchyma, and pulmonary vasculature. The inflammatory cell population consists of neutrophils, macrophages, and T
lymphocytes, along with eosinophils in some patients. Macrophages are postulated to play an orchestrating role in COPD
inflammation by releasing mediators such as TNF-a, IL-8 and LTB4, which are capable of damaging lung structures and/or sustaining neutrophilic inflammation, [0405] Further, M-CSF/fins signaling is critical to osteoclast formation and survival of osteoclast precursors. For example, estrogen loss in menopause results in increased M-CSF
and thus increased osteoclast number and bone resorption which leads to increased risk of fracture and osteoporosis.
Accordingly, blockage of this signal is a target for the inhibition of bone resorption (Teitelbaum, Science. 2000;289:1504; Rohan, Science. 2000;289:1508).

[0406] Atherosclerosis, an inflammatory disease of the vessel walls, is associated with significant morbidity and mortality. A effect for Fms inhibition in the treatment and prevention of atherosclerosis depends on several observations (Libby, Nature. 2002;420:868-874). First, monocytes resident in the arterial intima increase expression of scavenger receptors and internalize modified lipoproteins. The resulting lipid-laden macrophages develop into foam cells characteristic of the atherosclerotic lesion. Macrophages in atheroma secrete cytokines and growth factors involved in lesion progression. Additionally, macrophages replicate within the intima.
Through Fms, M-CSF
activates the transition from monocyte to lipid-laden macrophage and augments expression of scavenger receptor A. Indeed, atherosclerotic plaques over-express M-CSF which is critical for atherosclerotic progression. Mice deficient in M-CSF have been found to experience less severe atherosclerosis than mice with normal M-CSF (Rajavashisth, et. al., J. Clin.
Invest. 1998;101:2702-2710; Qiao, et. al., Am. J. Path. 1997;150:1687-1699). Accordingly, inhibitors of Fms disrupt M-CSF

signaling, compromising monocyte to macrophage foam cell progression, macrophage survival and replication, and cytokine signaling that participates in lesion progression.

[0407] Wegener's granulomatosis, also known as vasculitis, is characterized by granulomatous inflammation of the blood vessels with necrosis. This inflammation limits blood flow to organs with consequent damage. Although the disease can involve any organ system, Wegener's granulomatosis mainly affects the respiratory tract (i.e., sinuses, nose, trachea, and lungs) and the kidneys. The endothelium plays a central role in the immunopathology of several vascular disorders in many inflammatory conditions such as Wegener's granulomatosis in which use of intravenous immunoglobulin (IV Ig) has been shown to be beneficial (see e.g., Basta et al, J Clin Invest 1994, 94:1729-1735). It has been reported (Xu et al, Am. J. Path., 1998;153:1257-1266) that IV Ig inhibits endothelial cell proliferation in a dose- and time-dependent manner and down-regulates the expression of adhesion molecule mRNA (ICAM-1 and VCAM-1), chemokine mRNA (MCP-1, M-CSF, and GM-CSF), and proinflammatory cytokine mRNA (TNF-ce, IL-113, and IL-6) induced by TNF-cr or IL-113. These results may explain, at least in part, the therapeutic effect of IV Ig in vascular and inflammatory disorders. Additionally, these results suggest that inhibition of M-CSF activity at the level of interaction with Fms is an efficacious treatment strategy.

[0408] The role of M-CSF and Fms in emphysema appears to involve the regulation of elastin metabolism through control of matrix metalloproteins. M-CSF has a role in the modulation of the accumulation and function of alveolar macrophages (AMs) in vivo (Shibata et al, Blood 2001, 98: pp.
2845-2852). Osteopetrotic (Op/Op) mice have no detectable M-CSF and show variable tissue-specific reductions in macrophage numbers. Accordingly, it was hypothesized that AMs would be decreased in number and have altered function in Op/Op mice because of the absence of M-CSF.
Shibata et al found that lung macrophages identified in lung sections were decreased in number in 20-day-old Op/Op mice but not Op/Op mice older than 4 months compared with findings in age-matched littermate controls. The numbers of AMs recovered by bronchoalveolar lavage (BAL) were also reduced in young but not adult Op/Op mice compared with controls. Importantly, AMs of Op/Op mice spontaneously release higher levels of matrix metalloproteinases (MMPs) than AMs of controls.
Consistent with an increased release of MMP, Op/Op mice have abnormal elastin deposition and spontaneously develop emphysema in the absence of molecular or cellular evidence of lung inflammation. Accordingly, the modulation of metalloelastase activity in macrophages by M-CSF
may control the degradation of elastin fibers in lungs or blood vessels.

[0409] Metastatic cancer cells cause bone destruction, with associated fracture, pain, deformation, and hypercalcaemia, due to production of osteoclasticogenic factors including M-CSF by tumor cells (Clohisy et al, Clin. Orthop. 2000, 373: 104-14). Binding of M-CSF to the Fms product stimulates formation of osteoclasts and osteolytic activity (Kodama et al, J. Exp. Med.
1991, 173: 269-72; Feng et a1, Endocrinology 2002, 143: 4868-74). Accordingly, inhibition of osteoclast activity at the level of Fms offers a compelling target for amelioration of bone metastasis.

[0410] Nephritis is inflammation of the kidneys. It may be caused for example by a bacterial infection of the kidneys or exposure to a toxin. However, nephritis more commonly develops from an abnormal immune reaction, which can occur, for example, when an antibody attacks either the kidney itself or an antigen attached to kidney cells, or when an antigen-antibody complex formed elsewhere in the body attachs to cells in the kidney. Some types of nephritis involve infiltration of kidney tissues by white blood cells and deposits of antibodies. In other types of nephritis, inflammation may consist of tissue swelling or scarring without white blood cells or antibodies. Furthermore, nephritis can occur anywhere in the kidneys. With respect to the glomeruli, progressive damage to glomeruli causes urine production to fall and metabolic waste products to build up in the blood. When damage to glomeruli is severe, inflammatory cells and injured glomerular cells accumulate, compressing the capillaries within the glomerulus and interfering with filtration. Scarring may develop, impairing kidney function and reducing urine production. In some cases, microthrombi may form in the small blood vessels, further decreasing kidney function. Less commonly, nephritis involves the tubulointerstitial tissues; such inflammation is called tubulointerstitial nephritis. When inflammation damages the tubules and the tubulointerstitial tissues, the kidneys may become unable to concentrate urine, eliminate (excrete) metabolic waste products from the body, or balance the excretion of sodium and other electrolytes, such as potassium. When the tubules and tubulointerstitial tissues are damaged, kidney failure often develops. Accordingly, inhibition of Fms offers a target for therapeutic intervention in nephritis due to the modulation of the inflammatory response comprising the etiology of the disease.

[0411] Lupus nephritis, i.e., renal involvement in systemic lupus erythematosus (SLE), is a common disease manifestation with a poor prognosis. At least three potentially overlapping, inununo-pathogenic mechanisms for lupus nephritis are supported by experimental data.
First, circulating immune complexes consisting chiefly of DNA and anti-DNA are deposited in the kidney. Resulting complement activation and chemotaxis of neutrophils leads to a local inflammatory process. Second, in situ formation of antigen and antibody complexes may similarly lead to complement activation and leucocyte mediated injury. Third, antibodies against specific cellular targets may produce renal injury. An additional mechanism is observed in SLE patients with the antiphospholipid antibody syndrome. Glomerular thrombosis can result from the hypercoagulability that accompanies antibodies directed against negatively charged phospholipid-protein complexes (e.g. biologic false positive VDRL, anticardiolipin antibodies, and lupus anticoagulant). Mesangial lupus nephritis is accompanied by normal diagnostic findings or with a mild degree of proteinuria but typically absence of hypertension or abnormal urinary sediment. Focal and diffuse proliferative lupus glomerulonephritis are often associated with the worst prognosis for renal survival and can be accompanied by nephrotic syndrome, significant hypertension and abnormal urine sediment.
Membranous lupus nephritis often presents with proteinuria, moderate to high grade, but usually normal urinary sediment in the absence of hypertension. Mesangial lupus nephropathy is generally associated with an excellent prognosis, whereas proliferative lupus nephropathy, especially diffuse variant, is often characterized by hypertension, red cell casts and significant deterioration of renal function. Nephrotic syndrome in the absence of hypertension, active urinary sediment or significant hypocomplementemia suggest the membranous variant of lupus nephropathy.
Membranous nephropathy generally is associated with a good prognosis and relative preservation of renal function.
However, in the presence of persistent nephrotic range proteinuria, membranous lupus nephropathy can, in fact, lead to loss of renal function and end stage renal disease (ESRD). Accordingly, inhibition of Fms offers a target for therapeutic intervention in lupus due to the modulation of the inflammatory response comprising the etiology of the disease.

[0412] Macrophage accumulation is a prominent feature in many forms of glomerulonephritis.
Local proliferation of macrophages within the kidney has been described in human and experimental glomerulonephritis and may have an important role in augmenting the inflammatory response. Isbel et al (Nephrol Dial Transplant 2001, 16: 1638-1647) examined the relationship between local macrophage proliferation and renal expression of M-CSF. Glomerular and tubulointerstitial M-CSF
expression was found to be up-regulated in human glomerulonephritis, being most prominent in proliferative forms of disease. Because this correlates with local macrophage proliferation, it suggests that increased renal M-CSF production plays an important role in regulating local macrophage proliferation in human glomerulonephritis. In a model of renal inflammation (UUO- unilateral ureteric obstruction) anti-Fms antibody treatment reduced macrophage accumulation (Le Meur et.al., J
Leukocyte Biology, 2002, 72: 530-537). Accordingly, inhibition of Fms offers a target for therapeutic intervention in glomerulonephritis.

[0413] Insulin resistance and obesity are hallmark of type II diabetes and there is a strong correlation exists between insulin resistance and abdominal visceral fact accumulation (Bjorntrop, Diabetes Metab. Res. Rev., 1999, 15: 427-441). Current evidence indicates that macrophages accumulating in adipose tissue release TNF-a and other factors that cause adipocyte changes (hypertrophy, lipolysis, reduced insulin sensitivity) and also promote insulin resistance in surrounding tissues. Therefore, macrophage accumulation in type 2 diabetes is important for disease progression.
Accordingly, inhibition of Fms has potential in preventing the development of insulin resistance and hyperglycemia.

[0414] Similarly, the observation that production of M-CSF, the major macrophage growth factor, is increased in tissues during inflammation points out a role for Fms in diseases, such as for example inflammatory diseases. More particularly, because elevated levels of M-CSF are found in the disease state, modulation of the activity of Fms can ameliorate disease associated with increased levels of M-CSF.

[0415] Fms inhibitors may be useful in treating to immune disorders, including rheumatoid arthritis, systemic lupus erythematosis (SLE), Wegener's granulomatosis, and transplant rejection, inflammatory diseases including Chronic Obstructive Pulmonary Disease (COPD), emphysema, and atherosclerosis, metabolic disorders, including insulin resistance, hyperglycemia, and lipolysis, disorders of bone structure or mineralization, including osteoporosis, increased risk of fracture, hypercalcemia, and bone metastases, kidney diseases, including nephritis (e.g.
glomerulonephritis, interstitial nephritis, Lupus nephritis), tubular necrosis, diabetes-associated renal complications, and hypertrophy and cancers, including multiple myeloma, acute myeloid leukemia, chronic myeloid leukemia (CML), breast cancer, and ovarian cancer.

[0416] Frk: Target kinase Frk (Fyn-related kinase) is a 58.5 kDa tyrosine kinase encoded by chromosome 6g21-g22.3 (symbol: FRK). The structure comprises an SH2, an SH3 and a tyrosine kinase domain. Hosoya et al., report the the identification of a SRC-like tyrosine kinase gene, FRK
(Fyn-related kinase), fused with ETV6 in a patient with acute myelogenous leukemia carrying t(6;12)(g2l;p 13). The ETV6/FRK protein was shown to be constitutively autophosphorylated on its tyrosine residues. ETV6/FRK phosphorylated histones H2B and H4 in vitro to a greater extent than did FRK, suggesting it had elevated kinase activity. ETV6/FRK could transform both Ba/F3 cells and NIH3T3 cells, which depended on its kinase activity (Hosoya et al., Genes Chromosomes Cancer 2005, 42(3):269-79). Welsh et al. concluded that FRK/RAK contributes to cytokine-induced beta-cell death, and inhibition of this kinase could provide means to suppress beta-cell destruction in Type I
diabetes (Welsh et al., Biochem J 2004, 382(l):261-8). Frk inhibitors may be useful in treating acute myeloid leukemia and type I diabetes.

[0417] Fyn: Target kinase Fyn (i.e., Fyn oncogene related to Src, Fgr, Yes) is a 60.6 kDa non-receptor tyrosine kinase encoded by chromosome 6g21 (symbol: FYN). Fyn is involved in regulation of mast cell degranulation in a synergistic confluence of Fyn and Lyn (i.e., v-Yes-1 Yamaguchi sarcoma viral related oncogene homolog) pathways at the level of protein kinase C and calcium regulation. Fyn inhibitors may be useful in treating Alzheimer's disease, schizophrenia and in prevention of metastases, e.g. in melanoma and squamous cell carcinoma.

[0418] Gsk3o, Gsk3(3: Target kinase Gsk3j (i.e., Glycogen synthase kinase 3 beta) is a 46.8 kDa STK encoded by chromosome 3g13.3 (symbol: GSK3B). Target kinase Gsk3a (i.e., Glycogen synthase kinase 3 alpha) is a 51.0 kDa STK encoded by chromosome 19g13.2 (symbol:
GSK3A).Gsk3 is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha and beta, show a high degree of amino acid homology (Stambolic & Woodgett Biochein. J. 1994, 303:
701-704). GSK3B
is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., Biochim. Biophys. Acta 1992, 1114: 147-162). The X-ray crystallographic structure of Gsk3 has been reported by Dajani et al. (Cell 2001, 105: 721-732). Klein & Melton (Proc. Nat. Acad. Sci.
1996, 93: 8455-8459) proposed that Gsk3 is the endogenous target of lithium in diverse systems. For example, lithium potently and specifically inhibits Gsk3 activity in vitro.
This suggests a mechanism whereby lithium can mimic insulin action, and lithium inhibition of the Gsk3 pathway in the brain could explain the actions of lithium action in manic-depressive illness in addition to its effects on development and its insulinlike activity.

[04191 Phiel et al., show that therapeutic concentrations of lithium, a GSK-3 inhibitor, block the production of Abeta peptides by interfering with APP cleavage at the gamma-secretase step, but do not inhibit Notch processing. Importantly, lithium also blocks the accumulation of Abeta peptides in the brains of mice that overproduce APP. The target of lithium in this setting is GSK-3alpha, which is required for maximal processing of APP. Since GSK-3 also phosphorylates tau protein, the principal component of neurofibrillary tangles, inhibition of GSK-3alpha offers a new approach to reduce the formation of both amyloid plaques and neurofibrillary tangles, two pathological hallmarks of Alzheimer's disease (Phiel et al., Nature 2003, 423:435-439). Eldar-Finkelman states that GSK-3 inhibitors might prove useful as therapeutic compounds in the treatment of conditions associated with elevated levels of enzyme activity, such as type 2 diabetes and Alzheimer's disease. The pro-apoptotic feature of GSK-3 activity suggests a potential role for its inhibitors in protection against neuronal cell death, and in the treatment of traumatic head injury and stroke. Finally, selective inhibitors of GSK-3 could mimic the action of mood stabilizers such as lithium and valproic acid and be used in the treatment of bipolar mood disorders (Eldar-Finkelman, Trends Mol Med 2002, 8:126-132). Martinez et al. state that glycogen synthase kinase 3 (GSK-3) was initially described as a key enzyme involved in glycogen metabolism, but is now known to regulate a diverse array of cell functions. Two forms of the enzyme, GSK-3alpha and GSK-3beta, have been previously identified. Small molecules inhibitors of GSK-3 may, therefore, have several therapeutic uses, including the treatment of neurodegenerative diseases, diabetes type II, bipolar disorders, stroke, cancer, and chronic inflammatory disease (Martinez et al., Med Res Rev 2002, 22(4):373-84). GSK
inhibitors may be useful in treating CNS disorders such as Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, diabetes type II, bipolar disorders, stroke, cancer, chronic inflammatory disease, leucopenia, schizophrenia, chronic pain, neuropathic pain, and traumatic head injury.

[04201 HCK: Target kinase HCK (hemopoietic cell kinase) is a 59.5 kDa tyrosine kinase encoded by chromosome 20gl 1.21 (symbol: HCK). The protein structure comprises an SH3, and SH2 and a bipartite kinase domain. HCK inhibitors may be useful in treating chronic myelogenous leukemia and acute lymphocytic leukemia.

[0421] Her2/Erbb2: Target kinase Her2/Erbb2 (i.e., Human EGF receptor 2) is a 137.9 kDa transmembrane tyrosine kinase encoded by chromosome 17g11.2-g12/17g21.2 (symbol: ERBB2).
According to OMIM, the ERBB2 locus 17g21 is the chromosome 17 breakpoint in acute promyelocytic leukemia (APL). Amplification of ERBB2 is observed in human salivary gland adenocarcinoma (Semba et al., Proc. Nat. Acad. Sci. 1985, 82:6497-6501) and in a gastric cancer cell line (Fukushige et al., Molec. Cell. Biol. 1986, 6:955-958). Overexpression of ERBB2 has been implicated in the neoplastic transformation of prostate cancer. Interleukin-6 (IL6) is a cytokine that was initially recognized as a regulator of immune and inflammatory responses, but also regulates the growth of many tumor cells, including prostate cancer. ui et al. showed that treatment of a prostate cancer cell line with 1L6 induces tyrosine phosphorylation of ERBB2 and ERBB3, but not ERBB1/EGFR. ERBB2 also forms a complex with the gpl30 subunit of the 1L6 receptor (IL6R) in an 1L6-dependent manner. This association is important because the inhibition of ERBB2 activity results in abrogation of 1L6-induced MAPK activation (Qui et al., Nature 1998, 393:83-85). Thus, ERBB2 is a critical component of 1L6 signaling through the MAP kinase pathway.
Additionally, overexpression of ERBB2 confers Taxol resistance in breast cancers by inhibiting p34 (CDC2) activation (Yu et al., Molec. Cell 1998, 2:581-91) (OMIM MIM Number: 164870:
01/30/2006).
Her2/Erbb2 inhibitors may be useful in treating prostate and breast cancer.

[0422] Her4/Erbb4: Target kinase Her4/Erbb4 (i.e., Human EGF receptor 4) is a 146.8 kDa transmembrane tyrosine kinase encoded by chromosome 2q33.3-q34 (symbol:
ERBB4). According to OMIM, the HER4/ERBB4 gene is a member of the type I receptor tyrosine kinase subfamily that includes EGFR, ERBB2, and ERBB3. The gene product of ERBB4 is a receptor for NDF/heregulin, which are essential for neuronal development. Her4/Erbb4 -/- mouse embryos exhibit axonal misprojections which correlate with aberrant migration of a subpopulation of hindbrain-derived cranial neural crest cells. Accordingly, Her4/Erbb4 signaling provides patterning information essential for the proper migration of neural crest cells (OMIM MIM Number:
600543: 07/27/2005).
Her4/Erbb4 inhibitors may be useful in treating childhood medulloblastoma.

[0423] IGF1R: Target kinase IGFIR (insulin-like growth factor 1 receptor) is a 154.8 kDa receptor tyrosine kinase encoded by chromosome 15g26.1 (symbol: IGF1R). Overexpressed in breast and prostate cancer, acting to enhance tumor cell survival. IGFIR inhibitors may be useful in treating prostate cancer and hepatocellular carcinoma.

[0424] IKK beta: Target kinase IKK beta (i.e., inhibitor of nuclear factor kappa B kinase beta) is a 86.6 kDa STK encoded by chromosome 8pl 1.2 (symbol: IKBKB). According to OMIM, IKK beta phosphorylates serine residues of I-kappa-B proteins which marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex.
Activated NF-KB
complex translocates into the nucleus and binds DNA at kappa-B-binding motifs.
Yin et al (Nature 396: 77-80, 1998) have shown that the antiinflammatory properties of aspirin and salicylate are mediated in part by their specific inhibition of 1KK-beta, thereby preventing activation by NF-kappa-B of genes involved in the pathogenesis of the inflammatory response. Rossi et al (403: 103-108, 2000) demonstrated a novel mechanism of antiinflammatory activity that was based on the direct inhibition and modification of the IKK-beta subunit of IKK. Since IKK-beta is responsible for the activation of NF-kappa-B by proinflammatory stimuli, Rossi et al. (ibid.) suggested that their findings explained how cyclopentenone prostaglandins function and can be used to improve the utility of COX2 inhibitors (OMIM MIM Number: 603258: 11/16/2005).

[0425] IKKbeta inhibitors may be useful in treating leukemia of T-cells, necrosis, neoplasms, insulin resistance, and malignant neoplasms.

[0426] Irak4: Target kinase Irak4 (i.e., Interleukin 1 receptor associated kinase 4) is a 51.5 kDa serine/threonine kinase encoded by chromosome 12g12 (symbol: IRAK4).
Interleukin-1 receptor associated kinases (e.g., IRAK1) are important mediators in the signal transduction of Toll-like receptor (TLR, e.g., TLR4) and IL1R family members are collectively referred to as TIRs. Irak4 functions in this signal transduction pathway. The structure of Irak4 comprises a DEATH domain adjacent a STK domain. The DEATH domain is a protein-protein interaction motif found in certain proteins of the apoptotic pathway.

[0427] Irak4 was originally identified as NY-REN-64, one of 65 human tumor antigens recognized by autologous antibodies from patients with renal cell carcinoma using serological analysis of recombinant cDNA expression libraries (SEREX). Sequence analysis of the NY-REN-64 cDNA
clone identified in the SEREX screen revealed a novel gene encoding a transcript of 2.8 kilobases and a predicted protein of 460 amino acids (Genbank Accession AF155118) noted to bear a protein kinase motif (Scanlan et al., Int. J. Cancer, 1999, 83, 456-464). Based on its homology to the other IL-1 receptor-associated kinases, this gene has more recently been placed in the IRAK family and given the name IL-1 receptor-associated kinase-4.

[0428] Irak4 is required for the efficient recruitment of 1RAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Irak4 Phosphorylates Irakl. Effective Irak4 functioning is crucial for protective immunity against specific bacteria, including pyogenic bacterial, but is redundant against other microorganism.

[0429] Irak4 inhibitors may be useful in treating immunodeficiency syndrome, Crohn's disease, ulcerative colitis, asthma, chronic bronchitis, cardio hypertrophy, and kidney hypertension.

[0430] j. Target kinase Itk (i.e., IL-2 inducible T-cell kinase) is a tyrosine kinase of 71.8 kDa encoded by chromosome 5g31-q32 (symbol: ITK). Itk is a T-cell specific homology of kinase Btk.
The EMT Tee family kinases are non-receptor type protein-tyrosine kinase that are highly expressed in many hematopoietic cell lines. The TEC-family protein tyrosine kinases ITK, RLK(TXK) and TEC have been identified as key components of T-cell-receptor signalling that contribute to the regulation of phospholipase C-gamma, the mobilization of Ca2+ and the activation of mitogen-activated protein kinases. Recent data also show that TEC kinases contribute to T-cell-receptor-driven actin reorganization and cell polarization, which are required for productive T-cell activation.
Functional studies have implicated TEC kinases as important mediators of pathways that control the differentiation of CD4+ T helper cells (Schwartzberg et al., 2005, Nature immunology, 5:284).

[0431] T cells express three TEC kinases, ITK, RLK and TEC, all of which are activated downstream of the T-cell receptor (TCR) (Berg, L. J et al., Annu. Rev.
Immunol., 2005, 23:549) and have been shown to be involved in signaling through the TCR (Schaeffer, E. M.
et al., 1999, Science, 284,:638). Although ITK, RLK and TEC are all found in T cells, they are expressed at different levels and by different subpopulations. (Lucas, J. A et al., 2003, Immunol.
Rev., 191;119. Colgan, J.
et al. 2004, Immunity, 21:189). High expression of TEC was seen in each of 3 patients examined with myelodysplastic syndrome(Sato K et al., 1994, Leukemia, 8:1663). Although no human disease has been associated with mutations of the TEC kinases that are expressed by T
cells, ITK-deficient mice have specific defects in T Helper 2 (TH2)-cell responses and reduced pathology in models of allergic asthma (Fowell et al. 1999, Immunity, 11:399). Specific ITK inhibitors reduce disease in a mouse model of allergic asthmal9, TEC kinases are activated through phosphorylation by SRC-family kinases, such as LCK, and recruitment to the plasma membrane through binding of Ptdlns(3,4,5)P3, where they are brought into TCRsignalling complexes through interactions with SLP76, LAT and other molecules (Bunnell, S. C. et al. 2000, J. Biol. Chem., 275:2219.).

[0432] Consistent with a role for ITK in allergic responses, increased ITK
expression has been seen in peripheralblood T cells from humans with atopic dermatitis(Matsumoto, Y. et al., 2002, hit. Arch.
Allergy Immunol. 129:327). Importantly, Itk -/- mice cannot mount effective TH2-cell responses to infection with many pathogens that are used to evaluate TH2-cell differentiation, including Nippostrongylus brasiliensis, Schistosoma mansoni and Leishmania major (Fowell, D. J. et al. 1999, Immunity, 11:399. Schaeffer, E. et al. 2001, Nature Immunol., 2:1183).

[0433] TH2-cell responses have been implicated in the pathology of allergic asthma, which is characterized by an increased number of TH2 cells in the lungs, increased TH2-cytokine production, increased mucus production in the lungs and inflammation of the airways (Cohn, L et al. 2004, Annu.
Rev.Immunol., 22:789). For several reasons, ITK, however, might be an ideal therapeutic target for TH2-cell-mediated diseases, provided that the inhibitor has a high degree of specificity. Itk inhibitors may be useful in treating allergic asthma.

[04341 Jakl: Target kinase Jakl (i.e., Janus kinase 1) is a 132 kDa tyrosine kinase encoded by chromosome lp3l.3 (symbol: JAK1). Jakl inhibitors may be useful in treating Hepatitis C virus infection.

[04351 Jak2: Target kinase Jak2 (i.e., Janus kinase 2) is a 130.7 kDa tyrosine kinase encoded by chromosome 9p24 (symbol: JAK2). Jak2 inhibitors may be useful in treating myeloproliferative disorders such as polycythaemia vera, myelofibrosis, essential thrombocythemia, myeloid metaplasia and leukemias, including acute lymphoblastic leukemia, chronic neutrophilic leukemia, juvenile myelomonocytic leukemia, CMML, Philadelphia chromosome-negative CML, megakaryocytic leukemia, and acute erythroid leukemia [04361 Jak3: Target kinase Jak3 (i.e., Janus kinase 3) is a 125.1 kDa tyrosine kinase encoded by chromosome 19p13.1 (symbol: JAK3). According to OMIM, JAK3 is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. Interleukin-2 (IL2) signaling requires the dimerization of IL2 receptor-beta (IL2RB) with the common gamma chain (gamma-c; 1L2RG). Mutations in the IL2RG gene cause X-linked severe combined immunodeficiency. Interleukins 1L2, 1L4, IL7,1L9, and El, 15, whose receptors are known to contain the common gamma chain, induce the tyrosine phosphorylation and activation of Jak3. Truncations of gamma-c and a point mutation of gamma-c, causing moderate X-linked combined immunodeficiency, decrease the association between the common gamma chain and Jak3.
Since mutations in the IL2RG gene in at least some XSCID and XCID patients prevent normal Jak3 activation, mutations in Jak3 may result in an XSCID-like phenotype (OMIM MIM
Number: 600173:
04/04/2006). A related kinase, Jak2, is activated through mutation in patients with a variety of myeloproliferative disorders (Kralovics R. et al. N Engl J Med. 2005 352:1779-90). The role of Jak3 in B and T lymphocyte maturation and T cell function makes Jak3 a target for treating transplant rejection and autoimmune diseases. Jak3 inhibitors may be useful in treating X-linked severe combined immunodeficiency, myeloproliferative disorders, transplant rejection and autoimmune diseases such as rheumatoid arthritis, inflammatory bowel syndrome, Crohn's disease, systemic lupus erythematosis, ulcerative colitis, psoriasis and multiple sclerosis.

[04371 Jnkl: Target Jnkl (i.e., c-Jun kinase 1) is a 48.3 kDa serine/threonine kinase encoded by chromosome 1 Og11.22 (symbol: MAPK8), also known as mitogen-activated protein kinase 8. Jnkl is a mitogen-activated protein kinase (i.e., MAPK) which form a family of serine-threonine protein kinases that participate in a major signaling system by which cells transduce extracellular stimuli into intracellular responses. MAPKs comprise the extracellular regulated kinases, or ERKs, for example Erk2, and the stress-activated protein kinases (SAPKs). MAPKs respond to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T- cells, Jnkl and Jnk2 are required for polarized differentiation of T-helper cells into Thl cells. Jnkl is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7 and inhibited by dual specificity phosphatases, such as DUSP1. Jnkl inhibitors may be useful in treating type 1 diabetes, type 2 diabetes, metabolic syndrome, obesity and hepatic steatosis.

[0438] Jnk2: Target kinase Jnk2 (i.e., c-Jun kinase 2) is 48.1 kDa serine/threonine kinase encoded by chromosome 5q35 (symbol: MAPK9). According to OMIM, the transcriptional activity of the c-Jun protooncoprotein is augmented through phosphorylation at two sites by c-Jun kinases (JNKs).
Using in-gel kinase assays, Hibi et al. (1993) identified 2 JNKs, 46 and 55 kD
in size. The 46-kD
protein Jnkl was shown to be a member of the mitogen-activated protein kinase (MAPK) family.
Using a JNK1 cDNA as a probe, Kallunki et al. (1994) and Sluss et al. (1994) isolated cDNAs encoding the 55-kD protein, which both designated Jnk2. Kallunki et al. (1994) reported that the sequence of the predicted 424-amino acid JNK2 protein is 83% identical to that of JNK1. Both JNKs contain a thr-pro-tyr phosphorylation motif. Northern blot analysis revealed that JNK2 is expressed as multiple transcripts in many cell types (OMIM MIM Number: 602896:
07/07/2005).

[0439] Jnk2 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Thl cells. Jnk2 isoforms display different binding patterns: alpha- 1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2, However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. Jnk2 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. Further, Jnk2 is inhibited by dual specificity phosphatases, such as DUSP1. Jnk2 inhibitors may be useful in treating atherosclerosis.

[0440] Jnk3: Target kinase Jnk3 (i.e., c-Jun kinase 3) is 52.6 kDa serine/threonine kinase encoded by chromosome 4g21-q22 (symbol: MAPK10). According to OMIM, the c-Jun kinases (JNKs) are members of the mitogen-activated protein kinase (MAPK) family that activate the Jun transcription factor. Gupta et al. (EMBO J. 1996, 15: 2760-2770) isolated brain cDNAs encoding 10 different JNK
isoforms, 8 of which were derived from either JNKI or JNK2. The other 2 cDNAs were from a gene that the authors designated JNK3. JNK3 contains an extended N-terminal region not found in JNK1 or JNK2. The 2 JNK3 isoforms, called JNK3-alpha-1 and JNK3-alpha-2, have different C termini. By SDS-PAGE of in vitro transcription/translation products, Gupta et al. (ibid.) determined that JNK3-alpha-1 migrates as a 45-to-48-kD doublet and JNK3-alpha-2 migrates as a 54-to-57-kD doublet.
They stated that the lower band probably represents translation from a second in-frame start codon that corresponds to the first codon in JNK1 and JNK2. All the JNKs were activated by treatment of cells with the inflammatory cytokine IL1. Multiple JNK isoforms were shown to be inactivated by MKP1. Comparison of the binding activity of the JNK isoforms demonstrated that they differ in their interactions with the ATF2 (CREB2), ELK1, and Jun transcription factors. Gupta et al. (ibid.) suggested that individual JNKs selectively target specific transcription factors in vivo, providing a mechanism for the generation of tissue-specific responses to the activation of the JNK signal transduction pathway. Mohit et al. (Neuron 1995, 14: 67-78) identified JNK3, or p49-3F12 kinase, as the gene encoding a 49-kD antigen found in the hippocampus and neocortex. The distribution of JNK3-expressing neurons closely matches that of Alzheimer disease targeted neurons in those areas of the brain. Northern blot analysis revealed that JNK3 is expressed as a 2.7-kb mRNA exclusively in the nervous system. Mice defective for Jnk3 are resistant to excitotoxicity induced apoptosis (Yang D.D. et al., Nature 1997, 389:865) (OMIM MIM Number: 602897: 03/13/2006).

[0441] Jnk3 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Jnk3 is required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity. Jnk3 is activated by threonine and tyrosine phosphorylation by two dual specificity kinases, MAP2K4 and MAP2K7.

phosphorylates MAPK10 on Thr-221 causing a conformational change and a large increase in Vmax.
MAP2K4 then phosphorylates Tyr-223 resulting in a further increase in Vmax.
Jnk3 is inhibited by dual specificity phosphatases, such as DUSP1.

[0442] Jnk3 inhibitors may be useful in treating inflammatory diseases including autoimmune diseases such as rheumatoid arthritis, inflammatory bowel syndrome, Crohn's disease, systemic lupus erythematosis, Sjogren's Syndrome, psoriasis and multiple sclerosis, airway inflammatory diseases such as asthma, allergy, pulmonary fibrosis, and chronic obstructive pulmonary disease, and inflammation in other organs, such as CNS inflammation, pancreatitis, nephritis, and hepatitis;
neurologic diseases such as stroke, cerebrovascular ischemia, and neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, and Huntington's disease; and neoplastic diseases such as prostate tumors and myeloid leukemia.

[0443] Kdr: Target kinase Kdr (i.e., Kinase Insert Domain Receptor) is a transmembrane tyrosine kinase of 151.5 kDa encoded by chromosome 4g12 (symbol: KDR). Kdr has a complex secondary structure comprising three Ig-like (i.e., immunoglobulin-like) domains, one IGC2 (i.e., immunoglobulin-like C2-type) domain, two additional Ig-like domains, one additional IGC2 domain, one TM (i.e., transmembrane) domain, and a split TK domain. Kdr, also known as VEGFR2 (i.e., Vascular Endothelial Growth Factor Receptor 2), Flt2, and Flkl (i.e., fetal liver kinase 1), is the receptor for Vegf and VegfC (i.e., Vascular endothelial growth factor C) and plays a key role in vascular development and regulation of vascular permeability. Walter et al.
(Genes Chromosomes Cancer, 2002, 33:295-303) has proposed based on an observed mutation in the kinase domain of KDR
that a potential mechanism involved in hemangioma formation is the alteration of the VEGF signaling pathway in endothelial and/or pericytic cells.

[0444] Due to the role of angiogenesis, and aberrant control thereof in pathologic states, Kdr is a target for therapeutic intervention. Angiogenesis is the process by which new blood vessel growth occurs from pre-existing vasculature and is mediated through multiple pro-angiogenic factors, including for example Kdr. Under normal adult physiological conditions, angiogenesis occurs during wound healing, organ regeneration, and in some aspects of female reproductive function.
Angiogenesis is also important for the progression of many pathological disorders such as solid tumor growth (ovarian, lung, breast, prancreatic, prostate, colon, gastrointestinal stromal tumor, non small cell lung cancer, and epidermoid cancer), metastasis, psoriasis, rheumatoid arthritis, diabetic retinopathy and age related macular degeneration. (Hoeben et al., 2004, Pharmacol. Rev. 56:549-580).
[0445] When the dimeric cytokine VEGF binds to the receptor tyrosine kinases Flt-1 and/or KDR, receptor dimerization occurs, followed by autophosphorylation which leads to kinase activation and phosphorylation of intracellular substrates. This receptor tyrosine kinase activity initiates a cellular signaling pathway which leads to endothelial cell proliferation and migration that is necessary for the process of angiogenesis.

[0446] Tumors that grow beyond 1-2 mm in size require the process of angiogenesis in order to receive the appropriate nutrients and oxygen that is required for tumor progression. Accordingly, inhibition of this process by small molecules that bind to the surface of receptor tyrosine kinases such as, for example, Kdr, inhibits tumor growth in both animal and human models.
Direct evidence of the role of VEGF as a tumor angiogenesis factor in vivo is shown in studies in which VEGF expression or VEGF activity was inhibited. This has been achieved with anti-VEGF antibodies, with dominant-negative VEGFR-2 mutants which inhibited signal transduction, and with anti-sense VEGF RNA
techniques. All approaches led to the reduction in tumor cell lines in vivo as a result of inhibited tumor angiogenesis. (Scappaticci., 2002, J. Clin. Oncology 20(18) :3906-3927 and references within).
[0447] Kdr inhibitors may be useful in treating solid tumor growth (e.g.
ovarian, lung, breast, prancreatic, prostate, colon, gastrointestinal stromal tumor, non small cell lung cancer, and epidermoid cancer), metastasis, psoriasis, rheumatoid arthritis, diabetic retinopathy and age related macular degeneration.

[04481 Kit. Target kinase Kit (i.e., feline Hardy-Zuckerman 4 sarcoma viral oncogene) is a 109.9 kDa transmembrane tyrosine kinase encoded by chromosome 4g12 (symbol: KIT).
Receptor protein tyrosine kinases (RPTKs) regulate key signal transduction cascades that control cellular growth and proliferation. The Stem Cell Factor (SCF) receptor Kit is a type III
transmembrane RPTK that includes five extracellular immunoglobulin (IG) domains, a single transmembrane domain, and a split cytoplasmic kinase domain separated by a kinase insert segment. Kit plays an important role in the development of melanocytes, mast, germ, and hematopoietic cells.

[04491 Stem Cell Factor (SCF) is a protein encoded by the S 1 locus, and has also been called kit ligand (KL) and mast cell growth factor (MGF), based on the biological properties used to identify it (reviewed in Tsujimura, Pathol Int 1996, 46:933-938; Loveland, et al., J.
Endocrinol 1997, 153:337-344; Vliagoftis, et al., Clin Immunol 1997, 100:435-440; Broudy, Blood 1997, 90:1345-1364; Pignon, Hermatol Cell Ther 1997, 39:114-116; and Lyman, et al., Blood 1998, 91:1101-1134.). Herein the abbreviation SCF refers to the ligand for Kit.

[0450] SCF is synthesized as a transmembrane protein with a molecular weight of 220 or 248 Dalton, depending on alternative splicing of the mRNA to encode exon 6. The larger protein can be proteolytically cleaved to form a soluble, glycosylated protein which noncovalently dimerizes. Both the soluble and membrane-bound forms of SCF can bind to and activate Kit. For example, in the skin, SCF is predominantly expressed by fibroblasts, keratinocytes, and endothelial cells, which modulate the activity of melanocytes and mast cells expressing Kit. In bone, marrow stromal cells express SCF
and regulate hematopoiesis of Kit expressing stem cells, In the gastrointestinal tract, intestinal epithelial cells express SCF and affect the interstitial cells of Cajal and intraepithelial lymphocytes.
In the testis, sertoli cells and granulosa cells express SCF which regulates spermatogenesis by interaction with Kit on germ cells, [04511 According to OMIM, Signaling from Kit is essential for primordial germ cell growth both in vivo and in vitro. Many downstream effectors of the KIT signaling pathway have been identified in other cell types, but how these molecules control primordial germ cell survival and proliferation are unknown. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells. De Miguel et al.
(2002) overcame this problem by testing the efficacy of retroviral-mediated gene transfer for manipulating gene expression in mammalian germ cells. They found that primordial germ cells can successfully be infected with a variety of types of retroviruses. They used this method to demonstrate an important role of the AKT1 in regulating primordial germ cell growth (OMIM
MIM Number:
164920: 04/17/2006).

