CA2519528C - Antibodies against t cell immunoglobulin domain and mucin domain 1 (tim-1) antigen and uses thereof - Google Patents

Antibodies against t cell immunoglobulin domain and mucin domain 1 (tim-1) antigen and uses thereof Download PDF

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CA2519528C
CA2519528C CA2519528A CA2519528A CA2519528C CA 2519528 C CA2519528 C CA 2519528C CA 2519528 A CA2519528 A CA 2519528A CA 2519528 A CA2519528 A CA 2519528A CA 2519528 C CA2519528 C CA 2519528C
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amino acid
acid sequence
sequence
light chain
heavy chain
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CA2519528A1 (en
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Gregory M. Landes
Francine Chen
Binyam Bezabeh
Ian Foltz
Kam Fai Tse
Michael Jeffers
Mehdi Mesri
Gary Starling
Peter Mezes
Nikolia Khramtsov
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Amgen Fremont Inc
Celldex Therapeutics Inc
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Celldex Therapeutics Inc
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    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00Preparations containing radioactive substances for use in therapy or testing in vivo
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    • A61K51/04Organic compounds
    • A61K51/08Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/10Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
    • A61K51/1027Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody against receptors, cell-surface antigens or cell-surface determinants
    • A61K51/1039Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody against receptors, cell-surface antigens or cell-surface determinants against T-cell receptors
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00Drugs for disorders of the urinary system
    • A61P13/08Drugs for disorders of the urinary system of the prostate
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61P13/00Drugs for disorders of the urinary system
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00Drugs for disorders of the urinary system
    • A61P13/12Drugs for disorders of the urinary system of the kidneys
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
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    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • C07K2317/00Immunoglobulins specific features
    • C07K2317/20Immunoglobulins specific features characterized by taxonomic origin
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    • C07K2317/00Immunoglobulins specific features
    • C07K2317/60Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622Single chain antibody (scFv)
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    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/70Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
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    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
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    • C07K2317/76Antagonist effect on antigen, e.g. neutralization or inhibition of binding
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    • C07KPEPTIDES
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    • C07K2317/90Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3

Abstract

The invention described herein is related to antibodies directed to the antigen TIM-1 and uses of such antibodies. In particular, there are provided fully human monoclonal antibodies directed to the antigen TIM-1. Isolated polynucleotide sequences encoding, and amino acid sequences comprising, heavy and light chain immunoglobulin molecules, particularly sequences corresponding to contiguous heavy and light chain sequences spanning the framework regions (FR's) and/or complementarity determining regions (CDR's), specifically from FR1 through FR4 or CDR1 through CDR3, are provided. Hybridomas or other cell lines expressing such immunoglobulin molecules and monoclonal antibodies are also provided.

Description

ANTIBODIES AGAINST T CELL IM.MUNOGLOBULIN DOMAIN AND MUCIN
DOMAIN 1 (TIM-1) ANTIGEN AND USES THEREOF
Background of the Invention Field of the Invention
[0002] The invention disclosed herein is related to antibodies directed to the antigen T cell, immunoglobulin domain and mucin domain 1 (TIM-1) proteins and uses of such antibodies. In particular, there are provided fully human monoclonal antibodies directed to the antigen TIM-1. Nucleotide sequences encoding, and amino acid sequences comprising, heavy and light chain immunoglobulin molecules, particularly sequences corresponding to contiguous heavy and light chain sequences spanning the framework -regions and/or complementarity determining regions (CDRs), specifically from FR1 through FR4 or CDR1 through CDR3, are provided. Hybiidomas or other cell lines expressing such immunoglobulin molecules and monoclonal antibodies are also provided.
Description of the Related Art
[0003] A new family of genes encoding T cell, immunoglobulin domain and mucin domain (TIM) proteins (three in humans and eight in mice) have been described recently with emerging roles in immunity. Kuchroo et al., Nat Rev Immunol 3:454-462 (2003); McIntire et al., Nat Immunol 2:1109-1116 (2001). The TIM gene family members reside in chromosomal regions, 5q33.2 in human and 11B1.1 in mouse, and have been linked to allergy and autoimmune diseases. Shevach, Nat Rev Immunol 2:389-400 (2002);
Wills-Karp et al., Nat Immunol 4:1050-1052 (2003).
[0004] One TIM family member, TIM-1, is also known as Hepatitis A
virus cellular receptor (HAVcr-1) and was originally discovered as a receptor for Hepatitis A virus (HAV) (Kaplan et al, EMBO J15(16):4282-96 (1996)). This gene was later cloned as kidney injury molecule 1 (KIM-1) (Ichimura et al., J Biol Chem 273:4135-4142 (1998);
Han et al., Kidney Int 62:237-244 (2002)).
[0005] Kaplan et al. isolated the cellular receptor for hepatitis A
virus from a cDNA library from a primary African Green Monkey Kidney (AGMK) cell line expressing the receptor. See U.S. Patent No. 5,622,861. The disclosed utility of the polypeptides and nucleic acids was to diagnose infection by hepatitis A virus, to separate hepatitis A virus from impurities in a sample, to treat infection as well as to prevent infection by hepatitis A
virus. Furthermore, the polypeptides could be expressed in transformed cells and used to test efficacy of compounds in an anti-hepatitis A virus binding assay.
[0006] The human homolog, hHAVcr-1 (aka TIM-1), was described by Feigelstock et al., J Virology 72(8): 6621-6628 (1998). The same molecules were described in PCT Publication Nos: WO 97/44460 and WO 98/53071 and U.S. Patent No.
6,664,385 as Kidney Injury-related Molecules (KIM) that were found to be upregulated in renal tissue after injury to the kidney. The molecules were described as being useful in a variety of therapeutic interventions, specifically, renal disease, disorder or injury. For example, PCT Publication No. WO 02/098920 describes antibodies to KIM and describes antibodies that inhibit the shedding of KIM-1 polypeptide from KIM-1 expressing cells e.g., renal cells, or renal cancer cells.
[0007] TIM-1 is a type 1 membrane protein that contains a novel six-cysteine immunoglobulin-like domain and a mucin threonine/serine.proline-rich (T/S/P) domain.
TIM-1 was originally identified in rat. TIM-1 has been found in mouse, African green monkey, and humans (Feigelstock et al., J Virol 72(8):6621-8 (1998). The African green monkey ortholog is most closely related to human TIM-1 showing 77.6% amino acid identity over 358 aligned amino acids. Rat and mouse orthologs exhibit 50% (155/310) and 45.6%
(126/276) amino acid identity respectively, although over shorter segments of aligned sequence than for African green monkey. Monoclonal antibodies to the Ig-like domain of TIM-1 have been shown to be protective against Hepatitis A Virus infection in vitro.

Silberstein et al., J Virol 75(2):717-25 (2001). In addition, Kim-1 was shown to be expressed at low levels in normal kidney but its expression is increased dramatically in postischemic kidney. Ichimura et al., J Biol Chem 273(7):4135-42 (1998). HAVCR-1 is also expressed at elevated levels in clear cell carcinomas and cancer cell lines derived from the same.
[0000] TIM-1 shows homology to the P-type "trefoil" domain suggesting that it may have similar biological activity to other P-type trefoil family members.
Some trefoil domain containing proteins have been shown to induce cellular scattering and invasion when used to treat kidney, colon and breast tumor cell lines. Prest et al., FASEB J
16(6):592-4 (2002). In addition, some trefoil containing proteins confer cellular resistance to anoikis, an anchorage-related apoptosis phenomenon in epithelium. Chen et al., Biochem Biophys Res COMMUll 274(3):576-82 (2000).
[0009] TIM-1 maps to a region of human chromosome 5 known as Tapr in the murine sytenic region that has been implicated in asthma. Tapr, a major T cell regulatory, locus, controls the development of airway hyperreactivity. Wills-Karp, Nature Immunology 2:1095-1096 (2001); McIntire et al., Nature Immunology 2:1109-1116 (2001).
Summary of the Invention [0010] Embodiments of the invention described herein are based upon the development of human monoclonal antibodies, or binding fragements thereof, that bind TIM-1 and affect TIM-1 function. TIM-1 is expressed at elevated levels in pathologies, such as neoplasms and inflammatory diseases. Inhibition of the biological activity of TIM-1 can thus prevent inflammation and other desired effects, including TIM-1 induced cell proliferation.
Embodiments of the invention are based upon the generation and identification of isolated antibodies, or binding fragments thereof, that bind specifically to TIM-1.
[0011] Accordingly, one embodiment of the invention includes isolated antibodies, or fragments of those antibodies, that specifically bind to TIM-1.
As known in the art, the antibodies can advantageously be, for example, monoclonal, chimeric and/or fully human antibodies. Embodiments of the invention described herein also provide cells for producing these antibodies.

[0012] Some embodiments of the invention described herein relate to monoclonal antibodies that bind TIM-1 and affect TIM-1 function. Other embodiments relate to fully human anti-TIM-1 antibodies and anti-TIM-1 antibody preparations with desirable properties from a therapeutic perspective, including strong binding affinity for TIM-1, the ability to neutralize TIM-1 in vitro and in vivo, and the ability to inhibit TIM-1 induced cell proliferation.
[0013] In a preferred embodiment, antibodies described herein bind to with very high affinities (Kd). For example a human, rabbit, mouse, chimeric or humanized antibody that is capable of binding TIM-1 with a Kd less than, but not limited to, 1 e, 10-8, 100, 10,1, 1012, 10-13 or 10-14 M, or any range or value therein. Affinity and/or avidity measurements can be measured by KinExA and/or BIACORE , as described herein.
[0014] In one embodiment, the invention provides an isolated antibody that specifically binds to T cell, immunoglobulin domain and mucin domain 1 (TIM-1). In some embodiments, the isolated antibody has a heavy chain polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 6, 10, 14, 18, 22, 26, 30, 34, 38, 42, 46, and 50.
[0015] In another embodiment, the invention provides an isolated antibody that specifically binds to T cell, immunoglobulin domain and mucin domain 1 (TI1\4-1) and has a light chain polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, 48, and 52.
[0016] In yet another embodiment, the invention provides an isolated antibody that specifically binds to TIM-1 and has a heavy chain polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 6, 10, 14, 18, 22, 26, 30, 34, 38, 42, 46, and 50 and has a light chain polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, 48, and 52.
[0017] Another embodiment of the invention is a fully human antibody that specifically binds to TIM-1 and has a heavy chain polypeptide comprising an amino acid sequence comprising the complementarity determining region (CDR) with one of the sequences shown in Table 4. It is noted that CDR determinations can be readily accomplished by those of ordinary skill in the art. See for example, Kabat et al., Sequences of Proteins of Immunological Interest, Fifth Edition, NIEI Publication 91-3242, Bethesda MD
[1991], vols. 1-3.
[0013] Yet another embodiment is an antibody that specifically binds to and has a light chain polypeptide comprising an amino acid sequence comprising a CDR
comprising one of the sequences shown in Table 5. In certain embodiments the antibody is a fully human monoclonal antibody.
[0019] A further embodiment is an antibody that binds to TIM-1 and comprises a heavy chain polypeptide comprising an amino acid sequence comprising one of the CDR
sequences shown in Table 4 and a light chain polypeptide comprising an amino acid sequence comprising one of the CDR sequences shown in Table 5. In certain embodiments the antibody is a fully human monoclonal antibody.
[0020] Another embodiment of the invention is a fully human antibody that binds to orthologs of TIM-1. A further embodiment herein is an antibody that cross-competes for binding to TIM-1 with the fully human antibodies described herein.
[0021] Other embodiments includes methods of producing high affinity antibodies to TIM-1 by immunizing a mammal with human TIM-1, or a fragment thereof, and one or more orthologous sequences or fragments thereof.
[0022] It will be appreciated that embodiments of the invention are not limited to any particular form of an antibody. For example, the anti-TIM-1 antibody can be a full length antibody (e.g., having an intact human Fc region) or an antibody fragment (e.g., a Fab, Fab', F(ab')2, Fv, or single chain antibodies). In addition, the antibody can be manufactured from a hybridoma that secretes the antibody, or from a recombinantly produced cell that has been transformed or transfected with a gene or genes encoding the antibody.
[0023] Some embodiments of the invention include isolated nucleic acid molecules encoding any of the anti-TIM-1 antibodies described herein, vectors having an isolated nucleic acid molecule encoding the anti-TIM-1 antibody, and a host cell transformed with such a nucleic acid molecule. In addition, one embodiment of the invention is a method of producing an anti-TIM-1 antibody by culturing host cells under conditions wherein a nucleic acid molecule is expressed to produce the antibody followed by recovering the antibody from the host cell.
[0024] In other embodiments the invention provides compositions, including an antibody, or functional fragment thereof, and a pharmaceutically acceptable carrier.
[0025] In some embodiments, the invention includes pharmaceutical compositions having an effective amount of an anti-TIM-1 antibody in admixture with a pharmaceutically acceptable carrier or diluent. In yet other embodiments, the anti-TIM-1 antibody, or a fragment thereof, is conjugated to a therapeutic agent. The therapeutic agent can be, for example, a toxin, a radioisotope, or a chemotherapeutic agent.
Preferably, such antibodies can be used for the treatment of pathologies, including for example, tumors and cancers, such as ovarian, stomach, endometrial, salivary gland, lung, kidney, colon, colorectal, thyroid, pancreatic, prostate and bladder cancer, as well as other inflammatory conditions. More preferably, the antibodies can be used to treat renal and ovarian carcinomas.
[0026] In still further embodiments, the antibodies described herein can be used for the preparation of a medicament for the effective treatment of TIM-1 induced cell proliferation in an animal, wherein said monoclonal antibody specifically binds to TIM-1.
[0027] Yet another embodiment is the use of an anti-TIM-I antibody in the preparation of a medicament for the treatment of diseases such as neoplasms and inflammatory conditions. In one embodiment, the neoplasm includes, without limitation, tumors and cancers, such as ovarian, stomach, endometrial, salivary gland, lung, kidney, colon, colorectal, thyroid, pancreatic, prostate and bladder cancer.
[0028] In yet another aspect, the invention includes a method for effectively treating pathologies associated with the expression of TIM-1. These methods include selecting an animal in need of treatment for a condition associated with the expression of TIM-1, and administering to said animal a therapeutically effective dose of a fully human monoclonal antibody, wherein said antibody specifically binds to TIM-1.
[0029] Preferably a mammal and, more preferably, a human, receives the anti-TIM-1 antibody. In a preferred embodiment, neoplasms are treated, including, without limitation, renal and pancreatic tumors, head and neck cancer, ovarian cancer, gastric (stomach) cancer, melanoma, lymphoma, prostate cancer, liver cancer, lung cancer, renal cancer, bladder cancer, colon cancer, esophageal cancer, and brain cancer.
[0030] Further embodiments of the invention include the use of an antibody of in the preparation of medicament for the effective treatment of neoplastic disease in an animal, wherein said monoclonal antibody specifically binds to TIM-1. Treatable neoplastic diseases include, for example, ovarian cancer, bladder cancer, lung cancer, glioblastoma, stomach cancer, endometrial cancer, kidney cancer, colon cancer, pancreatic cancer, and prostrate cancer.
[0031] In some embodiments, the invention includes a method for inhibiting cell proliferation associated with the expression of TIM-1. These methods include selecting an animal in need of treatment for TIM-1 induced cell proliferation and administering to said animal a therapeutically effective dose of a fully human monoclonal antibody, wherein the antibody specifically binds TIM-1. In other embodiments, cells expressing TIM-1 are treated with an effective amount of an anti-TIM-1 antibody or a fragment thereof. The method can be performed in vivo.
[0032] The methods can be performed in vivo and the patient is preferably a human patient. In a preferred embodiment, the methods concern the treatment of neoplastic diseases, for example, tumors and cancers, such as renal (kidney) cancer, pancreatic cancer, head and neck cancer, ovarian cancer, gastric (stomach) cancer, melanoma, lymphoma, prostate cancer, liver cancer, breast cancer, lung cancer, bladder cancer, colon cancer, esophageal cancer, and brain cancer.
[0033] In some embodiments, the anti-TIM-1 antibody is administered to a patient, followed by administration of a clearing agent to remove excess circulating antibody from the blood.
[0034] In some embodiments, anti-TIM-I antibodies can be modified to enhance their capability of fixing complement and participating = in complement-dependent cytotoxicity (CDC). In one embodiment, anti-TIM-1 antibodies can be modified, such as by an amino acid substitution, to alter their clearance from the body.
Alternatively, some other amino acid substitutions can slow clearance of the antibody from the body.
-7-.

[0035] In another embodiment, the invention provides an article of manufacture including a container. The container includes a composition containing an anti-antibody, and a package insert or label indicating that the composition can be used to treat neoplastic or inflammatory diseases characterized by the overexpression of TIM-1.
[0036] Yet another embodiment provides methods for assaying the level of TIM-I
in a patient sample, comprising contacting an anti-TIM-1 antibody with a biological sample from a patient, and detecting the level of binding between said antibody and TIM-1 in said sample. In more specific embodiments, the biological sample is blood.
[0037] In one embodiment, the invention includes an assay kit for detecting TIM-1 and TIM-1 orthologs in mammalian tissues or cells to screen for neoplastic diseases or inflammatory conditions. The kit includes an antibody that binds to TIM-1 and a means for indicating the reaction of the antibody with TIM-1, if present. Preferably the antibody is a monoclonal antibody. In one embodiment, the antibody that binds TIM-1 is labeled. In another embodiment the antibody is an unlabeled first antibody and the kit further includes a means for detecting the first antibody. In one embodiment, the means includes a labeled second antibody that is an anti-immunoglobulin. Preferably the antibody is labeled with a marker selected from the group consisting of a fluorochrome, an enzyme, a radionuclide and a radiopaque material.
[0038] Another embodiment of the invention includes a method of diagnosing diseases or conditions in which an antibody prepared as described herein is utilized to detect the level of TIM-1 in a patient sample. In one embodiment, the patient sample is blood or blood serum. In further embodiments, methods for the identification of risk factors, diagnosis of disease, and staging of disease is presented which involves the identification of the overexpression of TIM-1 using anti-TIM-1 antibodies.
[0039] Embodiments of the invention described herein also pertain) to variants of a TIM-1 protein that function as either TIM-1 agonists (mimetics) or as TIM-1 antagonists.
[0040] Another embodiment of the invention is the use of monoclonal antibodies directed against the TIM-1 antigen coupled to cytotoxic chemotherapic agents or radiotherapic agents such as anti-tumor therapeutics.
-8-One embodiment provides an isolated antibody that blocks simultaneous binding to TIM-1 antigen by an antibody having a heavy chain sequence comprising an the amino acid sequence selected from the group consisting of SEQ ID NOS: 2, 6, 10, 14, 18, 22, 26, 30, 34, 38, 42, 46, and 50. Another embodiment provides an isolated antibody that binds to TIM-1 antigen and that cross reacts with an antibody having a heavy chain sequence comprising the amino acid sequence from the group consisting of SEQ ID NOS: 2, 6, 10, 14, 18, 22, 26, 30, 34, 38, 42, 46, and 50.
Another embodiment of the invention provides an isolated antibody that binds to an epitope of SEQ ID NO: 87 on the TIM-1 antigen of SEQ ID NO. 54, and that cross reacts with an antibody having a heavy chain sequence comprising the amino acid sequence selected from the group consisting of SEQ ID NOS: 2, 6, 10, 14, 18, 22, 26, 30, 34, 38, 42, 46, and 50.
In still another embodiment, the invention provides an isolated antibody that binds to an epitope of SEQ ID NO: 87 on the TIM-1 antigen of SEQ ID NO. 54, wherein said antibody blocks simultaneous binding to TIM-1 antigen by an antibody having a heavy chain sequence comprising the amino acid sequence selected from the group comprising SEQ ID
NOS: 2, 6, 10, 14, 18, 22, 26, 30, 34, 38, 42, 46, and 50.
Brief Description of the Drawings [0041] Figure 1 is a bar graph of the results of an ELISA assay of anti-monoclonal antibodies 1.29, 2.56.2, 2.59.2, and 2.45.1 against the TIM-1 antigen.
[0042] Figure 2 is a bar graph of the results of an ELISA assay of anti-monoclonal antibodies 1.29, 2.56.2, 2.59.2, and 2.45.1 against irrelevant protein.
[0043] Figure 3 shows staining of Renal Cell Cancer (3A) and Pancreatic Cancer (3B) with the anti-TIM-1 mAb 2.59.2.
[0044] Figure 4 is a bar graph of clonogenic assay results of anti-TIM-monoclonal antibody mediated toxin killing in the ACHN kidney cancer cell line.
[0045] Figure 5 is a bar graph of clonogenic assay results of anti-TIM-monoclonal antibody mediated toxin killing in the BT549 breast cancer cell line.
-9-[00461 Figure 6 is a bar graph of the results of a clonogenic assay of CAKI-1 cells treated with Auristatin E (AE) conjugated antibodies.
[0047] Figure 7 is a bar graph of the results of a clonogenic assay of BT549 cells treated with Auristatin E (AE) conjugated antibodies.
[00431 Figure 8 is a bar graph showing that anti-TIM-1 monoclonal antibodies 2.59.2, 2.56.2 and 2.45.1 significantly inhibit IL-4 release from 'Thl cells compared to the control PK16.3 mAb.
[0049] Figure 9 is a bar graph showing that anti-TIM-1 monoclonal antibodies 2.59.2 and 2.45.1 significantly inhibit IL-4 release from Th2 cells compared to control PK16.3 mAb.
[0050] Figure 10 is a bar graph showing that anti-TIM-1 monoclonal antibody 2.59.2 significantly inhibited IL-5 release from Thl cells compared to control PK16.3 mAb.
[0051] Figure 11 is a bar graph showing that anti-TIM-1 monoclonal antibodies 2.59.2 and 1.29 significantly inhibited IL-5 release from Th2 cells compared to control P1(16.3 mAb.
[0052] Figure 12 is a bar graph showing that anti-TIM-1 monoclonal antibodies 2.59.2, 1.29 and 2.56.2 significantly inhibited IL-10 release from Thl cells compared to control P1(16.3 mAb.
[00531 Figure 13 is a bar graph showing that anti-TIM-1 monoclonal antibodies 2.59.2, 1.29 and 2.45.1 significantly inhibited IL-10 release from Th2 cells compared to control PK16.3 mAb.
[0054] Figure 14 is a bar graph showing that anti-TIM-1 monoclonal antibodies 2.59.2, 1.29 and 2.56.2 significantly inhibited IL-13 release from Thl cells compared to control PK16.3 mAb.
[00551 Figure 15 is a bar graph showing that anti-TIM-1 monoclonal antibodies 2.59.2 and 1.29 significantly inhibited IL-13 release from Th2 cells compared to control PK16.3 mAb.
[00561 Figure 16 is a bar graph showing that anti-TIM-1 monoclonal antibodies did not inhibit IFNy release from Thl cells compared to control PK16.3 mAb.
-10-[0057] Figure 17 is a bar graph showing that anti-TIM-1 monoclonal antibodies 2.59.2 and 2.45.1 significantly inhibited IFN7 release from Th2 cells compared to control PK16.3 mAb.
[0058] Figures 18A-18T are bar graphs showing BrdU incorporation assay results from experiments in which the neutralization of various human anti-TIM-1 monoclonal antibodies was assessed.
100591 Figures 19A through 19D are line graphs showing the results of antibody conjugate studies performed using the plant toxin Saporin conjugated to TIM-1-specific antibodies and irrelevant antibodies (Figures 19A-19C). Additional negative controls included irrelevant antibodies alone without toxin (Figure 19D).
Detailed Description of the Preferred Embodiment [0060] Embodiments of the invention described herein are based upon the generation and identification of isolated antibodies that bind specifically to T cell, immunoglobulin domain and mucin domain 1 (TIM-1). As discussed below, TIM-1 is expressed at elevated levels in clear cell carcinomas and cancer cell lines derived from the same. Accordingly, antibodies that bind to TIM-1 are useful for the treatment and inhibition of carcinomas. In addition, antibodies that bind TIM-1 are also useful for reducing cell migration and enhancing apoptosis of kidney cancer cells.
[0061] Accordingly, embodiments of the invention described herein provide isolated antibodies, or fragments of those antibodies, that bind to TIM-1. As known in the art, the antibodies can advantageously be, e.g., monoclonal, chimeric and/or human antibodies. Embodiments of the invention described herein also provide cells for producing these antibodies.
[0062] Another embodiment of the invention provides for using these antibodies for diagnostic or therapeutic purposes. For example, embodiments of the invention provide methods and antibodies for inhibiting the expression of TIM-1 associated with cell proliferation. Preferably, the antibodies are used to treat neoplasms such as renal and pancreatic tumors, head and neck cancer, ovarian cancer, gastric (stomach) cancer,
-11-melanoma, lymphoma, prostate cancer, liver cancer, breast cancer, lung cancer, renal cancer, bladder cancer, colon cancer, esophageal cancer, and brain cancer. In association with such treatment, articles of manufacture comprising these antibodies are provided.
Additionally, an , assay kit comprising these antibodies is provided to screen for cancers or tumors.
[0063] Additionally, the nucleic acids described herein, and fragments and variants thereof, may be used, by way of nonlimiting example, (a) to direct the biosynthesis of the corresponding encoded proteins, polypeptides, fragments and variants as recombinant or heterologous gene products, (b) as probes for detection and quantification of the nucleic acids disclosed herein, (c) as sequence templates for preparing antisense molecules, and the like. Such uses are described more fully in the following disclosure.
[0064] Furthermore, the TIM-1 proteins and polypeptides described herein, and fragments and variants thereof, may be used, in ways that include (a) serving as an immunogen to stimulate the production of an anti-TIM-1 antibody, (b) a capture antigen in an immunogenic assay for such an antibody, (c) as a target for screening for substances that bind to a TIM-1 polypeptide described herein, and (d) a target for a TIM-1 specific antibody such that treatment with the antibody affects the molecular and/or cellular function mediated by the target. TIM-1 polypeptide expression or activity can promote cell survival and/or metastatic potential. Conversely, a decrease in TIM-1 polypeptide expression or inhibition of its function reduces tumor cell survival and invasiveness in a therapeutically beneficial manner.
[0065] Single chain antibodies (scFv's) and bispecific antibodies specific for TIM-1 are useful particularly because it may more readily penetrate a tumor mass due to its smaller size relative to a whole IgG molecule. Studies comparing the tumor penetration between whole IgG molecules and scFv's have been have been described in the literature.
The scFv can be derivatized with a toxin or radionuclide in order to destroy tumor cells expressing the TIM-1 antigen, in a manner similar to the IgG2 or IgG4 anti-TIM-1 toxin labeled or radionuclide derivatized whole antibodies already discussed, but with the advantage of being able to penetrate the tumor more fully, which may translate into increased efficacy in eradicating the tumor. A specific example of a biologically active anti-TIM-1 scFv is provided herein.
-12-Sequence Listing [0066] The heavy chain and light chain variable region nucleotide and amino acid sequences of representative human anti-TIM-1 antibodies are provided in the sequence listing, the contents of which are summarized in Table 1 below.
Table 1 mAb SEQ

Sequence ID No.: NO:
Nucleotide sequence encoding the variable region and a portion of the 1 constant region of the heavy chain Amino acid sequence of the variable region of the heavy chain 2 1.29 Nucleotide sequence encoding the variable region and a portion of the 3 constant region of the light chain Amino acid sequence of the variable region of the light chain 4 Nucleotide sequence encoding the variable region and a portion of the 5 constant region of the heavy chain Amino acid sequence of the variable region of the heavy chain 6 1.37 Nucleotide sequence encoding the variable region and a portion of the 7 constant region of the light chain Amino acid sequence of the variable region of the light chain 8 Nucleotide sequence encoding the variable region and a portion of the 9 constant region of the heavy chain Amino acid sequence of the variable region of the heavy chain 10 2.16 Nucleotide sequence encoding the variable region and a portion of the 11 constant region of the light chain Amino acid sequence of the variable region of the light chain 12
-13-Nucleotide sequence encoding the variable region and a portion of the 13 constant region of the heavy chain Amino acid sequence of the variable region of the heavy chain 14 2.17 Nucleotide sequence encoding the variable region and a portion of the 15 constant region of the light chain Amino acid sequence of the variable region of the light chain 16 Nucleotide sequence encoding the variable region and a portion of the 17 constant region of the heavy chain Amino acid sequence of the variable region of the heavy chain 18 2.24 Nucleotide sequence encoding the variable region and a portion of the 19 constant region of the light chain Amino acid sequence of the variable region of the light chain 20 Nucleotide sequence encoding the variable region and a portion of the 21 constant region of the heavy chain Amino acid sequence of the variable region of the heavy chain 22 2.45 Nucleotide sequence encoding the variable region and a portion of the 23 constant region of the light chain Amino acid sequence of the variable region of the light chain 24 Nucleotide sequence encoding the variable region and a portion of the 25 constant region of the heavy chain Amino acid sequence of the variable region of the heavy chain 26 2.54 Nucleotide sequence encoding the variable region and a portion of the ' 27 constant region of the light chain Amino acid sequence of the variable region of the light chain 28 Nucleotide sequence encoding the variable region and a portion of the 29 constant region of the heavy chain Amino acid sequence of the variable region of the heavy chain 30 2.56 Nucleotide sequence encoding the variable region and a portion of the 31 constant region of the light chain Amino acid sequence of the variable region of the light chain 32
-14-Nucleotide sequence encoding the variable region and a portion of the 33 constant region of the heavy chain Amino acid sequence of the variable region of the heavy chain 34 2.59 Nucleotide sequence encoding the variable region and a portion of the 35 constant region of the light chain Amino acid sequence of the variable region of the light chain 36 Nucleotide sequence encoding the variable region and a portion of the 37 constant region of the heavy chain Amino acid sequence of the variable region of the heavy chain 38 2.61 Nucleotide sequence encoding the variable region and a portion of the 39 constant region of the light chain Amino acid sequence of the variable region of the light chain 40 Nucleotide sequence encoding the variable region and a portion of the 41 constant region of the heavy chain 2 70 Amino acid sequence of the variable region of the heavy chain 42 .
Nucleotide sequence encoding the variable region and a portion of the 43 constant region of the light chain Amino acid sequence of the variable region of the light chain 44 Nucleotide sequence encoding the variable region and a portion of the 45 constant region of the heavy chain Amino acid sequence of the variable region of the heavy chain 46 2.76 Nucleotide sequence encoding the variable region and a portion of the 47 constant region of the light chain Amino acid sequence of the variable region of the light chain 48 Nucleotide sequence encoding the variable region and a portion of the 49 ' constant region of the heavy chain Amino acid sequence of the variable region and a porition of the 50 2.70.2 constant region of the heavy chain Nucleotide sequence encoding the variable region and a portion of the 51 constant region of the light chain Amino acid sequence of the variable region and a porition of the 52 constant region of the light chain
-15-Definitions [0067] Unless otherwise defined, scientific and technical terms used in connection with the invention described herein shall have the meanings that are commonly understood by those of ordinary skill in the art. Further, unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular.
Generally, nomenclatures utilized in connection with, and techniques of, cell and tissue culture, molecular biology, and protein and oligo- or polynucleotide chemistry and hybridization described herein are those well known and commonly used in the art. Standard techniques are used for recombinant DNA, oligonucleotide synthesis, and tissue culture and transformation (e.g., electroporation, lipofection). Enzymatic reactions and purification techniques are performed according to manufacturer's specifications or as commonly accomplished in the art or as described herein. The foregoing techniques and procedures are generally performed according to conventional methods well known in the art and as described in various general and more specific references that are cited and discussed throughout the present specification. See e.g., Sambrook et al. Molecular Cloning: A
Laboratory Manual (2d ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
(1989)). The nomenclatures utilized in connection with, and the laboratory procedures and techniques of, analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described herein are those well known and commonly used in the art. Standard techniques are used for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients.
[0068] As utilized in accordance with the present disclosure, the following terms, unless otherwise indicated, shall be understood to have the following meanings:
[0069] The term "TIM-1"
refers to T cell, immunoglobulin domain and mucin domain 1. In one embodiment, TIM-1 refers to a polypeptide comprinsing the amino acid sequence of SEQ ED NO: 54.
[0070] The term "polypeptide" is used herein as a generic term to refer to native protein, fragments, or analogs of a polypeptide sequence. Hence, native protein, fragments, and analogs are species of the polypeptide genus. Preferred polypeptides in accordance with
-16-the invention comprise human heavy chain immunoglobulin molecules and human kappa light chain immunoglobulin molecules, as well as antibody molecules formed by combinations comprising the heavy chain immunoglobulin molecules with light chain immunoglobulin molecules, such as the kappa light chain immunoglobulin molecules, and vice versa, as well as fragments and analogs thereof.
[0071] The term "polynucleotide" as referred to herein means a polymeric form of nucleotides of at least 10 bases in length, either ribonucleotides or deox3mucleotides or a modified form of either type of nucleotide. The term includes single and double stranded forms of DNA.
[0072] The term "isolated polynucleotide" as used herein shall mean a polynucleotide of genomic, cDNA, or synthetic origin or some combination thereof, which by virtue of its origin the isolated polynucleotide (1) is not associated with all or a portion of a polynucleotide in which the isolated polynucleotide is found in nature, (2) is operably linked to a polynucleotide which it is not linked to in nature, or (3) does not occur in nature as part of a larger sequence.
[0073] The term "isolated protein" referred to herein means a protein of cDNA, recombinant RNA, or synthetic origin or some combination thereof, which by virtue of its origin, or source of derivation, the "isolated protein" (1) is not associated with proteins found in nature, (2) is free of other proteins from the same source, e.g., free of murine proteins, (3) is expressed by a cell from a different species, or (4) does not occur in nature.
[0074] The term "oligonucleotide" referred to herein includes naturally occurring, and modified nucleotides linked together by naturally occurring, and non-naturally occurring oligonucleotide linkages. Oligonucleotides are a polynucleotide subset generally comprising a length of 200 bases or fewer. Preferably oligonucleotides are 10 to 60 bases in length and most preferably 12, 13, 14, 15, 16, 17, 18, 19, or 20 to 40 bases in length.
Oligonucleotides are usually single stranded, e.g. for probes; although oligonucleotides may be double stranded, e.g. for use in the construction of a gene mutant. Oligonucleotides described herein can be either sense or antisense oligonucleotides.
[0075] Similarly, unless specified otherwise, the lefthand end of single-stranded polynucleotide sequences is the 5' end; the lefthand direction of double-stranded
-17-polynucleotide sequences is referred to as the 5' direction. The direction of 5' to 3' addition of nascent RNA transcripts is referred to as the transcription direction;
sequence regions on the DNA strand having the same sequence as the RNA and which are 5' to the 5' end of the RNA transcript are referred to as upstream sequences; sequence regions on the DNA strand having the same sequence as the RNA and which are 3' to the 3' end of the RNA
transcript are referred to as downstream sequences.
100761 The term "naturally-occurring" as used herein as applied to an object refers to the fact that an object can be found in nature. For example, a polypeptide or polynucleotide sequence that is present in an organism (including viruses) that can be isolated from a source in nature and which has not been intentionally modified by man in the laboratory or otherwise is naturally-occurring.
[0077] The term "naturally occurring nucleotides" referred to herein includes deoxyribonucleotides and ribonucleotides. The term "modified nucleotides"
referred to herein includes nucleotides with modified or substituted sugar groups and the like. The term "oligonucleotide linkages" referred to herein includes oligonucleotides linkages such as phosphorothioate, phosphorodithioate, phosphoroselenoate, phosphorodiselenoate, phosphoroanilothioate, phoshoraniladate, phosphoroamidate, and the like.
See, e.g., LaPlanche et al., NucL Acids Res. 14:9081 (1986); Stec et al., J. Am. Chem.
Soc. 106:6077 (1984); Stein et al., Nucl. Acids Res. 16:3209 (1988); Zon et aL, Anti-Cancer Drug Design 6:539 (1991); Zon et al., Oligonucleotides and Analogues: A Practical Approach, pp. 87-108 (F. Eckstein, ed., Oxford University Press, Oxford England (1991)); Stec et al., U.S.
Patent No. 5,151,510; Uhlmann and Peyman, Chemical Reviews 90:543 (1990) .
An oligonucleotide can include a label for detection, if desired.
[00781 The term "operably linked" as used herein refers to positions of components so described are in a relationship permitting them to function in their intended manner. A control sequence operably linked to a coding sequence is ligated in such a way that expression of the coding sequence is achieved under conditions compatible with the control sequences.
-18-[0079] The term "control sequence" as used herein refers to polynucleotide sequences which are necessary to effect the expression and processing of coding sequences to which they are ligated. The nature of such control sequences differs depending upon the host organism; in prokaryotes, such control sequences generally include promoter, ribosomal binding site, and transcription termination sequence; in eukaryotes, generally, such control sequences include promoters and transcription termination sequence. The term control sequences is intended to include, at a minimum, all components whose presence is essential for expression and processing, and can also include additional components whose presence is advantageous, for example, leader sequences and fusion pal tiler sequences.
[0080] The term "selectively hybridize" referred to herein means to detectably and specifically bind. Polynucleotides, oligonucleotides and fragments thereof described herein selectively hybridize to nucleic acid strands under hybridization and wash conditions that minimize appreciable amounts of detectable binding to nonspecific nucleic acids. High stringency conditions can be used to achieve selective hybridization conditions as known in the art and discussed herein. Generally, the nucleic acid sequence homology between the polynucleotides, oligonucleotides, and fragments described herein and a nucleic acid sequence of interest will be at least 80%, and more typically with preferably increasing homologies of at least 85%, 90%, 95%, 99%, and 100%.
[0081] Two amino acid sequences are homologous if there is a partial or complete identity between their sequences. For example, 85% homology means that 85% of the amino acids are identical when the two sequences are aligned for maximum matching.
Gaps (in either of the two sequences being matched) are allowed in maximizing matching;
gap lengths of 5 or less are preferred with 2 or less being more preferred. Alternatively and preferably, two protein sequences (or polypeptide sequences derived from them of at least 30 amino acids in length) are homologous, as this term is used herein, if they have an alignment score of at more than 5 (in standard deviation units) using the program ALIGN with the mutation data matrix and a gap penalty of 6 or greater. See Dayhoff, M.O., in Atlas of Protein Sequence and Structure, pp. 101410 (Volume 5, National Biomedical Research Foundation (1972)) and Supplement 2 to this volume, pp. 1-10. The two sequences or parts thereof are
-19-.

