CA2484676A1 - Kinase anchor protein muteins, peptides thereof, and related methods - Google Patents

Abstract

A-kinase anchor protein (AKAPs) muteins, peptides thereof, and nucleic acids encoding the peptides are provided herein. Also provided are transgenic animals, cells comprising transgenes and various methods employing such peptides.

Description

KINASE ANCHOR PROTEIN MUTEINS, PEPTIDES THEREOF, AND RELATED METHODS
GRANTS
Work described herein was supported by NIH grants DK-54441 and 5T32 DK-07233. The government may have certain rights in subject matter provided herein.
RELATED APPLICATIONS
Benefit of priority is claimed to U.S. Provisional Application Serial No.
60/377,852, entitled "Kinase Anchor Protein Muteins, Peptides Thereof, and Related Methods", filed May 3, 2002, attorney docket number 24736-P2065;
and to U.S. Provisional Application Serial No. 60/453,408, entitled "Kinase Anchor Protein Muteins, Peptides Thereof, and Related Methods", filed March 7, 2003, attorney docket number 24736-P2065B. This application is also related to U.S. application Serial No. (attorney docket no. 24736-2065), filed on the same day herewith, entitled "Kinase Anchor Protein Muteins, Peptides Thereof, and Related Methods." Where permitted, the subject matter of each of these provisional applications and the U.S. application is incorporated in its entirety by reference thereto.
FIELD OF THE INVENTION
A-kinase anchor protein (AKAPs) muteins, peptides thereof, and nucleic acids encoding the peptides are provided herein.
BACKGROUND OF THE INVENTION
Protein phosphorylation is an important mechanism for enzyme regulation and the transduction of extracellular signals across the cell membrane in eukaryotic cells. A wide variety of cellular substrates, including enzymes, membrane receptors, ion channels and transcription factors, can be phosphorylated in response to extracellular signals that interact with cells.
A
key enzyme in the phosphorylation of cellular proteins in response to hormones and neurotransmitters is cyclic AMP (CAMP)-dependent protein kinase (PKA).
Upon activation by cAMP, PKA thus mediates a variety of cellular responses to such extracellular signals. An array of PKA isozymes are expressed in mammalian cells. The PKA holoenzymes usually exist as inactive tetramers

-2-containing a regulatory (R) subunit dimer and two catalytic (C) subunits.
Genes encoding three C subunits (Ca, C/3 and Cy) and four R subunits (Rla, RIR, Rlla and RII/3) have been identified (see Takio et al. (1982) Proc. Nat/. Acad.
Sci.
USA, 79:2544-2548; Lee et al. (1983) Proc. Nat/. Acad. Sci. USA, 80:3608-3612; Jahnsen et al. (1996) J. Biol. Chem., 261 :12352-12361; Clegg et al.
(1988) Proc. Nat/. Acad. Sci. USA, 85:3703-3707; and Scott (1991 ) Pharmacol.
Ther., 50:123-145). The type I (RI) a and type II (RII) a subunits are distributed ubiquitously, whereas Rla and RII/3 are present mainly in brain (see. e.g., Miki and Eddy (1999) J. Biol. Chem., 274:29057-29062). The type I PKA
holoenzyme (Rla and RIR) is predominantly cytoplasmic, whereas the majority of type II PKA (Rlla and RII/3) associates with cellular structures and organelles (Scott (1991 ) Pharmacol. Ther., 50:123-145). Many hormones and other signals act through receptors to generate CAMP which binds to the R subunits of PKA and releases and activates the C subunits to phosphorylate proteins.
Because protein kinases and their substrates are widely distributed throughout cells, there are mechanisms in place in cells to localize protein kinase-mediated responses to different signals. One such mechanism involves subcellular targeting of PKAs through association with anchoring proteins, referred to as A-kinase anchoring proteins (AKAPs), that place PKAs in close proximity to specific organelles or cytoskeletal components and particular substrates, thereby providing for more specific PKA interactions and localized responses (see, e.g., Scott et al. (1990) J. Biol. Chem., 265:21561-21566;
Bregman et al. (1991 ) J. Biol. Chem., 266:7207-7213; and Miki and Eddy (1999) J. Biol. Chem., 274:29057-29062). Anchoring not only places the kinase close to preferred substrates, but also positions the PKA holoenzyme at sites where it can optimally respond to fluctuations in the second messenger cAMP (Mochly-Rosen (1995) Science, 268:247-251; Faux and Scott (1996) Trends Biochem. Sci., 21 :312-315; Hubbard and Cohen (1993) Trends Biochem.
Sci., 18:172-177).
Up to 75% of type II PKA is localized to various intracellular sites through association of the regulatory subunit (RII) with AKAPs (see, e.g., Hausken et al.
(1996) J. Biol. Chem., 271 :29016-29022). RII subunits of PKA bind to AKAPs

-3-with nanomolar affinity (Cart et al. (1992) J. Biol. Chem., 267:13376-13382), and many AKAP-RII complexes have been isolated from cell extracts. RI
subunits of PKA bind to AKAPs with only micromolar affinity (Burton et al.
(1997) Proc. Nat/. Acad. Sci. USA, 94:11067-11072). Evidence of binding of a PKA RI subunit to an AKAP has been reported (Miki and Eddy (1998) J. Biol.
Chem., 273:34384-34390) in which Rla-specific and Rla/Rlla dual specificity PKA anchoring domains were identified on FSC1 /AKAP82. Additional dual specific AKAPs, referred to as D-AKAP1 and D-AKAP2, which interact with the type I and type II regulatory subunits of PKA have also been reported (Huang et a/. (1997) J. Biol. Chem., 272:8057-8064; Huang et al. (1997) Proc. Nat/.
Acad.
Sci. USA, 94:1 1184-1 1189).
More than 20 AKAPs have been reported in different tissues and species.
Complementary DNAs (cDNAs) encoding AKAPs have been isolated from diverse species, ranging from Caenorhahditis alegans and Drosophilia to human (see, e.g., Colledge and Scott (1999) Trends Cell Biol., 9:216-221 ). Regions within AKAPs that mediate association with RII subunits of PKA have been identified.
These regions of approximately 10-18 amino acid residues vary substantially in primary sequence, but secondary structure predictions indicate that they are likely to form an amphipathic helix with hydrophobic residues aligned along one face of the helix and charged residues along the other (Cart et al. (1991 ) J.
Biol.
Chem., 266:14188-14192; Carr et al. (1992) J. Biol. Chem., 267:13376-13382). Hydrophobic amino acids with a long aliphatic side chain, e.g., valine, leucine or isoleucine, may participate in binding to RII subunits (Glantz et al.
(1993) J. Biol. Chem., 268:12796-12804).
Many AKAPs also have the ability to bind to multiple proteins, including other signalling enzymes. For example, AKAP79 binds to PKA, protein kinase C
(PKC) and the protein phosphatase calcineurin (PP2B) (Coghlan et al. (1995) Science, 267:108-1 12 and Klauck et al. (1996) Science, 271 :1589-1592).
Therefore, the targeting of AKAP79 to neuronal postsynaptic membranes brings together enzymes with opposite catalytic activities in a single complex.
AKAPs thus serve as potential regulatory mechanisms that increase the selectivity and intensity of a CAMP-mediated response. There is a need,

-4-therefore, to identify and elucidate the structural and functional properties of AKAPs in order to gain a complete understanding of the important role these proteins play in the basic functioning of cells.
SUMMARY OF THE INVENTION
Provided herein are D-AKAP2 peptide muteins that exhibit modified binding to a regulatory subunit of PKA compared to native D-AKAP2. Also provided herein are transgenic animals and cells comprising the peptides provided herein. In one embodiment, the peptides exhibit a preferred or exclusive binding to PKA-Rla subunits relative to PKA-Rlla subunits; or enhanced binding to both Rla and Rlla subunits. In another embodiment, the peptides exhibit a preferred or exclusive binding to PKA-Rlla subunits relative to PKA-Rla subunits.
The peptides provided herein are useful to disrupt, e.g., in vitro, the binding of particular isoforms of PKA, e.g., PKA-Rla or PKA-Rlla, to D-AKAP2.
For example, peptides provided herein having enhanced ability to bind to either one of PKA-Rla or PKA-Rlla isoforms are useful to competitively bind to their target PKA isoform and so prevent binding of the particular PKA isoform to its target protein D-AKAP2. In a particular embodiment, the enhanced binder for one PKA isoform that binds weakly to the other isoform (e.g., VQGNTDEAQEELAWKIAKMIWSD[I/V]MQQ; SEQ ID NOs:54 and 101, which binds tightly to PKA-Rla and weakly to PKA-Rlla; see Table 8) is employed to specifically knock out a specific function of one particular isoform mediated by D-AKAP2.
The peptides provided herein that have a combination of one, two or more specific amino acid changes relative to unmodified native protein sequences can also be used to specifically design peptide mimetics (peptidomimetics) or other small molecules to modulate the D-AKAP2-mediated biological function within cells or organisms. This change of function is contemplated herein to treat medical conditions like heart failure, arrhythmias, or prevent sudden death syndrome. In addition, the binding properties of the peptides provided herein are useful to design and establish high-throughput assay systems to screen large chemical compound libraries for the purpose of

-5-drug discovery. Such assay systems are also useful to characterize chemically modified lead compounds after the initial high-throughput screening.
The Rla specific binding differences for the D-AKAP2 Ile/Val 27-mer peptide variants elucidated herein are contemplated as translating into one or more health risks. AKAPs in general coordinate signaling through PKA by bringing together effector molecules at specific sub-cellular locations (Edwards et a/. (2000) Current Opinion in Cell Biology, 12:217-221 ). The Ile(646) variant (SEQ ID N0:64), which is selected for in the healthy population, is contemplated herein to result in a reduction in signaling through the PKA Rla isoform. This signal reduction could have a beneficial effect on the cell. The narrow affinity differences observed for the binding of the variants to Rla suggests that local concentrations of Rla are tightly regulated. While AKAP specific anchoring of PKA through Rlla has been well documented, little was previously known about anchoring PKA through Rla. As provided herein, however, anchoring PKA
through the Rla subunit appears to be more dynamic. Rla is found diffuse in the cytoplasm of most cells, however several examples of Rla localization have been reported. Rla is recruited to the plasma membrane upon antigen-mediated lymphocyte activation (Skalhegg et al. (1994) Science, 263:84-87), localized to the neuromuscular junction of skeletal muscle (Barradeau et al. (2001 ) Proc.
Nat/, Acad. Sci. USA, 264:250-265), and associated with microtubules during certain stages of the cell cycle (Imaizumi et al. (2001 ) Experimental Cell Research, 264:250-265). Therefore, dynamic regulation between intracellular compartments seems to be key for Rla mediated signaling and might be different depending on the D-AKAP2 variants present in the cell. Accordingly, methods of modulating D-AKAP2-mediated intracellular compartmentalization of PKA are provided herein.
In addition to altering the dynamic nature of the Rla signaling pathway, the Ile/Val(646) variant of D-AKAP2 is contemplated herein to alter the PKA
isoform distribution and change the signaling specificity of PKA. D-AKAP2 can bind both RI and RII isoforms of PKA, potentially recruiting two different responses to CAMP signaling. The affinity of cAMP for Rla is higher than Rlla and requires lower concentrations of cAMP for PKA activation (Dostmann et al.

6 PCT/US03/13698 (1990) J. Biol, Chem., 265:10484-10491 ). Therefore, an Rla anchored PKA
isoform would be activated by a lower, transient concentration of cAMP as opposed to a higher, persistent concentration for Rlla (Feliciello et al.
(2001 ) J, Mol. Biol., 308:99-1 14). The tighter binding D-AKAP2 val(646) variant could potentially more effectively recruit Rla at the expense of the Rlla isoform, altering the isoform distribution and changing the cAMP mediated signaling response.
Increasing evidence indicates that the RI and RII isoforms of PKA have distinct functions. The Rla isoform is predominant in growing cells while the Rlla isoform is predominant in differentiated cells (Cho et al. (1995) Critical Reviews in Oncology/Hematology, 21 :33-61 ). The importance of Rla regulation for the cell is indicated by the fact that Rla knockout mice are embryonically lethal (Amieux et al, (1997) J. Biol. Chem., 272:3993). In Rlla and RII,Q knockout mice, Rla is up-regulated and seems to compensate for loss of these isoforms (Amieux et al. (1997) J. Biol. Chem., 272:3993-3998). However, novel phenotypes result since the mice are lean and have an increased tolerance to alcohol (Cummings et al. (1996) Nature, 382:622-626); Thiele et al. (2000) J.
Neuroscience, 20:RC75:1-6). These observations suggest an implication for dynamic PKA isoform regulation in lipid metabolism, which is contemplated herein as being modulated by D-AKAP2.
Also provided herein are methods of disrupting the binding of an Ile/Val(646) isoform of D-AKAP2 corresponding to SEQ ID Nos:64 and 65 to the Rla subunit of PKA comprising contacting the Rla subunit with a peptide provided herein, such as set forth in Examples 5-9 or in the claims. Also provided is a method for modulating the amount of PKA-Rla bound to D-AKAP2 in a cell comprising changing the effective intracellular concentration of the Val(646) isoform of D-AKAP29 (corresponding to SEQ ID N0:65) in the cell.
The change can be where the intracellular concentration of the Val(646) isoform is increased, thereby increasing the amount of PKA-Rla bound to D-AKAP2; or where the intracellular concentration of the Val(646) isoform is decreased, thereby decreasing the amount of PKA-Rla bound to D-AKAP2.