[0452] Aberrant expression and/or activation of Kit has been implicated in a variety of pathologic states. For example, evidence for a contribution of Kit to neoplastic pathology includes its association with leukemias and mast cell tumors, small cell lung cancer, testicular cancer, and some cancers of the gastrointestinal tract and central nervous system. In addition, Kit has been implicated in playing a role in carcinogenesis of the female genital tract sarcomas of neuroectodermal origin, and Schwann cell neoplasia associated with neurofibromatosis. It was found that mast cells are involved in modifying the tumor microenvironment and enhancing tumor growth (Yang et al., J Clin Invest. 2003, 112:1851-1861; Viskochil, J Clin Invest. 2003, 112:1791-1793).

[0453] Kit inhibitors may be useful in treating malignancies, including mast cell tumors, small cell lung cancer, testicular cancer, gastrointestinal stromal tumors (GISTs), glioblastoma, astrocytoma, neuroblastoma, carcinomas of the female genital tract, sarcomas of neuroectodermal origin, colorectal carcinoma, carcinoma in situ, Schwann cell neoplasia associated with neurofibromatosis, acute myelocytic leukemia, acute lymphocytic leukemia, chronic myelogenous leukemia, mastocytosis, melanoma, and canine mast cell tumors, and inflammatory diseases, including asthma, rheumatoid arthritis, allergic rhinitis, multiple sclerosis, inflammatory bowel syndrome, transplant rejection, and hypereosinophilia.

[0454] LCK: Target kinase MCK (i.e., lymphocyte-specific protein tyrosine kinase) is a 57.9 kDa membrane associated non receptor tyrosine kinase encoded by chromosome 1p34.3 (symbol: LCK).
The protein structure comprises an SH3 and SH2 domain. LCK inhibitors may be useful in treating acute lymphoblastic leukemia, T-cell lymphoma, lymphopenia, renal carcinoma, colon carcinoma, severe combined immunodeficiency, multiple sclerosis, inflammatory bowel and type I diabetes.
[0455] MAP2K1: Target kinase MAP2K1 (i.e., Mitogen-activated protein kinase kinase 1) is a threonine/tyrosine kinase of 43.3 kDa encoded by chromosome 15g22.1-g22.33 (symbol: MAP2K1).
According to OMIM, MAP2K1 is also known as MEK1 (i.e., MAPK/ERK Kinase 1).
Mitogen-activated protein (MAP) kinases, also known as extracellular signal-regulated kinases (ERKs) are thought to act as an integration point for multiple biochemical signals because they are activated by a wide variety of extracellular signals, are rapidly phosphorylated on threonine and tyrosine residues, and are highly conserved in evolution (Crews et al., Science 1992 258: 478-480). MAP2K1 is a critical protein kinase lying upstream of MAP kinase which stimulates the enzymatic activity of MAP
kinase Crews et al. (ibid.) found that Mekl (i.e., MAP2K1) expressed in bacteria phosphorylates the Erk gene product in vitro. They showed that the Mekl gene is highly expressed in murine brain.
Seger et al. (J. Biol. Chem., 1992, 267: 25628-25631) cloned a cDNA encoding the human homolog of Mekl, symbolized MKKl by them, from a human T-cell cDNA library. When overexpressed in COS cells, the predicted 43,439-Da protein led to increased phorbol ester-stimulated MAP kinase kinase activity. They also isolated a related cDNA, called MKKlb, that appears to be an alternatively spliced form of MKK1. Seger et al. (ibid.) detected a 2.6-kb MKK1 transcript by Northern blot analysis in all tissues examined. Zheng and Guan (J. Biol. Chem., 1993, 268:
11435-11439) also cloned a human cDNA corresponding to MEK1. They noted that the 393-amino acid protein shares 99% amino acid identity with murine Mekl and 80% homology with human MEK2. The authors characterized biochemically the human MEK1 and MEK2 gene products. The gene is also symbolized MAP2K1, or PRKMK1. MAP2KI catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP
kinases and activates ERK1 and ERK2 MAP kinases. Certain inhibitors of MEK1 are potent anti-cancer agents (Sebolt-Leopold, J.S., et al., Nat. Med. 1999, 5:810) (OMIM MIM Number: 176872:
06/06/2005). MAP2K1 inhibitors may be useful in treating acute myeloid leukemia, breast, ovarian and liver cancer.

[0456] MAP2K2: Target kinase MAP2K2 (i.e., Mitogen-activated protein kinase kinase 2) is a threonine/tyrosine kinase of 44.4 kDa encoded by chromosome 7q32 (symbol:
MAP2K2); MAP2K2 is also known as Mek2; see MAP2K1 above. According to OMIM, Zheng and Guan (ibid.) isolated and sequenced 2 human cDNAs encoding members of the MAP kinase kinase (MAP2K) family, designated MEK1 and MEK2 by them. The MEK2 cDNA encodes a predicted 400-amino acid protein that shares 80% sequence identity with human MEK1. Zheng and Guan (ibid.) showed that recombinant MEK2 and MEKI both could activate human Erkl in vitro. They further characterized biochemically both MAP2Ks (OMIM MIM Number: 601263: 10/23/2003).

[0457] The mitogen-activated protein kinase (MAPK) pathway is a major pathway in the cellular signal transduction cascade from growth factors to the cell nucleus. The pathway involves kinases at two levels: MAP kinase kinases (MAPKK), and their substrates MAP (mitogen activated protein) kinases (MAPK). There are different isoforms in the MAP kinase family. [For review, see Seger, R.;
Krebs, E. G. FASEB, 9, 726, (1995)]. The compounds of this invention can inhibit the action of one or both of these kinases: MEK, a MAP kinase kinase, and its substrate ERK, a MAP kinase. ERK
(extracellular regulated kinases), a p42 MAPK, is found to be essential for cell proliferation and differentiation. Overexpression and/or over activation of MEK or ERK has been found to be associated with various human cancers [For example, Sivaraman, V. S. et al., C. C. J. Clin. Invest., 99, 1478 (1997)]. It has been demonstrated that inhibition of MEK prevents activation of ERK and subsequent activation of ERK substrates in cells, resulting in inhibition of cell growth stimulation and reversal of the phenotype of ras-transformed cells [Dudley, D. T. et al., Proc. Nat. Acad. Sci., 92, 7686 (1995)]. MAP2K2 inhibitors may be useful in treating cancer and inflammation.

[0458] MAP4K4: Target kinase MAP4K4 (i.e., Mitogen-activated protein kinase kinase 4) is a serine threonine kinase of 152.1 kDa encoded by chromosome 2g11.2 (symbol:
MAP4K4) and is also known as HGK. MAP4K4 inhibitors may be useful in treating cancer, tumor metastasis, diabetes and metabolic syndrome.

[0459] MAPKAPK2: Target kinase MAPKAPK2 (i.e., Mitogen activated protein kinase activated protein kinase 2) is 45.6 kDa serine/threonine kinase encoded by chromosome 1g32 (symbol:
MAPKAPK2). According to OMIM, Stokoe et al. (Biochem. J. 1993, 296:843-849) described a protein kinase, which they designated MAPKAP kinase-2, that was active only after phosphorylation by mitogen-activated protein kinase (MAP kinase). They identified several features that distinguish MAPKAP kinase-2 from the MAPKAP kinase-1 family. Stokoe et al. (ibid.) stated that MAPKAP
kinase-2 was identified based on its in vitro phosphorylation of glycogen synthase; however, its phosphorylation of glycogen synthase had not been shown in vivo. Stokoe et al.
(ibid.) cloned a partial human MAPKAP kinase-2 cDNA from a teratocarcinoma cell line cDNA
library. The cDNA
sequence revealed the following features (in 5-prime to 3-prime order): a proline-rich region containing 2 putative S113-binding sites, a kinase catalytic domain, a threonine residue phosphorylated by MAP kinase, and a nuclear localization signal. By Northern analysis, Stokoe et al.
(ibid.) demonstrated that the gene is expressed as a 3.3-kb transcript in all of the 6 human tissues tested. The physiological substrate of MAPKAP kinase 2 appears to be the small heat shock protein (HSP27/HSP25) (OMIM MIM Number: 602006: 03/03/2005).

[0460] In vitro, MAPKAP kinase 2 can phosphorylate glycogen synthase at Ser-7 and tyrosine hydroxylase (on Ser-19 and Ser-40). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R- X(2)-S, where Hyd is a large hydrophobic residue. MAPKAP kinase 2 is activated by two distinct pathways: the first involves the stimulation of p42/p44 MAPK by growth factors, and the second, triggered by stress and heat shock, depends on the activation of MPK2 and upstream MAPKK/MAPKKK. MAPKAPK2 inhibitors may be useful in treating cancer (e.g.
prostate, breast), stroke, menengitis, and inflammatory disorders.

[0461] Met: Target kinase Met (i.e., Hepatocyte growth factor receptor) is 155.5 kDa transmembrane tyrosine kinase encoded by chromosome 7g31 (symbol: MET).
According to OMIM, Cooper et al. (Nature 1984, 311: 29-33) cloned a transforming gene from a chemically transformed human osteosarcoma-derived cell line and mapped it to 7p11.4-qter. Identity to all previously known oncogenes except ERBB was ruled out by the fact that they are encoded by other chromosomes;
identity to ERBB is probably excluded by failure of direct hybridizations of the 2 probes. MET was the designation suggested by Cooper et al. (ibid.). Dean et al. (Nature 1985, 318: 385-388) showed that MET is in the tyrosine kinase family of oncogenes. It appeared to be most closely related in sequence to the human insulin receptor and ABL oncogene. From the sequence of MET cDNA, Park et al. (Proc. Nat. Acad. Sci. 1987, 84: 6379-6383) concluded that this oncogene is a cell-surface receptor for a then unknown ligand. The cellular MET protooncogene product is a receptor-like tyrosine kinase comprised of disulfide-linked subunits of 50 kD (alpha) and 145 kD (beta). In the fully processed Met product, the alpha subunit is extracellular, and the beta subunit has extracellular, transmembrane, and tyrosine kinase domains as well as sites of tyrosine phosphorylation (OMIM
MIM Number: 164860: 10/18/2005). Met inhibitors may be useful in treating a variety of neoplasms including kidney, breast, bladder, non-small-cell lung, colorectal, and bladder cancers, and in hepatocellular carcinoma.

[0462] MLK1: Target kinase MLK1 (i.e., mixed-lineage kinase 1, aka mitogen-activated protein kinase kinase kinase 9) is a 121.9 kDa serine/threonine kinase encoded by chromosome 14g24.3-q31 (symbol: MAP3K9). MLK1 is expressed in epithelial tumor cell lines of colon, breast and esophageal origin. Silva et al. review the mixed lineage kinase (MLK) -c-jun N-terminal kinase (JNK) signaling cascade, which leads to the phosphorylation and activation of the transcription factor c-jun. There is much evidence, from in vitro and in vivo studies, that this cascade can mediate cell death. In addition, there is evidence that it is operative upstream in the death process. It is possible that abrogation of this pathway may forestall death before irreversible cellular injury. They review the evidence that inhibition of the MLKs can prevent dopamine neuron cell death and the degeneration of their axons (Silva et al., Mov Disord 2005, 20(6):653-64). Lund et al. state that MLK
inhibitor CEP-1347 blocks the activation of the c-Jun/JNK apoptotic pathway in neurons exposed to various stressors and attenuates neurodegeneration in animal models of Parkinson's disease (PD).
Microglial activation may involve kinase pathways controlled by MLKs and might contribute to the pathology of neurodegenerative diseases. They explored the possibility that CEP-1347 modulates the microglial inflammatory response [tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and monocyte chemotactic protein-1 (MCP-1)] and report that the MLK inhibitor CEP-1347 reduced cytokine production in primary cultures of human and murine microglia, and in monocyte/macrophage-derived cell lines, stimulated with various endotoxins or the plaque forming peptide Abetal -40. Moreover, CEP-1 347 inhibited brain TNF production induced by intracerebroventricular injection of lipopolysaccharide in mice. As expected from a MLK inhibitor, CEP-1 347 acted upstream of p38 and c-Jun activation in microglia by dampening the activity of both pathways. These data imply MLKs as important, yet unrecognized, modulators of microglial inflammation, and demonstrate a novel anti-inflammatory potential of CEP-1347 (Lund et al., J
Neurochem 2005, 92(6): 1439-51). MLK1 inhibitors may be useful in treating neurodegenerative disorders such as Alzheimer's and Parkinson's disease and inflammatory disorders.

[0463] Mnkl: Target kinase Mnkl (i.e., MAP kinase interacting serine/threonine kinase 1) is a 51.3 kDa STK encoded by chromosome 1p34.1 (symbol: MKNK1). According to OMIM, Fukunaga and Hunter (EMBO J. 1997, 16:1921-1933) observed that the C-terminal region of Mnkl was phosphorylated and activated in vivo and in vitro by Erkl and p38 MAP kinases, but not by JNK/SAPK. Waskiewicz et al., (EMBO J. 1997, 16:1909-1920) reported that in vitro, Mnkl rapidly phosphorylates eIF4E at the physiologically relevant site, ser209. In cells, they observed that Mnkl is posttranslationally modified and enzymatically activated in response to mitogenic and stress stimuli.
This activation could be blocked by inhibitors of MAP kinase kinase-1 and p38, and Waskiewicz et al. (ibid.) concluded that Mnkl is downstream of multiple MAP kinases (OMIM
MIM Number:
606724: 02/27/2002).

[0464] Accordingly, dephosphorylation of eIF4E strongly correlates with inhibition or impairment of cap-dependent mRNA translation under certain stress conditions such as heat shock, nutrient deprivation, oxidative or osmotic stress, and infection of mammalian cells with certain viruses such as adenovirus (Ad) or influenza virus, among others. Mnkl inhibitors may be useful in treating conditions associated with heat shock, nutrient deprivation, oxidative or osmotic stress, infection of mammalian cells (e.g. with viruses such as adenovirus (Ad) or influenza virus), and autoimmune diseases.

[0465] p38: Target kinase p38 (i.e., Mitogen-activated Protein Kinase 14) is a 41.5 kDa STIR
encoded by chromosome 6p21.3-p21.2 (symbol: MAPK14). According to OMIM, production of interleukin-1 and tumor necrosis factor (TNF) from stimulated human monocytes is inhibited by a series of pyridinyl-imidazole compounds called CSAIDs (cytokine-suppressive antiinflammatory drugs). These agents have shown activity in a variety of animal models of acute and chronic inflammation. Using radiolabeled chemical probes for radioligand binding assays and photoaffinity labeling experiments, Lee et al. (Nature 1994, 372:739-746) identified, purified, eDNA-cloned, and biochemically characterized 2 CSBPs (CSAID-binding proteins) as molecular targets of pyridinyl-imidazole cytokine inhibitors. They designated the 2 closely related mitogen-activated protein kinases (MAPKs) CSBP1 and CSBP2. Binding of pyridinyl-imidazole compounds inhibited CSBP
kinase activity and was directly correlated with their ability to inhibit cytokine production, suggesting that the CSBPs are critical for cytokine production. Lee et al. (ibid.) considered the 2 to be products of alternative splicing. The 4.2-kb CSBP mRNA encodes a predicted 360-amino acid protein and was expressed in all tissues tested. CSBP1 and CSBP2 are identical except for a 75-nucleotide stretch within the coding region. Han et al. (Science 1994, 265: 808-811) cloned the mouse homolog as a protein that is tyrosine phosphorylated as part of the protein kinase cascades induced by endotoxic lipopolysaccharide. They named this 38-kD protein p38. As p38 is a member of the stress-activated protein kinase (SAPK) class of MAPKs, Goedert et al. (Genomics 1997, 41:501-502) referred to this protein as SAPK2A. Zervos et al. (Proc. Nat. Acad. Sci. 1995, 92:10531-10534) identified p38 as a human protein that interacts with MAX protein and designated it MXI2. The MXI2 gene encodes a 297-residue protein whose sequence indicates that it is related to the extracellular signal-regulated kinases (ERK protein kinases). MXI2 in yeast interacts with Max and with the C
terminus of c-Myc.
MXI2 phosphorylates MAX both in vitro and in vivo. The authors speculated that phosphorylation by MXI2 may effect the ability of MAX to oligomerize with itself and its partners, bind DNA, or regulate gene expression (OMIM MIM Number: 600289: 02/13/2006).

[0466] There are four known isoforms of p38, i.e., p38-u, p380, p38-Y, and p386. Theaando isoforms are expressed in inflammatory cells and are key mediators of TNF-a production. Inhibiting the p38a and (3 enzymes in cells results in reduced levels of TNF-a expression. Also, administering p38a and (3 inhibitors in animal models of inflammatory disease has proven that such inhibitors are effective in treating those diseases. Accordingly, the p38 enzymes serve an important role in inflammatory processes mediated by IL-1 and TNF-a. Compounds that reportedly inhibit p38 kinase and cytokines such as IL-1 and TNF-a for use in treating inflammatory diseases are disclosed in the following published international patent applications: WO 00/12497 (quinazoline derivatives as p38 kinase inhibitors); WO 00/56738 (pyridine and pyrimidine derivatives for the same purpose); WO
00/12497 (discusses the relationship between p38 kinase inhibitors); and WO
00/12074 (piperazine and piperidine compounds useful as p38 inhibitors).

[0467] p38 responds to activation by environmental stress, pro- inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5.
Additionally, p38 plays a critical role in the production of some cytokines, for example IL-6, p38 phosphorylates ELK1 and ATF2.

[0468] p38 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K3 or MAP2K6, and potentially also MAP2K4 and it is inhibited by dual specificity phosphatases, such as DUSP1. p38 is specifically inhibited by the binding of pyridinyl-imidazole compounds, which are cytokine- suppressive anti-inflammatory drugs (CSAID).

[0469] p38 inhibitors have the potential to treat a number of diseases, including, but not limited to acute coronary syndrome, stroke, atherosclerosis, and inflammatory diseases such as rheumatoid arthritis, inflammatory bowel disease, and Crohn's disease.

[0470] PDGFR kinase family: Related to Fms and Kit are two platelet-derived growth factor receptors, alpha (i.e., PDGFRA) and beta (PDGFRB). The gene coding for PDGFRA
is located on chromosome 4g12 in the same region of chromosome 4 as the oncogene coding for Kit. Most gastrointestinal stromal tumors (GIST) have activating mutations in Kit, and most patients with GISTs respond well to Gleevec, which inhibits Kit. Heinrich et al. (Science 2003, 299:708-10.) have shown that approximately 35% of GISTs lacking Kit mutations have intragenic activation mutations in the gene encoding pdgfra, and that tumors expressing Kit or PDGFRA were indistinguishable with respect to activation of downstream signaling intermediates and cytogenetic changes associated with tumor progression. Thus, Kit and PDGFRA mutations appear to be alternative and mutually exclusive oncogenic mechanisms in GISTs. PDGF is a potent growth factor and chemoattractant for smooth muscle cells (SMCs), and the renarrowing of coronary arteries following angioplasty is due in part to the enhanced proliferation of SMCs in response to increased levels of PDGF. Therefore, compounds that inhibit the kinase activity of PDGFr may be useful in the treatment of restenosis. In addition, since PDGF and PDGFr are overexpressed in several types of human gliomas, small molecules capable of suppressing PDGFr activity have potential utility as anticancer therapeutics [Nister, M., J. Biol. Chem., 266, 16755 (1991); Strawn, L. M., J. Biol. Chem.
269, 21215 (1994)].
[0471] PDGFRoz: Target PDGFRC (i.e., Plate Derived Growth Factor Receptor, alpha) is a 122.7 kDa transmembrane tyrosine kinate encoded by chromosome 4q12 (symbol: PDGFRA).
According to OMIM, The KIT oncogene, another member of the PDGF growth factor receptor subfamily, is located in the same region of chromosome 4 (Stenman et al., Genes Chromosomes Cancer 1989, 1:155-158). PDGFR1 (i.e.PDGFRB) and CSF1R (i.e. FMS) are also membrane-spanning growth factor receptors with tyrosine kinase activity. The PDGFRI and CSF1R genes appear to have evolved from a common ancestral gene by gene duplication, inasmuch as these 2 genes are tandemly linked on chromosome 5 (Roberts et al., Cell 1988, 55: 655-661). They are oriented head-to-tail with the 5-prime exon of FMS located only 500 bp from the last 3-prime exon of PDGFRB. An analogous situation may exist for the PDGFR2 (i.e. PDGFRA) and KIT genes on chromosome 4. From an evolutionary point of view, it is possible that the distribution of these 4 loci, PDGFR2, KIT, PDGFR1, and FMS, on chromosomes 4 and 5 is a result of gene duplication and chromosome doubling (tetraploidization) (OMIM MIM Number: 173490: 03/21/2005). PDGFRA inhibitors may be useful in treating idiopathic hypereosinophilic syndrome, chronic eosinophilic leukemia, glioma, gastrointestinal stromal tumors (GISTs), juvenile myelomonocytic leukemia, metastatic medulloblastoma, atherogenesis, and restenosis.

[0472] PDGFRR: Target PDGFR(3 (i.e., Plate Derived Growth Factor Receptor, beta) is a 124.0 kDa transmembrane tyrosine kinate encoded by chromosome 5g31-q32 (symbol:
PDGFRB).
According to OMIM, stimulation of cell proliferation of the receptor for PDGF
has been implicated in atherogenesis and in cell transformation by the SIS oncogene. Escobedo et al.
(1986) sequenced the receptor and cloned its gene. Gronwald et al. (Proc. Nat. Acad. Sci. 1988, 85:3435-3439) cloned a cDNA coding for human PDGFR and studied its expression. The cDNA contained an open reading frame that coded for a protein of 1,106 amino acids. In transfectants, Gronwald et al. (ibid.) found that the PDGFR clone expressed a high affinity receptor specific for the BB
isoform of PDGF, i.e., PDGF dimers composed of 2 B chains. There may be a separate class of PDGF
receptor that binds both the homodimers and the heterodimer. Claesson-Welsh et al. (Molec. Cell.
Biol. 1988, 8:3476-3486) determined the structure of the human PDGF receptor as deduced from a full-length cDNA
clone. The receptor expressed in Chinese hamster ovary cells was found to bind specifically to B-chain-containing PDGF molecules. With the description of a second PDGF
receptor, it is necessary to use the symbol PDGFR1. Matsui et al. (1989) designated the second type of PDGFR as type alpha because PDGF binding was blocked by AA as well as BB isoforms of the ligand;
the product of the earlier cloned PDGF receptor was termed type beta (OMIM MIM Number: 173410:
11/19/2003).
[0473] PDGFR(3 has been implicated in hematological cancers, such as chronic myelomonocytic leukemia (CMML), in which a significant number of patients have a t(5;12)(g33;pl3) translocation resulting in TEL-PDGFR(3 fusion protein. Golub et al. report that the consequence of the t(5; 12) translocation is expression of a fusion transcript in which the tyrosine kinase domain of the platelet-derived growth factor receptor beta (PDGFR(3) on chromosome 5 is coupled to a novel ets-like gene, tel, on chromosome 12. The tel-PDGFR beta fusion demonstrates the oncogenic potential of PDGFR/(3 and may provide a paradigm for early events in the pathogenesis of AML (Golub et al., Cell 1994, 77:307-316). PDGFRB inhibitors may be useful in treating idiopathic hypereosinophilic syndrome, chronic eosinophilic leukemia, juvenile myelomonocytic leukemia, and metastatic medulloblastoma.

[0474] PDPK1: Target PDPK1 (3-phosphoinositide dependent protein kinase-1) is a 63.2 kDa serine/threonine kinase encoded by chromosome 16p13.3 (symbol: PDPK1). PDPK1 inhibitors may be useful in treating cancers and diabetes.

[0475] Piml: Target Piml (i.e., Proviral Integration Site 1) is a 35.7 kDa serine/threonine kinase encoded by chromosome 6p21.2 (symbol: PIM1) found in the cytoplasm and nucleus. The structure of Piml comprises a STK (i.e., serine/threonine kinase) domain. X-ray crystal structures of Piml bound to various small molecules have recently been solved (Jacobs, et al., J
Biol Chem 2005 280:
13728-34; Qian, et al., J Biol Chem 2005 280: 6130-7; Kumar, et al., J Mol Biol 2005 348: 183-93).
[0476] Piml is the first described member of a unique family of serine/threonine kinases, which includes at least two other kinases (PIM2 and PIM3) with significant sequence homology to Piml (van der Lugt, et al., Embo J 1995 14: 2536-44; Feldman, et al., J Biol Chem 1998 273: 16535-43). The PIM1 protooncogene was originally identified as a genetic locus frequently activated by the proviral insertion of Moloney murine leukemia virus into mouse T cell lymphomas (Cuypers et al., Cell 1984, 37:141-150). Several substrates of Piml phosphorylation have been identified, including c-Myb (Winn, et al., Cell Cycle 2003 2: 258-62), BAD (Yan, et al., J Biol Chem 2003 278: 45358-67; Aho, et al., FEBS Lett 2004 571: 43-9), SOCS-1 (Chen, et al., Proc Natl Acad Sci U
S A 2002 99: 2175-80), Cdc25A (Mochizuki, et al., J Biol Chem 1999 274: 18659-66), HP1 (Koike, et al., FEBS Lett 2000 467: 17-21), PAP-1 (Maita, et al., Eur J Biochem 2000 267: 5168-78), p2lcip'/Wan (Wang, et al., Biochim Biophys Acta 2002 1593: 45-55), PTP-U2S (Wang, et al., Arch Biochem Biophys 2001 390:
9-18), and NFATc1 (Rainio, et al., J Immunol 2002 168: 1524-7). Piml has been shown to have diverse biological roles in cell survival, proliferation, differentiation, and immune response (Wang, et al., J Vet Sci 2001 2: 167-79; Bachmann, et al., Int J Biochem Cell Biol 2005 37: 726-30). However, mice lacking all three Pim genes have recently been shown to be viable and demonstrate that the PIM
kinases are important for growth factor signaling, but are not essential for development (Mikkers, et al., Mol Cell Biol 2004 24: 6104-15). During embryonal development PIM genes are expressed in partially overlapping fashion in cells in both immune and central nervous system as well as in epithelia (Eichmann A, Yuan L, Breant C, Alitalo K, and Koskinen PJ. (2000) Developmental expression of PIM kinases suggests functions also outside of the hematopoietic system. Oncogene 19:
1215-1224). PIM-1, the prototypical member of the PIM family is located both in the cytoplasm and nucleus, but its precise role in these two locations has not been fully elucidated.

[0477] Dysfunction of Piml has been implicated in the progression of multiple cancers, including several hematopoietic and prostate cancers. Although the exact mechanisms by which Piml participates in cell transformation have not been completely elucidated, several reports point to the ability of Piml to prolong cell survival (Lilly, et al., Cancer Res 1997 57:
5348-55; Lilly, et al., Oncogene 1999 18: 4022-31; Moroy, et al., Proc Natl Acad Sci U S A 1993 90:
10734-8).
Overexpression of Piml has been observed in myeloid and lymphoid acute leukemia and Piml is constitutively expressed in some myeloid leukemia cell lines (Lilly, et al., Oncogene 1992 7: 727-32;
Amson, et al., Proc Natl Acad Sci U S A 1989 86: 8857-61). Increased Piml expression has also been identified in neoplastic prostate cancer specimens from patients by cDNA
microarray analysis and by anti-Piml antibody staining (Dhanasekaran, et al., Nature 2001 412: 822-6). In a transgenic murine model of prostate cancer in which human c-myc is expressed, the gene expression profile is consistent with that seen in human prostate cancer, including upregulation of Piml (Ellwood-Yen, et al., Cancer Cell 2003 4: 223-38). In addition, Piml may participate in deregulated cell growth in prostate cancer through the hormone independent activation of the androgen receptor, a typical characteristic of advanced prostate cancer that offers poor patient prognosis (Kim, et al., Oncogene 2004 23: 1838-44).
The PIM-1 proto-oncogene has also been implicated in human hematopoietic malignancies with its overexpression frequently detected in human hematopoietic cell lines as well as in fresh tumor cells from patients with leukemia (Nagarajan et al. Proc. Natl. Acad. Sci. USA, 1986, 83:2556-2560;
Meeker et al., Oncogene Res. 1987, 1: 87-101; Amson et al., Proc. Natl. Acad.
Sci. USA, 1989, 86:
8857-8861).

[0478] In diffuse large cell lymphoma (DLCL), the most common form of non-Hodgkin's lymphoma, Piml has been shown to undergo chromosomal translocations, resulting in its overexpression (Akasaka, et al., Cancer Res 2000 60: 2335-41). A recent study showed that Piml was also the target of an aberrant somatic hypermutation in DLCL (Pasqualucci, et al., Nature 2001 412: 341-6). Hypermutation sites are distributed in both 5' UTR and coding sequence, and independent of the chromosomal translocations. Notably, there are seven missense mutations introduced into the coding exons of the gene. These missense mutations may affect the three-dimensional structure and, in some cases, the kinase activity of the Piml protein. Hypermutations are also detected in Piml found in primary central nervous system lymphomas (Montesinos-Rongen, et al., Blood 2004 103: 1869-75) and multiple subtypes of AIDS-induced non-Hodgkin's lymphomas (Gaidano, et al., Blood 2003 102: 1833-41). Inhibition of Piml kinase activity by small molecules has the potential to offer a therapeutic benefit in these diseases.

[0479] Transgenic mice with PIM-1 driven by Emu enhancer sequences demonstrated that PIM-1 function as a weak oncogene because by itself it does not lead to tumor formation but does so after a second oncogenic gene become overexpressed. In 75% of the tumors over-expressing PIM-1, the second gene found to be over-expressed is c-myc (van der Houven van Oordt CW, Schouten TG, van Krieken JH, van Dierendonck JH, van der Eb AJ, Breuer ML. (1998) X-ray-induced lymphomagenesis in E mu-PIM-1 transgenic mice: an investigation of the co-operating molecular events.
Carcinogenesis 19:847-853). In fact when crosses were made between Emu-PIM
transgenic mice and Emu-myc transgenic mice, the combination of genes is so oncogenic that the offsprings die in utero due to pre B cell lymphomas (Verbeek S, van Lohuizen M, van der Valk M, Domen J, Kraal G, and Berns A. (1991) Mice bearing the Emu-myc and Emu-PIM-1 transgenes develop pre-B-cell leukemia prenatally. Mol. Cell. Biol., 11: 1176-1179).

[0480] Mice deficient for PIM-1 show normal synaptic transmission and short-term plasticity but failed to consolidate enduring LTP (i.e., long term potentiation) even though P11\4-2 and PIM-3 are expressed in the hippocampus (Konietzko U, Kauselmann G, Scafidi J, Staubli U, Mikkers H, Berns A, Schweizer M, Waltereit R, and Kuhl D.(1 999) PIM kinase expression is induced by LTP
stimulation and required for the consolidation of enduring LTP. EMBO J. 18:
3359-3369).
[0481] Various factors are known to enhance the transcription of PIM-1 kinase in mouse and human. PIM-1 closely cooperates with another oncoprotein, c-myc, in triggering intracellular signals leading to both transformation and apoptosis and the selective inhibition of apoptotic signaling pathways leading to Bcl-2 (van Lohuizen M, Verbeek S, Krimpenfort P, Domen J, Saris C, Radaszkiewicz T, and Berns A. (1989) Predisposition to lymphomagenesis in PIM-1 transgenic mice:
cooperation with c-myc and N-myc in murine leukemia virus-induced tumors. Cell 56:673-682;
Breuer ML, Cuypers HT, Berns A. (1989). Evidence for the involvement of PIM-2, a new common proviral insertion site, in progression of lymphomas. EMBO J. 8:743-748.;
Verbeek S, van Lohuizen M, van der Valk M, Domen J, Kraal G, and Berns A. (1991) Mice bearing the E mu-myc and E mu-PIM-1 transgenes develop pre-B-cell leukemia prenatally. Mol. Cell. Biol. 11:
1176-1179; Shirogane T, Fukada T, Muller JM, Shima DT, Hibi M, and Hirano T. (1999) Synergistic roles for PIM-1 and c-Myc in STAT3-mediated cell cycle progression and antiapoptosis. Immunity, 11:
709-719). PIM-1 kinase is induced by T cell antigen receptor cross linking by cytokines and growth factors and by mitogens including IL2, IL3, IL6, IL9, 11,12, 11,15, GM-CSF, G-CSF, IFNa, INFg, prolactin, ConA, PMA and anti-CD3 antibodies (Zhu N, Ramirez LM, Lee RL, Magnuson NS, Bishop GA, and Gold MR.(2002) CD40 signaling in B cells regulates the expression of the PIM-1 kinase via the NF-kappa B pathway. J Immunol. 168: 744-754). PIM-1 expression is rapidly induced after cytokine stimulation and the proliferative response to cytokines is impaired in cells from PIM-1 deficient mice (Domen J, van der Lugt NM, Acton D, Laird PW, Linders K, Berns A.(1993) PIM-1 levels determine the size of early B lymphoid compartments in bone marrow. J. Exp. Med. 178: 1665-1673).

[0482] Members of the PIM family of kinases interact with Socs-1 protein, a potent inhibitor of JAK activation thereby playing a major role in signaling down stream of cytokine receptors. The phosphorylation of Socs-1 by PIM family of kinases prolongs the half-life of Socs-1 protein, thus potentiating the inhibitory effect of Socs-1 on JAK-STAT activation (Chen XP, Losman JA, Cowan S, Donahue E, Fay S, Vuong BQ, Nawijn MC, Capece D, Cohan VL, Rothman P.
(2002) PIM
serine/threonine kinases regulate the stability of Socs-1 protein. Proc. Natl.
Acad. Sci. USA 99:2175-2180.). PIM-1 is expressed during GUS phase of the cell cycle suggesting that it is involved in cell cycle regulation (Liang H, Hittelman W, Nagarajan L., Ubiquitous expression and cell cycle regulation of the protein kinase PIM-1. (1996) Arch Biochem Biophys. 330:259-265). ). PIM-1 kinase activity and the protein level is increased in CD 40 mediated B cell signaling and this increase in PIM-1 level is mediated through the activation of NF-kB (Zhu et al. 2002.
supra). PIM-1 can physically interact with NFATc transcription factors enhancing NFATc dependant transactivation and IL2 production in Jurkat cells (Rainio EM, Sandholm J, Koskinen PJ. (2002) Cutting edge:
Transcriptional activity of NFATcl is enhanced by the PIM-1 kinase. J.
Immunol. 168:1524-1527).
This indicates a novel phosphorylation dependant regulatory mechanism targeting NFATc1 through which PIM-1 acts as down stream effector of ras to facilitate IL2 dependant proliferation and survival of lymphoid cells (ibid.).

[0483] Piml is shown to interact with many other targets. Phosphorylation of Cdc25A phosphatase, a direct transcriptional target of c-myc, increase its phosphatase activity both in-vivo and in-vitro indicating that Cdc25A link PIM-1 and c-myc in cell transformation and apoptosis (Mochizuki T, Kitanaka C, Noguchi K, Muramatsu T, Asai A, and Kuchino Y. (1999) Physical and functional interactions between PIM-1 kinase and Cdc25A phosphatase. Implications for the PIM-1 -mediated activation of the c-Myc signaling pathway; J. Biol. Chem. 274:18659-18666).
PIM-1 also phosphorylate PTP-U2S, a tyrosine phosphatase associated with differentiation and apoptosis in myeloid cells, decreasing its phosphatase activity and hence preventing premature onset of apoptosis following PMA-induced differentiation (Wang et al. (2001) Pim-1 negatively regulates the activity of PTP-U2S phosphatase and influences terminal differentiation and apoptosis of monoblastoid leukemia cells. Arch. Biochem. Biophys. 390:9-18). The phosphorylation of another PIM-1 target, heterochromatin protein 1 (HP 1) has been shown to be involved in transcription repression (Koike et al., FEBS Lett. 2000, 467: 17-21).

[0484] Piml inhibitors may be useful in treating cancers such as hematopoietic (e.g. acute myeloid and acute lymphoid leukemias) and prostate cancers, and non-Hodgkin's lymphomas.

[0485] Pim2: Target kinase Pim2 (i.e., Serine/threonine-protein kinase Proviral Integration Site 2) is a 34.2 kDa STK encoded by chromosome Xpl 1.23 (symbol: PIM2). Pim2 has also been shown to play a role in cell survival and the control of apoptosis and its value as an inhibitor target has been considered (Yan, et al., JBiol Cohen 2003 278: 45358-67; Giles, Blood 2005 105: 4158-4159; Fox, et al., Genes Dev 2003 17: 1841-54). Pim2 has been found to be overexpressed in some lymphomas (Cohen, et al., Leuk Lymphoma 2004 45: 951-5). Additionally, Pim2 is required for the rapamycin resistant T-cell survival and the rapamycin-resistant growth of nontransformed hematopoietic cells (Hammerman, et al., Blood 2005 105: 4477-83; Fox, et al., JExp Med 2005 201:
259-66). Pim2 inhibitors maybe useful in treating lymphomas.

[0486] Pim3: Target kinase Pim3 (i.e., Serine/threonine-protein kinase Proviral Integration Site 3) is a 35.8 kDa STK encoded by chromosome 22g13 (symbol: PIM3). Pim3 has recently been shown to be overexpressed in human hepatocellular carcinoma cells and its ablation resulted in attenuated cell proliferation and enhanced apoptosis, suggesting that Pim3 can also participate in abnormal cell growth and inhibition of apoptosis (Fujii, et al., Int J Cancer 2005 114: 209-18). Pim3 inhibitors may be useful in treating hepatocellular carcinoma.

[0487] PKC alpha: Target kinase PKC alpha (i.e., Protein kinase C alpha) is a 76.8 kDa STK
encoded by chromosome 17q22-q23.2 (symbol: PRKCA). Protein kinase C (PKC) is the major phorbol ester receptor. Nine mammalian members of the PKC family have been identified and designated alpha, beta, gamma, delta, epsilon, zeta, eta, theta, and lambda.
According to OMIM, Parker et al.-(Science 1986, 233:853-859) purified PKC from bovine brain and through the use of oligonucleotide probes based on partial amino acid sequence, derived cDNA
clones from bovine cDNA libraries. Activation of PKC by calcium ions and the second messenger diacylglycerol is thought to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli.
Birnbaum et al. (Science 2004, 306:882-884) showed that high levels of PKC activity in prefrontal cortex, as seen for example during stress exposure, markedly impaired behavioral and electrophysiologic measures of working memory. Birnbaum (ibid.) concluded that excessive PKC activation can disrupt prefrontal cortical regulation of behavior and thought, possibly contributing to signs of prefrontal cortical dysfunction such as distractibility, impaired judgment, impulsivity, and thought disorder (OMIM MIM Number:
176960: 04/17/2006). A mutation in PKC-alpha (D294G) correlates with pituitary tumor. PKC alpha inhibitors may be useful in treating pituitary tumors and prefrontal cortical dysfunction such as distractibility, impaired judgment, impulsivity, and thought disorder, also may be used to sensitize chemotherapy in breast, colon, and non small cell lung cancers.