more preferably homologous if their amino acids are greater than or equal to 50% identical when optimally aligned using the ALIGN program.
[00821 The term "corresponds to" is used herein to mean that a polynucleotide sequence is homologous (i.e., is identical, not strictly evolutionarily related) to all or a portion of a reference polynucleotide sequence, or that a polypeptide sequence is identical to a reference polypeptide sequence.
[0033] In contradistinction, the term "complementary to" is used herein to mean that the complementary sequence is homologous to all or a portion of a reference polynucleotide sequence. For illustration, the nucleotide sequence "TATAC"
corresponds to a reference sequence "TATAC" and is complementary to a reference sequence "GTATA."
[0084] The following terms are used to describe the sequence relationships between two or more polynucleotide or amino acid sequences: "reference sequence,"
"comparison window," "sequence identity," "percentage of sequence identity,"
and "substantial identity." A "reference sequence" is a defined sequence used as a basis for a sequence comparison; a reference sequence may be a subset of a larger sequence, for example, as a segment of a full-length cDNA or gene sequence given in a sequence listing or may comprise a complete cDNA or gene sequence. Generally, a reference sequence is at least 18 nucleotides or 6 amino acids in length, frequently at least 24 nucleotides or 8 amino acids in length, and often at least 48 nucleotides or 16 amino acids in length. Since two polynucleotides or amino acid sequences may each (1) comprise a sequence (i.e., a portion of the complete polynucleotide or amino acid sequence) that is similar between the two molecules, and (2) may further comprise a sequence that is divergent between the two polynucleotides or amino acid sequences, sequence comparisons between two (or more) molecules are typically performed by comparing sequences of the two molecules over a comparison window to identify and compare local regions of sequence similarity. A
"comparison window," as used herein, refers to a conceptual segment of at least 18 contiguous nucleotide positions or 6 amino acids wherein a polynucleotide sequence or amino acid sequence may be compared to a reference sequence of at least 18 contiguous nucleotides or 6 amino acid sequences and wherein the portion of the polynucleotide sequence in the comparison window may comprise additions, deletions, substitutions, and the
-20-like (i.e., gaps) of 20 percent or less as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences.
Optimal alignment of sequences for aligning a comparison window may be conducted by the local homology algorithm of Smith and Waterman, Adv. Appl. Math., 2:482 (1981), by the homology alignment algorithm of Needleman and Wunsch, J. Mol. Biol., 48:443 (1970), by the search for similarity method of Pearson and Lipman, Proc. Natl. Acad. Sci.
(U.S.A.), 85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package Release 7.0, (Genetics Computer Group, 575 Science Dr., Madison, Wis.), Geneworks, or MacVector software packages), or by inspection, and the best alignment (i.e., resulting in the highest percentage of homology over the comparison window) generated by the various methods is selected.
[0085] The term "sequence identity" means that two polynucleotide or amino acid sequences are identical (i.e., on a nucleotide-by-nucleotide or residue-by-residue basis) over the comparison window. The term percentage of sequence identity is calculated by comparing two optimally aligned sequences over the window of comparison, determining the number of positions at which the identical nucleic acid base (e.g., A, T, C, G, U, or 1) or residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the comparison window (i.e., the window size), and multiplying the result by 100 to yield the percentage of sequence identity. The terms "substantial identity" as used herein denotes a characteristic of a polynucleotide or amino acid sequence, wherein the polynucleotide or amino acid comprises a sequence that has at least 85 percent sequence identity, preferably at least 90 to 95 percent sequence identity, more usually at least 99 percent sequence identity as compared to a reference sequence over a comparison window of at least 18 nucleotide (6 amino acid) positions, frequently over a window of at least 24-48 nucleotide (8-16 amino acid) positions, wherein the percentage of sequence identity is calculated by comparing the reference sequence to the sequence which may include deletions or additions which total 20 percent or less of the reference sequence over the comparison window. The reference sequence may be a subset of a larger sequence.
-21-[0086] As used herein, the twenty conventional amino acids and their abbreviations follow conventional usage. See Immunology - A Synthesis (2nd Edition, E.S.
Golub and D.R. Gren, Eds., Sinauer Associates, Sunderland, Mass. (1991)), which is incorporated herein by reference. Stereoisomers (e.g., D-amino acids) of the twenty conventional amino acids, unnatural amino acids such as cc-, cc-disubstituted amino acids, N-alkyl amino acids, lactic acid, and other unconventional amino acids may also be suitable components for polypeptides described herein. Examples of unconventional amino acids include: 4-hydroxyproline, y -carboxyglutamate, s-N,N,N-trimethyllysine, s-N-acetyllysine, 0-phosphoserine, N-acetylserine, N-formylmethionine, 3-methylhistidine, 5-hydroxylysine, a-N-methylarginine, and other similar amino acids and imino acids (e.g., 4-hydroxyproline).
In the polypeptide notation used herein, the lefthand direction is the amino terminal direction and the righthand direction is the carboxy-terminal direction, in accordance with standard usage and convention.
[0087] As applied to polypeptides, the term "substantial identity"
means that two peptide sequences, when optimally aligned, such as by the programs GAP or BESTFIT using default gap weights, share at least 80 percent sequence identity, preferably at least 90 percent sequence identity, more preferably at least 95 percent sequence identity, and most preferably at least 99 percent sequence identity. Preferably, residue positions which are not identical differ by conservative amino acid substitutions. Conservative amino acid substitutions refer to the interchangeability of residues having similar side chains. For example, a group of amino acids having aliphatic side chains is glycine, alanine, valine, leucine, and isoleucine; a group of amino acids having aliphatic-hydroxyl side chains is serine and threonine; a group of amino acids having amide-containing side chains is asparagine and glutamine; a group of amino acids having aromatic side chains is phenylalanine, tyrosine, and tryptophan; a group of amino acids having basic side chains is lysine, arginine, and histidine;
and a group of amino acids having sulfur-containing side chains is cysteine and methionine.
Preferred conservative amino acids substitution groups are: valine-leucine-isoleucine, phenylalanine-tyrosine, lysine-arginine, alanine-valine, glutamic-aspartic, and asparagine-glutamine.
[00881 As discussed herein, minor variations in the amino acid sequences of antibodies or immunoglobulin molecules are contemplated as being encompassed by the
-22-=
23 PCT/US2004/008502 invention described herein, providing that the variations in the amino acid sequence maintain at least 75%, more preferably at least 80%, 90%, 95%, and most preferably 99%
sequence identity to the antibodies or immunoglobin molecules described herein. In particular, conservative amino acid replacements are contemplated. Conservative replacements are those that take place within a family of amino acids that are related in their side chains.
Genetically encoded amino acids are generally divided into families: (1) acidic=aspartate, glutamate; (2) basic=lysine, arginine, histidine; (3) non-polar=alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan; and (4) uncharged polar=glycine, asparagine, glutamine, cysteine, serine, threonine, tyrosine. More preferred families are:
serine and threonine are aliphatic-hydroxy family; asparagine and glutamine are an amide-containing family; alanine, valine, leucine and isoleucine are an aliphatic family; and phenylalanine, tryptophan, and tyrosine are an aromatic family. For example, it is reasonable to expect that an isolated replacement of a leucine with an isoleucine or valine, an aspartate with a glutamate, a threonine with a serine, or a similar replacement of an amino acid with a structurally related amino acid will not have a major effect on the binding or properties of the resulting molecule, especially if the replacement does not involve an amino acid within a framework site. Whether an amino acid change results in a functional peptide can readily be determined by assaying the specific activity of the polypeptide derivative.
Assays are described in detail herein. Fragments or analogs of antibodies or immunoglobulin molecules can be readily prepared by those of ordinary skill in the art. Preferred amino-and carboxy-termini of fragments or analogs occur near boundaries of functional domains.
Structural and functional domains can be identified by comparison of the nucleotide and/or amino acid sequence data to public or proprietary sequence databases. Preferably, computerized comparison methods are used to identify sequence motifs or predicted protein conformation domains that occur in other proteins of known structure and/or function.
Methods to identify protein sequences that fold into a known three-dimensional structure are known. Bowie et al., Science, 253:164 (1991). Thus, the foregoing examples demonstrate that those of skill in the art can recognize sequence motifs and structural confolinations that may be used to define structural and functional domains described herein.