_7_ Also provided herein is a method for altering the intracellular location of PKA in a cell comprising changing the effective intracellular concentration of the Val(646) isoform of D-AKAP2 (corresponding to SEQ ID N0:65) in the cell. The change can be where the intracellular concentration of the Val(646) isoform is increased, thereby increasing the amount of PKA localized to the mitochondria;
or where the intracellular concentration of the Val(646) isoform is decreased, thereby decreasing the amount of PKA localized to the mitochondria. Also provided is a method of treating a subject manifesting a disease or disorder of signal transduction wherein there is an increased mitochondrial localization of PKA, said method comprising administering a peptide provided herein, such as disclosed in Example 5-9 and in the claims, or peptidomimetic thereof.
Also provided is a method of increasing the longevity of a subject in need thereof, comprising identifying a subject having the 646(Val) isoform of D-AKAP2 therein, and treating said subject with an agent that disrupts the binding the 646(Val) isoform of D-AKAP2 to Rla subunit of PKA. The agent can be any peptide disclosed herein, such as in Examples 5-9 or any of the claims, or peptidomimetic thereof. Also provided are methods for altering the ratio of PKA-Rla/PKA-Rlla bound to D-AKAP2 in a cell comprising increasing the concentration of a Val(646) variant of D-AKAP2, corresponding to SEQ ID
NO:65, in a cell, thereby increasing the ratio of PKA-Rla PKA/PKA-Rlla bound to D-AKAP2 in a cell. Also provided is a method of decreasing the concentration of CAMP required to stimulate a CAMP mediated signalling pathway, comprising increasing the concentration of a Val(646) variant of D-AKAP2, corresponding to SEQ ID N0:65, in a cell.
Also provided are methods of screening for agents that decrease or disrupt the binding of a Val(646) variant of D-AKAP2 with Rla PKA, comprising combining a candidate agent with a cell comprising a nucleotide sequence which encodes a Val(646) variant D-AKAP protein corresponding to SEQ ID N0:65 or the complement thereof, operably linked to a promoter such that the nucleotide sequence is expressed as a D-AKAP2 protein in the cell; and determining the effect of the agent upon the localization of PKA to the mitochondria, wherein a _g_ decrease in localization to the mitochondria identifies an agent that decreases the binding of a Val(646) variant of D-AKAP2 with Rla PKA.
Provided herein are methods of screening for agents that decrease or disrupt the binding of a Val(646) variant of D-AKAP2 to an Rla subunit of PKA, comprising combining a candidate agent with an admixture comprising Rla and a D-AKAP2 peptide sequence that binds to Rla; and determining the effect of the agent upon the binding of the peptide to Rla and/or the localization of PKA to the mitochondria. The candidate agent is combined with the admixture in a cell-free system or intracellularly. The peptide sequence can be obtained from any one of the Examples or claims provided herein.
Also provided are methods for identifying a molecule that modulates the biological activity of an D-AKAP2 protein, comprising combining the candidate molecule with a cell comprising a nucleotide sequence encoding a D-AKAP2 mutein or portion thereof that retains a biological activity exhibited by a full length variant protein, operably linked to a promoter such that the nucleotide sequence is expressed as an D-AKAP2 mutein or portion thereof in the cell; and determining the effect of the molecule upon a biological activity of the D-AKAP2 mutein or portion thereof. The biological activity of the D-AKAP2 mutein or portion thereof can be determined by examining signal transduction in the cell. The biological activity can be the binding of D-AKAP2 protein or portion thereof to protein kinase A. Also, the biological activity of the D-AKAP2 protein or portion thereof can be determined by examining protein phosphorylation in the cell. The screening methods provided herein can be high-throughput.
BRIEF DESCRIPTION OF THE FIGURES
Figure 1 shows the results of incubating membranes containing peptide array of the single amino acid substitutions using L-amino acids set forth in Example 7.
Figure 2A and 2B shows the binding of AKB (dual) (~), AKB (RI) [Q9F, V21 W, M25F] (~ ), AKB (RII) [A13L] (~) and AKB (null) (~) peptides to full-length Rla (A) and Rlla (B). Each peptide was fluorescently labeled and incubated with the corresponding regulatory subunit for 1 hour in 10mM Hepes, 150mM NaCI, _g_ 3mM EDTA pH 7.4. Fluorescence anisotropy was used to monitor bound peptide.
DETAILED DESCRIPTION
A. Definitions Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of skill in the art to which the inventions) belong. All patents, patent applications, published applications and publications, Genbank sequences, websites and other published materials referred to throughout the entire disclosure herein, unless noted otherwise, are incorporated by reference in their entirety. Where reference is made to a URL or other such identifier or address, it understood that such identifiers can change and particular information on the Internet can come and go, but equivalent information can be found by searching the Internet.
Reference thereto evidences the availability and public dissemination of such information.
As used herein, the term "mutein" refers to a peptide variant having one, two or more amino acid residue substitutions compared to a reference polypeptide, which can be a naturally occurring peptide sequence. The phrase "one, two, or more" in the context of amino acid residue substitutions encompasses peptide variants provided herein having one or more amino acid substitutions, e.g., in SEQ ID NOs:1, 2, 55, 63, 64 or 65, including peptides having any combination of 2, 3, 4, 5, 6, 7, 8, 9, 10 or more of the amino acid residue substitutions set forth herein, such as those described above and in Examples 5-9. In addition to these combinations of variants, conservative substitutions can be added at residues that have not yet been modified or at already modified residues within the peptides.
As used herein, the phrase "modified binding" refers to either an increase or decrease in binding affinity to the respective control peptide. Control peptides used herein can be either one or both of the naturally occurring full length Ile/Va1646 isoforms of D-AICAP2. Control peptides used herein can also be either one or both of the Ile/Val 27-mer isoforms set forth in SEQ ID NOs:1 or 2;
or full length proteins, such as SEQ ID NOs:55, 64 or 65.

As used herein, the phrase "normal binding" refers to a substantially equivalent binding affinity of a mutein to the respective control peptide.
As used herein, the term "segment" refers to a contiguous portion of the reference polypeptide or nucleic acid. For example, a segment of amino acids 12-23 of SEQ ID N0:1 refers to the contiguous stretch of amino acids 12-23 of SEQ ID N0:1 . The phrase "at least amino acids 12-23 of SEQ ID NOs:1 or 2 up to amino acids 1-27 of SEO ID NOs:1 or 2" refers to multiple segments of different sizes ranging from amino acids 12-23 of SEQ ID NOs:1 or 2; amino acids 12-24 of SEQ ID NOs:1 or 2; amino acids 11-23 of SEQ ID NOs:1 or 2;
amino acids 10-25 of SEQ ID NOs:1 or 2; up to amino acids 1-27 of SEQ ID
NOs:1 or 2.
As used herein, the phrase "a regulatory subunit of PKA" refers to one of the subunits in of a PKA holoenzyme. Exemplary PKA subunits include Rla and Rlla subunits.
As used herein, the phrase "peptide exhibits a preferred or exclusive binding to PKA-Rla subunits relative to PKA-Rlla subunits", or grammatical variations thereof, refers to a peptide that either has a higher binding affinity for PKA-Rla subunits than for PKA-Rlla or has the ability to bind to PKA-Rla and substantially no ability to bind PKA-Rlla.
As used herein, the phrase "peptide exhibits enhanced binding to both Rla and Rlla subunits" refers to a peptide that has a higher binding affinity for both PKA-Rla and PKA-Rlla subunits compared to the binding affinity of the peptide of SEQ ID NOs:1 or 2 for both PKA-Rla and PKA-Rlla subunits.
As used herein, the phrase "peptide exhibits a preferred or exclusive binding to PKA-Rlla subunits relative to PKA-Rla subunits", or grammatical variations thereof, refers to a peptide that either has a higher binding affinity for PKA-Rlla subunits than for PKA-Rla or has the ability to bind to PKA-Rlla and substantially no ability to bind PKA-Rla.
As used herein, "polymorphism" refers to the coexistence of more than one form of a gene or portion thereof. A portion of a gene of which there are at least two different forms, i.e., two different nucleotide sequences, is referred to as a "polymorphic region of a gene". A polymorphic region can be a single nucleotide, the identity of which differs in different alleles. A polymorphic region can also be several nucleotides in length.
As used herein, "polymorphic gene" refers to a gene having at least one polymorphic region.
As used herein, "allele", which is used interchangeably herein with "allelic variant", refers to alternative forms of a gene or portions thereof. Alleles occupy the same locus or position on homologous chromosomes. When a subject has two identical alleles of a gene, the subject is said to be homozygous for the gene or allele. When a subject has two different alleles of a gene, the subject is said to be heterozygous for the gene. Alleles of a specific gene can differ from each other in a single nucleotide, or several nucleotides, and can include substitutions, deletions, and insertions of nucleotides. An allele of a gene can also be a form of a gene containing a mutation.
As used herein, "predominant allele" refers to an allele that is represented in the greatest frequency for a given population. The allele or alleles that are present in lesser frequency are referred to as allelic variants.
As used herein, "associated" refers to coincidence with the development or manifestation of a disease, condition or phenotype. Association may be due to, but is not limited to, genes responsible for housekeeping functions whose alteration can provide the foundation for a variety of diseases and conditions, those that are part of a pathway that is involved in a specific disease, condition or phenotype and those that indirectly contribute to the manifestation of a disease, condition or phenotype.
As used herein, the term "subject" refers to mammals and in particular human beings.
As used herein, the term "gene" or "recombinant gene" refers to a nucleic acid molecule comprising an open reading frame and including at least one exon and (optionally) an intron sequence. A gene can be either RNA or DNA. Genes may include regions preceding and following the coding region (leader and trailer).
As used herein, "intron" refers to a DNA sequence present in a given gene which is spliced out during mRNA maturation.

As used herein, the term "coding sequence" refers to that portion of a gene that encodes an amino acid sequence of a protein.
As used herein, the amino acids, which occur in the various amino acid sequences appearing herein, are identified according to their well-known, three-s letter or one-letter abbreviations. The nucleotides, which occur in the various DNA fragments, are designated with the standard single-letter designations used routinely in the art Csee, Table 1 ).
As used herein, "amino acid residue" refers to an amino acid formed upon chemical digestion (hydrolysis) of a polypeptide at its peptide linkages. The amino acid residues described herein are preferably in the "L" isomeric form.
However, residues in the "D" isomeric form can be substituted for any L-amino acid residue, as long as the desired functional property is retained by the polypeptide. NHz refers to the free amino group present at the amino terminus of a polypeptide. COOH refers to the free carboxy group present at the carboxyl terminus of a polypeptide. In keeping with standard polypeptide nomenclature described in J. Biol. Chem., 243:3552-3559 (1969) and adopted at 37 C.F.R. ~
~ 1 .821 - 1.822, abbreviations for amino acid residues are shown in the following Table:

Table of Correspondence SYMBOL

1-Letter 3-Letter AMINO ACID

Y Tyr tyrosine G Gly glycine F Phe phenylalanine M Met methionine A Ala alanine S Ser serine I Ile isoleucine L Leu leucine SYM BOL

T Thr threonine V Val valine P Pro proline K Lys lysine H His histidine Q Gln glutamine E Glu glutamic acid Z Glx Glu and/or Gln W Trp tryptophan R Arg arginine D Asp aspartic acid N Asn asparagine B Asx Asn and/or Asp C Cys cysteine X Xaa Unknown or other It should be noted that all amino acid residue sequences represented herein by formulae have a left to right orientation in the conventional direction of amino-terminus to carboxyl-terminus. In addition, the phrase "amino acid residue" is broadly defined to include the amino acids listed in the Table of Correspondence and modified and unusual amino acids, such as those referred to in 37 C.F.R. ~ ~ 1.821-1.822, and incorporated herein by reference.
Furthermore, it should be noted that a dash at the beginning or end of an amino acid residue sequence indicates a peptide bond to a further sequence of one or more amino acid residues or to an amino-terminal group such as NHS or to a carboxyl-terminal group such as COOH.
In a peptide or protein, suitable conservative substitutions of amino acids are known to those of skill in this art and may be made generally without altering the biological activity of the resulting molecule. Those of skill in this art recognize that, in general, single amino acid substitutions in non-essential regions of a polypeptide do not substantially alter biological activity (see, e.g., Watson et al. (1987) Molecular Biology of the Gene, 4th Edition, The Benjamin/Cummings Pub. Co., p.224).
Such substitutions are preferably made in accordance with those set forth in TABLE 2 as follows:

Ala (A) Gly; Ser Arg (R) Lys Asn (N) Gln; His Cys (C) Ser Gln (Q) Asn Glu (E) Asp Gly (G) Ala; Pro His (H) Asn; Gln Ile (I) Leu; Val Leu (L) Ile; Val Lys (IC) Arg; Gln; Glu Met (M) Leu; Tyr; Ile Phe (F) Met; Leu; Tyr Ser (S) Thr Thr (T) Ser Trp (W) Tyr Tyr (Y) Trp; Phe Val (V) Ile; Leu Other substitutions are also permissible and may be determined empirically or in accord with known conservative substitutions.
As used herein, a DNA or nucleic acid homolog refers to a nucleic acid that includes a preselected conserved nucleotide sequence, such as a sequence encoding a therapeutic polypeptide. By the term "substantially homologous" is meant having at least 80%, preferably at least 90%, most preferably at least 95 % homology therewith or a less percentage of homology or identity and conserved biological activity or function.
The terms "homology" and "identity" are often used interchangeably. In this regard, percent homology or identity may be determined, for example, by comparing sequence information using a GAP computer program. The GAP
program uses the alignment method of Needleman and Wunsch (1970) J. Mo/.
Biol,, 48:443, as revised by Smith and Waterman (1981 ) Adv. App/. Math., 2:482. Briefly, the GAP program defines similarity as the number of aligned symbols (i.e., nucleotides or amino acids) which are similar, divided by the total number of symbols in the shorter of the two sequences. The preferred default parameters for the GAP program may include: (1 ) a unary comparison matrix (containing a value of 1 for identities and 0 for non-identities) and the weighted comparison matrix of Gribskov and Burgess (1986) Nucl. Acids Res., 14:6745, as described by Schwartz and Dayhoff, eds., ATLAS OF PROTEIN SEQUENCE
AND STRUCTURE, National Biomedical Research Foundation, pp. 353-358 (1979); (2) a penalty of 3.0 for each gap and an additional 0.10 penalty for each symbol in each gap; and (3) no penalty for end gaps.
Whether any two nucleic acid molecules have nucleotide sequences that are at least 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% "identical" can be determined using known computer algorithms such as the "FAST A" program, using for example, the default parameters as in Pearson and Lipman (1988) Proc.
Nat/. Acad. Sci. USA, 85:2444. Alternatively the BLAST function of the National Center for Biotechnology Information database may be used to determine identity In general, sequences are aligned so that the highest order match is obtained. "Identity" per se has an art-recognized meaning and can be calculated using published techniques. (See, e.g., Computational Molecular Bioloay, Lesk, A.M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D.W., ed., Academic Press, New York, 1993;
Computer Analysis of Seauence Data, Part I, Griffin, A.M., and Griffin, H.G., eds., Humana Press, New Jersey, 1994; Seauence Analysis in Molecular Biolo , yon Heinje, G., Academic Press, 1987; and Seauence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991 ).
While there exist a number of methods to measure identity between two polynucleotide or polypeptide sequences, the term "identity" is well known to skilled artisans (Carillo, H. & Lipton, D., (1988) SIAM J. Applied Math,, 48:1073. Methods commonly employed to determine identity or similarity between two sequences include, but are not limited to, those disclosed in Guide to Huae Computers, Martin J. Bishop, ed., Academic Press, San Diego, 1994;
and Carillo, H. & Lipton, D., (1988) SIAM J. Applied Math., 48:1073. Methods to determine identity and similarity are codified in computer programs.
Preferred computer program methods to determine identity and similarity between two sequences include, but are not limited to, GCG program package (Devereux, J.
et al. (1984) Nucleic Acids Research, 1211):387), BLASTP, BLASTN, FASTA
(Atschul, S.F. et al. (1990) J. Mol. Biol., 215:403).
Therefore, as used herein, the term "identity" represents a comparison between a test and a reference polypeptide or polynucleotide. For example, a test polypeptide may be defined as any polypeptide that is 90% or more identical to a reference polypeptide.
As used herein, the term at least "90% identical to" refers to percent identities from 90 to 99.99 relative to the reference polypeptides. Identity at a level of 90% or more is indicative of the fact that, assuming for exemplification purposes a test and reference polynucleotide length of 100 amino acids are compared, no more than 10% (i.e., 10 out of 100) amino acids in the test polypeptide differs from that of the reference polypeptides. Similar comparisons may be made between a test and reference polynucleotides. Such differences may be represented as point mutations randomly distributed over the entire length of an amino acid sequence or they may be clustered in one or more locations of varying length up to the maximum allowable, e.g., 10/100 amino acid difference (approximately 90% identity). Differences are defined as nucleic acid or amino acid substitutions, or deletions.
As used herein, stringency conditions refer to the washing conditions for removing the non-specific probes and conditions that are equivalent to either high, medium, or low stringency as described below:
1 ) high stringency: 0.1 x SSPE, 0.1 % SDS, 65°C
2) medium stringency: 0.2 x SSPE, 0.1 % SDS, 50°C
3) low stringency: 1 .0 x SSPE, 0.1 % SDS, 50°C.

It is understood that equivalent stringencies may be achieved using alternative buffers, salts and temperatures.
As used herein, "heterologous DNA" is DNA that encodes RNA and proteins that are not normally produced in vivo by the cell in which it is expressed or that mediates or encodes mediators that alter expression of endogenous DNA by affecting transcription, translation, or other regulatable biochemical processes or is not present in the exact orientation or position as the homologous DNA in a wildtype cell. Heterologous DNA may also be referred to as foreign DNA. Any DNA that one of skill in the art would recognize or consider as heterologous or foreign to the cell in which is expressed is herein encompassed by heterologous DNA. Examples of heterologous DNA include, but are not limited to, DNA that encodes traceable marker proteins, such as a protein that confers drug resistance, DNA that encodes therapeutically effective substances, such as anti-cancer agents, enzymes and hormones, and DNA that encodes other types of proteins, such as antibodies. Antibodies that are encoded by heterologous DNA may be secreted or expressed on the surface of the cell in which the heterologous DNA has been introduced.
As used herein, "isolated" in reference to a nucleic acid molecule or polypeptide or other biomolecule means that the nucleic acid or polypeptide has been separated from the genetic environment from which the polypeptide or nucleic acid was obtained. It may also mean altered from the natural state.
For example, a polynucleotide or a polypeptide naturally present in a living animal is not "isolated," but the same polynucleotide or polypeptide separated from the coexisting materials of its natural state is "isolated", as the term is employed herein. Thus, a polypeptide or polynucleotide produced and/or contained within a recombinant host cell is considered isolated. Also intended as an "isolated polypeptide" or an "isolated polynucleotide" are polypeptides or polynucleotides that have been purified, partially or substantially, from a recombinant host cell or from a native source. For example, a recombinantly produced version of a compounds can be substantially purified by the one-step method described in Smith and Johnson ( 1988) Gene, 67:31-40. The terms isolated and purified are sometimes used interchangeably.