[0488] PKC beta: Target kinase PKC beta (i.e., Protein kinase C, beta 1) is a 76.7 kDa STK
encoded by chromosome 16p11.2 (symbol: PRKCB1). According to OMIM, Leitges et al. (Science 1996, 273:788-791) found that mice homozygous for a targeted disruption of the PRKCB1 gene develop an immunodeficiency characterized by impaired humoral immune responses and reduced cellular responses of B cells similar to X-linked immunodeficiency (Xid) in mice. Thus, they concluded that the 2 isoforms, PKC-beta-I (PRKCB1) and PKC-beta-II (PRKCB2), play an important role in B-cell activation and may be functionally linked to Bruton tyrosine kinase in antigen receptor-mediated signal transduction (OMIM MIM Number: 176970: 03/03/2006). In general, inhibitors PKC beta and PKC isoforms may be effective in treating disorders characterized by dysregulated NFKB survival signaling. PKC beta inhibitors may be useful in treating diabetic retinopathy.

[0489] PKC theta: Target kinase PKC-theta (i.e., Protein kinase, theta) is a 81.9 kDa STK encoded by chromosome 1Op15 (symbol: PRKCQ). According to OMIM, in an attempt to find PKC isoforms that are involved in growth control and/or activation of T lymphocytes, Baier et al., (J. Biol. Chem.
1993, 268:4997-5004) used a human peripheral blood lymphocyte-derived cDNA
library was employed to identify a novel PKC isoform, termed PKC-theta. The gene encodes a protein of approximately 80 kD, expressed predominantly in lymphoid tissues and hematopoietic cell lines, particularly T cells. The alpha form (PRKCA) has been mapped to chromosome 17, the beta form (PRKCBI) to chromosome 16, the gamma form (PRKCG) to chromosome 19, and the delta form (PRKCD) to chromosome 3. By fluorescence in situ hybridization, Erdel et al.
(Genomics 1995, 25:595-597) assigned the PRKCQ gene to 1Opl5. Blanco and Brown (Mammalian Genome 1997, 8:70-71) mapped the homolog Pkcq to mouse chromosome 2 by analysis of an interspecific backcross. Sun et al. (Nature 2000, 404:402-407) demonstrated that PKC-theta is essential for T-cell antigen receptor (TCR)-mediated T-cell activation but dispensable during TCR-dependent thymocyte development. They generated mice deficient in Pkc-theta by homologous recombination. Mutant mice were normal and fertile. TCR-initiated NF-kappa-B activation was absent from PKC-theta -/-mature T lymphocytes, but was intact in thymocytes. Activation of NF-kappa-B
by tumor necrosis factor-alpha and interleukin-1 was unaffected in the mutant mice. Induction of JNK was normal in T
cells from mutant mice. Sun et al. (ibid.) concluded that PKC-theta functions in a unique pathway that links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes.
Using hyperinsulinemic-euglycemic clamps, Kim et al. Q. Clin. Invest. 2004, 114:823-827) demonstrated that skeletal muscle and hepatic insulin action did not differ between wildtype and Pkc-theta null mice. A 5-hour lipid infusion decreased insulin-stimulated skeletal muscle glucose uptake in the wildtype mice that was associated with 40 to 50% decreases in insulin-stimulated tyrosine phosphorylation of insulin receptor substrate-1 (IRS 1) and IRS 1 -associated P13K activity. In contrast, Pkc-theta inactivation prevented fat-induced defects in insulin signaling and glucose transport in skeletal muscle. Kim et al. (ibid.) concluded that PKC-theta is a crucial component mediating fat-induced insulin resistance in skeletal muscle (OMIM MIM Number: 600448:
10/15/2004).

[0490] Li et al. report that PKC plays a critical role in competitive activity-dependent synapse modification at the neuromuscular synapse in vitro and in vivo. This action involves a reduction of the strength of inactive inputs to muscle cells that are activated by other inputs. A decrease of postsynaptic responsiveness and a loss of postsynaptic acetyl choline receptors account for the heterosynaptic loss in vitro. The loss is not seen in preparations in which PKC has been blocked pharmacologically. Here, they show that the loss does not occur in in vitro preparations made from animals genetically modified to lack the theta isoform of PKC. Synapse elimination in the newborn period in vivo is delayed but is eventually expressed in knock-out animals.
PKC-dependent synapse reduction is suppressed in heterologous cultures combining normal nerve and PKC-theta-deficient muscle, as might be expected from the postsynaptic locus of the changes that underlie the activity-dependent plasticity. Preparations in which PKC-theta-deficient neurons innervated normal muscle also exhibited a marked deficit in PKC-deficient synapse reduction. The presynaptic action of PKC-theta implied by this observation is blocked by TTX, and the authors propose that the activity-related synapse strengthening is decreased by presynaptic PKC-theta. Thus, PKC-theta in both presynaptic and postsynaptic elements plays a critical role in activity-dependent synapse modulation and loss (Li et al., Journal of Neuroscience 2004, 24(15):3762-3769). PKC theta inhibitors may be useful in treating insulin resistance, T-cell lymphoma.

[0491] Plkl: Target kinase Plkl (i.e., Polo like kinase 1) is a 68.3 kDa STK
encoded by chromosome 16p12.3 (symbol: PLK1). Plkl is a regulator required for multiple mitotic processes, including bipolar mitotic spindle formation, actin ring formation, and chromosomal segregation.
According to OMIM, Holtrich et al. (Proc. Nat. Acad. Sci. 1994, 91:1736-1740) observed that PLK1 transcripts are present at high level in tumors of various origins. In vertebrate cells, the nuclear entry of mitosis-promoting factor (MPF) during prophase is thought to be essential for the induction and coordination of M -phase events. Phosphorylation of cyclin B 1 is central to its nuclear translocation.

"1'oyoshima-Morimoto et al. (Nature 2001, 410:215-220) purified a protein kinase from Xenopus M-phase extracts that phosphorylates a crucial serine residue (5147) in the middle of the nuclear export signal sequence of cyclin B1. They identified this kinase as Plxl, a Xenopus homolog of PLK1.
During cell cycle progression in HeLa cells, a change in the kinase activity of endogenous Plsl toward S147 and/or S133 correlates with a kinase activity in the cell extracts. An anti-PLK1 antibody depleted the M-phase extracts of the kinase activity toward S147 and/or S133.
An anti-phospho-5147 antibody reacted specifically with cyclin B1 only during G2/M phase. A mutant cyclin B1 in which S 133 and S 147 were replaced by alanines remained in the cytoplasm, whereas wildtype cyclin B 1 accumulated in the nucleus during prophase. Further, coexpression of constitutively active Plkl stimulates nuclear entry of cyclin B1. Toyoshima-Morimoto et al. (ibid.) concluded that Plkl may be involved in targeting MPF to the nucleus during prophase (OMIM MIM Number:
602098:
08/05/2005). Plk1 inhibitors may be useful in treating cancers (e.g. lymphoma of the thyroid, non-Hodgkin's lymphomas, colorectal cancers, leukemias and melanoma), also useful as sensitizer in chemotherapy.

[0492] Pyk2: Target kinase Pyk2 (i.e., Protein-Tyrosine Kinase 2) is a 115.9 kDa tyrosine kinase of the FAK family (see e.g., target Fak) encoded by chromosome 8p2l.1 (symbol:
PTK2B). As with target kinase Fak, Pyk2 comprises B41 and TK domains, and Pyk2 is also known as Fak2 (i.e., Focal Adhesion Kinase 2). Because Pyk2 is calcium dependent, it is also known as CADAK (i.e., calcium-dependent tyrosine kinase).

[0493] As mentioned, another member of the FAK subfamily is kinase Fak. Pyk2 and Fak shares 65% sequence identity in the kinase domain and have similar domain structure:
an N-terminus domain for integrin binding, and a C-terminus domain for Paxillin binding. Fak is ubiquitously expressed while Pyk2 exhibits a more restricted tissue expression pattern primarily in neuronal and hematopoetic tissues.

[0494] According to OMIM, Focal adhesion kinases (i.e., FAKs) are cytoplasmic protein-tyrosine kinases associated with focal adhesions and whose activity is induced by ligand binding to various receptors including those of, for example, integrin and growth factors. FAKs are known to target paxillin and are substrates for Src family kinases (Calalb et al., Molec.
Cell. Biol. 1995, 15:954-963).
Herzog et al. (Genomics 1996, 32:484-486) identified a gene for another focal adhesion kinase by low-stringency screening of a hippocampus cDNA library. They symbolized the gene FAK2. The FAK2 cDNA encodes a predicted 1,009-amino acid protein with 42% identity to FAK1. Northern blot analysis detected a 4.5-kb mRNA in brain, kidney, spleen, and lymphocytes. Protein-tyrosine kinases in the central nervous system are activated in response to a variety of neurotrophic factors that control neuronal differentiation and survival via cell surface receptors.
Also, protein phosphorylation is involved in membrane excitability and the function of ion channels. Lev et al. (Nature 1995, 376:737-745) discovered a nonreceptor type protein kinase that is highly expressed in adult rat brain.
The kinase, which they symbolized PYK2 (proline-rich tyrosine kinase-2), was cloned from a rat spinal cord cDNA library using degenerate PCR primers corresponding to conserved tyrosine kinase motifs of PYKI (see Manser et al., Nature 1993, 363:364-367). Lev et al.
(ibid.) cloned the human homolog from a human fetal brain cDNA library using the rat sequence as a probe. The predicted protein of 1,009 amino acids has 61% sequence identity to the FAK1 protein (Ptk2) (OMIM MIM
Number: 601212: 01/19/2005). PKC-theta may represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. PKC-theta interacts with the SH2 domain of Grb2, and may phosphorylate the voltage-gated potassium channel protein Kvl.2.
Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity.

[0495] Pyk2 regulates multiple signaling events crucial for macrophage morphology and migration.
It mediates the Jak-dependent activation of MAPK and Statl. By rapidly translocating to the vicinity of the immune synapse after T cell receptor stimulation, Pyk2 plays an essential role in T cell activation and polarized secretion of cytokines. The morphology and behavior of macrophages in Pyk2-/- mice were impaired. Macrophages isolated from mutant mice failed to become polarized, to undergo membrane ruffling, and to migrate in response to chemokine stimulation. Moreover, the contractile activity in the lamellipodia of Pyk2-/- macrophages was impaired, as revealed by measuring the rearward movement toward the nucleus of fibronectin-coated beads on the lamellipodia in opposition to an immobilizing force generated by optical tweezers.

[0496] Pyk2 is implicated in several therapeutic areas including inflammation (e.g. osteoporosis, Polycystic Kidney Disease, rheumatoid arthiritis and some bowel diseases) and CNS disease like Parkinson's disease and Alzheimer's disease). Pyk2 in osteoclasts is an adhesion kinase, localized in the sealing zone, activated by ligation of v3 integrin, and phosphorylated by Src kinase. Methods for preventing cell death in a subject and their application in the treatment of neurodegenerative diseases and conditions, such as Alzheimer's disease, stroke, Parkinson's disease have been pattened by Griswold-Prenner Irene and Powell Kyle.

[0497] Pyk2 is also a potential therapeutic target for tumors. Pyk2 is a novel effector of fibroblast growth factor receptor 3 activation. Pyk2 facilitates EGFR- and c-Src-mediated Stat3 activation and has a role in triggering Stat3-induced oncogenesis. HER3, but not HER2, mediates the phosphorylation of the C-terminal region of PYK2 to promote a mitogenic response through activation of the MAPK pathway. Furthermore, PYK2 phosphorylation by HER3 induces tumor invasion. A central role of PYK2 in signaling downstream of HER3 is substantiated by the demonstration that expression of a dominant-negative PYK2-KM construct abrogates the Heregulin-induced MAPK activity and inhibits the invasive potential of glioma cells.

[0498] Overexpression of wild-type RAFTK significantly enhanced breast cancer cell invasion, while overexpression of the mutants Tyr402 or Tyr881 of RAFTK inhibited this migration. Therefore, Pyk2 may serve as a mediator and an integration point between focal adhesion molecules in HRG-mediated signaling in T47D breast cancer cells.

[0499] A murine pancreatic cancer cell line overexpressing Pyk2, mPanc02, was treated with a Pyk2-dominant negative adenovirus (Ad-Pyk2DN), or GFP (ad-GFP) adenovirus. The dominant negative Pyk2 adenovirus is able to decrease tumor growth and increase survival in several in vivo tumor models.

[0500] Although no point mutation of Pyk2 has been reported to be significant in any disease, human umbilical vein endothelial cells express mRNA transcripts for both the full length isoform Pyk2 and the truncated isoform Pyk2-H containing the C-terminal deletion.

[0501] Pyk 2 inhibitors may be useful in treating inflammation (e.g.
osteoporosis, polycystic kidney disease, rheumatoid arthiritis and inflammatory bowel disease), CNS disease (e.g. Parkinson's disease and Alzheimer's disease), stroke and cancers (e.g. gliomas, breast cancer, and pancreatic cancer).
[0502] Ret: Target Ret (i.e., Rearranged during Transfection) is a 124.3 kDa tyrosine kinase encoded by chromosome l Oql 1.2 (symbol: RET). Ret is also known as c-ret (i.e., cellular ret). The domain structure of Ret comprises cadherin, transmembrane, and TK domains.
Cadherins are glycoproteins involved in Ca2+-mediated cell-cell adhesion; see e.g., Yap et al., Annu Rev Cell Dev Biol. 1997; 13:119-46).

[0503] According to OMIM, the RET protooncogene is one of the receptor tyrosine kinases, cell-surface molecules that transduce signals for cell growth and differentiation.
The RET gene was defined as an oncogene by a classical transfection assay. RET can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement (Grieco et al., Cell 1990, 60:557-563). Mutations in the RET gene are associated with multiple endocrine neoplasia, type IIA
(MEN2A), multiple endocrine neoplasia, type IIB (MEN2B), Hirschsprung disease (HSCR; aganglionic megacolon), and medullary thyroid carcinoma (MTC) (OMIM MINI Number: 164761: 01/27/2006).

[0504] Ret (Rearranged during Transformation) was identified as a rearranged human oncogene in the classic NIH3T3 transformation assay (Takahashi et al., 1985, Cell 42(2):581-8) and subsequently characterized as a Receptor Tyrosine kinase (Takahashi et al., 1988, Oncogene 3(5):571-8).

[0505] Ret and NTRK1 (i.e., Neutrotrophic tyrosine receptor kinase 1) are receptor tyrosine kinase (RTK) proteins which play a role in the development and maturation of specific components of the nervous system. Their alterations have been associated to several human diseases, including some forms of cancer and developmental abnormalities. These features have contributed to the concept that one gene can be responsible for more than one disease. Moreover, both genes encoding for the two RTKs show genetic alterations that belong to either "gain of function" or "loss of function" class of mutations. In fact, receptor rearrangements or point mutations convert Ret and NTRK1 into dominantly acting transforming genes leading to thyroid tumors, whereas inactivating mutations, associated with Hirschsprung's disease (HSCR) and congenital insensitivity to pain with anhidrosis (CIPA), impair Ret and NTRK1 functions, respectively.

[0506] Implication of Ret in human tumorigenesis was indicated by the frequent identification of rearranged Ret sequences that transformed NIH3T3 cells in the DNA isolated from Papillary Thyroid Carcinoma DNAs. In these cases, the Ret gene was fused to as yet unknown PTC
DNA sequences in the tumor DNA but not the normal patient DNA (Grieco et al., 1990, Cell 60(4):557-63). In addition, the chromosomal mapping of Ret to chromosome 10g11.2 co-localized with genetic mapping data that implicated a gene involved in patients with MEN2A (Multiple Endocrine Neoplasia 2A) (Ishizaka et al. 1989 Oncogene 4(12):1519-21). Expression analysis of the RET oncogene in a number of human tumors consistently detected expression of normal-sized transcripts of the RET proto-oncogene in human pheochromocytomas and in human medullary thyroid carcinomas, both of familial and sporadic type (Santoro et al., 1990, Oncogene 5:1595-8).

[0507] Further analysis of the tumor DNA of patients with Multiple endocrine neoplasia type 2A
(MEN 2A) and familial medullary thyroid carcinoma (FMTC) identified mutations in the RET
sequence resulting in amino acid changes in the encoded Ret protein (Donis-Keller 1993, Hum Mol Genet. 2(7):851-6). Likewise, mutations in the RET gene were correlated with Hirschprung disease, a developmental disorder with genetic deletions and mutations in the chromosomal location of the RET
gene (Luo et al., 1993, Hum Mol Genet. 2(11):1803-8).

[0508] By early 1994, multiple papers describe the inactivation of the RET
gene in patients with Hirschsprung disease and similar phenotype in knock out mice. In addition, activating mutations in Ret are now identified in patients with MEN2A, MEN2B, and FMTC (reviewed by van Heyningen V., 1994, Nature 367(6461):319-20).

[0509] It was determined that Ret regulates cell survival. Signal transduction molecules that form a complex with Ret as a result of these phosphoryl moieties, such as GRB2, SOS, ras, and raf, propagate a signal in the cell that promotes neural survival. Thus, compounds that promote the interactions of the se stimulatory molecules of Ret would enhance the activity of c-Ret. Alternatively, protein phosphatases can remove the phosphoryl moieties placed on the intracellular region of Ret in response to GDNF, and thus inhibit the signaling capability c-Ret. Thus, compounds that inhibit phosphatases of Ret will probably enhance the signaling capacity of c-Ret.

[0510] Ret is implicated in the development and survival of enteric, synaptic, and sensory neurons and neurons of the renal system upon stimulation by GDNF (Jing, et al., 1996, Cell 85:1113-1124;
Tropp, et al., 1996, Nature 381:785-789; Durbec, et al., 1996, Nature 381:789-793). Lack of function mutations in Ret can lead to Hirschsprung's disease, for example, which manifests itself as a decrease in intestinal tract innervation in mammals. Thus, compounds that activate Ret are potential therapeutic agents for the treatment of neurodegenerative disorders, including, but not limited to, Hirschsprung's disease, Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis. Compounds that inhibit Ret function can also be anti-cancer agents as over-expression of Ret in cells is implicated in cancers, such as cancer of the thyroid.

[0511] Modulation of Ret activity may also be useful in treating cancers of the nerve tissue, such as neuroblastoma, even if an abnormality is not found the signaling pathway.

[0512] As stated above, RET gene is responsible for MEN2 syndromes, which are inherited in an autosomal dominant fashion with high penetrance and diverse clinical manifestations. The predominant RET mutation is missense mutation which is restricted to 9 codons (codons 609, 611, 618, 620, 630, 634, 768, 804 and 918). The MEN2 syndromes have 3 subtypes:
multiple endocrine neoplasia type 2A (MEN2A), MEN2B, and familial medullary thyroid carcinoma (FMTC). Missense mutations at exon 10 (codons 609, 611, 618, and 620) and exon 11 (codons 630 and 634) have been identified in 98% of MEN2A families and in 85% of FMTC families. Missense mutations at codons 768 and 804 have been known to be responsible for 5.about.10% of FMTC cases.
In addition, missense mutations at exon 16 (codon 918) have been found in 95% of MEN2B
cases.

[0513] Ret inhibitors may be useful in treating cancer of the thyroid, neuroblastoma, familial medullary thyroid carcinoma (FMTC), multiple endocrine neoplasia type IIA and IIB
(MEN2A, MEN2B), and neurodegenerative disorders (e.g. Hirschsprung's disease, Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis) [0514] Ron: Target kinase Ron (i.e., Ron protein tyrosine kinase) is a 152.2 kDa transmembrane tyrosine kinase encoded by chromosome 3p21.3 (symbol: MST1R), also known as macrophage stimulating protein receptor (i.e., MSP receptor). Ron, a member of the Met hepatocyte growth factor receptor family, was originally cloned from a human foreskin keratinocyte cDNA
library by Ronsin et al (Oncogene 1993, 8: 1195-1202). RON is expressed in various cell types including macrophages, epithelial and hematopoietic cells. Ron activation results in a variety of cellular responses in vitro, such as activation of macrophages, proliferation, migration, and invasion, which suggest a broad biologic role in vivo. Hemizygous mice (Ron +/-) grow to adulthood; however, these mice are highly susceptible to endotoxic shock and appeared to be compromised in their ability to downregulate nitric oxide production. Accordingly, Ron plays a role in early mouse development and may play a limited role in the inflammatory response. Further, Ron may be involved in cancer development and progression (OMIM MIM Number: 600168: 01/20/2006). Ron inhibitors may be useful in treating cancer and inflammation.

[0515] ROCK (ROCKI and ROCK2): Target kinase ROCK! (i.e., Rho-associated, coiled-coil containing protein kinase 1) is a 158.2 kDa serine/threonine kinase encoded by chromosome 18g11.1 (symbol: ROCK1). Target kinase ROCK2 (i.e., Rho-associated, coiled-coil containing protein kinase 2) is a 160.9 kDa serine/threonine kinase encoded by chromosome 2p24 (symbol:
ROCK2). ROCK
inhibitors may be useful in treating related to cancers (e.g. ovarian cancer, hepatocellular carcinoma, pancreatic cancer), ocular disease (e.g. glaucoma), cardiac hypertrophy, improved renal perfusion, transplant rejection, and acute respiratory distress syndrome.

[0516] Src: Target kinase Src (i.e., v-Src Avian Sarcoma Schmidt-Ruppin A-2 viral oncogene) is a 59.8 kDa non-receptor tyrosine kinase encoded by chromosome 20g12-ql3 (symbol:
SRC). The structure of Src comprises SH3 and SH2 domains adjacent to the TK domain.
According to OMIM, Azarnia et al. (Science 1988, 239:398-401) found that overexpression of the SRC gene in NIH 3T3 cells caused reduction of cell-to-cell transmission of molecules in the 400-to 700-dalton range.
Downregulation was enhanced by point mutation of tyrosine-527, whereas mutation of tyrosine-416 suppressed both the downregulation of communication by the tyr-527 mutation and that by gene overexpression. The regulation of communication by SRC may be important in the control of embryonic development and cellular growth. Luttrell et al. (Science 1999, 283:655-661) demonstrated that c-src binds to the amino terminus of beta-arrestin-1 in a complex resulting from the stimulation of beta-2 adrenergic receptors. Activated beta-2-adrenergic receptor bound beta-arrestin-1, which then bound c-src. This interaction targeted the complex to clathrin-coated pits and allowed for beta-2-adrenergic activation of the MAP kinases Erkl and Erk2. TRANCE, a TNF family member, and its receptor, RANK, are critical regulators of dendritic cell and osteoclast function.
Wong et al. (Molec. Cell 1999, 4:1041-1049) demonstrated that TRANCE activates the antiapoptotic serine/threonine kinase PKB (AKT1) through a signaling complex involving SRC
and TRAF6. A
deficiency in SRC or addition of SRC family kinase inhibitors blocked TRANCE-mediated PKB
activation in osteoclasts. SRC and TRAF6 interacted with each other and with RANK upon receptor engagement. TRAF6, in turn, enhanced the kinase activity of SRC, leading to tyrosine phosphorylation of downstream signaling molecules such as CBL. These results defined a mechanism by which TRANCE activates SRC family kinases and PKB, and provided evidence of cross-talk between TRAF proteins and SRC family kinases. Using a colon cancer cell line, Avizienyte et al. (Nature Cell Biol. 2002, 4:632-638) studied the role of SRC
in cell adhesion and metastasis. Transfection and overexpression of a constitutively active SRC
mutant reduced cell-cell contacts and caused redistribution of adherens junction components to discrete adhesion-like structures at the tips of membrane protrusions. Expression of active SRC also impaired the movement of E-cadherin from the cell interior to the plasma membrane following exposure to high calcium.
Avizienyte et al. (ibid.) provided evidence that the alpha-V and beta-1 integrins and FAK were required for the adhesion changes induced by SRC. Sandilands et al. (Dev. Cell 2004, 7:855-869) found that RhoB colocalized with active Src in the cytoplasm of mouse embryonic fibroblasts, and they presented evidence that RhoB is a component of 'outside-in' signaling pathways that coordinate Src activation with translocation to transmembrane receptors (OMIM MIM Number:
190090:
01/07/2005).

[0517] The Src family of cytoplasmic protein tyrosine kinases consists of at least eight members (Src, Fyn, Lyn, Yes, Lek, Fgr, Hck and Blk) that participate in a variety of signaling pathways [Schwartzberg, P. L., Oncogene, 17, 1463 (1998)]. The prototypical member of this tyrosine kinase family is p60src (Src). Src is involved in proliferation and migration responses in many cell types. In limited studies, Src activity has been shown to be elevated in breast, colon (-90%), pancreatic (>90%) and liver (>90%) tumors. Greatly increased Src activity is also associated with metastasis (>90%) and poor prognosis. Antisense Src message impedes growth of colon tumor cells in nude mice [Staley et al., Cell Growth & Differentation, 8, 269 (1997)], suggesting that Src inhibitors should slow tumor growth. In addition to its role in cell proliferation, Src also acts in stress response pathways, including the hypoxia response. Previous studies have shown that colonic tumor cells genetically engineered to express antisense Src message form tumors demonstrating reduced vascularization in nude mouse models [Ellis, et al., J. Biol. Chem., 273, 1052 (1998)], suggesting that Src inhibitors would be anti-angiogenic as well as anti-proliferative.

[0518] Apart from its role in cancer, Src also appears to play a role in osteoporosis. Mice genetically engineered to be deficient in src production were found to exhibit osteopetrosis, the failure to resorb bone [Soriano, P., Cell, 64, 693 (1991); Boyce, B. F., J. Clin. Invest., 90, 1622 (1992)]. This defect was characterized by a lack of osteoclast activity. Since osteoclasts normally express high levels of Src, inhibition of Src kinase activity may be useful in the treatment of osteoporosis [Missbach, M., Bone, 24, 437 (1999)]. Src inhibitors may be useful in treating cancer and osteoporosis.

[0519] Stk6: Target Stk6 (i.e., Serine/Threonine protein kinase 6) is a 45.8 kDa serine(threonine kinase encoded by chromosome 20g13.2-gl3.3 (symbol: STK6). According to OMIM, Kimura et al.
(J. Biol. Chem. 1997, 272:13766-13771) cloned a eDNA encoding a novel human serine/threonine kinase, STK6, that has high homology with the Aurora and Ipll kinases.
Mutations in these yeast kinases are known to cause abnormal spindle formation and missegregation of chromosomes.
Northern and Western blot analyses revealed a high level of STK6 expression product in testis and proliferating culture cells such as HeLa cells. The endogenous levels of STK6 protein and protein kinase activity were tightly regulated during cell cycle progression in HeLa cells. T he protein was upregulated during G2/M and rapidly reduced after mitosis. Immunofluorescence studies revealed specific localization of STK6 protein to the spindle pole region during mitosis. The results suggested that STK6, like Aurora and Ipll, is involved in cell growth and/or chromosome segregation (OMIM
MIM Number: 602687: 04/01/2003).

[0520] Aurora A belongs to the family of STKs that are involved in mitotic events such as centrosome separation and chromosome segregation and are therefore essential for cell proliferation (Bischoff & Plowman, Trends Cell Biol. 1999, 9:454459); Giet & Prigent, 1999, Cell Science 112:
3591-3601). Inhibitors of the Aurora kinase family therefore have the potential to block growth of all tumors.

[0521] The three identified Aurora kinases of this family are known under various names: Aurora-A
(Aurora 1), Aurora B (Aurora 2) and Aurora C (Aurora 3). Alternate names for Aurora A, described here further are serine/threonine protein kinase 15 (STK 15), BTAK Aurora-related kinase 1 (ARK1).
The mouse homolog of Aurora A, STK6 also referred to as AIK, is highly homologous to STK15. All Aurora kinase family members are highly homologous proteins responsible for mitotic events such as centrosome maturation and seggregation, chromosome segregation, mitotic spindle function and cytokinesis. Peak expression of Aurora occurs during the G2 and mitotic phase in cycling cells and then decreases and remains low or undetectable in resting cells (Shindo et al., 1998, Bioc/aem.
Biophys. Res. Commun. 244: 285-292). In mammalian cells proposed substrates for Aurora include histone 113, a protein involved in chromosome condensation, and CENP-A, myosin 11 regulatory light chain, protein phosphatase 1, TPX2, all of which are required for cell devision.

[0522] Zhou et al., (Zhou et al., 1998, Nature Genet. 20: 189-193) found that Aurora is involved in the induction of centrosome duplication-distribution abnormalities and aneuploidy in mammalian cells. Centrosomes appear to maintain genomic stability through the establishment of bipolar spindles during cell division, ensuring equal segregation of replicated chromosomes to 2 daughter cells.
Deregulated duplication and distribution of centrosomes are implicated in chromosome segregation abnormalities, leading to aneuploidy seen in many cancer cell types. Zhou et al., (Zhou et al., 1998, Nature Genet. 20: 189-193) found amplification of Aurora A in approximately 12% of primary breast tumors, as well as in breast, ovarian, colon, prostate, neuroblastoma, and cervical cancer cell lines.
Additionally, high expression of Aurora A mRNA was detected in tumor cell lines without evidence of gene amplification. Ectopic expression of Aurora A in mouse NIH 3T3 cells led to the appearance of abnormal centrosome number (amplification) and transformation in vitro.
Finally, over-expression of Aurora A in near-diploid human breast epithelial cells revealed similar centrosome abnormality, as well as induction of aneuploidy. These findings suggested that Aurora A is a critical kinase-encoding gene, whose over-expression leads to centrosome amplification, chromosomal instability, and transformation in mammalian cells.

[0523) The AURKA gene is over expressed in many human cancers. Ectopic over-expression of aurora kinase A in mammalian cells induces centrosome amplification, chromosome instability, and oncogenic transformation, a phenotype characteristic of loss-of-function mutations of p53. Katayama et al. (Katayama et al., 2004, Nature Genet. 36: 55-62) showed that Aurora A
kinase phosphorylates p53 at ser315, leading to its ubiquitination by MDM2 and proteolysis. P53 is not degraded in the presence of inactive Aurora A or ubiquitination-defective MDM2. Silencing of Aurora kinase A
results in less phosphorylation of p53 at ser315, greater stability of p53, and cell-cycle arrest at G2-M.
Cells depleted of Aurora kinase A are more sensitive to cisplatin-induced apoptosis, and elevated expression of aurora kinase A abolishes this response. In a sample of bladder tumors with wildtype p53, (Katayama et al., 2004, Nature Genet. 36: 55-62) found a correlation between elevated expression of aurora kinase A and low p53 concentration. They concluded that Aurora A kinase is a key regulatory component of the p53 pathway and that over-expression of Aurora A leads to increased degradation of p53, causing down-regulation of checkpoint-response pathways and facilitating oncogenic transformation of cells.

[0524) By immunoprecipitation of epitope-tagged proteins from transfected HEK293 cells, Kunitoku et al. (Kunitoku et al., 2003, Dev. Cell 5: 853-854) demonstrated direct interaction between the genes CENPA and AURKA. In vitro, AURKA phosphorylated CENPA on ser7, a residue that is also phosphorylated by AURKB. Examination of the role of both kinases in the phosphorylation of CENPA revealed that the reaction is mediated sequentially by AURKA and AURKB
in early mitosis.
Mitotic cells in which phosphorylation of CENPA on ser7 was prevented exhibited a substantial proportion of misaligned chromosomes resulting from a defect in the ability of kinetochores to attach to microtubules.

[0525) By yeast 2-hybrid analysis of HeLa cells, Hirota et al. (Hirota et al., 2003, Cell 114: 585-598) determined that AURKA interacts with Ajuba (J JB). The two proteins interacted in mitotic cells and became phosphorylated as they did so. In vitro analysis revealed that Ajuba induced the autophosphorylation and consequent activation of AURKA. Depletion of Ajuba prevented activation of AURKA at centrosomes in late G2 phase and inhibited mitotic entry. Hirota et al. (Hirota et al., 2003, Cell 114: 585-598) concluded that Ajuba is an essential activator of AURKA in mitotic commitment.

[0526] The mammalian Aurora kinase family has been implicated in tumorigenesis of a variety of different cancers. The main role of Aurora A in tumor development is in controlling chromosome segregation during mitosis (Bischoff & Plowman, Trends Cell Biol. 1999, 9:454-459). Over-expression of Aurora A transforms rodent fibroblasts (Bischoff et al., 1998, EMBO J. 17:3052-3065).
The elevated levels of Aurora A induce misregulation of chromosome segregation that results in cells containing multiple centrosomes and multipolar spindles leading to aneuploidy, a distinctive feature of most cancers. (thou et al., 1998, Nature Genet. 20: 189-193). Ewart-Toland et al. (Ewart-Toland et al., 2003, Nature Genet. 34: 403-412) found that tumors from individuals carrying the 91A allele showed more evidence of aneuploidy than those from individuals who were homozygous for the common 91T allele. They concluded that individuals with even one copy of the Aurora A 91A allele develop tumors that have on average a higher degree of aneuploidy than those from individuals homozygous for 91T. The oncogenic activity of Aurora kinases is likely to be linked to the generation of such genetic instability. Miyoshi et al. (Miyoshi et al., 2001, Int. J.
Cancer 92: 370-373) and Sakakura et al. (Sakakura, et al., 2001, Br. J. Cancer 84: 824-831) report a correlation between amplification of the Aurora A locus and chromosomal instability in mammary and gastric tumors.
[0527] Over-expression of Aurora kinases have been reported in a wide range of human tumors.
Aurora A expression is elevated in tumor cell lines derived from colon, breast, lung, melanoma, kidney, ovary, pancreas, CNS, gastric tract and leukemia cells (Tatsuka et al., 1998, Cancer Res.
58(21): 4811-6.) Elevated expression of Aurora A has been detected in over 50%
of colorectal, ovarian and gastric tumors and in 94% of invasive duct adenocarcinomas of the breast (Colorectal tumors: Bischoff et al., 1998, EMBO J. 17:3052-3065, Takahashi et al., 2000, Jpn. J. Cancer Res.
91:1007-1014, Ovarian tumors: Gritsko et al., 2003, Clin. Cancer Res. 9:1420-1426; gastric tumors:
Sakakura, et al., 2001, Br. J. Cancer 84: 824-831; breast tumors: Tanaka et al., 1999, Cancer Res.
59:2041-2044). High levels of Aurora A have also been reported in renal, cervical, neuroblastoma, melanoma, lymphoma, pancreatic and prostate tumor cell lines (Bischoff et al., 1998, EMBO J.
17:3052-3065; Zhou et al., 1998, Nature Genetics 20:189-193; Li et al., 2003, Clin. Cancer Res. 9(3):
991-997). Amplification and over-expression of Aurora A is further observed in human bladder cancers and where it is associated with aneuploidy and aggressive clinical behavior (Sen et al., 2002, J. Natl. Cancer Inst. 94(17): 1320-1329). Isola et al. (Isola et al., 1995, Am. J. Pathology 147: 905-911) further found that amplification of the Aurora A locus correlates with poor prognosis for patients with node-negative breast cancer.

[0528] Based on the known function of the Aurora kinases, inhibition of their activity should disrupt mitosis leading to cell cycle arrest. In vivo, an Aurora inhibitor therefore slows tumor growth and induces regression. Stk6 inhibitors may be useful in treating gastric, bladder, breast, lung, CNS, ovarian, kidney, colon, prostate, pancreas, and cervical cancers, melanoma, leukemia, and neuroblastoma.

[0529] Syk: Target kinase Syk (i.e., spleen tyrosine kinase) is a 72.1 kDa tyrosine kinase encoded by chromosome 9q22.2 (symbol: SYK). Syk inhibitors may be useful in treating lymphomas, such as mantle cell lymphoma.

[0530] TEC: Target kinase TEC (i.e., tec protein tyrosine kinase) is a 73.6 kDa non receptor tyrosine kinase encoded by chromosome 4p12 (symbol: TEC). TEC inhibitors may be useful in treating sepsis, septic shock, inflammation, rheumatoid arthritis, Crohn's disease, irritable bowel disease (IBD), and ulcerative colitis.

[0531] Tie2: Target kinase Tie2 (i.e., tyrosine kinase, endothelial) is a 125.8 kDa receptor tyrosine kinase encoded by chromosome 9p21 (symbol: TEK). Tie2 inhibitors may be useful in treating cancer, arthritis (e.g. rheumatoid arthritis), and atherosclerosis.

[0532] TrkA: Target kinase TrkA (i.e., neurotrophic tyrosine kinase, receptor, type 1) is a 87.5 kDa tyrosine kinase encoded by chromosome 1 q21-q22 (symbol: NTRK1). TrkA
inhibitors may be useful in treating pain (e.g. chronic pain, neuropathic pain), cancer, arthritis, diabetic retinopathy, macular degeneration and psoriasis.

[0533] Yes: Target kinase Yes (i.e., Yamaguchi Sarcoma Oncogene homolog 1) is a 60.8 kDa tyrosine kinase encoded by chromosome 18p 11.31-p 11.21 (symbol: YES 1). The structure of Yes comprises SH3 and SH2 domains followed by a TK domain. The YES oncogene is homologous to the Yamaguchi sarcoma virus gene, and the amino acid sequence of Yes shows a high degree of homology with that of the SRC gene product of Rous sarcoma virus. The Yes kinase is highly expressed in multiple mammalian cell types, including neurons, spermatozoa, platelets, and epithelial cells. The target kinase Yes is amplified and overexpressed in various cancers including esophageal squamous cell carcinoma. Yes inhibitors may be useful in treating cancers including esophageal squamous cell carcinoma.

[0534] Zap70: Target kinase Zap70 (i.e., Zeta-chain associated protein kinase, 70 kDa) is a 69.9 kDa tyrosine kinase encoded by chromosome 2g11-13 (symbol: ZAP70). Zap70 was first reported by Chan et al. (Cell 1992, 71: 649-662). The mature protein comprises two SH2 domains and a TK
domain.

[0535] Zap70 is crucial to the transduction of the T-cell receptor (TCR) signalling pathway, which leads ultimately to cellular differentiation and proliferation. (Weiss &
Imboden 1987, Adv. Immunol.
41: 1-38). On stimulation of the T-cell antigen receptor tyrosine phosphorylation takes place in a number of intracellular substrates, mediated by sequential activation of two distinct families of cytoplasmic PTKs. One substrate is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions. The Src kinases Lck and Fyn phosphorylate tyrosine residues on the TCR zeta-chain, contained within conserved sequences known as immunoreceptor tyrosine-based activation motifs (ITAMs). The Zap70 protein associates with the phosphorylated ITAMs in the zeta chain of the activated TCR complex (Chan et al,, 1991, PNAS 88:
9166-9170. Recruitment of Zap70 to the TCR and its subsequent phosphorylation and activation triggers all downstream signaling events (Irving & Weiss, 1991, Cell 64: 891-902). This interaction is believed to be critical for TCR signaling, since zeta-phosphopeptides that block the interaction of Zap70 with the zeta-chain also inhibit TCR signaling events (Waage et al., 1995, J. Biol. Chem.
270:944-948).

[05361 The essential role of Zap70 in T-cell function has been demonstrated in human patients, human T-cell lines and mice. Elder et al. (Elder et al., 1997, J. of Pedriatic Hematology/Oncology 19(6): 546-550) reported studies of human patient suffering from a rare form of severe combined immune deficiency syndrome (SCID). The patient was found to be homozygous for a 13-bp deletion involving nucleotides 1719-1731 of the ZAP70 gene, resulting in premature termination 35 codons downstream and yielding a mutant protein 82 residues shorter than wildtype Zap70 . This kind of patients have profound immunodeficiency, lack CD8+ T-cells and have CD4+ T-Cells that are unresponsive to T-cell receptor (TCR)-mediated stimulation. Following TCR
activation the CD4+
cells show severe defects in Ca2+ mobilization, tyrosine phosphorylation of down-stream substrates, proliferation and IL-2 production (Elder et al., Pedriatric Research 39: 743-748).