[0089] Preferred amino acid substitutions are those which: (1) reduce susceptibility to proteolysis, (2) reduce susceptibility to oxidation, (3) alter binding affinity for forming protein complexes, (4) alter binding affinities, and (4) confer or modify other physicochemical or functional properties of such analogs. Analogs can include various muteins of a sequence other than the naturally-occurring peptide sequence. For example, single or multiple amino acid substitutions (preferably conservative amino acid substitutions) may be made in the naturally-occurring sequence (preferably in the portion of the polypeptide outside the domain(s) forming intermolecular contacts). A conservative amino acid substitution should not substantially change the structural characteristics of the parent sequence (e.g., a replacement amino acid should not tend to break a helix that occurs in the parent sequence, or disrupt other types of secondary structure that characterizes the parent sequence). Examples of art-recognized polypeptide secondary and tertiary structures are described in Proteins, Structures and Molecular Principles (Creighton, Ed., W.
H. Freeman and Company, New York (1984)); Introduction to Protein Structure (C. Branden and J.
Tooze, eds., Garland Publishing, New York, N.Y. (1991)); and Thornton et al., Nature, 354:105 (1991) =
[00901 The term "polypeptide fragment" as used herein refers to a polypeptide that has an amino-terminal and/or carboxy-terminal deletion, but where the remaining amino acid sequence is identical to the corresponding positions in the naturally-occurring sequence deduced, for example, from a full-length cDNA sequence. Fragments typically are at least 5, 6, 8 or 10 amino acids long, preferably at least 14 amino acids long, more preferably at least 20 amino acids long, usually at least 50 amino acids long, and even more preferably at least 70 amino acids long. The term "analog" as used herein refers to polypeptides which are comprised of a segment of at least 25 amino acids that has substantial identity to a portion of a deduced amino acid sequence and which has at least one of the following properties: (1) specific binding to a TIM-1, under suitable binding conditions, (2) ability to block appropriate TIM-1 binding, or (3) ability to inhibit the growth and/or survival of TEVI-1 expressing cells in vitro or in vivo. Typically, polypeptide analogs comprise a conservative amino acid substitution (or addition or deletion) with respect to the naturally occurring
-24-sequence. Analogs typically are at least 20 amino acids long, preferably at least 50 amino acids long or longer, and can often be as long as a full-length naturally-occurring polypeptide.
[00911 Peptide analogs are commonly used in the pharmaceutical industry as non-peptide drugs with properties analogous to those of the template peptide.
These types of non-peptide compounds are termed peptide mimetics or peptidomimetics. Fauchere, J.
Adv. Drug Res., 15:29 (1986); Veber and Freidinger, TINS, p.392 (1985); and Evans et al., J. Med.
Chem., 30:1229 (1987) = Such compounds are often developed with the aid of computerized molecular modeling. Peptide mimetics that are structurally similar to therapeutically useful peptides may be used to produce an equivalent therapeutic or prophylactic effect. Generally, peptidomimetics are structurally similar to a paradigm polypeptide (i.e., a polypeptide that has a biochemical property or pharmacological activity), such as human antibody, but have one or more peptide linkages optionally replaced by a linkage selected from the group consisting of: --CH2NH--, --CH2S--, --CH=CH--(cis and trans), --COCH2--, --CH(OH)CH2--, and ¨CH2S0--, by methods well known in the art. Systematic substitution of one or more amino acids of a consensus sequence with a D-amino acid of the same type (e.g., D-lysine in place of L-lysine) may be used to generate more stable peptides. In addition, constrained peptides comprising a consensus sequence or a substantially identical consensus sequence variation may be generated by methods known in the art (Rizo and Gierasch, Ann. Rev. Biochem., 61:387 (1992), incorporated herein by reference); for example, by adding internal cysteine residues capable of forming intramolecular disulfide bridges which cyclize the peptide.
[0092] "Antibody"
or "antibody peptide(s)" refer to an intact antibody, or a binding fragment thereof that competes with the intact antibody for specific binding. Binding fragments are produced by recombinant DNA techniques, or by enzymatic or chemical cleavage of intact antibodies. Binding fragments include Fab, Fab', F(ab')2, Fv, and single-chain antibodies. An antibody other than a bispecific or bifunctional antibody is understood to have each of its binding sites identical. An antibody substantially inhibits adhesion of a receptor to a counterreceptor when an excess of antibody reduces the quantity of receptor bound to counterreceptor by at least about 20%, 40%, 60% or 80%, and more usually greater than about 85% (as measured in an in vitro competitive binding assay).
-25-[0093] Digestion of antibodies with the enzyme, papain, results in two identical antigen-binding fragments, known also as "Fab" fragments, and a "Fe" fragment, having no antigen-binding activity but having the ability to crystallize. Digestion of antibodies with the enzyme, pepsin, results in the a "F(a13')2" fragment in which the two arms of the antibody molecule remain linked and comprise two-antigen binding sites. The F(ab')2 fragment has the ability to crosslink antigen.
[0094] "Fv" when used herein refers to the minimum fragment of an antibody that retains both antigen-recognition and antigen-binding sites.
[0095] "Fab" when used herein refers to a fragment of an antibody which comprises the constant domain of the light chain and the CH1 domain of the heavy chain.
[0096] The term "epitope" includes any protein determinant capable of specific binding to an immunoglobulin or T-cell receptor. Epitopic determinants usually consist of chemically active surface groupings of molecules such as amino acids or sugar side chains and usually have specific three dimensional structural characteristics, as well as specific charge characteristics. An antibody is said to specifically bind an antigen when the dissociation constant is p.M, preferably 5_ 100 nM and most preferably 10 nM.
[0097] The term "agent" is used herein to denote a chemical compound, a mixture of chemical compounds, a biological macromolecule, or an extract made from biological materials.
[0098] The term "pharmaceutical agent" or "drug" as used herein refers to a chemical compound or composition capable of inducing a desired therapeutic effect when properly administered to a patient. Other chemistry terms herein are used according to conventional usage in the art, as exemplified by The McGraw-Hill Dictionary of Chemical Terms (Parker, S., Ed., McGraw-Hill, San Francisco (1985)) =
[0099] The term "antineoplastic agent" is used herein to refer to agents that have the functional property of inhibiting a development or progression of a neoplasm in a human, particularly a malignant (cancerous) lesion, such as a carcinoma, sarcoma, lymphoma, or leukemia. Inhibition of metastasis is frequently a property of antineoplastic agents.
-26-[0100] As used herein, "substantially pure" means an object species is the predominant species present (i.e., on a molar basis it is more abundant than any other individual species in the composition), and preferably a substantially purified fraction is a composition wherein the object species comprises at least about 50 percent (on a molar basis) of all macromolecular species present. Generally, a substantially pure composition will comprise more than about 80 percent of all macromolecular species present in the composition, more preferably more than about 85%, 90%, 95%, and 99%. Most preferably, the object species is purified to essential homogeneity (contaminant species cannot be detected in the composition by conventional detection methods) wherein the composition consists essentially of a single macromolecular species.
[0101] "Active" or "activity" in regard to a TIM-1 polypeptide refers to a portion of a TIM-1 polypeptide which has a biological or an immunological activity of a native TIM-1 polypeptide. "Biological" when used herein refers to a biological function that results from the activity of the native TIM-I polypeptide. A preferred biological activity includes, for example, regulation of cellular growth.
[0102] "Label" or "labeled" as used herein refers to the addition of a detectable moiety to a polypeptide, for example, a radiolabel, fluorescent label, enzymatic label chemiluminescent labeled or a biotinyl group. Radioisotopes or radionuclides may include 3H, 14C, 15N, 35s, 90-y, "Tc, 1 1 lin, 12511 , 1311, fluorescent labels may include rhodamine, lanthanide phosphors or FITC and enzymatic labels may include horseradish peroxidase, 13-galactosidase, luciferase, alkaline phosphatase.
[0103] "Mammal" when used herein refers to any animal that is considered a mammal. Preferably, the mammal is human.
[0104] "Liposome" when used herein refers to a small vesicle that may be useful for delivery of drugs that may include the TIM-1 polypeptide described herein or antibodies to such a TIM-1 polypeptide to a mammal.
[0105] The term "patient" includes human and veterinary subjects.
-27-Antibody Structure [0106] The basic whole antibody structural unit is known to comprise a tetramer.
Each tetramer is composed of two identical pairs of polypeptide chains, each pair having one "light" (about 25 kDa) and one "heavy" chain (about 50-70 kDa). The amino-terminal portion of each chain includes a variable domain of about 100 to 110 or more amino acids primarily responsible for antigen recognition. The carboxy-terminal portion of each chain defines a constant region primarily responsible for effector function. Human light chains are classified as kappa and lambda light chains. Human heavy chains are classified as mu, delta, gamma, alpha, or epsilon, and define the antibody's isotype as IgM, IgG, IgA, and IgE, respectively. Within light and heavy chains, the variable and constant regions are joined by a "J" region of about 12 or more amino acids, with the heavy chain also including a "D" region of about 10 more amino acids. See generally, Fundamental Immunology Ch. 7 (Paul, W., ed., 2d ed. Raven Press, N.Y. (1989)) . The variable regions of each light/heavy chain pair form the antibody binding site.
[0107] The variable domains all exhibit the same general structure of relatively conserved framework regions (FR) joined by three hyper variable regions, also called complementarity determining regions or CDRs. The CDRs from the heavy and light chains of each pair are aligned by the framework regions, enabling binding to a specific epitope.
From N-terminal to C-terminal, both light and heavy chains comprise the domains FR1, CDRI, FR2, CDR2, FR3, CDR3 and FR4. The assignment of amino acids to each region is in accordance with the definitions of Kabat, Sequences of Proteins of Immunological Interest (National Institutes of Health, Bethesda, Md. (1987 and 1991)), or Chothia &
Lesk, J. Mol.
Biol. 196:901-917 (1987); Chothia et al., Nature 342:878-883 (1989).
[0108] A bispecific or bifunctional antibody is an artificial hybrid antibody having two different heavy/light chain pairs and two different binding sites.
Bispecific antibodies can be produced by a variety of methods including fusion of hybridomas or linking of Fab' fragments. See, e.g., Songsivilai & Lachmann, Clin. Exp. Immunol. 79: 315-321 (1990), Kostelny et al., J. Inununol. 148:1547-1553 (1992). Bispecific antibodies do not exist in the form of fragments having a single binding site (e.g., Fab, Fab', and Fv).
-28-[0109] It will be appreciated that such bifunctional or bispecific antibodies are contemplated and encompassed by the invention. A bispecific single chain antibody with specificity to THVI-1 and to the CD3 antigen on cytotoxic T lymphocytes can be used to direct these T cells to tumor cells expressing TIM-1 and cause apoptosis and eradication of the tumor. Two bispecific scFv constructs for this purpose are described herein.
The scFv components specific for TIM-1 can be derived from anti-TIM-1 antibodies described herein.
In some embodiments, the anti-TIM-1 antibody components disclosed in Tables 4 and 5 can be used to generate a biologically active scFv directed against TIM-1. In a preferred embodiment, the scFv components are derived from mAb 2.70. The anti-CD3 scFv component of the therapeutic bispecific scFv was derived from a sequence deposited in Genbank (accession number CAE85148). Alternative antibodies known to target CD3 or other T cell antigens may similarly be effective in treating malignancies when coupled with anti-TIM-1, whether on a single-chain backbone or a full IgG.
Human Antibodies and Humanization of Antibodies [0110]
Embodiments of the invention described herein contemplate and encompass human antibodies. Human antibodies avoid certain of the problems associated with antibodies that possess murine or rat variable and/or constant regions.
The presence of such murine or rat derived proteins can lead to the rapid clearance of the antibodies or can lead to the generation of an immune response against the antibody by a mammal other than a rodent.
Human Antibodies [0111] The ability to clone and reconstruct megabase-sized human loci in YACs and to introduce them into the mouse germline provides a powerful approach to elucidating the functional components of very large or crudely mapped loci as well as generating useful models of human disease. An important practical application of such a strategy is the "humanization" of the mouse humoral immune system.
Introduction of human immunoglobulin (Ig) loci into mice in which the endogenous Ig genes have been inactivated offers the opportunity to develop human antibodies in the mouse. Fully human antibodies are
-29-expected to minimize the immunogenic and allergic responses intrinsic to mouse or mouse-derivatized Mabs and thus to increase the efficacy and safety of the antibodies administered to humans. The use of fully human antibodies can be expected to provide a substantial advantage in the treatment of chronic and recurring human diseases, such as inflammation, autoimmunity, and cancer, which require repeated antibody administrations.
[0112] One approach toward this goal was to engineer mouse strains deficient in mouse antibody production with large fragments of the human Ig loci in anticipation that such mice would produce a large repertoire of human antibodies in the absence of mouse antibodies. This general strategy was demonstrated in connection with our generation of the first XenoMousee strains as published in 1994. See Green et al., Nature Genetics 7:13-21(1994). The XenoMousee strains were engineered with yeast artificial chromosomes (YACs) containing 245 kb and 190 kb-sized germline configuration fragments of the human heavy chain locus and kappa light chain locus, respectively, which contained core variable and constant region sequences. Id.
The XENOMOUSE strains are available from Abgenix, Inc. (Fremont, CA). Greater than approximately 80% of the human antibody repertoire has been introduced through introduction of megabase sized, germline configuration YAC fragments of the human heavy chain loci and kappa light chain loci, respectively, to produce XenoMouseg mice.
[0113] The production of the XENOMOUSE is further discussed and delineated in U.S. Patent Nos. 6,162,963, 6,150,584, 6,114,598, 6,075,181, and 5,939,598 and Japanese Patent Nos. 3 068 180 B2, 3 068 506 B2, and 3 068 507 B2. See also Mendez et al., Nature Genetics 15:146-156 (1997) and Green and Jakobovits, J.
Exp.
Med. 188:483-495 (1998). See also European Patent No. EP 0 463 151 B 1, grant published June 12, 1996, International Patent Application No., WO 94/02602, published February 3, 1994, International Patent Application No., WO 96/34096, published October 31, 1996, WO 98/24893, published June 11, 1998, WO 00/76310, published December 21, 2000.
[0114] Alternative approaches have utilized a "minilocus" approach, in which an exogenous Ig locus is mimicked through the inclusion of pieces (individual genes) from the Ig locus. Thus, one or more VH genes, one or more DH genes, one or more JH
genes, a
-30-mu constant region, and a second constant region (preferably a gamma constant region) are formed into a construct for insertion into an animal. This approach is described in U.S. Patent No. 5,545,807 to Surani et al. and U.S. Patent Nos. 5,545,806, 5,625,825, 5,625,126, 5,633,425, 5,661,016, 5,770,429, 5,789,650, 5,814,318, 5,877,397, 5,874,299, and 6,255,458 each to Lonberg and Kay, U.S. Patent No. 5,591,669 and 6,023,010 to Krimpenfort and Berns, U.S. Patent Nos. 5,612,205, 5,721,367, and 5,789,215 to Berns et al., and U.S. Patent No. 5,643,763 to Choi and Dunn. See also European Patent No. 0 546 073 B 1, International Patent Application Nos. WO 92/03918, WO 92/22645, WO
92/22647, WO 92/22670, WO 93/12227, WO 94/00569, WO 94/25585, WO 96/14436, WO 97/13852, and WO 98/24884 and U.S. Patent No. 5,981,175. See further Taylor et al., 1992, Chen et al., 1993, Tuaillon et al., 1993, Choi et al., 1993, Lonberg et al., (1994), Taylor etal., (1994), and Tuaillon etal., (1995), Fishwild etal., (1996).
[0115] While chimeric antibodies have a human constant region and a murine variable region, it is expected that certain human anti-chimeric antibody (HACA) responses
-31-will be observed, particularly in chronic or multi-dose utilizations of the antibody. Thus, it would be desirable to provide fully human antibodies against TIM-1 in order to vitiate concerns and/or effects of human anti-mouse antibody (HAMA) or HACA response.
Humanization and Display Technologies 101161 Antibodies with reduced immunogenicity can be generated using humanization and library display techniques. It will be appreciated that antibodies can be humanized or primatized using techniques well known in the art. See e.g., Winter and Harris, Inununol Today 14:43-46 (1993) and Wright et al., Crit, Reviews in Inununol.
12:125-168 (1992). The antibody of interest can be engineered by recombinant DNA
techniques to substitute the CH1, CH2, CH3, hinge domains, and/or the framework domain with the corresponding human sequence (see WO 92/02190 and U.S. Patent Nos. 5,530,101, 5,585,089, 5,693,761, 5,693,792, 5,714,350, and 5,777,085). Also, the use of Ig cDNA for construction of chimeric immunoglobulin genes is known in the art (Liu et al., P.N.A.S.
84:3439 (1987) and J brununo/.139:3521 (1987)). rriRNA is isolated from a hybridoma or other cell producing the antibody and used to produce cDNA. The cDNA of interest can be amplified by the polymerase chain reaction using specific primers (U.S. Pat.
Nos. 4,683,195 and 4,683,202). Alternatively, an expression library is made and screened to isolate the sequence of interest encoding the variable region of the antibody is then fused to human constant region sequences. The sequences of human constant regions genes can be found in Kabat et al., "Sequences of Proteins of Immunological Interest," N.I.H.
publication no. 91-3242 (1991). Human C region genes are readily available from known clones. The choice of isotype will be guided by the desired effector functions, such as complement fixation, or activity in antibody-dependent cellular cytotoxicity. Preferred isotypes are IgGl, Ig02 and IgG4. Either of the human light chain constant regions, kappa or lambda, can be used. The chimeric, humanized antibody is then expressed by conventional methods.
Expression vectors include plasmids, retroviruses, YACs, EBV derived episomes, and the like.
[0117] Antibody fragments, such as Fv, F(ab')2 and Fab can be prepared by cleavage of the intact protein, e.g., by protease or chemical cleavage.
Alternatively, a truncated gene is designed. For example, a chimeric gene encoding a portion of the F(ab')2
-32-fragment would include DNA sequences encoding the CH1 domain and hinge region of the H
chain, followed by a translational stop codon to yield the truncated molecule.
[0118] Consensus sequences of H and L J regions can be used to design oligonucleotides for use as primers to introduce useful restriction sites into the J region for subsequent linkage of V region segments to human C region segments. C region cDNA can be modified by site directed mutagenesis to place a restriction site at the analogous position in the human sequence.
[0119] Expression vectors include plasmids, retroviruses, YACs, EBV
derived episomes, and the like. A convenient vector is one that encodes a functionally complete human CH or CL immunoglobulin sequence, with appropriate restriction sites engineered so that any VH or VL sequence can be easily inserted and expressed. In such vectors, splicing usually occurs between the splice donor site in the inserted J region and the splice acceptor site preceding the human C region, and also at the splice regions that occur within the human CH exons. Polyadenylation and transcription termination occur at native chromosomal sites downstream of the coding regions. The resulting chimeric antibody can be joined to any strong promoter, including retroviral LTRs, e.g., SV-40 early promoter, (Okayama et al., Mol. Cell. Bio. 3:280 (1983)), Rous sarcoma virus LTR (Gorman et al., P.N.A.S.
79:6777 (1982)), and moloney murine leukemia virus LTR (Grosschedl et al., Cell 41:885 (1985)).
Also, as will be appreciated, native Ig promoters and the like can be used.
[0120] Further, human antibodies or antibodies from other species can be generated through display-type technologies, including, without limitation, phage display, retroviral display, ribosomal display, and other techniques, using techniques well known in the art and the resulting molecules can be subjected to additional maturation, such as affinity maturation, as such techniques are well known in the art. Wright and Harris, supra., Hanes and Plucthau, PNAS USA 94:4937-4942 (1997) (ribosomal display), Parmley and Smith, Gene 73:305-318 (1988) (phage display), Scott, TIBS 17:241-245 (1992), Cwirla et al., PNAS
USA 87:6378-6382 (1990), Russel et al., Nucl. Acids Res. 21:1081-1085 (1993), Hoganboom et al., InimUllol. Reviews 130:43-68 (1992), Chi swell and McCafferty, TIBTECH
10:80-84 (1992), and U.S. Patent No. 5,733,743. If display technologies are utilized to produce antibodies that are not human, such antibodies can be humanized as described above.
-33-[0121]
Using these techniques, antibodies can be generated to TIM-1 expressing cells, TIM-1 itself, forms of TIM-1, epitopes or peptides thereof, and expression libraries thereto (see e.g. U.S. Patent No. 5,703,057) which can thereafter be screened as described above for the activities described above.
Antibody Therapeutics [0122]
In certain respects, it can be desirable in connection with the generation of antibodies as therapeutic candidates against TIM-1 that the antibodies be capable of fixing complement and participating in complement-dependent cytotoxicity (CDC).
Such antibodies include, without limitation, the following: murine IgM, murine IgG2a, murine IgG2b, murine IgG3, human IgM, human IgGl, and human IgG3. It will be appreciated that antibodies that are generated need not initially possess such an isotype but, rather, the antibody as generated can possess any isotype and the antibody can be isotype switched thereafter using conventional techniques that are well known in the art. Such techniques , include the use of direct recombinant techniques (see, e.g., U.S.
Patent No. 4,816,397), cell-cell fusion techniques (see, e.g., U.S. Patent Nos. 5,916,771 and 6,207,418), among others.
[0123]
In the cell-cell fusion technique, a myeloma or other cell line is prepared that possesses a heavy chain with any desired isotype and another myeloma or other cell line is prepared that possesses the light chain. Such cells can, thereafter,, be fused and a cell line expressing an intact antibody can be isolated.
[0124]
By way of example, the TIM-1 antibody discussed herein is a human anti-TIM-1 IgG2 antibody. If such antibody possessed desired binding to the TIM-1 molecule, it could be readily isotype switched to generate a human IgM, human IgGl, or human IgG3 isotype, while still possessing the same variable region (which defines the antibody's specificity and some of its affinity). Such molecule would then be capable of fixing complement and participating in CDC.
-34-Design and Generation of Other Therapeutics [0125] Due to their association with renal and pancreatic tumors, head and neck cancer, ovarian cancer, gastric (stomach) cancer, melanoma, lymphoma, prostate cancer, liver cancer, breast cancer, lung cancer, renal cancer, bladder cancer, colon cancer, esophageal cancer, and brain cancer, antineoplastic agents comprising anti-TIM-1 antibodies are contemplated and encompassed by the invention.
[0126] Moreover, based on the activity of the antibodies that are produced and characterized herein with respect to TIM-1, the design of other therapeutic modalities beyond antibody moieties is facilitated. Such modalities include, without limitation, advanced antibody therapeutics, such as bispecific antibodies, immunotoxins, and radiolabeled therapeutics, generation of peptide therapeutics, gene therapies, particularly intrabodies, antisense therapeutics, and small molecules.
[0127] In connection with the generation of advanced antibody therapeutics, where complement fixation is a desirable attribute, it can be possible to sidestep the dependence on complement for cell killing through the use of bispecifics, immunotoxins, or radiolabels, for example.
[0128] For example, in connection with bispecific antibodies, bispecific antibodies can be generated that comprise (i) two antibodies one with a specificity to TIM-1 and another to a second molecule that are conjugated together, (ii) a single antibody that has one chain specific to TIM-1 and a second chain specific to a second molecule, or (iii) a single chain antibody that has specificity to TIM-1 and the other molecule. Such bispecific antibodies can be generated using techniques that are well known for example, in connection with (i) and (ii) see, e.g., Fanger et al., Itninunol Methods 4:72-81 (1994) and Wright and Harris, supra and in connection with (iii) see, e.g., Traunecker et al., Int.
J. Cancer (Suppl) 7:51-52 (1992). In each case, the second specificity can be made to the heavy chain activation receptors, including, without limitation, CD16 or CD64 (see, e.g., Deo et al., 18:127 (1997)) or CD89 (see, e.g., Valerius et al., Blood 90:4485-4492 (1997)). Bispecific antibodies prepared in accordance with the foregoing would be likely to kill cells expressing TIM-1, and particularly those cells in which the TIM-1 antibodies described herein are effective.
-35-[0129] With respect to immunotoxins, antibodies can be modified to act as immunotoxins utilizing techniques that are well known in the art. See, e.g., Vitetta, Immunol Today 14:252 (1993). See also U.S. Patent No. 5,194,594. In connection with the preparation of radiolabeled antibodies, such modified antibodies can also be readily prepared utilizing techniques that are well known in the art. See, e.g., Junghans et al., in Cancer Chemotherapy and Biotherapy 655-686 (2d ed., Chafner and Longo, eds., Lippincott Raven (1996)). See also U.S. Patent Nos. 4,681,581, 4,735,210, 5,101,827, 5,102,990 (RE 35,500), 5,648,471, and 5,697,902. Each of immunotoxins and radiolabeled molecules would be likely to kill cells expressing TIM-1, and particularly those cells in which the antibodies described herein are effective.
[0130] In connection with the generation of therapeutic peptides, through the utilization of structural infolination related to TIM-1 and antibodies thereto, such as the antibodies described herein (as discussed below in connection with small molecules) or screening of peptide libraries, therapeutic peptides can be generated that are directed against TIM-1. Design and screening of peptide therapeutics is discussed in connection with Houghten et al., Bioteclmiques 13:412-421 (1992), Houghten, PNAS USA 82:5131-(1985), Pinalla et al., Biotechniques 13:901-905 (1992), Blake and Litzi-Davis, BioConjugate Chem. 3:510-513 (1992). Immunotoxins and radiolabeled molecules can also be prepared, and in a similar manner, in connection with peptidic moieties as discussed above in connection with antibodies.
[0131]
Assuming that the TIM-1 molecule (or a form, such as a splice variant or alternate forni) is functionally active in a disease process, it will also be possible to design gene and antisense therapeutics thereto through conventional techniques. Such modalities can be utilized for modulating the function of TIM-1. In connection therewith the antibodies, as described herein, facilitate design and use of functional assays related thereto. A design and strategy for antisense therapeutics is discussed in detail in International Patent Application No. WO 94/29444. Design and strategies for gene therapy are well known.
However, in particular, the use of gene therapeutic techniques involving intrabodies could prove to be particularly advantageous. See, e.g., Chen et al., Human Gene Therapy 5:595-601 (1994) and Marasco, Gene Therapy 4:11-15 (1997).
General design of and
-36-considerations related to gene therapeutics is also discussed in International Patent Application No. WO 97/38137.
[0132] Small molecule therapeutics can also be envisioned. Drugs can be designed to modulate the activity of TIM-1, as described herein. Knowledge gleaned from the structure of the TIM-1 molecule and its interactions with other molecules, as described herein, such as the antibodies described herein, and others can be utilized to rationally design additional therapeutic modalities. In this regard, rational drug design techniques such as X-ray crystallography, computer-aided (or assisted) molecular modeling (CAMM), quantitative or qualitative structure-activity relationship (QSAR), and similar technologies can be utilized to focus drug discovery efforts. Rational design allows prediction of protein or synthetic structures which can interact with the molecule or specific forms thereof which can be used to modify or modulate the activity of TIM-1. Such structures can be synthesized chemically or expressed in biological systems. This approach has been reviewed in Capsey et al., Genetically Engineered Human Therapeutic Drugs (Stockton Press, NY (1988)).
Further, combinatorial libraries can be designed and synthesized and used in screening programs, such as high throughput screening efforts.
TIM-1 Agonists And Antagonists [0133] Embodiments of the invention described herein also pertain to variants of a TIM-1 protein that function as either TIM-1 agonists (mimetics) or as TIM-1 antagonists.
Variants of a TIM-1 protein can be generated by mutagenesis, e.g., discrete point mutation or truncation of the TIM-1 protein. An agonist of the TIM-1 protein can retain substantially the same, or a subset of, the biological activities of the naturally occurring form of the TIM-1 protein. An antagonist of the TIM-1 protein can inhibit one or more of the activities of the naturally occurring form of the T11\4-1 protein by, for example, competitively binding to a downstream or upstream member of a cellular signaling cascade which includes the TIM-1 protein. Thus, specific biological effects can be elicited by treatment with a variant of limited function. In one embodiment, treatment of a subject with a variant having a subset of the biological activities of the naturally occurring form of the protein has fewer side effects in a subject relative to treatment with the naturally occurring foun of the TIM-1 protein.
-37-[0134] Variants of the TIM-1 protein that function as either TIM-1 agonists (mimetics) or as TIM-1 antagonists can be identified by screening combinatorial libraries of mutants, e.g., truncation mutants, of the TIM-1 protein for protein agonist or antagonist activity. In one embodiment, a variegated library of TIM-1 variants is generated by combinatorial mutagenesis at the nucleic acid level and is encoded by a variegated gene library. A variegated library of TIM-1 variants can be produced by, for example, enzymatically ligating a mixture of synthetic oligonucleotides into gene sequences such that a degenerate set of potential TIM-1 sequences is expressible as individual polypeptides, or alternatively, as a set of larger fusion proteins (e.g., for phage display) containing the set of TIM-1 sequences therein. There are a variety of methods which can be used to produce libraries of potential TIM-1 variants from a degenerate oligonucleotide sequence. Chemical synthesis of a degenerate gene sequence can be performed in an automatic DNA
synthesizer, and the synthetic gene then ligated into an appropriate expression vector. Use of a degenerate set of genes allows for the provision, in one mixture, of all of the sequences encoding the desired set of potential TIM-1 variant sequences. Methods for synthesizing degenerate oligonucleotides are known in the art (see, e.g., Narang, Tetrahedron 39:3 (1983); Itakura et al., Annu. Rev. Biochem. 53:323 (1984); Itakura et al., Science 198:1056 (1984); Ike et al., Nucl. Acid Res. 11:477 (1983).
Radioimmuno & Immunochemotherapeutic Antibodies [0135] Cytotoxic chemotherapy or radiotherapy of cancer is limited by serious, sometimes life-threatening, side effects that arise from toxicities to sensitive normal cells because the therapies are not selective for malignant cells. Therefore, there is a need to improve the selectivity. One strategy is to couple therapeutics to antibodies that recognize tumor-associated antigens. This increases the exposure of the malignant cells to the ligand-targeted therapeutics but reduces the exposure of normal cells to the same agent. See Allen, Nat. Rev. Cancer 2(10):750-63 (2002).
[0136) The TIM-1 antigen is one of these tumor-associated antigens, as shown by its specific expression on cellular membranes of tumor cells by FACS and IHC.
Therefore one embodiment of the invention is to use monoclonal antibodies directed against the TIM-1
-38-antigen coupled to cytotoxic chemotherapic agents or radiotherapic agents as anti-tumor therapeutics.
[0137] Radiolabels are known in the art and have been used for diagnostic or therapeutic radioimmuno conjugates. Examples of radiolabels includes, but are not limited to, the following: radioisotopes or radionuclides (e.g., 3H, 14C, 15N, 35S, 90Y, 99Tc, 111In, 1251, 1311, 177Lu, Rhenium-186, Rhenium-188, Samarium-153, Copper-64, Scandium-47).
For example, radionuclides which have been used in radioimmunoconjugate guided clinical diagnosis include, but are not limited to: 131 I, 125 I, 123 I, 99 Tc, 67 Ga, as well as 111 In.
Antibodies have also been labeled with a variety of radionuclides for potential use in targeted immunotherapy (see Peirersz et al., 1987). Monoclonal antibody conjugates have also been used for the diagnosis and treatment of cancer (e.g., Immunol. Cell Biol.
65:111-125). These radionuclides include, for example, 188 Re and 186 Re as well as 90 Y, and to a lesser extent 199 Au and 67 Cu. I-(131) have also been used for therapeutic purposes. U.S.
Patent No. 5,460,785 provides a listing of such radioisotopes. Radiotherapeutic chelators and chelator conjugates are known in the art. See U.S. Patent Nos. 4,831,175, 5,099,069, 5,246,692, 5,286,850, and 5,124,471.
[0138] Immunoradiopharmaceuticals utilizing anti-TIM-1 antibodies can be prepared utilizing techniques that are well known in the art. See, e.g., Junghans et al., in Cancer Chemotherapy and Biotherapy 655-686 (2d ed., Chafner and Longo, eds., Lippincott Raven (1996)), U.S. Patent Nos. 4,681,581, 4,735,210, 5,101,827, RE 35,500, 5,648,471, and 5,697,902.
[0139] Cyotoxic immunoconjugates are known in the art and have been used as therapeutic agents. Such immunoconjugates may for example, use maytansinoids (U.S.
Patent No. 6,441,163), tubulin polymerization inhibitor, auristatin (Mohammad et al., Int. .I.
Oncol. 15(2):367-72 (1999); Doronina et al., Nature Biotechnology 21(7):778-784 (2003)), dolastatin derivatives (Ogawa et al., Toxicol Lett. 121(2):97-106 (2001);
21(3)778-784), Mylotarg (Wyeth Laboratories, Philidelphia, PA); maytansinoids (DM1), taxane or mertansine (ImmunoGen Inc.). Immunotoxins utilizing anti-TIM-1 antibodies may be prepared by techniques that are well known in the art. See, e.g., Vitetta, Inununol Today 14:252 (1993); U.S. Patent No. 5,194,594.
-39-[0140] Bispecific antibodies may be generated using techniques that are well known in the art for example, see, e.g., Fanger et al., Immunol Methods 4:72-81 (1994);
Wright and Harris, supra; Traunecker et al., Int. J. Cancer (Suppl.) 7:51-52 (1992). In each case, the first specificity is to TIM-1, the second specificity may be made to the heavy chain activation receptors, including, without limitation, CD16 or CD64 (see, e.g., Deo et al., 18:127 (1997)) or CD89 (see, e.g., Valerius et al., Blood 90:4485-4492 (1997)). Bispecific antibodies prepared in accordance with the foregoing would kill cells expressing TIM-1.
[0141] Depending on the intended use of the antibody, i.e., as a diagnostic or therapeutic reagent, radiolabels are known in the art and have been used for similar purposes.
For example, radionuclides which have been used in clinical diagnosis include, but are not limited to: 131 I, 125 I, 123 I, 99 Tc, 67 Ga, as well as 111 In.
Antibodies have also been labeled with a variety of radionuclides for potential use in targeted immunotherapy. See Peirersz et al., (1987). Monoclonal antibody conjugates have also been used for the diagnosis and treatment of cancer. See, e.g., Immunol. Cell Biol.
65:111-125.
These radionuclides include, for example, 188 Re and 186 Re as well as 90 Y, and to a lesser extent 199 Au and 67 Cu. I-(131) have also been used for therapeutic purposes. U.S. Pat. No. 5,460,785 provides a listing of such radioisotopes.
[0142] Patents relating to radiotherapeutic chelators and chelator conjugates are known in the art. For example, U.S. Pat. No. 4,831,175 of Gansow is directed to polysubstituted diethylenetriaminepentaacetic acid chelates and protein conjugates containing the same, and methods for their preparation. U.S. Pat. Nos. 5,099,069, 5,246,692, 5,286,850, and 5,124,471 of Gansow also relate to polysubstituted DTPA chelates.
[0143] Cytotoxic chemotherapies are known in the art and have been used for similar purposes. For example, U.S. Pat. No. 6,441,163 describes the process for the production of cytotoxic conjugates of maytansinoids and antibodies. The anti-tumor activity of a tubulin polymerization inhibitor, auristatin PE, is also known in the art. Mohammad et al., Int. J. Oncol. 15(2):367-72 (Aug 1999).
-40-Preparation of Antibodies [0144] Briefly, XenoMousee lines of mice were immunized with TIM-1 protein, lymphatic cells (such as B-cells) were recovered from the mice that express antibodies and were fused with a myeloid-type cell line to prepare immortal hybridoma cell lines, and such hybridoma cell lines were screened and selected to identify hybridoma cell lines that produce antibodies specific to TIM-1. Alternatively, instead of being fused to myeloma cells to generate hybridomas, the recovered B cells, isolated from immunized XenoMouse lines of mice, with reactivity against TIM-1 (determined by e.g. ELISA with TIM-1-His protein), were then isolated using a TIM-1-specific hemolytic plaque assay. Babcook et al., Proc.
Natl. Acad. Sci. USA, 93:7843-7848 (1996). In this assay, target cells such as sheep red blood cells (SRBCs) were coated with the TIM-1 antigen. In the presence of a B
cell culture secreting the anti-TIM-1 antibody and complement, the formation of a plaque indicates specific TIM-1-mediated lysis of the target cells. Single antigen-specific plasma cells in the center of the plaques were isolated and the genetic information that encodes the specificity of the antibody isolated from single plasma cells.
[0145] Using reverse-transcriptase PCR, the DNA encoding the variable region of the antibody secreted was cloned and inserted into a suitable expression vector, preferably a vector cassette such as a pcDNA, more preferably the pcDNA vector containing the constant domains of immunglobulin heavy and light chain. The generated vector was then be transfected into host cells, preferably CHO cells, and cultured in conventional nutrient media modified as appropriate for inducing promoters, selecting transformants, or amplifying the genes encoding the desired sequences.
[0146] In general, antibodies produced by the above-mentioned cell lines possessed fully human IgG2 heavy chains with human kappa light chains. The antibodies possessed high affinities, typically possessing Kd's of from about 10-6 through about 10-11 M, when measured by either solid phase and solution phase. These mAbs can be stratified into groups or "bins" based on antigen binding competition studies, as discussed below.
[0147] As will be appreciated, antibodies, as described herein, can be expressed in cell lines other than hybridoma cell lines. Sequences encoding particular antibodies can be used for transformation of a suitable mammalian host cell. Transfoiniation can be by any
-41-known method for introducing polynucleotides into a host cell, including, for example packaging the polynucleotide in a virus (or into a viral vector) and transducing a host cell with the virus (or vector) or by transfection procedures known in the art, as exemplified by U.S. Patent Nos. 4,399,216, 4,912,040, 4,740,461, and 4,959,455.
The transformation procedure used depends upon the host to be transformed. Methods for introduction of heterologous polynucleotides into mammalian cells are well known in the art and include dextran-mediated transfection, calcium phosphate precipitation, polybrene mediated transfection, protoplast fusion, electroporation, encapsulation of the polynucleotide(s) in liposomes, and direct microinjection of the DNA
into nuclei.
[0148] Mammalian cell lines available as hosts for expression are well known in the art and include many immortalized cell lines available from the American Type Culture Collection (ATCC), including but not limited to Chinese hamster ovary (CHO) cells, HeLa cells, baby hamster kidney (BHK) cells, monkey kidney cells (COS), human hepatocellular carcinoma cells (e.g., Hep G2), and a number of other cell lines. Cell lines of particular preference are selected through determining which cell lines have high expression levels and produce antibodies with constitutive TIM-1 binding properties.
Therapeutic Administration and Formulations [0149] The compounds of the invention are formulated according to standard practice, such as prepared in a carrier vehicle. The term "pharmacologically acceptable carrier" means one or more organic or inorganic ingredients, natural or synthetic, with which the mutant proto-oncogene or mutant oncoprotein is combined to facilitate its application. A
suitable carrier includes sterile saline although other aqueous and non-aqueous isotonic sterile solutions and sterile suspensions known to be pharmaceutically acceptable are known to those of ordinary skill in the art. In this regard, the term "carrier"
encompasses liposomes and the antibody (See Chen et al., Anal. Biocheni. 227: 168-175 (1995) as well as any plasmid and viral expression vectors.
[01501 Any of the novel polypeptides of this invention may be used in the form of a pharmaceutically acceptable salt. Suitable acids and bases which are capable of forming
-42-salts with the polypeptides of the present invention are well known to those of skill in the art, and include inorganic and organic acids and bases.
[0151] A compound of the invention is administered to a subject in a therapeutically-effective amount, which means an amount of the compound which produces a medically desirable result or exerts an influence on the particular condition being treated. An effective amount of a compound of the invention is capable of ameliorating or delaying progression of the diseased, degenerative or damaged condition. The effective amount can be determined on an individual basis and will be based, in part, on consideration of the physical attributes of the subject, symptoms to be treated and results sought. An effective amount can be determined by one of ordinary skill in the art employing such factors and using no more than routine experimentation.
[0152] The compounds of the invention may be administered in any manner which is medically acceptable. This may include injections, by parenteral routes such as intravenous, intravascular, intraarterial, subcutaneous, intramuscular, intratumor, intraperitoneal, intraventricular, intraepidural, or others as well as oral, nasal, ophthalmic, rectal, or topical. Sustained release administration is also specifically included in the invention, by such means as depot injections or erodible implants. Localized delivery is particularly contemplated, by such means as delivery via a catheter to one or more arteries, such as the renal artery or a vessel supplying a localized tumor.
[0153] Biologically active anti-TIM-1 antibodies as described herein can be used in a sterile pharmaceutical preparation or formulation to reduce the level of serum TIM-1 thereby effectively treating pathological conditions where, for example, serum TIM-1 is abnormally elevated. Anti-TIM-1 antibodies preferably possess adequate affinity to potently suppress TIM-1 to within the target therapeutic range, and preferably have an adequate duration of action to allow for infrequent dosing. A, prolonged duration of action will allow for less frequent and more convenient dosing schedules by alternate parenteral routes such as subcutaneous or intramuscular injection.
[0154] When used for in vivo administration, the antibody foimulation must be sterile. This is readily accomplished, for example, by filtration through sterile filtration membranes, prior to or following lyophilization and reconstitution. The antibody ordinarily
-43-will be stored in lyophilized form or in solution. Therapeutic antibody compositions generally are placed into a container having a sterile access port, for example, an intravenous solution bag or vial having an adapter that allows retrieval of the formulation, such as a stopper pierceable by a hypodermic injection needle.
[0155] The route of antibody administration is in accord with known methods, e.g., injection or infusion by intravenous, intraperitoneal, intracerebral, intramuscular, intraocular, intraarterial, intrathecal, inhalation or intralesional routes, or by sustained release systems as noted below. The antibody is preferably administered continuously by infusion or by bolus injection.
[0156] An effective amount of antibody to be employed therapeutically will depend, for example, upon the therapeutic objectives, the route of administration, and the condition of the patient. Accordingly, it is preferred that the therapist titer the dosage and modify the route of administration as required to obtain the optimal therapeutic effect.
Typically, the clinician will administer antibody until a dosage is reached that achieves the desired effect. The progress of this therapy is easily monitored by conventional assays or by the assays described herein.
[0157] Antibodies, as described herein, can be prepared in a mixture with a pharmaceutically acceptable carrier. This therapeutic composition can be administered intravenously or through the nose or lung, preferably as a liquid or powder aerosol (lyophilized). The composition can also be administered parenterally or subcutaneously as desired. When administered = systemically, the therapeutic composition should be sterile, pyrogen-free and in a parenterally acceptable solution having due regard for pH, isotonicity, and stability. These conditions are known to those skilled in the art.
Briefly, dosage formulations of the compounds described herein are prepared for storage or administration by mixing the compound having the desired degree of purity with physiologically acceptable carriers, excipients, or stabilizers. Such materials are non-toxic to the recipients at the dosages and concentrations employed, and include buffers such as TRIS HC1, phosphate, citrate, acetate and other organic acid salts; antioxidants such as ascorbic acid; low molecular weight (less than about ten residues) peptides such as polyarginine, proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidinone;
-44-amino acids such as glycine, glutamic acid, aspartic acid, or arginine;
monosaccharides, disaccharides, and other carbohydrates including cellulose or its derivatives, glucose, mannose, or dextrins; chelating agents such as EDTA; sugar alcohols such as mannitol or sorbitol; counterions such as sodium and/or nonionic surfactants such as TWEENTm, PLURONICS or polyethyleneglycol.
[0158] Sterile compositions for injection can be formulated according to conventional pharmaceutical practice as described in Remington: The Science and Practice of Pharmacy (20th ed, Lippincott Williams & Wilkens Publishers (2003)). For example, dissolution or suspension of the active compound in a vehicle such as water or naturally occurring vegetable oil like sesame, peanut, or cottonseed oil or a synthetic fatty vehicle like ethyl oleate or the like can be desired. Buffers, preservatives, antioxidants and the like can be incorporated according to accepted pharmaceutical practice.
[0159] Suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing the polypeptide, which matrices are in the form of shaped articles, films or microcapsules. Examples of sustained-release matrices include polyesters, hydrogels (e.g., poly(2-hydroxyethyl-methacrylate) as described by Langer et al., J. Biomed Mater. Res., (1981) 15:167-277 and Langer, Chem.
Tech., (1982) 12:98-105, or poly(vinylalcohol)), polylactides (U.S. Pat. No.
3,773,919, EP
58,481), copolymers of L-glutamic acid and gamma ethyl-L-glutamate (Sidman et al., Biopolymers, (1983) 22:547-556), non-degradable ethylene-vinyl acetate (Langer et al., supra), degradable lactic acid-glycolic acid copolymers such as the LUPRON
DepotTM
(injectable microspheres composed of lactic acid-glycolic acid copolymer and leuprolide acetate), and poly-D-(-)-3-hydroxybutyric acid (EP 133,988).
[0160] While polymers such as ethylene-vinyl acetate and lactic acid-glycolic acid enable release of molecules for over 100 days, certain hydrogels release proteins for shorter time periods. When encapsulated proteins remain in the body for a long time, they can denature or aggregate as a result of exposure to moisture at 37 C, resulting in a loss of biological activity and possible changes in immunogenicity. Rational strategies can be devised for protein stabilization depending on the mechanism involved. For example, if the aggregation mechanism is discovered to be intermolecular S-S bond formation through
-45-disulfide interchange, stabilization can be achieved by modifying sulfhydryl residues, lyophilizing from acidic solutions, controlling moisture content, using appropriate additives, and developing specific polymer matrix compositions.
[01611 Sustained-released compositions also include preparations of crystals of the antibody suspended in suitable formulations capable of maintaining crystals in suspension. These preparations when injected subcutaneously or intraperitonealy can produce a sustained release effect. Other compositions also include liposomally entrapped antibodies. Liposomes containing such antibodies are prepared by methods known per se:
U.S. Pat. No. DE 3,218,121; Epstein et al., Proc. Natl. Acad. Sci. USA, (1985) 82:3688-3692;
Hwang et al., Proc. Natl. Acad. Sci. USA, (1980) 77:4030-4034; EP 52,322; EP
36,676; EP
88,046; EP 143,949; 142,641; Japanese patent application 83-118008; U.S. Pat.
Nos. 4,485,045 and 4,544,545; and EP 102,324.
[01621 The dosage of the antibody formulation for a given patient will be determined by the attending physician taking into consideration various factors known to modify the action of drugs including severity and type of disease, body weight, sex, diet, time and route of administration, other medications and other relevant clinical factors.
Therapeutically effective dosages can be determined by either in vitro or in vivo methods.
[0163] An effective amount of the antibodies, described herein, to be employed therapeutically will depend, for example, upon the therapeutic objectives, the route of administration, and the condition of the patient. Accordingly, it is preferred for the therapist to titer the dosage and modify the route of administration as required to obtain the optimal therapeutic effect. A typical daily dosage might range from about 0.001mg/kg to up to 100mg/kg or more, depending on the factors mentioned above. Typically, the clinician will administer the therapeutic antibody until a dosage is reached that achieves the desired effect.
The progress of this therapy is easily monitored by conventional assays or as described herein.
[0164] It will be appreciated that administration of therapeutic entities in accordance with the compositions and methods herein will be administered with suitable carriers, excipients, and other agents that are incorporated into formulations to provide improved transfer, delivery, tolerance, and the like. These formulations include, for example,
-46-powders, pastes, ointments, jellies, waxes, oils, lipids, lipid (cationic or anionic) containing vesicles (such as LipofectinTm), DNA conjugates, anhydrous absorption pastes, oil-in-water and water-in-oil emulsions, emulsions carbowax (polyethylene glycols of various molecular weights), semi-solid gels, and semi-solid mixtures containing carbowax. Any of the foregoing mixtures can be appropriate in treatments and therapies in accordance with the present invention, provided that the active ingredient in the formulation is not inactivated by the formulation and the formulation is physiologically compatible and tolerable with the route of administration. See also Baldrick P. "Phandaceutical excipient development: the need for preclinical guidance." Regul. Toxicol. Pharnzacol. 32(2):210-8 (2000), Wang W.
"Lyophilization and development of solid protein pharmaceuticals." Int. J
Pharnz. 203(1-2):1-60 (2000), Charman WN "Lipids, lipophilic drugs, and oral drug delivery-some emerging Concepts." J Pharm Sci .89(8):967-78 (2000), Powell et al.
"Compendium of excipients for parenteral formulations" FDA J Pharm Sci Technol. 52:238-311(1998) and the citations therein for additional information related to formulations, excipients and carriers well known to pharmaceutical chemists.
[0165] It is expected that the antibodies described herein will have therapeutic effect in treatment of symptoms and conditions resulting from TIM-1 expression. In specific embodiments, the antibodies and methods herein relate to the treatment of symptoms resulting from TIM-1 expression including symptoms of cancer. Further embodiments, involve using the antibodies and methods described herein to treat cancers, such as cancer of the lung, colon, stomach, kidney, prostrate, or ovary.
Diagnostic Use [0166] TIM-1 has been found to be expressed at low levels in normal kidney but its expression is increased dramatically in postischemic kidney. Ichimura et al., J. Biol.
Chem. 273(7):4135-42 (1998). As immunohistochemical staining with anti-TIM-1 antibody shows positive staining of renal, kidney, prostate and ovarian carcinomas (see below), TIM-1 overexpression relative to normal tissues can serve as a diagnostic marker of such diseases.
[0167] Antibodies, including antibody fragments, can be used to qualitatively or quantitatively detect the expression of TIM-1 proteins. As noted above, the antibody
-47-preferably is equipped with a detectable, e.g., fluorescent label, and binding can be monitored by light microscopy, flow cytometry, fluorimetry, or other techniques known in the art.
These techniques are particularly suitable if the amplified gene encodes a cell surface protein, e.g., a growth factor. Such binding assays are performed as known in the art.
[0163] In situ detection of antibody binding to the TIM-1 protein can be performed, for example, by immunofluorescence or immunoelectron microscopy.
For this purpose, a tissue specimen is removed from the patient, and a labeled antibody is applied to it, preferably by overlaying the antibody on a biological sample. This procedure also allows for determining the distribution of the marker gene product in the tissue examined. It will be apparent for those skilled in the art that a wide variety of histological methods are readily available for in situ detection.
Epitope Mapping [0169] The specific part of the protein immunogen recognized by an antibody may be determined by assaying the antibody reactivity to parts of the protein, for example an N terminal and C terminal half. The resulting reactive fragment can then be further dissected, assaying consecutively smaller parts of the immunogen with the antibody until the minimal reactive peptide is defined. Anti-TIM-1 mAb 2.70.2 was assayed for reactivity against overlapping peptides designed from the antigen sequence and was found to specifically recognize the amino acid sequence PLPRQNHE (SEQ ID NO:96) corresponding to amino acids 189-202 of the TIM-1 immunogen (SEQ ID NO:54). Furthermore using an alanine scanning technique, it has been determined that the second proline and the asparagine residues appear to be important for mAb 2.70.2 binding.
[0170] Alternatively, the epitope that is bound by the anti-TD)I-1 antibodies of the invention may be determined by subjecting the TIM-1 immunogen to SDS-PAGE
either in the absence or presence of a reduction agent and analyzed by immunoblotting.
Epitope mapping may also be performed using SELDI. SELDI ProteinChip (LumiCyte) arrays used to define sites of protein-protein interaction. TIM-1 protein antigen or fragments thereof may be specifically captured by antibodies covalently immobilized onto the PROTEINCHIP array
-48-surface. The bound antigens may be detected by a laser-induced desorption process and analyzed directly to determine their mass.
[0171] The epitope recognized by anti-TIM-1 antibodies described herein may be detelmined by exposing the PROTEINCHIP Array to a combinatorial library of random peptide 12-mer displayed on Filamentous phage (New England Biolabs). Antibody-bound phage are eluted and then amplified and taken through additional binding and amplification cycles to enrich the pool in favor of binding sequences. After three or four rounds, individual binding clones are further tested for binding by phage ELISA assays performed on antibody-coated wells and characterized by specific DNA sequencing of positive clones.
Examples [0172] The following examples, including the experiments conducted and results achieved are provided for illustrative purposes only and are not to be construed as limiting upon the invention described herein.
Example 1 Preparation of monoclonal antibodies that bind TIM-1 [0173] The soluble extracellular domain of TIM-1 was used as the immunogen to stimulate an immune response in XenoMouse animals. A DNA (CG57008-02), which encodes the amino acid sequence for the TIM-1 extracellular domain (minus the predicted N-terminal signal peptide) was subcloned to the baculovirus expression vector, pMelV5His (CuraGen Corp., New Haven, CT), expressed using the pBlueBac baculovirus expression system (Invitrogen Corp., Carlsbad, CA), and confirmed by Western blot analyses. The nucleotide sequence below encodes the polypeptide used to generate antibodies.
TCTGTAAAGGTTGGTGGAGAGGCAGGTCCATCTGTCACACTACCCTGCCACTACA
GTGGAGCTGTCACATCAATGTGCTGGAATAGAGGCTCATGTTCTCTATTCACATG
CCAAAATGGCATTGTCTGGACCAATGGAACCCACGTCACCTATCGGAAGGACAC
ACGCTATAAGCTATTGGGGGACCTTTCAAGAAGGGATGTCTCTTTGACCATAGAA
AATACAGCTGTGTCTGACAGTGGCGTATATTGTTGCCGTGTTGAGCACCGTGGGT
GGTTCAATGACATGAAAATCACCGTATCATTGGAGATTGTGCCACCCAAGGTCA
CGACTACTCCAATTGTCACAACTGTTCCAACCGTCACGACTGTTCGAACGAGCAC
CACTGTTCCAACGACAACGACTGTTCCAACGACAACTGTTCCAACAACAATGAG
CATTCCAACGACAACGACTGTTCCGACGACAATGACTGTTTCAACGACAACGAG
-49-CGTTCCAACGACAACGAGCATTCCAACAACAACAAGTGTTCCAGTGACAACAAC
GGTCTCTACCTTTGTTCCTCCAATGCCTTTGCCCAGGCAGAACCATGAACCAGTA
GCCACTTCACCATCTTCACCTCAGCCAGCAGAAACCCACCCTACGACACTGCAG
GGAGCAATAAGGAGAGAACCCACCAGCTCACCATTGTACTCTTACACAA_CAGAT
GGGAATGACACCGTGACAGAGTCTTCAGATGGCCTTTGGAATAACAATCAAACT
CAACTGTTCCTAGAACATAGTCTACTG (SEQ ID NO:53) [0174] The amino acid sequence encoded thereby is as follows:
SVI(VGGEAGPSVTLPCHYSGAVTSMCWNRGSCSLFTCQNGIVWTNGTHVTYRICDT
RYKLIGDL S RRD VS LTIENTAVSD S GVYC CRVEHRGWFNDMICITVS LE IVPP KVTTTP
IVTTVPTVTTVRTSTTVPTTTTVPTTTVPTTMS IPTTTTVPTTMTVS TTTS VPTTTS IPTT
TSVPVTTTVSTFVPPMPLPRQNHEPVATSPSSPQPAETHPTTLQGAMREPTSSPLYSY
TTDGNDTVTESSDGLWNNNQTQLFLEHSLL (SEQ ID NO:54) [0175] To facilitate purification of recombinant TIM-1, the expression construct can incorporate coding sequences for the V5 binding domain V5 and a HIS tag.
Fully human IgG2 and IgG4 monoclonal antibodies (mAb), directed against TIM-1 were generated from human antibody-producing XenoMousee strains engineered to be deficient in mouse antibody production and to contain the majority of the human antibody gene repertoire on megabase-sized fragments from the human heavy and kappa light chain loci as previously described in Yang et al., Cancer Res. (1999). Two XenoMouse strains, an hIgG2 (xmg-2) strain and an IgG4 (3C-1) strain, were immunized with the TIM-1 antigen (SEQ
ID NO: 54).
Both strains responded well to immunization (Tables 2 and 3).
-50-Table 2 Serum titer of XENOMOUSE hIgG, strain immunized with TIM-1 antigen.
Group 1: 5 mice (hIgG2 strain); mode of immunization = footpad Reactivity to TIM-1 Titers via hIgG
Mouae ID Bleed After 4 inj. Bleed After 6 inj.
M716-1 600,000 600,000 M716-2 600,000 500,000 M716-3 200,000 400,000 M716-4 300,000 200,000 M716-5 400,000 400,000 Negative Control 75 110 Positive Control 600,000 Table 3 Serum titer of XENOMOUSE Igat strain immunized with TIM-1 antigen Group 2: 5 mice (Ig04 strain); mode of immunization = footpad Reactivity to TIM-1 Titers via hIgG
Mouse ID Bleed After 4 inj. Bleed After 6 inj.
M326-2 15,000 73,000 M326-3 7,500 60,000 M329-1 27,000 30,000 M329-3 6,500 50,000 M337-1 2,500 16,000 Negative Control <100 90 Positive Control 600,000 [0176] Hybridoma cell lines were generated from the immunized mice.
Selected hybridomas designated 1.29, 1.37, 2.16, 2.17, 2.24, 2.45, 2.54 2.56, 2.59, 2.61, 2.70, and 2.76 (and subclones thereof) were further characterized. The antibodies produced by cell lines 1.29 and 1.37 possess fully human IgG2 heavy chains with human kappa light chains while those antibodies produced by cell lines 2.16, 2.17, 2.24, 2.45, 2.54 2.56, 2.59, 2.61, 2.70, and 2.76 possess fully human IgG4 heavy chains with human kappa light chains.
[0177] The amino acid sequences of the heavy chain variable domain regions of twelve anti-TIM-1 antibodies with their respective germline sequences are shown in Table 4
-51-below. The corresponding light chain variable domain regions amino acid sequence is shown in Table 5 below. "X" indicates any amino acid, preferably the gemiline sequence in the corresponding amino acid position. The CDRs (CDR1, CDR2, and CDR3) and FRs (FR1, FR2, and FR3) in the immunoglobulins are shown under the respective column headings.
(
-52-Table 4. Heavy Chain Analysis t..) o o mAb SEQ ID D FR1 CDR]. FR2 CDR2 FR3 CDR3 J .6.
,:TE5 NO:
ne .6.
55 Germline QVQLVESGGGVVQP GFTFSSYGMH
WVRQAPGKG VIWYDGSNKYYADSVKG RFTISRDNSKNTLYLQMN XXDY WGQGTLVTVSSA no GRSLRLSCAAS LEWVA
SLRAEDTAVYYCAR lµ.) 2.54 26 VH3-33/--/JH4b QVQLEQSGGGVVQP GFTFTNYGLH
WVRQAPGKG VIWYDGSHKFYADSVKG RFTISRDNSKNTLFLQMN DLDY WGQGTLVTVSSA
GRSLRLSCAAS LDWVA
SLRAEDTAVYYCTR
56 Germline QVQLVESGGGVVQP GFTFSSYGMH
WVRQAPGKG VIWYDGSNKYYADSVKG RFTISRDNSENTLYLQMN XXYDSSXXXYGMDV WGQGTTVTVSSA
GRSLRLSCAAS LEWVA
SLRAEDTAVYYCAX
2.76 46 VH3-33/03-22/JH6b XXXXEQSGGGVVQP
GFTFSSYGMY WVRQAPGKG VIWYDGSNKYYADSVKG RFTISRDNSKNTLYLQMN DFYDSSRYHYGMDV
WGQGTTVTVSSA
GRSLRLSCAAS LEWVA
SLRAEDTAVYYCAR
57 Germline QVQLQESGPGLVKP GGSISSGGYYWS WIRQHPGKG
YIYYSGSTYYNPSLKS RVTISVDTSKNQFSLKLS XXXXSSSWYXXFDY WGQGTLVTVSSA
SQTLSLTCTVS _LEWIG
SVTAADTAVYYCAR
2.59 34 VH4-31/D6-13/JH4b XXXXXQSGPRLVICP GGSISSDGYYWS WIRQHPGKG YIYYSGSTFYNPSLKS
RVAISVDTSKNQFSLKLS ESPHSSNWYSGFDC WGQGTLVTVSSA
SQTLSLTCTVS LEWIG
SVTAADTAVYYCAR n _ 58 Germline QVQLVESGGGVVQP GFTFSSYGMH
WVRQAPGKG VIWYDGSNKYYADSVKG RFTISRDNSKNTLYLQMN DYYDSSXXXXXFDY WGQGTLVTVSSA
o GRSLRLSCAAS LEWVA
SLRAEDTAVYYCAR iv 2.70 42 QVQLVESGGGVVQP GFIFSRYGMH
WVRQAPGKG VIWYDGSNKLYADSVKG RFTISRDNSKNTLYLQMN DYYDNSRHHWGFDY
WGQGTLVTVSSA in H
GRSLRLSCAAS LKWVA
SLRAEDTAVYYCAR li) in 2.24 18 QVQLEQSGGGVVQP GFTFSRYGMH
WVRQAPGKG VIWYDGSNKLYADSVKG RFTISRDNSKNTLYLQMN DYYDNSRHHWGFDY
WGQGTLVTVSSA iv SLRAEDTAVYYCAR op /JH4b 2.61 38 QVQLVEAGGGVVQP GFTFRSYGMH
WVRQAPGKG VIWYDGSNKYYTDSVKG RFTISRDNSKNTLYLQMN DYYDNSRHHWGFDY
WGQGTLVTVSSA iv GRSLRLSCAAS LKWVA
SLRAEDTAVYYCVR o o 2.56 30 QVQLVESGGGVVQP GFTFSSYGMH
WVRQAPGKG VIWYDGSHKYYADSVKG RFTISRDNSKNTLYLQMN DYYDTSRHHWGFDC
WGQGTLVTVSSA in GRSLRLSCAAS LEWVA
SLRAEDTAVYYSAR o .
ko 59 Germline EVQLVESGGGLVKP GFTFSNAWMS
WVRQAPGKG RIKSKTDGGTTDYAAPVKG RFTISRDDSENTLYLQMN XDXXXDY

GGSLRLSCAAS LEWVG
SLKTEDTAVYYCTX H
m 2.16 10 VH3-15/D3-16/JH4b XXXXEQSGGGVVKP
GFTFSNAWMT WVRQAPGKG RIKRRTDGGTTDYAAPVKG RFTISRDDSKNTLYLQMN VDNDVDY
WGQGTLVTVSSA
GGSLRLSCAAS LEWVG
NLKNEDTAVYYCTS
60 Germline QVQLQESGPGLVKP GGSVSSGGYYWS WIRQPPGKG
YIYYSGSTNYNPSLKS RVTISVDTSKNQFSLKLS XXXWXXXFDY
WGQGTLVTVSSA
SETLSLTCTVS LEWIG
SVTAADTAVYYCAR
1.29 2 VH4-61/D1-7/JH4b QVQLQESGPGLVKP GGSVSSGGYYWS WIRQPPGKG FIYYTGSTNYNPSLKS
RVSISVDTSKNQFSLKLS DYDWSFHFDY WGQGTLVTVSSA
SETLSLTCTVS LEWIG
SVTAADAAVYYCAR
61 Germaine EVQLVESGGGLVKP GFTFSNAWMS
WVRQAPGKG RIKSKTDGGTTDYAAPVKG RFTISRDDSKNTLYLQMN XXXSGDY
WGQGTLVTVSSA
GGSLRLSCAAS LEWVG
SLKTEDTAVYYCTT
IV
2.45 22 VH3-15/D6-19/JH4b XXXX.WSGGGLVKP
GFTFSNAWMT WVRQAPGKG RIKRKTDGGTTDYAAPVKG RFTISRDDSENTLYLQMN VDNSGDY
WGQGTLVTVSSA r) GGSLRLSCAAS LEWVG
SLETEDTAVYYCTT
62 Germline EVQLVESGGGLVQP GFTFSSYWMS
WVRQAPGKG NIKQDGSEKYYVDSVKG RFTISRDNAKNSLYLQMN XDY WGQGTLVTVSSA
GGSLRLSCAAS LEWVA
SLRAEDTAVYYCAR CP
lµ.) 1.37 6 VH3-7/--/JH4b EVQLVESGGGLVQP GFTFTNYWMS
WVRQAPGKG NIQQDGSEKYYVDSVRG RFTISRDNAKNSLYLQMN WDY WGQGTLVTVSSA

GGSLRLSCAAS LEWVA
SLRAEDSAVYYCAR .6.
63 -Germline EVQLVESGGGLVQP GFTFSSYSMN
WVRQAPGKG YISSSSSTIYYADSVKG RFTISRDNAKNSLYLQMN XFDY WGQGTLVTVSSA Ci5 GGSLRLSCAAS LEWVS
SLRDEDTAVYYCAX ne 2.17 14 VH3-48/--/JH4b QVQLEQSGGGLVQP GFTFSTYSMN
WVRQAPGKG YIRSSTSTIYYAESLKG RFTISSDNAKNSLYLQMN DFDY WGQGTLVTVSSA
CA

GGSLRLSCAAS LEWVS
SLRDEDTAVYYCAR lµ.)
-53-Table 5. Light(3bahlAnalysis t..) o mAb SEQ ID J FR1 CDRI FR2 CDR2 NO:
.6.
64 Germline EIVLTQSPGTLSLS RASQSVSSSYLA WYQQKPGQAPR GASSRAT
GIPDRFSGSGSGTDFTLTISRL QQYGSSXXLT FGGGTKVEIKR pc PGERATLSC LLIY
EPEDFAVYYC .6.
, 2.54 28 A27/JK4 ETQLTQSPGTLSLS RASQSVSNNYLA WYQQKPGQAPR GASSRAT
GIPDRFSGSGSGTDFTLTISRL QQYGSSLPLT FGGGTKVEIKR
(.4.) PGERVTLSC LLIY
EPEDCAECYC
65 Germline DIVMTQSPLSLPVT RSSQSLLHSNGYNWYLQKPGQSPQ LGSNRAS
GVPDRFSGSGSGTDFTLKISRV MQALQTXXT FGGGTKVEIKR
PGEPASISC YLD LLIY
EAEDVGVYYC
2.16 12 XXXLTQSPLSLPVT RSSQSLLHSNGYNWYLQKPGQSPQ LGSNRAS
GVPDRFSGSGSGTDFTLKISRV MQALQTPLT FGGGTKVDIKR

EAEDIGLYYC
2.45 24 XXXXTQSPLSLPVT RSSQSLLHSNGYN WYLQKPGQSPQ LGSNRAS
GVPDRFSGSGSGTDFTLKISRV MQALQTPLT FGGGTKVEIKR
PGEPASISC YLD LLIY
EAEDVGVYYC
66 Germline DIQMTQSPSSLSAS RASQGIRNDLG WYQQKPGKAPK AASSLQS
GVPSRFSGSGSGTEFTLTISSL LQHNSYPLT FGGGTKVEIKR
VGDRVTITC RLIY
QPEDFATYYC
1.29 4 A30/JK4 DIQMTQSPSSLSAS RASQGIRNDLG WYQQKPOKAPK AASSLQS

IGDRVTITC RLIY
QPEDFATYYC
o 67 Germline DIVMTQTPLSSPVT RSSQSLVHSDMTTWLQQRPGQPPR KISNRFS
GVPDRFSGSGAGTDFTLKISRV MQATQFPXIT FGQGTRLEIKR iv LGQPASISC YLS LLIY
EAEDVGVYYC in H
2.17 16 A23/J105 EIQLTQSPLSSPVT RSSQSLVHSDGDTWLQQRPGQPPR KISTRFS
GVPDRFSGSGAGTDFTLKISRV MQTTQIPQIT FGQGTRLEIKR ko LGQPASISC YLN LLIY
ETDDVGIYYC in iv 68 Germline DIQMTQSPSSLSAS RASQSISSYLN WYQQKPGKAPK AASSLQS
GVPSRFSGSGSGTDFTLTISSL QQSYSTPPT FGQGTKVEIKR op VGDRVTITC LLIY
QPEDFATYYC iv o 2.24 20 012/JK1 DIQLTQSPSSLSAS RASQSIYSYLN wYQQKpGKApKAASSLQS
GVPSRFSGSGSGTDFTLTISSL QQSYSTPPT FGQGTKVEIKR o VGDRVTITC LLIY
QPEDFATYYC in 69 Germline DIVMTQTPLSSPVT RSSQSLVHSDMTTWLQQRPGQPPR KISNRFS
GVPDRFSGSGAGTDFTLKISRV MQATQFPQT FGQGTKVEIKR o ko LGQPASISC YLS LLIY