Thus, by "isolated" is meant that the nucleic acid is free of the coding sequences of those genes that, in the naturally-occurring genome of the organism (if any) immediately flank the gene encoding the nucleic acid of interest. Isolated DNA may be single-stranded or double-stranded, and may be genomic DNA, cDNA, recombinant hybrid DNA, or synthetic DNA. It may be identical to a native DNA sequence, or may differ from such sequence by the deletion, addition, or substitution of one or more nucleotides.
Isolated or purified, as it refers to preparations made from biological cells or hosts, means any cell extract containing the indicated DNA or protein, including a crude extract of the DNA or protein of interest. For example, in the case of a protein, a purified preparation can be obtained following an individual technique or a series of preparative or biochemical techniques and the protein of interest can be present at various degrees of purity in these preparations.
The procedures may include for example, but are not limited to, ammonium sulfate fractionation, gel filtrbtion, ion exchange change chromatography, affinity chromatography, density gradient centrifugation and electrophoresis.
As used herein, "production by recombinant means by using recombinant DNA methods" refers to the use of the well known methods of molecular biology for expressing proteins encoded by cloned DNA, and may include methods such as gene shuffling and phage display with screening for desired specificities.
As used herein, a composition refers to any mixture of two or more products or compounds. It may be a solution, a suspension, liquid, powder, a paste, aqueous, non-aqueous or any combination thereof.
As used herein, a combination refers to any association between two or more items.
As used herein, "substantially identical to a product" means sufficiently similar so that the property of interest is sufficiently unchanged so that the substantially identical product can be used in place of the product.
As used herein, the term "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of preferred vector is an episome, i.e., a nucleic acid capable of extra-chromosomal replication. Preferred vectors are those capable of autonomous replication and/or expression of nucleic acids to which they are linked. Vectors capable of directing the expression of genes to which they are operatively linked are referred to herein as "expression vectors". In general, expression vectors of utility in recombinant DNA techniques are often in the form of "plasmids" which refer generally to circular double stranded DNA loops which, in their vector form are not bound to the chromosome. "Plasmid" and "vector" are used interchangeably as the plasmid is the most commonly used form of vector. Other such forms of expression vectors that serve equivalent functions and that become known in the art subsequently may be used.
As used herein, "predisposition to develop a disease or disorder" means that a subject having a particular genotype and/or haplotype has a higher likelihood than one not having such a genotype and/or haplotype for developing a particular disease or disorder.
As used herein, "morbidity" refers to conditions, such as diseases or disorders, that compromise the health and well-being of an organism, such as an animal. Morbidity susceptibility or morbidity-associated genes are genes that, when altered, for example, by a variation in nucleotide sequence, facilitate the expression of a specific disease clinical phenotype. Thus, morbidity susceptibility genes have the potential, upon alteration, of increasing the likelihood or general risk that an organism will develop a specific disease, which may decrease the longevity of the organism.
As used herein, "mortality" refers to the statistical likelihood that an organism, particularly an animal, will not survive a full predicted lifespan.
Hence, a trait or a marker, such as a polymorphism, associated with increased mortality is observed at a lower frequency in older than younger segments of a population.
As used herein, the phrase "increasing the longevity of a subject in need thereof" refers to prolonging the life and/or health of a subject or organism.
The methods provided herein are contemplated to increase the longevity or improve the health of a subject or organism by the administration of agents that disrupt the binding the 646(Val) isoform of D-AICAP2 to Rla subunit of PICA. For example, protein phosphorylation is an important mechanism for enzyme regulation and signal transduction in eukaryotic cells, and therefore is an importan mechanism to the longevity of cells and organisms as a whole. cAMP
dependent protein kinsae (PKA) mediates a variety of hormonal and neurotransmitter responses by phospyhorylating a wide variety of substrates including enzymes, membrane receptors, ion channels and transcription factors.
AKAPs direct the subcellular localization of cAMP-dependent protein kinase by binding to its regulatory subunits and therefore plays role in G-protein mediated receptor-signalling pathways (see, e.g., Huang et al. (1997) Proc. Nat/. Acad, Sci., USA 94:1 1 184). As set forth herein, AKAPs, such as D-AKAP2, have a PKA binding regions therein.
In addition, the main way to rapidly regulate contractility in the mammalian heart is through the a-adrenergic receptor (/3-AR) pathway. This mechanism is important as a means of responding to neurotransmitter (norepinephrine) or hormone (epinephrine) release. ~3-ARs belong to the large family of G protein-coupled receptors characterized by a typical structure with seven transmembrane domains. These receptors contain phosphorylation sites, which serve as targets for protein kinase A (PKA), protein kinase C, and a-adrenergic receptor kinases to desensitize the receptor in order to prevent an excessive ,l3-adrenergic stimulation. The combination of the a-receptor, the G-protein complex, and adenylyl cyclase is termed the /3-adrenergic system that enhances activity of adenylate cyclase increasing cAMP levels. cAMP then activates PKA which initiates a cascade of events eventually leading to an increase in heart rate and contractility. Therefore, PKA, and thus D-AKAP2, are involved in both the signal transduction after a-adrenergic stimulation and the receptor desensitization through its phosphorylation.
The D-AKAP2 variants at amino acid 646 of SEQ ID NOs:64 and 65 described herein map to the conserved AKB domain of D-AKAP2, which was previously shown to interact with the regulatory subunit of PKA. In accordance with the methods provided herein, it has been demonstrated that this variation in D-AKAP2 impacts the binding to PKA in an isoform specific manner both in vitro and in vivo. The Val(646) variant at amino acid 646 of SEQ ID N0:65, which has previously been identified as a deleterious allele associated with morbidity in the age-stratified approach (see, e.g., US Patent Application US20020040130A1 and PCT WO 02/04489), binds three-fold tighter to the Rla isoform when compared to the I1e1646) variant. At the cellular level, this affinity difference resulted in a decrease in mitochondria) localization of the Ile (646) variant. Accordingly, agents that disrupt the binding the 6461Va1) isoform of D-AKAP2 to Rla subunit of PKA are contemplated herein as having the ability to increase the longevity of a particular subject or organism.
As used herein, "transgenic animal" refers to any animal, preferably a non-human animal, e.g., a mammal, bird or an amphibian, in which one or more of the cells of the animal contain heterologous nucleic acid introduced by way of human intervention, such as by transgenic techniques well known in the art.
The nucleic acid is introduced into the cell, directly or indirectly by introduction into a precursor of the cell, by way of deliberate genetic manipulation, such as by microinjection or by infection with a recombinant virus. The term genetic manipulation does not include classical cross-breeding, or in vitro fertilization, but rather is directed to the introduction of a recombinant DNA molecule. This molecule may be integrated within a chromosome, or it may be extrachromosomally replicating DNA. In the typical transgenic animals described herein, the transgene causes cells to express a recombinant form of a protein.
However, transgenic animals in which the recombinant gene is silent are also contemplated, as for example, using the FLP or CRE recombinase dependent constructs. Moreover, "transgenic animal" also includes those recombinant animals in which gene disruption of one or more genes is caused by human intervention, including recombination and antisense techniques.
As used herein, "signal moiety" refers to any moiety that allows for the detection of a nucleic acid molecule. Included are moieties covalently attached to nucleic acids and those that are not.
As used herein, "molecule that modulates or effects the biological activity of an D-AKAP2 protein" refers to any drug, small molecule, nucleic acid (sense and antisense), ribozyme, protein, peptide, lipid, carbohydrate etc., or combination thereof, that directly or indirectly changes, alters, abolishes, increases or decreases a biological activity attributed to D-AKAP2 protein.

As used herein, "biological activity of an D-AKAP2 protein" refers to, but is not limited to: binding of D-AKAP2 to protein kinase A or its subunits (such as RI-a and/or RII-a); localization of D-AKAP2 protein to a subcellular site, e.g., the mitochondria; localization of protein kinase A to the mitochondria and/or binding of D-AKAP2 protein to other proteins including other signalling enzymes.
As used herein, "combining" refers to contacting the biologically active agent with a cell or animal such that the agent is introduced into the cell or animal. For a cell, any method that results in an agent traversing the plasma membrane is useful. For an animal, any of the standard routes of administration of an agent, e.g., oral, rectal, transmucosal, intestinal, intravenous, intraperitoneal, intraventricular, subcutaneous, intramuscular, etc., can be used.
As used herein, "solid support" refers to a support substrate or matrix, such as silica, polymeric materials or glass. At least one surface of the support can be partially planar. Regions of the support may be physically separated, for example with trenches, grooves, wells or the like. Some examples of solid supports include slides and beads. Supports are of such composition so as to allow for the immobilization or attachment of nucleic acids and other molecules such that these molecules retain their binding activity and/or biological activity .
As used herein, "array" refers to a collection of elements, such as nucleic acids, containing three or more members. An addressable array is one in which the members of the array are identifiable, typically by position on a solid support.
Hence, in general, the members of the array will be immobilized to discrete identifiable loci on the surface of a solid phase.
As used herein, "specifically hybridizes" refers to hybridization of a probe or primer preferentially to a target sequence versus a non-target sequence.
Those of skill in the art are familiar with parameters that affect hybridization;
such as temperature, probe or primer length and composition, buffer composition and salt concentration and can readily adjust these parameters to achieve specific hybridization of a nucleic acid to a target sequence.
As used herein "nucleic acid" refers to polynucleotides such as deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The term should also be understood to include, as equivalents, derivatives, variants and analogs of either RNA or DNA made from nucleotide analogs, single-stranded (sense or antisense) and double-stranded polynucleotides. Deoxyribonucleotides include deoxyadenosine, deoxycytidine, deoxyguanosine and deoxythymidine. For RNA, the uracil base is uridine.
As used herein, "at a position corresponding to" refers to a position of interest (i.e., base number or residue number) in a nucleic acid molecule or protein relative to the position in another reference nucleic acid molecule or protein. Corresponding positions can be determined by comparing and aligning sequences to maximize the number of matching nucleotides or residues, for example, such that identity between the sequences is greater than 95 %, preferably greater than 96%, more preferably greater than 97%, even more preferably greater than 98% and most preferably greater than 99%. The position of interest is then given the number assigned in the reference nucleic acid molecule. For example, it is shown herein that a particular polymorphism in D-AICAP2 occurs at nucleotide 2073 of SEQ ID N0:63. To identify the corresponding nucleotide in another allele or isolate, the sequences are aligned and then the position that lines up with 2073 is identified. Since various alleles may be of different length, the position designate 2073 may not be nucleotide 2073, but instead is at a position that "corresponds" to the position in the reference sequence.
As used herein, "primer" and "probe" refer to a nucleic acid molecule including DNA, RNA and analogs thereof, including protein nucleic acids (PNA), and mixtures thereof. Such molecules are typically of a length such that they are statistically unique (i.e., occur only once) in the genome of interest.
Generally, for a probe or primer to be unique in the human genome, it contains at least 14, 16 or contiguous nucleotides of a sequence complementary to or identical to a gene of interest. Probes and primers can be 10, 20, 30, 50, 100 or more nucleic acids long.
As used herein, "antisense nucleic acid molecule" refers to a molecule encoding a sequence complementary to at least a portion of an RNA molecule.
The sequence is sufficiently complementary to be able to hybridize with the RNA, preferably under moderate or high stringency conditions to form a stable duplex. The ability to hybridize depends on the degree of complementarity and the length of the antisense nucleic acid. Generally, the longer the hybridizing nucleic acid, the more base mismatches with an RNA it can contain and still form a stable duplex. One skilled in the art can ascertain a tolerable degree of mismatch by use of standard procedures to determine the melting point of the hybridized complex.
As used herein, a "variant protein" or "variant peptide" refers to a protein or peptide encoded by a mutein variant of a D-AKAP2 gene which results in a change of an amino acid residue at a particular position relative to that position in the protein encoded by the predominant allele. Accordingly, a mutein protein or peptide refers to a polypeptide sequence that differs or varies from a respective naturally occurring sequence by one, two or more amino acid residues. For example, in the peptides provided herein, combinations of 2, 3, 4, 5, 6, 7, 8, 9, 10 or more residue substitutions compared to a particular reference sequence, such as SEQ ID NOs:1 or 2, are contemplated herein in a single peptide. In a particular embodiment, the residue substitutions can be any combination of one or more of the single amino acid substitutions set forth herein, such as in Examples 5-9. In addition, other amino acid residue substitutions can be added to those disclosed herein, both conservative and non-conservative.
As used herein, "signal transduction" refers to the propagation of a signal. In general, an extracellular signal is transmitted through the cell membrane to become an intracellular signal. This signal can then stimulate a cellular response. The term also encompasses signals that are propagated entirely within a cell. The polypeptide molecules involved in signal transduction processes are typically receptor and non-receptor protein kinases, receptor and non-receptor protein phosphatases, nucleotide exchange factors, transcription factors, G-coupled protein receptors, G-proteins, and GTPase regulators. One of the key biochemical mechanisms involved in signal transduction is protein phosphorylation. D-AKAP2 proteins are involved in signal transduction as they bind to protein kinase A (PKA) and are thought to anchor the kinase at a location, e.g., the mitochondria, where PKA acts to phosphorylate a specific substrate, either at the mitochondria or at an ion channel. Thus, an alteration in D-AKAP2 binding to PKA, localization to the mitochondria, or phosphorylation by PKA, among other steps, will result in an alteration in signal transduction.
Assays including those that determine phosphorylation by PKA, association of PKA and D-AKAP2 and localization of D-AKAP2 can be used to monitor the state of signal transduction.
As used herein, "binding to PKA", refers to the interaction of the PKA
binding domain (also referred to herein as the A-Kinase binding (AKB) domain) of an D-AKAP2 protein and the regulatory subunits RI and/or RII of the protein kinase A holoenzyme. For example, the AKB domain of human D-AKAP2 corresponds to amino acids 623-649 of SEQ ID NOs:63-65.
B. Polymorphic AKAPs Polymorphisms of the genome can lead to altered gene function, protein function or mRNA instability. AKAPs provide a mechanism for regulating ubiquitous cAMP-dependent kinase (PKA) activity by tethering PKA to specific subcellular locations thereby segregating it with particular components in a given signaling pathway and contributing to specificity in cellular responses to extracellular signals. AKAPs thus play a fundamental role in the basic functioning of cells, the response of cells to their environment and ultimately in the coordination of vital systems within an organism. Therefore, polymorphisms in AKAP gene sequences may significantly affect the proper functioning of cells and systems within organisms and could be directly linked with certain disorders or could predispose an organism to a variety of diseases and disorders, especially those involving alterations in cellular protein phosphorylation and/or signal transduction. Among such disorders and diseases are: neurodegenerative diseases, such as Alzheimer's Disease, cardiovascular disorders, cardiac disorders, particularly disorders associated with altered left ventricular function, cardiomyopathies, proliferative disorders, bipolar disorder and other neurological disorders, obesity, neoplastic disease, diabetes, certain peripheral retinopathies, such as retinitis pigmentosa, and autoimmune disorders, such as Lupus erythematosus. The discovery of AKAP gene polymorphisms, such as those described herein, provides for the identification and development of diagnostic and prognostic methods, also provided herein, and the development of drug therapies and treatment regimens. Furthermore, polymorphisms of AKAP genes aid in the study of AKAP protein structure and function, which also contributes to the development of diagnostic methods and therapies.
1. D-AKAP2 Although the mechanisms for targeting of D-AKAP2 are not known, the D-AKAP2 protein can be found associated with mitochondria. The sequence of a human D-AKAP2 cDNA (also referred to as D-AKAP2) is available in the GenBank database, at accession numbers AF037439 and NM 007202, and is provided in SEQ ID N0:63. The D-AKAP2 gene is located on chromosome 17.
The sequence of a mouse D-AKAP2 cDNA is also available in the GenBank database (see accession number AF021833). The mouse D-AKAP2 protein contains two RGS domains near the amino terminus that is characteristic of proteins that interact with Ga subunits and possess GTPase activating protein-like activity (Huang et al. (1997) PNAS, USA, 94:1 1 184-1 1 189; and Wang et al. (2001 ) PNAS, USA, 98 6 :3220-3225). The human D-AKAP2 protein also has sequences homologous to two RGS domains. The carboxy-terminal 40 residues of the mouse D-AKAP2 protein are responsible for the interaction with the regulatory subunits of PKA. This sequence is fairly well conserved between the mouse D-AKAP2 and human D-AKAP2 proteins.
2. Polymorphisms of the human D-AKAP2 gene and polymorphic D-AKAP2 proteins Polymorphisms of AKAP genes that alter gene expression, regulation, protein structure and/or protein function are more likely to have a significant effect on the regulation of enzyme (particularly PKA) activity, cellular transduction of signals and responses thereto and on the basic functioning of cells than polymorphisms that do not alter gene and/or protein function.
Included in the polymorphic AKAPs provided herein are human D-AKAP2 proteins containing differing amino acid residues at position number 646 of SEQ
ID N0:64.