[0537] ZAP70 deficient human Jurkat cells also demonstrate the important function of ZAP70 in T-cell receptor signaling. A Jurkat clone (p116) lacking the Zap70 protein was shown to have defects in TCR signaling which was correctable by re-introduction of wild type ZAP70 (Williams et al., 1998, Molecular and Cellular Biology 18 (3): 1388-1399.) [05381 Studies of ZAP70 deficient mice also underline the crucial role of the PTK in T-cell signal transduction. ZAP70 deficient mice had neither CD4 nor CD8 single-positive T
cells, but human Zap70 reconstitutes both CD4 and CD8 single-positive populations. Besides the defects in T-cell development the TCR signalling in thymocytes was found to be profoundly impaired, suggesting that Zap70 is a central signalling molecule during thymic selection for CD4 and CD8 lineage. (Negishi et al.,1995, Nature 376: 435-438, Sakaguchi et al. (Sakaguchi et al. 2003, Nature 426: 454-460) reported that the mouse strain SKG, which is derived from a closed breeding colony of BALB/c mice, spontaneously develops chronic arthritis. This autosomal recessive trait was found to be caused by a mutation (W163C) in the second SH2 domain of Zap70. The phenotype showed altered signal transduction from TCRs and a change in the threshold of T-cells to thymic selection, leading to the positive selection of otherwise negatively selected autoimmune T-cells.

[0539] The importance of the Zap70 kinase domain function has been demonstrated by Elder et al.
(Elder et al,, 2001, J. Immunology 166(1): 656-661). In studies of humans patients and mice showed that missense mutations within the highly conserved DLAARN motif within the kinase domain of Zap70 result in SCID. This mutation caused the loss of catalytic function of Zap70, resulting in defective T-cell receptor signaling. The requirement of the catalytic function of Zap70 was further illustrated by Williams et al., who found an inactive Zap7O mutant (Lys369Arg) was unable to restore TCR signaling in a ZAP70 deficient Jurkat cell clone (p116) (Williams et al., 1998, Mol. Cell Biology 18 (3): 1388-1399).

[0540] Zap70 further participates in early B-cell differentiation and is a prognostic factor in chronic lymphocytic leukaemia (CLL). The course of CLL is variable. Crespo and coworkers (Crespo et al., 2003, New Eng. J. Med. 348: 1764-1775) found that Zap70 expression by cells in CLL is a simple and reliable surrogate for the identification of immunoglobulin heavy chain variable region mutations. In the aggressive progression of the disease, Zap70 is associated with CLL cells expressing an unmutated configuration of the immunoglobulin heavy chain variable region gene (IgVH) (Carreras et al., 2005, Journal of Pathology 205 (4): 507-513). Whereas in indolent disease, the CLL cells usually express a mutated immunoglobulin heavy chain variable region gene but lack expression of ZAP70.
Rassenti et al. (Rassenti et al., 2004, New Eng. J. Med. 351: 893-901) found that although the presence of an unmutated immunoglobulin heavy chain variable region gene in CLL patients was strongly associated with expression of Zap70, Zap70 was a stronger predictor of the need for treatment in B-cell CLL. T-cell Zap70 overexpression in CLL patients was found to not only correlate with Zap70levels in CLL cells, but also with clinical stage and disease progression. (Herishanu et al., 2005, Leukemia advance online publication).

[0541] The protein tyrosine kinase Zap70 functions in the signaling pathway that plays an essential role in T-cell activation and development. After TCR stimulation, Zap70 is associated with the receptor complex through the interaction of its two SH2 domains with the doubly phosphorylated ITAMs. The association of Zap70 with the TCR ITAMs facilitates its autophosphorylation and the tyrosine phosphorylation of Zap70 mediated by Src family PTKs, e.g. Lck and Fyn. (Iwashima et al., 1994, Science 263:1136-1139). The recruitment of adaptor molecules like Lat and S1p76 to Zap70 in turn couple to more distal signaling pathways including Ras and PLC-gamma (Chu et al., 2003, Journal of Biology 2 (3): 2-21).

[05421 Mutations in the ZAP70 gene are associated with the selective T-cell defect (STD) and severe combined immunodeficiency (SCID) in human patients. The variety of point mutations, missense mutations, deletions and chromosomal rearrangements are identified in Zap70 all result in the same phenotype (OMIM database with genetic mutations).

[0543] Zap70 inhibitors may be useful in treating autoimmune, inflammatory, proliferative and hyperproliferative diseases, immunologically mediated diseases, AIDS, systemic lupus erythematosus, myasthenia gravis, atherosclerosis, rejection of transplanted organs or tissues, allograft rejection including acute and chronic allograft rejection, graft versus host disease, rheumathoid arthritis, psoriasis, systemic sclerosis, atopic dermatitis, eczematous dermatitis, alopecia, and inflammation of the nasal mucus membrane, including all forms of rhinitis.

III. Binding Assays [0544] The methods of the present invention can involve assays that are able to detect the binding of compounds to a target molecule. Such binding is at a statistically significant level, preferably with a confidence level of at least 90%, more preferably at least 95, 97, 98, 99% or greater confidence level that the assay signal represents binding to the target molecule, i.e., is distinguished from background.
Preferably controls are used to distinguish target binding from non-specific binding. A large variety of binding assays are known for different target types and can be used for this invention.

[0545] Binding compounds can also be characterized by their effect on the activity of the target molecule. Compounds of the present invention (i.e., compounds of Formula I, including Formulae la - Iz, and all sub-embodiments disclosed herein, or Formula II, including Formulae Ila - Ho, and all sub-embodiments disclosed herein) may be assayed with respect to a particular kinase to assess inhibitory concentration (IC50) or excitation concentration (EC50) of the compound with respect to that kinase. The IC50 (or EC50) is defined as the concentration of compound at which 50% of the activity of the target kinase activity being measured is lost (or gained) relative to activity when no compound is present. Activity can be measured using methods known to those of ordinary skill in the art, e.g., by measuring any detectable product or signal produced by occurrence of an enzymatic reaction, or other activity by a protein being measured. Compounds will have an IC50 or EC50 of less than 10 M, also less than 1 M, also less than 100 nM, also less than 10 nM or less than 1 nM.

[0546] By "background signal" in reference to a binding assay is meant the signal that is recorded under standard conditions for the particular assay in the absence of a test compound, molecular scaffold, or ligand that binds to the target molecule. Persons of ordinary skill in the art will realize that accepted methods exist and are widely available for determining background signal.

[0547] By "standard deviation" is meant the square root of the variance. The variance is a measure of how spread out a distribution is. It is computed as the average squared deviation of each number from its mean, For example, for the numbers 1, 2, and 3, the mean is 2 and the variance is:
6Z = L1-2L+ (2-2)z + (3-2) z = 0.667 .

Surface Plasmon Resonance [0548] Binding parameters can be measured using surface plasmon resonance, for example, with a BlAcore chip (Biacore, Japan) coated with immobilized binding components.
Surface plasmon resonance is used to characterize the microscopic association and dissociation constants of reaction between an sFv or other ligand directed against target molecules. Such methods are generally described in the following references. Vely F. et al., (2000) BlAcore analysis to test phosphopeptide-SH2 domain interactions, Methods in Molecular Biology. 121:313-21; Liparoto et al., (1999) Biosensor analysis of the interleukin-2 receptor complex, Journal of Molecular Recognition. 12:316-21; Lipschultz et al., (2000) Experimental design for analysis of complex kinetics using surface plasmon resonance, Methods.
20(3):310-8; Malmqvist., (1999) BIACORE: an affinity biosensor system for characterization of biomolecular interactions, Biochemical Society Transactions 27:335-40; Alfthan, (1998) Surface plasmon resonance biosensors as a tool in antibody engineering, Biosensors & Bioelectronics. 13:653-63;
Fivash et al., (1998) BlAcore for macromolecular interaction, Current Opinion in Biotechnology. 9:97-101; Price et al.;
(1998) Summary report on the ISOBM TD-4 Workshop: analysis of 56 monoclonal antibodies against the MUC1 mucin. Tumour Biology 19 Suppl 1:1-20; Malmqvist et al, (1997) Biomolecular interaction analysis: affinity biosensor technologies for functional analysis of proteins, Current Opinion in Chemical Biology. 1:378-83; O'Shannessy et al., (1996) Interpretation of deviations from pseudo-first-order kinetic behavior in the characterization of ligand binding by biosensor technology, Analytical Biochemistry. 236:275-83; Malmborg et al,, (1995) BlAcore as a tool in antibody engineering, Journal of Immunological Methods. 183:7-13; Van Regenmortel, (1994) Use of biosensors to characterize recombinant proteins, Developments in Biological Standardization.
83:143-51; and O'Shannessy, (1994) Determination of kinetic rate and equilibrium binding constants for macromolecular interactions: a critique of the surface plasmon resonance literature, Current Opinions in Biotechnology. 5:65-71.

[0549] BlAcore uses the optical properties of surface plasmon resonance (SPR) to detect alterations in protein concentration bound to a dextran matrix lying on the surface of a gold/glass sensor chip interface, a dextran biosensor matrix. In brief, proteins are covalently bound to the dextran matrix at a known concentration and a ligand for the protein is injected through the dextran matrix. Near infrared light, directed onto the opposite side of the sensor chip surface is reflected and also induces an evanescent wave in the gold film, which in turn, causes an intensity dip in the reflected light at a particular angle known as the resonance angle. If the refractive index of the sensor chip surface is altered (e.g., by ligand binding to the bound protein) a shift occurs in the resonance angle. This angle shift can be measured and is expressed as resonance units (RUs) such that 1000 RUs is equivalent to a change in surface protein concentration of 1 ng/mm2. These changes are displayed with respect to time along the y-axis of a sensorgram, which depicts the association and dissociation of any biological reaction.

High Throughput Screening (HTS) Assays [0550] HTS typically uses automated assays to search through large numbers of compounds for a desired activity. Typically HTS assays are used to find new drugs by screening for chemicals that act on a particular enzyme or molecule. For example, if a chemical inactivates an enzyme it might prove to be effective in preventing a process in a cell which causes a disease. High throughput methods enable researchers to assay thousands of different chemicals against each target molecule very quickly using robotic handling systems and automated analysis of results.

[0551] As used herein, "high throughput screening" or "HTS" refers to the rapid in vitro screening of large numbers of compounds (libraries); generally tens to hundreds of thousands of compounds, using robotic screening assays. Ultra high-throughput Screening (uHTS) generally refers to the high-throughput screening accelerated to greater than 100,000 tests per day.

[0552] To achieve high-throughput screening, it is advantageous to house samples on a multicontainer carrier or platform. A multicontainer carrier facilitates measuring reactions of a plurality of candidate compounds simultaneously. Multi-well microplates may be used as the carrier.
Such multi-well microplates, and methods for their use in numerous assays, are both known in the art and commercially available.

[0553] Screening assays may include controls for purposes of calibration and confirmation of proper manipulation of the components of the assay. Blank wells that contain all of the reactants but no member of the chemical library are usually included. As another example, a known inhibitor (or activator) of an enzyme for which modulators are sought, can be incubated with one sample of the assay, and the resulting decrease (or increase) in the enzyme activity used as a comparator or control.
It will be appreciated that modulators can also be combined with the enzyme activators or inhibitors to find modulators which inhibit the enzyme activation or repression that is otherwise caused by the presence of the known the enzyme modulator. Similarly, when ligands to a sphingolipid target are sought, known ligands of the target can be present in control/calibration assay wells.

Measuring Enzymatic and Binding Reactions During Screening Assays [0554] Techniques for measuring the progression of enzymatic and binding reactions, e.g., in multicontainer carriers, are known in the art and include, but are not limited to, the following.

[0555) Spectrophotometric and spectrofluorometric assays are well known in the art. Examples of such assays include the use of colorimetric assays for the detection of peroxides, as described in Gordon, A. J. and Ford, R. A., (1972) The Chemist's Companion: A Handbook Of Practical Data, Techniques, And References, John Wiley and Sons, N.Y., Page 437.

[0556] Fluorescence spectrometry may be used to monitor the generation of reaction products.
Fluorescence methodology is generally more sensitive than the absorption methodology. The use of fluorescent probes is well known to those skilled in the art. For reviews, see Bashford et al., (1987) Spectrophotometry and Spectrofluorometry: A Practical Approach, pp. 91-114, IRL Press Ltd.; and Bell, (1981) Spectroscopy In Biochemistry, Vol. I, pp. 155-194, CRC Press.

[0557) In spectrofluorometric methods, enzymes are exposed to substrates that change their intrinsic fluorescence when processed by the target enzyme. Typically, the substrate is nonfluorescent and is converted to a fluorophore through one or more reactions. As a non-limiting example, SMase activity can be detected using the Amplex Red reagent (Molecular Probes, Eugene, OR).
In order to measure sphingomyelinase activity using Amplex Red, the following reactions occur.
First, SMase hydrolyzes sphingomyelin to yield ceramide and phosphorylcholine. Second, alkaline phosphatase hydrolyzes phosphorylcholine to yield choline. Third, choline is oxidized by choline oxidase to betaine. Finally, H202, in the presence of horseradish peroxidase, reacts with Amplex Red to produce the fluorescent product, Resorufin, and the signal therefrom is detected using spectrofluorometry.

[0558] Fluorescence polarization (FP) is based on a decrease in the speed of molecular rotation of a fluorophore that occurs upon binding to a larger molecule, such as a receptor protein, allowing for polarized fluorescent emission by the bound ligand. FP is empirically determined by measuring the vertical and horizontal components of fluorophore emission following excitation with plane polarized light. Polarized emission is increased when the molecular rotation of a fluorophore is reduced. A
fluorophore produces a larger polarized signal when it is bound to a larger molecule (i.e. a receptor), slowing molecular rotation of the fluorophore. The magnitude of the polarized signal relates quantitatively to the extent of fluorescent ligand binding. Accordingly, polarization of the "bound"
signal depends on maintenance of high affinity binding.

[0559] FP is a homogeneous technology and reactions are very rapid, taking seconds to minutes to reach equilibrium. The reagents are stable, and large batches may be prepared, resulting in high reproducibility. Because of these properties, FP has proven to be highly automatable, often performed with a single incubation with a single, premixed, tracer-receptor reagent. For a review, see Owickiet al., (1997), Application of Fluorescence Polarization Assays in High-Throughput Screening, Genetic Engineering News, 17:27.

[0560] FP is particularly desirable since its readout is independent of the emission intensity (Checovich, W. J., et al., (1995) Nature 375:254-256; Dandliker, W. B., et al., (1981) Methods in Enzymology 74:3-28) and is thus insensitive to the presence of colored compounds that quench fluorescence emission. FP and FRET (see below) are well-suited for identifying compounds that block interactions between sphingolipid receptors and their ligands. See, for example, Parker et al., (2000) Development of high throughput screening assays using fluorescence polarization: nuclear receptor-ligand-binding and kinase/phosphatase assays, J Biomol Screen 5:77-88.

[0561] Fluorophores derived from sphingolipids that may be used in FP assays are commercially available. For example, Molecular Probes (Eugene, OR) currently sells sphingomyelin and one ceramide flurophores. These are, respectively, N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene- 3-pentanoyl)sphingosyl phosphocholine (BODIPY FL C5-sphingomyelin);
N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene- 3-dodecanoyl)sphingosyl phosphocholine (BODIPY FL C12-sphingomyelin); and N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene- 3-pentanoyl)sphingosine (BODIPY FL CS-ceramide). U.S. Patent No.
4,150,949, (Immunoassay for gentamicin), discloses fluorescein-labelled gentamicins, including fluoresceinthiocarbanyl gentamicin. Additional fluorophores may be prepared using methods well known to the skilled artisan.

[0562] Exemplary normal-and-polarized fluorescence readers include the POLARION
fluorescence polarization system (Tecan AG, Hombrechtikon, Switzerland).
General multiwell plate readers for other assays are available, such as the VERSAMAX reader and the SPECTRAMAX
multiwell plate spectrophotometer (both from Molecular Devices).

[0563] Fluorescence resonance energy transfer (FRET) is another useful assay for detecting interaction and has been described. See, e.g., Heim et al., (1996) Curr. Biol.
6:178-182; Mitra et al., (1996) Gene 173:13-17; and Selvin et al., (1995) Meth. Enzymol. 246:300-345.
FRET detects the transfer of energy between two fluorescent substances in close proximity, having known excitation and emission wavelengths. As an example, a protein can be expressed as a fusion protein with green fluorescent protein (GFP). When two fluorescent proteins are in proximity, such as when a protein specifically interacts with a target molecule, the resonance energy can be transferred from one excited molecule to the other. As a result, the emission spectrum of the sample shifts, which can be measured by a fluorometer, such as a fMAX multiwell fluorometer (Molecular Devices, Sunnyvale Calif.).

[0564] Scintillation proximity assay (SPA) is a particularly useful assay for detecting an interaction with the target molecule. SPA is widely used in the pharmaceutical industry and has been described (Hanselman et al., (1997) J. Lipid Res. 38:2365-2373; Kahl et al., (1996) Anal. Biochem. 243:282-283; Undenfriend et al., (1987) Anal. Biochem. 161:494-500). See also U.S.
Patent Nos. 4,626,513 and 4,568,649, and European Patent No. 0,154,734. One commercially available system uses FLASHPLATE scintillant-coated plates (NEN Life Science Products, Boston, MA).

[05651 The target molecule can be bound to the scintillator plates by a variety of well known means.
Scintillant plates are available that are derivatized to bind to fusion proteins such as GST, His6 or Flag fusion proteins. Where the target molecule is a protein complex or a multimer, one protein or subunit can be attached to the plate first, then the other components of the complex added later under binding conditions, resulting in a bound complex.

[05661 In a typical SPA assay, the gene products in the expression pool will have been radiolabeled and added to the wells, and allowed to interact with the solid phase, which is the immobilized target molecule and scintillant coating in the wells. The assay can be measured immediately or allowed to reach equilibrium. Either way, when a radiolabel becomes sufficiently close to the scintillant coating, it produces a signal detectable by a device such as a TOPCOUNT NXT microplate scintillation counter (Packard BioScience Co., Meriden Conn.). If a radiolabeled expression product binds to the target molecule, the radiolabel remains in proximity to the scintillant long enough to produce a detectable signal.

[05671 In contrast, the labeled proteins that do not bind to the target molecule, or bind only briefly, will not remain near the scintillant long enough to produce a signal above background. Any time spent near the scintillant caused by random Brownian motion will also not result in a significant amount of signal. Likewise, residual unincorporated radiolabel used during the expression step may be present, but will not generate significant signal because it will be in solution rather than interacting with the target molecule. These non-binding interactions will therefore cause a certain level of background signal that can be mathematically removed. If too many signals are obtained, salt or other modifiers can be added directly to the assay plates until the desired specificity is obtained (Nichols et al., (1998) Anal. Biochem. 257:112-119).

1V. Kinase Activity Assays [0568] A number of different assays for kinase activity can be utilized for assaying for active modulators and/or determining specificity of a modulator for a particular kinase or group or kinases.
In addition to the assay mentioned in the Examples below, one of ordinary skill in the art will know of other assays that can be utilized and can modify an assay for a particular application. For example, numerous papers concerning kinases describe assays that can be used.

[0569] Additional alternative assays can employ binding determinations. For example, this sort of assay can be formatted either in a fluorescence resonance energy transfer (FRET) format, or using an AlphaScreen (amplified luminescent proximity homogeneous assay) format by varying the donor and acceptor reagents that are attached to streptavidin or the phosphor-specific antibody.

V. Organic Synthetic Techniques [0570] The versatility of computer-based modulator design and identification lies in the diversity of structures screened by the computer programs. The computer programs can search databases that contain very large numbers of molecules and can modify modulators already complexed with the enzyme with a wide variety of chemical functional groups. A consequence of this chemical diversity is that a potential modulator of kinase function may take a chemical form that is not predictable. A
wide array of organic synthetic techniques exist in the art to meet the challenge of constructing these potential modulators. Many of these organic synthetic methods are described in detail in standard reference sources utilized by those skilled in the art. One example of suh a reference is March, 1994, Advanced Organic Chemistr , Reactions, Mechanisms and Structure, New York, McGraw Hill.
Thus, the techniques useful to synthesize a potential modulator of kinase function identified by computer-based methods are readily available to those skilled in the art of organic chemical synthesis.
[0571] Regarding the synthetic examples described herein, solvents include polar and non-polar solvents known to those of skill in the art, including polar aprotic and polar protic solvents. Polar solvents include, without limitation, protic solvents such as methanol, ethanol, isopropyl alcohol, t-butanol, n-butanol, acetic acid, formic acid or water, or aprotic solvents such as tetrahydrofuran (THF), acetonitrile, dioxane, methylene chloride, dimethylsulfoxide (DMSO), acetone, N,N-dimethylformamide (DMF), N,N-dimethylacetamide (DMA), ethyl acetate, 1,2-dimethoxyethane, 1,2-dichloroethane, chloroform, 1,2-dichloroethane, or pyridine. Polar solvents include a mixture of water with any of the above, or a mixture of any two or more of the above. Apolar solvents include, without limitation, toluene, benzene, chlorobenzene, xylenes and hexanes.

[0572] Regarding the synthetic examples described herein, reducing agent includes, without limitation, a reducing agent such as catalytic reducing agents using hydrogen and transition metal catalysts such as palladium, platinum, rhodium, etc.(e.g. Pt/acetic acid/H2); a mixture of trifluoroacetic acid and triethylsilane, borane tetrahydrofuran complex, diborane, borane dimethylsulfide complex, and a combination of sodium borohydride and boron trifluoride;
metals such as reduced iron, zinc powder, magnesium etc.; metal hydrogen complex compounds such as alkali metal borohydrides (for example, potassium borohydride, sodium borohydride, lithium borohydride, zinc borohydride, sodium triacetoxyborohydride, etc.), aluminum lithium hydride, etc.; metal hydrides such as sodium hydride, etc.;
organic tin compounds (triphenyltin hydride, etc.); and metal salts such as nickel compounds, zinc compounds, tin compounds (for example tin(II) chloride), and samarium iodide/pivalic acid/hexamethylphorphoric triamide.

[0573] Regarding the synthetic examples described herein, oxidizing agent includes, without limitation, an oxidizing agent such as Dess-Martin reagent, TEMPO
(2,2,6,6-tetramethylpiperidine-N-oxide), DDQ (2,3-Dichloro-5,6-dicyano-1,4-benzoquinone), PDC
(pyridinium dichromate), PCC (pyridinium chlorochromate), Pyridine.S03, Chromium trioxide, p-nitroperbenzoic acid, magnesium monoperoxyphthalate, sodium periodate, potassium periodate, hydrogen peroxide, urea peroxide, alkali metal bromates, cumene hydroperoxide, tert-butyl peroxide, peracids such as performic acid, peracetic acid, pertrifluoroacetic acid, perbenzoic acid, m-chloroperbenzoic acid, o-carboxyperbenzoic acid and the like; sodium metaperiodate, bichromic acid; bichromates such as sodium bichromate, potassium bichromate; permanganic acid; permanganates such as potassium permanganate, sodium permanganate; and lead salts such as lead tetraacetate.

VI. Alternative Compound Forms or Derivatives [0574] Compounds contemplated herein are described with reference to both generic formulae and specific compounds. In addition, invention compounds may exist in a number of different forms or derivatives, all within the scope of the present invention. These include, for example, tautomers, stereoisomers, racemic mixtures, regioisomers, salts, prodrugs (e.g., carboxylic acid esters), solvated forms, different crystal forms or polymorphs, and active metabolites.

(a) Tautomers, Stereoisomers, Regioisomers, and Solvated Forms 105751 It is understood that some compounds may exhibit tautomerism. In such cases, the formulae provided herein expressly depict only one of the possible tautomeric forms. It is therefore to be understood that the formulae provided herein are intended to represent any tautomeric form of the depicted compounds and are not to be limited merely to the specific tautomeric form depicted by the drawings of the formulae.

[0576] Likewise, some of the compounds according to the present invention may exist as stereoisomers, i.e. having the same atomic connectivity of covalently bonded atoms yet differing in the spatial orientation of the atoms. For example, compounds maybe optical stereoisomers, which contain one or more chiral centers, and therefore, may exist in two or more stereoisomeric forms (e.g.
enantiomers or diastereomers). Thus, such compounds may be present as single stereoisomers (i.e., essentially free of other stereoisomers), racemates, and/or mixtures of enantiomers and/or diastereomers. As another example, stereoisomers include geometric isomers, such as cis- or trans-orientation of substituents on adjacent carbons of a double bond. All such single stereoisomers, racemates and mixtures thereof are intended to be within the scope of the present invention. Unless specified to the contrary, all such steroisomeric forms are included within the formulae provided herein.

[0577] In some embodiments, a chiral compound of the present invention is in a form that contains at least 80% of a single isomer (60% enantiomeric excess ("e.e.") or diastereomeric excess ("d.e.")), or at least 85% (70% e.e. or d.e.), 90% (80% e.e. or d.e.), 95% (90% e.e. or d.e.), 97.5% (95% e.e. or d.e.), or 99% (98% e.e. or d.e.). As generally understood by those skilled in the art, an optically pure compound having one chiral center is one that consists essentially of one of the two possible enantiomers (i.e., is enantiomerically pure), and an optically pure compound having more than one chiral center is one that is both diastereomerically pure and enantiomerically pure. In some embodiments, the compound is present in optically pure form.

[0578] For compounds in which synthesis involves addition of a single group at a double bond, particularly a carbon-carbon double bond, the addition may occur at either of the double bond-linked atoms. For such compounds, the present invention includes both such regioisomers.

[0579] Additionally, the formulae are intended to cover solvated as well as unsolvated forms of the identified structures. For example, the indicated structures include both hydrated and non-hydrated forms. Other examples of solvates include the structures in combination with a suitable solvent such as isopropanol, ethanol, methanol, DMSO, ethyl acetate, acetic acid, or ethanolamine.

(b) Prodrugs and Metabolites [0580] In addition to the present formulae and compounds described herein, the invention also includes prodrugs (generally pharmaceutically acceptable prodrugs), active metabolic derivatives (active metabolites), and their pharmaceutically acceptable salts.

[05811 Prodrugs are compounds or pharmaceutically acceptable salts thereof which, when metabolized under physiological conditions or when converted by solvolysis, yield the desired active compound. Prodrugs include, without limitation, esters, amides, carbamates, carbonates, ureides, solvates, or hydrates of the active compound. Typically, the prodrug is inactive, or less active than the active compound, but may provide one or more advantageous handling, administration, and/or metabolic properties. For example, some prodrugs are esters of the active compound; during metabolysis, the ester group is cleaved to yield the active drug. Also, some prodrugs are activated enzymatically to yield the active compound, or a compound which, upon further chemical reaction, yields the active compound.

[0582] In this context, a common example or a prodrug is an alkyl ester of a carboxylic acid.
Relative to compounds of Formula I, further examples include, without limitation, an amide or carbamate derivative at the 1-position nitrogen of the azaindole core.

[0583] As described in The Practice of Medicinal Chemistry, Ch. 31-32 (Ed.
Wermuth, Academic Press, San Diego, CA, 2001), prodrugs can be conceptually divided into two non-exclusive categories, bioprecursor prodrugs and carrier prodrugs. Generally, bioprecursor prodrugs are compounds that are inactive or have low activity compared to the corresponding active drug compound, that contain one or more protective groups and are converted to an active form by metabolism or solvolysis. Both the active drug form and any released metabolic products should have acceptably low toxicity. Typically, the formation of active drug compound involves a metabolic process or reaction that is one of the follow types:

[0584] Oxidative reactions: Oxidative reactions are exemplified without limitation to reactions such as oxidation of alcohol, carbonyl, and acid functionalities, hydroxylation of aliphatic carbons, hydroxylation of alicyclic carbon atoms, oxidation of aromatic carbon atoms, oxidation of carbon-carbon double bonds, oxidation of nitrogen-containing functional groups, oxidation of silicon, phosphorus, arsenic, and sulfur, oxidative N-dealkylation, oxidative 0- and S-dealkylation, oxidative deamination, as well as other oxidative reactions.

[0585] Reductive reactions: Reductive reactions are exemplified without limitation to reactions such as reduction of carbonyl functionalities, reduction of alcholo functionalities and carbon-carbon double bonds, reduction of nitrogen-containing function groups, and other reduction reactions.
[0586] Reactions without change in the oxidation state: Reactions without change in the state of oxidation are exemplified without limitation to reactions such as hydrolysis of esters and ethers, hydrolytic cleavage of carbon-nitrogen single bonds, hydrolytic cleavage of non-aromatic heterocycles, hydration and dehydration at multiple bonds, new atomic linkages resulting from dehydration reactions, hydrolytic dehalogenation, removal of hydrogen halide molecule, and other such reactions.

[0587] Carrier prodrugs are drug compounds that contain a transport moiety, e.g., that improves uptake and/or localized delivery to a site(s) of action. Desirably for such a carrier prodrug, the linkage between the drug moiety and the transport moiety is a covalent bond, the prodrug is inactive or less active than the drug compound, the prodrug and any release transport moiety are acceptably non-toxic. For prodrugs where the transport moiety is intended to enhance uptake, typically the release of the transport moiety should be rapid. In other cases, it is desirable to utilize a moiety that provides slow release, e.g., certain polymers or other moieties, such as cyclodextrins. (See, e.g., Cheng et al., U.S. Patent Publ. No. 20040077595, App. No. 10/656,838. Such carrier prodrugs are often advantageous for orally administered drugs. Carrier prodrugs can, for example, be used to improve one or more of the following properties: increased lipophilicity, increased duration of pharmacological effects, increased site-specificity, decreased toxicity and adverse reactions, and/or improvement in drug formulation (e.g., stability, water solubility, suppression of an undesirable organoleptic or physiochemical property). For example, lipophilicity can be increased by esterification of hydroxyl groups with lipophilic carboxylic acids, or of carboxylic acid groups with alcohols, e.g., aliphatic alcohols. Wermuth, supra.
[0588] Prodrugs may proceed from prodrug form to active form in a single step or may have one or more intermediate forms which may themselves have activity or may be inactive.
[0589] Metabolites, e.g., active metabolites overlap with prodrugs as described above, e.g., bioprecursor prodrugs. Thus, such metabolites are pharmacologically active compounds or compounds that further metabolize to pharmacologically active compounds that are derivatives resulting from metabolic process in the body of a subject. Of these, active metabolites are such pharmacologically active derivative compounds. For prodrugs, the prodrug compound is generally inactive or of lower activity than the metabolic product. For active metabolites, the parent compound may be either an active compound or may be an inactive prodrug.

[0590] Prodrugs and active metabolites may be identified using routine techniques know in the art.
See, e.g., Bertolini et al, 1997, JMed Chen 40:2011-2016; Shan et al., JPharm Sci 86:756-757;
Bagshawe, 1995, Drug Dev Res 34:220-230; Wermuth, supra.

(c) Pharmaceutically acceptable salts [0591] Compounds can be formulated as or be in the form of pharmaceutically acceptable salts.
Contemplated pharmaceutically acceptable salt forms include, without limitation, mono, bis, tris, tetrakis, and so on, Pharmaceutically acceptable salts are non-toxic in the amounts and concentrations at which they are administered. The preparation of such salts can facilitate the pharmacological use by altering the physical characteristics of a compound without preventing it from exerting its physiological effect. Useful alterations in physical properties include lowering the melting point to facilitate transmucosal administration and increasing the solubility to facilitate administering higher concentrations of the drug.

[0592] Pharmaceutically acceptable salts include acid addition salts such as those containing sulfate, chloride, hydrochloride, fumarate, maleate, phosphate, sulfamate, acetate, citrate, lactate, tartrate, methanesulfonate, ethanesulfonate, benzenesulfonate,p-toluenesulfonate, cyclohexylsulfamate and quinate. Pharmaceutically acceptable salts can be obtained from acids such as hydrochloric acid, maleic acid, sulfuric acid, phosphoric acid, sulfamic acid, acetic acid, citric acid, lactic acid, tartaric acid, malonic acid, methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, cyclohexylsulfamic acid, fumaric acid, and quinic acid.

[0593] Pharmaceutically acceptable salts also include basic addition salts such as those containing benzathine, chloroprocaine, choline, diethanolamine, ethanolamine, t-butylamine, ethylenediamine, meglumine, procaine, aluminum, calcium, lithium, magnesium, potassium, sodium, ammonium, alkylamine, and zinc, when acidic functional groups, such as carboxylic acid or phenol are present.
For example, see Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Co., Easton, PA, Vol. 2, p. 1457, 1995. Such salts can be prepared using the appropriate corresponding bases.

[0594] Pharmaceutically acceptable salts can be prepared by standard techniques. For example, the free-base form of a compound can be dissolved in a suitable solvent, such as an aqueous or aqueous-alcohol solution containing the appropriate acid and then isolated by evaporating the solution. In another example, a salt can be prepared by reacting the free base and acid in an organic solvent.
[0595] Thus, for example, if the particular compound is a base, the desired pharmaceutically acceptable salt may be prepared by any suitable method available in the art, for example, treatment of the free base with an inorganic acid, such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like, or with an organic acid, such as acetic acid, maleic acid, succinic acid, mandelic acid, fumaric acid, malonic acid, pyruvic acid, oxalic acid, glycolic acid, salicylic acid, a pyranosidyl acid, such as glucuronic acid or galacturonic acid, an alpha-hydroxy acid, such as citric acid or tartaric acid, an amino acid, such as aspartic acid or glutamic acid, an aromatic acid, such as benzoic acid or cinnamic acid, a sulfonic acid, such as p-toluenesulfonic acid or ethanesulfonic acid, or the like.

[0596] Similarly, if the particular compound is an acid, the desired pharmaceutically acceptable salt may be prepared by any suitable method, for example, treatment of the free acid with an inorganic or organic base, such as an amine (primary, secondary or tertiary), an alkali metal hydroxide or alkaline earth metal hydroxide, or the like. Illustrative examples of suitable salts include organic salts derived from amino acids, such as L-glycine, L-Iysine, and L-arginine, ammonia, primary, secondary, and tertiary amines, and cyclic amines, such as hydroxyethylpyrrolidine, piperidine, morpholine or piperazine, and inorganic salts derived from sodium, calcium, potassium, magnesium, manganese, iron, copper, zinc, aluminum and lithium. Further examples of pharmaceutically acceptable salts of compounds of Formulae I-III include, without limitation, the mono-sodium and bis-potassium salts thereof.

[0597] The pharmaceutically acceptable salt of the different compounds may be present as a complex. Examples of complexes include 8-chlorotheophylline complex (analogous to, e.g., dimenhydrinate: diphenhydramine 8-chlorotheophylline (1:1) complex; Dramamine) and various cyclodextrin inclusion complexes.

[0598] Unless specified to the contrary, specification of a compound herein includes pharmaceutically acceptable salts of such compound.

(d) Polymorphic forms [0599] In the case of agents that are solids, it is understood by those skilled in the art that the compounds and salts may exist in different crystal or polymorphic forms, all of which are intended to be within the scope of the present invention and specified formulae.

VII. Administration [0600] The methods and compounds will typically be used in therapy for human subjects.
However, they may also be used to treat similar or identical indications in other animal subjects. In this context, the terms "subject," "animal subject," and the like refer to human and non-human vertebrates, e.g. mammals, such as non-human primates, sports and commercial animals, e.g., equines, bovines, porcines, ovines, rodents, and pets, e.g., canines and felines.

[0601] Suitable dosage forms, in part, depend upon the use or the route of administration, for example, oral, transdermal, transmucosal, inhalant or by injection (parenteral). Such dosage forms should allow the compound to reach target cells. Other factors are well known in the art, and include considerations such as toxicity and dosage forms that retard the compound or composition from exerting its effects. Techniques and formulations generally may be found in The Science and Practice of Pharmacy, 215` edition, Lippincott, Williams and Wilkins, Philadelphia, PA, 2005.

[0602] Compounds of the present invention, i.e. Formula I, can be formulated as pharmaceutically acceptable salts.

[0603] Carriers or excipients can be used to produce compositions. The carriers or excipients can be chosen to facilitate administration of the compound. Examples of carriers include calcium carbonate, calcium phosphate, various sugars such as lactose, glucose, or sucrose, or types of starch, cellulose derivatives, gelatin, vegetable oils, polyethylene glycols and physiologically compatible solvents. Examples of physiologically compatible solvents include sterile solutions of water for injection (WFI), saline solution, and dextrose.

[0604] The compounds can be administered by different routes including intravenous, intraperitoneal, subcutaneous, intramuscular, oral, transmucosal, rectal, transdermal, or inhalant. Oral administration is preferred. For oral administration, for example, the compounds can be formulated into conventional oral dosage forms such as capsules, tablets, and liquid preparations such as syrups, elixirs, and concentrated drops.

[0605) For inhalants, compounds of the invention may be formulated as dry powder or a suitable solution, suspension, or aerosol. Powders and solutions may be formulated with suitable additives known in the art. For example, powders may include a suitable powder base such as lactose or starch, and solutions may comprise propylene glycol, sterile water, ethanol, sodium chloride and other additives, such as acid, alkali and buffer salts. Such solutions or suspensions may be administered by inhaling via spray, pump, atomizer, or nebulizer, and the like. The compounds of the invention may also be used in combination with other inhaled therapies, for example corticosteroids such as fluticasone proprionate, beclomethasone dipropionate, triamcinolone acetonide, budesonide, and mometasone furoate; beta agonists such as albuterol, salmeterol, and formoterol; anticholinergic agents such as ipratroprium bromide or tiotropium; vasodilators such as treprostinal and iloprost;
enzymes such as DNAase; therapeutic proteins; immunoglobulin antibodies; an oligonucleotide, such as single or double stranded DNA or RNA, siRNA; antibiotics such as tobramycin; muscarinic receptor antagonists; leukotriene antagonists; cytokine antagonists; protease inhibitors; cromolyn sodium; nedocril sodium; and sodium cromoglycate.

[0606] Pharmaceutical preparations for oral use can be obtained, for example, by combining the active compounds with solid excipients, optionally grinding a resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries, if desired, to obtain tablets or dragee cores.
Suitable excipients are, in particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethyl-cellulose, sodium carboxymethylcellulose (CMC), and/or polyvinylpyrrolidone (PVP: povidone). If desired, disintegrating agents may be added, such as the cross-linked polyvinylpyrrolidone, agar, or alginic acid, or a salt thereof such as sodium alginate.

[0607J Dragee cores are provided with suitable coatings. For this purpose, concentrated sugar solutions may be used, which may optionally contain, for example, gum arabic, talc, poly-vinylpyrrolidone, carbopol gel, polyethylene glycol (PEG), and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dye-stuffs or pigments may be added to the tablets or dagee coatings for identification or to characterize different combinations of active compound doses.

[06081 Pharmaceutical preparations that can be used orally include push-fit capsules made of gelatin ("gelcaps"), as well as soft, sealed capsules made of gelatin, and a plasticizer, such as glycerol or sorbitol. The push-fit capsules can contain the active ingredients in admixture with filler such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active; compounds may be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycols (PEGs). In addition, stabilizers may be added.