1.37 8 A23/JK1 DIVMTQTPLSSTVI RSSQSLVHSDGNTWLQQRPGQPPR MISNRFS
GVPDRFSGSGAGTDFTLKISRV MQATESPQT FGQGTKVEIKR
H
M
LGQPASISC YLN LLIY
EAEDVGVYYC .
70 Germline DIVMTQTPLSLPVT RSSQSLLDSDDGNWYLQKPGQSPQ TLSYRAS
GVPDRFSGSGSGTDFTLKISRV MQRIEFPIT FGQGTRLEIKR
PGEPASISC TYLD LLIY
EAEDVGVYYC
2.70 44 DIVMTQTPLSLPVT RSSRSLLDSDDGNWYLQKPGQSPQ TLSYRAS
GVPDRFSGSGSGTDFTLKISRV MQRVEFPIT FGQGTRLEIKR
PGEPASISC TYLD LLIY
EAEDVGVYYC
2.56 32 01 EIVMTQTPLSLPVT RSSQSLLDSEDGNWYLQKPGQSPQ TLSHRAS
GVPDRFSGSGSGTDFTLKISRV MQRVEFPIT FGQGTRLEIKR

PGEPASISC TYLD LLIY
EAEDVGVYCC
2.76 48 XXXXTQCPLSLPVT RSSQSLLDSDDGNWYLQKPGQSPQ TVSYRAS
GVPDRFSGSGSGTDFTLKISRV MQRIEFPIT FGQGTRLEIKR
IV
PGEPASISC TYLD LLIY
EAEDVGVYYC
r) 71 Germline EIVLTQSPDFQSVT RASQSIGSSLH WYQQKPDQSPK YASQSFS
GVPSRFSGSGSGTDFTLTINSL HQSSSLPFT FGPGTKVDIKR
PKEKVTITC LLIK
EAEDAATYYC
_ 2.59 36 A26/JK3 XXXXTQSPDFQSVT RASQSIGSRLH WYQQKPDQSPK YASQSFS
GVPSRFSGSGSGTDFTLTINSL HQSSNLPFT FGPGTKVDIKR CP
l,..) PKEKVTITC LLIK

72 Germline DIQMTQSPSSLSAS RASQGIRNDLG WYQQKPGKAPK AASSLQS
GVPSRFSGSGSGTEFTLTISSL LQHNSYPXX FGQGTKLEIKR .6.
VGDRVTITC RLIY
QPEDFATYYC

-2.61 40 A30/JK2 DIQMTQSPSSRCAS RASQGIRNDLA WYQQKPGKAPK AASSLQS

VGDRVTITC RLIY
QPEDFAAYYC CA

l,..)
-54-[0178] Human antibody heavy chain VH3-33 was frequently selected in productive rearrangement for producing antibody successfully binding to TJA4-1. Any variants of a human antibody VH3-33 gemiline in a productive rearrangement making antibody to TIM-1 is within the scope of the invention. Other heavy chain V
regions selected in TIM-1 binding antibodies included: VH4-31, VH3-15, VH4-61, VH3-7 and VH3-48. The light chain V regions selected included: A27, A3, A30, A23, 012, 01, and A26.
It is understood that the Xi XenoMousee may be used to generate anti-TIM-1 antibodies utilizing lambda V regions.
[0179] The heavy chain variable domain germ line usage of the twelve anti-TIM-1 antibodies is shown in Table 6. The light chain variable domain germ line usage is shown in Table 7 (below).
-55-Table 6. Genii Line Usage of the Heavy Chain Variable Domain Regions _ tnAb V Heavy V #N' N D1 D1 Sequence #N's N D2 D2 #N's N JH J Constant CDR1 CDR2 CDR3 t.) Sequence s Sequen Sequence Region =
o ce .6.
---a-, oe _ .6.
2.16 VH3-15 TGTACC 5 TCA D3-16 CGATAA - N.A - - N.A - - N.A - - N.A - 7 TGACGTG JH4b (304-343) GACTAC G4 (344-529) 64-93 136-192 289-309 oe (1-285) GT (291-296) t.) c...) 2.70 VH3-33 GAGAGA 0 D3-22 TTACTATGAT - N.A - -N.A - - N.A - -N.A -15 AGACATCA JH4b (322-364) 11 1 GAC G4 (365-502) 70-99 142-192 289-330 (1-290) (291-306) AATAGT (SEQ CTGGGGG
_ ID NO: 73) (SEQ ID NO:
74) 2.59 VH4-3I GAGAGA 8 ATC D6-13 ATAGCAGCAA - N.A - - N.A - - N.A - - NA - 5 TCGGG JH4b (315-358) C I II GA G4 (359-545) 61-96 139-186 283-324 (2-284) CCC (293-309) CTGGTAC
_ TC (SEQ ID NO: 75) _ .
2.24 VH3-33 GAGAGA 0 D3-22 TTACTATGAT - NA - - N.A - - NA - - N.A -15 AGACATCA JH4b (328-370) 1 l'IGAC G4 (371-568) 76-105 148-198 295-336 (1-296) (297-312) AATAGT (SEQ CTGGGGG

ID NO: 76) (SEQ ID NO:
_ 77) o _ n.) 1.29 VH4-61 GAGAGA 5 TTA DI-7 ACTGGA -N.A - - N.A - - N.A - -N.A - 6 GCTTCC JH4b (311-355) ACTTTG G2 (356-491) 70-105 148-195 292-321 in H
(1-293) _ TO (299-304) ko _ ._ in 2.61 VH3-33 GAGAGA 0 D3-22 TTACTATGAT - N.A - - N.A - -N.A - - N.A -15 AGACATCA JH4b (328-370) 11'1GAC G4 (371-534) 76-105 148-198 295-336 "
(1-296) (297-312) AATAGT (SEQ CTGGGGG
op ID NO: 78) (SEQ ID NO:
n.) o 79) o in 2.76 VH3-33 TGCGAG 6 -GGA D3-22 CTATGATAGT - N.A - - N.A - - N.A - - N.A -7 CGTTACC JH6b (308-358) ACTACG G4 (359-544) - 64-93 136-186 283-324 o1 (1-281) 1 I 1 (288-300) AGT (SEQ ID
ko NO: 80) _ H
_ 2.54 VH3-33 GCGAGA - - - N.A - - N.A - -N.A - - N.A - - N.A - - N.A - 2 TC -JH4b (299-340) TTGACT G4 (341-537)- 76-105 ' 148-198 295-306 - cn (1-296) N.A N.A -_ 1.37 VH3-7 (7- GCGAGA - - -N.A - -N.A - -N.A
- -N.A - -N.A - - N.A - 3 TOG JH4b (304-343) GACTAC G2 (344-469) 82-111 154-300) N.A N.A -2.17 VH3-48 TGTGCG - - - NA - - N.A - . - NA -- NA - - NA - - NA - 5 CGGGA JH4b (297-340) CTTTGA G4 (341-538) - 76-105 (2-291) N.A N.A -IV
n 2.45 VH3-15 CCACAG 7 TCG D6-19 CAGTGG - N.A - - N.A - - N.A - - N.A - 0 .1114b (300-340) TGACTA 04 (341-526) 61-90 ' 133-189 286-306 1-3 (2-286) ATA (294-299) c4 A
t..) _ o -2.56 -VH3-33 GAGAGA 0 D3-22 TTACTATGAT - N.A - - N.A - - N.A - - N.A - 20 CGAGTCGG JH4b (322-364) 111 GAC 04 (365-527) 70-99 142-192 289-330 o .6.
(1-290) (291-301) A (SEQ ID NO: CATCACTG
-a-, 81) GGGG (SEQ
o oe 1D NO: 82) o t..)
-56-Table 7. Germ Line Usage of the Light Chain Variable Domain Regions mAb VL V Sequence #N's N JL J Sequence Constant Region 2.70 01 (46-348) TTTCCT 0 JK5 (349-385) ATCACC
IGKC (386-522) 115-165 211-231 328-354 0 .6.
2.59 A26 (1-272) TTTACC 0 JK3 (273-310) -ATTCAC
IGKC (311-450) 58-80 136-156 253-279 OC
2.24 012 (1-287) CCCTCC 0 JK1 (288-322) GACGTT
IGKC (323-472) 70-102 148-168 265-291 .6.
OC
1.29 A30 (46-331) ACCCTC 0 JK4 (332-367) TCACTT IGKC (368-504) 115-t4.) 2.56 01 (46-348) TTTCCT 0 JK5 (349-385) ATCACC
IGKC (386-521) 115-165 211-231 328-354 2.61 A30 (1-287) CCCTCC 3 CAG
31(2 (291-322) TTTTGG IGKC (323-470) 70-102 148-168 265-291 2.76 01 (1-290) GTTTCC 0 JK5 (291-328) GATCAC
IGKC (329-419) 58-108 154-174 271-297 1.37 A23 (43-344) TCCTCA 0 JK1 (345-379) GACGTT IGKC (380-454) 112-2.17 A23 (1-302) TCCTCA 1 A
JK5 (304-340) ATCACC IGKC (341-490) 70-117 163-183 280-309 2.54 A27 (1-286) GCTCAC 4 TCCC JK4 (291-328) GCTCAC
IGKC (329-480) 70-105 151-171 268-297 n 2.16 A3 (2-290) AACTCC 2 GC JK4 (293-328) TCACTT IGKC (329-447) 61-108 154-174 271-297 2.45 A3 (1-287) AACTCC 2 GC
JK4 (290-325) TCACTT IGKC (326-465) 58-105 151-171 268-294 o iv Ul H
l0 Ul IV
CO
IV

Ul I

l0 I
H
Ol .0 n 1-i cp t.., o o .6.
-,i-::--, o oe u, o t..,
-57-[0180] The sequences encoding monoclonal antibodies 1.29, 1.37, 2.16, 2.17, 2.24, 2.45, 2.54 2.56, 2.59, 2.61, 2.70, and 2.76, respectively, including the heavy chain nucleotide sequence (A), heavy chain amino acid sequence (B) and the light chain nucleotide sequence (C) with the encoded amino acid sequence (D) are provided in the sequence listing as summarized in Table 1 above. A particular monoclonal antibody, 2.70, was further subcloned and is designated 2.70.2, see Table 1.
Example 2 Antibody reactivity with membrane bound TIM-1 protein by FACS.
[0181] Fluorescent Activated Cell Sorter (FACS) analysis was performed to demonstrate the specificity of the anti-TIM-1 antibodies for cell membrane-bound TIM-1 antigen and to identify preferred antibodies for use as a therapeutic or diagnostic agent. The analysis was performed on two renal cancer cell lines, ACHN (ATCC#:CRL-1611) and CAKI-2 (ATCC#:HTB-47). A breast cancer cell line that does not express the TIM-antigen, BT549, was used as a control. Table 8 shows that both antibodies 2.59.2 and 2.70.2 specifically bound to TIM-1 antigen expressed on ACHN and CAKI-2 cells, but not antigen negative BT549 cells. Based on the Geo Mean Ratios normalized to the irrelevant antibody isotype control (pK16), ACHN cells had a higher cell surface expression of TIM-1 protein than CAKI-2 cells.
Table 8 Geo Mean Ratio (relative to negative control) Antibody BIN ACHN CAKI-2 BT549 2.59.2 1 15.2 7.7 1.4 2.70.2 6 19.4 8.8 1.8 1.29 1 17.9 1.2 2.16.1 2 7.9 1.5 2.56.2 5 12.2 1.5 2.45.1 8 4.3 1.1
-58-Example 3 Specificity of the anti-TIM-1 monoclonal antibodies [OM] The anti-TIM-1 antibodies bound specifically to TIM-1 protein but not an irrelevant protein in an ELISA assay. TIM-1 antigen (with a V5-HIS tag) specific binding results for four of the anti-TIM-1 monoclonal antibodies (1.29, 2.56.2, 2.59.2, and 2.45.1) as well as an isotype matched control mAb PK16.3 are shown in Figure 1. The X
axis depicts the antibodies used in the order listed above and the Y axis is the optical density. The respective binding of these antibodies to the irrelevant protein (also with a V5-HIS tag) is shown in Figure 2.
ELISA Protocol.
101831 A 96-well high protein binding ELISA plate (Corning Costar cat.
no.
3590) was coated with 50 L of the TIM-1 antigen at a concentration of 5 lig/mL diluted in coating buffer (0.1M Carbonate, pH9.5), and incubated overnight at 4 oC. The wells were then washed five times with 200-300 tit of 0.5% Tween-20Tm in PBS. Next, plates were blocked with 200IAL of assay diluent (Pharmingen, San Diego, CA, cat. no.
26411E) for at least 1 hour at room temperature. Anti-TIM-1 monoclonal antibodies were then diluted in assay diluent with the final concentrations of 7, 15, 31.3, 62.5, 125, 250, 500 and 1000 ng/mL. An anti-V5-HRP antibody was used at 1:1000 to detect the V5 containing peptide as the positive control for the ELISA. Plates were then washed again as described above. Next 50 L of each antibody dilution was added to the proper wells, then incubated for at least 2 hours at room temp. Plates were washed again as described above, then 50 1.1.1_, of secondary antibody (goat anti-human-HRP) was added at 1:1000 and allowed to incubate for 1 hour at room temp. Plates were washed again as described above then developed with 1001AL of TMB substrate solution/well (1:1 ratio of solution A+B) (Pharmingen, San Diego, CA, cat.
no. 2642KK). Finally, the reaction was stopped with 50 L sulfuric acid and the plates read at 450nm with a correction of 550nm.
-59-Example 4 Antibody Sequences [0184] In order to analyze structures of antibodies, as described herein, genes encoding the heavy and light chain fragments out of the particular hybridoma were cloned.
Gene cloning and sequencing was accomplished as follows. Poly(A)+ mRNA was isolated from approximately 2 X 105 hybridoma cells derived from immunized XenoMousee mice using a Fast-Track kit (Invitrogen). The generation of random primed cDNA was followed by PCR. Human VH or human Vi family specific variable domain primers (Marks et. al., 1991) or a universal human VH primer, MG-30 (CAGGTGCAGCTGGAGCAGTCIGG) (SEQ ID NO:83) were used in conjunction with primers specific for the human:
Cy2 constant region (MG-40d; 5'-GCT GAG GGA GTA GAG TCC TGA GGA-3' (SEQ ID NO:84));
Cy 1 constant region (HG1; 5' CAC ACC GCG GTC ACA TGG C (SEQ ID NO:85));
or Cy3 constant region (HG3; 5' CTA CTC TAG GGC ACC TGT CC (SEQ ID NO:86)) or the human CK constant domain (hicP2; as previously described in Green et al., 1994).
Sequences of human MAbs-derived heavy and kappa chain transcripts from hybridomas were obtained by direct sequencing of PCR products generated from poly(A) RNA using the primers described above. PCR products were also cloned into pCRII using a TA
cloning kit (Invitrogen) and both strands were sequenced using Prism dye-terminator sequencing kits and an ABI 377 sequencing machine. All sequences were analyzed by alignments to the "V BASE sequence directory" (Tomlinson et al., MRC Centre for Protein Engineering, Cambridge, UK) using MacVector and Geneworks software programs.
[0185] In each of Tables 4-7 above, CDR domains were determined in accordance with the Kabat numbering system. See Kabat, Sequences of Proteins of Immunological Interest (National Institutes of Health, Bethesda, Md. (1987 and 1991)).
-60-Example 5 Epito_pe binning and BiaCore affinity determination Epitope binning [01361 Certain antibodies, described herein were "binned" in accordance with the protocol described in U.S. Patent Application Publication No. 20030157730, published on August 21, 2003, entitled "Antibody Categorization Based on Binding Characteristics."
[0137] MxhIgG conjugated beads were prepared for coupling to primary antibody.
The volume of supernatant needed was calculated using the following formula:
(n+10) x 504 (where n = total number of samples on plate). Where the concentration was known, 0.5 g/mL was used. Bead stock was gently vortexed, then diluted in supernatant to a concentration of 2500 of each bead per well or 0.5X105 /mL and incubated on a shaker in the dark at room temperature overnight, or 2 hours if at a known concentration of 0.5p,g/mL.
Following aspiration, 50A of each bead was added to each well of a filter plate, then washed once by adding 1004/well wash buffer and aspirating. Antigen and controls were added to the filter plate 50A/well then covered and allowed to incubate in the dark for 1 hour on shaker. Following a wash step, a secondary unknown antibody was added at 50A/well using the same dilution (or concentration if known) as used for the primary antibody. The plates were then incubated in the dark for 2 hours at room temperature on shaker followed by a wash step. Next, 50A/well biotinylated mxhIgG diluted 1:500 was added and allowed to incubate in the dark for 1 hour on shaker at room temperature. Following a wash step, 50A/well Streptavidin-PE was added at 1:1000 and allowed to incubate in the dark for 15 minutes on shaker at room temperature. Following a wash step, each well was resuspended in 80A blocking buffer and read using a Luminex system.
[0188] Table 9 shows that the monoclonal antibodies generated belong to eight distinct bins. Antibodies bound to at least three distinct epitopes on the TIM-1 antigen.
Determination of anti-TIM-1 mAb affinity using BiaCore analysis [0189] BiaCore analysis was used to deteunine binding affinity of anti-antibody to TIM-1 antigen. The analysis was performed at 25 C using a BiaCore biosensor equipped with a research-grade CM5 sensor chip. A high-density goat a human
-61-antibody surface over a CM5 BiaCoree chip was prepared using routine amine coupling.
Antibody supernatents were diluted to ¨ 5 Ag/mL in HBS-P running buffer containing 100 Ag/mL BSA and 10 mg/mL carboxymethyldextran. The antibodies were then captured individually on a separate surface using a 2 minute contact time, and a 5 minute wash for stabilization of antibody baseline.
[0190] TIM-1 antigen was injected at 292 nM over each surface for 75 seconds, followed by a 3-minute dissociation. Double-referenced binding data were obtained by subtracting the signal from a control flow cell and subtracting the baseline drift of a buffer inject just prior to the TIM-1 injection. TIM-1 binding data for each mAb were normalized for the amount of mAb captured on each surface. The normalized, drift-corrected responses were also measured. The kinetic analysis results of anti-TIM-1 mAB binding at 25 C are listed in Table 9 below.
Table 9 Competition Bins and Kips for TIM-1-specific mAbs Bin Antibody Affinity nM
by BIAcore 2.59 0.38 1.29 3.64 2 2.16 0.79 3 2.17 2.42 1.37 2.78 4 2.76 0.57 2.61 1.0 2.24 2.42 2.56 1.1 6 2.70 2.71 7 2.54 3.35 8 2.45 1.15
-62-Example 6 Epitope Mapping [0191] Anti-TIM-1 mAb 2.70.2 was assayed for reactivity against overlapping peptides designed from the TIM-1 antigen sequence. Assay plates were coated with the TIM-1 fragment peptides, using irrelevant peptide or no peptide as controls. Anti-TIM-1 mAb 2.70.2 was added to the plates, incubated, washed and then bound antibody was detected using anti-human Ig HRP conjugate. Human antibody not specific to TIM-1, an isotype control antibody or no antibody served as controls. Results showed that mAb 2.70.2 specifically reacted with a peptide having the amino acid sequence PMPLPRQNHEPVAT
(SEQ ID NO:87), corresponding to amino acids 189-202 of the TIM-1 immunogen (SEQ ID
NO: 54).
[0192] Specificity of mAb 2.70.2 was further defined by assaying against the following peptides:
A) PMPLPRQNHEPVAT (SEQ lD NO:87) B) PMPLPRQNHEPV (SEQ ID NO:88) C) PMPLPRQNHE (SEQ iD NO:89) D) PMPLPRQN (SEQ ID NO:90) E) PMPLPR (SEQ ID NO:91) F) PLPRQNHEPVAT (SEQ ID NO:92) G) PRQNHEPVAT (SEQ ID NO:93) H) QNHEPVAT (SEQ ID NO:94) I) HEPVAT (SEQ ID NO:95) [0193] Results showed mAb 2.70.2 specifically bound to peptides A, B, C, and F, narrowing the antibody epitope to PLPRNHE (SEQ ID NO:96) [0194] As shown in Table 10, synthetic peptides were made in which each amino acid residue of the epitope was replace with an alanine and were assayed for reactivity with mAb 2.70.2. In this experiment, the third proline and the asparagines residues were determined to be critical for mAb 2.70.2 binding. Furthermore, assays of peptides with additional N or C terminal residues removed showed mAb 2.70.2 binding was retained by the minimal epitope LPRQNH (SEQ ID NO:97)
-63-Table 10 SEQ ID mAb 2.70.2 NO: Reactivity P MP L P R QNHE 89 +
P MP AP R QN HE 98 +

P MP L P AQNHE 100 +
P MP L P R ANHE 101 +

P MP L P R QNAE 103 +
P LP R QNHE 104 +
L P R QNHE 105 +
P LP R QNHE 106 +
L P R QNHE 107 +
Example 7 Immunohistochemical (IHC) analysis of TIM-1 expression in nounal and tumor tissues [0195] Immunohistochemical (IHC) analysis of TIM-1 expression in normal and tumor tissue specimens was performed with techniques known in the art.
Biotinylated fully human anti-TIM-1 antibodies 2.59.2, 2.16.1 and 2.45.1 were analyzed.
Streptavidin-HRP
was used for detection.
[0196] Briefly, tissues were deparaffinized using conventional techniques, and then processed using a heat-induced epitope retrieval process to reveal antigenic epitopes within the tissue sample. Sections were incubated with 10% normal goat serum for minutes. Normal goat serum solution was drained and wiped to remove excess solution.
Sections were incubated with the biotinylated anti-TIM-1 mAb at 5 ttg/mL for 30 minutes at 25 C, and washed thoroughly with PBS. After incubation with streptavidin-HRP
conjugate , for 10 minutes, a solution of diaminobenzidine (DAB) was applied onto the sections to visualize the immunoreactivity. For the isotype control, sections were incubated with a
-64-biotinylated isotype matched negative control mAb at 5 i_tg/rnt for 30 minutes at 25 C instead of biotinylated anti-TIM-1 mAb. The results of the IHC studies are summarized in Tables 11 and 12.
[0197] The specimens were graded on a scale of 0-3, with a score of 1+
indicating that the staining is above that observed in control tissues stained with an isotype control irrelevant antibody. The corresponding histological specimens from one renal tumor and the pancreatic tumor are shown in Figure 3 (A and B). In addition to these the renal and pancreatic tumors, specimens from head and neck cancer, ovarian cancer, gastric cancer, melanoma, lymphoma, prostate cancer, liver cancer, breast cancer, lung cancer, bladder cancer, colon cancer, esophageal cancer, and brain cancer, as well the corresponding normal tissues were stained with anti-TIM-1 mAb 2.59.2. Overall, renal cancer tissue samples and pancreatic cancer tissue samples highly positive when stained with anti-TIM-1 mAb 2.59.2.
No staining in normal tissues was seen. These results indicate that TB/I-1 is a marker of cancer in these tissues and that anti-TIM-1 mAb can be used to differentiate cancers from normal tissues and to target TIM-1 expressing cells in vivo.
Table 11 Immunohistology Renal tumors expression of TIM-1 protein detected by anti-TIM-1 mAb 2.59.2 Specimen Cell Type Histology Score 1 Malignant cells Not known 0 1 Other Not cell associated 2 2 Malignant cells Clear Cell 2 3 Malignant cells Clear Cell 0 4 Malignant cells Clear Cell 3 Malignant cells Clear Cell 2 (occasional) 6 Malignant cells Not known 2 7 Malignant cells Clear Cell 2 8 Malignant cells Clear Cell 0 9 Malignant cells Clear Cell 2 (occasional) Malignant cells Clear Cell 1-2 11 Malignant cells Not known 3 (many) 12 Malignant cells Clear Cell 1-2 12 Other Not cell associated 2 13 Malignant cells Clear Cell 2 (occasional) 14 Malignant cells Clear Cell 1-2
-65-15 Malignant cells Clear Cell 3-4 16 Malignant cells Not known 1-2 17 Malignant cells Not known 4 (occasional) 18 Malignant cells Not known 1-2 19 Malignant cells Clear Cell 0 20 Malignant cells Clear Cell 3-4 21 Malignant cells Clear Cell 2 (occasional) 22 Malignant cells Clear Cell 3 23 Malignant cells Clear Cell 2 24 Malignant cells Not known 3-4 occasional 25 Malignant cells Not known 2-3 26 Malignant cells Not known 3 27 Malignant cells Clear Cell 2 27 Other Not cell associated 2 28 Malignant cells Not known 2 29 Malignant cells Clear Cell 2-3 30 Malignant cells Clear Cell 2 31 Malignant cells Clear Cell 2-3 32 Malignant cells Clear Cell 0 33 Malignant cells Clear Cell 0 34 Malignant cells Clear Cell 2 34 Other Not cell associated 2 35 Malignant cells Clear Cell 2-3 36 Malignant cells Clear Cell 3 37 Malignant cells Not known 3 38 Malignant cells Clear Cell 3 39 Malignant cells Not known 2 40 Malignant cells Clear Cell 2-3 Table 12 Normal Human Tissue Immunohistology with anti-TIM-1 mAb 2.59.2 Tissue Score Specimen 1 Specimen 2 Adrenal Cortex 0 0 Adrenal Medulla 0 1 Bladder: Smooth muscle 0 0 Bladder: Transitional Epithelium 3 0 Brain cortex: Blia 0 0 Brain cortex: Neurons 0 0 Breast: Epithelium 0 0 Breast: Stroma 0 0 Colon: Epithelium 0 0
-66-Colon: Ganglia 0 NA
Colon: Inflammatory compartment 3-4 (occasional) 3 (occasional) Colon: Smooth muscle 1 (occasional) 0 Heart: Cardiac myocytes 0 0 Kidney cortex: Glomeruli 2-3 Kidney cortex: Tubular epithelium 2 2-3 Kidney medulla:Tubular 2 0 epithelium Kidney medulla: other NA 2-3 Liver: Bile duct epithelium 0 0 Liver: Hepatocytes 1-2 1 Liver: Kupffer cells 0 0 Lung :Airway epithelium 0 0 Lung: Alveolar macrophages 2 (occasional)-3 2-3 (occasional) Lung: other 3 NA
Lung: Pneumocytes 2-3 (occasional) 2-3 (occasional) Ovary: Follicle 2 (occasional) 1-2 Ovary: Stroma 1 1 (occasional) Pancreas: Acinar epithelium 0 1 (occasional) Pancreas:Ductal epithelium 0 0 Pancreas:Islets of Langerhans 0 0 Placenta: Stroma 0 0 Placenta:Trophoblasts 0 0 Prostate: Fibromuscular stroma 0 0 Prostate: Glandular epithelium 0 0 Skeletal muscle: Myocytes 0 0 Skin: Dermis 0 0 Skin: Epidermis 0 0 Small intestine: Epithelium 0 0 Small intestine: Ganglion 0 0 Small intestine: Inflammatory 0 0 comp& tment Small intestine: Smooth muscle 0 0 cells Spleen: Red pulp 0 2 (rare) Spleen: white pulp 0 0 Stomach: Epithelium 0 0 Stomach: Smooth Muscle Cells 0 0 Tstis: Leydig cells 2 1-2 Testis: Seminiferous epithelium 1 2 Thymus: Epithelium 0 0 Thymus: Lymphocytes 2 (rare) 2 (occasional) Thyroid: Follicular epithelium 0 0 Tonsil: Epithelium 0 0
-67-Tonsil: Lymphocytes 3 (occasional) 2 (occasional) Uterus: Endometrium 0 0 Uterus: Myometrium 0 0 Example 8 Antibody mediated toxin killing [0198] A clonogenic assay as described in the art was used to determine whether primary antibodies can induce cancer cell death when used in combination with a saporin toxin conjugated secondary antibody reagent. KohIs and Lappi, Biotechniques, 28(1):162-5 (2000).
Assay Protocol [0199] ACHN and BT549 cells were plated onto flat bottom tissue culture plates at a density of 3000 cells per well. On day 2 or when cells reached ¨25%
confluency, 100 ng/well secondary mAb-toxin (goat anti-human IgG-saporin; Advanced Targeting Systems; HUM-ZAP; cat. no. IT-22) was added. A positive control anti-EGFR
antibody, mAb 2.7.2, mAb 2.59.2, or an isotype control mAb was then added to each well at the desired concentration (typically 1 to 500 ng/mL). On day 5, the cells were trypsinized, transferred to a 150 mm tissue culture dish, and incubated at 37 C. Plates were examined daily. On days 10-12, all plates were Giemsa stained and colonies on the plates were counted.
Plating efficiency was determined by comparing the number of cells prior to transfer to 150 mm plates to the number of colonies that eventually formed., [0200] The percent viability in antigen positive ACHN and antigen negative BT549 cell lines are presented in Figure 4 and Figure 5 respectively. In this study, the cytotoxic chemotherapy reagent 5 Flurouracil (5-FU) was used as the positive control and induced almost complete killing, whereas the saporin conjugated-goat anti-human secondary antibody alone had no effect. A monoclonal antibody (NeoMarkers MS-269-PABX) generated against the EGF receptor expressed by both cell lines was used to demonstrate primary antibody and secondary antibody- saporin conjugate specific killing.
The results indicate that both cell lines were susceptible to EGFR mAb mediated toxin killing at 100
-68-ng/mL. At the same dose, both the anti-TIM-1 mAb 2.59.2 and the anti-TIM-1 mAb 2.70.2 induced over 90% ACHN cell death as compared to 0% BT549 cell death.
Antibody toxin conjugate mediated killing: Clonogenic Assay [0201] CAKI-1 and BT549 cells were plated onto flat bottom tissue culture plates at a density of 3000 cells per well. On day 2 or when cells reach ¨25%
confluency, various concentrations (typically 1 to 1000 ng/ml) of unconjugated and Auristatin E
(AE)-conjugated mAb, which included anti-EGFR, anti-TIM-1 mAb 2.7.2, anti-TIM-1 mAb 2.59.2 or isotype control mAb, were added to cells. Each of these antibodies was conjugated to AE. The monoclonal antibody (NeoMarkers MS-269-PABX) generated against the EGF
receptor, which is expressed by both cell lines, was used as a positive control to demonstrate specific killing mediated by AE-conjugated antibody. On day 5, the cells were trypsinized, transferred to a 150 mm tissue culture dish, and incubated at 37 C. Plates were examined daily. On days 10-12, all plates were Giemsa stained and colonies on the plates were counted.
Plating efficiency was determined by counting the cells prior to transfer to 150 mm plates and compared to the number of colonies that eventually formed.
[0202] The percent viability in antigen positive CAKI-1 and antigen negative BT549 cell lines are presented in Figures 6 and 7, respectively.
[0203] The results indicate that unconjugated and AE-conjugated isotype control mAb had no effect on growth of both CAKI-1 and BT549 cells. However, both cell lines were susceptible to AE-EGFR mAb mediated toxin killing in a dose-dependent fashion. At the maximum dose, both anti-TIM-1 mAbs (2.59.2 and 2.70.2) induced over 90 %

cell death when compared to their unconjugated counterparts. The response was dose dependent. At the same dose range, both anti-TIM-1 mAbs 2.59.2 and 2.70.2 did not affect the survival of BT549 cells.
Example 9 Human Tumor Xenograft Growth Delay Assay [0204] A
tumor growth inhibition model is used according to standard testing methods. Geran et al., Cancer Chemother. Rep. 3:1-104 (1972). Athymic nude mice (nu/nu)
-69-are implanted with either tumor cells or tumor fragments from an existing host, in particular, renal (CaKi-1) or ovarian (OVCAR) carcinoma tumor fragments are used. These animals are then treated with an anti-TIM-1 antibody immunotoxin conjugate, for example, mAb 2.70.2 AE conjugate at doses ranging from 1 to 20 mg/kg body weight, twice weekly for a period of 2 weeks. Tumor volume for treated animals is assessed and compared to untreated control tumors, thus determining the tumor growth delay.
[0205] After reaching a volume of 100 mm3 animals are randomized and individually identified in groups of 5 individuals per cage. Protein or antibody of interest is administered via conventional routes (intraperitoneal, subcutaneous, intravenous, or intramuscular) for a period of 2 weeks. Twice weekly, the animals are evaluated for tumor size using calipers. Daily individual animal weights are recorded throughout the dosing period and twice weekly thereafter. Tumor volume is determined using the formula: Tumor volume (in mm3) = (length x width x height) x 0.536. The volume determinations for the treated groups are compared to the untreated tumor bearing control group. The difference in time for the treated tumors to reach specific volumes is calculated for 500 1000, 1500 and 2000 mm3. Body weights are evaluated for changes when compared to untreated tumor bearing control animals. Data are reported as tumor growth in volume plotted against time.
Body weights for each experimental group are also plotted in graph form.
[0206] Results show that the treatment is well tolerated by the mice.
Treatment with anti-TIM-1 mAb AE conjugate inhibits tumor growth of established CaKi-1 and OVCAR tumors.
Example 10 Treatment of Renal Carcinoma with anti-TIM-1 antibodies [0207] A patient in need of treatment for a renal carcinoma is given an intravenous injection of anti-TIM-1 antibodies coupled to a cytotoxic chemotherapic agent or radiotherapic agent. The progress of the patient is monitored and additional administrations of anti-TIM-1 antibodies are given as needed to inhibit growth of the renal carcinoma.
Following such treatment, the level of carcinoma in the patient is decreased.
-70-Example 11 FACS analysis of expression of TIM-1 protein on CD4+ T cells [0203] Mononuclear cells were isolated from human blood diluted 1:1 in PBS, by spinning over Ficoll for 20 minutes. The mononuclear cells were washed twice at 1000 rpm with PBS ¨Mg and Ca and re-suspended in Miltenyi buffer (Miltenyi Biotec Inc., Auburn, CA); PBS, 0.5% BSA, 5 mM EDTA at approximately 108 cells/mL. 20 ILIL of CD4 Miltenyi beads were added per 107 cells and incubated for 15 minutes on ice. Cells were washed with a 10-fold excess volume of Miltenyi buffer. A positive selection column (type VS+) (Miltenyi Biotec Inc., Auburn, CA) was washed with 3 mL of Miltenyi buffer.
The pelleted cells were re-suspended at 108 cells per mL of Miltenyi buffer and applied to the washed VS
column. The column was then washed three times with 3 mL of Miltenyi buffer.
Following this, the VS column was removed from the magnetic field and CD4+ cells were eluted from the column with 5 mL of Miltenyi buffer. Isolated CD4+ lymphocytes were pelleted and re-suspended in DMEM 5% FCS plus additives (non-essential amino acids, sodium pyruvate, mercaptoethanol, glutamine, penicillin, and streptomycin) at 106 cells/mL.
1x106 freshly isolated resting CD4+ T cells were transferred into flow cytometry tubes and washed with 2 mL/tube FACS staining buffer (FSB) containing PBS, 1% BSA and 0.05% NaN3.
Cells were spun down and supernatant removed. Cells were blocked with 20% goat serum in FSB
for 30 minutes on ice. Cells were washed as above and incubated with 10 g/mL
of primary human anti-TIM-1 mAb or control PK16.3 mAb in FSB (200 iu,L) for 45 minutes on ice followed by washing. Secondary goat anti-human PE conjugated antibody was added at 1:50 dilution for 45 minutes on ice in the dark, washed, resuspended in 500 j.iL of PBS containing 1% formaldehyde and kept at 4 C until flow cytometry analysis was performed.
[0209] FACS analysis was performed to determine the expression of TIM-protein as detected with five anti-TIM-1 monoclonal antibodies (2.59.2, 1.29, 2.70.2, 2.56.2, 2.45.1) on human and mouse resting CD4+ T cells, as well as human activated and human polarized CD4+ T cells. These analyses demonstrate that freshly isolated resting human
-71-CD4+ T cells do not express TIM-1, while a major fraction of polarized human Th2 and Thl cells do express TIM-1.
[0210] FACS
Analysis of the Expression of the T114-1 protein on human CD4+
Th2 cells using five anti-TIM-1 monoclonal antibodies is shown in Table 13.
The experiment is described in the left-hand column and the labeled antibody is specified along the top row. Data is reported as the geometric mean of the fluorescence intensity.
Table 13 FACS Analysis of the Expression of the TIM-1 protein on human CD4+ Th2 cells Geometric mean of fluorescence intensity Experiment Control Anti-TIM-1 mAb PK16.3 1.29 2.45.1 2.56.2 2.59.2 2.70.2 Resting Human 4.6 4.7 5.1 6 4.9 N/A
CD4+ T cells Polarized 8.4 22.3 42.4 564.1 22 27.8 Human CD4+
Th2 Cells [0211] Table 14 demonstrates that over the course of 5 days, continual stimulation of T cells results in an increase in TIM-1 expression, as measured by anti-TIM-1 mAb 2.70.2, as compared to the control PK16.3 antibody.
Furthermore, addition of matrix metalloproteinase inhibitor (MMPI) did not measurably increase TIM-1 expression, demonstrating that the receptor is not shed from T cells under these experimental conditions.
Thus, expression of the TIM-1 protein and specific antibody binding is specific to activated Thl and Th2 cells, which in turn, are characteristic of inflammatory response, specifically asthma.
-72-Table 14 Percent of activated T cells that express TIM-1 Day 0 Day 1 lay 2 &ay 4 Pay 5 -WIMP' 1 3 3 1 1 Control PK16.3 + MAN 1 2 6 2 2 2.70.2 + MMPI 1 10 14 10 19 Example 12 Cytokine assays 10212] IL-4, IL-5, IL-10, IL-13, and IFN-7 production levels by activated Thl and Th2 cell were measured in culture supernatants treated with anti-TIM-1 antibodies using standard ELISA protocols. Cytokine production by Thl or Th2 cells treated with anti-TIM-1 antibodies was compared to Thl or Th2 cells treated with the control PK16.3 antibody. In addition, the following samples were run in parallel as internal controls: i) anti-CD3 treated Thl or Th2 cells, where no cytokine production is expected because of the absence of co-stimulation, ii) anti-CD3/anti-CD28 stimulated Thl or Th2 cells, expected to show detectable cytokine production, and iii) untreated Thl or Th2 cells. CD4+ T cells were isolated as described in the Example above. Isolated CD4+ lymphocytes were then spun down and re-suspended in DMEM 5% FCS plus additives (non-essential amino acids, sodium pyruvate, mercaptoethanol, glutamine, penicillin, and streptomycin) at 106 cells/mL.
Falcon 6-well non-tissue culture treated plates were pre-coated overnight with anti-CD3 (2 1.1g/mL) and anti-CD28 (10 jug/mL) (600 jiL total in Dulbecco's PBS) overnight at 4 C. The plates were washed with PBS and CD4+ lymphocytes were suspended at 500,000 cells/mL in Th2 medium: DMEM+ 10% FCS plus supplements and IL-2 5ng/mL, IL-4 5 ng/mL, anti-IFN

gamma 51.tg/mL and cells were stimulated 4-6 days at 37 C and 5% CO2 in the presence of 5
-73-p,g/mL of mAb recognizing the TIM-1 protein or isotype matched negative control mAb PK16.3.
[0213] In another set of experiments, CD4+ lymphocytes were suspended at 500,000 cells/mL in Thl medium: DMEM+ 10% FCS plus supplements and IL-2 5 ng/mL, 1112 5 ng/mL, anti-IL-4 5p,g/mL and stimulated 4-6 days 37 C temp and 5% CO2 in the presence of 5 ps/mL TIM-1 or isotype matched control mAb PK16.3. Cells were washed two times in DMEM and resuspended in DMEM, 10% FCS plus supplements and 2 ng/mL
IL-2 (500,000 cells/mL) in the presence of 5 g/mL TIM-1 mAb or control PK16.3 mAb and cultured (rested) for 4-6 days at 37 C and 5% CO2. The process of activation and resting was repeated at least once more as described above with the addition of anti-CD95L (anti-FAS ligand) to prevent FAS-mediated apoptosis of cells. Falcon 96-well non-tissue culture treated plates pre-coated overnight with anti-CD3 mAb at 500 ng/mL and costimulatory molecule B7H2 (B7 homolog 2) 5 g/mL were washed and 100 p,L of TIM-1 mAb treated Thl or Th2 (200,000 cells) added per well. After 3 days of culture, the supernatants were removed and I1-4, 11-5, 11-10, I1-13, and IFNy levels were determined by ELISA