Amino acid 646 of the human D-AKAP2 protein (SEQ ID N0:64) is located in the carboxy-terminal region of the protein within a segment that participates in the binding of R-subunits of PKAs. This segment includes the carboxy-terminal 40 amino acids.
The amino acid residue reported for position 646 of the human D-AKAP2 protein is an isoleucine. However, an allelic variant of the human D-AKAP2 gene is at the polymorphic site at position 2073 of the coding sequence (see SEO.
ID
N0:63) and encodes a valine at position 646 of the D-AKAP2 protein. This allelic variant has been found to vary in frequency in DNA samples from younger and older segments of a healthy population. This allele has the A at position 2073 of the D-AKAP2 gene coding sequence of SEQ ID N0:63 changed to a G.
Consequently, the codon for amino acid 646 changes from ATT, coding for isoleucine, to GTT, coding for valine, as set forth in SEQ ID N0:65.
C. Peptides, Polypeptides and Peptide Mimetics Provided herein are D-AKAP2 mutein proteins, mutein peptides thereof, and methods for identifying molecules (agents) that bind to and modulate the activity of PKA proteins. Included among muteins that bind to PKAs, particularly the regulatory subunits Rla and Rlla, are peptides derived from the AKB
binding domain of the Ile/Val(646) variants of human D-AKAP2 corresponding to amino acids 623-649 of SEQ ID NOs:64 and 65; polypeptides and peptide mimetics thereof, including cyclic peptides. In one embodiment, exemplary D-AKAP2-derived peptides provided herein comprise between 12 and 39 amino acid residues, wherein the peptide comprises amino acids 7-21 of SEQ ID NOs:1 or 2.
In another embodiment, exemplary peptides comprise between 12 and 39 amino acid residues, wherein the peptide comprises a segment of SEQ ID NOs:1 or 2, wherein the length of the segment ranges from at least amino acids 12-23 of SEQ ID NOs:1 or 2, up to amino acids 1-27 of SEQ ID NOs:1 or 2. Accordingly, peptides having a length of 13, 14, 15, 16, 17, 18, 19, 20, 21 , 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, or 39 amino acids are provided herein. In another embodiment, exemplary D-AKAP2-derived peptides provided herein comprise between 15 and 39 amino acid residues, wherein the peptide comprises amino acids 7-21 of SEQ ID NOs:1 or 2. In one embodiment, the peptide binds to a regulatory subunit of PKA. In one embodiment the peptide binds to Rlla. In another embodiment, the peptide comprises at least amino acids 1 1-24 of SEQ ID NOs:1 or 2 and binds to Rla. In a particular embodiment, the peptides provided herein bind to both Rla and Rlla subunits.
In one embodiment, the peptide exhibits a preferred or exclusive binding to PKA-Rla subunits relative to PKA-Rlla subunits; or enhanced binding to both Rla and Rlla subunits. In this particular embodiment, the peptide can be selected from the group consisting of peptides that correspond to the substitution in SEQ
ID NOs:1 or 2 of one, two or more: of Q at residue 9 with F, I, L, V, H, M, R, T, W or Y; of L at residue 12 with F, W or Y; of V at residue 21 with I, L or W;
and of M at residue 25 with F, I, L, T, V, W or Y. In a particular embodiment, the peptide is 27 amino acids in length. In another embodiment, V at residue 21 is substituted with W. In yet another embodiment, V at residue 21 is substituted with W, and the peptide further comprises the substitution of either one or both of Q at residue 9 with F, and of M at residue 25 with F.
In another embodiment, the peptide exhibits a preferred or exclusive binding to PKA-Rlla subunits relative to PKA-Rla subunits. In this particular embodiment, the peptide can be selected from the group consisting of peptides that correspond to the substitution in SEQ ID NO:2 of one, two or more: of L
at residue 12 with A, C, or K; of A at residue 13 with F, H, I, K, L, M or N; of W at residue 14 with C; of K at residue 15 with C; of K at residue 18 with C; of M
at residue 19 with C; of S at residue 22 with C; and of D at residue 23 with C.
In a particular embodiment, the peptide is 27 amino acids in length.
In yet another embodiment, the peptide is 27 amino acids in length and exhibits preferred or exclusive binding to PKA-Rlla subunits relative to PKA-Rla subunits. In this particular embodiment, the peptide can be selected from the group consisting of peptides that correspond to the substitution in SEQ ID
N0:1 of one, two or more: of A at residue 13 with F, H, I, L, M and S; of W at residue 14 with C; of K at residue 15 with C; of K at residue 18 with C; of M at residue 19 with C; of S at residue 22 with C; and of D at residue 23 with C.
In another embodiment, the D-AKAP2-derived peptides have substantially no ability to bind to PKA-Rla subunit while maintaining the ability to bind to PKA-Rlla subunit, compared to the PKA R-subunit binding ability of the peptide of SEQ ID NOs:1 or 2. In this particular embodiment the peptide can be selected from the group consisting of:

VQGNTDEAQEELFWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:27) VQGNTDEAQEELIWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:28) VQGNTDEAQEELLWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:29) VQGNTDEAQEELAWCIAKMIVSD[I/V]MQQ; (SEQ ID N0:30) VQGNTDEAQEELAWKIACMIVSD[I/V]MQQ; (SEQ ID N0:31 ) VQGNTDEAQEELAWKIAKCIVSD[I/V]MQQ; (SEQ ID N0:32) and VQGNTDEAQEELAWKIAKMIVCD[I/V]MQQ. (SEQ ID N0:33) In another embodiment, the D-AKAP2-derived peptides have substantially no ability to bind to a PKA-Rla aintaining a reduced subunit while m ability to bind to a PKA-Rlla subunit, compared subunit binding ability to the PKA R- of the peptide of SEQ ID NOs:1 or 2. In r embodiment, the peptide this particula can be selected from the group consisting of:

VQGNTDEAQEECAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:34) VQGNTDEAQEEKAWKIAKMIVSDfI/V]MQQ; (SEQ ID N0:35) VQGNTDEAQEELHWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:36) VQGNTDEAQEELKWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:37) VQGNTDEAQEELMWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:38) VQGNTDEAQEELNWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:39) VQGNTDEAQEELVWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:40) VQGNTDEAQEELWWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:41 ) VQGNTDEAQEELYWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:42) VQGNTDEAQEELAWKIAKMIVSCfI/V]MQQ; (SEQ ID N0:43) TDEAQEELAWKIAKMIVSD; (SEQ ID N0:8) DEAQEELAWKIAKMIVS; and (SEQ ID N0:9) EAQEELAWKIAKMIV. (SEQ ID N0:4) In still a further embodiment, the D-AKAP2-derived peptide has enhanced binding to both PKA-Rla subunit and PKA Rlla subunit, compared to PKA R-subunit binding ability of the peptide of SEQ ID NOs:1 or 2. In this particular embodiment, the peptide can be selectedthe group consisting from of:

VQGNTDEAFEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:44) VQGNTDEAIEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:45) VQGNTDEALEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:46) VQGNTDEAVEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:47) VQGNTDEAQEELAWKIAKMIVSD[I/V]FQQ; (SEQ ID N0:48) VQGNTDEAQEELAWKIAKMIVSD[I/V]IQQ; (SEQ ID N0:49) and VQGNTDEAQEELAWKIAKMIVSD[I/V]LQQ. (SEQ ID N0:50) In yet another embodiment, the peptide that has enhanced ability to bind to PKA-Rla subunit, while maintaining a normal or reduced ability to bind to PKA-Rlla subunit, compared to the PKA R-subunit binding ability of the peptide of SEQ ID NOs:1 or 2. In this particular embodiment, the peptide can be selected from the group consisting of:
VQGNTDEAQEEFAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:51 ) VQGNTDEAQEELAWKIAKMIISD[I/V]MQQ; (SEQ ID N0:52) and VQGNTDEAQEELAWKIAKMILSD[I/V]MQQ. (SEQ ID N0:53) In yet a further embodiment, the D-AKAP peptide has an ability to bind to PKA-Rla subunit but substantially no ability to bind to PKA-Rlla subunit, compared to the PKA R-subunit binding ability of the peptide of SEQ ID NOs:1 or 2. In this particular embodiment, the peptide can comprise VQGNTDEAQEELAWKIAKMIWSD[I/V]MQQ (SEQ ID N0:54).
In yet a further embodiment, the D-AKAP peptide has a D-amino acid at the position indicated in bold, and an enhanced ability to bind to PKA-Rla subunit, and a reduced ability to bind to PKA-Rlla subunit, compared to the PKA
R-subunit binding ability of the peptide of SEQ ID NOs:1 or 2. In this particular embodiment, the peptide can be selected from the group consisting of:
VO.GNTTEAQEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:17) VQGNTDEAFEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:18) VQGNTDEAIEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:19) VQGNTDEALEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:20) VQGNTDEAVEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:21 ) VQGNTDEAWEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:22) VQGNTDEAYEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:23) VQGNTDEAQEELAWKIAKMILSD[I/V]MQQ; (SEQ ID N0:24) VQGNTDEAQEELAWKIAKMIVLD[I/V]MQQ; (SEQ ID N0:25) and VQGNTDEAQEELAWKIAKMIVSD[I/V]FQQ. (SEQ ID N0:26).