[06091 Alternatively, injection (parenteral administration) may be used, e.g., intramuscular, intravenous, intraperitoneal, and/or subcutaneous. For injection, the compounds of the invention are formulated in sterile liquid solutions, preferably in physiologically compatible buffers or solutions, such as saline solution, Hank's solution, or Ringer's solution. In addition, the compounds may be formulated in solid form and redissolved or suspended immediately prior to use. Lyophilized forms can also be produced.

[06101 Administration can also be by transmucosal, topical, or transdermal means. For transmucosal, topical or transdermal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art, and include, for example, for transmucosal administration, bile salts and fusidic acid derivatives. In addition, detergents may be used to facilitate permeation. Transmucosal administration, for example, may be through nasal sprays or suppositories (rectal or vaginal). The topical compositions of this invention are formulated preferably as oils, creams, lotions, ointments and the like by choice of appropriate carriers known in the art. Suitable carriers include vegetable or mineral oils, white petrolatum (white soft paraffin), branched chain fats or oils, animal fats and high molecular weight alcohol (greater than C12). The preferred carriers are those in which the active ingredient is soluble. Emulsifiers, stabilizers, humectants and antioxidants may also be included as well as agents imparting color or fragrance, if desired. Creams for topical application are preferably formulated from a mixture of mineral oil, self-emulsifying beeswax and water in which mixture the active ingredient, dissolved in a small amount solvent (e.g., an oil), is admixed. Additionally, administration by transdermal means may comprise a transdermal patch or dressing such as a bandage impregnated with an active ingredient and optionally one or more carriers or diluents known in the art.
To be administered in the form of a transdermal delivery system, the dosage administration will, of course, be continuous rather than intermittent throughout the dosage regimen.

[0611] The amounts of various compounds to be administered can be determined by standard procedures taking into account factors such as the compound IC50, the biological half-life of the compound, the age, size, and weight of the subject, and the disorder associated with the subject. The importance of these and other factors are well known to those of ordinary skill in the art. Generally, a dose will be between about 0.01 and 50 mg/kg, preferably 0.1 and 20 mg/kg of the subject being treated. Multiple doses may be used.

[0612] The compounds of the invention may also be used in combination with other therapies for treating the same disease. Such combination use includes administration of the compounds and one or more other therapeutics at different times, or co-administration of the compound and one or more other therapies. In some embodiments, dosage may be modified for one or more of the compounds of the invention or other therapeutics used in combination, e.g., reduction in the amount dosed relative to a compound or therapy used alone, by methods well known to those of ordinary skill in the art.
[06131 It is understood that use in combination includes use with other therapies, drugs, medical procedures etc., where the other therapy or procedure may be administered at different times (e.g.
within a short time, such as within hours (e.g. 1, 2, 3, 4-24 hours), or within a longer time (e.g. 1-2 days, 2-4 days, 4-7 days, 1-4 weeks)) than a compound of the present invention, or at the same time as a compound of the invention. Use in combination also includes use with a therapy or medical procedure that is administered once or infrequently, such as surgery, along with a compound of the invention administered within a short time or longer time before or after the other therapy or procedure. In some embodiments, the present invention provides for delivery of compounds of the invention and one or more other drug therapeutics delivered by a different route of administration or by the same route of administration. The use in combination for any route of administration includes delivery of compounds of the invention and one or more other drug therapeutics delivered by the same route of administration together in any formulation, including formulations where the two compounds are chemically linked in such a way that they maintain their therapeutic activity when administered. In one aspect, the other drug therapy may be co-administered with one or more compounds of the invention. Use in combination by co-administration includes administration of co-formulations or formulations of chemically joined compounds, or administration of two or more compounds in separate formulations within a short time of each other (e.g.
within an hour, 2 hours, 3 hours, up to 24 hours), administered by the same or different routes. Co-administration of separate formulations includes co-administration by delivery via one device, for example the same inhalant device, the same syringe, etc., or administration from separate devices within a short time of each other. Co-formulations of compounds of the invention and one or more additional drug therapies delivered by the same route includes preparation of the materials together such that they can be administered by one device, including the separate compounds combined in one formulation, or compounds that are modified such that they are chemically joined, yet still maintain their biological activity. Such chemically joined compounds may have a linkage that is substantially maintained in vivo, or the linkage may break down in vivo, separating the two active components.

EXAMPLES
[0614] Examples related to the present invention are described below. In most cases, alternative techniques can be used. The examples are intended to be illustrative and are not limiting or restrictive to the scope of the invention. In some examples, the mass spectrometry result indicated for a compound may have more than one value due to the isotope distribution of an atom in the molecule, such as a compound having a bromo or chloro substituent.

Example 1: Synthesis of propane-l-sulfonic acid [3-(5-bromo-lII-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-amide P-0773 and related compounds.

[0615] Compound P-0773 was synthesized in five steps from 2,4-difluoro-phenylamine 42 as shown in Scheme 13.

Scheme 13 F O F O F O F
Step 1 BnO Step 2 BnO r Step 3 HO *
FJ F'~)I - F F

O O
O F F
CI Br \ Step 5 Step 4 + O O
F O N H Br i F HN J-HNp~\ 67 \N I N O

Step I - Preparation of 3-amino-2, 6-difluoro-benzoic acid benzyl ester (43):
[0616] To 2,4-difluoro-phenylamine (42, 5.11 mL, 50.7 mmol) in tetrahydrofuran (250 mL), cooled with dry ice/acetone bath under an atmosphere of nitrogen, was added n-butyllithium (1.60 M in hexane, 34.0 mL, 54.4 mmol) slowly. After 30 minutes, 1,2-Bis-(chloro-dimethyl-silanyl)-ethane (11.5 g, 53.4 mmol) dissolved in tetrahydrofuran (40.0 mL) was added to the reaction slowly. After 1 hour, n-butyllithium (1.60 M in hexane, 31.9 mL, 51.0 mmol) was added slowly to the reaction. The reaction was stirred at -78 C for 30 minutes and then allowed to warm to room temperature over 40 minutes. The reaction was cooled to -78 C, followed by addition of n-butyllithium (1.60 M in hexane, 35.1 mL, 56.2 mmol) slowly. After 70 minutes, benzyl chloroformate (7.97 mL, 55.8 mmol) was added to the reaction. The reaction mixture was stirred at -78 C
overnight followed by addition of 2 N HCl (120 mL). The reaction was allowed to warm to room temperature for 2 hours. The organic layer was separated. The aqueous layer was basified with potassium carbonate and extracted with ethyl acetate. The organic layers were combined and washed with brine, dried over anhydrous sodium sulfate, filtrated and concentrated. The desired compound was isolated by silica gel column chromatography (ethyl acetate/hexane 20%) to give a colorless oil (43, 10.6 g, 79.7%). MS(ESI) [M+I-I}]+ = 264.1.

Step 2 - Preparation of 2, 6-dfluoro-3-(propane-l -sulfonylamino)-benzoic acid benzyl ester (44):
[0617] To 3-amino-2,6-difluoro-benzoic acid benzyl ester (43, 6.00 g, 22.8 mmol) in methylene chloride (150 mL) was added pyridine (2.76 mL, 34.2 mmol) and propane-l-sulfonyl chloride (3.80 mL, 33.8 mmol). The reaction was stirred at room temperature overnight. Then the reaction was poured into water, and extracted with ethyl acetate. The organic layer was washed with brine, dried over anhydrous sodium sulfate, filtrated and concentrated. The desired compound was isolated with silica gel column chromatography to give a colorless oil (44, 7.0 g, 83.1 %).
MS(ESI) [M+H+]+ _ 370.1.

Step 3 - Preparation of2, 6-difluoro-3-(propane-l-sulfonylamino)-benzoic acid (45):
[0618] To 2,6-difluoro-3-(propane-1 -sulfonylamino)-benzoic acid benzyl ester (44, 2.0 g, mmol) in methanol (30 mL) was added 20% palladium hydroxide on carbon (100 mg). The reaction was stirred under hydrogen at 1 atm for 15 minutes. The reaction was filtrated and concentrated to give white solid 45 that was used in the next step.

Step 4 - Preparation of 2, 6-dfuoro-3-(propane-l -sulfonylamino)-benzoyl chloride (46):
[0619] To 2,6-difluoro-3-(propane-l-sulfonylamino)-benzoic acid (45, 1.50 g, 5.4 mmol) was added toluene (7.0 mL) and thionyl chloride (15.0 mL, 0.21 mmol). The reaction was heated to reflux for 3 hours. The reaction was concentrated to give crude compound that was used in the next step.

Step 5- Preparation ofpropane-l-sulfonic acid [3-(5-bromo-IH-pyrrolo[2,3-bJpyridine-3-carbonyl)-2,4-difluoro phenylJ-amide (P-0773):
[0620] To aluminum trichloride (8.89 g, 66.7 mmol) was added methylene chloride (150 mL) under an atmosphere of nitrogen below 5 C. Into this, 5-bromo-7-azaindole (67, 1.64 g, 8.34 mmol) in methylene chloride (20 mL) was added. The reaction was stirred for 60.0 minutes and 2,6-difluoro-3-(propane-1 -sulfonylamino)-benzoyl chloride (46, 3.50 g, 11.8 mmol) in methylene chloride (20 mL) was added. The reaction was stirred for 6 hours and warmed to room temperature overnight. The reaction mixture was poured into water and extracted with ethyl acetate. The organic layer was dried over anhydrous sodium sulfate, filtrated and concentrated. The desired compound was isolated by silica gel column chromatography (methylene chloride/methanol 5%) to give a white solid (P-0773, 1.2 g, 31.4%). MS(ESI) [M+H+]+ = 460.0, 462Ø

[0621] N-[3-(5-Bromo-lH-pyrrolo[2,3-b]pyridine-3-carbonyl)-4-chloro-2-fluoro-phenyl]-methanesulfonamide P-0868 CI

O
Br F HN-S-N H O

was prepared following the protocol of Scheme 13, substituting propane-l-sulfonyl chloride with methanesulfonyl chloride in Step 2 and 2,4-difluoro-phenylamine with 4-chloro-2-fluoro-phenylamine in Step 1. MS (ESI) [M - H+]" = 443.9, 445.9.

[0622] N-[3-(5-Bromo-lH-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-methanesulfonamide P-0162 F
O
Br O
F HN-S-N N
H
was prepared following the protocol of Scheme 13, substituting propane-l-sulfonyl chloride with methanesulfonyl chloride in Step 2. This was isolated and reacted further (50 mg, 0.10 mmol) in methanol (20 mL) by adding 20% palladium hydroxide on carbon (53 mg). The reaction was stirred under hydrogen at 40 psi for 12 hours. The reaction mixture was filtered and concentrated to give product methanesulfonic acid (2,4-difluoro-3-lH-pyrrolo-[2,3-b]pyridine-3-carbonyl)-phenyl]-amide P-0811 (40 mg, 95%).
F
O
O
C-11 \ F HN-S-N H O
MS(ESI)[M+H+]+ = 352.3.

[0623] N-[3-(5-Bromo-lH-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-benzenesulfonamide P-0798 and N-[3-(5-bromo-lH-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl] -3 -fluoro-benzenesulfonamide F F
O F
p O O
Br F HN-S Br \ F HN'S \ /

N H P-0798 O and \N H O

were prepared following the protocol of Scheme 13, substituting propane-l-sulfonyl chloride with benzenesulfonyl chloride and 3-fluoro-benzenesulfonyl chloride, respectively, in Step 2. P-0798 MS(ESI) [M - H{]- = 489.9, 491.9.

[06241 Propane- I -sulfonic acid [3-(5-bromo-lH-pyrrolo[2,3-b]pyridine-3-carbonyl)-4-chloro-2-fluoro-phenyl]-amide P-0805 CI
O
Br O
1 F HN-S--\_ N N O
H
was prepared following the protocol of Scheme 13, substituting 2,4-difluoro-phenylamine with 4-chlQro-2-fluoro-phenylamine in Step 1. MS(ESI) [M - H+]" = 471.9, 473.9.

[06251 Propane- l-sulfonic acid [2,4-difluoro-3-(1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-phenyl]-amide P-0007 F
O
O
F HN-S-\
`N N 0 was prepared following the protocol of Scheme 13, substituting 5-bromo-7-azaindole with 7-azaindole in Step 5. MS(ESI) [M + W]+ = 380.1.

106261 Propane- l-sulfonic acid [2,4-difluoro-3-(5-methoxy-lH-pyrrolo[2,3-b]pyridine-3-carbonyl)-phenyl]-amide P-0806 F
O //
'O n\ F HN-S--\\.
O
N H O

was prepared following the protocol of Scheme 13, substituting 5-bromo-7-azaindole with 5-methoxy-7-azaindole 104 (prepared as described in Example 16) in Step 5.
MS(ESI) [M -410.1.

[06271 (5-Bromo-IH-pyrrolo[2,3-b]pyridin-3-yl)-(2-fluoro-3-methoxy-phenyl)-methanone P-0265 O
Br O\ F
N N
H
was prepared using the protocol of steps 4 and 5 of Scheme 13, substituting 2,6-difluoro-3-(propane-1-sulfonylamino)-benzoic acid with 2-fluoro-3-methoxy-benzoic acid in Step 4.
MS (ESI) [M - H+]"
347, 349.

[06281 N-[3-(5-Bromo-lH-pyrrolo[2,3-b]pyridine-3-carbonyl)-4-fluoro phenyl]-methanesulfonamide P-0170 F
O
O
Br r \ HN-S-N H

was prepared using the protocol of steps 2, 4, and 5 of Scheme 13, substituting 3-amino-2,6-difluoro-benzoic acid benzyl ester with 5-amino-2-fluoro-benzoic acid and propane-l-sulfonyl chloride with methanesulfonyl chloride in Step 2, to provide the acid that is carried through in Step 4. MS (ESI) [M
-W]`=410.0,412Ø

[0629] N-[3-(5-Bromo-lH-pyrrolo[2,3-b]pyridine-3-carbonyl)-2-methyl-phenyl]-methanesulfonamide P-0180 O Il Br O
HN-S-N H

was prepared using the protocol of steps 2, 4, and 5 of Scheme 13, substituting 3-amino 2,6-difluoro-benzoic acid benzyl ester with 3-amino-2-methyl-benzoic acid and propane- l-sulfonyl chloride with methanesulfonyl chloride in Step 2, to provide the acid that is carried through in Step 4. MS (ESI) [M
- H*)- = 405.9, 407.9.

[0630] (5-Bromo-lH-pyrrolo[2,3-b]pyridin-3-yl)-(3-chloro-2-fluoro-phenyl)-methanone P-0299 O

Br \ F CI
N N
H
was prepared using the protocol of Step 5 of Scheme 13, substituting 2,6-difluoro-3-(propane-l-sulfonylamino)-benzoyl chloride with 3-chloro-2-fluoro-benzoyl chloride. MS
(ESI) [M - H*]' 350.9, 352.9.

Example 2: Synthesis of Propane-l-sulfonic acid [3-(5-bromo-lH-pyrrolo[2,3-b]pyridine-3-carbonyl)-2-fluoro-phenyl]-amide P-0955 and related compounds.

[0631] Compound P-0955 was synthesized in six steps from 4-chloro-2-fluoro-phenylamine 47 as shown in Scheme 14.

Scheme 14 Step 1 Bn0 r I Step 2 BnO Step 3 HO I Step 4 F 'O F' ~ F F ~
HNC N. 0 47 48 49 :ep HO Step 5 1 + Br \ 6 Br HN-O
Step 1- Preparation of 3 Anzino-6-chloro-2 fluoro-benzoic acid benzyl ester (48):
[0632] To 4-chloro-2-fluoro-phenylamine (47, 6.30 mL, 57.0 mmol) in tetrahydrofuran (300 mL), cooled with dry ice/acetone bath under an atmosphere of nitrogen, was added n-butyllithium (2.500 M
in hexane, 24.4 niL) slowly. After 20 minutes, 1,2 Bis-(chloro-dimethyl-silanyl)-ethane (12.9 g, 60.0 mmol) dissolved in tetrahydrofuran (40.0 mL) was added to the reaction slowly.
After 1 hour, n-butyllithium (2.50 M in hexane, 25.0 mL) was added slowly to the reaction. The reaction was stirred at -78 C for 20 minutes and allowed to warm to room temperature over 60 minutes. The reaction was cooled to -78 C, followed by addition of n-butyllithium (2.50 M in hexane, 26.0 mL) slowly.
After 80 minutes, benzyl chloroformate (10.0 mL, 70.0 mmol) was added to the reaction. The reaction mixture was stirred at -78 C overnight followed by addition of water (80 mL) and concentrated hydrochloric acid (25 mL). The reaction was allowed to warm to room temperature for 2 hours. The organic layer was separated and the aqueous layer was basified with potassium carbonate and extracted with ethyl acetate. The organic layers were combined and washed with brine, dried over anhydrous sodium sulfate, filtrated and concentrated. The desired compound was isolated by silica gel column chromatography (ethyl acetate/hexane 20%) to give a colorless oil (48, 12.5 g, 78.3%). MS(ESI) [M+H+]+ = 280Ø

Step 2 - Preparation of 6-chloro-2 fluoro-3-(propane-l -sulfonylanzino)-benzoic acid benzyl ester (49):
[0633] To 3-amino-6-chloro-2-fluoro-benzoic acid benzyl ester (48, 1.20 g, 4.3 mmol) in methylene chloride (28 mL) was added pyridine (0.52 mL, 6.4 mmol) and propane-l-sulfonyl chloride (0.685 g, DEMANDES OU BREVETS VOLUMINEUX
LA PRESENTE PARTIE DE CETTE DEMANDE OU CE BREVETS
COMPREND PLUS D'UN TOME.

NOTE: Pour les tomes additionels, veillez contacter le Bureau Canadien des Brevets.

JUMBO APPLICATIONS / PATENTS

THIS SECTION OF THE APPLICATION / PATENT CONTAINS MORE
THAN ONE VOLUME.

THIS IS VOLUME OF

NOTE: For additional volumes please contact the Canadian Patent Office.

Claims (70)

1. A compound having the structure of Formula IIIm:
or a pharmaceutically acceptable salt thereof, wherein:
R81 is selected from the group consisting of hydrogen, halogen, optionally substituted C1-C6 alkyl, optionally substituted C2-C6 alkenyl, optionally substituted C2-C6 alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -OH, -NH2, -CN, -NO2, -C(O)OH, -S(O)2NH2, -C(O)NH2, -C(S)NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)OR68, -C(O)NR69R68, -C(S)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, -NR69C(S)R68, -NR69S(O)2R68, -NR 69C(O)NH2, -NR69C(O)NR69R68, -NR69C(S)NH2, -NR69C(S)NR69R68, -NR69S(O)2NH2, -NR69S(O)2NR69R68, -S(O)R68, and -S(O)2R68;

R83 is selected from the group consisting of hydrogen, fluoro and chloro;
R112 is selected from the group consisting of optionally substituted C2-6 alkyl, optionally substituted aryl, optionally substituted heteroaryl, and -NR79R80;
R68 is selected from the group consisting of optionally substituted C1-C6 alkyl, optionally substituted C2-C6 alkenyl, provided, however, that when R68 is optionally substituted C2-C6 alkenyl, no alkene carbon thereof is bound to N, S, O, S(O), S(O)2, C(O) or C(S) of -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)OR68, -C(O)NR69R68, -C(S)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, -NR69C(S)R68, -NR69S(O)2R68, -NR69C(O)NH2, -NR69C(O)NR69R68, -NR69C(S)NH2, -NR69C(S)NR69R68, -NR69S(O)2NH2, -NR69S(O)2NR69R68, -S(O)R68, or -S(O)2R68, optionally substituted C2-C6 alkynyl, provided, however, that when R68 is optionally substituted C2-C6 alkynyl, no alkyne carbon thereof is bound to N, S, O, S(O), S(O)2, C(O) or C(S) of -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)OR68, -C(O)NR 69R68, -C(S)NR69R68, -S(O)2NR69R68, -NR69C(O)R68, -NR69C(S)R68, -NR69S(O)2R68, -NR61C(O)NH2, -NR69C(O)NR69R68, -NR69C(S)NH2, -NR69C(S)NR69R68, -NR69S(O)2NH2, -NR69S(O)2NR69R68, -S(O)R68, or -S(O)2R68, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl;
R69 is selected from the group consisting of hydrogen and optionally substituted C1-C6 alkyl; and R79 and R80 are independently hydrogen or optionally substituted C1-C6 alkyl, or R79 and R80 combine with the nitrogen to which they are attached to form optionally substituted 5-7 membered heterocycloalkyl;
wherein:
optionally substituted C1-C6 alkyl as R68, R69, R79, R80, or R81, or C2-6 alkyl as R112, are C1-C6 alkyl or C2-6 alkyl, respectively, optionally substituted with one or more substituents selected from the group consisting of -F, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR o, -SR o, -OC(O)R o, -OC(S)R o, -C(O)R o, -C(S)R
o, -C(O)OR o, -C(S)OR o, -S(O)R o, -S(O)2R o, -C(O)NHR o, -C(S)NHR o, -C(O)NR o R
o, -C(S)NR o R o, -S(O)2NHR o, -S(O)2NR o R o, -C(NH)NHR o, -C(NH)NR p R o, -NHC(O)R o, -NHC(S)R o, -NR o C(O)R o, -NR o C(S)R o, -NHS(O)2R o, -NR o S(O)2R o, -NHC(O)NHR o, -NHC(S)NHR o, -NR o C(O)NH2, -NR o C(S)NH2, -NR o C(O)NHR o, -NR o C(S)NHR o, -NHC(O)NR o R o, -NHC(S)NR o R o, -NR o C(O)NR o R o, -NR o C(S)NR o R o, -NHS(O)2NHR o, -NR o S(O)2NH2, -NR o S(O)2NHR o, -NHS(O)2NR o R o, -NR o S(O)2NR o R o, -NHR
o, -NR o R o, -R e, -R f, and -R g;

optionally substituted C2-C6 alkenyl as R68 or R81 is C2-C6 alkenyl optionally substituted with one or more substituents selected from the group consisting of -F, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR o, -SR o, -OC(O)R o, -OC(S)R o, -C(O)R o, -C(S)R o, -C(O)OR o, -C(S)OR o, -S(O)R o, -S(O)2R o, -C(O)NHR o, -C(S)NHR o, -C(O)NR o R o, -C(S)NR o R o, -S(O)2NHR o, -S(O)2NR o R o, -C(NH)NHR o, -C(NH)NR p R o, -NHC(O)R o, -NHC(S)R o, -NR o C(O)R o, -NR o C(S)R o, -NHS(O)2R o, -NR o S(O)2R o, -NHC(O)NHR o, -NHC(S)NHR o, -NR o C(O)NH2, -NR o C(S)NH2, -NR o C(O)NHR o, -NR o C(S)NHR o, -NHC(O)NR o R o, -NHC(S)NR o R o, -NR o C(O)NR o R o, -NR o C(S)NR o R o, -NHS(O)2NHR o, -NR o S(O)2NH2, -NR o S(O)2NHR o, -NHS(O)2NR o R o, -NR o S(O)2NR o R o, -NHR o, -NR o R o, -R d, -R f, and -R g;

optionally substituted C2-C6 alkynyl as R68 or R81 is C2-C6 alkynyl optionally substituted with one or more substituents selected from the group consisting of -F, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR o, -SR o, -OC(O)R o, -OC(S)R o, -C(O)R o, -C(S)R o, -C(O)OR o, -C(S)OR o, -S(O)R o, -S(O)2R o, -C(O)NHR o, -C(S)NHR o, -C(O)NR o R o, -C(S)NR o R o, -S(O)2NHR o, -S(O)2NR o R o, -C(NH)NHR o, -C(NH)NR p R o, -NHC(O)R o, -NHC(S)R o, -NR o C(O)R o, -NR o C(S)R o, -NHS(O)2R o, -NR o S(O)2R o, -NHC(O)NHR o, -NHC(S)NHR o, -NR o C(O)NH2, -NR o C(S)NH2, -NR o C(O)NHR o, -NR o C(S)NHR o, -NHC(O)NR o R o, -NHC(S)NR o R o, -NR o C(O)NR o R o, -NR o C(S)NR o R o, -NHS(O)2NHR o, -NR o S(O)2NH2, -NR o S(O)2NHR o, -NHS(O)2NR o R o, -NR o S(O)2NR o R o, -NHR o, -NR o R o, -R d, -R e, and -R g;
optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl or optionally substituted heteroaryl, as R68, R81, R112, or optionally substituted 5-7 membered heterocycloalkyl as R79 and R80 combined with the nitrogen to which they are attached are cycloalkyl, heterocycloalkyl, aryl, heteroaryl or 5-7 membered heterocycloalkyl, respectively, each of which is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR o, -SR o, -OC(O)R o, -OC(S)R o, -C(O)R o, -C(S)R o, -C(O)OR o, -C(S)OR o, -S(O)R o, -S(O)2R o, -C(O)NHR o, -C(S)NHR o, -C(O)NR o R o, -C(S)NR o R o, -S(O)2NHR o, -S(O)2NR o R o, -C(NH)NHR o, -C(NH)NR p R o, -NHC(O)R o, -NHC(S)R o, -NR o C(O)R o, -NR o C(S)R o, -NHS(O)2R o, -NR o S(O)2R o, -NHC(O)NHR o, -NHC(S)NHR o, -NR o C(O)NH2, -NR o C(S)NH2, -NR o C(O)NHR o, -NR o C(S)NHR o, -NHC(O)NR o R o, -NHC(S)NR o R o, -NR o C(O)NR o R o, -NR o C(S)NR o R o, -NHS(O)2NHR o, -NR o S(O)2NH2, -NR o S(O)2NHR o, -NHS(O)2NR o R o, -NR o S(O)2NR o R o, -NHR o, -NR o R o, -R d, -R e, -R f, and -R g;
each R o, R p, and R c are independently selected from the group consisting of R d, R e, R f, and R g, or R p and R o combine with the nitrogen to which they are attached to form a 5-7 membered heterocycloalkyl or a 5 or 7 membered nitrogen containing heteroaryl, wherein the 5-7 membered heterocycloalkyl or 5 or 7 membered nitrogen containing heteroaryl, respectively, are optionally substituted with one or more substituents selected from the group consisting of halogen, -NO2, -CN, -OH, -NH2, -OR u, -SR u, -NHR u, -NR u R u, -R x, and -R y;

each R d is independently C1-C6 alkyl, wherein C1-C6 alkyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR k, -SR k, -OC(O)R k, -OC(S)R k, -C(O)R k, -C(S)R k, -C(O)OR k, -C(S)OR k, -S(O)R k, -S(O)2R k, -C(O)NHR k, -C(S)NHR k, -C(O)NR k R k, -C(S)NR k R k, -S(O)2NHR k, -S(O)2NR k R k, -C(NH)NHR k, -C(NH)NR m R n, -NHC(O)R k, -NHC(S)R k, -NR k C(O)R k, -NR k C(S)R k, -NHS(O)2R k, -NR k S(O)2R k, -NHC(O)NHR k, -NHC(S)NHR k, -NR k C(O)NH2, -NR k C(S)NH2, -NR k C(O)NHR k, -NR k C(S)NHR k, -NHC(O)NR k R k, -NHC(S)NR k R k, -NR k C(O)NR k R k, -NR k C(S)NR k R k, -NHS(O)2NHR k, -NR k S(O)2NH2, -NR k S(O)2NHR k, -NHS(O)2NR k R k, -NR k S(O)2NR k R k, -NHR k, -NR k R k, -R i, and -R j;
each R e is independently C2-C6 alkenyl, wherein C2-C6 alkenyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR k, -SR k, -OC(O)R k, -OC(S)R k, -C(O)R k, -C(S)R k, -C(O)OR k, -C(S)OR k, -S(O)R k, -S(O)2R k, -C(O)NHR k, -C(S)NHR k, -C(O)NR k R k, -C(S)NR k R k, -S(O)2NHR k, -S(O)2NR k R k, -C(NH)NHR k, -C(NH)NR m R n, -NHC(O)R k, -NHC(S)R k, -NR k C(O)R k, -NR k C(S)R k, -NHS(O)2R k, -NR k S(O)2R k, -NHC(O)NHR k, -NHC(S)NHR k, -NR k C(O)NH2, -NR k C(S)NH2, -NR k C(O)NHR k, -NR k C(S)NHR k, -NHC(O)NR k R k, -NHC(S)NR k R k, -NR k C(O)NR k R k, -NR k C(S)NR k R k, -NHS(O)2NHR k, -NR k S(O)2NH2, -NR k S(O)2NHR k, -NHS(O)2NR k R k, -NR k S(O)2NR k R k, -NHR k, -NR k R k, -R h, and -R j;

each R f is independently C2-C6 alkynyl, wherein C2-C6 alkynyl is optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR k, -SR k, -OC(O)R k, -OC(S)R k, -C(O)R k, -C(S)R k, -C(O)OR k, -C(S)OR k, -S(O)R k, -S(O)2R k, -C(O)NHR k, -C(S)NHR k, -C(O)NR k R k, -C(S)NR k R k, -S(O)2NHR k, -S(O)2NR k R k, -C(NH)NHR k, -C(NH)NR m R n, -NHC(O)R k, -NHC(S)R k, -NR k C(O)R k, -NR k C(S)R k, -NHS(O)2R k, -NR k S(O)2R k, -NHC(O)NHR k, -NHC(S)NHR k, -NR k C(O)NH2, -NR k C(S)NH2, -NR k C(O)NHR k, -NR k C(S)NHR k, -NHC(O)NR k R k, -NHC(S)NR k R k, -NR k C(O)NR k R k, -NR k C(S)NR k R k, -NHS(O)2NHR k, -NR k S(O)2NH2, -NR k S(O)2NHR k, -NHS(O)2NR k R k, -NR k S(O)2NR k R k, -NHR k, -NR k R k, -R h, and -R j;

each R g is independently selected from the group consisting of cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, respectively, are optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR k, -SR k, -OC(O)R k, -OC(S)R k, -C(O)R k, -C(S)R
k, -C(O)OR k, -C(S)OR k, -S(O)R k, -S(O)2R k, -C(O)NHR k, -C(S)NHR k, -C(O)NR k R
k, -C(S)NR k R k, -S(O)2NHR k, -S(O)2NR k R k, -C(NH)NHR k, -C(NH)NR m R n, -NHC(O)R k, -NHC(S)R k, -NR k C(O)R k, -NR k C(S)R k, -NHS(O)2R k, -NR k S(O)2R k, -NHC(O)NHR k, -NHC(S)NHR k, -NR k C(O)NH2, -NR k C(S)NH2, -NR k C(O)NHR k, -NR k C(S)NHR k, -NHC(O)NR k R k, -NHC(S)NR k R k, -NR k C(O)NR k R k, -NR k C(S)NR k R k, -NHS(O)2NHR k, -NR k S(O)2NH2, -NR k S(O)2NHR k, -NHS(O)2NR k R k, -NR k S(O)2NR k R k, -NHR
k, -NR k R k, -R h, -R i, and -R j;
R k, R m, and R n at each occurrence are independently selected from the group consisting of R h, R i, and R j or R m and R n combine with the nitrogen to which they are attached form a 5-7 membered heterocycloalkyl or a 5 or 7 membered nitrogen containing heteroaryl, wherein the 5-7 membered heterocycloalkyl or 5 or 7 membered nitrogen containing heteroaryl, respectively, are optionally substituted with one or more substituents selected from the group consisting of halogen, -NO2, -CN, -OH, -NH2, OR u, -SR u, -NHR u, -NR u R u, -R x, and -R y;
each R h is independently C1-C6 alkyl optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR r, -SR r, -OC(O)R r, -OC(S)R r, -C(O)R r, -C(S)R
r, -C(O)OR r, -C(S)OR r, -S(O)R r, -S(O)2R r, -C(O)NHR r, -C(S)NHR r, -C(O)NR r R
r, -C(S)NR r R r, -S(O)2NHR r, -S(O)2NR r R r, -C(NH)NHR r, -C(NH)NR s R t, -NHC(O)R r, -NHC(S)R r, -NR r C(O)R r, -NR r C(S)R r, -NHS(O)2R r, -NR r S(O)2R r, -NHC(O)NHR r, -NHC(S)NHR r, -NR r C(O)NH2, -NR r C(S)NH2, -NR r C(O)NHR r, -NR r C(S)NHR r, -NHC(O)NR r R r, -NHC(S)NR r R r, -NR r C(O)NR r R r, -NR r C(S)NR r R r, -NHS(O)2NHR r, -NR r S(O)2NH2, -NR r S(O)2NHR r, -NHS(O)2NR r R r, -NR r S(O)2NR r R r, -NHR
r, -NR r R r, -R i, and -R j;

each R i is independently selected from the group consisting of C2-C6 alkenyl and C2-C6 alkynyl, wherein C2-C6 alkenyl or C2-C6 alkynyl, respectively, are optionally substituted with one or more substituents selected from the group consisting of fluoro, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR r, -SR r, -OC(O)R r, -OC(S)R r, -C(O)R r, -C(S)R r, -C(O)OR r, -C(S)OR r, -S(O)R r, -S(O)2R r, -C(O)NHR r, -C(S)NHR r, -C(O)NR r R r, -C(S)NR r R r, -S(O)2NHR r, -S(O)2NR r R r, -C(NH)NHR r, -C(NH)NR s R t, -NHC(O)R r, -NHC(S)R r, -NR r C(O)R r, -NR r C(S)R r, -NHS(O)2R r, -NR r S(O)2R r, -NHC(O)NHR r, -NHC(S)NHR r, -NR r C(O)NH2, -NR r C(S)NH2, -NR r C(O)NHR r, -NR r C(S)NHR r, -NHC(O)NR r R r, -NHC(S)NR r R r, -NR r C(O)NR r R r, -NR r C(S)NR r R r, -NHS(O)2NHR r, -NR r S(O)2NH2, -NR r S(O)2NHR r, -NHS(O)2NR r R r, -NR r S(O)2NR r R r, -NHR r, -NR r R r, and -R j;
each R j is independently selected from the group consisting of cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, respectively, are optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, -C(O)OH, -C(S)OH, -C(O)NH2, -C(S)NH2, -S(O)2NH2, -NHC(O)NH2, -NHC(S)NH2, -NHS(O)2NH2, -C(NH)NH2, -OR r, -SR r, -OC(O)R r, -OC(S)R r, -C(O)R r, -C(S)R
r, -C(O)OR r, -C(S)OR r, -S(O)R r, -S(O)2R r, -C(O)NHR r, -C(S)NHR r, -C(O)NR r R
r, -C(S)NR r R r, -S(O)2NHR r, -S(O)2NR r R r, -C(NH)NHR r, -C(NH)NR s R t, -NHC(O)R r, -NHC(S)R r, -NR r C(O)R r, -NR r C(S)R r, -NHS(O)2R r, -NR r S(O)2R r, -NHC(O)NHR r, -NHC(S)NHR r, -NR r C(O)NH2, -NR r C(S)NH2, -NR r C(O)NHR r, -NR r C(S)NHR r, -NHC(O)NR r R r, -NHC(S)NR r R r, -NR r C(O)NR r R r, -NR r C(S)NR r R r, -NHS(O)2NHR r, -NR r S(O)2NH2, -NR r S(O)2NHR r, -NHS(O)2NR r R r, -NR r S(O)2NR r R r, -NHR
r, -NR r R r, cycloalkylamino, and -R x;

