CA2500936A1 - Parallel loading of arrays - Google Patents

Parallel loading of arrays Download PDF

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Publication number
CA2500936A1
CA2500936A1 CA002500936A CA2500936A CA2500936A1 CA 2500936 A1 CA2500936 A1 CA 2500936A1 CA 002500936 A CA002500936 A CA 002500936A CA 2500936 A CA2500936 A CA 2500936A CA 2500936 A1 CA2500936 A1 CA 2500936A1
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Prior art keywords
arrays
sub
array
sample
microarray
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CA002500936A
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French (fr)
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CA2500936C (en
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Roland D. Green
Mark Mccormick
Gary Barrett
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Roche Sequencing Solutions Inc
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Individual
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
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    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B01J2219/00619—Delimitation of the attachment areas by chemical means using hydrophilic or hydrophobic regions
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
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Abstract

The present inventions provides an apparatus (20) and method for the paralle l loading of multiple samples in a microarray (10) containing a plurality of s ub- arrays (14). The method makes use of a microarray (10) containing multiple s ub- arrays (14), a loading channel array (20), and a fluid handling robot or an assembly robot or machine.

Claims (18)

1. A method for loading samples on a microarray which is formed of a plurality of sub-arrays on a common substrate, the method comprising the steps of placing the samples to be loaded onto a sample loading array, the samples located on the sample loading array in physical alignment with the location of the sub-arrays on the microarray;
and placing the sample loading array in contact with the microarray under conditions so that molecules in the samples can hybridize to probes in the aligned sub-arrays.
2. A microarray hybridization chamber for parallel loading of samples comprising:
a sample loading array comprising a plurality of micro-channels having a first open end on a first side of the channel array and a second open end on a second side of the channel array, said first end and second end defining a channel in fluid communication with the first side of the channel array and the second side of the channel area, each channel having a longitudinal axis passing through its center and defining the center of the channel;
a microarray comprising a plurality of sub-arrays;
a membrane in contact with the second side of the channel array to close the second end of the micro-channels and to allow for the selective passing of liquids and molecules through the membrane and through the micro-channels; and a gasket, the gasket being placed between the first side of the channel array and the microarray so as to provide a hybridization chamber, and wherein the center of the channels are aligned with the center of the sub-arrays.
3. The chamber of Claim 2 wherein the gasket is dual sided and semi-adhesive.
4. The chamber of Claim 2 wherein the sub-arrays are divided by a hydrophobic barrier.
5. The chamber of Claim 4 wherein the hydrophobic barrier comprises of a hydrophobic group-bearing phosphoramidite.
6. The chamber of Claim 5 wherein the hydrophobic group-bearing phosphoramidite is trityl protected phosphoramidite.
7. A method for loading in parallel at least one sample into a plurality of sub-arrays, the method comprising the steps of:
providing a sample loading array comprising a plurality of micro-channels having a first open end on a first side of the sample loading array and a second open end on a second side of the sample array, each channel having a longitudinal axis passing through its center and defining the center of the channel;
providing a microarray comprising a plurality of sub-arrays;
providing a membrane in contact with the second side of the sample loading array to close the second end of at least one micro-channel and to allow for the selective passing of liquids and molecules through the membrane and through the micro-channels;
depositing a sample in at least one micro-channel through its first open end;
and bringing the sample array into contact with the microarray to contact the samples placed in the micro-channels to the corresponding sub-array.
8. The method of Claim 7 wherein the samples are placed in contact with the subarray using either centrifugal force or pressure.
9. The method of Claim 7 wherein the samples are placed in contact with the subarray using a vacuum.
10. The method of Claim 7 wherein there is a gasket located between the sample loading array and the microarray.
11. The method of Claim 7 wherein the sub-arrays are divided by a hydrophobic barrier.
12. The method of Claim 11 wherein the hydrophobic barrier comprises of a hydrophobic group-bearing phosphoramidite.
13. The method of Claim 7 wherein the sample is deposited into the plurality of micro-channels using a delivery system capable of simultaneous delivery of samples to multiple sites.
14. A method for simultaneously hybridizing a microarray having multiple sub-arrays, the method comprising the steps of:
providing a first microarray which includes a plurality of sub-arrays;
depositing a sample for each sub-array on a planar sample loading array;
placing against the microarray a gasket to encompass the sub-arrays containing the sample; and placing in contact with the gasket the sample loading array with each sample aligned with a one of the sub-arrays as to provide a sandwich hybridization chamber.
15. The method of Claim 14 wherein the sub-arrays are divided by a hydrophobic barrier.
16. The method of Claim 15 wherein the hydrophobic barrier is formed by a hydrophobic group-bearing phosphoramidite bound to the substrate.
17. The method of Claim 14 wherein the sample is deposited into a plurality of sub-arrays using a delivery system capable of simultaneous delivery of samples to multiple sites.
18. The method of Claim 14 wherein the delivery system is either a bundle of capillary tubes, a fluid handling robot, or a robot designed for manufacturing spotted arrays.
CA2500936A 2002-09-30 2003-09-30 Parallel loading of arrays Expired - Fee Related CA2500936C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US41511802P 2002-09-30 2002-09-30
US60/415,118 2002-09-30
US41509002P 2002-10-01 2002-10-01
US60/415,090 2002-10-01
PCT/US2003/030841 WO2004031399A2 (en) 2002-09-30 2003-09-30 Parallel loading of arrays

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Publication Number Publication Date
CA2500936A1 true CA2500936A1 (en) 2004-04-15
CA2500936C CA2500936C (en) 2012-10-30

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US (2) US7482170B2 (en)
JP (1) JP4471927B2 (en)
AU (1) AU2003282885A1 (en)
CA (1) CA2500936C (en)
DE (1) DE10393406T5 (en)
GB (1) GB2409455B (en)
WO (1) WO2004031399A2 (en)

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