In another embodiment, the D-AKAP peptide has enhanced ability to bind to PKA-Rla subunit, and a reduced ability to bind to PKA-Rlla subunit, compared to the peptide of SEQ ID NOs:1 or 2, wherein the peptide is selected from the group consisting of:
FEELAWKIAKMIWSDVMQQC; (SEQ ID N0:104; PV-37) FEELAWKIAKMIWSDVFQQC; (SEQ ID N0:103; PV-38) QEEFAWKIAKMIVSDVFQQC; (SEQ ID N0:105; PV-47) QEEFAWKIAKMIISDVFQQC; (SEQ ID N0:106; PV-48).
In another embodiment, the peptide has enhanced ability to bind to PKA-Rla subunit, while maintaining a normal ability to bind to PKA-Rlla subunit, compared to the peptide of SEQ ID N0:1 or 2, wherein the peptide is:
FEELAWKIAKMIISDVFQQC (SEQ ID N0:107; PV-49).
In another embodiment, peptide variants provided herein having one or more amino acid substitutions in SEQ ID NOs:1 or 2, include peptides having any combination of 2, 3, 4, 5, 6, 7, 8, 9, 10 or more of the amino acid residue substitutions set forth herein, such as those described above and in Examples 9. In addition to these combinations of variants, conservative substitutions can be added at residues that have not yet been modified or at already modified residues within the peptides.
Also provided herein, based on the particular effect that a specific amino acid residue substitution has on the binding of a substituted peptide variant to either one or both of Rla or Rlla, one or more amino acid residue substitutions can be selected to either selectively increase or decrease (i.e., disrupt) the binding affinity, and thereby increase the selectivity of a particular peptide for either Rla or Rlla. The residues can be selected based on the effect the residue substitutions provided herein as set forth in Examples 5-9 and Figure 1 .
Accordingly, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more residues that enhance binding a peptide to either one or both of RI or RII subunits can be combined.
Accordingly, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more residues that decrease binding a peptide to either one or both of RI or RII subunits can be combined. In certain embodiments, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more residues that either increase or decrease binding a peptide to either one or both of RI or RII subunits can be combined (e.g., residues substituted to increase binding to one R subunit and residues substituted to decrease binding to the other R subunit), and the like.
Accordingly, combinations of two or more amino acid substitutions that increase binding affinity for Rla and/or decrease binding affinity for Rlla are contemplated herein. In another embodiment, combinations of two or more amino acid substitutions that decrease binding affinity for Rla and/or increase binding affinity for Rlla are contemplated herein.
For example, in one embodiment, peptides are provided having in a region corresponding to SEQ ID NOs:1 or 2, a combination of any 2 amino acid residue substitutions set forth herein. In another embodiment, peptides are provided having in a region corresponding to SEQ ID NOs:1 or 2, a combination of any 3 amino acid residue substitutions set forth herein. In another embodiment, peptides are provided having in a region corresponding to SEQ ID NOs:1 or 2, a combination of any 4 amino acid residue substitutions set forth herein. In another embodiment, peptides are provided having in a region corresponding to SEQ ID NOs:1 or 2, a combination of any 5 amino acid residue substitutions set forth herein. In another embodiment, peptides are provided having in a region corresponding to SEQ ID NOs:1 or 2, a combination of any 6 amino acid residue substitutions set forth herein. In another embodiment, peptides are provided having in a region corresponding to SEQ ID NOs:1 or 2, a combination of any 6 amino acid residue substitutions set forth herein. In another embodiment, peptides are provided having in a region corresponding to SEQ ID NOs:1 or 2, a combination of any 7 amino acid residue substitutions set forth herein. In another embodiment, peptides are provided having in a region corresponding to SEQ ID NOs:1 or 2, a combination of any 8 amino acid residue substitutions set forth herein. In another embodiment, peptides are provided having in a region corresponding to SEQ ID NOs:1 or 2, a combination of any 9 amino acid residue substitutions set forth herein. In another embodiment, peptides are provided having in a region corresponding to SEQ ID NOs:1 or 2, a combination of any 10 amino acid residue substitutions set forth herein.
Accordingly, provided herein are polypeptides that are muteins of a D-AKAP2 polypeptide, wherein the mutein exhibits modified binding to a regulatory subunit of PKA compared to a native D-AKAP2. The native D-AKAP2 can comprise a sequence of amino acids set forth as SEQ ID NOs:1 or 2. Also provided are polypeptides that are muteins of a D-AKAP2 polypeptide, wherein the mutein exhibits modified binding to a regulatory subunit of PKA compared to a control. The control is a polypeptide that consists essentially of the sequence of amino acids set forth as SEQ ID NOs:1 or 2. In one embodiment, the polypeptides exhibit enhanced binding to PKA-Rla subunits. These peptides can further exhibit normal or reduced binding to PKA-Rlla subunits.
In another embodiment, the polypeptides exhibit enhanced binding to PKA-Rlla subunits relative to PKA-Rla subunits. The peptides can further exhibit normal or reduced binding to PKA-Rla subunits. In another embodiment, the peptides exhibit enhanced binding to both Rla and Rlla subunits. In another embodiment, the peptides exhibit reduced binding to PKA-Rla subunits. These peptides can .further exhibit normal or increased binding to PKA-Rlla subunits. In another embodiment, the polypeptides exhibit reduced binding to PKA-Rlla. The peptides can further exhibit normal or increased binding to PKA-Rla subunits.
In yet another embodiment, the peptides exhibit reduced binding to both Rla and Rlla subunits.
Also provided herein are variant D-AKAP2 peptides comprising one, two or more amino acid residue substitutions at positions corresponding to 1-27 of SEQ ID NOs:1 or 2, wherein the peptide further comprises a range of one or more amino acids, up to all of the amino acids, selected from amino acids 1-and 650-662 of SEQ ID NOs:64 or 65, wherein the one or more selected amino acids are contiguous with amino acid positions corresponding to 623-649 of SEQ ID NOs:64 or 65. In other words, polypeptides are provided herein that are at least 28 amino acids in length up to 662 amino acids that comprise the region corresponding to 623-649 of SEQ ID NOs:64 or 65, wherein the region corresponding to 623-649 of SEQ ID NOs:64 or 65 contains any combination of one, two or more of the amino acid residue substitutions provided herein, such as in the Examples or in the claims. Accordingly, in addition to containing single amino acid substitutions, D-AKAP2 proteins are provided having in a region corresponding to SEQ ID NOs:1 or 2 (e.g., the region corresponding to 623-649 of SEQ ID NOs:64 or 651, a combination of any 2, 3, 4, 5, 6, 7, 8, 9, 10 or more amino acid residue substitutions set forth herein, such as in the Examples and in the claims.
In other embodiments, the additional amino acid residues obtained from amino acids 1-622 and 650-662 of SEQ ID NOs:64 or 65 can contain conservative substitutions therein that do not alter the desired effect obtained by substituting one or more residues in the peptide region corresponding to 623-649 of SEQ ID NOs:64 or 65. In addition, these polypeptides can further comprise additional contiguous amino acids at either end of the D-AKAP2 variant protein, where the additional amino acid sequence can be used for a variety of purposes, such as protein targeting, to facilitate protein purification.
Peptide mimetics are molecules or compounds that mimic the necessary molecular conformation of a ~ligand or polypeptide for specific binding to a target molecule such as a PKA holoenzyme: In an exemplary embodiment, the peptides, polypeptides or peptide mimetics bind to the RI and/or RII
regulatory subunits of the PKA holoenzyme. Such peptides and peptide mimetics include those of antibodies that specifically bind to a PKA holoenzyme and, typically, bind to the RI and/or RII regulatory subunits of a PKA holoenzyme. The peptides, polypeptides and peptide mimetics identified by methods provided herein can be agonists or antagonists of PKA holoenzymes.
Such peptides, polypeptides and peptide mimetics are useful for diagnosing, treating, preventing, and screening for a disease or disorder associated with PKA holoenzyme activity in a mammal. In addition, the peptides and peptide mimetics are useful for identifying, isolating, and purifying molecules or compounds that modulate the activity of a PKA holoenzyme, or specifically bind to a PKA holoenzyme, generally the RI and/or RII regulatory subunits of a PKA holoenzyme. Low molecular weight peptides and peptide mimetics can have strong binding properties to a target molecule, e.g., a PKA holoenzyme or the RI and/or RII regulatory subunits of a PKA holoenzyme.
Peptides, polypeptides and peptide mimetics that bind to PKA
holoenzymes as described herein can be administered to mammals, including humans, to modulate PKA holoenzyme activity. Thus, methods for therapeutic treatment and prevention of neurodegenerative diseases, such as Alzheimer's Disease, cardiovascular disorders, cardiac disorders, particularly disorders associated with altered left ventricular function, cardiomyopathies, proliferative disorders, bipolar disorder and other neurological disorders, lipid-metabolism disorders, such as obesity, neoplastic disease, diabetes and certain peripheral retinopathies, such as retinitis pigmentosa, and autoimmune disorders, such as Lupus erythematosus, comprise administering a peptide, polypeptide or peptide mimetic compound in an amount sufficient to modulate such activity are provided. Also provided herein are methods for treating a subject having such a disease or disorder in which a peptide, polypeptide or peptide mimetic compound is administered to the subject in a therapeutically effective dose or amount.
Compositions containing the peptides, polypeptides or peptide mimetics provided herein can be administered for prophylactic and/or therapeutic treatments. In therapeutic applications, compositions can be administered to a patient already suffering from a disease, as described above, in an therapeutically effective amount sufficient to cure or at least partially arrest the symptoms of the disease and its complications. Amounts effective for this use will depend on the severity of the disease and the weight and general state of the patient and can be empirically determined.
In prophylactic applications, compositions containing the peptides, polypeptides and peptide mimetics are administered to a patient susceptible to or otherwise at risk of a particular disease. Such an amount is defined to be a "prophylactically effective dose". In this use, the precise amounts again depend on the patient's state of health and weight. Accordingly, the peptides, polypeptides and peptide mimetics that bind to a PKA holoenzyme can be used to prepare pharmaceutical compositions containing, as an active ingredient, at least one of the peptides or peptide mimetics in association with a pharmaceutical carrier or diluent. The compounds can be administered, for example, by oral, pulmonary, parental (intramuscular, intraperitoneal, intravenous (IV) or subcutaneous injection), inhalation (via a fine powder formulation), transdermal, nasal, vaginal, rectal, or sublingual routes of administration and can be formulated in dosage forms appropriate for each route of administration (see, e.g., International PCT application Nos. W093/25221 and W094/17784; and European Patent Application 613,683).
Peptides, polypeptides and peptide mimetics that bind to PKA
holoenzymes are useful in vitro as unique tools for understanding the biological role of PKA holoenzymes, including the evaluation of the many factors thought to influence, and be influenced by, the production of PKA holoenzyme. Such peptides, polypeptides and peptide mimetics are also useful in the development of other compounds that bind to and modulate the activity of a PKA
holoenzyme, because such compounds provide important information on the relationship between structure and activity that should facilitate such development.
The peptides, polypeptides and peptide mimetics are also useful as competitive binders in assays to screen for new PKA holoenzymes or PKA
holoenzyme agonists. In such assay embodiments, the compounds can be used without modification or can be modified in a variety of ways; for example, by labeling, such as covalently or non-covalently joining a moiety which directly or indirectly provides a detectable signal. In any of these assays, the materials thereto can be labeled either directly or indirectly. Exemplary labels for direct labeling include label groups such as: radiolabels such as '251 enzymes (U.S.
Pat.
No. 3,645,090), peroxidase and alkaline phosphatase, and fluorescent labels (U.S. Pat. No. 3,940,475) capable of monitoring the change in fluorescence intensity, wavelength shift, or fluorescence polarization. Exemplary labels for indirect labeling include biotinylation of one constituent followed by binding to avidin coupled to one of the above label groups. The compounds can also include spacers or linkers in cases where the compounds are to be attached to a solid support.
In addition, based on their ability to bind to a PKA holoenzyme, the peptides, polypeptides and peptide mimetics can be used as reagents for detecting PKA holoenzymes in living cells, fixed cells, in biological fluids, in tissue homogenates and in purified, natural biological materials. For example, by labelling such peptides, polypeptides and peptide mimetics, cells having PKA
holoenzymes can be identified. In addition, based on their ability to bind a PKA
holoenzyme, the peptides, polypeptides and peptide mimetics can be used in in situ staining, FACS (fluorescence-activated cell sorting), Western blotting, ELISA
and other analytical protocols. Based on their ability to bind to a PKA
holoenzyme, the peptides, polypeptides and peptide mimetics can be used in purification of PKA holoenzymes or in purifying cells expressing the PKA
holoenzymes, e.g., a polypeptide encoding the RI and/or RII regulatory subunits of a PKA holoenzyme.
The peptides, polypeptides and peptide mimetics can also be used as commercial reagents for various medical research and diagnostic uses. The activity of the peptides and peptide mimetics can be evaluated either in vitro or in vivo in one of the numerous models described in McDonald (1992) Am. J. of Pediatric Hematology/Oncology, 14:8-21 .
D. Peptide, polypeptide and peptide mimetic therapy Peptide analogs are commonly used in the pharmaceutical industry as non-peptide drugs with properties analogous to those of the template peptide.
These types of non-peptide compounds are termed "peptide mimetics" or "peptidomimetics" (Luthman et al. A Textbook of Drug Design and Development, 14:386-406, 2nd Ed., Harwood Academic Publishers (1996); Joachim Grante ( 1994) Angew. Chem. lnt. Ed. Engl., 33:1699-1720; Fauchere ( 1986) J. Adv.
Drug Res., 15:29; Veber and Freidinger (1985) TINS, p. 392; and Evans et al.
(1987) J. Med. Chem., 30:1229). Peptide mimetics that are structurally similar to therapeutically useful D-AKAP2-derived peptides provided herein can be used to produce an equivalent or enhanced therapeutic or prophylactic effect.