R r, R s, and R t at each occurrence are independently selected from the group consisting of C1-C6 alkyl, C3-6 alkenyl, C3-6 alkynyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl; wherein C1-C6 alkyl is optionally substituted with one or more substituents selected from the group consisting of-R y, fluoro, -OH, -NH2, C1-alkoxy, fluoro substituted C1-C6 alkoxy, C1-C6 alkylthio, fluoro substituted alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the C1-C6 alkyl carbon bound to any O, S, or N, of -OR r, -SR r, -C(O)OR r, -C(S)OR r, -C(O)NHR r, -C(S)NHR r, -C(O)NR r R r, -C(S)NR r R r, -S(O)2NHR r, -S(O)2NR r R r, -C(NH)NHR r, -NR r C(O)R r, -NR r C(S)R r, -NR r S(O)2R r, -NHC(O)NHR r, -NHC(S)NHR r, -NR r C(O)NH2, -NR r C(S)NH2, -NR r C(O)NHR r, -NR r C(S)NHR r, -NHC(O)NR r R r, -NHC(S)NR r R r, -NR r C(O)NR r R r, -NR r C(S)NR r R r, -NHS(O)2NHR r, -NR r S(O)2NH2, -NR r S(O)2NHR r, -NHS(O))2NR r R r, -NR r S(O))2NR r R r, -NHR r, or -NR r R r is selected from the group consisting of fluoro and -R y; and wherein C3-6 alkenyl or C3-6 alkynyl, respectively, are optionally substituted with one or more substituents selected from the group consisting of -R y, fluoro, C1-C6 alkyl, fluoro substituted C1-C6 alkyl, C1-C6 alkoxy, fluoro substituted C1-C6 alkoxy, C1-C6 alkylthio, fluoro substituted C1-C6 alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the C3-6 alkenyl or C3-6 alkynyl carbon bound to any O, S, or N, of -OR r, -SR r, -C(O)OR r, -C(S)OR r, -C(O)NHR r, -C(S)NHR r, -C(O)NR r R r, -C(S)NR r R r, -S(O)2NHR r, -S(O)2NR r R r, -C(NH)NHR r, -NR r C(O)R r, -NR r C(S)R r, -NR r S(O)2R r, -NHC(O)NHR r, -NHC(S)NHR r, -NR r C(O)NH2, -NR r C(S)NH2, -NR r C(O)NHR r, -NR r C(S)NHR r, -NHC(O)NR r R r, -NHC(S)NR r R r, -NR r C(O)NR r R r, -NR r C(S)NR r R r, -NHS(O)2NHR r, -NR r S(O)2NH2, -NR r S(O)2NHR r, -NHS(O)2NR r R r, -NR r S(O)2NR r R r, -NHR r, or -NR r R r is selected from the group consisting of fluoro, C1-C6 alkyl, fluoro substituted C1-C6 alkyl, and -R y; and wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, respectively, are optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, C1-C6 alkyl, fluoro substituted C1-C6 alkyl, C1-C6 alkoxy, fluoro substituted C1-C6 alkoxy, C1-C6 alkylthio, fluoro substituted C1-C6 alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, or R s and R t combine with the nitrogen to which they are attached form a 5-7 membered heterocycloalkyl or a 5 or 7 membered nitrogen containing heteroaryl, wherein the 5-7 membered heterocycloalkyl or 5 or 7 membered nitrogen containing heteroaryl, respectively, are optionally substituted with one or more substituents selected from the group consisting of halogen, -NO2, -CN, -OH, -NH2, OR u, -SR u, -NHR u, -NR u R u, -R x, and -R y;
each R u is independently selected from the group consisting of C1-C6 alkyl, alkenyl, C3-6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein C1-C6 alkyl is optionally substituted with one or more substituents selected from the group consisting of -R y, fluoro, -OH, -NH2, C1-C6 alkoxy, fluoro substituted C1-C6 alkoxy, C1-C6 alkylthio, fluoro substituted C1-C6 alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the C1-C6 alkyl carbon bound to the O of -OR u, S of -SR u, or N of -NHR u is fluoro or -R y;
and wherein C3-6 alkenyl or C3-6 alkynyl, respectively, are optionally substituted with one or more substituents selected from the group consisting of -R y, fluoro, -OH, -NH2, C1-C6 alkyl, fluoro substituted CI-C6 alkyl, C1-C6 alkoxy, fluoro substituted C1-C6 alkoxy, alkylthio, fluoro substituted C1-C6 alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino, provided, however, that any substitution of the C3-6 alkenyl or C3-6 alkynyl carbon bound to the O of -OR u, S of -SR u, or N of -NHR u is fluoro, alkyl, fluoro substituted C1-C6 alkyl, or -R y; and wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, respectively, are optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, C1-C6 alkyl, fluoro substituted C1-C6 alkyl, C1-C6 alkoxy, fluoro substituted alkoxy, C1-C6 alkylthio, fluoro substituted C1-C6 alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;

each R x is selected from the group consisting of C1-C6 alkyl, C2-C6 alkenyl and C2-C6 alkynyl, wherein C1-C6 alkyl is optionally substituted with one or more substituents selected from the group consisting of -R y, fluoro, -OH, -NH2, C1-alkoxy, fluoro substituted C1-C6 alkoxy, C1-C6 alkylthio, fluoro substituted alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino; and wherein C2-alkenyl or C2-C6 alkynyl, respectively, are optionally substituted with one or more substituents selected from the group consisting of -R y, fluoro, -OH, -NH2, C1-C6 alkyl, fluoro substituted C1-C6 alkyl, C1-C6 alkoxy, fluoro substituted C1-C6 alkoxy, alkylthio, fluoro substituted C1-C6 alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;

each R y is selected from the group consisting of cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl, and heteroaryl, respectively, are optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -NH2, -NO2, -CN, C1-C6 alkyl, fluoro substituted C1-C6 alkyl, C1-C6 alkoxy, fluoro substituted C1-C6 alkoxy, C1-C6 alkylthio, fluoro substituted C1-C6 alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino;
at each occurrence, alkenyl, by itself or as part of another substituent, is a straight or branched chain hydrocarbon having at least one carbon-to-carbon double bond;
at each occurrence, alkynyl, by itself or as part of another substituent, is a straight or branched chain hydrocarbon having at least one carbon-to-carbon triple bond;

at each occurrence, cycloalkyl, by itself or as part of another substituent, is a saturated or unsaturated, non-aromatic monocyclic, bicyclic or tricyclic carbon ring system of 3-10 ring members per ring; and at each occurrence, heterocycloalkyl, by itself or as part of another substituent, is a saturated or unsaturated non-aromatic group having from 5 to 10 atoms in which from 1 to 3 carbon atoms in the ring are replaced by heteroatoms of O, S or N, and are optionally fused with benzo or heteroaryl of 5-6 ring members.
2. The compound of claim 1, wherein:
R81 is selected from the group consisting of hydrogen, halogen, optionally substituted C1-C6 alkyl, optionally substituted C2-C6 alkenyl, optionally substituted C2-C6 alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, -CN, -S(O)2NH2, -C(O)NH2, -OR68, -SR68, -NR69R68, -C(O)R68, -C(S)R68, -C(O)NR
69R68, -S(O)2NR69R68, -NR69C(O)R68, -NR69S(O)2R68, -S(O)R68, and -S(O)2R68.
3. The compound of claim 1, wherein:
C1-C6 alkyl as R68, R69, R79, R80, or R81, C2-6 alkyl as R112, C2-C6 alkenyl as R68 or R81, or C2-C6 alkynyl as R68 or R81 are optionally substituted with 1, 2, or 3 groups or substituents selected from the group consisting of fluoro, -NO2, -CN, -OR1a, -SR1a, -NR1aR1a, -OC(O)R1a, -OC(S)R1a, -C(O)R1a, -C(S)R1a, -C(O)OR1a, -C(S)OR1a, -C(O)NR1a R1a, -C(S)NR1a R1a, -S(O)2NR1a R1a, -C(NH)NR1a R1a, -NR1a C(O)R1a, -NR1a C(S)R1a, -NR1a S(O)2R1a, -NR1a C(O)NR1a R1a, -NR1a C(S)NR1a R1a, -NR1a S(O)2NR1a R1a, -S(O)R1a, -S(O)2R1a, cycloalkyl, heterocycloalkyl, aryl and heteroaryl;
cycloalkyl, heterocycloalkyl, aryl or heteroaryl, as R68, R81, R112, or a substituent of C1-C6 alkyl, C2-C6 alkenyl or C2-C6 alkynyl; or 5-7 membered heterocycloalkyl as R79 and R80 combined with the nitrogen to which they are attached, are optionally substituted with 1, 2, or 3 groups or substituents selected from the group consisting of halogen, -NO2, -CN, -OR1a, -SR1a, -NR1a R1a, -OC(O)R1a, -OC(S)R1a, -C(O)R1a, -C(S)R1a, -C(O)OR1a, -C(S)OR1a, -C(O)NR1a R1a, -C(S)NR1a R1a, -S(O)2NR1a R1a, -C(NH)NR1a R1a, -NR1a C(O)R1a, -NR1a C(S)R1a, -NR1 aS(O)2R1a, -NR1a C(O)NR1a R1a, -NR1a C(S)NR1a R1a, -NR1a S(O)2NR1a R1a, -S(O)R1a, -S(O)2R1a, -R1b, and C1-C6 alkyl, wherein C1-C6 alkyl is optionally substituted with 1, 2 or 3 groups or substituents selected from the group consisting of fluoro, -OH, -NH2, C1-C6 alkoxy, fluoro substituted C1-C6 alkoxy, C1-C6 alkylthio, fluoro substituted C1-C6 alkylthio, mono-alkylamino, di-alkylamino, and -R1b;
R1a is selected from the group consisting of hydrogen, -R1b, and C1-C6 alkyl, provided, however, that hydrogen is not bound to any of C(S), C(O), S(O), or S(O)2 of -OC(O)R1a, -OC(S)R1a, -C(O)R1a, -C(S)R1a, -NR1a C(O)R1a, -NR1a C(S)R1a, -NR1a S(O)2R1a, -S(O)R1a, or -S(O)2R1a, wherein C1-C6 alkyl is optionally substituted with 1, 2 or 3 groups or substituents selected from the group consisting of fluoro, -OH, -NH2, C1-C6 alkoxy, fluoro substituted C1-C6 alkoxy, C1-C6 alkylthio, fluoro substituted C1-C6 alkylthio, mono-alkylamino, di-alkylamino, and -R1b, provided, however, that any substitution of the alkyl carbon bound to O, S, or N of -OR1a, -SR1a, -NR1a R1a, -C(O)OR1a, -C(S)OR1a, -C(O)NR1a R1a, -C(S)NR1a R1a, -S(O)2NR1a R1a, -C(NH)NR1a R1a, -NR1a C(O)R1a, -NR1a C(S)R1a, -NR1a S(O)2R1a, -NR1a C(O)NR1a R1a, -NR1a C(S)NR1a R1a, or -NR1a S(O)2NR1a R1a, is fluoro or -R1b; and R1b is selected from the group consisting of cycloalkyl, heterocycloalkyl, aryl and heteroaryl, wherein cycloalkyl, heterocycloalkyl, aryl and heteroaryl are optionally substituted with 1, 2 or 3 groups or substituents selected from the group consisting of halogen, -CN, -OH, -NH2, C1-C6 alkoxy, fluoro substituted C1-C6 alkoxy, C1-C6 alkylthio, fluoro substituted C1-C6 alkylthio, mono-alkylamino, di-alkylamino, and cycloalkylamino.
4. The compound of claim 1, wherein:
C1-C6 alkyl as R68, R69, R79, R80, or R81, C2-6 alkyl as R112, C2-C6 alkenyl as R68 or R81, or C2-C6 alkynyl as R68 or R81 are optionally substituted with 1, 2, or 3 subtituents selected from the group consisting of fluoro, -CN, -OR1a, -SR1a, -NR1a R1a, -C(O)R1a, -C(S)R1a, -C(O)OR1a, -C(O)NR1a R1a, -C(S)NR1a R1a, -S(O)2NR1a R1a, -NR1a C(O)R1a, -NR1a C(S)R1a, -NR1a S(O)2R1a, -S(O)R1a, -S(O)2R1a, cycloalkyl, heterocycloalkyl, aryl and heteroaryl; and cycloalkyl, heterocycloalkyl, aryl or heteroayl, as R68, R81, R112, or a substituent of C1-C6 alkyl, C2-C6 alkenyl or C2-C6 alkynyl; or 5-7 membered heterocycloalkyl as R79 and R80 combined with the nitrogen to which they are attached, are optionally substituted with 1, 2, or 3 substituents selected from the group consisting of halogen, -CN, -OR1a, -SR1a, -NR1a R1a, -C(O)R1a, -C(S)R1a, -C(O)OR1a, -C(O)NR1a R1a, -C(S)NR1a R1a, -S(O)2NR1a R1a, -NR1a C(O)R1a, -NR1a C(S)R1a, -NR1a S(O)2R1a, -S(O)R1a, -S(O)2R1a, -R1b, and C1-C6 alkyl, wherein C1-C6 alkyl is optionally substituted with 1, 2 or 3 substituents selected from the group consisting of fluoro, -OH, -NH2, C1-alkoxy, fluoro substituted C1-C6 alkoxy, C1-C6 alkylthio, fluoro substituted alkylthio, mono-alkylamino, di-alkylamino, and -R1b.
5. The compound of claim 1, wherein R112 is optionally substituted C2-6 alkyl.
6. The compound of claim 1, wherein R112 is -NR79R80.
7. The compound of claim 1, wherein R112 is optionally substituted aryl.
8. The compound of claim 1, wherein R112 is optionally substituted heteroaryl.
9. The compound of claim 1, wherein:
R81 is selected from the group consisting of hydrogen; halogen; C1-6 alkyl optionally substituted with carboxylic acid; C2-6 alkenyl optionally substituted with carboxylic acid; C1-6 alkoxy optionally substituted with methoxy or diethylamine;
carboxylic acid; carboxylic acid methyl ester; carboxylic acid ethylamide;
4-methyl-piperidin-1-yl; 4-methyl-piperazin-1-yl; morpholin-4-yl; phenyl-amino;
phenyl optionally substituted with halogen, -CN, optionally fluoro substituted alkyl, dimethylamine, methoxy, carboxylic acid, carboxylic acid amide, carboxylic acid-dimethyl amide, morpholine-4-carbonyl, morpholine, morpholine-4-methyl, or 2-methoxy-ethoxy; pyridinyl optionally substituted with methoxy, morpholine, or 4-methyl-piperazin-1-yl; 4-methyl-1H-imidazol-2-yl; and N-methyl-pyrazolyl;
R83 is selected from the group consisting of hydrogen, fluoro and chloro;
R112 is selected from the group consisting of C2-6 alkyl; phenyl optionally substituted with -CN, -NO2, acetamide, halogen, optionally fluoro substituted alkyl, optionally fluoro substituted C1-6 alkoxy, or oxazolyl; 2,3-dihydro-benzo[1,4]dioxin-6-yl; methyl substituted thiazole, methyl substituted imidazole, thiophene optionally substituted with methyl, oxazole, isoxazole, or pyridine;
furan substituted with methyl or carboxylic acid methyl ester; benzothiazol-6-yl;
benzo[b]thiophen-2-yl; piperidin-1-yl; and dimethylamine.
10. The compound of claim 9, wherein R112 is C2-6 alkyl.
11. The compound of claim 9, wherein R112 is piperidin-1-yl or dimethylamine.
12. The compound of claim 9, wherein R112 is selected from the group consisting of 2,3-dihydro-benzo[1,4]dioxin-6-yl; and phenyl optionally substituted with -CN, -NO2, acetamide, halogen, optionally fluoro substituted C1-6 alkyl, optionally fluoro substituted C1-6 alkoxy, or oxazolyl.
13. The compound of claim 9, wherein R112 is selected from the group consisting of methyl substituted thiazole; methyl substituted imidazole; thiophene optionally substituted with methyl, oxazole, isoxazole, or pyridine; furan substituted with methyl or carboxylic acid methyl ester; benzothiazol-6-yl; and benzo[b]thiophen-2-yl.
14. The compound of claim 1, wherein the compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
15. The compound of claim 1, wherein the compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
16. The compound of claim 1, wherein the compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
17. The compound of claim 1, wherein the compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
18. The compound of claim 1, wherein the compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
19. The compound of claim 1, wherein the compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
20. The compound of claim 1, wherein the compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
21. The compound of claim 1, wherein the compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
22. The compound of claim 1, wherein the compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
23. The compound of claim 1, wherein the compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
24. The compound of claim 1, wherein the compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
25. The compound of claim 1, wherein the compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
26. The compound of claim 1, wherein said compound is selected from the group consisting of:

and pharmaceutically acceptable salts thereof.
27. The compound of claim 1, wherein the compound is N-[3-(5-chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-2,4-difluoro-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
28. The compound of claim 1, wherein the compound is N-[2,4-difluoro-3-(5-methoxy-1H-pyrrolo[2,3 -b]pyridine-3-carbonyl)-phenyl]-3-trifluoromethyl-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
29. The compound of claim 1, wherein the compound is N-[3-(5-chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-3-trifluoromethyl-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
30. The compound of claim 1, wherein the compound is N-[2,4-difluoro-3-(5-methoxy-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-phenyl]-4-trifluoromethyl-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
31. The compound of claim 1, wherein the compound is is N-{2,4-difluoro-3-[5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrrolo[2,3-b]pyridine-3-carbonyl]-phenyl}-3-fluoro-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
32. The compound of claim 1, wherein the compound is N-[3-(5-chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-2-cyano-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
33. The compound of claim 1, wherein the compound is N-{2,4-difluoro-3-[5-(6-methoxy-pyridin-3-yl)-1H-pyrrolo[2,3-b]pyridine-3-carbonyl]-phenyl}-3-fluoro-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
34. The compound of claim 1, wherein the compound is N-[3-(5-chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-4-trifluoromethyl-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
35. The compound of claim 1, wherein the compound is N-[3-(5-chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-3-cyano-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
36. The compound of claim 1, wherein the compound is N-{2,4-difluoro-3-[5-(2-methoxy-ethoxy)-1H-pyrrolo[2,3-b]pyridine-3-carbonyl]-phenyl}-3-fluoro-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
37. The compound of claim 1, wherein the compound is N-[3-(5-chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-4-isopropyl-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
38. The compound of claim 1, wherein the compound is N-[3-(5-chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-4-ethyl-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
39. The compound of claim 1, wherein the compound is N-[2,4-difluoro-3-(1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-phenyl]-2,5-dimethoxy-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
40. The compound of claim 1, wherein the compound is N-[3-(5-chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-4-difluoromethoxy-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
41. The compound of claim 1, wherein the compound is 4-butoxy-N-[2,4-difluoro-3-(1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-phenyl]-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
42. The compound of claim 1, wherein the compound is N-[3-(5-chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-4-cyano-benzenesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
43. The compound of claim 1, wherein the compound is dimethylamine-1-sulfonic acid [3-(5-pyridin-3-yl-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-amide having the structure:

or a pharmaceutically acceptable salt thereof.
44. The compound of claim 1, wherein the compound is butane-1-sulfonic acid [3-(5-chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2-fluoro-phenyl]-amide having the structure:

or a pharmaceutically acceptable salt thereof.
45. The compound of claim 1, wherein the compound is N-[2,4-difluoro-3-(5-pyridin-3-yl-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-phenyl]-ethanesulfonamide having the structure:

or a pharmaceutically acceptable salt thereof.
46. The compound of claim 1, wherein the compound is propane-1-sulfonic acid [2,4-difluoro-3-(5-pyridin-3-yl-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-phenyl]-amide having the structure:

or a pharmaceutically acceptable salt thereof.
47. The compound of claim 1, wherein the compound is propane-1-sulfonic acid [2,4-difluoro-3-(5-morpholin-4-yl-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-phenyl]-amide having the structure:

or a pharmaceutically acceptable salt thereof.
48. The compound of claim 1, wherein the compound is propane-1-sulfonic acid {3-[5-(4-chloro-phenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonyl]-2,4-difluoro-phenyl}-amide having the structure:

or a pharmaceutically acceptable salt thereof.
49. The compound of claim 1, wherein the compound is propane-1-sulfonic acid {2,4-difluoro-3-[5-(4-trifluoromethyl-phenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonyl]-phenyl}-amide having the structure:

or a pharmaceutically acceptable salt thereof.
50. The compound of claim 1, wherein the compound is propane-1-sulfonic acid [3-(5-chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluoro-phenyl]-amide having the structure:

or a pharmaceutically acceptable salt thereof.
51. The compound of claim 1, wherein the compound is propane-1-sulfonic acid {3-[5-(4-chloro-phenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonyl]-2-fluoro-phenyl}-amide having the structure:

or a pharmaceutically acceptable salt thereof.
52. The compound of claim 1, wherein the compound is propane-1-sulfonic acid [3-(5-chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2-fluoro-phenyl]-amide having the structure:

or a pharmaceutically acceptable salt thereof.
53. The compound of claim 1, wherein the compound is propane-1-sulfonic acid [4-chloro-2-fluoro-3-(5-pyridin-3-yl-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-phenyl]-amide having the structure:

or a pharmaceutically acceptable salt thereof.
54. The compound of claim 1, wherein the compound is propane-1-sulfonic acid [2-fluoro-3-(5-fluoro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-phenyl]-amide having the structure:

or a pharmaceutically acceptable salt thereof.
55. The compound of claim 1, wherein the compound is propane-1-sulfonic acid {2,4-difluoro-3-[5-(2-methoxy-ethoxy)-1H-pyrrolo[2,3-b]pyridine-3-carbonyl]-phenyl}-amide having the structure:

or a pharmaceutically acceptable salt thereof.
56. A composition comprising: a pharmaceutically acceptable carrier; and a compound according to any one of Claims 1-55.
57. A kit comprising a compound according to any one of Claims 1-55 or a composition according to Claim 56, together with instructions for its use for the treatment of melanoma, glioma, thyroid cancer, liver cancer, lung cancer, colon cancer, acute pain, chronic pain, or polycystic kidney disease.
58. A compound according to any one of Claims 1-55 for use in the treatment of melanoma, glioma, thyroid cancer, liver cancer, lung cancer, colon cancer, acute pain, chronic pain, or polycystic kidney disease.
59. Use of a compound according to any one of Claims 1-55 or a composition according to Claim 56 in the preparation of a medicament for the treatment of a disease or condition selected from the group consisting of ischemic stroke, cerebrovascular ischemia, multi-infarct dementia, head injury, spinal cord injury, Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, dementia, senile chorea, Huntington's disease, chemotherapy-induced hypoxia, gastrointestinal stromal tumors, prostate tumors, mast cell tumors, canine mast cell tumors, acute myeloid leukemia, acute lymphocytic leukemia, chronic myeloid leukemia, multiple myeloma, melanoma, mastocytosis, gliomas, glioblastoma, astrocytoma, neuroblastoma, sarcomas, lung carcinomas, breast carcinomas, pancreatic carcinomas, renal carcinomas, female genital tract carcinomas, carcinoma in situ, lymphoma, histiocytic lymphoma, neurofibromatosis, Schwann cell neoplasia, myelodysplastic syndrome, leukemia, tumor angiogenesis, cancers of the thyroid, liver, bone, skin, brain, pancreas, lung, breast, colon, prostate, testes and ovary, small cell lung cancer, pain of neuropathic or inflammatory origin, acute pain, chronic pain, migraine, heart failure, cardiac hypertrophy, thrombosis, thrombotic microangiopathy syndromes, atherosclerosis, reperfusion injury, ischemia cerebrovascular ischemia, liver ischemia, inflammation, polycystic kidney disease, age-related macular degeneration, rheumatoid arthritis, allergic rhinitis, inflammatory bowel disease, ulcerative colitis, Crohn's disease, systemic lupus erythematosis, Sjogren's Syndrome, Wegener's granulomatosis, psoriasis, scleroderma, chronic thyroiditis, Grave's disease, myasthenia gravis, multiple sclerosis, osteoarthritis, endometriosis, scarring, vascular restenosis, fibrotic disorders, hypereosinophilia, CNS inflammation, pancreatitis, nephritis, atopic dermatitis, hepatitis, immunodeficiency diseases, organ transplant rejection, graft versus host disease, diabetic nephropathy, nephrosclerosis, glomerulonephritis, interstitial nephritis, Lupus nephritis, prostate hyperplasia, chronic renal failure, tubular necrosis, diabetes-associated renal complications, hypertrophy, type 1 diabetes, type 2 diabetes, metabolic syndrome, obesity, hepatic steatosis, insulin resistance, hyperglycemia, lipolysis, obesity, infection, Helicobacter pylori infection, Influenza virus infection, fever, sepsis, chronic obstructive pulmonary disease, acute respiratory distress syndrome, asthma, allergy, bronchitis, emphysema, pulmonary fibrosis, Noonan's syndrome, Costello syndrome, leopard syndrome, cardio-faciocutaneous syndrome, neural crest syndrome abnormalities causing cardiovascular, skeletal, intestinal, skin, hair or endocrine diseases, osteoporosis, increased risk of fracture, hypercalcemia, and bone metastases.
60. The use according to Claim 59, wherein said disease or condition is selected from the group consisting of melanoma, glioma, thyroid cancer, liver cancer, lung cancer, colon cancer, acute pain, chronic pain, and polycystic kidney disease.
61. Use of a compound according to any one of Claims 5, 6, 10, 11, 14-19 or 43-55 in the preparation of a pharmaceutical for the treatment of melanoma.
62. Use of a compound according to any one of Claims 5, 6, 10, 11, 14-19 or 43-55 in the preparation of a pharmaceutical for the treatment of thyroid cancer.
63. Use of a compound according to any one of Claims 5, 6, 10, 11, 14-19 or 43-55 in the preparation of a pharmaceutical for the treatment of colorectal cancer.
64. Use of a compound according to any one of Claims 5, 6, 10, 11, 14-19 or 43-55 in the preparation of a pharmaceutical for the treatment of lung cancer.
65. Use of a compound according to any one of Claims 5, 6, 10, 11, 14-19 or 43-55 in the preparation of a pharmaceutical for the treatment of prostate cancer.
66. Use of a compound according to any one of Claims 5, 6, 10, 11, 14-19 or 43-55 in the preparation of a pharmaceutical for the treatment of liver cancer.
67. Use of a compound according to any one of Claims 5, 6, 10, 11, 14-19 or 43-55 in the preparation of a pharmaceutical for the treatment of glioma.
68. Use of a compound according to any one of Claims 20, 30, 34, 37, 38 or 40-42 in the preparation of a pharmaceutical for the treatment of polycystic kidney disease.
69. Use of a compound according to any one of Claims 20, 30, 34, 37, 38 or 40-42 in the preparation of a pharmaceutical for the treatment of acute pain.
70. Use of a compound according to any one of Claims 20, 30, 34, 37, 38 or 40-42 in the preparation of a pharmaceutical for the treatment of chronic pain.
CA2613015A 2005-06-22 2006-06-21 Pyrrolo [2, 3-b] pyridine derivatives as protein kinase inhibitors Active CA2613015C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US69296005P 2005-06-22 2005-06-22
US60/692,960 2005-06-22
US73152805P 2005-10-28 2005-10-28
US60/731,528 2005-10-28
PCT/US2006/024524 WO2007002433A1 (en) 2005-06-22 2006-06-21 Pyrrolo [2, 3-b] pyridine derivatives as protein kinase inhibitors

Publications (2)

Publication Number Publication Date
CA2613015A1 CA2613015A1 (en) 2007-01-04
CA2613015C true CA2613015C (en) 2012-04-03

Family

ID=37033831

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2613015A Active CA2613015C (en) 2005-06-22 2006-06-21 Pyrrolo [2, 3-b] pyridine derivatives as protein kinase inhibitors

Country Status (34)

Country Link
US (10) US7863288B2 (en)
EP (3) EP3088400A1 (en)
JP (1) JP5007304B2 (en)
KR (1) KR101125919B1 (en)
CN (3) CN101243084B (en)
AR (2) AR054624A1 (en)
AT (1) ATE518860T1 (en)
AU (1) AU2006261993B2 (en)
CA (1) CA2613015C (en)
CR (2) CR9677A (en)
CY (2) CY1111996T1 (en)
DE (1) DE122012000043I1 (en)
DK (2) DK2395004T3 (en)
EC (1) ECSP088121A (en)
ES (2) ES2371397T3 (en)
HK (2) HK1114610A1 (en)
HU (2) HUE027370T2 (en)
IL (1) IL188248A (en)
LU (1) LU92035I2 (en)
MA (1) MA29612B1 (en)
MX (1) MX2007016463A (en)
MY (2) MY147410A (en)
NO (2) NO333913B1 (en)
NZ (1) NZ565255A (en)
PE (1) PE20070100A1 (en)
PL (2) PL2395004T3 (en)
PT (1) PT1893612E (en)
RS (1) RS52010B (en)
RU (3) RU2565071C2 (en)
SI (2) SI2395004T1 (en)
TW (3) TWI432193B (en)
UA (1) UA95244C2 (en)
WO (2) WO2007002433A1 (en)
ZA (4) ZA200711152B (en)

