CA2510446A1 - Genes conferring herbicide resistance - Google Patents

Genes conferring herbicide resistance Download PDF

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Publication number
CA2510446A1
CA2510446A1 CA002510446A CA2510446A CA2510446A1 CA 2510446 A1 CA2510446 A1 CA 2510446A1 CA 002510446 A CA002510446 A CA 002510446A CA 2510446 A CA2510446 A CA 2510446A CA 2510446 A1 CA2510446 A1 CA 2510446A1
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CA
Canada
Prior art keywords
seq
nucleotide sequence
nucleic acid
plant
sequence
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Granted
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CA002510446A
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French (fr)
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CA2510446C (en
Inventor
Philip E. Hammer
Nicholas B. Duck
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Athenix Corp
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Individual
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Publication of CA2510446A1 publication Critical patent/CA2510446A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate

Abstract

Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID N0:2 or the nucleotide sequence set forth in SEQ ID NO: 1.

Claims (15)

1. An isolated nucleic acid molecule selected from the group consisting of:
a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1 or a complement thereof;
b) a nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of SEQ ID
NO:1 or a complement thereof;
c) a nucleic acid molecule that encodes a polypeptide having the amino acid sequence of SEQ ID NO:2; and d) a nucleic acid molecule that encodes a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:2.
2. A vector comprising the nucleic acid molecule of claim 1.
3. A host cell that contains the vector of claim 2.
4. The host cell of claim 3 that is a bacterial host cell.
5. The host cell of clean 3 that is a plant host cell.
6. An isolated polypeptide selected from the group consisting of:
a) a polypeptide comprising the amino acid sequence of SEQ ID
NO:2;
b) a polypeptide having at least 95% sequence identity to the nucleotide sequence of SEQ ID NO:2.
7. An antibody that selectively binds to a polypeptide of claim 6.
8. A plant having stably incorporated into its genome a DNA construct comprising at least one nucleotide sequence selected from the group consisting of:

a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1 or a complement thereof;
b) a nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of SEQ ID
NO:1 or a complement thereof;
c) a nucleic acid molecule that encodes a polypeptide having the amino acid sequence of SEQ ID NO:2; and d) a nucleic acid molecule that encodes a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:2.
9. The plant of claim 8, wherein said plant is selected from the group consisting of corn, alfalfa, wheat, soybean, rice, Brassica, sunflower, cotton, peanut, sorghum, millet and tobacco.
10. The plant of claim 8, wherein said plant is a monocot.
11. The plant of claim 8, wherein said plant is a dicot.
12. Seed of the plant according to claim 8.
13. A plant cell having stably incorporated into its genome a DNA
construct comprising at least one nucleotide sequence selected from the group consisting of:
a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1 or a complement thereof;
b) a nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of SEQ ID
NO:1 or a complement thereof;
c) a nucleic acid molecule that encodes a polypeptide having the amino acid sequence of SEQ ID NO:2; and d) a nucleic acid molecule that encodes a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:2.
14. A method for conferring resistance to glyphosate in a plant, comprising:
a) stably integrating into the genome of a plant cell a DNA
construct comprising a promoter operably linked to a nucleotide sequence of interest, wherein said nucleotide sequence of interest is selected from the group consisting of:
i) the nucleotide sequence set forth in SEQ NO:1;
ii) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:2; and, iii) a nucleotide sequence that is 95% identical to SEQ ID
NO:1; and b) regenerating said cell into a plant.
15. A method for conferring resistance to glyphosate in a plant cell, comprising stably integrating into the genome of said plant cell a DNA
construct comprising a promoter operably linked to a nucleotide sequence of interest, wherein said nucleotide sequence of interest is selected from the group consisting of:
a) the nucleotide sequence set forth in SEQ NO:1;
b) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:2; and c) a nucleotide sequence that is 95% identical to SEQ ID NO:1.
CA2510446A 2002-12-18 2003-12-18 Genes conferring herbicide resistance Expired - Fee Related CA2510446C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US43478902P 2002-12-18 2002-12-18
US60/434,789 2002-12-18
PCT/US2003/040998 WO2004056179A2 (en) 2002-12-18 2003-12-18 Genes conferring herbicide resistance

Publications (2)

Publication Number Publication Date
CA2510446A1 true CA2510446A1 (en) 2004-07-08
CA2510446C CA2510446C (en) 2011-01-25

Family

ID=32682106

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2510446A Expired - Fee Related CA2510446C (en) 2002-12-18 2003-12-18 Genes conferring herbicide resistance

Country Status (10)

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US (3) US7405347B2 (en)
EP (1) EP1581642B1 (en)
CN (1) CN1745173B (en)
AT (1) ATE506438T1 (en)
AU (1) AU2003299832B2 (en)
BR (2) BRPI0317579B1 (en)
CA (1) CA2510446C (en)
DE (1) DE60336849D1 (en)
HK (1) HK1082271A1 (en)
WO (1) WO2004056179A2 (en)

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AU2003299832B2 (en) 2009-06-18
US7659376B2 (en) 2010-02-09
ATE506438T1 (en) 2011-05-15
WO2004056179A2 (en) 2004-07-08
US7399621B2 (en) 2008-07-15
US20080312419A1 (en) 2008-12-18
EP1581642A2 (en) 2005-10-05
AU2003299832A1 (en) 2004-07-14
US20040177399A1 (en) 2004-09-09
EP1581642B1 (en) 2011-04-20
EP1581642A4 (en) 2006-06-21
WO2004056179A9 (en) 2004-09-16
BRPI0317579B1 (en) 2019-09-03
HK1082271A1 (en) 2006-06-02
US7405347B2 (en) 2008-07-29
CN1745173B (en) 2012-11-28
CA2510446C (en) 2011-01-25
CN1745173A (en) 2006-03-08
WO2004056179A3 (en) 2005-05-26
US20070004907A1 (en) 2007-01-04
BR0317579A (en) 2005-11-22
DE60336849D1 (en) 2011-06-01

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