Preparation of peptidomimetics and structures thereof are known to those of skill in this art.
Systematic substitution of one or more amino acids of a consensus sequence with a D-amino acid of the same type (e.g., D-lysine in place of L-lysine) can be used to generate more stable peptides. In addition, constrained peptides containing a consensus sequence or a substantially identical consensus sequence variation can be generated by methods known in the art (Rizo et al.
(1992) An. Rev. Biochem., 61 :387, incorporated herein by reference); for example, by adding internal cysteine residues capable of forming intramolecular disulfide bridges which cyclize the peptide.
Those skilled in the art appreciate that modifications can be made to the peptides and mimetics without deleteriously effecting the biological or functional activity of the peptide. Further, the skilled artisan would know how to design non-peptide structures in three dimensional terms, that mimic the peptides that bind to a target molecule, e.g., a PICA holoenzyme or, generally, the RI
and/or RII
subunit of PICA holoenzymes (see, e.g., Eck and Sprang (1989) J. Bioi. Chem., 26:17605-18795).
When used for diagnostic purposes, the peptides and peptide mimetics can be labeled with a detectable label and, accordingly, the peptides and peptide mimetics without such a label can serve as intermediates in the preparation of labeled peptides and peptide mimetics. Detectable labels can be molecules or compounds, which when covalently attached to the peptides and peptide mimetics, permit detection of the peptide and peptide mimetics in vivv, for example, in a patient to whom the peptide or peptide mimetic has been administered, or in vitro, e.g., in a sample or cells. Suitable detectable labels are well known in the art and include, by way of example, radioisotopes, fluorescent labels (e.g., fluorescein), and the like. The particular detectable label employed is not critical and is selected to be detectable at non-toxic levels.
Selection of the such labels is well within the skill of the art.
Covalent attachment of a detectable label to the peptide or peptide mimetic is accomplished by conventional methods well known in the art. For example, when the'Z51 radioisotope is employed as the detectable label, covalent attachment of X251 to the peptide or the peptide mimetic can be achieved by incorporating the amino acid tyrosine into the peptide or peptide mimetic and then iodinating the peptide (see, e.g., Weaner et al. (1994) Synthesis and Applications of lsotopically Label%d Compounds, pp. 137-140). If tyrosine is not present in the peptide or peptide mimetic, incorporation of tyrosine to the N
or C terminus of the peptide or peptide mimetic can be achieved by well known chemistry. Likewise, 3aP can be incorporated onto the peptide or peptide mimetic as a phosphate moiety through, for example, a hydroxyl group on the peptide or peptide mimetic using conventional chemistry.
Labeling of peptidomimetics usually involves covalent attachment of one or more labels, directly or through a spacer (e.g., an amide group), to non-interfering positions) on the peptidomimetic that are predicted by quantitative structure-activity data and/or molecular modeling. Such non-interfering positions generally are positions that do not form direct contacts with the macromolecules) to which the peptidomimetic binds to produce the therapeutic effect. Derivatization (e.g., labeling) of peptidomimetics should not substantially interfere with the desired biological or pharmacological activity of the peptidomimetic.
Peptides, polypeptides and peptide mimetics that can bind to a PKA
holoenzyme or the RI and/or RII subunit of PKA holoenzymes and/or modulate the activity thereof, can be used for treatment of neurodegenerative diseases, such as Alzheimer's Disease, cardiovascular disorders, cardiac disorders, particularly disorders associated with altered left ventricular function, cardiomyopathies, proliferative disorders, bipolar disorder and other neurological disorders, lipid-metabolism disorders, such as obesity, neoplastic disease, diabetes, certain peripheral retinopathies, such as retinitis pigmentosa, and autoimmune disorders, such as Lupus erythematosus. The peptides, polypeptides and peptide mimetics can be delivered, in vivo or ex vivo, to the cells of a subject in need of treatment. Further, peptides which have PKA
holoenzyme activity can be delivered, in vivo or ex vivo, to cells which carry mutant or missing alleles encoding the PKA holoenzyme gene. Any of the techniques described herein or known to the skilled artisan can be used for preparation and in vivo or ex vivo delivery of such peptides, polypeptides and peptide mimetics that are substantially free of other human proteins. For example, the peptides, polypeptides and peptide mimetics can be readily prepared by expression in a microorganism or synthesis in vitro.
In particular embodiments, the peptides, polypeptides, and peptide mimetics provided herein are able to permeate cell membranes and thus affect binding of PKA to a D-AKAP2. For example, a peptide or mimetic may be modified to include a fatty-acid moiety by conventional methods, either attached to either the amino terminus or the carboxy terminus of the peptide. Any fatty acid used in the art to achieve membrane-permeability of peptides may be employed, e.g., an N-stearylated peptide (Liotta et al. (1994) J. Biol. Chem., 269:22996-23001 ) or N-myristoylated peptide (O'Brian et al. (1990) Biochem.
Pharmacol., 39:49-57; Eicholtz et al. (1993) J. Biol. Chem., 268:1982-1986);
and the like. Fatty acid-peptide conjugates have been used to inhibit protein kinase C (PKC) and tyrosine kinase activities in intact cells (Eichholtz et al.
(1993) J. Biol. Chem., 268:1982-1986; Liotta et al. (1994) J. Biol. Chem., 269:22996-23001 ).
These peptides, polypeptides, and peptide mimetics may be introduced into cells by any conventional means. For example, a peptide may be incorporated into liposomes. Alternatively, the peptide can be formulated in a composition that includes an amphiphilic lipid, e.g., a head-to-tail amphiphile such as LipofectinT"" or a cationic facial amphiphile (CFA) (a conjugate of polyamines and bile-acid-based amphiphiles).
The peptides or peptide mimetics can be introduced into cells, in vivo or ex vivo, by microinjection or by use of liposomes, for example. Alternatively, the peptides, polypeptides or peptide mimetics can be taken up by cells, in vivo or ex vivo, actively or by diffusion. In addition, extracellular application of the peptide, polypeptide or peptide mimetic can be sufficient to effect treatment of neurodegeneratives diseases, such as Alzheimer's Disease, cardiovascular disorders, cardiac disorders, particularly disorders associated with altered left ventricular function, cardiomyopathies, proliferative disorders, bipolar disorder and other neurological disorders, lipid-metabolism disorders, such as obesity, neoplastic disease, diabetes, certain peripheral retinopathies, such as retinitis pigmentosa, and autoimmune disorders, such as Lupus erythematosus. Other molecules, such as drugs or organic compounds, that: 1 ) bind to a PKA
holoenzyme or RI and/or RII subunit thereof; or 2) have a similar function or activity to D-AKAP2 or a D-AKAP2 peptide or mimetic capable of binding a PKA
holoenzyme or RI and/or RII subunit thereof, can be used in methods for treatment.
E. Rational drug design The goal of rational drug design is to produce structural analogs of biologically active polypeptides or peptides of interest or of small molecules or peptide mimetics with which they interact (e.g., agonists and antagonists) in order to fashion drugs which are, e.g., more active or stable forms thereof;
or which, for example, enhance or interfere with the function of a polypeptide in vivo (e.g., a PKA holoenzymel. In one approach, one first determines the three-dimensional structure of a protein of interest (e.g., a PKA holoenzyme or polypeptide having a RI and/or RII subunit) or, for example, of a PKA
holoenzyme-D-AKAP2 complex, by ?C-ray crystallography, by computer modeling or most typically, by a combination of approaches. Also, useful information regarding the structure of a polypeptide can be gained by modeling based on the structure of homologous proteins. In addition, peptides can be analyzed by an alanine scan. In this technique, an amino acid residue is replaced by Ala, and its effect on the peptide's activity is determined. Each of the amino acid residues of the peptide is analyzed in this manner to determine the important regions of the peptide.
Also, a polypeptide or peptide that binds to a PKA holoenzyme or, generally, the RI and/or RII subunit of a PKA holoenzyme, can be selected by a functional assay, and then the crystal structure of this polypeptide or peptide can be determined. This approach can yield a pharmacophore upon which subsequent drug design can be based. Further, it is possible to bypass the crystallography altogether by generating anti-idiotypic polypeptides or peptides, (anti-ids) to a functional, pharmacologically active polypeptide or peptide that binds to a PKA holoenzyme or RI and/or RII subunit of a PKA holoenzyme. As a mirror image of a mirror image, the binding site of the anti-ids is expected to be an analog of the original target molecule, e.g., a PKA holoenzyme or polypeptide having a PKA holoenzyme. The anti-id can then be used to identify and isolate peptides from banks of chemically or biologically produced banks of peptides.
Selected peptides would then act as the pharmacophore.
Thus, one can design drugs which have, for example, improved activity or stability or which act as modulators (e.g., inhibitors, agonists or antagonists) of PKA holoenzyme activity, and are useful in the methods, particularly the methods for diagnosis, treatment, prevention, and screening of neurodegeneratives diseases, such as Alzheimer's Disease, cardiovascular disorders, cardiac disorders, particularly disorders associated with altered left ventricular function, cardiomyopathies, proliferative disorders, bipolar disorder and other neurological disorders, lipid-metabolism disorders, such as obesity, neoplastic disease, diabetes, certain peripheral retinopathies, such as retinitis pigmentosa, and autoimmune disorders, such as Lupus erythematosus. By virtue of the availability of nucleic acid that encodes PKA holoenzymes, sufficient amounts of the PKA holoenzyme can be made available to perform such analytical studies as X-ray crystallography. In addition, the knowledge of the amino acid sequence of a PKA holoenzyme or the RI and/or RII subunit thereof, e.g., the RI and/or RII subunit, can provide guidance on computer modeling techniques in place of, or in addition to, X-ray crystallography.
1. Methods of identifying additional peptides and peptide mimetics that bind to PKA holoenzymes In addition to the D-AKAP2 derived peptides provided herein, other peptides having a differential binding affinity to the RI and/or RII subunits of PKA
holoenzyme can be readily identified, for example, by random peptide diversity generating systems coupled with an affinity enrichment process. Specifically, random peptide diversity generating systems include the "peptides on plasmids"
system (see, e.g., U.S. Pat. Nos. 5,270,170 and 5,338,665); the "peptides on phage" system (see, e.g., U.S. Pat. No. 6,121,238 and Cwirla et al. (1990) Proc. Nat/. Acad. Sci. USA, 87:6378-6382); the "polysome system;" the "encoded synthetic library (ESL)" system; and the "very large scale immobilized polymer synthesis" system (see, e.g., U.S. Pat. No. 6,121,238; and Dower et al.
( 1991 ) An. Rep. Med. Chem., 26:271-280.
For example, using the procedures described above, random peptides can generally be designed to have a defined number of amino acid residues in length (e.g., 12). To generate the collection of oligonucleotides encoding the random peptides, the codon motif (NNK)x, where N is nucleotide A, C, G, or T
(equimolar; depending on the methodology employed, other nucleotides can be employed), K is G or T (equimolar), and x is an integer corresponding to the number of amino acids in the peptide (e.g., 12) can be used to specify any one of the 32 possible codons resulting from the NNK motif: 1 for each of 12 amino acids, 2 for each of 5 amino acids, 3 for each of 3 amino acids, and only one of the three stop codons. Thus, the NNK motif encodes all of the amino acids, encodes only one stop codon, and reduces codon bias.
The random peptides can be presented, for example, either on the surface of a phage particle, as part of a fusion protein containing either the plll or the pVlll coat protein of a phage fd derivative (peptides on phage) or as a fusion protein with the Lacl peptide fusion protein bound to a plasmid (peptides on plasmids). The phage or plasmids, including the DNA encoding the peptides, can be identified and isolated by an affinity enrichment process using immobilized PKA holoenzyme having a RI and/or RII subunit. The affinity enrichment process, sometimes called "panning," typically involves multiple rounds of incubating the phage, plasmids, or polysomes with the immobilized PKA
holoenzyme or RI and/or RII subunits thereof, collecting the phage, plasmids, or polysomes that bind to the PKA holoenzyme (along with the accompanying DNA
or mRNA), and producing more of the phage or plasmids (along with the accompanying Lacl-peptide fusion protein) collected.
2. Characteristics of peptides and peptide mimetics Among the peptides, polypeptides and peptide mimetics for therapeutic application are those of having molecular weights from about 250 to about 8,000 daltons. If such peptides are oligomerized, dimerized and/or derivatized with a hydrophilic polymer (e.g., to increase the affinity and/or activity of the compounds), the molecular weights of such peptides can be substantially greater and can range anywhere from about 500 to about 120,000 daltons, generally from about 8,000 to about 80,000 daltons. Such peptides can contain 9 or more amino acids that are naturally occurring or synthetic (non-naturally occurring) amino acids. One skilled in the art can determine the affinity and molecular weight of the peptides and peptide mimetics suitable for therapeutic and/or diagnostic purposes (e.g., see Dower et al., U.S. Pat. No. 6,121,238).
The peptides can be covalently attached to one or more of a variety of hydrophilic polymers. Suitable hydrophilic polymers include, but are not limited to, polyalkylethers as exemplified by polyethylene glycol and polypropylene glycol, polylactic acid, polyglycolic acid, polyoxyalkenes, polyvinylalcohol, polyvinylpyrrolidone, cellulose and cellulose derivatives, dextran and dextran derivatives. When the peptide compounds are derivatized with such polymers, their solubility and circulation half-lives can be increased with little, if any, diminishment in their binding activity. The peptide compounds can be dimerized and each of the dimeric subunits can be covalently attached to a hydrophilic polymer. The peptide compounds can be PEGylated, i.e,, covalently attached to polyethylene glycol (PEG).
F. Methods of preparing peptides and peptide mimetics D-AICAP2 based peptides provided herein that bind to PICA holoenzymes can be prepared by classical methods known in the art, for example, by using standard solid phase techniques. The standard methods include exclusive solid phase synthesis, partial solid phase synthesis methods, fragment condensation, classical solution synthesis, and even by recombinant DNA technology (see, e.g., Merrifield (1963) J. Am. Chem. Soc,, 85:2149, incorporated herein by reference.) Using the "encoded synthetic library" or "very large scale immobilized polymer synthesis" systems (see, e.g., U.S. Pat. Nos. 5,925,525, and 5,902,723), the minimum size of a peptide with the activity of interest can be determined. In addition all peptides that form the group of peptides that differ from the desired motif (or the minimum size of that motif) in one, two, or more residues can be prepared. This collection of peptides then can be screened for the ability to bind to the target molecule, e.g., PICA holoenzyme or, generally, the RI and/or RII subunit of a PKA holoenzyme. This immobilized polymer synthesis system or other peptide synthesis methods can also be used to synthesize truncation analogs and deletion analogs and combinations of truncation and deletion analogs of the peptide compounds.
These procedures can also be used to synthesize peptides in which amino acids other than the 20 naturally occurring, genetically encoded amino acids are substituted at one, two, or more positions of the peptide. For instance, naphthylalanine can be substituted for tryptophan, facilitating synthesis.
Other synthetic amino acids that can be substituted into the peptides include L-hydroxypropyl, L-3, 4-dihydroxy-phenylalanyl, d amino acids such as L-d-hydroxylysyl and D-d-methylalanyl, L-a-methylalanyl, /3 amino acids, and isoquinolyl. D amino acids and non-naturally occurring synthetic amino acids can also be incorporated into the peptides (see, e.g., Roberts et al. (1983) Unusual Amino/Acids in Peptide Synthesis, 5(6):341-449).
The peptides can also be modified by phosphorylation (see, e.g., W.
Bannwarth et al. (1996) Biorganie and Medicinal Chemistry Letters, 6 17 :2141-2146), and other methods for making peptide derivatives (see, e.g., Hruby et al. (1990) Biochem. J., 268 2 :249-262). Thus, peptide compounds also serve as a basis to prepare peptide mimetics with similar or improved biological activity.
Those of skill in the art recognize that a variety of techniques are available for constructing peptide mimetics with the same or similar desired biological activity as the corresponding peptide compound but with more favorable activity than the peptide with respect to solubility, stability, and susceptibility to hydrolysis and proteolysis (see, e.g., Morgan et al, (1989) An.
Rep. Med. Chem., 24:243-252). Methods for preparing peptide mimetics modified at the N-terminal amino group, the C-terminal carboxyl group, and/or changing one or more of the amido linkages in the peptide to a non-amido linkage are known to those of skill in the art.
Amino terminus modifications include, but are not limited to, alkylating, acetylating and adding a carbobenzoyl group, forming a succinimide group (see, e.g., Murray et al. (1995) Burger's Medicinal Chemistry and Drua Discovery, 5th ed., Vol. 1, Manfred E. Wolf, ed., John Wiley and Sons, Inc.). C-terminal modifications include mimetics wherein the C-terminal carboxyl group is replaced by an ester, an amide or modifications to form a cyclic peptide.
In addition to N-terminal and C-terminal modifications, the peptide compounds, including peptide mimetics, can advantageously be modified with or covalently coupled to one or more of a variety of hydrophilic polymers. It has been found that when peptide compounds are derivatized with a hydrophilic polymer, their solubility and circulation half-lives can be increased and their immunogenicity is masked, with little, if any, diminishment in their binding activity. Suitable nonproteinaceous polymers include, but are not limited to, polyalkylethers as exemplified by polyethylene glycol and polypropylene glycol, polylactic acid, polyglycolic acid, polyoxyalkenes, polyvinylalcohol, polyvinylpyrrolidone, cellulose and cellulose derivatives, dextran and dextran derivatives. Generally, such hydrophilic polymers have an average molecular weight ranging from about 500 to about 100,000 daltons, including from about 2,000 to about 40,000 daltons and, from about 5,000 to about 20,000 daltons.
The hydrophilic polymers also can have an average molecular weights of about 5,000 daltons, 10,000 daltons and 20,000 daltons.
Methods for derivatizing peptide compounds or for coupling peptides to such polymers have been described (see, e.g., Zallipsky (1995) Bioconjugate Chem.; 6:150-165; Monfardini et al. (1995) Bioconjugate Chem., 6:62-69; U.S.
Pat. No. 4,640,835; U.S. Pat. No. 4,496,689; U.S. Pat. No. 4,301,144; U.S.
Pat. No. 4,670,417; U.S. Pat. No. 4,791,192; U.S. Pat. No. 4,179,337 and W095/34326, all of which are incorporated by reference in their entirety herein).
Other methods for making peptide derivatives are described, for example, in Hruby et al. (1990) Biochem J., 26- 8(2):249-262, which is incorporated herein by reference. Thus, the peptide compounds also serve as structural models for non-peptidic compounds with similar biological activity. Those of skill in the art recognize that a variety of techniques are available for constructing compounds with the same or similar desired biological activity as a particular peptide compound but with more favorable activity with respect to solubility, stability, and susceptibility to hydrolysis and proteolysis (see, e.g., Morgan et al.
(1989) An. Rep. Med. Chem., 24:243-252, incorporated herein by reference). These techniques include replacing the peptide backbone with a backbone composed of phosphonates, amidates, carbamates, sulfonamides, secondary amines, and N-methylamino acids.
Peptide compounds can exist in a cyclized form with an intramolecular disulfide bond between the thiol groups of the cysteines. Alternatively, an intermolecular disulfide bond between the thiol groups of the cysteines can be produced to yield a dimeric (or higher oligomeric) compound. One or more of the cysteine residues can also be substituted with a homocysteine.
G. Transgenic Animals Methods for making transgenic animals using a variety of transgenes have been described in Wagner et al. ( 1981 ) Proc. Nat. Acad. Sci. USA, 78:5016-5020; Stewart et al. (1982) Science, 217:1046-1048; Constantini et a/. (1981 ) Nature, 294:92-94; Lacy et al. (1983) Cell, 34:343-358; McKnight et a/. (1983) Cell, 34:335-341; Brinstar et al. (1983) Nature, 306:332-336;
Palmiter et al. (1982) Nature, 300:611-615; Palmiter et al. (1982) Cell, 29:701-710, and Palmiter et al. (1983) Science, 222:809-814. Such methods are described in U.S. Pat. Nos. 6,175,057; 6,180,849; and 6,133,502.
The term "transgene" is used herein to describe genetic material that has been or is about to be artificially inserted into the genome of a mammalian cell, particularly a mammalian cell of a living animal. The transgene is used to transform a cell, meaning that a permanent or transient genetic change, preferably a permanent genetic change, is induced in a cell following incorporation of exogenous DNA. A permanent genetic change is generally achieved by introduction of the DNA into the genome of the cell. Vectors for stable integration include, but are not limited to, plasmids, retroviruses and other animal viruses and YACS. Of interest are transgenic mammals, including, but are not limited to, cows, pigs, goats, horses and others, and particularly rodents, including rats and mice. Preferably, the transgenic-animals are mice.
Transgenic animals contain an exogenous nucleic acid sequence present as an extrachromosomal element or stably integrated in all or a portion of its cells, especially germ cells. Unless otherwise indicated, it will be assumed that a transgenic animal comprises stable changes to the germline sequence. During the initial construction of the animal, "chimeras" or "chimeric animals" are generated, in which only a subset of cells have the altered genome. Chimeras are primarily used for breeding purposes in order to generate the desired transgenic animal. Animals having a heterozygous alteration are generated by breeding of chimeras. Male and female heterozygotes are typically bred to generate homozygous animals.
The exogenous gene is usually either from a different species than the animal host, or is otherwise altered in its coding or non-coding sequence. The introduced gene may be a wild-type gene, naturally occurring polymorphism or a genetically manipulated sequence, for example having deletions, substitutions or insertions in the coding or non-coding regions. When the introduced gene is a coding sequence, it is usually operably linked to a promoter, which may be constitutive or inducible, and other regulatory sequences required for expression in the host animal.
Transgenic animals can comprise other genetic alterations in addition to the presence of alleles of AKAP genes. For example, the genome can be altered to affect the function of the endogenous genes, contain marker genes, or contain other genetic alterations (e.g., alleles of genes associated with cardiovascular disease).
A "knock-out" of a gene means an alteration in the sequence of the gene that results in a decrease of function of the target gene, preferably such that target gene expression is undetectable or insignificant. A knock-out of an endogenous AKAP gene means that function of the gene has been substantially decreased so that expression is not detectable or only present at insignificant levels. "Knock-out" transgenics can be transgenic animals having a heterozygous knock-out of an AKAP gene or a homozygous knock-out. "Knock-outs" also include conditional knock-outs. As used herein, "conditional" in reference to "knock-outs" and "knock-ins" means alteration of the target gene can occur upon, for example, exposure of the animal to a substance that promotes target gene alteration, introduction of an enzyme that promotes recombination at the target gene site (e.g., Cre in the Cre-lox system), or other method for directing the target gene alteration postnatally.
A "knock-in" transgenic animal refers to an animal that has had a modified gene introduced into its genome, wherein the modified gene can be of exogenous or endogenous origin (see, e.g., Roemer et al. (1991 ) Nevv Biol., _3:331 ). Accordingly, a "knock-in" of a target gene means an alteration in a host cell genome that results in either expression of an altered target gene; or altered expression (e.g., increased (including ectopic)) of the target gene, e.g., by introduction of an additional copy of the target gene, or by operatively inserting a regulatory sequence that provides for enhanced expression of an endogenous copy of the target gene. "Knock-in" transgenics of interest can be transgenic animals having a knock-in of an AKAP gene. Such transgenics can be heterozygous or homozygous for the knock-in gene. "Knock-ins" also encompass conditional knock-ins. As used herein, "knock-in" transgenic animals also encompasses animals in which an animal gene is replaced by the human equivalent within the genome of that animal. These transgenic knock-in animals are useful for drug discovery, for target validation, where the compound is specific for the human target. Transgenic knock-ins can by produced using homologous recombination, using transposons (e.g., Westphal et al. (1997) Curr.
Biol., 7:530, and the like), using mutant recombination sites (e.g., Araki et al.
(1997) NAR, 25:868, and the like), using PCR (e.g., Zhang et al.
Biotechnigues, 25:784, and the like), and the like.
For example, the binding data generated herein related to certain amino acid positions within the 27-mer AKB binding domain is used to knock-in specific amino acids at specific positions in the genome of cells and organisms (e.g.
animals, cells) to change the binding ratio of both PKA-Rla and PKA-Rlla subunits to its target protein D-AKAP2 in vivo. Such a changed binding ratio is contemplated herein to produce a changed phenotype useful to elucidate the biological function of D-AKAP2 and PKA subunits and their contribution to the manifestation of diseases set forth herein. Likewise, the binding data generated herein related to certain amino acid positions within the 27-mer AKB binding domain is used to knock-in specific amino acids at specific positions in the genome of cells and organisms (e,g., animals, cells) to disrupt the binding of one particular PKA-R subunit isoform to D-AKAP2 in vivo. Such a binding disruption is contemplated herein to cause a specific loss of function of D-AKAP2 resulting in a changed phenotype useful to elucidate the biological function of D-AKAP2 and PKA subunits and their contribution to the manifestation of diseases set forth herein.
The transgenic knock-in cellular systems or animals provided herein are useful to screen chemical compound libraries for the purpose of drug discovery.
In addition, these transgenic knock-in cellular systems or animals are useful in assays along the entire drug development process.
A construct is suitable for use in the generation of transgenic animals if it allows the desired level of expression of an AKAP encoding sequence. Methods of isolating and cloning a desired sequence, as well as suitable constructs for expression of a selected sequence in a host animal, are well known in the art and are described below.
For the introduction of a gene into the subject animal, it is generally advantageous to use the gene as a gene construct wherein the gene is ligated downstream of a promoter capable of and operably linked to expressing the gene in the subject animal cells. Specifically, a transgenic non-human mammal showing high expression of the desired gene can be created by microinjecting a vector ligated with said gene into a fertilized egg of the subject non-human mammal (e,g., rat fertilized egg) downstream of various promoters capable of expressing the protein and/or the corresponding protein derived from various mammals (rabbits, dogs, cats, guinea pigs, hamsters, rats, mice etc., preferably rats ete.).
Useful vectors include Escherichia coli-derived plasmids, Bacillus subtilis-derived plasmids, yeast-derived plasmids, bacteriophages such as lambda, phage, retroviruses such as Moloney leukemia virus, and animal viruses such as vaccinia virus or baculovirus.
Useful promoters for such gene expression regulation include, for example, promoters for genes derived from viruses (cytomegalovirus, Moloney leukemia virus, JC virus, breast cancer virus, etc.), and promoters for genes derived from various mammals (humans, rabbits, dogs, cats, guinea pigs, hamsters, rats, mice, etc. ), and birds (chickens, etc. ) (e.g., genes for albumin, insulin II, erythropoietin, endothelin, osteocalcin, muscular creatine kinase, platelet-derived growth factor beta, keratins K1, K10 and K14, collagen types I
and II, atrial natriuretic factor, dopamine beta-hydroxylase, endothelial receptor tyrosine kinase (generally abbreviated Tie2), sodium-potassium adenosine triphosphorylase (generally abbreviated Na,K-ATPase), neurofilament light chain, metallothioneins I and IIA, metalloproteinase I tissue inhibitor, MHC class I
antigen (generally abbreviated H-2L), smooth muscle alpha actin, polypeptide chain elongation factor 1 alpha (EF-1 alpha), beta actin, alpha and beta myosin heavy chains, myosin light chains 1 and 2, myelin base protein, serum amyloid component, myoglobin, renin, etc.).
It is preferable that the above-mentioned vectors have a sequence for terminating the transcription of the desired messenger RNA in the transgenic animal (generally referred to as terminator); for example, gene expression can be manipulated using a sequence with such function contained in various genes derived from viruses, mammals and birds. Preferably, the simian virus SV40 terminator, etc., are commonly used. Additionally, for the purpose of increasing the expression of the desired gene, the splicing signal and enhancer region of each gene, a portion of the intron of a eukaryotic organism gene may be ligated 5' upstream of the promoter region, or between the promoter region and the translational region, or 3' downstream of the translational region as desired.
A translational region for a protein of interest can be obtained using the entire or portion of genomic DNA of blood, kidney or fibroblast origin from various mammals (humans, rabbits, dogs, cats, guinea pigs, hamsters, rats, mice, etc.), or of various commercially available genomic DNA libraries, as a starting material, or using complementary DNA prepared by a known method from RNA of blood, kidney or fibroblast origin as a starting material. Also, an exogenous gene can be obtained using complementary DNA prepared by a known method from RNA of human fibroblast origin as a starting material. All these translational regions can be used in transgenic animals.

