CA2611863A1 - Target substance-capturing body, device for capturing target substance, and instrument, kit and method for detecting target substance by use of them - Google Patents

Target substance-capturing body, device for capturing target substance, and instrument, kit and method for detecting target substance by use of them Download PDF

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Publication number
CA2611863A1
CA2611863A1 CA002611863A CA2611863A CA2611863A1 CA 2611863 A1 CA2611863 A1 CA 2611863A1 CA 002611863 A CA002611863 A CA 002611863A CA 2611863 A CA2611863 A CA 2611863A CA 2611863 A1 CA2611863 A1 CA 2611863A1
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CA
Canada
Prior art keywords
target substance
capturing
substrate
capturing body
functional domain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002611863A
Other languages
French (fr)
Other versions
CA2611863C (en
Inventor
Hidenori Shiotsuka
Takeshi Imamura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Canon Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2611863A1 publication Critical patent/CA2611863A1/en
Application granted granted Critical
Publication of CA2611863C publication Critical patent/CA2611863C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

Abstract

It is intended to provide a target substance-capturing body comprising: a base consisting of a soluble protein; and two or more functional domains capable of binding to different target substances.

Claims (15)

1. A target substance-capturing body characterized by comprising: a base consisting of a soluble protein; and two or more functional domains respectively capable of binding to different target substances.
2. The target substance-capturing body according to claim 1, wherein the base has at least n types of soluble proteins corresponding to the types (n: n>=2) of the functional domains, and the target substance-capturing body is a protein complex composed of fusion proteins comprising the functional domains respectively fused with the different soluble proteins.
3. The target substance-capturing body according to claim 1, wherein the functional domain is composed of at least a portion of an antibody.
4. The target substance-capturing body according to claim 1, wherein the soluble protein is producible in a host cell and has a signal peptide necessary to be secreted out of cytoplasm when produced in the host.
5. The target substance-capturing body according to claim 1, wherein the soluble protein is a protein consisting of two or more constituent units.
6. The target substance-capturing body according to claim 5, wherein the functional domains are respectively fused with the different constituent units.
7. The target substance-capturing body according to claim 4, wherein the soluble protein is selected from among phage coat proteins.
8. The target substance-capturing body according to claim 1, wherein the two or more functional domains consist of a functional domain capable of binding to a substrate and a functional domain for capturing a target substance different from the substrate.
9. An device for capturing a target substance characterized by comprising a substrate and a target substance-capturing body according to claim 8, wherein the substrate is bound with the functional domain adapted for the substrate, and the functional domain for capturing the target substance different from the substrate maintains its capturing function.
10. The device according to claim 9, wherein at least a portion of the substrate contains gold, and the portion consisting of the gold is bound with the functional domain adapted for the substrate.
11. The device according to claim 9, wherein the functional domain except for the functional domain adapted for the substrate is intended to capture a target substance to be detected.
12. A detection instrument for detecting a target substance to be detected contained in an analyte characterized by comprising:

a device according to claim 11; and detection means for detecting the binding of a target substance to a functional domain for capturing a target substance to be detected comprised in the device.
13. A kit for detecting a target substance to be detected contained in an analyte characterized by comprising:

an device according to claim 11; and detection means for detecting the binding of a target substance to a functional domain for capturing a target substance to be detected comprised in the device.
14. A method for detecting a target substance to be detected in an analyte characterized by comprising the steps of:

reacting an device according to claim 11 with a target substance to be detected; and detecting the binding of the target substance to be detected to a functional domain of the device.
15. A detecting method according to claim 14, further comprising the step of preparing the device by reacting a target substance-capturing body according to claim 8 with a substrate to bind the target substance-capturing body to the substrate.
CA2611863A 2005-06-30 2006-06-27 Target substance-capturing body, device for capturing target substance, and instrument, kit and method for detecting target substance by use of them Expired - Fee Related CA2611863C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2005-192084 2005-06-30
JP2005192084A JP5142458B2 (en) 2005-06-30 2005-06-30 Target substance capture molecule, target substance capture element, target substance detection apparatus and kit using these, and target substance detection method
PCT/JP2006/313195 WO2007004600A2 (en) 2005-06-30 2006-06-27 Target substance-capturing body, device for capturing target substance, and instrument, kit and method for detecting target substance by use of them

Publications (2)

Publication Number Publication Date
CA2611863A1 true CA2611863A1 (en) 2007-01-11
CA2611863C CA2611863C (en) 2012-08-07

Family

ID=37496536

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2611863A Expired - Fee Related CA2611863C (en) 2005-06-30 2006-06-27 Target substance-capturing body, device for capturing target substance, and instrument, kit and method for detecting target substance by use of them

Country Status (5)

Country Link
US (1) US7700374B2 (en)
EP (1) EP1899729A2 (en)
JP (1) JP5142458B2 (en)
CA (1) CA2611863C (en)
WO (1) WO2007004600A2 (en)

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DE102007056818A1 (en) * 2007-11-23 2009-05-28 Bayer Technology Services Gmbh Bacteriophages and coating material for surfaces
CA3022196A1 (en) * 2008-07-16 2010-01-21 Institute For Research In Biomedicine Human cytomegalovirus neutralizing antibodies and use thereof

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Also Published As

Publication number Publication date
EP1899729A2 (en) 2008-03-19
US20080206784A1 (en) 2008-08-28
JP2007006789A (en) 2007-01-18
JP5142458B2 (en) 2013-02-13
WO2007004600A3 (en) 2007-06-07
WO2007004600A2 (en) 2007-01-11
US7700374B2 (en) 2010-04-20
CA2611863C (en) 2012-08-07

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Effective date: 20220627