(Pharmingen, San Diego, CA or R&D Systems, Minneapolis, MN).
[0214] As demonstrated below, anti-TIM-1 mAb significantly inhibited release of the tested cytokines by Thl and Th2 cells (see Figures 8-17). Results where inhibition of cytokine production is significant (p=.02-.008), are marked on the bar graphs with an asterisk. Tables 15 and 16 summarize the bar graphs in Figures 8-17.
-74-Table 15 Cytokine Inhibition in CD4+ Thl cells using anti-TIM-1 antibodies in two independent human donors Experiments that demonstrate significant inhibition of cytokine production are marked with an asterisk: P= 0.01 to 0.05 *; P=0.005 to 0.009 **; P=0.001 to 0.004 ***
Donor 12+17 Percentage of Control Antibody Cytokines Anti-TIM-1 IL-5 1L-4 IL-10 IL-13 INF y mAbs Till 2.56.2 100.17 28.49 * 63.76 * 86.45 93.69 2.45.1 90.23 39.78 * 83.98 96.25 100.6 1.29 94.63 81.05 60.77 ** 73.95 *** 93.51 2.59.2 66.62 * 31.40 * 68.99 * 54.5 *** 128.12 Table 16 Cytokine Inhibition in CD4+ Th2 cells using anti-TIM-1 antibodies in two independent human donors Experiments that demonstrate significant inhibition of cytokine production are marked with an asterisk: P= 0.01 to 0.05 *;P=0.005 to 0.009 **; P-0.001 to 0.004 ***
Donor 12+17 Percentage of Control Antibody Cytokines Anti-TIM-1 IL-5 IL-4 IL-10 IL-13 INF y mAbs TH2 2.56.2 112.07 103.46 93.97 86.45 88.30 2.45.1 148.7 25.66 *** 55.97 * 86.81 25.66 *
1.29 80.26 112.54 44.45 * 48.91 ** 112.54 2.59.2 23.62* 19.17** 43.86* 43.71*** 19.18*
[0215] A summary of Th2 cytokine inhibition data obtained from multiple experiments with different donors is provided in Table 17. Each experiment used purified CD4+ cells isolated from whole blood samples from two independent donors.
Cytokine
-75-production is reported as the percent of cytokine production detected using the control PK16.3 mAb. The anti-TIM-1 mAb used in each experiment is specified along the bottom row. Results that report significant cytokine inhibition are underlined in Table 17 below.
The use of "ND" indicates that the experiment was not performed. These results do reflect donor dependent variability but show that mAbs 2.59.2 and 1.29 reproducibly block one or more of the Th2 cytokines.
Table 17 Summary of Cytokine Inhibition using anti-TIM-1 mAbs 2.59.2 and 1.29 in 5 independent human donor groups Results of experiments that report inhibition greater than 50% of that seen using the control PK16.3 antibody are underlined.
Donor ID 12+17 12+14 13+14 14 12 Cytokine Anti-TIM-1 Anti-TIM-1 mAb 1.29 mAb 2.59.2 Example 13 Construction, expression and purification of anti-TIM-1 scFv.
[0216] The VL and VH domains of mAb 2.70 were used to make a scFv construct. The sequence of the anti-TIM-1 scFv was synthesized by methods known in the art.
[0217] The nucleotide sequence of anti-TIM-1 scFv is as follows:
ATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGC
CGGCCATGGCCGATATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCCC
-76-TGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCGGAGCCTCTTGGATAGTGAT
GATGGAAACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG
CTCCTGATCTACACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTG
GCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGG
ATGTTGGAGTTTATTACTGCATGCAACGTGTAGAGTTTCCTATCACCTTCGGCCA
AGGGACACGACTGGAGATTAAACTTTCCGCGGACGATGCGAAAAAGGATGCTGC
GAAGAAAGATGACGCTAAGAAAGACGATGCTAAAAAGGACCTCCAGGTGCAGC
TGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCT
GTGCAGCGTCTGGATTCATCTTCAGTCGCTATGGCATGCACTGGGTCCGCCAGGC
TCCAGGCAAGGGGCTGAAATGGGTGGCAGTTATATGGTATGATGGAAGTAATAA
ACTCTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAA
GAACACGCTGTATCTGCAAATGAACAG CCTGAGAGC CGAGGACACGGCTGTGTA
TTACTGTGCGAGAGATTACTATGATAATAGTAGACATCACTGGGGGTTTGACTAC
TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTAGCGATTATAAGGACGAT
GATGACAAATAG (SEQ ID NO:108) [0218] The amino acid sequence of mature anti-TIM-1 scFv is as follows:
DIVMTQTPLSLPVTP GEPASIS CRS S RSLLD S DD GNTYLDWYLQKPGQSP QLLIYTLSY
RAS GVPDRFS GS GS GTDFTLKISRVEAEDVGVYYCMQRVEFPITFGQGTRLEIKLSAD
DAKKDAAKKDDAKKDDAKKDLQVQLVE S GGGVVQPGRSLRLS GAAS GFIFSRYGM
HWVRQAPGKGLKWVAVIWYDGSNKLYAD SVKGRFTISRDNSKNTLYLQMNSLRAE
DTAVYYCARDYYDNSRHHWGFDYWGQGTLVTVSSASDYKDDDDK (SEQ ID
NO:109) [0219] The synthesized DNA can be inserted into the pET-20b(+) expression vector, for periplasmic expression in E. coli. Cells are grown and the periplasmic proteins prepared using standard protocols. Purification of the anti-TIM-1 scFv is achieved using an anti-FLAG M2 affinity column as per the manufacturer's directions. The predicted molecular weight of the mature protein is 30222.4 daltons. This purified scFv is used in the assays described below to test for biological activity. The scFv construct is comprised of a signal peptide (SP), VL (VL1) derived from mAb 2.70, a linker (L4) based on the 25 amino acid linker 205C, the VH (VH1) derived from mAb 2.70, and a Tag (in this case the FLAG tag).
It will be obvious to those skilled in the art that other SP, linker and tag sequences could be utilized to get the same activity as the anti-TIM-1 scFv antibody described herein.
-77-Example 14 Construction, expression and purification of anti-TIM-1 and anti-CD3 bispecific scFv1 [02201 The basic formula for the construction of this therapeutic protein is as follows:
SP1 ¨ VL1 ¨ Ll ¨ VH1 ¨ L2 ¨ VH2 ¨ L3 ¨ VL2 ¨ Tag [0221] The signal peptide SP1 is the same as IgG kappa signal peptide from Medical Research Council (MRC) Centre for Protein Engineering, University of Cambridge, UK.
[0222] Other signal peptides can also be used and will be obvious to those skilled in the art. This protein is designed to be expressed from mammalian cells. The predicted molecular weight of the mature cleaved protein is 54833.3 dalton. Li corresponds to the (Gly4Ser)3 linker, while linker 2 (L2) corresponds to the short linker sequence: GGGGS. L3 is an 18 amino acid linker. VH2 corresponds to the anti-CD3 variable heavy chain domain from Genbank (accession number CAE85148) while VL1 corresponds to the anti-CD3 variable light chain domain from Genbank (accession number CAE85148). The tag being used for this construct is a His tag to facilitate purification and detection of this novel protein.
Standard protocols are used to express and purify this His tagged protein, which is tested for activity and tumor cell killing in the protocols described below.
[0223] The amino acid and nucleic acid numbering for the components comprising the anti-TIM-1 and anti-CD3 bispecific scFv1 is as follows:
SP: -20 to ¨1 aa; -60 to ¨1 nt VL1: 1-113 aa; 1-339nt Li: 114-128 aa; 340-384nt VH1: 129-251 aa; 385-753nt L2: 252-256 aa; 754-768nt VH2: 257-375 aa; 769-1125nt L3: 376-393 aa; 1126-1179nt VL2: 394-499 aa; 1180-1497nt Tag: 500-505 aa; 1498-1515nt
-78-[0224] The nucleotide sequence of anti-UM4 and anti-CD3 bispecific scFv1 is as follows:
ATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA

GCCGGC CTCCATCTCCTG CAGGTCTAGTC GGAGCCTCTTGGATAGTGATGATGGA
AACACCTATTTGGACTGGTACCTGCAGAA GCCAGGGCAGTCTCCACAGCTCCTG
ATCTACACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTG
GGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTG
GAGTTTATTACTGCATGCAACGTGTAGAGTTTCCTATCACCTTCGGCCAAGGGAC
ACGACTGGAGATTAAAGGTGGTGGTGGTTCTGGCGGCGGCGGCTCCGGTGGTGG
TGGTTCCCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAG
GTCCCTGAGACTCTCCTGTGCAGCGTCTGGATTCATCTTCAGTCGCTATGGCATG
CACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGAAATGGGTGGCAGTTATATGG
TATGATGGAAGTAATAAACTCTATGCAGACTCCGTGAAGGGCCGATTCACCATCT
CCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCG
AGGACACGGCTGTGTATTACTGTGCGAGAGATTACTATGATAATAGTAGACATC
ACTGGGGGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGAG
GTGGTGGATCCGATATCAAACTGCAGCAGTCAGGGGCTGAACTGGCAAGACCTG
GGGCCTCAGTGAAGATGTCCTGCAAGACTTCTGGCTACACCTTTACTAGGTACAC
GATGCACTGGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATTGGATACAT
TAATCCTAGCCGTGGTTATACTAATTACAATCAGAAGTTCAAGGACAAGGCCAC
ATTGACTACAGACAAATCCTCCAGCACAGCCTACATGCAACTGAGCAGCCTGAC
ATCTGAGGACTCTGCAGTCTATTACTGTGCAAGATATTATGATGATCATTACTGC
CTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAGTCGAAGGTGGA
AGTGGAGGTTCTGGTGGAAGTGGAGGTTCAGGTGGAGTCGACGACATTCAGCTG
ACCCAGTCTCCAGCAATCATGTCTGCATCTCCAGGGGAGAAGGTCACCATGACCT
GCAGAGCCAGTTCAAGTGTAAGTTACATGAACTGGTACCAGCAGAAGTCAGGCA
CCTCCCCCAAAAGATGGATTTATGACACATCCAAAGTGGCTTCTGGAGTCCCTTA
TCGCTTCAGTGGCAGTGGGTCTGGGACCTCATACTCTCTCACAATCAGCAGCATG
GAGGCTGAAGATGCTGCCACTTATTACTGCCAACAGTGGAGTAGTAACCCGCTC
ACGTTCGGTGCTGGGACCAAGCTGGAGCTGAAATAG (SEQ ID NO:110) [0225] The protein sequence of mature anti-TIM-1 and anti-CD3 bispecific scFv1 is as follows:
DIVMTQTPLSLPVTP GEPASIS CRS SRSLLD SDD GNTYLDWYLQKP GQ SP QLLIYTLSY
RASGVPDRFS GS GS GTDFTLKISRVEAEDVGVYYCMQRVEFPITFGQGTRLEIKGGGG
SGGGGSGGGGSQVQLVESGGGVVQPGRSLRLS CAASGFIFSRYGMHWVRQAPGKGL
KWVAVIWYDGSNKLYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARDY
YDNSRHHWGFDYWGQGTLVTVS S GGGGSDIKLQQS GAELARPGASVKMS CKTSGY
TFTRYTMHWVKQRP GQGLEVVIGYINP SRGYTNYNQKFICDKATLTTDKS S STAYMQL
S S LT SED SAVYYCARYYDDHYCLDYW GQGTTLTVS SVEGGS GGSGGS GGS GGVDDI
QLTQ SP APM SASPGEKVTMTCRAS S SVSYMNWYQQKS GTSPKRWIYDTSKVASGVP
-79-YRFSGSGSGTSYSLTISSMEAEDAATYYCQQWSSNPLTFGAGTKLELK (SEQ ID
NO:111) Example 15 Construction, expression and purification of anti-TIM-1 and anti-CD3 bispecific scFv2:
[0226] The basic formula for the construction of this novel therapeutic protein is as follows:
SP1 ¨ VL1 ¨ L4 ¨ VH1 ¨ L2 ¨ VH2 ¨ L4 ¨ VL2 ¨ Tag [0227] The signal peptide SP1 is IgG kappa signal peptide VKIII A27 from Medical Research Council (MRC) Centre for Protein Engineering, University of Cambridge, UK. For more information see mrc-cpe.cam.ac.uk/ALIGNMENTS.php?menu=901. Other signal peptides and linkers could also be used to get additional biologically active bispecific single chain antibodies. The protein being described in this example is also designed to be expressed from mammalian cells and is similar to the anti-TIM-1 and anti-CD3 bispecific scFv1, except that it utilizes a different linker as indicated in the basic formula above (L4, as described earlier), and that a Flag tag is used instead of the His tag as in the first example.
[0229] The predicted molecular weight of the mature cleaved protein is 58070.0 dalton. The tag being used for this construct is a FLAG tag to facilitate purification and detection of this novel protein. Standard protocols are used to express this secreted protein and purify it, which is tested for activity and tumor cell killing in the protocols described below.
[0230] The amino acid and nucleic acid numbering for the components comprising the anti-TIM-1 and anti-CD3 bispecific scFv2 is as follows:
SP: -20 to ¨1 aa; -60 to ¨int VL1: 1-113 aa; 1-339nt Li: 114-138 aa; 340-414nt VH1: 139-261 aa; 415-783nt L2: 262-266 aa; 784-798nt VH2: 267-385 aa; 799-1155nt L3: 386-410 aa; 1156-1230nt
-80-VL2: 411-516 aa; 1231-1548nt Tag: 517-524 aa; 1549-1572nt [0231] The nucleotide sequence of anti-TIM-1 and anti-CD3 bispecific scFv2 is as follows:
ATGG.AAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA
CCGGAGATATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGA
GCCGGCCTCCATCTCCTGCAGGTCTAGTCGGAGCCTCTTGGATAGTGATGATGGA
AACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTG
ATCTACACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTG
GGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTG
GAGTTTATTACTGCATGCAACGTGTAGAGTTTCCTATCACCTTCGGCCAAGGGAC
ACGACTGGAGATTAAACTTTCCGCGGACGATGCGAAAAAGGATGCTGCGAAGAA
AGATGACGCTAAGAAAGACGATGCTAAAAAGGACCTGCAGGTGCAGCTGGTGG
AGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAG
CGTCTGGATTCATCTTCAGTCGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGG
CAAGGGGCTGAAATGGGTGGCAGTTATATGGTATGATGGAAGTAATAAACTCTA
TGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACAC
GCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTG
TGCGAGAGATTACTATGATAATAGTAGACATCACTGGGGGTTTGACTACTGGGG
CCAGGGAACCCTGGTCACCGTCTCCTCAGGAGGTGGTGGATCCGATATCAAACT
GCAGCAGTCAGGGGCTGAACTGGCAAGACCTGGGGCCTCAGTGAAGATGTCCTG
CAAGACTTCTGGCTACACCTTTACTAGGTACACGATGCACTGGGTAAAACAGAG
GCCTGGACAGGGTCTGGAATGGATTGGATACATTAATCCTAGCCGTGGTTATACT
AATTACAATCAGAAGTTCAAGGACAAGGCCACATTGACTACAGACAAATCCTCC
AGCACAGCCTACATGCAACTGAGCAGCCTGACATCTGAGGACTCTGCAGTCTATT
ACTGTGCAAGATATTATGATGATCATTACTGCCTTGACTACTGGGGCCAAGGCAC
CACTCTCACAGTCTCCTCACTTTCCGCGGACGATGCGAAAAAGGATGCTGCGAA
GAAAGATGACGCTAAGAAAGACGATGCTAAAAAGGACCTGGACATTCAGCTGAC
CCAGTCTCCAGCAATCATGTCTGCATCTCCAGGGGAGAAGGTCACCATGACCTGC
AGAGCCAGTTCAAGTGTAAGTTACATGAACTGGTACCAGCAGAAGTCAGGCACC
TCCCCCAAAAGATGGATTTATGACACATCCAAAGTGGCTTCTGGAGTCCCTTATC

GGCTGAAGATGCTGCCACTTATTACTGCCAACAGTGGAGTAGTAACCCGCTCAC
GTTCGGTGCTGGGACCAAGCTGGAGCTGAAAGATTATAAGGACGATGATGACAA
ATAG (SEQ ID NO:112) [0232] The protein sequence of mature anti-TIM-1 and anti-CD3 bispecific scFv2 is as follows:
[0233]
DIVMTQTPLSLPVTP GEPAS IS CRS S RSLLDSDDGNTYLDWYLQKP
-81-GQSPQLLIYTLSYRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQRVEFPITF
GQGTRLEIKLSADDAKKDAAKKDDAKKDDAKKDLQVQLVESGGGVVQPGRSLRLS
CAASGFIFSRYGMHWVRQAPGKGLKWVAVIWYDGSNKLYADSVKGRFTISRDNSK
NTLYLQMNSLRAEDTAVYYCARDYYDNSR_HHWGFDYWGQGTLVTVSSGGGGSDI
KLQQSGAELARPGASVKMSCKTSGYTFTRYTMHWV KQRPGQGLEWIGYMSRGYT
NYNQKFICDKATLTTDKSSSTAYMQLSSLTSEDSAVYYCARYYDDHYCLDYWGQGT
TLTVSSLSADDAKKDAAKKDDAKKDDAICKDLDIQLTQSPAEVISASPGEKVTMTCRA
S S SVSYMNWYQQKS GTSPKRWIYDTSKVAS GVPYRFS GS GS GT SYS LTIS S MEAEDA
ATYYCQQWSSNPLTFGAGTKLELKDYKDDDDK (SEQ ID NO:113) Example 16 Anti-TIM-1 scFv species biological activity ELISA Analysis:
[0234] To determine if the anti-TIM-1 and anti-CD3 bispecific scFv1 and scFv2 antibodies bind to specific antigen, ELISA analysis is performed. lug/ml of specific antigen (TIM-1 antigen (CG57008-02) is bound to ELISA plates overnight in carbonate/bicarbonate buffer (pH approximately 9.2-9.4). Plates are blocked with assay diluent buffer purchased from Pharmingen San Diego, CA), and various concentrations of the anti-TIM-1 scFv bispecific antibodies are added for 1 hour at room temp. Plates are washed in 0.01% Tween 2OTM in PBS, followed by addition of HRP-conjugated mAb to either the 6-His tag (Invitrogen, Carlsbad, CA) or the FLAG peptide tag or (Sigma, St. Louis, MO) in assay diluent for 60 minutes at room temperature. Color is developed with TMB substrate (Phanningen), and the reaction stopped with H2SO4. Plates are read at A450 nm, and the O.D. value taken as a measure of protein binding.
FACS analysis [0235] Binding of the anti-TIM-1 and anti-CD3 bispecific scFv1 and scFv2 antibodies, as well as the anti-TIM-I scFv antibody to cells expressing the antigens recognized by the anti-TIM-1 human mAbs is examined by FACS analysis. Cells (such as ACHN) are washed in PBS and resuspended in FACS buffer consisting of ice cold PBS with addition of 1% BSA or 1% FBS. The resuspended cells are then incubated on ice with various concentrations of the bispecific antibody for 30 minutes. Cells are washed to remove
-82-non-bound antibody. Bound antibody is detected by binding of a secondary labeled mAb (phycoerythrin or FITC labeled) that specifically recognizes the 6-his tag or the FLAG-tag that is engineered on the bispecific antibody sequence. Cells are washed and analyzed for binding of the anti-tag mAb by FACS analysis. Binding of bispecific mAb plus anti-tag mAb is compared to binding of the anti-tag mAb alone.
Cytotoxicity analysis [0236] To determine if the bispecific antibody has functional activity as defined by the ability of the bispecific to target T cells to TIM-1 expressing normal or tumor cells, the bispecific antibody is tested in a Cytotoxicity assay. T cells are obtained from the low density cells derived from centrifugation of blood over density separation medium (specific density 1.077). T cells can be used in a heterogeneous mix from the peripheral blood ' mononuclear cell fraction (which also contains B cells, NK cells and monocytes) or further purified from the low-density cells using MACS separation and negative or positive selection. Killing in assays with T cells derived from the blood directly will have less cytolytic activity than cells that have been stimulated in vitro with PHA, cytokines, activating monoclonal antibodies or other stimulators of polyclonal T cell activation.
Therefore, these activators will be used to further boost the activity of T cells in the functional assays. Many variations of cytotoxicity assays are available. Cytotoxicity assays measure the release of natural products of the cells metabolism upon lysis, such as LDH. Other assays are based around labeling cells with various agents such as radioactive chromium (51Cr), DELFIA
BATDA, CSFE or similar labeling agents and detecting release or change in live cells bound by the agent.
[0237] DELFIA cytotoxicity assays (PerkinElmer Life and Analytical Sciences, Inc. Boston, MA) offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.
-83-Target cells are resuspended to a concentration of 2x106/ml. 10 jil of DELFIA
BATDA was mixed in a tube with 2 ml of target cells according to the manufacturers instructions. Various concentrations of T cells are added to a fixed concentration of labeled target cells (5000 cells per well) in 96 well U-bottom plates, and incubated for at least 2 hours at 37 C. The plates are spun at approximately 200g, followed by the aspiration of 20 pi of supernatant, which was then added to a europium solution (200 gl) in a separate plate. The plate is incubated for 15 minutes at room temperature, followed by analysis on a SAFIRE (Tecan, Maennedorf, Switzerland) according to the manufacturer's instructions. Signal in the test wells are compared to signal in 100% lysis well (10% lysis buffer in place of T cells) and cell with medium alone (spontaneous release), and % specific lysis is calculated from the formula %specific lysis = (test ¨ spontaneous release)/100% lysis x100.
BIAcore kinetic analysis of scFv constructs [0238]
Kinetic measurements to determine the affinity for the scFv constructs (monomer as well as bispecific, containing at least 1 scFv moiety binding to TIM-1) are measured using the methods described earlier for the whole antibodies of this invention.
scFv-containing antibody protein affinities to TIM-1 are expected to be within a factor of 10, i.e. between 0.271 ¨ 27.1 nM, of the affinity given for mAb 2.70.
Example 17 Ability of anti-TIM-1 mAb to inhibit the proliferation of human ovary carcinoma cells [0239]
Several fully human monoclonal antibody clones were isolated from the immunizations described above and their ability to inhibit the proliferative potential of OVCAR-5 (human ovary carcinoma) cells was analyzed using the 5-bromo-2-deoxyuridine (BrdU) incorporation assay (described in International Patent Application No.
WO
01/25433).
[0240] In the BrdU assay, OVCAR-5 cancer cells (Manassas, VA) were cultured in Dulbeccos Modification of Eagles Medium (DMEM) supplemented with 10% fetal bovine serum or 10% calf serum respectively. The ovarian cancer cell line was grown to confluence
-84-at 37 C in 10% CO2/air. Cells were then starved in DMEM for 24 hours. Enriched conditioned medium was added (10 4/100 'IL of culture) for 18 hours. BrdU (10 01) was then added and incubated with the cells for 5 hours. BrdU incorporation was assayed by colorimetric immunoassay according to the manufacturer's specifications (Boehringer Mannheim, Indianapolis, IN).
[0241] The capability of various human anti-TIM-1 monoclonal antibodies to neutralize was assessed. The results provided in Figures 18A-17T are presented in a bar graph founat to assist in comparing the levels of BrdU incorporation in OVCAR5 cells upon exposure to various human anti-TIM-lmonoclonal antibodies described herein. As positive and negative controls, OVCAR5 cells were cultured in the presence of either complete media (complete) or restricted serum-containing media (starved). In addition, the monoclonal antibody PK16.3 was included as a negative treatment control representing a human IgG
antibody of irrelevant specificity. Human anti-TIM-1 monoclonal antibodies described herein were used at varying doses (10-1000 ng/mL) as compared to a control run utilizing varying concentrations.
Example 18 Antibody conjugate studies 0242j Additional antibody conjugate studies were performed using the plant toxin saporin conjugated to anti-TIM-1-specific mABs (1.29 and 2.56.2) and various irrelevant antibodies, including, PK16.3 (Figures 19A-19C). Additional negative controls included anti-TIM-1-specific mAB 2.56.2 and irrelevant antibody PK16.3 without toxin (Figure 19D). Four cancer cell lines, three kidney cancer cell lines (ACHN, CAKI, and 786-0) and one breast cancer cell line (BT549), were treated for 72 hours with saporin-antibody conjugates or antibodies alone, after which time BrdU was added to monitor proliferation over a 24 hour period. The results are described in Figures 19A-20C for the kidney cancer cell lines and Figure 19D for the breast cancer cell line. All three kidney cancer cell lines were sensitive to treatment with saporin-TIM-1-specific antibody conjugates as evidenced by a measurable decrease in BrdU incorporation. Treatment of the same cell lines with conjugated irrelevant antibodies had little or no effect demonstrating antigen dependent
-85-antiproliferative effects. The same studies performed with the BT549 cell line showed that the TIM-1-specific antibody 2.56.2 showed no antiproliferative effect either alone or when conjugated to saporin. The negative controls for these studies appeared to work well with no cytotoxic effects Example 19 Sequences [0243] Below are sequences related to monoclonal antibodies against TIM-1.
With regard to the amino acid sequences, bold indicates framework regions, underlining indicates CDR regions, and italics indicates constant regions.
Anti-TIM-1 mAb 1.29 [0244] Nucleotide sequence of heavy chain variable region and a portion of constant region:
5' TGGGTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC
TTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCGTCAGCAGTGGT
GGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATT
GGGTTTATCTATTACACTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAG
TCTCCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGT
GACCGCTGCGGACGCGGCCGTGTATTACTGTGCGAGAGATTATGACTGGAGCTT
CCACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCACC
AAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGC
ACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTG
TCGTGGAACTCAGGCGCTCT3' (SEQ ID NO:1) [0245] Amino acid sequence of heavy chain variable region and a portion of constant region encoded by SEQ ID NO:1:
WVLSQVQLQES GPGLVKPSE TL SL TC TVS GGSVS S GGYYW S WIRQPPGKGLEWI
GFIYYTGSTNYNP SLKSRVSISVDT SKNQFSLI(LSSVTAADAAVYYCARDYDWSFH
FDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGA
(SEQ NO:114) [0246j Nucleotide sequence of light chain variable region and a portion of constant region:
-86-5' CAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACATCCAG
ATGACCCAGTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCA
CTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAAC
CAGGGAAAGCCCCTAAGCGCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGG
TCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCA
GCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAGCATAATAGTTA
CCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGC
ACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCC
TCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA
AGGTGGATAACGCC3' (SEQ ID NO:3) [0247] Amino acid sequence of light chain variable region and a portion of constant region encoded by SEQ ID NO:3:
QLLGLLLLWFPGARCDIQMTQSPSSLSASIGDRVTITCRASQURNDLGWYQQKPG
KAPICRLIYAASSLOSGVPSRFSGSGSGTEFTLTISSLQPEDFATYYCLQHNSYPLTF
GGGTKVEIKRTVAAPSVHFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNA (SEQ
ID NO:115) Anti-TIM-1 mAb 1.37 Nucleotide sequence of heavy chain variable region and a portion of constant region:
5' CAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGG
GTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTACTAACTATTGGATG
AGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTGGCCAACATACAG
CAAGATGGAAGTGAGAAATACTATGTGGACTCTGTGAGGGGCCGATTCACCATC
TCCAGAGACAACGCCAAGAACT CACTGTATCTGCAAATGAACAGCCTGAGAGCC
GAGGACTCGGCTGTGTATTACTGTGCGAGATGGGACTACTGGGGCCAGGGAACC
CTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCGC
CCTGCTCCAGGAGCAC CT CCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGG
ACTACTTCCCCGAACCGGTGAGCGGTGTCGTGGAAC3' (SEQ ID NO:5) [02491 Amino acid sequence of heavy chain variable region and a portion of constant region encoded by SEQ ID NO:5:
QCEVQLVESGGGLVQPGGSLRLSCAASGFTFTNYWMSWVRQAPGKGLEWVANI
OODGSEKYYVDSVRGRFTISRDNAKNSLYLQMNSLRAEDSAVYYCARWDYWGQ
GTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCL VKDYFPEPVSGVVE (SEQ
ID
NO:116)
-87-[0250] Nucleotide sequence of light chain variable region and a portion of constant region:
5' CTTCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGGGGATATTGTGATG
ACCCAGACTCCACTCTCCTCAACTGTCATCCTTGGACAGCCGGCCTCCATCTCCT
GCAGGTCTAGTCAAAGCCTCGTACACAGTGATGGAAACACCTACTTGAATTGGC
TTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATATGATTTCTAACCG
GTTCTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCAC
ACTGAAAATCAGCAGGGTGGAAGCTGAGGATGTCGGGGTTTATTACTGCATGCA
AGCTACAGAATCTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAA
TCTGGAAGGGCCTCTGTTG3' (SEQ ID NO:7) [0251] Amino acid sequence of light chain variable region and a portion of constant region encoded by SEQ ID NO:7:
LLGLLMLWVPGSSGDIVMTQTPLSSTVILGQPASISCRSSQSLVHSDGNTYLNWLQQ
RPGQPPRLLIYMISNRFSGVPDRFSGSGAGTDFTLKISRVEAEDVGVYYCMQATE
SPQTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGRASV (SEQ ID NO:117) Anti-TIM-1 mAb 2.16 [0252] Nucleotide sequence of heavy chain variable region and a portion of constant:
5' GAGCAGTCGGGGGGAGGCGTGGTAAAGCCTGGGGGGTCTCTTAGACTCTCCTG
TGCAGCCTCTGGATTCACTTTCAGTAACGCCTGGATGACCTGGGTCCGCCAGGCT
CCAGGGAAGGGGCTGGAGTGGGTTGGCCGTATTAAAAGGAGAACTGATGGTGGG
ACAACAGACTACGCTGCACCCGTGAAAGGCAGATTCACCATCTCAAGAGATGAT
TCAAAAAACACGCTGTATCTGCAAATGAACAACCTGAAAAACGAGGACACAGCC
GTGTATTACTGTACCTCAGTCGATAATGACGTGGACTACTGGGGCCAGGGAACC
CTGGTCACCGTCTCCTCAGCTTCCACCAAGGGCCCATCCGTCTTCCCCCTGGCGC
CCTGCTCCAGGAGCACCTCCGAGAGCACAGCCGCCCTGGGCTGCCTGGTCAAGG
ACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCG
GCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT3' (SEQ ID NO:9) [0253] Amino acid sequence of heavy chain variable region and a portiorj of constant region encoded by SEQ ID NO:9:
XXXXEQSGGGVVKPGGSLELSCAASGFTFSNAWMTWVRQAPGKGLEWVGRIICR
RTDGGTTDYAAPVKGRFTISRDDSK.NTLYLQMNNLKNEDTAVYYCTSVDNDVDY
WGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCL VKDYFPEPVTVSWNSGALTSG
VHTFPAVLQSSGL (SEQ ID NO :118)
-88-[0254] Nucleotide sequence of light chain variable region and a portion of constant region:

TCCTGCAGGTCTAGTCAGAGC CTCCTGCATAGTAATGGATACAACTATTTGGATT
GGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAA
TCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTT
ACACTGAAAATCAGCAGAGTGGAGGCTGAGGATATTGGTCTTTATTACTGCATGC
AAGCTCTACAAACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAAC
GAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCC
AAAGTACAG3' (SEQ ID NO:11) [0255] Amino acid sequence of light chain variable region and a portion of constant region encoded by SEQ ID NO:11:
XXXLTQSPLSLPVTPGEPASISCRS SQSLLHSNGYNYLDWYLQKPGQSPQLLIYLG
SNRASGVPDRFSGSGSGTDFTLKISRVEAEDIGLYYCMQALQTPLTEGGGTKVDI
KRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQ (SEQ ID NO:119) Anti-TIM-1 mAb 2.17 [0256] Nucleotide sequence of heavy chain variable region and a portion of constant region:
5' CAGGTGCAGCTGGAGCAGTCGGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCT
GAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATAGCATGAACTGG
GTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTAGAAGTAGT
ACTAGTACCATATACTATGCAGAGTCCCTGAAGGGCCGATTCACCATCTCCAGCG
ACAATGCCAAGAATTCACTATATCTGCAAATGAACAGCCTGAGAGACGAGGACA
CGGCTGTGTATTACTGTGCGCGGGACTTTGACTACTGGGGCCAGGGAACCCTGGT
CACCGTCTCCTCAGCTTCCACCAAGGGCCCATCCGTCTTCCCCCTGGCGCCCTGC
TCCAGGAGCACCTCCGAGAGCACAGCCGCCCTGGGCTGCCTGGTCAAGGACTAC
TTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTG
CACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCA3' (SEQ
ID NO:13) [0257] Amino acid sequence of heavy chain variable region and a portion of constant region encoded by SEQ ID NO:13:
-89-QVQLEQSGGGLVQPGGSLRLSCAASGFTFSTYSMNWVRQAPGKGLEWVSYIRSS
TSTIYYAESLKGRFTISSDNAKNSLYLQMNSLRDEDTAVYYCARDFDYWGQGTL
VTVSSASTKGPSVFPLAPCSRSTSESTAAL GCLVKDYFPEPVTVSWNSGALTSGVHTFPAV
LQSSGLYSLS (SEQ ID NO:120) [0253] Nucleotide sequence of light chain variable region and a portion of constant region:
5' GAAATCCAGCTGACTCAGTCTCCACTCTCCTCACCTGTCACCCTTGGACAGCCG
GCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGATGGAGACACCT
ACTTGAATTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAA
GATTTCTACCCGGTTCTCTGGGGTCCCTGACAGATTCAGTGGCAGTGGGGCAGGG
ACAGATTTCACACTGAAAATCAGCAGGGTGGAGACTGACGATGTCGGGATTTAT
TACTGCATGCAAACTACACAAATTCCTCAAATCACCTTCGGCCAAGGGACACGA
CTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG
ATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTA
TCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTA3' (SEQ ID NO:15) [0259] Amino acid sequence of light chain variable region and a portion of constant region encoded by SEQ ID NO:15:
EIQLTQSPLSSPVTLGQPASISCRSSOSLVHSDGDTYLNWLQQRPGQPPRLLIYKIS
TRFS GVPDRFS GS GAGTDFTL KISRVE TDDVGIYYCMQTTOIP OITFGQ GTRLEIK
RTVAAPSVFIFPPSDEQLKSGTASVVCLLIVNFYPREAKVQWKVDNALQSG (SEQ ID
NO:121) Anti-TIM-1 mAb 2.24 [0260] Nucleotide sequence of heavy chain variable region and a portion of constant region:
5' CAGGTGCAGCTGGAGCAGTCGGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCT
GAGACTCTCCTGTGCAGCGTCTGGATTCACCTTCAGTCGCTATGGCATGCACTGG
GTCCGCCAGGCTCCAGGCAAGGGGCTGAAATGGGTGGCAGTTATATGGTATGAT
GGAAGTAATAAACTCTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGA
GACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGAC
ACGGCTGTGTATTACTGTGCGAGAGATTACTATGATAATAGTAGACATCACTGGG
GGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTTCCACCAA
GGGCCCATCCGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACA
GCCGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGT
GGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGT
CCTCAGGACTCTACTCCCTCAGCA (SEQ ID NO:17)
-90-[0261] Amino acid sequence of heavy chain variable region and a portion of constant region encoded by SEQ ID NO:17:
OVQLEQSGGGVVQPGRSI,RIL CAASGFTFSRYGMHWVRQAPGKGLKWVAVIW
YDGSNKLYADSVKGRFTISRDNSENTLYLQIVINSL DTAVYYCARDYYDNSRH
HWGFDYWGQGTINTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSW
NSGALTSGVHTFPAVLQSSGLYSLS (SEQ ID NO:122) [02621 Nucleotide sequence of light chain variable region and a portion of constant region:
'GACATCCAGCTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGA
GTCACCATCACTTGCCGGGCAAGTCAGAGTATTTATAGTTATTTAAATTGGTATC
AGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGC
AAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCT
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGT
TACAGTACCCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACT
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTG
GAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT
ACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTA3' (SEQ ID NO:19) [0263] Amino acid sequence of light chain variable region and a portion of constant region encoded by SEQ ID NO:19:
DIQL/MT/LQSPSSLSASVGDRVTITCRASQSTYSYLNWYQQKPGKAPKLLIYAASS
LQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPPTFGQGTKVEIKRTV
AAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSG (SEQ ID NO:123) Anti-TIM-1 mAb 2.45 [0264] Nucleotide sequence of heavy chain variable region and a portion of constant region:
5'CAGTCGGGGGGAGGCTTGGTAAAGCCTGGGGGGTCCCTTAGACTCTCCTGTGC
AGCCTCTGGATTCACTTTCAGTAACGCCTGGATGACCTGGGTCCGCCAGGCTCCA
GGGAAGGGGCTGGAGTGGGTTGGCCGTATTAAAAGGAAAACTGATGGTGGGAC
AACAGACTACGCTGCACCCGTGAAAGGCAGATTCACCATCTCAAGAGATGATTC
AGAAAACACGCTGTATCTGCAAATGAACAGCCTGGAAACCGAGGACACAGCCGT
GTATTACTGTACCACAGTCGATAACAGTGGTGACTACTGGGGCCAGGGAACCCT
GGTCACCGTCTCCTCAGCTTCCACCAAGGGCCCATCCGTCTTCCCCCTGGCGCCC
TGCTCCAGGAGCACCTCCGAGAGCACAGCCGCCCTGGGCTGCCTGGTCAAGGAC
-91-TACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGC
GTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTCT3' (SEQ ID NO:21) [0265] Amino acid sequence of heavy chain variable region and a portion of constant region encoded by SEQ ID NO:21:
1(3=Q GGGINKPGGSLRL SC AASGFTF SNAWMTWVRQAPGKGLENINGRIKR
KTDGGTTDYAAPVKGRFTISRDDSENTLYLQMNSLETEDTAVYYCTTVDNSGDY
WGQGTINTYSSASTKGPSVFPLAPCSRSTSESTAALGCLUDYFPEPVTVSWNSGALTSG
VHTFPAVLQSSGLS (SEQ ID NO:124.) [0266] Nucleotide sequence of light chain variable region and a portion of constant region:
S'ACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCC
TGCAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGT
ACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCG
GGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTAC
ACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCA
AGCTCTACAAACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAA
TCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCA
AAGTACAGTGGAAGGTGGATAACGCCCTCA3' (SEQ ID NO:23) [0267] Amino acid sequence of light chain variable region and a portion of constant region encoded by SEQ ID NO:23:
VOCXT Q SPL SLPVTPGEPASIS CRS S QSLLHSNGYNYLDWYLQKPGQSPQLLIYLG
SNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQALQTPLTFGGGTKVEI
KRTVAAPSVF1FPPSDEQLKSGTASVVCLLIVNFYPREAKVQWKVDNAL (SEQ ID NO:125) Anti-TIM-1 mAb 2.54 [02681 Nucleotide sequence of heavy chain variable region and a portion of constant region:
5'CAGGTGCAGCTGGAGCAGTCGGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCT
GAGACTCTCCTGTGCAGCGTCTGGATTCACCTTCACTAACTATGGCTTGCACTGG
GTCCGCCAGGCTCCAGGCAAGGGGCTGGATTGGGTGGCAGTTATATGGTATGAT
GGAAGTCATAAATTCTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGA
GACAATTCCAAGAACACGCTCTTTCTGCAAATGAACAGCCTGAGAGCCGAGGAC
ACGGCTGTGTATTACTGTACGCGAGATCTTGACTACTGGGGCCAGGGAACCCTG
-92-GTCACCGTCTCCTCAGCTTCCACCAAGGGCCCATCCGTCTTCCCCCTGGCGCCCT
GCTCCAGGAGCACCTCCGAGAGCACAGCCGCCCTGGGCTGCCTGGTCAAGGACT
ACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCG
TGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGC3' (SEQ
ID NO:25) 102691 Amino acid sequence of heavy chain variable region and a portion of constant region encoded by SEQ ID NO:25:
QVQLEQSGGGVVQPGRSLRLSCAASGFTFTNYGLHWV QAPGKGL ii,WVAVIWY
DGSHKFYADSVKGRF'TISRDNSKNTLFLQMNSLRAEDTAVYYCTRDLDYWGQG
TLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCL VKDYFPEPVTVSWNSGALTSGVHTFP
AVLQSSGLYSLS (SEQ ID NO:126) [0270] Nucleotide sequence of light chain variable region and a portion of constant region:
5' GAAACGCAGCTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAG
AGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAACAACTACTTAGCCTGG
TACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCA
GGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA
CTCTCACCATCAGCAGACTGGAGCCTGAAGATTGTGCAGAGTGTTACTGTCAGCA
ATATGGTAGCTCACTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAA
ACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTG
AAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGG
CCAAAGTACAGTGGGAAGGTGGGATAACGCCCTCCAATCGGGTA3' (SEQ ID
NO:27) [0271] Amino acid sequence of light chain variable region and a portion of constant region encoded by SEQ ID NO:27:
ETQLTQSPGTLSLSPGERVTLSCRASQSVSNNYLAWYQQKPGQAPRLLIYGASSR
ATGIPDRFSGSGSGTDFTLTISRLEPEDCAECYCQQYGSSLPLTFGGGTKVEIKRT
VAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWEGGITPSNRV (SEQ ID
NO:127) Anti-TIM-1 mAb 2.56 [0272] Nucleotide sequence of heavy chain variable region and a portion of constant region:
5' GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGG
GAGGTCCCTGAGACTCTCCTGTGCAGCGTCTGGATTCACCTTCAGTAGCTATGGC
-93-ATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATA
TGGTATGATGGAAGTCATAAATACTATGCAGACTCCGTGAAGGGCCGATTCACC
ATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGA
GCCGAGGACACGGCTGTGTATTACTCTGCGAGAGATTACTATGATACGAGTCGG
CATCACTGGGGGTTTGACTGCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCTG
CTTCCACCAAGGGCCCATCCGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTC
CGAGAGCACAGCCGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGT

' (SEQ ID NO:29) [0273] Amino acid sequence of heavy chain variable region and a portion of constant region encoded by SEQ ID NO: 29:
VOCQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVAV
IWYDGSHKY/LYA/TDSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYSARDYY
DTSRHHWGFDCWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEP
VTVSWNSGALTSGVHTFP (SEQ ID NO:128) [02741 Nucleotide sequence of light chain variable region and a portion of constant region:
5'CAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGAAATTGT
GATGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATC
TCCTGCAGGTCTAGTCAGAGCCTCTTGGATAGTGAAGATGGAAACACCTATTTGG
ACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTC
CCATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGA
TTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTGCTGC
ATGCAACGTGTAGAGTTTCCTATCACCTTCGGCCAAGGGACACGACTGGAGATT
AAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT
TGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGA
GGCCAAAGTACAGTGGAAGGTGGATAACGC3' (SEQ ID NO:31) [02751 Amino acid sequence of light chain variable region and a portion of constant region encoded by SEQ ID NO:31:
QLLGLLMLWVPGSSEEIVMTQTPLSLPVTPGEPASISCRSS S LLD SEDGNTYLDWY
LQKPGQSPQLLIYTLSHRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYCCMQR
VEFPITFGQGTRLEIKRTVAAPSYFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVD
N (SEQ ID NO:129)
-94-Anti-TIM-1 mAb 2.59 [0276] Nucleotide sequence of heavy chain variable region and a portion of constant region:
5' CAGTCGGGCCCAAGACTGGTGAAGCCTTCACAGACCCTGTCCCTCACCTGCAC
TGTCTCTGGTGGCTCCATCAGTAGTGATGGTTACTACTGGAGCTGGATCCGCCAG
CACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACC
TTCTACAACCCGTCCCTCAAGAGTCGAGTTGCCATATCAGTGGACACGTCTAAGA
ACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGC CGCGGACACGGCCGTGTATTA
CTGTGCGAGAGAATCCCCTCATAGCAGCAACTGGTACTCGGGCTTTGACTGCTGG
GGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTTCCACCAAGGGCCCATCCGTCT
TCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCCGCCCTGGGCT
GCCTGGTCAAGGACTACTTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGC
GCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT
CT3' (SEQ ID NO:33) [0277] Amino acid sequence of heavy chain variable region and a portion of constant region encoded by SEQ ID NO:33:
XXXXXQ SGPRLVI(PSQTLSLTCTVSGGSISSDGYYWSWIRQHPGKGLEWIGYIYY
SGSTFYNPSLKSRVAISVDTSKNQFSLKLSSVTAADTAVYYCARESPHSSNWYSGF
DCWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAAL GCLVKDYFPRTGDGVVELRRP
DQRRAHLPGCPTVLRTL (SEQ ID NO:130) [0278] Nucleotide sequence of light chain variable region and a portion of constant region:
'ACTCAGTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCAC
CTGCCGGGCCAGTCAGAGCATTGGTAGTAGGTTACACTGGTACCAGCAGAAACC
AGATCAGTCTCCAAAGCTCCTCATCAAGTATGCTTCCCAGTCCTTCTCAGGGGTC
CCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATA
GCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTAGTAATTTACC
ATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACC
ATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCT
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAG
GTGGATAACGCCCTC3' (SEQ ID NO:35) [0279] Amino acid sequence of light chain variable region and a portion of constant region encoded by SEQ ID NO:35:
XMTQSPDFQSVTPKEKVTITCRASQ SIGSRLHWYQQKPDQSPKLLIKYASQ SFS
GVPSRFSGSGSGTDFTLTINSLEAEDAATYYCHQ SSNLPFTFGPGTKVDIKRTVAAP
SVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNAL (SEQ ID NO:131)
-95-Anti-TIM-1 mAb 2.61 [0230] Nucleotide sequence of heavy chain variable region and a portion of constant region:
5' CAGGTGCAGCTGGTGGAGGCTGGGGGAGGCGTGGT C CAGC CTGGGAG GT C CCT
GAGACTCTCCTGTGCAGCGTCTGGATTCACCTTCAGAAGCTATGGCATGCACTGG
GTCCGCCAGGCTCCAGGCAAGGGGCTGAAATGGGTGGCAGTTATATGGTATGAT
GGAAGTAATAAATACTATACAGACTCCGTGAAGGGC C GATT CACCAT CTC CAGA
GACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGAC
ACGGCTGTGTATTACTGTGTGAGAGATTACTATGATAATAGTAGACATCACTGGG
GGTTTGACTACTGGGGCCAGGGAACCCTGGT CAC CGT CTCCT CAGCTTC CACCAA
GGGCCCATCCGTCTTCCCCCTGGCGCCCTGCTC CAGGAGCAC CT C CGAGAGCACA
GCCGCCCTGGG CTGCCTGGTCAAGGACTACTT C CCCGAACCGGT GACGGTGTCGT
GGAACTCAGGCGCCCTGACCAGGCGGCGTGCACACCTTCCCGGC3' (SEQ ID
NO:37) [0281] Amino acid sequence of heavy chain variable region and a portion of constant region encoded by SEQ ID NO:37:
QVQLVE/QAGGGVVQPGRSLRLSCAASGFTFRSYGMHWVRQAPGKGLKWVAVI
WYDGSNKY/LYTDSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCVRDYYDN
SRHHWGFDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVT
VSWNSGALTRRRAHLPG (SEQ ID NO:132) [02821 Nucleotide sequence of light chain variable region and a portion of constant region:
5' GACATCCAGATGACCCAGT CT C CAT CCTC CCGGTGTGCATC CGTAGGAGACAG
AGTCACCATCACTTGCCGGGCAAGTCAGGGCATCAGAAATGATTTAGCTTGGTAT
CAGCAGAAACCAGGGAAAGCCCCTAAGCGCCTGATCTATGCTGCATCCAGTTTG
CAAAGTGGGGT C CCATCAAGGTT CAGCGGCAGTAGATCTGGGACAGAATTCACT
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAGCTTATTACTGTCTCCAGC
ATAATAGTTACCCTCCCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA
CTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCT
GGAACTGCTAGCGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAA
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGG3 ' (SEQ ID NO:39) [0283] Amino acid sequence of light chain variable region and a portion of constant region encoded by SEQ ID NO:39:
-96-DIQMTQSPSSRCASVGDRVTITCRASQ GIRNDLAWYQQKPGKAPKRLIYAASSLQ
SGVPSRFSGSRSGTEFTLTISSLQPEDFAAYYCLOHNSYPPSFGQGTKLEIKRTVAA
PSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQS (SEQ ID NO:133) Anti-TIM-1 rriAb 2.70 [0284] Nucleotide sequence of heavy chain variable region and a portion of constant region:
5' CATGTGCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAG
GTCCCTGAGACTCTCCTGTGCAGCGTCTGGATTCATCTTCAGTCGCTATGGCATG
CACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGAAATGGGTGGCAGTTATATGG
TATGATGGAAGTAATAAACTCTATGCAGACTCCGTGAAGGGCCGATTCACCATCT
CCAGAGACAATTCCAAGAACACGCTGTAT CTGCAAATGAACAGCCTGAGAGCCG
AGGACACGGCTGTGTATTACTGTGCGAGAGATTACTATGATAATAGTAGACATC
ACTGGGGGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTTC
CACCAAGGGCCCATCCGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAG
AGCACAGCCGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACG
GTGTCGTGGAACTCAGGCGCCCTGA3' (SEQ ID NO:41) [0285] Amino acid sequence of heavy chain variable region and a portion of constant region encoded by SEQ ID NO:41:
HVQVQLVESGGGVVQPGRSLRLSCAASGFIFSRYGMHWVRQAPGKGLKWVAVI
WYDGSNKLYADSVKGRFTISRDNSICNTLYLQMNSLRAEDTAVYYCARDYYDNS
RHHWGFDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCL VKDYFPEPVTV
SWNSGAL (SEQ ID NO:134) [0286] Nucleotide sequence of light chain variable region and a portion of constant region:
5'TCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCAGTGAGGATATTGTG
ATGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCT
CCTGCAGGTCTAGTCGGAGCCTCTTGGATAGTGATGATGGAAACACCTATTTGGA
CTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTACACGCTTTCC
TATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGAT
TTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGC
ATGCAACGTGTAGAGTTTCCTATCACCTTCGGCCAAGGGACACGACTGGAGATT
AAACGAACTGTGGCTGCACCATCTGTCTICATCTTCCCGCCATCTGATGAGCAGT
TGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGA
GGCCAAAGTACAGTGGAAGGTGGATAACGCCT3' (SEQ ID NO:43)
-97-[0287] Amino acid sequence of light chain variable region and a portion of constant region encoded by SEQ ID NO:43:
SAPGAANALGP WISEDIVMTQTPLSLPVTP E PAS IS CRS S RS LLD SDDGNTYLDWIT