To obtain the translational region, it is possible to prepare DNA
incorporating an exogenous gene encoding the protein of interest in which the gene is ligated downstream of the above-mentioned promoter (preferably upstream of the translation termination site) as a gene construct capable of being expressed in the transgenic animal.
DNA constructs for random integration need not include regions of homology to mediate recombination. Where homologous recombination is desired, the DNA constructs will comprise at least a portion of the target gene with the desired genetic modification, and will include regions of homology to the target locus. Conveniently, markers for positive and negative selection are included. Methods for generating cells having targeted gene modifications through homologous recombination are known in the art. For various techniques for transfecting mammalian cells, see Keown et al. (1990) Meth. Enzymol., 185:527-537.
The transgenic animal can be created by introducing an AKAP gene construct into, for example, an unfertilized egg, a fertilized egg, a spermatozoon or a germinal cell containing a primordial germinal cell thereof, preferably in the embryogenic stage in the development of a non-human mammal (more preferably in the single-cell or fertilized cell stage and generally before the 8-cell phase), by standard means, such as the calcium phosphate method, the electric pulse method, the lipofection method, the agglutination method, the microinjection method, the particle gun method, the DEAE-dextran method and other such method. Also, it is possible to introduce a desired AKAP gene into a somatic cell, a living organ, a tissue cell or other cell, by gene transformation methods, and use it for cell culture, tissue culture and any other method of propagation.
Furthermore, these cells may be fused with the above-described germinal cell by a commonly known cell fusion method to create a transgenic animal.
For embryonic stem (ES) cells, an ES cell line may be employed, or embryonic cells may be obtained freshly from a host, e.g., mouse, rat, guinea pig, etc. Such cells are grown on an appropriate fibroblast-feeder layer or grown in the presence of appropriate growth factors, such as leukemia inhibiting factor (LIF). When ES cells have been transformed, they may be used to produce transgenic animals. After transformation, the cells are plated onto a feeder layer in an appropriate medium. Cells containing the construct may be detected by employing a selective medium. After sufficient time for colonies to grow, they are picked and analyzed for the occurrence of homologous recombination or integration of the construct. Those colonies that are positive may then be used for embryo manipulation and blastocyst injection. Blastocysts are obtained from 4 to 6 week old superovulated females. The ES cells are trypsinized, and the modified cells are injected into the blastocoel of the blastocyst. After injection, the blastocysts are returned to each uterine horn of pseudopregnant females.
Females are then allowed to go to term and the resulting litters screened for mutant cells having the construct. By providing for a different phenotype of the blastocyst and the ES cells, chimeric progeny can be readily detected. The chimeric animals are screened for the presence of the modified gene and males and females having the modification are mated to produce homozygous progeny.
If the gene alterations cause lethality at some point in development, tissues or organs can be maintained as allogeneic or congenic grafts or transplants, or in vitro culture.
Animals containing more than one transgene, such as allelic variants of AKAP genes and/or other genes associated with morbidity and/or mortality can be made by sequentially introducing individual alleles into an animal in order to produce the desired phenotype (manifestation of morbidity and/or predisposition to early mortality). In addition, animals containing one or more amino acid substitutions with that same transgene, such as D-AKAP2 can be made by sequentially introducing individual amino acid substitutions, or introducing one or more substitutions in a single construct, into an animal in order to produce the desired genotype and/or phenotype (manifestation of morbidity and/or predisposition to early mortality). For example, transgenic animals having one or more amino acid substitutions in the D-AKAP2 gene, include transgenic animals having a combination of 2, 3, 4, 5, 6, 7, 8, 9, 10 or more of the amino acid residue substitutions set forth herein, such as, but not limited to, those described above and in the Examples, or in the claims.

Accordingly, provided herein are transgenic non-human knock-in animals comprising a gene encoding the animal homolog of human D-AKAP2, wherein said gene has been modified to encode any combination of one or more amino acid substitutions in a 27-mer PKA binding region of the animal protein homolog of human D-AKAP2 corresponding to amino acids 623-649 of human D-AKAP2 set forth in SEQ. ID NOs:63-65; or SEQ ID NOs:1 or 2. In one embodiment, the amino acid substitution in the animal homolog of human D-AKAP2 is selected from the group consisting of substitutions that correspond to the substitution in SEQ ID NOs:63-65 of one, two or more: of Q at residue 631 with F, I, L, V, H, M, R, T, W or Y; of L at residue 634 with F, W or Y; of V at residue 643 with I, L or W; and of M at residue 647 with F, I, L, T, V, W or Y. In this embodiment, the modified animal protein homolog of human D-AKAP2 can exhibit a preferred or exclusive binding to PKA-Rla subunits relative to PKA-Rlla subunits, or enhanced binding to both Rla and Rlla subunits, compared to the native unmodified animal protein homolog of human D-AKAP2.
In another embodiment, the amino acid substitution in the animal homolog of human D-AKAP2 is selected from the group consisting of substitutions that correspond to the substitution in SEQ ID NOs:63-65 of one, two or more: of L
at residue 634 with A, C, or K; of A at residue 635 with F, H, I, K, L, M, N or S; of W at residue 636 with C; of K at residue 637 with C; of K at residue 640 with C; of M at residue 641 with C; of S at residue 644 with C; and of D at residue 645 with C. In this embodiment, the modified animal protein homolog of human D-AKAP2 can exhibit a preferred or exclusive binding to PKA-Rlla subunits relative to PKA-Rla subunits, compared to the native unmodified animal protein homolog of human D-AKAP2.
In another embodiment, the animal is a mouse, and wherein the amino acid substitution in the mouse D-AKAP2 is selected from the group consisting of substitutions that correspond to the substitution in SEQ ID N0:55 or 56 of one, two or more: of Q at residue 341 with F, I, L, V, H, M, R, T, W or Y; of L at residue 344 with F, W or Y; of V at residue 353 with I, L or W; and of M at residue 357 with F, I, L, T, V, W or Y. In this embodiment, the modified animal protein homolog of human D-AKAP2 is mouse D-AKAP2 that exhibits a preferred or exclusive binding to PKA-Rla subunits relative to PKA-Rlla subunits, or enhanced binding to both Rla and Rlla subunits, compared to the native unmodified mouse D-AKAP2. In a particular embodiment, the amino acid substitution in the mouse D-AKAP2 corresponds to a single amino acid substitution in SEQ ID N0:55 or 56 of Q at residue 341 with I, and wherein the modified mouse D-AKAP2 exhibits a enhanced binding to PKA-Rla subunits, and normal binding to PKA-Rlla subunits compared to the native unmodified mouse D-AKAP2. In another embodiment, the amino acid substitution in the mouse D-AKAP2 corresponds to a single amino acid substitution in SEQ ID N0:55 or 56 of V at residue 353 with W, and wherein the modified mouse D-AKAP2 exhibits a normal binding to PKA-Rla subunits, and disrupted or decreased binding to PKA-Rlla subunits compared to the native unmodified mouse D-AKAP2. In another embodiment, the amino acid substitution in the mouse D-AKAP2 corresponds to a triple amino acid substitution in SEQ. ID N0:55 or 56 of Q at residue 341 with F, of V at residue 353 with W, and of M at residue 357 with F, and wherein the modified mouse D-AKAP2 exhibits increased binding to Rla and decreased binding affinity for Rlla. In a particular embodiment, the modified mouse D-AKAP2 having the triple amino acid substitution exhibits approximately 10-fold increased binding to Rla and approximately 220-fold decreased binding affinity for Rlla.
In another embodiment, the animal is a mouse, and wherein the modified animal protein homolog of human D-AKAP2 is mouse D-AKAP2 that exhibits a preferred or exclusive binding to PKA-Rlla subunits relative to PKA-Rla subunits, compared to the native unmodified mouse D-AKAP2; and wherein the amino acid substitution in the mouse D-AKAP2 is selected from the group consisting of substitutions that correspond to the substitution in SEQ ID N0:55 or 56 of one, two or more: of L at residue 344 with A, C, or K; of A at residue 345 with F, H, I, K, L, M, N or S; of W at residue 346 with C; of K at residue 347 with C; of K
at residue 350 with C; of M at residue 351 with C; of S at residue 354 with C;
and of D at residue 355 with C. In a particular embodiment, the amino acid substitution in the mouse D-AKAP2 corresponds to a single amino acid substitution in SEQ ID N0;55 or 56 of A at residue 345 with L, and wherein the modified mouse D-AKAP2 exhibits normal binding to PKA-Rlla subunits, and disrupted binding to PKA-Rla subunits compared to the native unmodified mouse D-AKAP2.
H. Protein and Polypeptide Detection 1. Expression of Protein in a Cell Line Using the nucleic acids described herein, variant D-AKAP2 proteins (referred to herein as D-AKAP2 muteins) may be expressed in a recombinantly engineered cell such as bacteria, yeast, insect, mammalian, or plant cells.
Those of skill in the art are knowledgeable in the numerous expression systems available for expression of a nucleic acid encoding proteins such as polymorphic human D-AKAP2 proteins. Accordingly, provided herein are cells, comprising heterologous nucleic acid that encodes a mammalian D-AKAP2 variant protein or portion that exhibits a preferred or exclusive binding to PKA-Rla subunits relative to PKA-Rlla subunits; or enhanced binding to both Rla and Rllcr subunits, compared to the unmodified full length D-AKAP2 protein or SEQ ID NOs:1 or 2.
In one embodiment, the D-AKAP2 variant protein or portion thereof comprises at least one single amino acid substitution in the mammalian D-AKAP2 selected from the group consisting of substitutions that correspond to the substitution in SEQ ID NOs:64 or 65 of one, two or more: of Q at residue 631 with F, I, L, V, H, M, R, T, W or Y; of L at residue 634 with F, W or Y; of V at residue 643 with I, L or W; and of M at residue C47 with F, I, L, T, V, W or Y.
Also provided herein are cells comprising heterologous nucleic acid that encodes a mammalian D-AKAP2 variant protein or portion that exhibits a preferred or exclusive binding to PKA-Rlla subunits relative to PKA-Rla subunits, compared to the unmodified full length D-AKAP2 protein or SEQ ID NOs:1 or 2.
In one embodiment, the D-AKAP2 variant protein or portion thereof comprises at least one single amino acid substitution in the mammalian D-AKAP2 selected from the group consisting of substitutions that correspond to the substitution in SEQ ID NOs:64 or 65 of one, two or more: of L at residue 634 with A, C, or K;
of A at residue 635 with F, H, I, K, L, M, N or S; of W at residue 636 with C;
of K at residue 637 with C; of K at residue 640 with C; of M at residue 641 with C; of S at residue 644 with C; and of D at residue 645 with C.

Also provided herein are cells, comprising heterologous nucleic acid that encodes a mammalian D-AKAP2 variant protein or portion comprising any one or more of the peptide sequences provided herein at the corresponding region in the mammalian D-AKAP2 variant protein.
2. Expression of variant D-AKAP2 Proteins Provided herein are D-AKAP2 muteins, or polypeptide fragments thereof, comprising a peptide region corresponding to an A-Kinsase binding (AKB) domain set forth as amino acids 623-649 of SEQ ID NOs:64 or 65. In one embodiment, the sequence of said AKB domain peptide region corresponds to any of the peptide sequences described herein and in the Examples as well as in the claims.
In one embodiment, the D-AKAP2 mutein can have 28 or more amino acid residues, wherein the amino acid sequence of the D-AKAP2 mutein additional to AKB domain peptide region, is 1 to 635 amino acids in length and corresponds to a contiguous region from amino acids 1-622 andlor 650-662 of SEO. ID
NOs:64 or 65. In another embodiment, the D-AKAP2 mutein can be 662 amino acids in length, and further comprises amino acids 1-622 and 650-662 of SEQ
ID NOs:64 or 65. Also provided are isolated nucleic acid molecules, vectors, and cells containing these vectors, comprising a sequence of nucleotides that encodes the D-AKAP2 mutein provided herein. Also provided herein are methods of producing a D-AKAP2 mutein by growing the cells comprising a vector under conditions whereby the D-AKAP2 mutein is expressed; and isolating the mutein. The cell can be any animal cell (e.g., mammalian or human), yeast cell, insect cell or bacterial cell.
The isolated nucleic acid encoding a full-length mammalian (e.g., human, mouse, and the like) D-AKAP2 protein muteins provided herein, or a portion thereof, such as a peptide fragment containing one or more of the biologically significant variant sites set forth herein in Examples 5-9, may be introduced into a vector for transfer into host cells. Fragments of the polymorphic mammalan, e.g., human, D-AKAP2 proteins can be produced by those skilled in the art, without undue experimentation, by eliminating portions of the coding sequence from the isolated nucleic acids encoding the full-length proteins.