Families Citing this family (406)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI1696920T1 (en) 2003-12-19 2015-02-27 Plexxikon Inc. Compounds and methods for development of ret modulators
RS51830B (en) 2004-03-30 2012-02-29 Vertex Pharmaceuticals Incorporated Azaindoles useful as inhibitors of jak and other protein kinases
US7498342B2 (en) 2004-06-17 2009-03-03 Plexxikon, Inc. Compounds modulating c-kit activity
JP4834553B2 (en) 2004-09-17 2011-12-14 エーザイ・アール・アンド・ディー・マネジメント株式会社 Pharmaceutical composition
KR20080027775A (en) 2005-05-17 2008-03-28 플렉시콘, 인코퍼레이티드 Pyrrole(2,3-b)pyridine derivatives protein kinase inhibitors
US7863288B2 (en) * 2005-06-22 2011-01-04 Plexxikon, Inc. Compounds and methods for kinase modulation, and indications therefor
EP2251341A1 (en) 2005-07-14 2010-11-17 Astellas Pharma Inc. Heterocyclic Janus kinase 3 inhibitors
EP1910358A2 (en) 2005-07-14 2008-04-16 Astellas Pharma Inc. Heterocyclic janus kinase 3 inhibitors
EP2281901B1 (en) 2005-08-02 2013-11-27 Eisai R&D Management Co., Ltd. Anti-tumour pharmaceutical composition with angiogenesis inhibitors
CA2620594C (en) * 2005-09-01 2012-08-21 Eisai R&D Management Co., Ltd. Pharmaceutical composition having improved disintegratability
AU2006326548B2 (en) 2005-12-13 2012-04-05 Incyte Holdings Corporation Heteroaryl substituted pyrrolo[2,3-b]pyridines and pyrrolo[2,3-b]pyrimidines as Janus kinase inhibitors
CN104650077A (en) 2006-01-17 2015-05-27 沃泰克斯药物股份有限公司 Azaindoles useful as inhibitors of janus kinases
WO2007084667A2 (en) * 2006-01-19 2007-07-26 Osi Pharmaceutical, Inc. Fused heterobicyclic kinase inhibitors
TWI382984B (en) 2006-04-03 2013-01-21 Astellas Pharma Inc Hetero compound
EP2036557B1 (en) 2006-05-18 2015-10-21 Eisai R&D Management Co., Ltd. Antitumor agent for thyroid cancer
EP2029593A1 (en) * 2006-05-22 2009-03-04 AstraZeneca AB Indole derivatives
US7964728B2 (en) 2006-07-06 2011-06-21 Solvay Pharmaceuticals B.V. Azaindole derivatives with a combination of partial nicotinic acetyl-choline receptor agonism and dopamine reuptake inhibition
EA015700B1 (en) * 2006-07-06 2011-10-31 Солвей Фармасьютикалс Б.В. Azaindole derivatives with a combination of partial nicotinic acetylcholine receptor agonism and dopamine reuptake inhibition
GB0617161D0 (en) * 2006-08-31 2006-10-11 Vernalis R&D Ltd Enzyme inhibitors
CN101610676B (en) 2006-09-22 2013-03-27 药品循环公司 Inhibitors of bruton's tyrosine kinase
WO2008063888A2 (en) 2006-11-22 2008-05-29 Plexxikon, Inc. Compounds modulating c-fms and/or c-kit activity and uses therefor
EP2124951B1 (en) 2006-12-21 2014-05-21 Vertex Pharmaceuticals Inc. 5-cyan0-4- (pyrrolo[2, 3b]pyridine-3-yl) -pyrimidine derivatives useful as protein kinase inhibitors
WO2008079909A1 (en) * 2006-12-21 2008-07-03 Plexxikon, Inc. Pyrrolo [2,3-b] pyridines as kinase modulators
WO2008080015A2 (en) * 2006-12-21 2008-07-03 Plexxikon, Inc. Compounds and methods for kinase modulation, and indications therefor
PE20081581A1 (en) * 2006-12-21 2008-11-12 Plexxikon Inc PIRROLO [2,3-b] PYRIDINES COMPOUNDS AS KINASE MODULATORS
US20120101114A1 (en) 2007-03-28 2012-04-26 Pharmacyclics, Inc. Inhibitors of bruton's tyrosine kinase
KR101425248B1 (en) * 2007-03-28 2014-08-06 파마시클릭스, 인코포레이티드 Inhibitors of bruton's tyrosine kinase
EP2851091B1 (en) 2007-04-13 2017-12-27 Dana-Farber Cancer Institute, Inc. Methods for treating cancer resistant to ERBB therapeutics
GB0710528D0 (en) * 2007-06-01 2007-07-11 Glaxo Group Ltd Novel compounds
EP2173752B2 (en) 2007-06-13 2022-07-13 Incyte Holdings Corporation Salts of the janus kinase inhibitor (r)-3-(4-(7h-pyrrolo(2,3-d)pyrimidin-4-yl)-1h-pyrazol-1-yl)-3-cyclopentylpropanenitrile
AU2008276063B2 (en) * 2007-07-17 2013-11-28 Plexxikon Inc. Compounds and methods for kinase modulation, and indications therefor
MX2010001636A (en) * 2007-08-14 2010-03-15 Hoffmann La Roche Diazo bicyclic smac mimetics and the uses thereof.
US8476273B2 (en) 2007-08-17 2013-07-02 University Of Maryland, Baltimore Small molecule inhibitors of Lck SH2 domain binding
JP2009077712A (en) 2007-09-11 2009-04-16 F Hoffmann La Roche Ag DIAGNOSTIC TEST FOR SUSCEPTIBILITY TO B-Raf KINASE INHIBITOR
DE102007045919B4 (en) 2007-09-26 2018-07-05 Bayer Intellectual Property Gmbh Drug combinations with insecticidal and acaricidal properties
DE102007045956A1 (en) 2007-09-26 2009-04-09 Bayer Cropscience Ag Combination of active ingredients with insecticidal and acaricidal properties
JP2011500806A (en) 2007-10-25 2011-01-06 メルク・シャープ・エンド・ドーム・コーポレイション Therapeutic compounds
US8426396B2 (en) * 2008-01-08 2013-04-23 Shriners Hospitals For Children Treatment for achondroplasia
WO2009089352A1 (en) 2008-01-08 2009-07-16 Array Biopharma Inc. Pyrrolopyridines as kinase inhibitors
JP5608098B2 (en) 2008-01-09 2014-10-15 アレイ バイオファーマ、インコーポレイテッド Pyrazolopyridines as kinase inhibitors
JP2011510004A (en) * 2008-01-22 2011-03-31 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング Protein kinase inhibitors and uses thereof
WO2009106445A1 (en) * 2008-02-25 2009-09-03 F. Hoffmann-La Roche Ag Pyrrolopyrazine kinase inhibitors
PE20091623A1 (en) 2008-02-29 2009-11-19 Array Biopharma Inc 1H-PYRAZOLO [3,4-b] PYRIDINE DERIVATIVES AS RAF KINASE INHIBITORS
JP2011513332A (en) 2008-02-29 2011-04-28 アレイ バイオファーマ、インコーポレイテッド N- (6-Aminopyridin-3-yl) -3- (sulfonamido) benzamide derivatives as RAF inhibitors for the treatment of cancer
AR070535A1 (en) 2008-02-29 2010-04-14 Array Biopharma Inc RAF INHIBITING COMPOUNDS AND METHODS FOR USE
UA103319C2 (en) 2008-05-06 2013-10-10 Глаксосмитклайн Ллк Thiazole- and oxazole-benzene sulfonamide compounds
US8158636B2 (en) 2008-05-19 2012-04-17 Plexxikon Inc. Compounds and methods for kinase modulation, and indications therefor
PE20091846A1 (en) 2008-05-19 2009-12-16 Plexxikon Inc PIRROLO [2,3-d] -PYRIMIDINE DERIVATIVES AS KINE MODULATORS
CN102036982A (en) * 2008-05-21 2011-04-27 巴斯夫欧洲公司 Substituted pyridin-4-yl-methyl sulfonamides as fungicides
BRPI0912109A2 (en) * 2008-05-28 2015-07-28 Basf Se Compost, process for preparing same, agrochemical compositions, method for combating phytopathogenic harmful fungi, use of the compounds, and seed
WO2009152087A1 (en) 2008-06-10 2009-12-17 Plexxikon, Inc. Bicyclic heteroaryl compounds and methods for kinase modulation, and indications therefor
EP2313411A1 (en) * 2008-06-10 2011-04-27 Plexxikon, Inc. 5h-pyrr0l0 [2,3-b]pyrazine derivatives for kinase modulation, and indications therefor
AR072008A1 (en) * 2008-06-13 2010-07-28 Merck & Co Inc HETEROBICICLIC COMPOUNDS AS QUINASA P38 INHIBITION AGENTS
US9447049B2 (en) 2010-03-01 2016-09-20 University Of Tennessee Research Foundation Compounds for treatment of cancer
US20110130384A1 (en) 2008-06-25 2011-06-02 Takeda Pharmaceutical Company Limited Amide compound
WO2010009342A2 (en) 2008-07-16 2010-01-21 Pharmacyclics, Inc. Inhibitors of bruton's tyrosine kinase for the treatment of solid tumors
DE102008052943A1 (en) 2008-10-23 2010-04-29 Merck Patent Gmbh azaindole derivatives
EP2356116A1 (en) * 2008-11-20 2011-08-17 OSI Pharmaceuticals, Inc. Substituted pyrroloý2,3-b¨-pyridines and-pyrazines
WO2010068483A2 (en) * 2008-11-25 2010-06-17 University Of Rochester Mlk inhibitors and methods of use
WO2010065927A2 (en) * 2008-12-05 2010-06-10 California Institute Of Technology Dna-damage-induced proteolysis
BRPI1008326A2 (en) * 2009-02-10 2015-08-25 Divergence Inc Compositions and methods for controlling nanatodes
KR101663339B1 (en) * 2009-03-11 2016-10-06 플렉시콘 인코퍼레이티드 Pyrrolo[2,3-b]pyridine derivatives for the inhibition of raf kinases
CA2755197A1 (en) * 2009-03-11 2010-09-16 Plexxikon, Inc. Pyrrolo [2, 3-b] pyridine derivatives for the inhibition of raf kinases
WO2010111527A1 (en) * 2009-03-26 2010-09-30 Plexxikon, Inc. Pyrazolo [ 3, 4 -b] pyridines as kinase inhibitors and their medical use
MX2011008303A (en) * 2009-04-03 2011-11-29 Plexxikon Inc Propane- i-sulfonic acid {3- [5- (4 -chloro-phenyl) -1h-pyrrolo [2, 3-b] pyridine-3-carbonyl] -2, 4-difluoro-pheny l } -amide compositions and uses thereof.
TW201041888A (en) * 2009-05-06 2010-12-01 Plexxikon Inc Compounds and methods for kinase modulation, and indications therefor
JP5775070B2 (en) 2009-05-22 2015-09-09 インサイト・コーポレイションＩｎｃｙｔｅ Ｃｏｒｐｏｒａｔｉｏｎ N- (hetero) aryl-pyrrolidine derivatives of pyrazol-4-yl-pyrrolo [2,3-d] pyrimidine and pyrrol-3-yl-pyrrolo [2,3-d] pyrimidine as Janus kinase inhibitors
CN106967070A (en) 2009-05-22 2017-07-21 因塞特控股公司 It is used as the compound of JAK inhibitor
US20220315555A1 (en) 2009-05-26 2022-10-06 Abbvie Inc. Apoptosis inducing agents for the treatment of cancer and immune and autoimmune diseases
SG10201704742YA (en) 2009-05-26 2017-07-28 Abbvie Ireland Unlimited Co Apoptosis-inducing agents for the treatment of cancer and immune and autoimmune diseases
US8546399B2 (en) * 2009-05-26 2013-10-01 Abbvie Inc. Apoptosis inducing agents for the treatment of cancer and immune and autoimmune diseases
US9034875B2 (en) 2009-05-26 2015-05-19 Abbvie Inc. Apoptosis-inducing agents for the treatment of cancer and immune and autoimmune diseases
BRPI1011993A2 (en) 2009-06-17 2021-07-06 Vertex Pharmaceuticals Incorporated replication inhibitors of the influenza virus, their use, method of preparation and pharmaceutical composition that comprises them
AR077468A1 (en) 2009-07-09 2011-08-31 Array Biopharma Inc PIRAZOLO COMPOUNDS (1,5-A) PYRIMIDINE SUBSTITUTED AS TRK-QUINASA INHIBITORS
US8329724B2 (en) * 2009-08-03 2012-12-11 Hoffmann-La Roche Inc. Process for the manufacture of pharmaceutically active compounds
SG178092A1 (en) 2009-08-24 2012-03-29 Ascepion Pharmaceuticals Inc 5,6-bicyclic heteroaryl-containing urea compounds as kinase inhibitors
US20130018033A1 (en) 2009-08-28 2013-01-17 Array Biopharma Inc. Raf inhibitor compounds and methods of use thereof
EP2470511A1 (en) 2009-08-28 2012-07-04 Genentech, Inc. Raf inhibitor compounds and methods of use thereof
US20120157453A1 (en) 2009-08-28 2012-06-21 Genentech, Inc. Raf inhibitor compounds and methods of use thereof
JP2013503188A (en) * 2009-08-28 2013-01-31 アレイ バイオファーマ、インコーポレイテッド Raf inhibitory compounds and methods of use thereof
JP2013503190A (en) 2009-08-28 2013-01-31 アレイ バイオファーマ、インコーポレイテッド Raf inhibitory compounds and methods of use thereof
CA2772316A1 (en) 2009-08-28 2011-03-03 Array Biopharma Inc. 1h-pyrazolo [3,4-b] pyridine compounds for inhibiting raf kinase
US9249145B2 (en) 2009-09-01 2016-02-02 Incyte Holdings Corporation Heterocyclic derivatives of pyrazol-4-yl-pyrrolo[2,3-d]pyrimidines as janus kinase inhibitors
US20110274723A1 (en) 2009-10-23 2011-11-10 Mannkind Corporation Cancer immunotherapy and method of treatment
EP2496086B1 (en) 2009-11-06 2017-05-31 Plexxikon Inc. Compounds and methods for kinase modulation, and indications therefor
WO2011060216A1 (en) 2009-11-12 2011-05-19 Concert Pharmaceuticals Inc. Substituted azaindoles
NZ599872A (en) * 2009-11-18 2014-08-29 Plexxikon Inc Compounds and methods for kinase modulation, and indications therefor
DE102009058280A1 (en) * 2009-12-14 2011-06-16 Merck Patent Gmbh thiazole
MX2012007429A (en) * 2009-12-23 2012-07-23 Plexxikon Inc Compounds and methods for kinase modulation, and indications therefor.
CA2784807C (en) * 2009-12-29 2021-12-14 Dana-Farber Cancer Institute, Inc. Type ii raf kinase inhibitors
US8658170B2 (en) 2010-01-06 2014-02-25 Joseph P. Errico Combination therapy with MDM2 and EFGR inhibitors
CA2786277A1 (en) * 2010-01-06 2011-07-14 Joseph P. Errico Methods and compositions of targeted drug development
EP2533639A4 (en) 2010-02-11 2013-08-21 Univ Vanderbilt Pyrazolopyridine, pyrazolopyrazine, pyrazolopyrimidine, pyrazolothiophene and pyrazolothiazole compounds as mglur4 allosteric potentiators, compounds, and methods of treating neurological dysfunction
US11084811B2 (en) 2010-03-01 2021-08-10 Oncternal Therapeutics, Inc. Compounds for treatment of cancer
PT3354652T (en) 2010-03-10 2020-07-20 Incyte Holdings Corp Piperidin-4-yl azetidine derivatives as jak1 inhibitors
EP2550361B1 (en) 2010-03-25 2017-02-08 The J. David Gladstone Institutes Compositions and methods for treating neurological disorders
EP2558446B1 (en) 2010-04-16 2019-06-12 AC Immune S.A. Novel compounds for the treatment of diseases associated with amyloid or amyloid-like proteins
TWI619713B (en) 2010-04-21 2018-04-01 普雷辛肯公司 Compounds and methods for kinase modulation, and indications therefor
AU2011248175A1 (en) * 2010-05-06 2012-10-25 Merck Sharp & Dohme Corp. Aza-Indole derivatives useful as modulators of FAAH
AR081039A1 (en) 2010-05-14 2012-05-30 Osi Pharmaceuticals Llc QUINASA FUSIONED BICYCLE INHIBITORS
EP2569315A1 (en) 2010-05-14 2013-03-20 OSI Pharmaceuticals, LLC Fused bicyclic kinase inhibitors
AU2011256380C1 (en) 2010-05-20 2016-09-08 Array Biopharma Inc. Macrocyclic compounds as Trk kinase inhibitors
KR102303885B1 (en) 2010-05-21 2021-09-24 인사이트 홀딩스 코포레이션 Topical formulation for a jak inhibitor
US8669256B2 (en) 2010-05-28 2014-03-11 Merck Sharp & Dohme B.V. Substituted thieno[2,3-b]pyrazine compounds as modulators of B-Raf kinase activity
NZ702485A (en) 2010-06-03 2016-04-29 Pharmacyclics Llc The use of inhibitors of bruton’s tyrosine kinase (btk)
EP2580336B1 (en) 2010-06-09 2017-05-10 Bayer CropScience NV Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering
US8779150B2 (en) 2010-07-21 2014-07-15 Hoffmann-La Roche Inc. Processes for the manufacture of propane-1-sulfonic acid {3-[5-(4-chloro-phenyl)-1 H-pyrrolo[2,3-b]pyridine-3-carbonyl]-2,4-difluoro-phenyl}-amide
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
JP5933544B2 (en) 2010-07-26 2016-06-08 バイオマトリカ， インコーポレーテッド Compositions for stabilizing blood DNA, RNA and proteins and other biological samples shipped and stored at ambient temperature
US8709419B2 (en) * 2010-08-17 2014-04-29 Hoffmann-La Roche, Inc. Combination therapy
US20120045433A1 (en) 2010-08-17 2012-02-23 Kapil Dhingra Combination therapy
EP2616467A1 (en) 2010-09-13 2013-07-24 Concert Pharmaceuticals Inc. Substituted azaindoles
UA113500C2 (en) 2010-10-29 2017-02-10 MEL EXTRUSION SOLID DISPERSIONS CONTAINING AN APOPTOSIS-INDUCING AGENT
US20120277210A1 (en) 2010-10-29 2012-11-01 Abbott Laboratories Solid dispersions containing an apoptosis-inducing agent
KR101236731B1 (en) 2010-11-02 2013-02-25 한국과학기술원 Azaindole compounds, PI3K inhibitoring composition and composition used in diseases linked to PI3K comprising the same
WO2012068450A1 (en) 2010-11-19 2012-05-24 Incyte Corporation Cyclobutyl substituted pyrrolopyridine and pyrrolopyrimidine derivatives as jak inhibitors
ES2536415T3 (en) 2010-11-19 2015-05-25 Incyte Corporation Pyrrolopyridines and heterocyclic substituted pyrrolopyrimidines as JAK inhibitors
NZ610746A (en) 2010-11-23 2015-06-26 Abbvie Bahamas Ltd Methods of treatment using selective bcl-2 inhibitors
JP6141188B2 (en) 2010-11-23 2017-06-07 アッヴィ・インコーポレイテッド Salt and crystal forms of apoptosis inducers
US8771687B2 (en) 2010-12-02 2014-07-08 University of Pittsburgh—of the Commonwealth System of Higher Education Methods for treating a tumor using an antibody that specifically binds GRP94
US9295669B2 (en) * 2010-12-14 2016-03-29 Hoffman La-Roche Inc. Combination therapy for proliferative disorders
CA2822057A1 (en) 2010-12-16 2012-06-21 Vertex Pharmaceuticals Incorporated Inhibitors of influenza viruses replication
US8937088B2 (en) 2011-01-06 2015-01-20 Astar Biotech Llc Ureas for the treatment and prevention of cancer
CA2826123C (en) 2011-02-07 2016-08-09 Plexxikon Inc. Compounds and methods for kinase modulation, and indications therefor
US20140031383A1 (en) 2011-02-08 2014-01-30 Dana-Farber Cancer Institute, Inc. Methods for treatment of melanoma
ES2547916T3 (en) 2011-02-18 2015-10-09 Novartis Pharma Ag MTOR / JAK inhibitor combination therapy
TWI558702B (en) 2011-02-21 2016-11-21 普雷辛肯公司 Solid forms of a pharmaceutically active substance
US20140199292A1 (en) * 2011-03-15 2014-07-17 Inserm (Institut National De La Sante Et De La Recherche Medicale) Compositions and methods for inhibiting tumor development caused by chemotherapy induced senescence
US9150549B2 (en) * 2011-04-01 2015-10-06 Genentech, Inc. Combinations of AKT inhibitor compounds and erlotinib, and methods of use
WO2012138809A1 (en) * 2011-04-05 2012-10-11 Dawei Zhang Heterocyclic compounds as kinase inhibitors
RU2580609C2 (en) * 2011-04-18 2016-04-10 Эйсай Ар Энд Ди Менеджмент Ко., Лтд. Anticancer therapeutic agent
CA2872857A1 (en) * 2011-05-10 2012-11-15 Brunangelo Falini Hairy cell leukemia biomarkers and methods of using same
US20140088114A1 (en) 2011-05-16 2014-03-27 OSI Pharmaceuticals ,LLC Fused bicyclic kinase inhibitors
BR112013029163A2 (en) 2011-05-17 2017-01-31 Plexxikon Inc kinase modulation and indications thereof
EP2714688B1 (en) 2011-05-26 2016-02-24 Daiichi Sankyo Company, Limited Heterocyclic compounds as protein kinase inhibitors
ES2705950T3 (en) 2011-06-03 2019-03-27 Eisai R&D Man Co Ltd Biomarkers to predict and assess the responsiveness of subjects with thyroid and kidney cancer to lenvatinib compounds
JP2014516084A (en) 2011-06-10 2014-07-07 グラクソスミスクライン、インテレクチュアル、プロパティー、リミテッド New compounds
BR112013032720A2 (en) 2011-06-20 2016-09-13 Incyte Corp "azetidinyl phenyl, pyridyl or pyrazinyl carboxamide derivatives as jak inhibitors, composition and use of said derivatives"
EA201490265A1 (en) 2011-07-13 2014-12-30 Фармасайкликс, Инк. BLUTON TYROSINKINASE INHIBITORS
KR20190133790A (en) 2011-08-01 2019-12-03 제넨테크, 인크. Methods of treating cancer using pd-1 axis binding antagonists and mek inhibitors
UA118010C2 (en) 2011-08-01 2018-11-12 Вертекс Фармасьютікалз Інкорпорейтед INFLUENCES OF INFLUENZA VIRUS REPLICATION
EP2741747A1 (en) 2011-08-10 2014-06-18 Novartis Pharma AG JAK P13K/mTOR COMBINATION THERAPY
TW201313721A (en) 2011-08-18 2013-04-01 Incyte Corp Cyclohexyl azetidine derivatives as JAK inhibitors
WO2013029043A1 (en) * 2011-08-25 2013-02-28 Reaction Biology Corp. Selective kinase inhibitors
CN107245056A (en) 2011-08-26 2017-10-13 润新生物公司 Chemical entities, composition and method
UA111854C2 (en) 2011-09-07 2016-06-24 Інсайт Холдінгс Корпорейшн METHODS AND INTERMEDIATE COMPOUNDS FOR JAK INHIBITORS
CN115403531A (en) 2011-09-14 2022-11-29 润新生物公司 Chemical entities, compositions, and methods as kinase inhibitors
EP2570127A1 (en) 2011-09-16 2013-03-20 Sanofi Compositions and methods for treating cancer using PI3KB beta inhibitor and MAPK pathway inhibitor, including MEK and RAF inhibitors
WO2013043715A1 (en) 2011-09-19 2013-03-28 Genentech, Inc. Combination treatments comprising c-met antagonists and b-raf antagonists
EP2771011A4 (en) * 2011-10-24 2015-04-15 Glaxosmithkline Ip No 2 Ltd Chemical compounds
JP6106685B2 (en) 2011-11-17 2017-04-05 ダナ−ファーバー キャンサー インスティテュート， インコーポレイテッド Inhibitors of C-JUN-N-terminal kinase (JNK)
US8377946B1 (en) 2011-12-30 2013-02-19 Pharmacyclics, Inc. Pyrazolo[3,4-d]pyrimidine and pyrrolo[2,3-d]pyrimidine compounds as kinase inhibitors
IN2014DN06166A (en) 2011-12-31 2015-08-21 Beigene Ltd
WO2013109142A1 (en) 2012-01-16 2013-07-25 Stichting Het Nederlands Kanker Instituut Combined pdk and mapk/erk pathway inhibition in neoplasia
CN102603734A (en) * 2012-01-19 2012-07-25 盛世泰科生物医药技术(苏州)有限公司 Protein kinase inhibitor and application thereof
US9670180B2 (en) 2012-01-25 2017-06-06 Neupharma, Inc. Certain chemical entities, compositions, and methods
US9358235B2 (en) 2012-03-19 2016-06-07 Plexxikon Inc. Kinase modulation, and indications therefor
US9216170B2 (en) 2012-03-19 2015-12-22 Hoffmann-La Roche Inc. Combination therapy for proliferative disorders
ES2768602T3 (en) 2012-03-28 2020-06-23 UCB Biopharma SRL Phenyl-urea and phenyl-carbamate derivatives as inhibitors of protein aggregation
BR112014026367A2 (en) * 2012-04-23 2017-06-27 Genentech Inc 5-bromo-4-chloro-3-nitro-1h-pyrrol [2,3-b] pyridine and its process
WO2013173720A1 (en) 2012-05-18 2013-11-21 Incyte Corporation Piperidinylcyclobutyl substituted pyrrolopyridine and pyrrolopyrimidine derivatives as jak inhibitors
EP2858501A4 (en) * 2012-05-22 2015-12-09 Merck Sharp & Dohme TrkA KINASE INHIBITORS, COMPOSITIONS AND METHODS THEREOF
US10077474B2 (en) * 2012-05-29 2018-09-18 Abbott Molecular, Inc. Method of designing primers, method of detecting single nucleotide polymorphisms (SNPs), method of distinguishing SNPs, and related primers, detectable oligonucleotides, and kits
CA2870407A1 (en) 2012-05-31 2013-12-05 F. Hoffmann-La Roche Ag Aminoquinazoline and pyridopyrimidine derivatives
US9150570B2 (en) * 2012-05-31 2015-10-06 Plexxikon Inc. Synthesis of heterocyclic compounds
CA2991994C (en) 2012-06-04 2022-06-28 Pharmacyclics Llc Pharmaceutical formulation for oral administration comprising ibrutinib
JP2015522037A (en) 2012-07-03 2015-08-03 ラシオファルム ゲーエムベーハー Solid form of Vemurafenib choline salt
CA2879570A1 (en) 2012-07-24 2014-01-30 Pharmacyclics, Inc. Mutations associated with resistance to inhibitors of bruton's tyrosine kinase (btk)
US20150183781A1 (en) * 2012-08-07 2015-07-02 Um Pharmauji Sdn. Bhd. 5-(1H-Pyrazol-4-yl)-1H-Pyrrolo[2,3-b]Pyridine Derivatives as Kinase Inhibitors
BR112015003418A2 (en) 2012-08-17 2017-07-04 Hoffmann La Roche pharmaceutical, combined, methods for extending the duration of treatment response, delaying or preventing the development of treatment resistance, selecting a therapy, and optimizing therapeutic efficacy.
TWI601725B (en) * 2012-08-27 2017-10-11 加拓科學公司 Substituted azaindole compounds, salts, pharmaceutical compositions thereof and methods of use
CN103626765B (en) * 2012-08-27 2016-08-10 广东东阳光药业有限公司 Substituted azaindole compounds and salt, compositions and purposes
EP2892534B8 (en) 2012-09-06 2021-09-15 Plexxikon Inc. Compounds and methods for kinase modulation, and indications therefor
US9688635B2 (en) 2012-09-24 2017-06-27 Neupharma, Inc. Certain chemical entities, compositions, and methods
CN103613591B (en) * 2012-09-29 2016-04-13 上海科州药物研发有限公司 As the compound and its production and use of cMet inhibitor
CA2885259A1 (en) 2012-10-04 2014-04-10 Pfizer Limited Pyrrolo[3,2-c]pyridine tropomyosin-related kinase inhibitors
CN109276716B (en) * 2012-10-08 2021-06-08 归属疗法有限公司 MEK inhibitors for use in the treatment of viral diseases
WO2014063068A1 (en) 2012-10-18 2014-04-24 Dana-Farber Cancer Institute, Inc. Inhibitors of cyclin-dependent kinase 7 (cdk7)
US10000483B2 (en) 2012-10-19 2018-06-19 Dana-Farber Cancer Institute, Inc. Bone marrow on X chromosome kinase (BMX) inhibitors and uses thereof
US9758522B2 (en) 2012-10-19 2017-09-12 Dana-Farber Cancer Institute, Inc. Hydrophobically tagged small molecules as inducers of protein degradation
WO2014075077A1 (en) 2012-11-12 2014-05-15 Neupharma, Inc. Certain chemical entities, compositions, and methods
MY191357A (en) 2012-11-15 2022-06-19 Incyte Holdings Corp Sustained-release dosage forms of ruxolitinib
CA2890934A1 (en) 2012-11-15 2014-05-22 Pharmacyclics, Inc. Pyrrolopyrimidine compounds as kinase inhibitors
US9260426B2 (en) 2012-12-14 2016-02-16 Arrien Pharmaceuticals Llc Substituted 1H-pyrrolo [2, 3-b] pyridine and 1H-pyrazolo [3, 4-b] pyridine derivatives as salt inducible kinase 2 (SIK2) inhibitors
WO2014100755A2 (en) 2012-12-20 2014-06-26 Biomatrica, Inc. Formulations and methods for stabilizing pcr reagents
ES2664985T3 (en) 2012-12-21 2018-04-24 Plexxikon Inc. Compounds and methods for kinase modulation, and indications thereof
MX2015004979A (en) 2012-12-21 2015-07-17 Eisai R&D Man Co Ltd Amorphous form of quinoline derivative, and method for producing same.
WO2014110574A1 (en) 2013-01-14 2014-07-17 Incyte Corporation Bicyclic aromatic carboxamide compounds useful as pim kinase inhibitors
CN105051029B (en) 2013-01-15 2018-07-31 因赛特公司 It can be used as the thiazole carboxamides and pyridinecarboxylic amine compounds of PIM kinase inhibitors
US20140357636A1 (en) * 2013-02-21 2014-12-04 Wayne Rothbaum Treatment of Skeletal-Related Disorders
JP2016509045A (en) 2013-02-22 2016-03-24 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト How to treat cancer and prevent drug resistance
US10022356B2 (en) * 2013-03-05 2018-07-17 University Of Tennessee Research Foundation Compounds for treatment of cancer
ES2900492T3 (en) 2013-03-06 2022-03-17 Incyte Holdings Corp Processes and intermediates to make a JAK inhibitor
US20140275082A1 (en) 2013-03-14 2014-09-18 Abbvie Inc. Apoptosis-inducing agents for the treatment of cancer and immune and autoimmune diseases
EP2970270A1 (en) 2013-03-14 2016-01-20 ratiopharm GmbH Solid state forms of vemurafenib hydrochloride
US20140303121A1 (en) 2013-03-15 2014-10-09 Plexxikon Inc. Heterocyclic compounds and uses thereof
US20160143910A1 (en) 2013-03-15 2016-05-26 Constellation Pharmaceuticals, Inc. Methods of treating cancer and preventing cancer drug resistance
JP2016516682A (en) 2013-03-15 2016-06-09 サノフイ Anti-tumor composition comprising PI3Kbeta inhibitor and RAF inhibitor for overcoming cancer cell resistance
TWI634111B (en) 2013-03-15 2018-09-01 普雷辛肯公司 Heterocyclic compounds and uses thereof
US9206188B2 (en) 2013-04-18 2015-12-08 Arrien Pharmaceuticals Llc Substituted pyrrolo[2,3-b]pyridines as ITK and JAK inhibitors
WO2014185540A1 (en) 2013-05-14 2014-11-20 Eisai R&D Management Co., Ltd. Biomarkers for predicting and assessing responsiveness of endometrial cancer subjects to lenvatinib compounds
EP3004060B1 (en) 2013-05-30 2019-11-27 Plexxikon Inc. Compounds for kinase modulation, and indications therefor
WO2015002729A2 (en) 2013-06-13 2015-01-08 Biomatrica, Inc. Cell stabilization
WO2014206344A1 (en) 2013-06-28 2014-12-31 Beigene, Ltd. Fused tricyclic amide compounds as multiple kinase inhibitors
CN105530931B (en) * 2013-07-12 2019-11-08 皮拉马尔企业有限公司 For treating the pharmaceutical composition of melanoma
EP3027192A4 (en) 2013-08-02 2017-03-22 Pharmacyclics, LLC Methods for the treatment of solid tumors
KR102419714B1 (en) 2013-08-07 2022-07-13 인사이트 코포레이션 Sustained release dosage forms for a jak1 inhibitor
ES2709509T3 (en) 2013-08-12 2019-04-16 Pharmacyclics Llc Procedures for the treatment of cancer amplified by HER2
JP2016528298A (en) 2013-08-23 2016-09-15 インサイト・コーポレイションＩｎｃｙｔｅ Ｃｏｒｐｏｒａｔｉｏｎ Furopyridine and thienopyridinecarboxamide compounds useful as PIM kinase inhibitors
WO2015041534A1 (en) 2013-09-20 2015-03-26 Stichting Het Nederlands Kanker Instituut P90rsk in combination with raf/erk/mek
US9629851B2 (en) 2013-09-20 2017-04-25 Stitching Het Nederlands Kanker Institut—Antoni Van Leeuwenhoek Ziekenhuis ROCK in combination with MAPK pathway
CN105764896A (en) 2013-09-30 2016-07-13 药品循环有限责任公司 Inhibitors of Bruton's tyrosine kinase
AU2014337122B2 (en) 2013-10-18 2019-01-03 Dana-Farber Cancer Institute, Inc. Heteroaromatic compounds useful for the treatment of proliferative diseases
AU2014337044A1 (en) 2013-10-18 2016-05-05 Dana-Farber Cancer Institute, Inc. Polycyclic inhibitors of cyclin-dependent kinase 7 (CDK7)
PT3068782T (en) 2013-11-13 2018-10-08 Vertex Pharma Methods of preparing inhibitors of influenza viruses replication
PT3068776T (en) 2013-11-13 2019-08-26 Vertex Pharma Inhibitors of influenza viruses replication
WO2015073864A1 (en) * 2013-11-15 2015-05-21 The Wisar Institute Of Anatomy And Biology Ebna1 inhibitors and their method of use
WO2015075749A1 (en) * 2013-11-22 2015-05-28 Laurus Labs Private Limited Novel processes for the preparation of vemurafenib
CZ2013943A3 (en) 2013-11-27 2015-06-03 Zentiva, K.S. Vemurafenib crystalline forms
CN104710417B (en) * 2013-12-11 2020-09-08 上海科州药物研发有限公司 Azaindole derivatives and synthesis method thereof
ES2909910T3 (en) * 2013-12-20 2022-05-10 Biomed Valley Discoveries Inc Cancer treatment using combinations of ERK and RAF inhibitors
CN104739823A (en) * 2013-12-28 2015-07-01 复旦大学附属华山医院 Application of icaritin in preparation of vemurafenib sensitizer
WO2015134536A1 (en) 2014-03-04 2015-09-11 Plexxikon Inc. Compounds and methods for kinase modulation, and indications therefor
JP2017509336A (en) 2014-03-20 2017-04-06 ファーマサイクリックス エルエルシー Mutations associated with phospholipase C gamma 2 and resistance
US20170027940A1 (en) 2014-04-10 2017-02-02 Stichting Het Nederlands Kanker Instituut Method for treating cancer
WO2015164604A1 (en) 2014-04-23 2015-10-29 Dana-Farber Cancer Institute, Inc. Hydrophobically tagged janus kinase inhibitors and uses thereof
WO2015164614A1 (en) 2014-04-23 2015-10-29 Dana-Farber Cancer Institute, Inc. Janus kinase inhibitors and uses thereof
WO2015171833A1 (en) * 2014-05-06 2015-11-12 The Regents Of The University Of California Wound healing using braf inhibitors
WO2015178770A1 (en) 2014-05-19 2015-11-26 Stichting Het Nederlands Kanker Instituut Compositions for cancer treatment
WO2015181628A1 (en) * 2014-05-27 2015-12-03 Eisai R&D Management Co., Ltd. Treatment of acute myeloid leukemia with an hck inhibitor
WO2015184305A1 (en) 2014-05-30 2015-12-03 Incyte Corporation TREATMENT OF CHRONIC NEUTROPHILIC LEUKEMIA (CNL) AND ATYPICAL CHRONIC MYELOID LEUKEMIA (aCML) BY INHIBITORS OF JAK1
TW201613916A (en) * 2014-06-03 2016-04-16 Gilead Sciences Inc TANK-binding kinase inhibitor compounds
ES2786373T3 (en) 2014-06-10 2020-10-09 Biomatrica Inc Platelet stabilization at room temperatures
CN107073121A (en) 2014-06-13 2017-08-18 基因泰克公司 Treatment and the method for prevention cancer drug resistance
WO2015191996A1 (en) * 2014-06-13 2015-12-17 Genentech, Inc. Methods of treating and preventing cancer drug resistance
TW201613919A (en) 2014-07-02 2016-04-16 Pharmacyclics Llc Inhibitors of Bruton's tyrosine kinase
WO2016000615A1 (en) * 2014-07-02 2016-01-07 Sunshine Lake Pharma Co., Ltd. Heteroaryl compounds and pharmaceutical applications thereof
US9822124B2 (en) 2014-07-14 2017-11-21 Incyte Corporation Bicyclic heteroaromatic carboxamide compounds useful as Pim kinase inhibitors
US9580418B2 (en) 2014-07-14 2017-02-28 Incyte Corporation Bicyclic aromatic carboxamide compounds useful as Pim kinase inhibitors
PL3169361T3 (en) 2014-07-15 2019-11-29 Hoffmann La Roche Compositions for treating cancer using pd-1 axis binding antagonists and mek inhibitors
US9533991B2 (en) 2014-08-01 2017-01-03 Pharmacyclics Llc Inhibitors of Bruton's tyrosine kinase
JP2017523206A (en) 2014-08-07 2017-08-17 ファーマサイクリックス エルエルシー New formulation of breton-type tyrosine kinase inhibitor
PL3524595T3 (en) 2014-08-28 2022-10-31 Eisai R&D Management Co., Ltd. High-purity quinoline derivative and method for manufacturing same
EP3194392B1 (en) 2014-09-15 2020-01-01 Plexxikon, Inc. Heterocyclic compounds and uses thereof
CN106715419A (en) 2014-09-26 2017-05-24 吉利德科学公司 Aminotriazine derivatives useful as TANK-binding kinase inhibitor compounds
US20170248603A1 (en) 2014-10-06 2017-08-31 Dana-Farber Cancer Institute, Inc. Angiopoiten-2 biomarkers predictive of anti-immune checkpoint response
WO2016073771A2 (en) * 2014-11-06 2016-05-12 Ohio State Innovation Foundation Pyrrolopyrimidine derivatives as mps1/ttk kinase inhibitors
WO2016083956A1 (en) * 2014-11-29 2016-06-02 Shilpa Medicare Limited Substantially pure vemurafenib and its salts
US9694084B2 (en) 2014-12-23 2017-07-04 Dana-Farber Cancer Institute, Inc. Methods to induce targeted protein degradation through bifunctional molecules
EP3256470B1 (en) 2014-12-23 2023-07-26 Dana-Farber Cancer Institute, Inc. Methods to induce targeted protein degradation through bifunctional molecules
EP3236959A4 (en) 2014-12-23 2018-04-25 Dana Farber Cancer Institute, Inc. Inhibitors of cyclin-dependent kinase 7 (cdk7)
MX2017010474A (en) 2015-02-25 2017-11-28 Eisai R&D Man Co Ltd Method for suppressing bitterness of quinoline derivative.
PT3265084T (en) 2015-03-03 2024-02-13 Pharmacyclics Llc Pharmaceutical formulations of bruton's tyrosine kinase inhibtor
AU2015384801B2 (en) 2015-03-04 2022-01-06 Eisai R&D Management Co., Ltd. Combination of a PD-1 antagonist and a VEGFR/FGFR/RET tyrosine kinase inhibitor for treating cancer
EP3273966B1 (en) 2015-03-27 2023-05-03 Dana-Farber Cancer Institute, Inc. Inhibitors of cyclin-dependent kinases
WO2016164641A1 (en) 2015-04-08 2016-10-13 Plexxikon Inc. Compounds and methods for kinase modulation, and indications therefor
CZ2015250A3 (en) 2015-04-14 2016-10-26 Zentiva, K.S. Vemurafenib amorphous forms
US9745298B2 (en) 2015-05-06 2017-08-29 Plexxikon Inc. Synthesis of a compound that modulates kinases
HUE050506T2 (en) 2015-05-06 2020-12-28 Plexxikon Inc Solid forms of a compound modulating kinases
WO2016183116A1 (en) 2015-05-13 2016-11-17 Vertex Pharmaceuticals Incorporated Methods of preparing inhibitors of influenza viruses replication
MA42422A (en) 2015-05-13 2018-05-23 Vertex Pharma INHIBITORS OF INFLUENZA VIRUS REPLICATION
CN107848964B (en) 2015-05-14 2021-03-19 威斯塔解剖学和生物学研究所 EBNA1 inhibitors and methods of using the same
BR112017025045A2 (en) 2015-05-22 2018-08-07 Plexxikon Inc plx-8394 or plx-7904 for use in treating braf-v600 related diseases
US9776998B2 (en) 2015-05-22 2017-10-03 Plexxikon Inc. Synthesis of heterocyclic compounds
US9540347B2 (en) 2015-05-29 2017-01-10 Incyte Corporation Pyridineamine compounds useful as Pim kinase inhibitors
PE20230683A1 (en) * 2015-06-09 2023-04-21 Abbvie Inc NUCLEAR RECEIVER MODULATORS
AU2016276963C1 (en) 2015-06-12 2021-08-05 Dana-Farber Cancer Institute, Inc. Combination therapy of transcription inhibitors and kinase inhibitors
MX2017015896A (en) 2015-06-16 2018-08-09 Eisai R&D Man Co Ltd Anticancer agent.
BR112018000808A2 (en) 2015-07-16 2018-09-04 Array Biopharma Inc substituted pyrazolo [1,5-a] pyridine compounds as retinase inhibitors
AR105392A1 (en) * 2015-07-20 2017-09-27 Genzyme Corp COLONIA STIMULATOR FACTOR RECEIVER INHIBITORS 1 (CSF-1R)
WO2017019804A2 (en) 2015-07-28 2017-02-02 Plexxikon Inc. Compounds and methods for kinase modulation, and indications therefor
US10729669B2 (en) * 2015-07-28 2020-08-04 University Of Iowa Research Foundation Compositions and methods for treating cancer
AR105967A1 (en) 2015-09-09 2017-11-29 Incyte Corp SALTS OF A PIM QUINASA INHIBITOR
EP3347018B1 (en) 2015-09-09 2021-09-01 Dana-Farber Cancer Institute, Inc. Inhibitors of cyclin-dependent kinases
TWI732785B (en) 2015-09-21 2021-07-11 美商普雷辛肯公司 Heterocyclic compounds and uses thereof
TW201718546A (en) 2015-10-02 2017-06-01 英塞特公司 Heterocyclic compounds useful as PIM kinase inhibitors
US9890163B2 (en) 2015-10-15 2018-02-13 Princeton Drug Discovery Inc Inhibitors of protein kinases
SG11201803438XA (en) 2015-10-26 2018-05-30 Univ Colorado Regents Point mutations in trk inhibitor-resistant cancer and methods relating to the same
ES2824120T3 (en) 2015-11-19 2021-05-11 Hoffmann La Roche Cancer treatment procedures using b-raf inhibitors and immune checkpoint inhibitors
AU2016367147B2 (en) 2015-12-07 2021-04-08 Plexxikon Inc. Compounds and methods for kinase modulation, and indications therefor
EP3387411B1 (en) 2015-12-08 2023-05-10 Biomatrica, INC. Reduction of erythrocyte sedimentation rate
KR20180093056A (en) 2015-12-14 2018-08-20 레드.컴, 엘엘씨 Modular digital cameras and cellular phones
BR112017002594A2 (en) 2015-12-17 2017-12-19 Gilead Sciences Inc tank binding kinase inhibitor compounds
US10513528B2 (en) * 2016-02-25 2019-12-24 Taxis Pharmaceuticals, Inc. Synthetic processes and intermediates
AU2017232610B2 (en) 2016-03-16 2021-07-22 Plexxikon Inc. Compounds and methods for kinase modulation and indications therefore
CA3019661A1 (en) 2016-04-04 2017-10-12 Loxo Oncology, Inc. Methods of treating pediatric cancers
US10045991B2 (en) 2016-04-04 2018-08-14 Loxo Oncology, Inc. Methods of treating pediatric cancers
SG11201808559PA (en) 2016-04-04 2018-10-30 Loxo Oncology Inc Liquid formulations of (s)-n-(5-((r)-2-(2,5-difluorophenyl)-pyrrolidin-1-yl)-pyrazolo[1,5-a]pyrimidin-3-yl)-3-hydroxypyrrolidine-1-carboxamide
EP3443114A1 (en) 2016-04-15 2019-02-20 H. Hoffnabb-La Roche Ag Diagnostic and therapeutic methods for cancer
KR102566858B1 (en) 2016-05-18 2023-08-11 어레이 바이오파마 인크. (S)-N-(5-((R)-2-(2,5-difluorophenyl)pyrrolidin-1-yl)-pyrazolo[1,5-A]pyrimidin-3-yl Method for producing )-3-hydroxypyrrolidine-1-carboxamide
AU2017283491B2 (en) * 2016-06-13 2021-06-03 Chemocentryx, Inc. Methods of treating pancreatic cancer
US11179484B2 (en) 2016-06-24 2021-11-23 University Of Iowa Research Foundation Compositions and methods of treating melanoma
US10407427B2 (en) 2016-07-01 2019-09-10 Fermion Oy Processes for the preparation of Vemurafenib
SG11201900157RA (en) 2016-07-12 2019-02-27 Revolution Medicines Inc 2,5-disubstituted 3-methyl pyrazines and 2,5,6-trisubstituted 3-methyl pyrazines as allosteric shp2 inhibitors
CN109715631B (en) * 2016-09-16 2022-02-18 德国亥姆霍兹慕尼黑中心健康与环境研究中心（有限公司） TRAF6 inhibitors
TW201815766A (en) 2016-09-22 2018-05-01 美商普雷辛肯公司 Compounds and methods for IDO and TDO modulation, and indications therefor
TWI704148B (en) 2016-10-10 2020-09-11 美商亞雷生物製藥股份有限公司 Substituted pyrazolo[1,5-a]pyridine compounds as ret kinase inhibitors
JOP20190077A1 (en) 2016-10-10 2019-04-09 Array Biopharma Inc Substituted pyrazolo[1,5-a]pyridine compounds as ret kinase inhibitors
EP3308773A1 (en) 2016-10-11 2018-04-18 Recordati Industria Chimica E Farmaceutica SPA Formulations of cysteamine and cysteamine derivatives
CA3040348A1 (en) 2016-10-19 2018-04-26 United States Government As Represented By The Department Of Veterans Affairs Compositions and methods for treating cancer
JOP20190092A1 (en) 2016-10-26 2019-04-25 Array Biopharma Inc PROCESS FOR THE PREPARATION OF PYRAZOLO[1,5-a]PYRIMIDINES AND SALTS THEREOF
EP4134080A1 (en) 2016-11-23 2023-02-15 ChemoCentryx, Inc. Ccr2 inhibitors for use in treating renal diseases
CA3025173A1 (en) 2016-12-15 2018-06-21 Nestec S.A. Compositions and methods that modulate phosphorus or enzymes in a companion animal
US11173211B2 (en) 2016-12-23 2021-11-16 Arvinas Operations, Inc. Compounds and methods for the targeted degradation of rapidly accelerated Fibrosarcoma polypeptides
US10703757B2 (en) 2016-12-23 2020-07-07 Plexxikon Inc. Compounds and methods for CDK8 modulation and indications therefor
EP3558994A4 (en) * 2016-12-23 2021-05-12 Arvinas Operations, Inc. Compounds and methods for the targeted degradation of rapidly accelerated fibrosarcoma polypeptides
MX2019008458A (en) * 2017-01-17 2019-12-02 Heparegenix Gmbh Protein kinase inhibitors for promoting liver regeneration or reducing or preventing hepatocyte death.
WO2018136663A1 (en) 2017-01-18 2018-07-26 Array Biopharma, Inc. Ret inhibitors
EP3571203B1 (en) 2017-01-18 2023-06-07 Array BioPharma Inc. Substituted pyrazolo[1,5-a]pyrazine compounds as ret kinase inhibitors
TWI820013B (en) 2017-01-23 2023-11-01 美商銳新醫藥公司 Bicyclic compounds as allosteric shp2 inhibitors
JP7240320B2 (en) 2017-01-23 2023-03-15 レヴォリューション・メディスンズ，インコーポレイテッド Pyridine compounds as allosteric SHP2 inhibitors
EP3579872A1 (en) 2017-02-10 2019-12-18 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and pharmaceutical compositions for the treatment of cancers associated with activation of the mapk pathway
JOP20190213A1 (en) 2017-03-16 2019-09-16 Array Biopharma Inc Macrocyclic compounds as ros1 kinase inhibitors
EP3601326A4 (en) * 2017-03-20 2020-12-16 The Broad Institute, Inc. Compounds and methods for regulating insulin secretion
WO2018175311A1 (en) 2017-03-20 2018-09-27 Plexxikon Inc. Crystalline forms of 4-(1-(1,1-di(pyridin-2-yl)ethyl)-6-(3,5-dimethylisoxazol-4-yl)-1h- pyrrolo[3,2-b]pyridin-3-yl)benzoic acid that inhibits bromodomain
US11285154B2 (en) 2017-03-29 2022-03-29 United States Government As Represented By The Department Of Veterans Affairs Methods and compositions for treating cancer
US10774093B2 (en) 2017-03-30 2020-09-15 Taxis Pharmaceuticals, Inc. Synthetic processes and synthetic intermediates
CN110545853A (en) * 2017-04-28 2019-12-06 石英治疗有限公司 RAF degrading coupled compounds
WO2018210661A1 (en) * 2017-05-15 2018-11-22 Basf Se Heteroaryl compounds as agrochemical fungicides
EP3624795B1 (en) 2017-05-19 2022-04-20 NFlection Therapeutics, Inc. Fused heteroaromatic-aniline compounds for treatment of dermal disorders
KR102642411B1 (en) * 2017-05-19 2024-02-28 엔플렉션 테라퓨틱스, 인코포레이티드 Pyrrolopyridine-aniline compounds for the treatment of skin diseases
WO2018226846A1 (en) 2017-06-07 2018-12-13 Plexxikon Inc. Compounds and methods for kinase modulation
CN110944634A (en) 2017-06-30 2020-03-31 加利福尼亚大学董事会 Compositions and methods for regulating hair growth
WO2019023198A1 (en) 2017-07-25 2019-01-31 Plexxikon Inc. Formulations of a compound modulating kinases
CN109384785B (en) * 2017-08-10 2021-09-28 浙江海正药业股份有限公司 Pyrrolopyridinone derivatives, preparation method and medical application thereof
EP3679159A1 (en) 2017-09-08 2020-07-15 H. Hoffnabb-La Roche Ag Diagnostic and therapeutic methods for cancer
TWI791053B (en) 2017-10-10 2023-02-01 美商亞雷生物製藥股份有限公司 Crystalline forms of 6-(2-hydroxy-2-methylpropoxy)-4-(6-(6-((6-methoxypyridin-3-yl)methyl)-3,6-diazabicyclo[3.1.1]heptan-3-yl)pyridin-3-yl)pyrazolo[1,5-a]pyridine-3-carbonitrile and pharmaceutical composition thereof
TWI812649B (en) 2017-10-10 2023-08-21 美商絡速藥業公司 Formulations of 6-(2-hydroxy-2-methylpropoxy)-4-(6-(6-((6-methoxypyridin-3-yl)methyl)-3,6-diazabicyclo[3.1.1]heptan-3-yl)pyridin-3-yl)pyrazolo[1,5-a]pyridine-3-carbonitrile
BR112020007183A2 (en) 2017-10-11 2020-09-24 Chemocentryx, Inc. treatment of segmented focal glomerulosclerosis with ccr2 antagonists
RU2020115095A (en) 2017-10-12 2021-11-12 Революшн Медсинз, Инк. PYRIDINE, PYRAZINE AND TRIAZINE COMPOUNDS AS ALLOSTERIC SHP2 INHIBITORS
AU2018348241B2 (en) 2017-10-13 2023-01-12 Opna Bio SA Solid forms of a compound for modulating kinases
US11306086B2 (en) 2017-10-26 2022-04-19 Xynomic Pharmaceuticals, Inc. Crystalline salts of a B-RAF kinase inhibitor
US11103505B2 (en) 2017-10-27 2021-08-31 Plexxikon Inc. Formulations of a compound modulating kinases
AR113922A1 (en) 2017-12-08 2020-07-01 Incyte Corp LOW DOSE COMBINATION THERAPY FOR THE TREATMENT OF MYELOPROLIFERATIVE NEOPLASMS
BR112020009757A2 (en) 2017-12-15 2020-11-03 Revolution Medicines, Inc. polycyclic compounds as allosteric inhibitors of shp2
CA3086765A1 (en) 2017-12-28 2019-07-04 Tract Pharmaceuticals, Inc. Stem cell culture systems for columnar epithelial stem cells, and uses related thereto
CA3087354C (en) 2018-01-18 2023-01-03 Array Biopharma Inc. Substituted pyrrolo[2,3-d]pyrimidines compounds as ret kinase inhibitors
CN111630054B (en) 2018-01-18 2023-05-09 奥瑞生物药品公司 Substituted pyrazolo [3,4-d ] pyrimidine compounds as RET kinase inhibitors
JP6997876B2 (en) 2018-01-18 2022-02-04 アレイ バイオファーマ インコーポレイテッド Substituted pyrazolyl [4,3-C] pyridine compound as a RET kinase inhibitor
HRP20220510T1 (en) 2018-01-30 2022-05-27 Incyte Corporation Processes for preparing (1 -(3-fluoro-2-(trifluoromethyl)isonicotinyl)piperidine-4-one)
MA51889A (en) 2018-01-31 2021-05-05 Heparegenix Gmbh KINASE MKK4 PROTEIN INHIBITORS TO PROMOTE HEPATIC REGENERATION OR TO REDUCE OR PREVENT HEPATOCYTE DEATH
WO2019158579A1 (en) 2018-02-13 2019-08-22 Vib Vzw Targeting minimal residual disease in cancer with rxr antagonists
CN112119072A (en) 2018-03-20 2020-12-22 普莱希科公司 Compounds and methods for IDO and TDO modulation, and indications thereof
MA52219A (en) 2018-03-30 2021-02-17 Incyte Corp TREATMENT OF SUPPURED HIDRADENITIS USING JAK INHIBITORS
CA3095934A1 (en) * 2018-04-05 2019-10-10 Beth Israel Deaconess Medical Center, Inc. Aryl hydrocarbon receptor modulators and uses thereof
EP3560516A1 (en) * 2018-04-27 2019-10-30 Bergen Teknologioverforing AS Combination therapy including beta-sitosterol in combination with at least one of a braf inhibitor, a mek inhibitor or an erk inhibitor and methods and use thereof
TW201946919A (en) 2018-05-17 2019-12-16 威斯塔研究所 EBNA1 inhibitor crystalline forms, and methods of preparing and using same
GB201808321D0 (en) * 2018-05-21 2018-07-11 Univ College Dublin Nat Univ Ireland Dublin Compositions and uses thereof
RU2687107C1 (en) * 2018-06-18 2019-05-07 федеральное государственное автономное образовательное учреждение высшего образования "Московский физико-технический институт (государственный университет)" Braf kinase inhibitor n-(3-(5-(4-chlorophenyl)-1h-pyrazolo[3,4-b]pyridin-3-carbonil)-2,4-difluorophenyl)propane-1-sulfonamide
EP3810139A1 (en) 2018-06-21 2021-04-28 HepaRegeniX GmbH Tricyclic protein kinase inhibitors for promoting liver regeneration or reducing or preventing hepatocyte death
EP4266058A3 (en) * 2018-07-06 2024-02-21 The Regents of the University of Colorado, a body corporate Genetically encoded system for constructing and detecting biologically active agents
BR112020026003A2 (en) 2018-07-16 2021-03-23 Heparegenix Gmbh protein kinase inhibitors to promote liver regeneration or reduce or prevent hepatocyte death
US20210253571A1 (en) * 2018-07-19 2021-08-19 Medshine Discovery Inc. Azaindole derivative and use thereof as fgfr and c-met inhibitor
KR102612513B1 (en) 2018-07-31 2023-12-12 록쏘 온콜로지, 인코포레이티드 SPRAY-DRIED DISPERSIONS AND FORMULATIONS OF (S)-5-AMINO-3-(4-((5-FLUORO-2-METHOXYBENZAMIDO)METHYL)PHENYL)-1-(1,1,1-TRIFLUORO PROPAN-2-YL)-lH-PYRAZOLE-4-CARBOXAMIDE
WO2020055672A1 (en) 2018-09-10 2020-03-19 Array Biopharma Inc. Fused heterocyclic compounds as ret kinase inhibitors
FI3860598T3 (en) 2018-10-03 2023-10-26 Jyvaeskylaen Yliopisto Vemurafenib and salts thereof for use in the treatment of enteroviral infections
US11066404B2 (en) 2018-10-11 2021-07-20 Incyte Corporation Dihydropyrido[2,3-d]pyrimidinone compounds as CDK2 inhibitors
WO2020079581A1 (en) 2018-10-16 2020-04-23 Novartis Ag Tumor mutation burden alone or in combination with immune markers as biomarkers for predicting response to targeted therapy
MX2021005171A (en) * 2018-11-16 2021-10-01 California Inst Of Techn Erk inhibitors and uses thereof.
CA3120371A1 (en) 2018-11-20 2020-05-28 Nflection Therapeutics, Inc. Cyanoaryl-aniline compounds for treatment of dermal disorders
EP3891136B1 (en) * 2018-12-03 2023-07-19 Merck Patent GmbH 4-heteroarylcarbonyl-n-(phenyl or heteroaryl)piperidine-1-carboxamides as inhibitors of tankyrases
US20220081438A1 (en) 2018-12-19 2022-03-17 Array Biopharma Inc. Substituted pyrazolo[1,5-a]pyridine compounds as inhibitors of fgfr tyrosine kinases
EP3898615A1 (en) 2018-12-19 2021-10-27 Array Biopharma, Inc. 7-((3,5-dimethoxyphenyl)amino)quinoxaline derivatives as fgfr inhibitors for treating cancer
US20220127254A1 (en) * 2019-02-11 2022-04-28 Merck Patent Gmbh Indazolyl-isoxazole derivatives for the treatment of diseases such as cancer
WO2020168197A1 (en) 2019-02-15 2020-08-20 Incyte Corporation Pyrrolo[2,3-d]pyrimidinone compounds as cdk2 inhibitors
US11472791B2 (en) 2019-03-05 2022-10-18 Incyte Corporation Pyrazolyl pyrimidinylamine compounds as CDK2 inhibitors
US11919904B2 (en) 2019-03-29 2024-03-05 Incyte Corporation Sulfonylamide compounds as CDK2 inhibitors
TW202104221A (en) 2019-04-09 2021-02-01 美商普雷辛肯公司 Compounds and methods for ep300 or cbp modulation and indications therefor
WO2020223558A1 (en) 2019-05-01 2020-11-05 Incyte Corporation Tricyclic amine compounds as cdk2 inhibitors
WO2020223469A1 (en) 2019-05-01 2020-11-05 Incyte Corporation N-(1-(methylsulfonyl)piperidin-4-yl)-4,5-di hydro-1h-imidazo[4,5-h]quinazolin-8-amine derivatives and related compounds as cyclin-dependent kinase 2 (cdk2) inhibitors for treating cancer
MA56047A (en) * 2019-05-29 2022-04-06 Ifm Due Inc COMPOUNDS AND COMPOSITIONS FOR THE TREATMENT OF DISEASES ASSOCIATED WITH STING ACTIVITY
CN114008042B (en) * 2019-05-31 2023-10-03 重庆复尚源创医药技术有限公司 Substituted pyrrolo [2,3-b ] pyridine and pyrazolo [3,4-b ] pyridine derivatives as protein kinase inhibitors
AU2020320001A1 (en) 2019-07-29 2022-03-10 Heparegenix Gmbh Heteroaryl-substituted pyrazolo-pyridine protein kinase inhibitors for promoting liver regeneration or reducing or preventing hepatocyte death
MX2022001940A (en) 2019-08-14 2022-05-10 Incyte Corp Imidazolyl pyrimidinylamine compounds as cdk2 inhibitors.
US20220347182A1 (en) * 2019-10-02 2022-11-03 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for the treatment of osteoarthritis
PE20221905A1 (en) 2019-10-11 2022-12-23 Incyte Corp BICYCLIC AMINES AS INHIBITORS OF CDK2
CN111004210A (en) * 2019-10-23 2020-04-14 中山大学 Compound single crystal and method for producing same
BR112022012110A2 (en) 2019-12-19 2022-12-13 Arvinas Operations Inc COMPOUNDS AND METHODS FOR TARGETED ANDROGEN RECEPTOR DEGRADATION
CA3161339A1 (en) 2019-12-27 2021-07-01 Schrodinger, Inc. Cyclic compounds and methods of using same
US20230090552A1 (en) 2020-01-08 2023-03-23 Synthis Therapeutics, Inc. Alk5 inhibitor conjugates and uses thereof
CN114981267A (en) 2020-01-15 2022-08-30 海帕瑞吉尼克斯股份有限公司 MKK4 inhibitor 3-benzoyl-1H-pyrrolo [2,3-B ] pyridine derivatives for the treatment of liver diseases
US20230090446A1 (en) 2020-01-28 2023-03-23 Universite De Strasbourg Antisense oligonucleotide targeting linc00518 for treating melanoma
EP4135700A1 (en) * 2020-04-15 2023-02-22 Pyramid Biosciences, Inc. Methods for preparing tyrosine receptor kinase inhibitors
TW202206422A (en) 2020-04-23 2022-02-16 美商普雷辛肯公司 Compounds and methods for cd73 modulation and indications therefor
US11833155B2 (en) 2020-06-03 2023-12-05 Incyte Corporation Combination therapy for treatment of myeloproliferative neoplasms
US11529335B2 (en) 2020-07-31 2022-12-20 University Of Iowa Research Foundation Compositions and methods for treating cancer
CA3192047A1 (en) 2020-08-21 2022-02-24 Plexxikon Inc. Combinational drug anticancer therapies
EP4211140A1 (en) 2020-09-10 2023-07-19 Schrödinger, Inc. Heterocyclic pericondensed cdc7 kinase inhibitors for the treatment of cancer
WO2022063101A1 (en) * 2020-09-23 2022-03-31 劲方医药科技(上海)有限公司 Aroyl substituted tricyclic compound, preparation method therefor and use thereof
CN114478528A (en) * 2020-10-28 2022-05-13 劲方医药科技(上海)有限公司 Aroyl-substituted tricyclic compound, preparation method and application thereof
TW202227437A (en) * 2020-11-17 2022-07-16 大陸商重慶復創醫藥研究有限公司 Substituted pyrrolo [2, 3-b] pyridine and pyrazolo [3,4-b] pyridine derivatives as protein kinase inhibitors
US20240002377A1 (en) * 2020-12-07 2024-01-04 Medshine Discovery Inc. Pyrrolopyridine compound and application thereof
EP4255420A1 (en) * 2020-12-07 2023-10-11 Lieber Institute, Inc. Compounds for inhibiting inositol hexakisphosphate kinase (ip6k) and methods of use thereof
JP2024500919A (en) 2020-12-23 2024-01-10 ジェンザイム・コーポレーション Deuterated colony stimulating factor-1 receptor (CSF-1R) inhibitor
JP2024505890A (en) 2021-01-26 2024-02-08 シュレーディンガー， インコーポレイテッド Tricyclic compounds useful in the treatment of cancer, autoimmune and inflammatory disorders
CN112574200B (en) * 2021-02-26 2021-06-11 安润医药科技（苏州）有限公司 Small molecule inhibitors of BTK and/or mutant C481S of BTK
CN115073469B (en) * 2021-03-15 2023-12-22 药雅科技(上海)有限公司 Preparation and application of pyrrolopyrimidine compound as kinase inhibitor
TW202300150A (en) 2021-03-18 2023-01-01 美商薛定諤公司 Cyclic compounds and methods of using same
WO2022195551A1 (en) 2021-03-18 2022-09-22 Novartis Ag Biomarkers for cancer and methods of use thereof
TW202304890A (en) * 2021-04-14 2023-02-01 美商百歐克斯製藥公司 Bicyclic heteroaromatic inhibitors of klk5
WO2023059786A1 (en) * 2021-10-06 2023-04-13 H. Lee Moffitt Cancer Center And Research Institute, Inc. Inhibitors of ulk1 and methods of use
WO2023174300A1 (en) * 2022-03-15 2023-09-21 劲方医药科技(上海)有限公司 Crystal form of btk inhibitor, acid salt thereof, and crystal form of acid salt thereof
WO2023178240A2 (en) * 2022-03-16 2023-09-21 The Children's Medical Center Corporation Pyk2 inhibition modulates immune cell function
WO2024003350A1 (en) 2022-06-30 2024-01-04 Universite De Strasbourg Combination therapy for melanoma
CN115141197B (en) * 2022-07-27 2024-03-26 安徽医科大学 3-aromatic heterocycle substituted phenyl derivative and preparation method and application thereof
WO2024033381A1 (en) 2022-08-10 2024-02-15 Vib Vzw Inhibition of tcf4/itf2 in the treatment of cancer