EFPITFGQGTRLEIKRTVAAPSVFIFPFSDEQLKSGTASVFICLLNNFITREAKTTWKVDN
A (SEQ ID NO:135) Anti-TIM-1 mAb 2.70.2 [0288] Nucleotide sequence of heavy chain variable region and a portion of constant region:
5' CGGCCGCCTATTTACCCAGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGT
GCAGAGCCTCATGCATCACGGAGCATGAGAAGACATTCCCCTCCTGCCACCTGCT
CTTGTCCACGGTTAGCCTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC
GGGAGGCGTGGTCTTGTAGTTGTT CT CC GGCTGC CCATTGCTCTCC CACTC CACG
GCGATGTCGCTGGGGTAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTC
TTGGTCATCTCCTCCTGGGATGGGGGCAGGGTGTACACCTGTGGCTCTCGGGGCT
GCCCTTTGGCTTTGGAGATGGTTTTCTCGAT GGAGGACGGGAGGCCTTTGTTGGA
GACCTTGCACTTGTACTCCTTGC CGTT CAGC CAGT C CTGGTGCAGGACGGTGAGG
ACGCTGACCACACGGTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGG
CATTATGCACCTCCACGCCATCCACGTACCAGTTGAACTGGACCTCGGGGTCTTC
CTGGCTCACGTCCACCACCACGCACGTGACCT CAGGGGTCCGGGAGATCATGAG
AGTGTCCTTGGGTTTTGGGGGGAACAGGAAGACTGATGGTCCCCCCAGGAACTC
AGGTGCTGGGCATGATGGGCATGGGGGACCATATTTGGACTCAACTCTCTTGTCC
ACCTTGGTGTTGCTGGGCTTGTGATCTACGTTGCAGGTGTAGGTCTTCGTGCCCA
AGCTGCTGGAGGGCACGGTCACCACGCTGCTGAGGGAGTAGAGTCCTGAGGACT
GTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC GCCTGAGTTCCACG
ACACCGTCACCGGTTCGGGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCGGCTG
TGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGGGGAAGACGGATGGGCCCT
TGGTGGAAGCTGAGGAGACGGTGACCAGGGTT CCCTGGCCCCAGTAGTCAAACC
CCCAGTGATGTCTACTATTATCATAGTAATCT CT CGCACAGTAATACACAGCCGT
GTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCGTGTT CTTGGAATTGTCTC
TGGAGATGGTGAATCGGCCCTTCACGGAGTCT GCATAGAGTTTATTACTTCCATC
ATACCATATAACTGCCACCCATTTCAGCCCCTTGCCTGGAGCCTGGCGGACCCAG
TGCATGCCATAGCGACTGAAGATGAATCCAGACGCTGCACAGGAGAGTCTCAGG
GACCTCCCAGGCTGGACCACGCCTCCCCCAGACTCCACCAGCTGCACCTGACACT
GGACACCTTTTAAAATAGCCACAAGAAAAAGCCAGCT CAGCCCAAACTCCATGG
TGGTCGACT3 ' (SEQ ID NO:136) [0289] Amino acid sequence of heavy chain variable region and a portion of constant region encoded by SEQ ID NO:136:
-98-MEFGLSWLFLVAILKGVQCQVQLVESGGGVVQPGRSLRLSCAASGFIFSRYGMHWV
RQAPGKGLKWVAVIWYDGSNKLYAD SVKGRFTISRDNSKNTLYLQMNSLRAED
TAVYYCARDYYDNSRHHWGFDYWGQGTLVTVSSASTICGPSVFPLAPCSRSTSESTA
ALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNV
DHICPSNTKVDKRVESKYGPPCPSCPAPEFLGGPSVFLFPPKPKDTLMISRTPET/TCVVVD
VSQEDPEVQFNIFYI/DGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVS
NKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNG
QPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSL
GK (SEQ ID NO:137) [0290] Nucleotide sequence of light chain variable region and a portion of constant region:
S'AGTCGACCACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGC
TCCCAGATACCACCGGAGATATTGTGATGACCCAGACTCCACTCTCCCTGCCCGT
CAC CC CTGGAGAGCCGGC CTCCATCTC CTGCAGGTCTAGTC GGAGCCT CTTGGAT
AGTGATGATGGAAACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCT
CCACAGCTCCTGATCTACACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGT
TCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGG
CTGAGGATGTTGGAGTTTATTACTGCATGCAACGTGTAGAGTTTCCTATCACCTT
CGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT
CATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC
CTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAAC
GCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC
AGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA
CACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACA
AAGAGCTTCAACAGGGGAGAGTGTTAGGCGGCCG3 (SEQ ID NO:138) [0291] Amino acid sequence of light chain variable region and portion constant region by SEQ ID NO:138:
METPAQLLFLLLLWLPDTTGDIVMTQTPLSLPVTPGEPASIS CRS SRS LLD SDD GNTY
LDWYLQI(PGQSPQLLIYTLSYRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYY
CMQRVEFPITFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQ
WKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKS
FNRGEC (SEQ ID NO:139) Anti-TIM-1 mAb 2.76 [0292] Nucleotide sequence of heavy chain variable region and a portion of constant region:
-99-5'GAGCAGTCGGGGGGCGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTG
TGCAGCGTCTGGATTCACCTTCAGTAGCTATGGCATGTACTGGGTCCGCCAGGCT
CCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGATGGAAGCAATAAA
TACTATGCAGACTCCGTGAAGGGCCGATTCAC CATCTCCAGAGACAATTCCAAG
AACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTAT
TACTGTGCGAGGGATTTCTATGATAGTAGTCGTTACCACTACGGTATGGACGTCT
GGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCTTCCACCAAGGGCCCATCCG
TCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCCGCCCTGGG
CTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGG
CGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTC
TCT3' (SEQ ID NO:45) [0293] Amino acid sequence of heavy chain variable region and a portion of constant region encoded by SEQ ID NO:45:
XXXXEQ SGGGVVQPGRSLRLSCAAS GFTF S S YGMYWVRQAPGKGLEWVAVIWY
D GSNKYYAD SVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARDFYD S SRYH
YGMDVWGQGTTVTVSSASTKGPSVFPLAPCSRSTSESTAAL GCLVKDYFPEPVTVSWN
SGALTSGVHTFPAVLQSSGLS (SEQ ID NO:140) [0294] Nucleotide sequence of light chain variable region and a portion of constant region:
S'ACTCAGTGTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCC
TGCAGGTCTAGTCAGAGCCTCTTGGATAGTGATGATGGAAACACCTATTTGGACT
GGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGGTTTCCTA
TCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTC
ACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATG
CAACGTATAGAGTTTCCGATCACCTTCGGCCAAGGGACCCGACTGGAGATTAAA
CGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA
AATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAA3' (SEQ ID NO:47) [0295] Amino acid sequence of light chain variable region and a portion of constant region encoded by SEQ lD NO:47:
XXXXTQCPLSLPVTPGEPASIS CRS S QSLLDSDDGNTYLDWYLQKPGQSPQLLIYT
VSYRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMORIEFPITFGQGTRLEI
KRTVAAPSVFIFPPSDEQLKSGTASVVCLLN (SEQ ID NO:141)
-100-[0296]
Equivalents [0297] While the preferred embodiment of the invention has been illustrated and described, it is to be understood that this invention is capable of variation and modification by those skilled in the art to which it pertains, and is therefore not limited to the precise terms set forth, but also such changes and alterations which may be made for adapting the invention to various usages and conditions. Accordingly, such changes and alterations are properly intended to be within the full range of equivalents, and therefore within the purview of the following claims.
[0298] The invention and the manner and a process of making and using it has been described in such full, clear, concise and exact terms so as to enable any person skilled in the art to which it pertains, or with which it is most nearly connected, to make and use the same.
-101-SEQUENCE LISTING
<110> Landes, Gregory M.
Chen, Francine Bezabeh, Binyam Foltz, Ian Tse, Kam Fai Jeffers, Michael Mesri, Mehdi Starling, Gary Mezes, Peter Khramtsov, Nikolia <120> ANTIBODIES AGAINST T CELL IMMUNOGLOBULIN
DOMAIN AND MUCIN DOMAIN 1 (TIM-1) ANTIGEN AND USES THEREOF
<130> 33651-0087 <140> CA 2,519,528 <141> 2004-03-19 <150> 60/456,652 <151> 2003-03-19 <160> 141 <170> FastSEQ for Windows Version 4.0 <210> 1 <211> 509 <212> DNA
<213> Homo Sapiens <400> 1 tgggtcctgt cccaggtgca gctgcaggag tcgggcccag gactggtgaa gccttcggag 60 accctgtccc tcacctgcac tgtctctggt ggctccgtca gcagtggtgg ttactactgg 120 agctggatcc ggcagccccc agggaaggga ctggagtgga ttgggtttat ctattacact 180 gggagcacca actacaaccc ctccctcaag agtcgagtct ccatatcagt agacacgtcc 240 aagaaccagt tctccctgaa gctgagctct gtgaccgctg cggacgcggc cgtgtattac 300 tgtgcgagag attatgactg gagcttccac tttgactact ggggccaggg aaccctggtc 360 accgtctcct cagcctccac caagggccca tcggtcttcc ccctggcgcc ctgctccagg 420 agcacctccg agagcacagc ggccctgggc tgcctggtca aggactactt ccccgaaccg 480 gtgacggtgt cgtggaactc aggcgctct 509 <210> 2 <211> 121 <212> PRT
<213> Homo Sapiens -104.1-<400> 2 Gin Val Gin Leu Gin Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Val Ser Ser Gly Gly Tyr Tyr Trp Ser Trp Ile Arg Gin Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly Phe Ile Tyr Tyr Thr Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys Ser Arg Val Ser Ile Ser Val Asp Thr Ser Lys Asn Gin Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Ala Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Asp Trp Ser Phe His Phe Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala <210> 3 <211> 504 <212> DNA
<213> Homo Sapiens <400> 3 cagctcctgg ggctcctgct gctctggttc ccaggtgcca ggtgtgacat ccagatgacc 60 cagtctccat cctccctgtc tgcatctata ggagacagag tcaccatcac ttgccgggca :20 agtcagggca ttagaaatga tttaggctgg tatcagcaga aaccagggaa agcccctaag 180 cgcctgatct atgctgcatc cagtttgcaa agtggggtcc catcaaggtt cagcggcagt 240 ggatctggga cagaattcac tctcacaatc agcagcctgc agcctgaaga ttttgcaact 300 tattactgtc tacagcataa tagttaccct ctcactttcg gcggagggac caaggtggag 360 atcaaacgaa ctgtggctgc accatctgtc ttcatcttcc cgccatctga tgagcagttg 420 aaatctggaa ctgcctctgt tgtgtgcctg ctgaataact tctatcccag agaggccaaa 480 gtacagtgga aggtggataa cgcc 504 <210> 4 <211> 108 <212> PRT
<213> Homo Sapiens <400> 4 Asp Ile Gin Met Thr Gin Ser Pro Ser Ser Leu Ser Ala Ser Ile Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gin Gly Ile Arg Asn Asp Leu Gly Trp Tyr Gln Gin Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile Tyr Ala Ala Ser Ser Leu Gin Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gin Pro -104.2-Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gin His Asn Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg <210> 5 <211> 469 <212> DNA
<213> Homo Sapiens <400> 5 cagtgtgagg tgcagctggt ggagtctggg ggaggcttgg tccagcctgg ggggtccctg 60 agactctcct gtgcagcctc tggattcacc tttactaact attggatgag ctgggtccgc 120 caggctccag ggaaggggct ggagtgggtg gccaacatac agcaagatgg aagtgagaaa 180 tactatgtgg actctgtgag gggccgattc accatctcca gagacaacgc caagaactca 240 ctgtatctgc aaatgaacag cctgagagcc gaggactcgg ctgtgtatta ctgtgcgaga 300 tgggactact ggggccaggg aaccctggtc accgtctcct cagcctccac caagggccca 360 tcggtcttcc ccctggcgcc ctgctccagg agcacctccg agagcacagc ggccctgggc 420 tgcctggtca aggactactt ccccgaaccg gtgagcggtg tcgtggaac 469 <210> 6 <211> 113 <212> PRT
<213> Homo Sapiens <400> 6 Glu Val Gin Leu Val Glu Ser Gly Gly Gly Leu Val Gin Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asn Tyr Trp Met Ser Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Asn Ile Gin Gin Asp Gly Ser Glu Lys Tyr Tyr Val Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Trp Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala <210> 7 <211> 454 <212> DNA
<213> Homo Sapiens -104.3-<400> 7 cttctggggc tgctaatgct ctgggtccct ggatccagtg gggatattgt gatgacccag 60 actccactct cctcaactgt catccttgga cagccggcct ccatctcctg caggtctagt 120 caaagcctcg tacacagtga tggaaacacc tacttgaatt ggcttcagca gaggccaggc 180 cagcctccaa gactcctaat ttatatgatt tctaaccggt tctctggggt cccagacaga 240 ttcagtggca gtggggcagg gacagatttc acactgaaaa tcagcagggt ggaagctgag 300 gatgtcgggg tttattactg catgcaagct acagaatctc ctcagacgtt cggccaaggg 360 accaaggtgg aaatcaaacg aactgtggct gcaccatctg tcttcatctt cccgccatct 420 gatgagcagt tgaaatctgg aagggcctct gttg 454 <210> 8 <211> 113 <212> PRT
<213> Homo Sapiens <400> 8 Asp Ile Val Met Thr Gin Thr Pro Leu Ser Ser Thr Val Ile Leu Gly Gin Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Val His Ser Asp Gly Asn Thr Tyr Leu Asn Trp Leu Gin Gin Arg Pro Gly Gin Pro Pro Arg Leu Leu Ile Tyr Met Ile Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ala Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Ala Thr Glu Ser Pro Gin Thr Phe Gly Gin Gly Thr Lys Val Glu Ile Lys Arg <210> 9 <211> 529 <212> DNA
<213> Homo Sapiens <400> 9 gagcagtcgg ggggaggcgt ggtaaagcct ggggggtctc ttagactctc ctgtgcagcc 60 tctggattca ctttcagtaa cgcctggatg acctgggtcc gccaggctcc agggaagggg 120 ctggagtggg ttggccgtat taaaaggaga actgatggtg ggacaacaga ctacgctgca 180 cccgtgaaag gcagattcac catctcaaga gatgattcaa aaaacacgct gtatctgcaa 240 atgaacaacc tgaaaaacga ggacacagcc gtgtattact gtacctcagt cgataatgac 300 gtggactact ggggccaggg aaccctggtc accgtctcct cagcttccac caagggccca 360 tccgtcttcc ccctggcgcc ctgctccagg agcacctccg agagcacagc cgccctgggc 420 tgcctggtca aggactactt ccccgaaccg gtgacggtgt cgtggaactc aggcgccctg 480 accagcggcg tgcacacctt cccggctgtc ctacagtcct caggactct 529 -104.4-<210> 10 <211> 119 <212> PRT
<213> Homo Sapiens <400> 10 Asn Asn Asn Asn Glu Gin Ser Gly Gly Gly Val Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Ala Trp Met Thr Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Glu Trp Val Gly Arg Ile Lys Arg Arg Thr Asp Gly Gly Thr Thr Asp Tyr Ala Ala Pro Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Leu Tyr Leu Gin Met Asn Asn Leu Lys Asn Glu Asp Thr Ala Val Tyr Tyr Cys Thr Ser Val Asp Asn Asp Val Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala <210> 11 <211> 447 <212> DNA
<213> Homo Sapiens <400> 11 ctgactcagt ctccactctc cctgcccgtc acccctggag agccggcctc catctcctgc 60 aggtctagtc agagcctcct gcatagtaat ggatacaact atttggattg gtacctgcag 120 aagccagggc agtctccaca gctcctgatc tatttgggtt ctaatcgggc ctccggggtc 180 cctgacaggt tcagtggcag tggatcaggc acagatttta cactgaaaat cagcagagtg 240 gaggctgagg atattggtct ttattactgc atgcaagctc tacaaactcc gctcactttc 300 ggcggaggga ccaaggtgga catcaaacga actgtggctg caccatctgt cttcatcttc 360 ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct gctgaataac 420 ttctatccca gagaggccaa agtacag 447 <210> 12 <211> 113 <212> PRT
<213> Homo Sapiens <400> 12 Asn Asn Asn Leu Thr Gin Ser Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Leu His Ser -104.5-Asn Gly Tyr Asn Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Leu Gly Ser Asn Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Ile Gly Leu Tyr Tyr Cys Met Gin Ala Leu Gin Thr Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Asp Ile Lys Arg <210> 13 <211> 588 <212> DNA
<213> Homo Sapiens <400> 13 caggtgcagc tggagcagtc ggggggaggc ttggtacagc ctggggggtc cctgagactc 60 tcctgtgcag cctctggatt caccttcagt acctatagca tgaactgggt ccgccaggct 120 ccagggaagg ggctggagtg ggtttcatac attagaagta gtactagtac catatactat 180 gcagagtccc tgaagggccg attcaccatc tccagcgaca atgccaagaa ttcactatat 240 ctgcaaatga acagcctgag agacgaggac acggctgtgt attactgtgc gcgggacttt 300 gactactggg gccagggaac cctggtcacc gtctcctcag cttccaccaa gggcccatcc 360 gtcttccccc tggcgccctg ctccaggagc acctccgaga gcacagccgc cctgggctgc 420 ctggtcaagg actacttccc cgaaccggtg acggtgtcgt ggaactcagg cgccctgacc 480 agcggcgtgc acaccttccc ggctgtccta cagtcctcag gactctactc cctcagca 538 <210> 14 <211> 114 <212> PRT
<213> Homo Sapiens <400> 14 Gin Val Gin Leu Glu Gin Ser Gly Gly Gly Leu Val Gin Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr Ser Met Asn Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Tyr Ile Arg Ser Ser Thr Ser Thr Ile Tyr Tyr Ala Glu Ser Leu Lys Gly Arg Phe Thr Ile Ser Ser Asp Asn Ala Lys Asn Ser Leu Tyr Leu Gin Met Asn Ser Leu Arg Asp Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Phe Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser -104.6-Ser Ala <210> 15 <211> 490 <212> DNA
<213> Homo Sapiens <400> 15 gaaatccagc tgactcagtc tccactctcc tcacctgtca cccttggaca gccggcctcc 60 atctcctgca ggtctagtca aagcctcgta cacagtgatg gagacaccta cttgaattgg 120 cttcagcaga ggccaggcca gcctccaaga ctcctaattt ataagatttc tacccggttc 180 tctggggtcc ctgacagatt cagtggcagt ggggcaggga cagatttcac actgaaaatc 240 agcagggtgg agactgacga tgtcgggatt tattactgca tgcaaactac acaaattcct 300 caaatcacct tcggccaagg gacacgactg gagattaaac gaactgtggc tgcaccatct 360 gtcttcatct tcccgccatc tgatgagcag ttgaaatctg gaactgcctc tgttgtgtgc 420 ctgctgaata acttctatcc cagagaggcc aaagtacagt ggaaggtgga taacgccctc 480 caatcgggta 490 <210> 16 <211> 114 <212> PRT
<213> Homo Sapiens <400> 16 Glu Ile Gin Leu Thr Gin Ser Pro Leu Ser Ser Pro Val Thr Leu Gly Gin Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Val His Ser Asp Gly Asp Thr Tyr Leu Asn Trp Leu Gin Gin Arg Pro Gly Gin Pro Pro Arg Leu Leu Ile Tyr Lys Ile Ser Thr Arg She Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ala Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Thr Asp Asp Val Gly Ile Tyr Tyr Cys Net Gin Thr Thr Gin Ile Pro Gin Ile Thr Phe Gly Gin Gly Thr Arg Leu Glu Ile Lys Arg <210> 17 <211> 568 <212> DNA
<213> Homo Sapiens <400> 17 -104.7-caggtgcagc tggagcagtc ggggggaggc gtggtccagc ctgggaggtc cctgagactc 60 tcctgtgcag cgtctggatt caccttcagt cgctatggca tgcactgggt ccgccaggct 120 ccaggcaagg ggctgaaatg ggtggcagtt atatggtatg atggaagtaa taaactctat 180 gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240 ctgcaaatga acagcctgag agccgaggac acggctgtgt attactgtgc gagagattac 300 tatgataata gtagacatca ctgggggttt gactactggg gccagggaac cctggtcacc 360 gtctcctcag cttccaccaa gggcccatcc gtcttccccc tggcgccctg ctccaggagc 420 acctccgaga gcacagccgc cctgggctgc ctggtcaagg actacttccc cgaaccggtg 480 acggtgtcgt ggaactcagg cgccctgacc agcggcgtgc acaccttccc ggctgtccta 540 cagtcctcag gactctactc cctcagca 568 <210> 18 <211> 124 <212> PRT
<213> Homo Sapiens <400> 18 Gin Val Gin Leu Glu Gin Ser Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Arg Tyr Gly Met His Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Lys Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Asp Asn Ser Arg His His Trp Gly Phe Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala <210> 19 <211> 472 <212> DNA
<213> Homo Sapiens <400> 19 gacatccagc tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc 60 atcacttgcc gggcaagtca gagtatttat agttatttaa attggtatca gcagaaacca 120 gggaaagccc ctaagctcct gatctatgct gcatccagtt tgcaaagtgg ggtcccatcc 180 aggttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct 240 gaagattttg caacttacta ctgtcaacag agttacagta cccctccgac gttcggccaa 300 gggaccaagg tggaaatcaa acgaactgtg gctgcaccat ctgtcttcat cttcccgcca 360 tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat 420 cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg ta 472 -104.8-<210> 20 <211> 108 <212> PRT
<213> Homo Sapiens <400> 20 Asp Ile Gin Leu Thr Gin Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gin Ser Ile Tyr Ser Tyr Leu Asn Trp Tyr Gin Gin Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gin Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gin Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gin Gin Ser Tyr Ser Thr Pro Pro Thr Phe Gly Gin Gly Thr Lys Val Glu Ile Lys Arg <210> 21 <211> 528 <212> DNA
<213> Homo Sapiens <400> 21 cagtcggggg gaggcttggt aaagcctggg gggtccctta gactctcctg tgcagcctct 60 ggattcactt tcagtaacgc ctggatgacc tgggtccgcc aggctccagg gaaggggctg 120 gagtgggttg gccgtattaa aaggaaaact gatggtggga caacagacta cgctgcaccc 180 gtgaaaggca gattcaccat ctcaagagat gattcagaaa acacgctgta tctgcaaatg 240 aacagcctgg aaaccgagga cacagccgtg tattactgta ccacagtcga taacagtggt 300 gactactggg gccagggaac cctggtcacc gtctcctcag cttccaccaa gggcccatcc 360 gtcttccccc tggcgccctg ctccaggagc acctccgaga gcacagccgc cctgggctgc 420 ctggtcaagg actacttccc cgaaccggtg acggtgtcgt ggaactcagg cgccctgacc 480 agcggcgtgc acaccttccc ggctgtccta cagtcctcag gactctct 528 <210> 22 <211> 119 <212> PRT
<213> Homo Sapiens <400> 22 Asn Asn Asn Asn Asn Gin Ser Gly Gly Gly Leu Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Ala Trp Met Thr Trp Val Arg Gin Ala Pro Gly Lys Gly Lou Glu Trp Val -104.9-Gly Arg Ile Lys Arg Lys Thr Asp Gly Gly Thr Thr Asp Tyr Ala Ala Pro Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Glu Asn Thr Leu Tyr Leu Gin Met Asn Ser Leu Glu Thr Glu Asp Thr Ala Val Tyr Tyr Cys Thr Thr Val Asp Asn Ser Gly Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala =
<210> 23 <211> 466 <212> DNA
<213> Homo Sapiens <400> 23 actcagtctc cactctccct gcccgtcacc cctggagagc cggcctccat ctcctgcagg 60 tctagtcaga gcctcctgca tagtaatgga tacaactatt tggattggta cctgcagaag 120 ccagggcagt ctccacagct cctgatctat ttgggttcta atcgggcctc cggggtccct 180 gacaggttca gtggcagtgg atcaggcaca gattttacac tgaaaatcag cagagtggag 240 gctgaggatg ttggggttta ttactgcatg caagctctac aaactccgct cactttcggc 300 ggagggacca aggtggagat caaacgaact gtggctgcac catctgtctt catcttcccg 360 ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc 420 tatcccagag aggccaaagt acagtggaag gtggataacg ccctca 466 <210> 24 <211> 113 <212> PRT
<213> Homo Sapiens <400> 24 Asn Asn Asn Asn Thr Gin Ser Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Leu His Ser Asn Gly Tyr Asn Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Leu Gly Ser Asn Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Ala Leu Gin Thr Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg -104.10-<210> 25 <211> 537 <212> DNA
<213> Homo Sapiens <400> 25 caggtgcagc tggagcagtc ggggggaggc gtggtccagc ctgggaggtc cctgagactc 60 tcctgtgcag cgtctggatt caccttcact aactatggct tgcactgggt ccgccaggct 120 ccaggcaagg ggctggattg ggtggcagtt atatggtatg atggaagtca taaattctat 180 gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctcttt 240 ctgcaaatga acagcctgag agccgaggac acggctgtgt attactgtac gcgagatctt 300 gactactggg gccagggaac cctggtcacc gtctcctcag cttccaccaa gggcccatcc 360 gtcttccccc tggcgccctg ctccaggagc acctccgaga gcacagccgc cctgggctgc 420 ctggtcaagg actacttccc cgaaccggtg acggtgtcgt ggaactcagg cgccctgacc 480 agcggcgtgc acaccttccc ggctgtccta cagtcctcag gactctactc cctcagc 537 <210> 26 <211> 114 <212> PRT
<213> Homo Sapiens <400> 26 Gin Val Gin Leu Glu Gin Ser Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asn Tyr Gly Leu His Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Asp Trp Val Ala Val Ile Trp Tyr Asp Gly Ser His Lys Phe Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Phe Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Thr Arg Asp Leu Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala <210> 27 <211> 480 <212> DNA
<213> Homo Sapiens <400> 27 gaaacgcagc tgacgcagtc tccaggcacc ctgtctttgt ctccagggga aagagtcacc 60 ctctcctgca gggccagtca gagtgttagc aacaactact tagcctggta ccagcagaaa 120 cctggccagg ctcccaggct cctcatctat ggtgcatcca gcagggccac tggcatccca 180 -104.11-gacaggttca gtggcagtgg gtctgggaca gacttcactc tcaccatcag cagactggag 240 cctgaagatt gtgcagagtg ttactgtcag caatatggta gctcactccc gctcactttc 300 ggcggaggga ccaaggtgga gatcaaacga actgtggctg caccatctgt cttcatcttc 360 ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct gctgaataac 420 ttctatccca gagaggccaa agtacagtgg gaaggtggga taacgccctc caatcgggta 480 <210> 28 <211> 110 <212> PRT
<213> Homo Sapiens <400> 28 Glu Thr Gln Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg Val Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Asn Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Lou Leu Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro Glu Asp Cys Ala Glu Cys Tyr Cys Gln Gln Tyr Gly Ser Ser Leu Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg <210> 29 <211> 542 <212> DNA
<213> Homo Sapiens <400> 29 gtccagtgtc aggtgcagct ggtggagtct gggggaggcg tggtccagcc tgggaggtcc 60 ctgagactct cctgtgcagc gtctggattc accttcagta gctatggcat gcactgggtc 120 dgccaggctc caggcaaggg gctggagtgg gtggcagtta tatggtatga tggaagtcat 180 aaatactatg cagactccgt gaagggccga ttcaccatct ccagagacaa ttccaagaac 240 acgctgtatc tgcaaatgaa cagcctgaga gccgaggaca cggctgtgta ttactctgcg 300 agagattact atgatacgag tcggcatcac tgggggtttg actgctgggg ccagggaacc 360 ctggtcaccg tctcctctgc ttccaccaag ggcccatccg tottocccct ggcgccctgc 420 tccaggagca cctccgagag cacagccgcc ctgggctgcc tggtcaagga ctacttcccc 480 gaaccggtga cggtgtcgtg gaactcaggc gccctgacca gcggcgtgca caccttcccg 540 gc 542 <210> 30 <211> 124 <212> PRT
<213> Homo Sapiens -104.12-<400> 30 Gin Val Gin Leu Val Glu Ser Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met His Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Val Ile Trp Tyr Asp Gly Ser His Lys Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser Ala Arg Asp Tyr Tyr Asp Thr Ser Arg His His Trp Gly Phe Asp Cys Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala <210> 31 <211> 521 <212> DNA
<213> Homo Sapiens <400> 31 cagctcctgg ggctgctaat gctctgggtc cctggatcca gtgaggaaat tgtgatgacc 60 cagactccac tctccctgcc cgtcacccct ggagagccgg cctccatctc ctgcaggtct 120 agtcagagcc tcttggatag tgaagatgga aacacctatt tggactggta cctgcagaag 180 ccagggcagt ctccacagct cctgatctat acgctttccc atcgggcctc tggagtccca 240 gacaggttca gtggcagtgg gtcaggcact gatttcacac tgaaaatcag cagggtggag 300 gctgaggatg ttggagttta ttgctgcatg caacgtgtag agtttcctat caccttcggc 360 caagggacac gactggagat taaacgaact gtggctgcac catctgtctt catcttcccg 420 ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc 480 tatcccagag aggccaaagt acagtggaag gtggataacg c 521 <210> 32 <211> 114 <212> PRT
<213> Homo Sapiens <400> 32 Glu Ile Val Met Thr Gin Thr Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Leu Asp Ser Glu Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Thr Leu Ser His Arg Ala Ser Gly Val -104.13-Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Cys Cys Met Gin Arg Val Glu Phe Pro Ile Thr Phe Gly Gin Gly Thr Arg Leu Glu Ile Lys Arg <210> 33 <211> 547 <212> DNA
<213> Homo Sapiens <400> 33 cagtcgggcc caagactggt gaagccttca cagaccctgt ccctcacctg cactgtctct 60 ggtggctcca tcagtagtga tggttactac tggagctgga tccgccagca cccagggaag 120 ggcctggagt ggattgggta catctattac agtgggagca ccttctacaa cccgtccctc 180 aagagtcgag ttgccatatc agtggacacg tctaagaacc agttctccct gaagctgagc 240 tctgtgactg ccgcggacac ggccgtgtat tactgtgcga gagaatcccc tcatagcagc 300 aactggtact cgggctttga ctgctggggc cagggaaccc tggtcaccgt ctcctcagct 360 tccaccaagg gcccatccgt cttccccctg gcgccctgct ccaggagcac ctccgagagc 420 acagccgccc tgggctgcct ggtcaaggac tactttcccc gaaccggtga cggtgtcgtg 480 gaactcaggc gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg 540 actctct 547 <210> 34 <211> 125 <212> PRT
<213> Homo Sapiens <400> 34 Asn Asn Asn Asn Asn Gin Ser Gly Pro Arg Leu Val Lys Pro Ser Gin Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser Asp Gly Tyr Tyr Trp Ser Trp Ile Arg Gin His Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Phe Tyr Asn Pro Ser Leu Lys Ser Arg Val Ala Ile Ser Val Asp Thr Ser Lys Asn Gin Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu Ser Pro His Ser Ser Asn Trp Tyr Ser Gly Phe Asp Cys Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala -104.14-<210> 35 <211> 450 <212> DNA
<213> Homo Sapiens <400> 35 actcagtctc cagactttca gtctgtgact ccaaaggaga aagtcaccat cacctgccgg 60 gccagtcaga gcattggtag taggttacac tggtaccagc agaaaccaga tcagtctcca 120 aagctcctca tcaagtatgc ttcccagtcc ttctcagggg tcccctcgag gttcagtggc 180 agtggatctg ggacagattt caccctcacc atcaatagcc tggaagctga agatgctgca 240 acgtattact gtcatcagag tagtaattta ccattcactt tcggccctgg gaccaaagtg 300 gatatcaaac gaactgtggc tgcaccatct gtcttcatct tcccgccatc tgatgagcag 360 ttgaaatctg gaactgcctc tgttgtgtgc ctgctgaata acttctatcc cagagaggcc 420 aaagtacagt ggaaggtgga taacgccctc 450 <210> 36 <211> 108 <212> PRT
<213> Homo Sapiens <400> 36 Asn Asn Asn Asn Thr Gin Ser Pro Asp Phe Gin Ser Val Thr Pro Lys Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gin Ser Ile Gly Ser Arg Leu His Trp Tyr Gin Gin Lys Pro Asp Gin Ser Pro Lys Leu Leu Ile Lys Tyr Ala Ser Gin Ser Phe Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys His Gin Ser Ser Asn Leu Pro Phe Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys Arg <210> 37 <211> 534 <212> DNA
<213> Homo Sapiens <400> 37 caggtgcagc tggtggaggc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60 tcctgtgcag cgtctggatt caccttcaga agctatggca tgcactgggt ccgccaggct 120 ccaggcaagg ggctgaaatg ggtggcagtt atatggtatg atggaagtaa taaatactat 180 acagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240 ctgcaaatga acagcctgag agccgaggac acggctgtgt attactgtgt gagagattac 300 tatgataata gtagacatca ctgggggttt gactactggg gccagggaac cctggtcacc 360 -104.15-gtctcctcag cttccaccaa gggcccatcc gtcttccccc tggcgccctg ctccaggagc 420 acctccgaga gcacagccgc cctgggctgc ctggtcaagg actacttccc cgaaccggtg 480 acggtgtcgt ggaactcagg cgccctgacc aggcggcgtg cacaccttcc cggc 534 <210> 38 <211> 124 <212> PRT
<213> Homo Sapiens <400> 38 Gin Val Gin Leu Val Glu Ala Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Arg Ser Tyr Gly Met His Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Lys Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Tyr Tyr Thr Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg Asp Tyr Tyr Asp Asn Ser Arg His His Trp Gly Phe Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala <210> 39 <211> 470 <212> DNA
<213> Homo Sapiens <400> 39 gacatccaga tgacccagtc tccatcctcc cggtgtgcat ccgtaggaga cagagtcacc 60 atcacttgcc gggcaagtca gggcatcaga aatgatttag cttggtatca gcagaaacca 120 gggaaagccc ctaagcgcct gatctatgct gcatccagtt tgcaaagtgg ggtcccatca 180 aggttcagcg gcagtagatc tgggacagaa ttcactctca caatcagcag cctgcagcct 240 gaagattttg cagcttatta ctgtctccag cataatagtt accctcccag ttttggccag 300 gggaccaagc tggagatcaa acgaactgtg gctgcaccat ctgtcttcat cttcccgcca 360 tctgatgagc agttgaaatc tggaactgct agcgttgtgt gcctgctgaa taacttctat 420 cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg 470 <210> 40 <211> 108 <212> PRT
<213> Homo Sapiens <400> 40 Asp Ile Gln Met Thr Gin Ser Pro Ser Ser Arg Cys Ala Ser Val Gly -104.16-Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gin Gly Ile Arg Asn Asp Leu Ala Trp Tyr Gin Gin Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile Tyr Ala Ala Ser Ser Leu Gin Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gin Pro Glu Asp Phe Ala Ala Tyr Tyr Cys Leu Gin His Asn Ser Tyr Pro Pro Ser Phe Gly Gin Gly Thr Lys Leu Glu Ile Lys Arg <210> 41 <211> 514 <212> DNA
<213> Homo Sapiens <400> 41 catgtgcagg tgcagctggt ggagtctggg ggaggcgtgg tccagcctgg gaggtccctg 60 agactctcct gtgcagcgtc tggattcatc ttcagtcgct atggcatgca ctgggtccgc 120 caggctccag gcaaggggct gaaatgggtg gcagttatat ggtatgatgg aagtaataaa 180 ctctatgcag actccgtgaa gggccgattc accatctcca gagacaattc caagaacacg 240 ctgtatctgc aaatgaacag cctgagagcc gaggacacgg ctgtgtatta ctgtgcgaga 300 gattactatg ataatagtag acatcactgg gggtttgact actggggcca gggaaccctg 360 gtcaccgtct cctcagcttc caccaagggc ccatccgtct tccccctggc gccctgctcc 420 aggagcacct ccgagagcac agccgccctg ggctgcctgg tcaaggacta cttccccgaa 480 ccggtgacgg tgtcgtggaa ctcaggcgcc ctga 514 <210> 42 <211> 124 <212> PRT
<213> Homo Sapiens <400> 42 Gin Val Gin Leu Val Glu Ser Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ile Phe Ser Arg Tyr Gly Met His Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Lys Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Asp Asn Ser Arg His His Trp Gly Phe Asp Tyr -104.17-100 ' 105 110 Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala <210> 43 <211> 523 <212> DNA
<213> Homo Sapiens <400> 43 tcagctcctg gggctgctaa tgctctgggt ccctggatca gtgaggatat tgtgatgacc 60 cagactccac tctccctgcc cgtcacccct ggagagccgg cctccatctc ctgcaggtct 120 agtcggagcc tcttggatag tgatgatgga aacacctatt tggactggta cctgcagaag 180 ccagggcagt ctccacagct cctgatctac acgctttcct atcgggcctc tggagtccca 240 gacaggttca gtggcagtgg gtcaggcact gatttcacac tgaaaatcag cagggtggag 300 gctgaggatg ttggagttta ttactgcatg caacgtgtag agtttcctat caccttcggc 360 caagggacac gactggagat taaacgaact gtggctgcac catctgtctt catcttcccg 420 ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc 480 tatcccagag aggccaaagt acagtggaag gtggataacg cct 523 <210> 44 <211> 114 <212> PRT
<213> Homo Sapiens <400> 44 Asp Ile Val Met Thr Gin Thr Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Arg Ser Leu Leu Asp Ser Asp Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Thr Leu Ser Tyr Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Arg Val Glu Phe Pro Ile Thr Phe Gly Gin Gly Thr Arg Leu Glu Ile Lys Arg <210> 45 <211> 546 <212> DNA
<213> Homo Sapiens -104.18-<400> 45 gagcagtcgg ggggcggcgt ggtccagcct gggaggtccc tgagactctc ctgtgcagcg 60 tctggattca ccttcagtag ctatggcatg tactgggtcc gccaggctcc aggcaagggg 120 ctggagtggg tggcagttat atggtatgat ggaagcaata aatactatgc agactccgtg 180 aagggccgat tcaccatctc cagagacaat tccaagaaca cgctgtatct gcaaatgaac 240 agcctgagag ccgaggacac ggctgtgtat tactgtgcga gggatttcta tgatagtagt 300 cgttaccact acggtatgga cgtctggggc caagggacca cggtcaccgt ctcctcagct 360 tccaccaagg gcccatccgt cttccccctg gcgccotgct ccaggagcac ctccgagagc 420 acagccgccc tgggctgcct ggtcaaggac tacttccccg aaccggtgac ggtgtcgtgg 480 aactcaggcg ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga 540 ctctct 546 <210> 46 <211> 124 <212> PRT
<213> Homo Sapiens <400> 46 Asn Asn Asn Asn Glu Gin Ser Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met Tyr Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Phe Tyr Asp Ser Ser Arg Tyr His Tyr Gly Met Asp Val Trp Gly Gin Gly Thr Thr Val Thr Val Ser Ser Ala <210> 47 <211> 419 <212> DNA
<213> Homo Sapiens <400> 47 actcagtgtc cactctccct gcccgtcacc cctggagagc cggcctccat ctcctgcagg 60 tctagtcaga gcctcttgga tagtgatgat ggaaacacct atttggactg gtacctgcag 120 aagccagggc agtctccaca gctcctgatc tatacggttt cctatcgggc ctctggagtc 180 ccagacaggt tcagtggcag tgggtcaggc actgatttca cactgaaaat cagcagggtg 240 gaggctgagg atgttggagt ttattactgc atgcaacgta tagagtttcc gatcaccttc 300 ggccaaggga cccgactgga gattaaacga actgtggctg caccatctgt cttcatcttc 360 ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct gctgaataa 419 -104.19-<210> 48 <211> 114 <212> PRT
<213> Homo Sapiens <400> 48 Asn Asn Asn Asn Thr Gin Cys Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Leu Asp Ser Asp Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Thr Val Ser Tyr Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Arg Ile Glu Phe Pro Ile Thr Phe Gly Gin Gly Thr Arg Leu Glu Ile Lys Arg <210> 49 <211> 789 <212> DNA
<213> Homo Sapiens <400> 49 tctgtaaagg ttggtggaga ggcaggtcca tctgtcacac taccctgcca ctacagtgga 60 gctgtcacat caatgtgctg gaatagaggc tcatgttctc tattcacatg ccaaaatggc :20 attgtctgga ccaatggaac ccacgtcacc tatcggaagg acacacgcta taagctattg 180 ggggaccttt caagaaggga tgtctctttg accatagaaa atacagctgt gtctgacagt 240 ggcgtatatt gttgccgtgt tgagcaccgt gggtggttca atgacatgaa aatcaccgta 300 tcattggaga ttgtgccacc caaggtcacg actactccaa ttgtcacaac tgttccaacc 360 gtcacgactg ttcgaacgag caccactgtt ccaacgacaa cgactgttcc aacgacaact 420 gttccaacaa caatgagcat tccaacgaca acgactgttc cgacgacaat gactgtttca 480 acgacaacga gcgttccaac gacaacgagc attccaacaa caacaagtgt tccagtgaca 540 acaacggtct ctacctttgt tcctccaatg cctttgccca ggcagaacca tgaaccagta 600 gccacttcac catcttcacc tcagccagca gaaacccacc ctacgacact gcagggagca 660 ataaggagag aacccaccag ctcaccattg tactcttaca caacagatgg gaatgacacc 720 gtgacagagt cttcagatgg cctttggaat aacaatcaaa ctcaactgtt cctagaacat 780 agtctactg 789 <210> 50 <211> 263 <212> PRT
<213> Homo Sapiens -104.20-<400> 50 Ser Val Lys Val Gly Gly Glu Ala Gly Pro Ser Val Thr Leu Pro Cys His Tyr Ser Gly Ala Val Thr Ser Met Cys Trp Asn Arg Gly Ser Cys Ser Leu Phe Thr Cys Gln Asn Gly Ile Val Trp Thr Asn Gly Thr His Val Thr Tyr Arg Lys Asp Thr Arg Tyr Lys Leu Leu Gly Asp Leu Ser Arg Arg Asp Val Ser Leu Thr Ile Glu Asn Thr Ala Val Ser Asp Ser Gly Val Tyr Cys Cys Arg Val Glu His Arg Gly Trp Phe Asn Asp Met Lys Ile Thr Val Ser Leu Glu Ile Val Pro Pro Lys Val Thr Thr Thr Pro Ile Val Thr Thr Val Pro Thr Val Thr Thr Val Arg Thr Ser Thr Thr Val Pro Thr Thr Thr Thr Val Pro Thr Thr Thr Val Pro Thr Thr Met Ser Ile Pro Thr Thr Thr Thr Val Pro Thr Thr Met Thr Val Ser Thr Thr Thr Ser Val Pro Thr Thr Thr Ser Ile Pro Thr Thr Thr Ser Val Pro Val Thr Thr Thr Val Ser Thr Phe Val Pro Pro Met Pro Leu Pro Arg Gin Asn His Glu Pro Val Ala Thr Ser Pro Ser Ser Pro Gin Pro Ala Glu Thr His Pro Thr Thr Leu Gin Gly Ala Ile Arg Arg Glu Pro Thr Ser Ser Pro Leu Tyr Ser Tyr Thr Thr Asp Gly Asn Asp Thr Val Thr Glu Ser Ser Asp Gly Leu Trp Asn Asn Asn Gin Thr Gin Leu Phe Leu Glu His Ser Leu Leu <210> 51 <211> 114 <212> PRT
<213> Homo Sapiens <400> 51 Gin Val Gin Leu Val Glu Ser Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met His Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val -104.21-Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Asn Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala <210> 52 <211> 124 <212> PRT
<213> Homo Sapiens <400> 52 Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Asn Asn Asn Tyr Asp Ser Ser Asn Asn Asn Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala <210> 53 <211> 125 <212> PRT
<213> Homo Sapiens <400> 53 Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser Gly Gly Tyr Tyr Trp Ser Trp Ile Arg Gln His Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Tyr Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe -104.22-Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Asn Asn Asn Ser Ser Ser Trp Tyr Asn Asn Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala <210> 54 <211> 124 <212> PRT
<213> Homo Sapiens <400> 54 Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Asp Ser Ser Asn Asn Asn Asn Asn Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala <210> 55 <211> 119 <212> PRT
<213> Homo Sapiens <400> 55 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Ala Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Gly Arg Ile Lys Ser Lys Thr Asp Gly Gly Thr Thr Asp Tyr Ala Ala Pro Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr -104.23-Tyr Cys Thr Asn Asn Asp Asn Asn Asn Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala <210> 56 <211> 121 <212> PRT
<213> Homo Sapiens <400> 56 Gin Val Gin Leu Gin Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Val Ser Ser Gly Gly Tyr Tyr Trp Ser Trp Ile Arg Gin Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gin Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Asn Asn Trp Asn Asn Asn Phe Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala <210> 57 <211> 119 <212> PRT
<213> Homo Sapiens <400> 57 Glu Val Gin Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Ala Trp Met Ser Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Glu Trp Val Gly Arg Ile Lys Ser Lys Thr Asp Gly Gly Thr Thr Asp Tyr Ala Ala Pro Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Leu Tyr Leu Gin Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys Thr Thr Asn Asn Asn Ser Gly Asp Tyr Trp Gly Gin Gly Thr -104.24-Leu Val Thr Val Ser Ser Ala <210> 58 <211> 113 <212> PRT
<213> Homo Sapiens <400> 58 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Asn Ile Lys Gln Asp Gly Ser Glu Lys Tyr Tyr Val Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala <210> 59 <211> 114 <212> PRT
<213> Homo Sapiens <400> 59 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Ser Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Tyr Ile Ser Ser Ser Ser Ser Thr Ile Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Asp Glu Asp Thr Ala Val Tyr Tyr Cys Ala Asn Asn Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala -104.25-<210> 60 <211> 110 <212> PRT
<213> Homo Sapiens <400> 60 Glu Ile Val Leu Thr Gin Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gin Ser Val Ser Ser Ser Tyr Leu Ala Trp Tyr Gin Gin Lys Pro Gly Gin Ala Pro Arg Leu Leu Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gin Gin Tyr Gly Ser Ser Asn Asn Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg <210> 61 <211> 113 <212> PRT
<213> Homo Sapiens <400> 61 Asp Ile Val Met Thr Gin Ser Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Leu His Ser 20 25 .30 Asn Gly Tyr Asn Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Leu Gly Ser Asn Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Ala Leu Gin Thr Asn Asn Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg <210> 62 -104.26-<211> 108 <212> PRT
<213> Homo Sapiens <400> 62 Asp Ile Gin Met Thr Gin Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gin Gly Ile Arg Asn Asp Leu Gly Trp Tyr Gin Gin Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile Tyr Ala Ala Ser Ser Leu Gin Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gin Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gin His Asn Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg <210> 63 <211> 114 <212> PRT
<213> Homo Sapiens <400> 63 Asp Ile Val Met Thr Gin Thr Pro Leu Ser Ser Pro Val Thr Leu Gly Gin Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Val His Ser Asp Gly Asn Thr Tyr Leu Ser Trp Leu Gin Gin Arg Pro Gly Gin Pro 35 40 45 .
Pro Arg Leu Leu Ile Tyr Lys Ile Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ala Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Ala Thr Gin Phe Pro Asn Ile Thr Phe Gly Gin Gly Thr Arg Leu Glu Ile Lys Arg <210> 64 <211> 108 <212> PRT
<213> Homo Sapiens -104.27-<400> 64 Asp Ile Gin Met Thr Gin Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gin Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gin Gin Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gin Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gin Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gin Gin Ser Tyr Ser Thr Pro Pro Thr Phe Gly Gin Gly Thr Lys Val Glu Ile Lys Arg <210> 65 <211> 113 <212> PRT
<213> Homo Sapiens <400> 65 Asp Ile Val Met Thr Gin Thr Pro Leu Ser Ser Pro Val Thr Leu Gly Gin Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Val His Ser Asp Gly Asn Thr Tyr Leu Ser Trp Leu Gin Gin Arg Pro Gly Gin Pro Pro Arg Leu Leu Ile Tyr Lys Ile Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ala Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Ala Thr Gin Phe Pro Gin Thr Phe Gly Gin Gly Thr Lys Val Glu Ile Lys Arg <210> 66 <211> 114 <212> PRT
<213> Homo Sapiens <400> 66 Asp Ile Val Met Thr Gin Thr Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Leu Asp Ser -104.28-Asp Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Thr Leu Ser Tyr Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Arg Ile Glu Phe Pro Ile Thr Phe Gly Gin Gly Thr Arg Leu Glu Ile Lys Arg <210> 67 <211> 108 <212> PRT
<213> Homo Sapiens <400> 67 Glu Ile Val Leu Thr Gin Ser Pro Asp Phe Gin Ser Val Thr Pro Lys Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gin Ser Ile Gly Ser Ser Leu His Trp Tyr Gin Gin Lys Pro Asp Gin Ser Pro Lys Leu Leu Ile Lys Tyr Ala Ser Gin Ser Phe Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys His Gin Ser Ser Ser Leu Pro Phe Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys Arg <210> 68 <211> 108 <212> PRT
<213> Homo Sapiens <400> 68 Asp Ile Gin Met Thr Gin Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gin Gly Ile Arg Asn Asp Leu Gly Trp Tyr Gin Gin Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile Tyr Ala Ala Ser Ser Leu Gin Ser Gly Val Pro Ser Arg Phe Ser Gly -104.29-Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gin Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gin His Asn Ser Tyr Pro Asn Asn Phe Gly Gin Gly Thr Lys Leu Glu Ile Lys Arg <210> 69 <211> 113 <212> PRT
<213> Homo Sapiens <400> 69 Asp Ile Val Met Thr Gin Thr Pro Leu Ser Ser Pro Val Thr Leu Gly Gin Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Val His Ser Asp Gly Asn Thr Tyr Leu Ser Trp Leu Gin Gin Arg Pro Gly Gin Pro Pro Arg Leu Leu Ile Tyr Lys Ile Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ala Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Ala Thr Gin Phe Pro Gin Thr Phe Gly Gin Gly Thr Lys Val Glu Ile Lys Arg <210> 70 <211> 114 <212> PRT
<213> Homo Sapiens <400> 70 Asp Ile Val Met Thr Gin Thr Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Leu Asp Ser Asp Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Thr Leu Ser Tyr Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin -104.30-Arg Ile Glu Phe Pro Ile Thr Phe Gly Gin Gly Thr Arg Leu Glu Ile Lys Arg <210> 71 <211> 108 <212> PRT
<213> Homo Sapiens <400> 71 Glu Ile Val Leu Thr Gin Ser Pro Asp Phe Gin Ser Val Thr Pro Lys Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gin Ser Ile Gly Ser Ser Leu His Trp Tyr Gin Gin Lys Pro Asp Gin Ser Pro Lys Leu Leu Ile Lys Tyr Ala Ser Gin Ser Phe Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys His Gin Ser Ser Ser Leu Pro Phe Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys Arg <210> 72 <211> 108 <212> PRT
<213> Homo Sapiens <220>
<223> Xaa = any amino acid <400> 72 Asp Ile Gin Met Thr Gin Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gin Gly Ile Arg Asn Asp Leu Gly Trp Tyr Gin Gin Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile Tyr Ala Ala Ser Ser Leu Gin Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gin Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gin His Asn Ser Tyr Pro Xaa -104.31-Xaa Phe Gly Gin Gly Thr Lys Leu Glu Ile Lys Arg <210> 73 <211> 16 <212> DNA
<213> Homo Sapiens <400> 73 ttactatgat aatagt 16 <210> 74 <211> 15 <212> DNA
<213> Homo Sapiens <400> 74 agacatcact ggggg 15 <210> 75 <211> 17 <212> DNA
<213> Homo Sapiens <400> 75 atagcagcaa ctggtac 17 <210> 76 <211> 16 <212> DNA
<213> Homo Sapiens <400> 76 ttactatgat aatagt 16 <210> 77 <211> 15 <212> DNA
<213> Homo Sapiens <400> 77 agacatcact ggggg 15 <210> 78 <211> 16 <212> DNA
<213> Homo Sapiens <400> 78 -104.32-ttactatgat aatagt <210> 79 <211> 15 <212> DNA
<213> Homo Sapiens <400> 79 agacatcact ggggg 15 <210> 80 <211> 13 <212> DNA
<213> Homo Sapiens <400> 80 ctatgatagt agt 13 <210> 81 <211> 11 <212> DNA
<213> Homo Sapiens <400> 81 ttactatgat a 11 <210> 82 <211> 20 <212> DNA
<213> Homo Sapiens <400> 82 cgagtcggca tcactggggg 20 <210> 83 <211> 22 <212> DNA
<213> Homo Sapiens <400> 83 caggtgcagc tggagcagtc gg 22 <210> 84 <211> 24 <212> DNA
<213> Homo Sapiens <400> 84 gctgagggag tagagtcctg agga 24 -104.33-<210> 85 <211> 19 <212> DNA
<213> Homo Sapiens <400> 85 cacaccgcgg tcacatggc 19 <210> 86 <211> 20 <212> DNA
<213> Homo Sapiens <400> 86 ctactctagg gcacctgtcc 20 <210> 87 <211> 14 <212> PRT
<213> Homo Sapiens <400> 87 Pro Met Pro Leu Pro Arg Gin Asn His Glu Pro Val Ala Thr <210> 88 <211> 12 <212> PRT
<213> Homo Sapiens <400> 88 Pro Met Pro Leu Pro Arg Gin Asn His Glu Pro Val <210> 89 <211> 10 <212> PRT
<213> Homo Sapiens <400> 89 Pro Met Pro Leu Pro Arg Gin Asn His Glu <210> 90 <211> 8 <212> PRT
<213> Homo Sapiens -104.34-<400> 90 Pro Met Pro Leu Pro Arg Gin Asn <210> 91 <211> 6 <212> PRT
<213> Homo Sapiens <400> 91 Pro Met Pro Leu Pro Arg <210> 92 <211> 12 <212> PRT
<213> Homo Sapiens <400> 92 Pro Leu Pro Arg Gin Asn His Glu Pro Val Ala Thr <210> 93 <211> 10 <212> PRT
<213> Homo Sapiens <400> 93 Pro Arg Gin Asn His Glu Pro Val Ala Thr <210> 94 <211> 8 <212> PRT
<213> Homo Sapiens <400> 94 Gin Asn His Glu Pro Val Ala Thr <210> 95 <211> 6 <212> PRT
<213> Homo Sapiens -104.35-<400> 95 His Glu Pro Val Ala Thr <210> 96 <211> 7 <212> PRT
<213> Homo Sapiens <400> 96 Pro Leu Pro Arg Asn His Glu <210> 97 <211> 6 <212> PRT
<213> Homo Sapiens <400> 97 Leu Pro Arg Gin Asn His <210> 98 <211> 10 <212> PRT
<213> Homo Sapiens <400> 98 Pro Met Pro Ala Pro Arg Gin Asn His Glu <210> 99 <211> 10 <212> PRT
<213> Homo Sapiens <400> 99 Pro Met Pro Leu Ala Arg Gin Asn His Glu <210> 100 <211> 10 <212> PRT
<213> Homo Sapiens -104.36-<400> 100 Pro Met Pro Leu Pro Ala Gin Asn His Glu <210> 101 <211> 10 <212> PRT
<213> Homo Sapiens <400> 101 Pro Met Pro Leu Pro Arg Ala Asn His Glu <210> 102 <211> 10 <212> PRT
<213> Homo Sapiens <400> 102 Pro Met Pro Leu Pro Arg Gin Ala His Glu <210> 103 <211> 10 <212> PRT
<213> Homo Sapiens <400> 103 Pro Met Pro Leu Pro Arg Gin Asn Ala Glu <210> 104 <211> 8 <212> PRT
<213> Homo Sapiens <400> 104 Pro Leu Pro Arg Gin Asn His Glu <210> 105 <211> 7 <212> PRT
<213> Homo Sapiens -104.37-<400> 105 Leu Pro Arg Gin Asn His Glu <210> 106 <211> 8 <212> PRT
<213> Homo Sapiens <400> 106 Pro Leu Pro Arg Gin Asn His Glu <210> 107 <211> 7 <212> PRT
<213> Homo Sapiens <400> 107 Leu Pro Arg Gin Asn His Glu <210> 108 <211> 882 <212> DNA
<213> Homo Sapiens <400> 108 atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcc 60 atggccgata ttgtgatgac ccagactcca ctctccctgc ccgtcacccc tggagagccg 120 gcctccatct cctgcaggtc tagtcggagc ctcttggata gtgatgatgg aaacacctat 180 ttggactggt acctgcagaa gccagggcag tctccacagc tcctgatcta cacgctttcc 240 tatcgggcct ctggagtccc agacaggttc agtggcagtg ggtcaggcac tgatttcaca 300 ctgaaaatca gcagggtgga ggctgaggat gttggagttt attactgcat gcaacgtgta 360 gagtttccta tcaccttcgg ccaagggaca cgactggaga ttaaactttc cgcggacgat 420 gcgaaaaagg atgctgcgaa gaaagatgac gctaagaaag acgatgctaa aaaggacctc 480 caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 540 tcctgtgcag cgtctggatt catcttcagt cgctatggca tgcactgggt ccgccaggct 600 ccaggcaagg ggctgaaatg ggtggcagtt atatggtatg atggaagtaa taaactctat 660 gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 720 ctgcaaatga acagcctgag agccgaggac acggctgtgt attactgtgc gagagattac 780 tatgataata gtagacatca ctgggggttt gactactggg gccagggaac cctggtcacc 840 gtctcctcag ctagcgatta taaggacgat gatgacaaat ag 882 <210> 109 <211> 271 -104.38-<212> PRT
<213> Homo Sapiens <400> 109 Asp Ile Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Arg Ser Leu Leu Asp Ser Asp Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Thr Leu Ser Tyr Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Arg Val Glu Phe Pro Ile Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys Leu Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala Lys Lys Asp Asp Ala Lys Lys Asp Asp Ala Lys Lys Asp Leu Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ile Phe Ser Arg Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Lys Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Asp Asn Ser Arg His His Trp Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Asp Tyr Lys Asp Asp Asp Asp Lys <210> 110 <211> 1560 <212> DNA
<213> Homo Sapiens <400> 110 atggaaaccc cagcgcagct tctcttcctc ctgctactct ggctcccaga taccaccgga 60 gatattgtga tgacccagac tccactctcc ctgcccgtca cccctggaga gccggcctcc 120 atctcctgca ggtctagtcg gagcctcttg gatagtgatg atggaaacac ctatttggac 180 tggtacctgc agaagccagg gcagtctcca cagctcctga tctacacgct ttcctatcgg 240 -104.39-gcctctggag tcccagacag gttcagtggc agtgggtcag gcactgattt cacactgaaa 300 atcagcaggg tggaggctga ggatgttgga gtttattact gcatgcaacg tgtagagttt 360 cctatcacct tcggccaagg gacacgactg gagattaaag gtggtggtgg ttctggcggc 420 ggcggctccg gtggtggtgg ttcccaggtg cagctggtgg agtctggggg aggcgtggtc 480 cagcctggga ggtccctgag actctcctgt gcagcgtctg gattcatctt cagtcgctat 540 ggcatgcact gggtccgcca ggctccaggc aaggggctga aatgggtggc agttatatgg 600 tatgatggaa gtaataaact ctatgcagac tccgtgaagg gccgattcac catctccaga 660 gacaattcca agaacacgct gtatctgcaa atgaacagcc tgagagccga ggacacggct 720 gtgtattact gtgcgagaga ttactatgat aatagtagac atcactgggg gtttgactac 780 tggggccagg gaaccctggt caccgtctcc tcaggaggtg gtggatccga tatcaaactg 840 cagcagtcag gggctgaact ggcaagacct ggggcctcag tgaagatgtc ctgcaagact 900 tctggctaca cctttactag gtacacgatg cactgggtaa aacagaggcc tggacagggt 960 ctggaatgga ttggatacat taatcctagc cgtggttata ctaattacaa tcagaagttc 1020 aaggacaagg ccacattgac tacagacaaa tcctccagca cagcctacat gcaactgagc 1080 agcctgacat ctgaggactc tgcagtctat tactgtgcaa gatattatga tgatcattac 1140 tgccttgact actggggcca aggcaccact ctcacagtct cctcagtcga aggtggaagt 1200 ggaggttctg gtggaagtgg aggttcaggt ggagtcgacg acattcagct gacccagtct 1260 ccagcaatca tgtctgcatc tccaggggag aaggtcacca tgacctgcag agccagttca 1320 agtgtaagtt acatgaactg gtaccagcag aagtcaggca cctcccccaa aagatggatt 1380 tatgacacat ccaaagtggc ttctggagtc ccttatcgct tcagtggcag tgggtctggg 1440 acctcatact ctctcacaat cagcagcatg gaggctgaag atgctgccac ttattactgc 1500 caacagtgga gtagtaaccc gctcacgttc ggtgctggga ccaagctgga gctgaaatag 1560 <210> 111 <211> 499 <212> PRT
<213> Homo Sapiens <400> 111 Asp Ile Val Met Thr Gin Thr Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Arg Ser Leu Leu Asp Ser Asp Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Thr Leu Ser Tyr Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Arg Val Glu Phe Pro Ile Thr Phe Gly Gin Gly Thr Arg Leu Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gin Val Gin Leu Val Glu Ser Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ile Phe Ser Arg Tyr -104.40-Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Lys Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Asp Asn Ser Arg His His Trp Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys <210> 112 <211> 1635 <212> DNA
<213> Homo Sapiens -104.41-<400> 112 atggaaaccc cagcgcagct tctcttcctc ctgctactct ggctcccaga taccaccgga 60 gatattgtga tgacccagac tccactctcc ctgcccgtca cccctggaga gccggcctcc 120 atctcctgca ggtctagtcg gagcctcttg gatagtgatg atggaaacac ctatttggac 180 tggtacctgc agaagccagg gcagtctcca cagctcctga tctacacgct ttcctatcgg 240 gcctctggag tcccagacag gttcagtggc agtgggtcag gcactgattt cacactgaaa 300 atcagcaggg tggaggctga ggatgttgga gtttattact gcatgcaacg tgtagagttt 360 cctatcacct tcggccaagg gacacgactg gagattaaac tttccgcgga cgatgcgaaa 420 aaggatgctg cgaagaaaga tgacgctaag aaagacgatg ctaaaaagga cctgcaggtg 480 cagctggtgg agtctggggg aggcgtggtc cagcctggga ggtccctgag actctcctgt 540 gcagcgtctg gattcatctt cagtcgctat ggcatgcact gggtccgcca ggctccaggc 600 aaggggctga aatgggtggc agttatatgg tatgatggaa gtaataaact ctatgcagac 660 tccgtgaagg gccgattcac catctccaga gacaattcca agaacacgct gtatctgcaa 720 atgaacagcc tgagagccga ggacacggct gtgtattact gtgcgagaga ttactatgat 780 aatagtagac atcactgggg gtttgactac tggggccagg gaaccctggt caccgtctcc 840 tcaggaggtg gtggatccga tatcaaactg cagcagtcag gggctgaact ggcaagacct 900 ggggcctcag tgaagatgtc ctgcaagact tctggctaca cctttactag gtacacgatg 960 cactgggtaa aacagaggcc tggacagggt ctggaatgga ttggatacat taatcctagc 1020 cgtggttata ctaattacaa tcagaagttc aaggacaagg ccacattgac tacagacaaa 1080 tcctccagca cagcctacat gcaactgagc agcctgacat ctgaggactc tgcagtctat 1140 tactgtgcaa gatattatga tgatcattac tgccttgact actggggcca aggcaccact 1200 ctcacagtct cctcactttc cgcggacgat gcgaaaaagg atgctgcgaa gaaagatgac 1260 gctaagaaag acgatgctaa aaaggacctg gacattcagc tgacccagtc tccagcaatc 1320 atgtctgcat ctccagggga gaaggtcacc atgacctgca gagccagttc aagtgtaagt 1380 tacatgaact ggtaccagca gaagtcaggc acctccccca aaagatggat ttatgacaca 1440 tccaaagtgg cttctggagt cccttatcgc ttcagtggca gtgggtctgg gacctcatac 1500 tctctcacaa tcagcagcat ggaggctgaa gatgctgcca cttattactg ccaacagtgg 1560 agtagtaacc cgctcacgtt cggtgctggg accaagctgg agctgaaaga ttataaggac 1620 gatgatgaca aatag 1635 <210> 113 <211> 524 <212> PRT
<213> Homo Sapiens <400> 113 Asp Ile Val Met Thr Gin Thr Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Arg Ser Leu Leu Asp Ser Asp Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Thr Leu Ser Tyr Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Arg Val Glu Phe Pro Ile Thr Phe Gly Gin Gly Thr Arg Leu Glu Ile -104.42-Lys Leu Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala Lys Lys Asp Asp Ala Lys Lys Asp Asp Ala Lys Lys Asp Leu Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ile Phe Ser Arg Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Lys Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Asp Asn Ser Arg His His Trp Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Leu Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala Lys Lys Asp Asp Ala Lys Lys Asp Asp Ala Lys Lys Asp Leu Asp Ile Gln Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys -104.43-Leu Glu Leu Lys Asp Tyr Lys Asp Asp Asp Asp Lys <210> 114 <211> 169 <212> PRT
<213> Homo Sapiens <400> 114 Trp Vol Leu Ser Gin Val Gin Leu Gin Glu Ser Gly Pro Gly Leu Vol Lys Pro Ser Glu Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Vol Ser Ser Gly Gly Tyr Tyr Trp Ser Trp Ile Arg Gin Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly Phe Ile Tyr Tyr Thr Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys Ser Arg Val Ser Ile Ser Val Asp Thr Ser Lys Asn Gin Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Ala Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Asp Trp Ser Phe His Phe Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala <210> 115 <211> 168 <212> PRT
<213> Homo Sapiens <400> 115 Gin Leu Leu Gly Leu Leu Leu Leu Trp Phe Pro Gly Ala Arg Cys Asp Ile Gin Met Thr Gin Ser Pro Ser Ser Leu Ser Ala Ser Ile Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gin Gly Ile Arg Asn Asp Leu Gly Trp Tyr Gin Gin Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile Tyr Ala Ala Ser Ser Leu Gin Ser Gly Val Pro Ser Arg Phe Ser Gly Ser -104.44-Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln His Asn Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala <210> 116 <211> 156 <212> PRT
<213> Homo Sapiens <400> 116 Gln Cys Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asn Tyr Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Asn Ile Gln Gln Asp Gly Ser Glu Lys Tyr Tyr Val Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Trp Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Ser Gly Val Val Glu <210> 117 <211> 151 <212> PRT
<213> Homo Sapiens , <400> 117 Leu Leu Gly Leu Leu Met Leu Trp Val Pro Gly Ser Ser Gly Asp Ile -104.45-Val Met Thr Gin Thr Pro Leu Ser Ser Thr Val Ile Leu Gly Gin Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Val His Ser Asp Gly Asn Thr Tyr Leu Asn Trp Leu Gin Gin Arg Pro Gly Gin Pro Pro Arg Leu Leu Ile Tyr Met Ile Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ala Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Ala Thr Glu Ser Pro Gin Thr Phe Gly Gin Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gin Leu Lys Ser Gly Arg Ala Ser Val <210> 118 <211> 180 <212> PRT
<213> Homo Sapiens <220>
<223> Xaa = any amino acid <400> 118 Xaa Xaa Xaa Xaa Glu Gin Ser Gly Gly Gly Val Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Ala Trp Met Thr Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Glu Trp Val Gly Arg Ile Lys Arg Arg Thr Asp Gly Gly Thr Thr Asp Tyr Ala Ala Pro Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Leu Tyr Leu Gin Met Asn Asn Leu Lys Asn Glu Asp Thr Ala Val Tyr Tyr Cys Thr Ser Val Asp Asn Asp Val Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn -104.46-Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gin Ser Ser Gly Leu <210> 119 <211> 152 <212> PRT
<213> Homo Sapiens <220>
<223> Xaa = any amino acid <400> 119 Xaa Xaa Xaa Leu Thr Gin Ser Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Leu His Ser Asn Gly Tyr Asn Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Leu Gly Ser Asn Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Ile Gly Leu Tyr Tyr Cys Met Gin Ala Leu Gin Thr Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Asp Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gin Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gin <210> 120 <211> 179 <212> PRT
<213> Homo Sapiens <400> 120 Gin Val Gin Leu Glu Gin Ser Gly Gly Gly Leu Val Gin Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr Ser Met Asn Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Tyr Ile Arg Ser Ser Thr Ser Thr Ile Tyr Tyr Ala Glu Ser Leu -104.47-Lys Gly Arg Phe Thr Ile Ser Ser Asp Asn Ala Lys Asn Ser Leu Tyr Leu Gin Met Asn Ser Leu Arg Asp Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Phe Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gin Ser Ser Gly Leu Tyr Ser Leu Ser <210> 121 <211> 163 <212> PRT
<213> Homo Sapiens <400> 121 Glu Ile Gin Leu Thr Gin Ser Pro Leu Ser Ser Pro Val Thr Leu Gly Gin Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Val His Ser Asp Gly Asp Thr Tyr Leu Asn Trp Leu Gin Gin Arg Pro Gly Gin Pro Pro Arg Leu Leu Ile Tyr Lys Ile Ser Thr Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ala Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Thr Asp Asp Val Gly Ile Tyr Tyr Cys Met Gin Thr Thr Gin Ile Pro Gin Ile Thr Phe Gly Gin Gly Thr Arg Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gin Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gin Ser Gly <210> 122 -104.48-<211> 189 <212> PRT
<213> Homo Sapiens <400> 122 Gin Val Gin Leu Glu Gin Ser Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Arg Tyr Gly Met His Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Lys Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Asp Asn Ser Arg His His Trp Gly Phe Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gin Ser Ser Gly Leu Tyr Ser Leu Ser <210> 123 <211> 159 <212> PRT
<213> Homo Sapiens <400> 123 Asp Ile Gin Leu Met Thr Leu Gin Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gin Ser Ile Tyr Ser Tyr Leu Asn Trp Tyr Gin Gin Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gin Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gin Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gin Gin Ser Tyr Ser Thr Pro Pro Thr Phe Gly Gin Gly Thr Lys Val Glu Ile Lys Arg Thr Val -104.49-Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gin Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gin Trp Lys Val Asp Asn Ala Leu Gin Ser Gly <210> 124 <211> 181 <212> PRT
<213> Homo Sapiens <220>
<223> Xaa = any amino acid <400> 124 Xaa Xaa Xaa Xaa Xaa Gin Ser Gly Gly Gly Leu Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Ala Trp Met Thr Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Glu Trp Val Gly Arg Ile Lys Arg Lys Thr Asp Gly Gly Thr Thr Asp Tyr Ala Ala Pro Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Glu Asn Thr Leu Tyr Leu Gin Met Asn Ser Leu Glu Thr Glu Asp Thr Ala Val Tyr Tyr Cys Thr Thr Val Asp Asn Ser Gly Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gin Ser Ser Gly Leu Ser <210> 125 <211> 159 <212> PRT
<213> Homo Sapiens <220>
-104.50-<223> Xaa = any amino acid <400> 125 Xaa Xaa Xaa Xaa Thr Gin Ser Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Leu His Ser Asn Gly Tyr Asn Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Leu Gly Ser Asn Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Ala Leu Gin Thr Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gin Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gin Trp Lys Val Asp Asn Ala Leu <210> 126 <211> 179 <212> PRT
<213> Homo Sapiens <400> 126 Gin Val Gin Leu Glu Gin Ser Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asn Tyr Gly Leu His Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Asp Trp Val Ala Val Ile Trp Tyr Asp Gly Ser His Lys Phe Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Phe Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Thr Arg Asp Leu Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr -104.51-Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser <210> 127 <211> 160 <212> PRT
<213> Homo Sapiens <400> 127 Glu Thr Gin Leu Thr Gin Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg Val Thr Leu Ser Cys Arg Ala Ser Gin Ser Val Ser Asn Asn Tyr Leu Ala Trp Tyr Gin Gin Lys Pro Gly Gin Ala Pro Arg Leu Leu Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro Glu Asp Cys Ala Glu Cys Tyr Cys Gin Gin Tyr Gly Ser Ser Leu Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gin Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gin Trp Glu Gly Gly Ile Thr Pro Ser Asn Arg Val <210> 128 <211> 182 <212> PRT
<213> Homo Sapiens <400> 128 Val Gin Cys Gin Val Gin Leu Val Glu Ser Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Her Ser Tyr Gly Met His Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Val Ile Trp Tyr Asp Gly Ser His Lys Tyr Leu Tyr Ala Thr Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser -104.52-Lys Asn Thr Leu Tyr Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser Ala Arg Asp Tyr Tyr Asp Thr Ser Arg His His Trp Gly Phe Asp Cys Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro <210> 129 <211> 173 <212> PRT
<213> Homo Sapiens <400> 129 Gin Leu Leu Gly Leu Leu Met Leu Trp Val Pro Gly Ser Ser Glu Glu Ile Val Met Thr Gin Thr Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gin Ser Leu Leu Asp Ser Glu Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Thr Leu Ser His Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Cys Cys Met Gin Arg Val Glu Phe Pro Ile Thr Phe Gly Gin Gly Thr Arg Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gin Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn <210> 130 <211> 187 <212> PRT
-104.53-<213> Homo Sapiens <220>
<223> Xaa = any amino acid <400> 130 Xaa Xaa Xaa Xaa Xaa Gln Ser Gly Pro Arg Leu Val Lys Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser Asp Gly Tyr Tyr Trp Ser Trp Ile Arg Gln His Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Phe Tyr Asn Pro Ser Leu Lys Ser Arg Val Ala Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu Ser Pro His Ser Ser Asn Trp Tyr Ser Gly Phe Asp Cys Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Arg Thr Gly Asp Gly Val Val Glu Leu Arg Arg Pro Asp Gln Arg Arg Ala His Leu Pro Gly Cys Pro Thr Val Leu Arg Thr Leu <210> 131 <211> 154 <212> PRT
<213> Homo Sapiens <220>
<223> Xaa = any amino acid <400> 131 Xaa Xaa Xaa Xaa Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Gly Ser Arg Leu His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile Lys Tyr Ala Ser Gln Ser Phe Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala -104.54-Glu Asp Ala Ala Thr Tyr Tyr Cys His Gin Ser Ser Asn Leu Pro Phe Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gin Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gin Trp Lys Val Asp Asn Ala Leu <210> 132 <211> 180 <212> PRT
<213> Homo Sapiens <400> 132 Gin Val Gin Leu Val Glu Gin Ala Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Arg Ser Tyr Gly Met His Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Lys Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Tyr Leu Tyr Thr Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg Asp Tyr Tyr Asp Asn Ser Arg His His Trp Gly Phe Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Arg Arg Arg Ala His Leu Pro Gly <210> 133 <211> 156 <212> PRT
<213> Homo Sapiens -104.55-<400> 133 Asp Ile Gin Met Thr Gin Ser Pro Ser Ser Arg Cys Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gin Gly Ile Arg Asn Asp Leu Ala Trp Tyr Gin Gin Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile Tyr Ala Ala Ser Ser Leu Gin Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gin Pro Glu Asp Phe Ala Ala Tyr Tyr Cys Leu Gin His Asn Ser Tyr Pro Pro Ser Phe Gly Gin Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gin Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gin Trp Lys Val Asp Asn Ala Leu Gin Ser <210> 134 <211> 171 <212> PRT
<213> Homo Sapiens <400> 134 His Val Gin Val Gin Leu Val Glu Ser Gly Gly Gly Val Val Gin Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ile Phe Ser Arg Tyr Gly Met His Trp Val Arg Gin Ala Pro Gly Lys Gly Leu Lys Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gin Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Asp Asn Ser Arg His His Trp Gly Phe Asp Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu -104.56-<210> 135 <211> 174 <212> PRT
<213> Homo Sapiens <400> 135 Ser Ala Pro Gly Ala Ala Asn Ala Leu Gly Pro Trp Ile Ser Glu Asp Ile Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Arg Ser Leu Leu Asp Ser Asp Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Thr Leu Ser Tyr Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Arg Val Glu Phe Pro Ile Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala <210> 136 <211> 1428 <212> DNA
<213> Homo Sapiens <400> 136 cggccgccta tttacccaga gacagggaga ggctcttctg tgtgtagtgg ttgtgcagag 60 cctcatgcat cacggagcat gagaagacat tcccctcctg ccacctgctc ttgtccacgg 120 ttagcctgct gtagaggaag aaggagccgt cggagtccag cacgggaggc gtggtcttgt 180 agttgttctc cggctgccca ttgctctccc actccacggc gatgtcgctg gggtagaagc 240 ctttgaccag gcaggtcagg ctgacctggt tcttggtcat ctcctcctgg gatgggggca 300 gggtgtacac ctgtggctct cggggctgcc ctttggcttt ggagatggtt ttctcgatgg 360 aggacgggag gcctttgttg gagaccttgc acttgtactc cttgccgttc agccagtcct 420 ggtgcaggac ggtgaggacg ctgaccacac ggtacgtgct gttgaactgc tcctcccgcg 480 gctttgtctt ggcattatgc acctccacgc catccacgta ccagttgaac tggacctcgg 540 ggtcttcctg gctcacgtcc accaccacgc acgtgacctc aggggtccgg gagatcatga 600 gagtgtcctt gggttttggg gggaacagga agactgatgg tccccccagg aactcaggtg 660 -104.57-ctgggcatga tgggcatggg ggaccatatt tggactcaac tctcttgtcc accttggtgt 720 tgctgggctt gtgatctacg ttgcaggtgt aggtcttcgt gcccaagctg ctggagggca 780 cggtcaccac gctgctgagg gagtagagtc ctgaggactg taggacagcc gggaaggtgt 840 gcacgccgct ggtcagggcg cctgagttcc acgacaccgt caccggttcg gggaagtagt 900 ccttgaccag gcagcccagg gcggctgtgc tctcggaggt gctcctggag cagggcgcca 960 gggggaagac ggatgggccc ttggtggaag ctgaggagac ggtgaccagg gttccctggc 1020 cccagtagtc aaacccccag tgatgtctac tattatcata gtaatctctc gcacagtaat 1080 acacagccgt gtcctcggct ctcaggctgt tcatttgcag atacagcgtg ttcttggaat 1140 tgtctctgga gatggtgaat cggcccttca cggagtctgc atagagttta ttacttccat 1200 cataccatat aactgccacc catttcagcc ccttgcctgg agcctggcgg acccagtgca 1260 tgccatagcg actgaagatg aatccagacg ctgcacagga gagtctcagg gacctcccag 1320 gctggaccac gcctccccca gactccacca gctgcacctg acactggaca ccttttaaaa 1380 tagccacaag aaaaagccag ctcagcccaa actccatggt ggtcgact 1428 <210> 137 <211> 469 <212> PRT
<213> Homo Sapiens =
<400> 137 Met Glu Phe Gly Leu Ser Trp Leu Phe Leu Val Ala Ile Leu Lys Gly Val Gln Cys Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ile-Phe Ser Arg Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Lys Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Asp Asn Ser Arg His His Trp Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val -104.58-Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gin Glu Asp Pro Glu Val Gin Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gin Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gin Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gin Pro Arg Glu Pro Gin Val Tyr Thr Leu Pro Pro Ser Gin Glu Glu Met Thr Lys Asn Gin Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gin Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gin Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gin Lys Ser Leu Ser Leu Ser Leu Gly Lys <210> 138 <211> 741 <212> DNA
<213> Homo Sapiens <400> 138 agtcgaccac catggaaacc ccagcgcagc ttctcttcct cctgctactc tggctcccag 60 ataccaccgg agatattgtg atgacccaga ctccactctc cctgcccgtc acccctggag 120 agccggcctc catctcctgc aggtctagtc ggagcctctt ggatagtgat gatggaaaca 180 cctatttgga ctggtacctg cagaagccag ggcagtctcc acagctcctg atctacacgc 240 tttcctatcg ggcctctgga gtcccagaca ggttcagtgg cagtgggtca ggcactgatt 300 tcacactgaa aatcagcagg gtggaggctg aggatgttgg agtttattac tgcatgcaac 360 gtgtagagtt tcctatcacc ttcggccaag ggacacgact ggagattaaa cgaactgtgg 420 ctgcaccatc tgtcttcatc ttcccgccat ctgatgagca gttgaaatct ggaactgcct 480 ctgttgtgtg cctgctgaat aacttctatc ccagagaggc caaagtacag tggaaggtgg 540 ataacgccct ccaatcgggt aactcccagg agagtgtcac agagcaggac agcaaggaca 600 gcacctacag cctcagcagc accctgacgc tgagcaaagc agactacgag aaacacaaag 660 tctacgcctg cgaagtcacc catcagggcc tgagctcgcc cgtcacaaag agcttcaaca 720 -104.59-ggggagagtg ttaggcggcc g 741 <210> 139 <211> 240 <212> PRT
<213> Homo Sapiens <400> 139 Met Glu Thr Pro Ala Gin Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro Asp Thr Thr Gly Asp Ile Val Met Thr Gin Thr Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Arg Ser Leu Leu Asp Ser Asp Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu Ile Tyr Thr Leu Ser Tyr Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin Arg Val Glu Phe Pro Ile Thr Phe Gly Gin Gly Thr Arg Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gin Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gin Trp Lys Val Asp Asn Ala Leu Gin Ser Gly Asn Ser Gin Glu Ser Val Thr Glu Gin Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gin Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys <210> 140 <211> 186 <212> PRT
<213> Homo Sapiens <220>
<223> Xaa = any amino acid <400> 140 Xaa Xaa Xaa Xaa Glu Gin Ser Gly Gly Gly Val Val Gin Pro Gly Arg -104.60-Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Phe Tyr Asp Ser Ser Arg Tyr His Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Ser <210> 141 <211> 143 <212> PRT
<213> Homo Sapiens <220>
<223> Xaa = any amino acid <400> 141 Xaa Xaa Xaa Xaa Thr Gln Cys Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Asp Ser Asp Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Thr Val Ser Tyr Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Arg Ile Glu Phe Pro Ile Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp -104.61-Glu Gin Leu Lys Set Gly Thr Ala Ser Val Val Cys Leu Leu Asn -104.62-

Claims (30)

What is claimed is:
1. An isolated human antibody, or binding fragment thereof, that specifically binds to T cell, immunoglobulin domain and mucin domain 1 (TIM-1), wherein said antibody or binding fragment thereof comprises a heavy chain amino acid sequence comprising three complementarity determining regions (CDRs) and a light chain amino acid sequence comprising three CDRs, where the three heavy chain CDRs and the three light chain CDRs are selected from:
(a) a sequence that is identical to a heavy chain CDR1 comprising the amino acid sequence GFIFSRYGMH, a sequence that is identical to a heavy chain CDR2 comprising the amino acid sequence VIWYDGSNKLYADSVKG, a sequence that is identical to a heavy chain CDR3 comprising the amino acid sequence DYYDNSRHHWGFDY, a sequence that is identical to a light chain CDR1 comprising the amino acid sequence RSSRSLLDSDDGNTYLD, a sequence that is identical to a light chain CDR2 comprising the amino acid sequence TLSYRAS, and a sequence that is identical to a light chain CDR3 comprising the amino acid sequence MQRVEFPIT;
(b) a sequence that is identical to a heavy chain CDR1 comprising the amino acid sequence GFTFTNYGLH, a sequence that is identical to a heavy chain CDR2 comprising the amino acid sequence VIWYDGSHKFYADSVKG, a sequence that is identical to a heavy chain CDR3 comprising the amino acid sequence DLDY, a sequence that is identical to a light chain CDR1 comprising the amino acid sequence RASQSVSNNYLA, a sequence that is identical to a light chain CDR2 comprising the amino acid sequence GASSRAT, and a sequence that is identical to a light chain CDR3 comprising the amino acid sequence QQYGSSLPLT;
(c) a sequence that is identical to a heavy chain CDR1 comprising the amino acid sequence GFTFSSYGMY, a sequence that is identical to a heavy chain CDR2 comprising the amino acid sequence VIWYDGSNKYYADSVKG, a sequence that is identical to a heavy chain CDR3 comprising the amino acid sequence DFYDSSRYHYGMDV, a sequence that is identical to a light chain CDR1 comprising the amino acid sequence RSSQSLLDSDDGNTYLD, a sequence that is identical to a light chain CDR2 comprising the amino acid sequence TVSYRAS, and a sequence that is identical to a light chain CDR3 comprising the amino acid sequence MQRIEFPIT;
(d) a sequence that is identical to a heavy chain CDR1 comprising the amino acid sequence GGSISSDGYYWS, a sequence that is identical to a heavy chain CDR2 comprising the amino acid sequence YIYYSGSTFYNPSLKS, a sequence that is identical to a heavy chain CDR3 comprising the amino acid sequence ESPHSSNWYSGFDC, a sequence that is identical to a light chain CDR1 comprising the amino acid sequence RASQSIGSRLH, a sequence that is identical to a light chain CDR2 comprising the amino acid sequence YASQSFS, and a sequence that is identical to a light chain CDR3 comprising the amino acid sequence HQSSNLPFT;
(e) a sequence that is identical to a heavy chain CDR1 comprising the amino acid sequence GFTFSRYGMH, a sequence that is identical to a heavy chain CDR2 comprising the amino acid sequence VIWYDGSNKLYADSVKG, a sequence that is identical to a heavy chain CDR3 comprising the amino acid sequence DYYDNSRHHWGFDY, a sequence that is identical to a light chain CDR1 comprising the amino acid sequence RASQSIYSYLN, a sequence that is identical to a light chain CDR2 comprising the amino acid sequence AASSLQS, and a sequence that is identical to a light chain CDR3 comprising the amino acid sequence QQSYSTPPT;
(f) a sequence that is identical to a heavy chain CDR1 comprising the amino acid sequence GFTFRSYGMH, a sequence that is identical to a heavy chain CDR2 comprising the amino acid sequence VIWYDGSNKYYTDSVKG, a sequence that is identical to a heavy chain CDR3 comprising the amino acid sequence DYYDNSRHHWGFDY, a sequence that is identical to a light chain CDR1 comprising the amino acid sequence RASQGIRNDLA, a sequence that is identical to a light chain CDR2 comprising the amino acid sequence AASSLQS, and a sequence that is identical to a light chain CDR3 comprising the amino acid sequence LQHNSYPPS;
(g) a sequence that is identical to a heavy chain CDR1 comprising the amino acid sequence GFTFSSYGMH, a sequence that is identical to a heavy chain CDR2 comprising the amino acid sequence VIWYDGSHKYYADSVKG, a sequence that is identical to a heavy chain CDR3 comprising the amino acid sequence DYYDTSRHHWGFDC, a sequence that is identical to a light chain CDR1 comprising the amino acid sequence RSSQSLLDSEDGNTYLD, a sequence that is identical to a light chain CDR2 comprising the amino acid sequence TLSHRAS, and a sequence that is identical to a light chain CDR3 comprising the amino acid sequence MQRVEFPIT;
(h) a sequence that is identical to a heavy chain CDR1 comprising the amino acid sequence GFTFSNAWMT, a sequence that is identical to a heavy chain CDR2 comprising the amino acid sequence RIKRRTDGGTTDYAAPVKG, a sequence that is identical to a heavy chain CDR3 comprising the amino acid sequence VDNDVDY, a sequence that is identical to a light chain CDR1 comprising the amino acid sequence RSSQSLLHSNGYNYLD, a sequence that is identical to a light chain CDR2 comprising the amino acid sequence LGSNRAS, and a sequence that is identical to a light chain CDR3 comprising the amino acid sequence MQALQTPLT;
(i) a sequence that is identical to a heavy chain CDR1 comprising the amino acid sequence GGSVSSGGYYWS, a sequence that is identical to a heavy chain CDR2 comprising the amino acid sequence FIYYTGSTNYNPSLKS, a sequence that is identical to a heavy chain CDR3 comprising the amino acid sequence DYDWSFHFDY, a sequence that is identical to a light chain CDR1 comprising the amino acid sequence RASQGIRNDLG, a sequence that is identical to a light chain CDR2 comprising the amino acid sequence AASSLQS, and a sequence that is identical to a light chain CDR3 comprising the amino acid sequence LQHNSYPLT;
(i) a sequence that is identical to a heavy chain CDR1 comprising the amino acid sequence GFTFSNAWMT, a sequence that is identical to a heavy chain CDR2 comprising the amino acid sequence RIKRKTDGGTTDYAAPVKG, a sequence that is identical to a heavy chain CDR3 comprising the amino acid sequence VDNSGDY, a sequence that is identical to a light chain CDR1 comprising the amino acid sequence RSSQSLLHSNGYNYLD, a sequence that is identical to a light chain CDR2 comprising the amino acid sequence LGSNRAS, and a sequence that is identical to a light chain CDR3 comprising the amino acid sequence MQALQTPLT;
(k) a sequence that is identical to a heavy chain CDR1 comprising the amino acid sequence GFTFTNYWMS, a sequence that is identical to a heavy chain CDR2 comprising the amino acid sequence NIQQDGSEKYYVDSVRG, a sequence that is identical to a heavy chain CDR3 comprising the amino acid sequence WDY, a sequence that is identical to a light chain CDR1 comprising the amino acid sequence RSSQSLVHSDGNTYLN, a sequence that is identical to a light chain CDR2 comprising the amino acid sequence MISNRFS, and a sequence that is identical to a light chain CDR3 comprising the amino acid sequence MQATESPQT; and (1) a sequence that is identical to a heavy chain CDR1 comprising the amino acid sequence GFTFSTYSMN, a sequence that is identical to a heavy chain CDR2 comprising the amino acid sequence YIRSSTSTIYYAESLKG, a sequence that is identical to a heavy chain CDR3 comprising the amino acid sequence DFDY, a sequence that is identical to a light chain CDR1 comprising the amino acid sequence RSSQSLVHSDGDTYLN, a sequence that is identical to a light chain CDR2 comprising the amino acid sequence KISTRFS, and a sequence that is identical to a light chain CDR3 comprising the amino acid sequence MQTTQIPQIT.
2. The isolated antibody or binding fragment of Claim 1, wherein said antibody or binding fragment thereof comprises a heavy chain amino acid sequence comprising three complementarity determining regions (CDRs) and a light chain amino acid sequence comprising three CDRs, where the three heavy chain CDRs and the three light chain CDRs are selected from:
(a) to a heavy chain CDR1 comprising the amino acid sequence GFIFSRYGMH, a heavy chain CDR2 comprising the amino acid sequence VIWYDGSNKLYADSVKG, a heavy chain CDR3 comprising the amino acid sequence DYYDNSRHHWGFDY, a light chain CDR1 comprising the amino acid sequence RSSRSLLDSDDGNTYLD, a light chain CDR2 comprising the amino acid sequence TLSYRAS, and a light chain CDR3 comprising the amino acid sequence MQRVEFPIT;
(b) a heavy chain CDR1 comprising the amino acid sequence GFTFTNYGLH, a heavy chain CDR2 comprising the amino acid sequence VIWYDGSHKFYADSVKG, a heavy chain CDR3 comprising the amino acid sequence DLDY, a light chain CDR1 comprising the amino acid sequence RASQSVSNNYLA, a light chain CDR2 comprising the amino acid sequence GASSRAT, and a light chain CDR3 comprising the amino acid sequence QQYGSSLPLT;
(c) a heavy chain CDR1 comprising the amino acid sequence GFTFSSYGMY, a heavy chain CDR2 comprising the amino acid sequence VIWYDGSNKYYADSVKG, a heavy chain CDR3 comprising the amino acid sequence DFYDSSRYHYGMDV, a light chain CDR1 comprising the amino acid sequence RSSQSLLDSDDGNTYLD, a light chain CDR2 comprising the amino acid sequence TVSYRAS, and a light chain CDR3 comprising the amino acid sequence MQRIEFPIT;

(d) a heavy chain CDR1 comprising the amino acid sequence GGSISSDGYYWS, a heavy chain CDR2 comprising the amino acid sequence YIYYSGSTFYNPSLKS, a heavy chain CDR3 comprising the amino acid sequence ESPHSSNWYSGFDC, a light chain CDR1 comprising the amino acid sequence RASQSIGSRLH, a light chain CDR2 comprising the amino acid sequence YASQSFS, and a light chain CDR3 comprising the amino acid sequence HQSSNLPFT;
(e) a heavy chain CDR1 comprising the amino acid sequence GFTFSRYGMH, a heavy chain CDR2 comprising the amino acid sequence VIWYDGSNKLYADSVKG, a heavy chain CDR3 comprising the amino acid sequence DYYDNSRHHWGFDY, a light chain CDR1 comprising the amino acid sequence RASQSIYSYLN, a light chain CDR2 comprising the amino acid sequence AASSLQS, and a light chain CDR3 comprising the amino acid sequence QQSYSTPPT;
(f) a heavy chain CDR1 comprising the amino acid sequence GFTFRSYGMH, a heavy chain CDR2 comprising the amino acid sequence VIWYDGSNKYYTDSVKG, a heavy chain CDR3 comprising the amino acid sequence DYYDNSRHHWGFDY, a light chain CDR1 comprising the amino acid sequence RASQGIRNDLA, a light chain CDR2 comprising the amino acid sequence AASSLQS, and a light chain CDR3 comprising the amino acid sequence LQHNSYPPS;
(g) a heavy chain CDR1 comprising the amino acid sequence GFTFSSYGMH, a heavy chain CDR2 comprising the amino acid sequence VIWYDGSHKYYADSVKG, a heavy chain CDR3 comprising the amino acid sequence DYYDTSRHHWGFDC, a light chain CDR1 comprising the amino acid sequence RSSQSLLDSEDGNTYLD, a light chain CDR2 comprising the amino acid sequence TLSHRAS, and a light chain CDR3 comprising the amino acid sequence MQRVEFPIT;
(h) a heavy chain CDR1 comprising the amino acid sequence GFTFSNAWMT, a heavy chain CDR2 comprising the amino acid sequence RIKRRTDGGTTDYAAPVKG, a heavy chain CDR3 comprising the amino acid sequence VDNDVDY, a light chain CDR1 comprising the amino acid sequence RSSQSLLHSNGYNYLD, a light chain CDR2 comprising the amino acid sequence LGSNRAS, and a light chain CDR3 comprising the amino acid sequence MQALQTPLT;
(i) a heavy chain CDR1 comprising the amino acid sequence GGSVSSGGYYWS, a heavy chain CDR2 comprising the amino acid sequence FIYYTGSTNYNPSLKS, a heavy chain CDR3 comprising the amino acid sequence DYDWSFHFDY, a light chain CDR1 comprising the amino acid sequence RASQGIRNDLG, a light chain CDR2 comprising the amino acid sequence AASSLQS, and a light chain CDR3 comprising the amino acid sequence LQHNSYPLT;
(j) a heavy chain CDR1 comprising the amino acid sequence GFTFSNAWMT, a heavy chain CDR2 comprising the amino acid sequence RIKRKTDGGTTDYAAPVKG, a heavy chain CDR3 comprising the amino acid sequence VDNSGDY, a light chain CDR1 comprising the amino acid sequence RSSQSLLHSNGYNYLD, a light chain CDR2 comprising the amino acid sequence LGSNRAS, and a light chain CDR3 comprising the amino acid sequence MQALQTPLT;
(k) a heavy chain CDR1 comprising the amino acid sequence GFTFTNYWMS, a heavy chain CDR2 comprising the amino acid sequence NIQQDGSEKYYVDSVRG, a heavy chain CDR3 comprising the amino acid sequence WDY, a light chain CDR1 comprising the amino acid sequence RSSQSLVHSDGNTYLN, a light chain CDR2 comprising the amino acid sequence MISNRFS, and a light chain CDR3 comprising the amino acid sequence MQATESPQT; and (1) a heavy chain CDR1 comprising the amino acid sequence GFTFSTYSMN, a heavy chain CDR2 comprising the amino acid sequence YIRSSTSTIYYAESLKG, a heavy chain CDR3 comprising the amino acid sequence DFDY, a light chain CDR1 comprising the amino acid sequence RSSQSLVHSDGDTYLN, a light chain CDR2 comprising the amino acid sequence KISTRFS, and a light chain CDR3 comprising the amino acid sequence MQTTQIPQIT.
3. The isolated antibody or binding fragment of Claim 2, wherein said antibody or binding fragment comprises a heavy chain amino acid sequence and a light chain amino acid sequence selected from the group consisting of:
(a) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 26, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 28;
(b) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 46, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 48;
(c) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 34, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 36;
(d) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 42, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 44;
(e) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 18, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 20;
(f) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 38, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 40;
(g) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 30, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 32;

(h) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 10, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 12;
(i) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 2, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 4;
(j) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 22, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 24;
(k) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 6, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 8;
(l) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 14, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 16; and (m) a sequence that is identical to the heavy chain amino acid sequence of SEQ ID
NO: 50, and a sequence that is identical to the light chain amino acid sequence of SEQ ID NO: 52.
4. A human antibody or binding fragment thereof that competes for binding with a human antibody that binds to human TIM-1 and comprises a heavy chain amino acid sequence and a light chain amino acid sequence selected from the group consisting of:
(a) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 26, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 28;
(b) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 46, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 48;

(c) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 34, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 36;
(d) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 42, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 44;
(e) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 18, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 20;
(f) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 38, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 40;
(g) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 30, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 32;
(h) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 10, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 12;
(i) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 2, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 4;
(l) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 22, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 24;
(k) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 6, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 8;

(l) a sequence that is identical to the variable heavy chain amino acid sequence of SEQ ID NO: 14, and a sequence that is identical to the variable light chain amino acid sequence of SEQ ID NO: 16; and (m) a sequence that is identical to the heavy chain amino acid sequence of SEQ ID
NO: 50, and a sequence that is identical to the light chain amino acid sequence of SEQ ID NO: 52.
The antibody of Claim 1, wherein said antibody is a monoclonal antibody.
6. The antibody of Claim 1, wherein said binding fragment comprises a Fab, Fab', F (ab")2, or Fv fragment of said antibody.
7. The antibody of Claim 1, wherein said antibody is a single chain antibody.
8. The antibody, or binding fragment, of Claim 1, wherein said antibody or binding fragment is associated with a pharmaceutically acceptable carrier or diluent.
9. The antibody, or binding fragment of Claim 1, wherein the antibody or binding fragment is conjugated to a therapeutic agent.
10. The antibody, or binding fragment of Claim 9, wherein the therapeutic agent is a toxin, radioactive isotope or chemotherapeutic agent.
11. The antibody, or binding fragment of Claim 1, wherein said antibody binds to TIM-1 with a Kd between 10-7 and 10-14 M.
12. The antibody or binding fragment of Claim 1 for use in a method of inhibiting cancer cell proliferation associated with the expression of TIM-1 in a mammal.
13. The antibody or binding fragment of Claim 12, wherein the mammal is a human.
14. The antibody or binding fragment of Claim 12, wherein the cancer involves epithelial cell proliferation.
15. The antibody or binding fragment of Claim 14, wherein the cancer comprises a lung, colon, gastric, kidney, renal, prostate or ovarian carcinoma.
16. The antibody or binding fragment of Claim 14, wherein the mammal suffers from a neoplasm which may be selected from the group consisting of: renal and pancreatic tumors, head and neck cancer, ovarian cancer, gastric (stomach) cancer, melanoma, lymphoma, prostate cancer, liver cancer, lung cancer, renal (kidney) cancer, bladder cancer, colon cancer, esophageal cancer, endometrial cancer, salivary gland cancer, colorectal cancer, thyroid cancer, breast cancer, brain cancer and glioblastoma.
17. The antibody or binding fragment of Claim 14, wherein the mammal suffers from renal cancer.
18. The antibody or binding fragment of Claim 14, wherein the mammal suffers from ovarian cancer.
19. A hybridoma cell line producing the antibody, or binding fragment, of Claim 1.
20. A transformed cell comprising a nucleic acid encoding the antibody, or binding fragment, of Claim 1.
21. The transformed cell of Claim 20, wherein the cell is a Chinese hamster ovary (CHO) cell.
22. An ex vivo method of inhibiting cancer cell proliferation associated with the expression of TIM-1, comprising treating cells expressing TIM-1 with an effective amount of a human antibody, or binding fragment, of Claim 1.
23. An article of manufacture comprising a container, a composition contained therein, and a package insert or label indicating that the composition can be used to treat cancer characterized by the overexpression of TIM-1, wherein the composition comprises the antibody, or binding fragment, of Claim 1.
24. The article of manufacture of Claim 23, wherein the cancer is a lung, colon, gastric, kidney, renal, prostate or ovarian carcinoma.
25. An assay kit for the detection of TIM-1 in mammalian tissues or cells in order to screen for lung, colon, gastric, kidney, renal, prostate or ovarian carcinomas, the TIM-1 being an antigen expressed by lung, colon, gastric, kidney, renal, prostate or ovarian carcinomas, the kit comprising an antibody of claim 1 and means for indicating the reaction of the antibody with the antigen, if present.
26. The assay kit of Claim 25, wherein the antibody is a monoclonal antibody.
27. The assay kit of Claim 25, wherein the antibody that binds the antigen is labeled.
28. The assay kit of Claim 25, wherein the antibody is an unlabeled first antibody and the means for indicating the reaction comprises a labeled second antibody that is anti-immunoglobulin.
29. The assay kit of Claim 27, wherein the antibody that binds the antigen is labeled with a marker selected from the group consisting of a fluorochrome, an enzyme, a radionuclide and a radiopaque material.
30.
The assay kit of Claim 28, wherein the second antibody is labeled with a marker selected from the group consisting of a fluorochrome, an enzyme, a radionuclide and a radiopaque material.
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