Family Cites Families (166)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3124610A (en) * 1964-03-10 Preparation of intermediates
US2234705A (en) * 1940-04-12 1941-03-11 Eastman Kodak Co Cellulose organic derivative composition containing esters of monoalkoxy benzoic acids
US2413258A (en) * 1942-07-07 1946-12-24 United Gas Improvement Co Polystyrene-type resins plasticized with high boiling fatty acid alkyl esters
US3067237A (en) * 1959-04-14 1962-12-04 Sterling Drug Inc Lower-alkyl mono-or bis-(alkanesulfonamido) benzoate esters
GB1141949A (en) 1966-02-23 1969-02-05 Sterling Drug Inc 7-azaindole derivatives
BR6794063D0 (en) * 1966-10-21 1973-09-18 Minnesota Mining & Mfg PROCESS TO PREPARE N-SUBSTITUTED PERFLUORAL-KILSULPHONAMIDES AND HERBICIDES AND PHYTOREGULATING COMPOSITIONS BASED ON THEM
DE2413258A1 (en) * 1974-03-20 1975-10-02 Bayer Ag Herbicidal N-(alkoxycarbonyl-phenyl)-N'-methyl-urea derivs - prepd by reacting alkoxycarbonyl-phenyl isocyanates with methylamines
AU7915675A (en) * 1974-03-20 1976-09-23 Bayer Ag Alkoxycarbonylphenylureas
GB1573212A (en) 1976-04-15 1980-08-20 Technicon Instr Immunoassay for gentamicin
US4301159A (en) * 1980-06-20 1981-11-17 Shionogi & Co., Ltd. N-(Diethylaminoethyl)-2-alkoxy-benzamide derivatives
AU547405B2 (en) * 1981-07-08 1985-10-17 Sanofi Amidobenzamides
US4664504A (en) 1983-01-20 1987-05-12 Tokyo Shibaura Denki Kabushiki Kaisha Image forming apparatus
US4568649A (en) * 1983-02-22 1986-02-04 Immunex Corporation Immediate ligand detection assay
US4626513A (en) 1983-11-10 1986-12-02 Massachusetts General Hospital Method and apparatus for ligand detection
DE3486287T2 (en) * 1983-11-14 1994-06-01 Dowelanco Intermediates for the preparation of 1,2,4-triazolo [1,5-a] pyrimidine-2-sulfonamides.
AU567140B2 (en) * 1984-01-06 1987-11-12 Shionogi & Co., Ltd. Sulphonamido-benzamide derivatives
ATE56096T1 (en) 1984-03-15 1990-09-15 Immunex Corp TEST FOR INSTANT DETECTION OF LIGANDS, TEST KIT AND ITS PREPARATION.
IT1196133B (en) * 1984-06-06 1988-11-10 Ausonia Farma Srl FURANIC DERIVATIVES WITH ANTI-ULCER ACTIVITY
US4714693A (en) 1986-04-03 1987-12-22 Uop Inc. Method of making a catalyst composition comprising uniform size metal components on carrier
US5688655A (en) * 1988-02-10 1997-11-18 Ict Pharmaceuticals, Inc. Method of screening for protein inhibitors and activators
US5700637A (en) * 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US6054270A (en) * 1988-05-03 2000-04-25 Oxford Gene Technology Limited Analying polynucleotide sequences
US5658775A (en) * 1988-05-17 1997-08-19 Sloan-Kettering Institute For Cancer Research Double copy retroviral vector
CA1339354C (en) * 1988-09-01 1997-08-26 The Whitehead Institute For Biomedical Research Recombinant retroviruses with amphotropic and ecotropic host ranges
US5703055A (en) * 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5527681A (en) * 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5800992A (en) * 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5360822A (en) * 1990-02-07 1994-11-01 Nippon Shinyaku Co. Ltd. Sulfonanilide derivatives and medicine
AU7906691A (en) * 1990-05-23 1991-12-10 United States of America, as represented by the Secretary, U.S. Department of Commerce, The Adeno-associated virus (aav)-based eucaryotic vectors
DE4022414A1 (en) * 1990-07-13 1992-01-16 Bayer Ag SUBSTITUTED PYRROLO-PYRIDINE
US5124335A (en) 1991-01-30 1992-06-23 Merck & Co., Inc. Substituted pyrollo-fused 6 membered heterocycles as angiotensin ii antagonists
JPH06509578A (en) * 1991-07-26 1994-10-27 ユニバーシティ・オブ・ロチェスター Cancer treatment using malignant cells
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
FR2687402B1 (en) * 1992-02-14 1995-06-30 Lipha NOVEL AZAINDOLES, METHODS OF PREPARATION AND MEDICAMENTS CONTAINING THEM.
JPH05236997A (en) * 1992-02-28 1993-09-17 Hitachi Ltd Chip for catching polynucleotide
US5576319A (en) 1993-03-01 1996-11-19 Merck, Sharp & Dohme Ltd. Pyrrolo-pyridine derivatives
ATE184008T1 (en) * 1993-03-01 1999-09-15 Merck Sharp & Dohme PYRROLOPYRIDINE DERIVATIVES AS DOPAMINE RECEPTOR LIGANDS
HUT71799A (en) * 1993-03-01 1996-02-28 Merck Sharp & Dohme Pyrrolo-pyridine derivatives, pharmaceutical compositions containing the same, process for producing them
US5549614A (en) * 1993-03-18 1996-08-27 Tunis; Scott W. Apparatus for folding flexible intraocular lenses
US5840485A (en) * 1993-05-27 1998-11-24 Selectide Corporation Topologically segregated, encoded solid phase libraries
EP0705279B1 (en) * 1993-05-27 2003-02-19 Selectide Corporation Topologically segregated, encoded solid phase libraries
US5631236A (en) * 1993-08-26 1997-05-20 Baylor College Of Medicine Gene therapy for solid tumors, using a DNA sequence encoding HSV-Tk or VZV-Tk
US5426039A (en) * 1993-09-08 1995-06-20 Bio-Rad Laboratories, Inc. Direct molecular cloning of primer extended DNA containing an alkane diol
JP3545461B2 (en) 1993-09-10 2004-07-21 エーザイ株式会社 Bicyclic heterocycle-containing sulfonamide derivatives
US6045996A (en) * 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US6468742B2 (en) * 1993-11-01 2002-10-22 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip
US5965452A (en) * 1996-07-09 1999-10-12 Nanogen, Inc. Multiplexed active biologic array
US5486525A (en) * 1993-12-16 1996-01-23 Abbott Laboratories Platelet activating factor antagonists: imidazopyridine indoles
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5763198A (en) * 1994-07-22 1998-06-09 Sugen, Inc. Screening assays for compounds
GB9416189D0 (en) 1994-08-10 1994-09-28 Merck Sharp & Dohme Therapeutic agents
GB9416162D0 (en) 1994-08-10 1994-09-28 Merck Sharp & Dohme Therapeutic agents
DE4430212A1 (en) * 1994-08-28 1996-02-29 Merck Patent Gmbh Ortho-substituted benzoic acid derivatives
US5556752A (en) * 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5830645A (en) * 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays
GB2298199A (en) * 1995-02-21 1996-08-28 Merck Sharp & Dohme Synthesis of azaindoles
GB9503400D0 (en) 1995-02-21 1995-04-12 Merck Sharp & Dohme Therpeutic agents
US5959098A (en) * 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
US6117681A (en) * 1995-03-29 2000-09-12 Bavarian Nordic Research Inst. A/S Pseudotyped retroviral particles
GB9507291D0 (en) 1995-04-07 1995-05-31 Merck Sharp & Dohme Therapeutic agents
GB2299581A (en) 1995-04-07 1996-10-09 Merck Sharp & Dohme 3-(Tetrahydropyridin-1-yl-methyl)pyrrolo[2,3-b]pyridine derivatives as ligands for dopamine receptor subtypes
US6110456A (en) * 1995-06-07 2000-08-29 Yale University Oral delivery or adeno-associated viral vectors
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5866411A (en) * 1995-09-08 1999-02-02 Pedersen; Finn Skou Retroviral vector, a replication system for said vector and avian or mammalian cells transfected with said vector
US5747276A (en) * 1995-09-15 1998-05-05 The Scripps Research Institute Screening methods for the identification of novel antibiotics
WO1997016533A1 (en) * 1995-10-31 1997-05-09 The Regents Of The University Of California Mammalian artificial chromosomes and methods of using same
US6022963A (en) * 1995-12-15 2000-02-08 Affymetrix, Inc. Synthesis of oligonucleotide arrays using photocleavable protecting groups
US6013440A (en) * 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US6025155A (en) * 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US5804585A (en) * 1996-04-15 1998-09-08 Texas Biotechnology Corporation Thieno-pyridine sulfonamides derivatives thereof and related compounds that modulate the activity of endothelin
CA2271767A1 (en) * 1996-11-19 1998-05-28 Nathan B. Mantlo Aryl and heteroaryl substituted fused pyrrole antiinflammatory agents
US6294330B1 (en) * 1997-01-31 2001-09-25 Odyssey Pharmaceuticals Inc. Protein fragment complementation assays for the detection of biological or drug interactions
WO1998039471A1 (en) * 1997-03-07 1998-09-11 Tropix, Inc. Protease inhibtor assay
US5977131A (en) 1997-04-09 1999-11-02 Pfizer Inc. Azaindole-ethylamine derivatives as nicotinic acetylcholine receptor binding agents
US6096718A (en) * 1997-06-05 2000-08-01 Gene Targeting Corp. Tissue specific adenovirus vectors for breast cancer treatment
BR9810456A (en) 1997-06-27 2001-09-25 Fujisawa Pharmaceutical Co Sulfonamide compound, method for obtaining it and its pharmaceutical use
US6235769B1 (en) * 1997-07-03 2001-05-22 Sugen, Inc. Methods of preventing and treating neurological disorders with compounds that modulate the function of the C-RET receptor protein tyrosine kinase
US6826296B2 (en) * 1997-07-25 2004-11-30 Affymetrix, Inc. Method and system for providing a probe array chip design database
US6161776A (en) * 1997-08-12 2000-12-19 Nibco Inc. Multi-layered, porous mat turf irrigation apparatus and method
PT1012564E (en) * 1997-09-11 2003-08-29 Bioventures Inc METHOD OF PRODUCTION OF HIGH DENSITY ARRANGEMENTS
US6178384B1 (en) * 1997-09-29 2001-01-23 The Trustees Of Columbia University In The City Of New York Method and apparatus for selecting a molecule based on conformational free energy
US6465178B2 (en) * 1997-09-30 2002-10-15 Surmodics, Inc. Target molecule attachment to surfaces
US6048695A (en) * 1998-05-04 2000-04-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
WO1999063929A2 (en) * 1998-06-08 1999-12-16 Advanced Medicine, Inc. Multibinding inhibitors of microsomal triglyceride transferase protein
US6113913A (en) * 1998-06-26 2000-09-05 Genvec, Inc. Recombinant adenovirus
US6184226B1 (en) 1998-08-28 2001-02-06 Scios Inc. Quinazoline derivatives as inhibitors of P-38 α
IL141724A0 (en) 1998-08-28 2002-03-10 Scios Inc INHIBITORS OF P38-α KINASE
US6350786B1 (en) 1998-09-22 2002-02-26 Hoffmann-La Roche Inc. Stable complexes of poorly soluble compounds in ionic polymers
US6277628B1 (en) * 1998-10-02 2001-08-21 Incyte Genomics, Inc. Linear microarrays
US6277489B1 (en) * 1998-12-04 2001-08-21 The Regents Of The University Of California Support for high performance affinity chromatography and other uses
US20010001449A1 (en) 1998-12-30 2001-05-24 Thomas R. Kiliany Low-pressure hydrocracking process
TR200103336T2 (en) * 1999-03-17 2002-04-22 Astrazeneca Ab Amide derivatives.
GB9906566D0 (en) 1999-03-23 1999-05-19 Zeneca Ltd Chemical compounds
US6221653B1 (en) * 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
FR2793793B1 (en) 1999-05-19 2004-02-27 Adir NOVEL SUBSTITUTED DIMERIC DERIVATIVES, PROCESS FOR PREPARING THEM AND PHARMACEUTICAL COMPOSITIONS CONTAINING SAME
TWI234557B (en) * 1999-05-26 2005-06-21 Telik Inc Novel naphthalene ureas as glucose uptake enhancers
US6653151B2 (en) * 1999-07-30 2003-11-25 Large Scale Proteomics Corporation Dry deposition of materials for microarrays using matrix displacement
US20010008765A1 (en) * 1999-12-06 2001-07-19 Fuji Photo Film Co., Ltd. DNA chip and reactive solid carrier
NZ519697A (en) * 1999-12-22 2004-08-27 Sugen Inc Methods of modulating c-kit tyrosine protein kinase function with indolinone compounds
NZ519380A (en) 1999-12-28 2004-10-29 Eisai Co Ltd Heterocyclic compounds having sulfonamide groups
JP2001278886A (en) * 2000-03-28 2001-10-10 Dai Ichi Seiyaku Co Ltd Benzoxazine derivative and medicament containing the same
GB0007934D0 (en) * 2000-03-31 2000-05-17 Darwin Discovery Ltd Chemical compounds
CN101025419A (en) 2001-02-21 2007-08-29 卫材R&D管理有限公司 Method for testing effect of angiogenesis inhibitor via integrin expression inhibition
US7109208B2 (en) 2001-04-11 2006-09-19 Senju Pharmaceutical Co., Ltd. Visual function disorder improving agents
EP1381600A1 (en) 2001-04-24 2004-01-21 Wyeth, A Corporation of the State of Delaware Antidepressant azaheterocyclylmethyl derivatives of 2,3-dihydro-1,4-benzodioxan
GB0114417D0 (en) * 2001-06-13 2001-08-08 Boc Group Plc Lubricating systems for regenerative vacuum pumps
WO2002102783A1 (en) 2001-06-19 2002-12-27 Merck & Co., Inc. Tyrosine kinase inhibitors
US7291639B2 (en) 2001-06-20 2007-11-06 Wyeth Aryloxy-acetic acid compounds useful as inhibitors of plasminogen activator inhibitor-1 (PAI-1)
GB0115109D0 (en) * 2001-06-21 2001-08-15 Aventis Pharma Ltd Chemical compounds
UA75425C2 (en) 2001-07-09 2006-04-17 Piperazine oxime derivatives with antagonistic activity to nk-1 receptor, use thereof, a pharmaceutical composition based thereon, a method for producing and a method for producing intermediary compounds
GB0117583D0 (en) 2001-07-19 2001-09-12 Astrazeneca Ab Novel compounds
US6858860B2 (en) * 2001-07-24 2005-02-22 Seiko Epson Corporation Apparatus and method for measuring natural period of liquid
GB0118479D0 (en) 2001-07-28 2001-09-19 Astrazeneca Ab Novel compounds
WO2003028724A1 (en) 2001-10-04 2003-04-10 Smithkline Beecham Corporation Chk1 kinase inhibitors
JPWO2003037862A1 (en) 2001-10-30 2005-02-17 日本新薬株式会社 Amide derivatives and pharmaceuticals
US20030236277A1 (en) 2002-02-14 2003-12-25 Kadow John F. Indole, azaindole and related heterocyclic pyrrolidine derivatives
US6884889B2 (en) 2002-03-25 2005-04-26 Bristol-Myers Squibb Co. Processes for the preparation of antiviral 7-azaindole derivatives
EP1490364B1 (en) 2002-03-28 2007-09-26 Eisai R&D Management Co., Ltd. 7-azaindoles as inhibitors of c-jun n-terminal kinases for the treatment of neurodegenerative disorders
AU2003214414B2 (en) 2002-03-28 2008-10-09 Eisai R & D Management Co., Ltd. Azaindoles as inhibitors of c-Jun N-terminal kinases
GB0212785D0 (en) * 2002-05-31 2002-07-10 Glaxo Group Ltd Compounds
UA78999C2 (en) 2002-06-04 2007-05-10 Wyeth Corp 1-(aminoalkyl)-3-sulfonylazaindoles as ligands of 5-hydroxytryptamine-6
TW200403243A (en) 2002-07-18 2004-03-01 Wyeth Corp 1-Heterocyclylalkyl-3-sulfonylazaindole or-azaindazole derivatives as 5-hydroxytryptamine-6 ligands
TWI329112B (en) 2002-07-19 2010-08-21 Bristol Myers Squibb Co Novel inhibitors of kinases
US6878887B2 (en) * 2002-08-07 2005-04-12 Matsushita Electric Industrial Co., Ltd. Anti-malfunction mechanism for variable output device
SE0202463D0 (en) 2002-08-14 2002-08-14 Astrazeneca Ab Novel compounds
BRPI0314042B8 (en) * 2002-09-06 2021-05-25 Calando Pharmaceuticals Inc cyclodextrin-based polymers for the delivery of covalently linked therapeutic agents
CA2503905A1 (en) 2002-09-16 2004-03-25 Plexxikon, Inc. Crystal structure of pim-1 kinase
US7645771B2 (en) * 2002-12-13 2010-01-12 Smithkline Beecham Corp. CCR5 antagonists as therapeutic agents
ES2311755T3 (en) * 2002-12-13 2009-02-16 Smithkline Beecham Corporation CYCLHEXYL COMPOUNDS AS CCRS ANTAGONISTS.
US7696225B2 (en) * 2003-01-06 2010-04-13 Osi Pharmaceuticals, Inc. (2-carboxamido)(3-Amino) thiophene compounds
SE0300120D0 (en) 2003-01-17 2003-01-17 Astrazeneca Ab Novel compounds
SE0300119D0 (en) 2003-01-17 2003-01-17 Astrazeneca Ab Novel compounds
US20050085463A1 (en) * 2003-01-23 2005-04-21 Weiner David M. Use of N-desmethylclozapine to treat human neuropsychiatric disease
BRPI0407493A (en) * 2003-02-14 2006-02-14 Wyeth Corp heterocyclyl-3-sulfinylazaindole or -azaindazole derivatives as 5-hydroxytryptamine-6 binders
JP2007524374A (en) 2003-02-28 2007-08-30 プレキシコン，インコーポレーテッド PYK2 crystal structure and use
US20050164300A1 (en) 2003-09-15 2005-07-28 Plexxikon, Inc. Molecular scaffolds for kinase ligand development
DE10357510A1 (en) 2003-12-09 2005-07-07 Bayer Healthcare Ag Heteroaryl-substituted benzenes
SI1696920T1 (en) * 2003-12-19 2015-02-27 Plexxikon Inc. Compounds and methods for development of ret modulators
DK2119771T3 (en) 2003-12-24 2018-12-10 Dupont Nutrition Biosci Aps PROTEINS
GB0330043D0 (en) * 2003-12-24 2004-01-28 Pharmacia Italia Spa Pyrrolo [2,3-b] pyridine derivatives active as kinase inhibitors process for their preparation and pharmaceutical compositions comprising them
GB0330042D0 (en) 2003-12-24 2004-01-28 Pharmacia Italia Spa Pyrrolo [2,3-b] pyridine derivatives active as kinase inhibitors process for their preparation and pharmaceutical compositions them
GB0403635D0 (en) 2004-02-18 2004-03-24 Devgen Nv Pyridinocarboxamides with improved activity as kinase inhibitors
GB0405055D0 (en) 2004-03-05 2004-04-07 Eisai London Res Lab Ltd JNK inhibitors
RS51830B (en) 2004-03-30 2012-02-29 Vertex Pharmaceuticals Incorporated Azaindoles useful as inhibitors of jak and other protein kinases
US7498342B2 (en) * 2004-06-17 2009-03-03 Plexxikon, Inc. Compounds modulating c-kit activity
WO2006009797A1 (en) 2004-06-17 2006-01-26 Plexxikon, Inc. Azaindoles modulating c-kit activity and uses therefor
AU2005260689B2 (en) 2004-06-30 2012-05-10 Vertex Pharmaceuticals Incorporated Azaindoles useful as inhibitors of protein kinases
SG156685A1 (en) * 2004-07-27 2009-11-26 Sgx Pharmaceuticals Inc Fused ring heterocycle kinase modulators
US7361764B2 (en) * 2004-07-27 2008-04-22 Sgx Pharmaceuticals, Inc. Pyrrolo-pyridine kinase modulators
US7709645B2 (en) * 2004-07-27 2010-05-04 Sgx Pharmaceuticals, Inc. Pyrrolo-pyridine kinase modulators
US7626021B2 (en) * 2004-07-27 2009-12-01 Sgx Pharmaceuticals, Inc. Fused ring heterocycle kinase modulators
EP1781654A1 (en) * 2004-07-27 2007-05-09 SGX Pharmaceuticals, Inc. Pyrrolo-pyridine kinase modulators
GB0419192D0 (en) * 2004-08-27 2004-09-29 Merck Sharp & Dohme Therapeutic agents
US8859581B2 (en) 2005-04-25 2014-10-14 Merck Patent Gmbh Azaheterocyclic compounds as kinase inhibitors
KR20080027775A (en) * 2005-05-17 2008-03-28 플렉시콘, 인코퍼레이티드 Pyrrole(2,3-b)pyridine derivatives protein kinase inhibitors
ZA200710379B (en) 2005-05-20 2009-05-27 Vertex Pharma Pyrrolopyridines useful as inhibitors of protein kinase
US7863288B2 (en) * 2005-06-22 2011-01-04 Plexxikon, Inc. Compounds and methods for kinase modulation, and indications therefor
GB0516156D0 (en) 2005-08-05 2005-09-14 Eisai London Res Lab Ltd JNK inhibitors
TW200812611A (en) 2006-03-20 2008-03-16 Vertex Pharma Pharmaceutical compositions
WO2008063888A2 (en) 2006-11-22 2008-05-29 Plexxikon, Inc. Compounds modulating c-fms and/or c-kit activity and uses therefor
PE20081581A1 (en) * 2006-12-21 2008-11-12 Plexxikon Inc PIRROLO [2,3-b] PYRIDINES COMPOUNDS AS KINASE MODULATORS
WO2008079909A1 (en) * 2006-12-21 2008-07-03 Plexxikon, Inc. Pyrrolo [2,3-b] pyridines as kinase modulators
TW200908968A (en) * 2007-05-29 2009-03-01 Sgx Pharmaceuticals Inc Substituted pyrrolopyridines and pyrazolopyridines as kinase modulators
TW201041888A (en) * 2009-05-06 2010-12-01 Plexxikon Inc Compounds and methods for kinase modulation, and indications therefor

Also Published As

Publication number Publication date
SI1893612T1 (en) 2012-03-30
PL1893612T3 (en) 2012-01-31
TW201514140A (en) 2015-04-16
RU2011101140A (en) 2012-07-20
ZA200711152B (en) 2012-06-27
BRPI0611863A2 (en) 2008-12-02
CA2613015A1 (en) 2007-01-04
NO2014006I1 (en) 2014-06-02
WO2007002433A1 (en) 2007-01-04
HK1164866A1 (en) 2012-09-28
US8415469B2 (en) 2013-04-09
RU2008100933A (en) 2009-07-27
LU92035I9 (en) 2019-01-04
US8470818B2 (en) 2013-06-25
CN102603581A (en) 2012-07-25
US8143271B2 (en) 2012-03-27
IL188248A (en) 2012-04-30
EP2395004B1 (en) 2016-01-20
ATE518860T1 (en) 2011-08-15
TWI522337B (en) 2016-02-21
PE20070100A1 (en) 2007-03-10
TW200804370A (en) 2008-01-16
KR101125919B1 (en) 2012-06-12
TW201345906A (en) 2013-11-16
US20180111929A1 (en) 2018-04-26
US20120053177A1 (en) 2012-03-01
JP5007304B2 (en) 2012-08-22
TWI473808B (en) 2015-02-21
MX2007016463A (en) 2008-03-04
MY153898A (en) 2015-04-15
CR20130216A (en) 2013-07-02
NO333913B1 (en) 2013-10-21
US20100256365A1 (en) 2010-10-07
RS52010B (en) 2012-04-30
CN101243084A (en) 2008-08-13
US20160176865A1 (en) 2016-06-23
RU2012150759A (en) 2014-06-10
AR054624A1 (en) 2007-07-04
CY2012021I2 (en) 2015-08-05
EP1893612B1 (en) 2011-08-03
EP2395004A2 (en) 2011-12-14
US20150290205A1 (en) 2015-10-15
CN102603581B (en) 2015-06-24
RU2629999C2 (en) 2017-09-05
JP2008546797A (en) 2008-12-25
RU2418800C2 (en) 2011-05-20
MA29612B1 (en) 2008-07-01
US7863288B2 (en) 2011-01-04
ES2371397T3 (en) 2011-12-30
US20120022098A1 (en) 2012-01-26
CN102206216A (en) 2011-10-05
CY1111996T1 (en) 2015-08-05
TWI432193B (en) 2014-04-01
NZ565255A (en) 2010-04-30
RU2565071C2 (en) 2015-10-20
AR078519A2 (en) 2011-11-16
UA95244C2 (en) 2011-07-25
PL2395004T3 (en) 2016-08-31
US20130261117A1 (en) 2013-10-03
MY147410A (en) 2012-11-30
DK2395004T3 (en) 2016-03-21
CY2012021I1 (en) 2015-08-05
AU2006261993B2 (en) 2011-11-17
HUE027370T2 (en) 2016-10-28
IL188248A0 (en) 2008-04-13
US20100249118A1 (en) 2010-09-30
EP1893612A1 (en) 2008-03-05
CN101243084B (en) 2012-03-07
HK1114610A1 (en) 2008-11-07
WO2007002325A1 (en) 2007-01-04
AU2006261993A1 (en) 2007-01-04
US20130303534A1 (en) 2013-11-14
ZA201202025B (en) 2022-03-30
ZA201202024B (en) 2016-02-24
EP3088400A1 (en) 2016-11-02
HUS1200009I1 (en) 2018-09-28
NO20076659L (en) 2008-03-18
NO2014006I2 (en) 2014-03-20
CN102206216B (en) 2014-11-12
DE122012000043I1 (en) 2012-09-13
PT1893612E (en) 2011-11-21
ZA201202026B (en) 2012-11-28
CR9677A (en) 2008-03-26
US20110059963A1 (en) 2011-03-10
LU92035I2 (en) 2012-08-28
DK1893612T3 (en) 2011-11-21
ECSP088121A (en) 2008-02-20
ES2565992T3 (en) 2016-04-08
EP2395004A3 (en) 2012-04-04
SI2395004T1 (en) 2016-05-31
KR20080030619A (en) 2008-04-04

Similar Documents

Publication Publication Date Title
CA2613015C (en) Pyrrolo [2, 3-b] pyridine derivatives as protein kinase inhibitors
US7846941B2 (en) Compounds modulating c-kit and c-fms activity and uses therefor
US7872018B2 (en) Compounds and methods for kinase modulation, and indications therefor
US20080188514A1 (en) Compounds and methods for kinase modulation, and indications therefor
AU2012200933B2 (en) Pyrrolo [2, 3-B] pyridine derivatives as protein kinase inhibitors
AU2015207857B2 (en) Pyrrolo [2,3-B] pyridine derivatives as protein kinase inhibitors

Legal Events

Date Code Title Description
EEER Examination request