The isolated nucleic acid encoding a full length D-AKAP2 protein, mutein or portion thereof can be modified to use a preferred codon bias to increase the expression level of the AKAP protein. The codon usage of the target organism or cell for expression can be determined by methods such as described in U.S.
Pat. Nos. 5,082,767 and 4,562,639 (incorporated herein by reference). The isolated nucleic acid can then be modified by mutagenesis, recombination, or produced by synthetic DNA synthesis or other techniques known in the art such that the modified nucleic acid encoding the D-AKAP2 protein, mutein or portion thereof has at least one codon optimized for expression in the target organism or host cell. Using such methods the expression of D-AKAP2 protein, mutein or portion thereof can be increased above the expression of the unmodified sequences. Conversely, if lower expression is desired, the codon usage of the nucleic acid encoding the D-AKAP2 protein, mutein or portion thereof can be modified to select for non-preferred or less preferred codons of the target organism or cell for expression such as by methods decribed in U.S. Pat. Nos.
5,786,464 and 6,1 14,148 (incorporated herein by reference).
Expression vectors are used to express the protein in the desired host cell. An expression vector includes vectors capable of expressing nucleic acids that are operatively linked with regulatory sequences, such as promoter regions, that are capable of effecting expression of such nucleic acids. Thus, an expression vector refers to a recombinant DNA or RNA construct, such as a plasmid, a phage, recombinant virus or other vector that, upon introduction into an appropriate host cell, results in expression of the cloned DNA. Appropriate expression vectors are well known to those of skill in the art and include those that are replicable in eukaryotic cells and/or prokaryotic cells and those that remain episomal or those which integrate into the host cell genome. Such plasmids for expression of polymorphic mammalian, e.g,, human, D-AKAP2-encoding nucleic acids in eukaryotic host cells, particularly mammalian cells, include cytomegalovirus (CMV) promoter-containing vectors, such as PCMVS, the pSV2dhfr expression vectors, which contain the SV40 early promoter, mouse dhfr gene, SV40 polyadenylation and splice sites and sequences necessary for maintaining the vector in bacteria, and MMTV promoter-based vectors.
Expression vectors can be constructed which up-regulate or down-regulate expression of D-AKAP2, a mutein or portion thereof in a host cell or transgenic animal. Methods for down-regulation include antisense expression, RNAi constructs, ribozyme expression and other methods well known in the art.
Such expression vectors may include the full length nucleotide sequence of D-AKAP2 sequence or mutein provided herein or a portion thereof. Vectors can be designed that are specific for down-regulating expression a specific allele of D-AKAP2, for example down-regulation of the Val(646) variant of D-AKAP2.
Vectors can also be designed to down-regulate expression of all or most of alleles of D-AKAP2. Such vectors can also be designed to down-regulate D-AKAP2 homologs. Similarly, expression vectors can be designed which up-regulate D-AKAP2 expression or which express a high amount of a particular D-AKAP2 mutein or portion thereof. For example, promoters can be used which are known to regulate high levels of expression, for example viral promoters and other promoters such as described herein and known in the art. Codon optimization, as described above, can also be used to increase expression of full length D-AKAP2, a mutein or portion thereof. Another method of up-regulation is ectopic expression, the expression of D-AKAP2, a mutein or portion thereof in a cell-type or tissue that does not normally express D-AKAP2. Such ectopic expression can be accomplished by using tissue-specific or regulatable promoters. One example of such a regulatable promoter is the Tet-on/Tet-off system (available from Clontech, BD Biosciences, Palo Alto CA) in which gene expression is regulated by the administration of tetracycline or related analogs.
The nucleic acids encoding polymorphic human D-AKAP2 proteins, and vectors and cells containing the nucleic acids as provided herein permit production of the polymorphic protein variants, as well as antibodies to the proteins. This provides a means to prepare synthetic or recombinant polymorphic human D-AKAP2 proteins and fragments thereof that are substantially free of contamination from other AKAPs and proteins in general, the presence of which can interfere with analysis of the polymorphic proteins.

In addition, the polymorphic proteins may be expressed in combination with selected other proteins that D-AKAP2 may associate with in cells. The ability to selectively express the polymorphic D-AKAP2 proteins alone or in combination with other selected proteins makes it possible to observe the functioning of the recombinant polymorphic proteins within the environment of a cell. The expression of isolated nucleic acids encoding an AKAP protein will typically be achieved by operably linking, for example, the DNA or cDNA to a promoter (which is either constitutive or regulatable), followed by incorporation into an expression vector. The vectors can be suitable for replication and integration in either prokaryotes or eukaryotes. Typical expression vectors contain transcription and translation terminators, initiation sequences, and promoters useful for regulation of the expression of the DNA encoding a protein. To obtain high level expression of a cloned gene, it is desirable to construct expression vectors which contain, a strong promoter to direct transcription, a ribosome binding site for translational initiation, and a transcription/translation terminator.
One of skill in the art would recognize that modifications can be made to an D-AKAP2 protein without diminishing its biological activity. Some modifications may be made to facilitate the cloning, expression, or incorporation of the targeting molecule into a fusion protein. Such modifications are well known to those of skill in the art and include, for example, a methionine added at the amino terminus to provide an initiation site, or additional amino acids (e.g., poly His) placed on either terminus to create conveniently located purification sequences. Restriction sites or termination codons can also be introduced.
There are expression vectors that specifically allow the expression of functional proteins. One such vector, Plasmid 577, described in U.S. Pat. No. 6,020,122 and incorporated herein by reference, has been constructed for the expression of secreted antigens in a permanent cell line. This plasmid contains the following DNA segments: (a) a fragment of pBR322 containing bacterial beta-lactamase and origin of DNA replication; (b) a cassette directing expression of a neomycin resistance gene under control of HSV-1 thymidine kinase promoter and poly-A
addition signals; (c) a cassette directing expression of a dihydrofolate reductase gene under the control of a SV-40 promoter and poly-A addition signals; (d) cassette directing expression of a rabbit immunoglobulin heavy chain signal sequence fused to a modified hepatitis C virus (HCV) E2 protein under the control of the Simian Virus 40 T-Ag promoter and transcription enhancer, the hepatitis B virus surface antigen (HBsAg) enhancer I followed by a fragment of Herpes Simplex Virus-1 (HSV-1 ) genome providing poly-A addition signals; and (e) a fragment of Simian Virus 40 genome late region of no function in this plasmid. All of the segments of the vector were assembled by standard methods known to those skilled in the art of molecular biology. Plasmids for the expression of secreted AKAP proteins can be constructed by replacing the hepatitis C virus E2 protein coding sequence in plasmid 577 with a AKAP
sequence of SEQ ID N0:63 or a fragment thereof. The resulting plasmid is transfected -into CHO/dhfr-cells (DXB-1 1 1 ) (Uriacio et al, (1980) PNAS, 77:4451-4466); these cells are available from the A.T.C.C., 12301 Parklawn Drive, Rockville, MD. 20852, under Accession No. CRL 9096), using the cationic liposome- -mediated procedure (P. L. Felgner et al. (1987) PNAS 84:7413-7417.
Proteins are secreted into the cell culture media.
Incorporation of cloned DNA into a suitable expression vector, transfection of cells with a plasmid vector or a combination of plasmid vectors, each encoding one or more distinct proteins or with linear DNA, and selection of transfected cells are well known in the art (see, e.g., Sambrook et al. (1989) Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press). Heterologous nucleic acid may be introduced into host cells by any method known to those of skill in the art, such as transfection with a vector encoding the heterologous nucleic acid by Calcium phosphate precipitation (see, e.g., Wigler et al. (1979) Proc. Nat/. Acad. Sci. USA, 76:1373-1376) or lipofectamine (GIBCO BRL #18324-012). Recombinant cells can then be cultured under conditions whereby the polymorphic human D-AKAP2 protein encoded by the nucleic acid is expressed. Suitable host cells include mammalian cells (e.g., HEK293, including but are not limited to, those described in U.S. Pat. No. 5,024,939 to Gorman (see, also, Stillman et al.
(1985) Mol. Cell. Biol., 5:2051-2060); also, HEK293 cells available from ATCC
under accession #CRL 1573), CHO, COS, BHKBI and Ltk- cells, mouse monocyte macrophage P388D1 and J774A-1 cells (available from ATCC, Rockville, MD) and others known to those of skill in this art), yeast cells, including, but are not limited to, Pichia pastoris, Saccharomyces cerevisiae, Candida tropicalis, Hansenula polymorpha, human cells, bacterial cells, including, but are not limited to, Escherichia coli, and insect cells. Xenopus oocytes may also be used for expression of in vitro RNA transcripts of the DNA.
Heterologous nucleic acid may be stably incorporated into cells or may be transiently expressed using methods known in the art. Stably transfected mammalian cells may be prepared by transfecting cells with an expression vector having a selectable marker gene (such as, for example, the gene for thymidine kinase, dihydrofolate reductase, neomycin resistance, and the like), and growing the transfected cells under conditions selective for cells expressing the marker gene. To prepare transient transfectants, mammalian cells are transfected with a reporter gene (such as the E. coli f3-galactosidase gene) to monitor transfection efficiency. Selectable marker genes are not included in the transient transfections because the transfectants are typically not grown under selective conditions, and are usually analyzed within a few days after transfection.
Heterologous nucleic acid may be maintained in the cell as an episomal element or may be integrated into chromosomal DNA of the cell. The resulting recombinant cells may then be cultured or subcultured (or passaged, in the case of mammalian cells) from such a culture or a subculture thereof. Methods for transfection, injection and culturing recombinant cells are known to the skilled artisan. Similarly, the polymorphic human D-AICAP2 proteins or fragments thereof may be purified using protein purification methods known to those of skill in the art. For example, antibodies or other ligands that specifically bind to the proteins may be used for affinity purification and immunoprecipitation of the proteins.
3. Protein Purification The D-AKAP2 proteins may be purified by standard techniques well known to those of skill in the art. Recombinantly produced proteins can be directly expressed or expressed as a fusion protein. The recombinant protein is purified by a combination of cell lysis (e.g., sonication, French press) and affinity chromatography. The proteins, recombinant or synthetic, may be purified to substantial purity by standard techniques well known in the art, including detergent solubilization, selective precipitation with such substances as ammonium sulfate, column chromatography, immunopurification methods, and others. (See, for example, R. Scopes, Protein Purification: Principles and Practice, Springer-Verlag: New York ( 1982); Deutscher, Guide to Protein Purification, Academic Press (1990)). For example, antibodies may be raised to the proteins as described herein. Purification from E. coli can be achieved following procedures described in U.S. Pat. No. 4,51 1,503. The protein may then be isolated from cells expressing the protein and further purified by standard protein chemistry techniques as described herein. Detection of the expressed protein is achieved by methods known in the art and include, for example, radioimmunoassays, Western blotting techniques or immunoprecipitation.
I. Immunodetection of variant D-AKAP2 protein species.
Generally, the variant regions of the variant AICAP protein species provided herein, when presented as an immunogen, should elicit production of a specifically reactive antibody. Immunoassays for determining binding are well known to those of skill in the art, as are methods of making and assaying for antibody binding specificity/affinity. Exemplary immunoassay formats include ELISA, competitive immunoassays, radioimmunoassays, Western blots, indirect immunofluorescent assays, in vivo expression or immunization protocols with purified protein preparations. In general, the detection of immunocomplex formation is well known in the art and may be achieved by methods generally based upon the detection of a label or marker, such as any of the radioactive, fluorescent, biological or enzymatic tags. Labels are well known to those skilled in the art (see U.S. Pat. Nos. 3,817,837; 3,850,752; 3,939,350; 3,996,345;
4,277,437; 4,275,149 and 4,366,241, each incorporated herein by reference).
Of course, one may find additional advantages through the use of a secondary binding ligand such as a second antibody or a biotin/avidin ligand binding arrangement, as is known in the art.
1. Production of Polyclonal Antisera Against specific variant AKAPs Antibodies can be raised to the variant AKAP protein species provided herein, including fragments thereof, both in their naturally occurring (full-length) forms and in recombinant forms. Additionally, antibodies are raised to these proteins in either their native configurations or in non-native configurations.
Anti-idiotypic antibodies can also be generated. A variety of analytic methods are available to generate a hydrophilicity profile of proteins. Such methods can be used to guide the artisan in the selection of peptides for use in the generation or selection of antibodies which are specifically reactive, under immunogenic conditions. See, e.g., J. Janin, (1979) Nature, 277:491-492; Wolfenden et al.
(1981 ) Biochemistry 20:849-855; ICyte and Doolite (1982) J. Mol. Biol., 157:105-132; Rose et al. (1985) Science, 229:834-838.
A number of immunogens can be used to produce antibodies specifically reactive with a particular variant AKAP protein species. An isolated recombinant, synthetic, or native polypeptide are the preferred immunogens (antigen) for the production of monoclonal or polyclonal antibodies.
Polypeptides are typically denatured, and optionally reduced, prior to formation of antibodies for screening expression libraries or other assays in which a putative AICAP
protein is expressed or denatured in a non-native secondary, tertiary, or quartenary structure.
The particular variant region of the variant AICAP protein is injected into an animal capable of producing antibodies. Either monoclonal or polyclonal antibodies can be generated for subsequent use in immunoassays to measure the presence and quantity of the protein. Methods of producing polyclonal antibodies are known to those of skill in the art. In brief, an immunogen (antigen), preferably a purified protein, a protein coupled to an appropriate carrier (e.g., GST, keyhole limpet hemanocyanin, etc.), or a protein incorporated into an immunization vector such as a recombinant vaccinia virus (see, U.S. Pat. No.
4,722,848) is mixed with an adjuvant and animals are immunized with the mixture. The animal's immune response to the immunogen preparation is monitored by taking test bleeds and determining the titer of reactivity to the protein of interest. When appropriately high titers of antibody to the immunogen are obtained, blood is collected from the animal and antisera are prepared.

Further fractionation of the antisera to enrich for antibodies reactive to the protein is performed where desired (See, e.g., Coligan, Current Protocols in Immunoloay, Wiley/Greene, NY (1991 ); and Harlow and Lane, Antibodies: A
Laboratory Manual, Cold Spring Harbor Press, NY (1989)).
2. Western Blotting of Tissue Samples for the variant D-AKAP2 protein Biological samples are homogenized in SDS-PAGE sample buffer (50 mM
Tris-HCI, pH 6.8, 100 mM dithiothreitol, 2% SDS, 0.1 % bromophenol blue, 10%
glycerol), heated at 100°C for 10 min and run on a 14% SDS-PAGE with a mM Tris-HCI, pH 8.3, 250 mM Glycine, 0.1 % SDS running buffer. The proteins are electrophoretically transferred to nitrocellulose in a transfer buffer containing 39 mM glycine, 48 mM Tris-HCI, pH 8.3, 0.037% SDS, 20% methanol. The nitrocellulose is dried at room temperature for 60 min and then blocked with a phosphate-buffered saline (PBS) solution containing either bovine serum albumin or 5% nonfat dried milk for 2 hours at 4°C.
The filter is placed in a heat-sealable plastic bag containing a solution of 5% nonfat dried milk in PBS with a 1 :100 to 1 :2000 dilution of affinity purified anti-AICAP peptide antibodies, incubated at 4°C for 2 hours, followed by three 10 min washes in PBS. An alkaline phosphatase conjugated secondary antibody (i.e., anti-mouse/rabbit IgG), is added at a 1 :200 to 1 :2000 dilution to the filter in a 150 mM NaCI, 50 mM Tris-HCI, pH 7.5 buffer and incubated for 1 h at room temperature.
The bands are visualized upon the addition and development of a chromogenic substrate such as 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT). The filter is incubated in the solution at room temperature until the bands develop to the desired intensity. Molecular mass determination is made based upon the mobility of pre-stained molecular weight standards (Rainbow Markers, Amersham, Arlington Heights, III.).
3. Microparticle Enzyme Immunoassay (MEIA) Variant D-AKAP2 protein species and peptides are detected using a standard commercialized antigen competition EIA assay or polyclonal antibody sandwich EIA assay on the IMx.RTM Analyzer (Abbott Laboratories, Abbott Park, III.). Samples containing the D-AKAP2 protein are incubated in the presence of anti-D-AKAP2 coated microparticles. The microparticles are washed and secondary polyclonal anti-D-AKAP2 antibodies conjugated with detectable entities (i.e., alkaline phosphatase) are added and incubated with the microparticles. The microparticles are washed and the bound antibody/antigen/antibody complexes are detected by adding a substrate (i.e., methyl umbelliferyl phosphate) (MUP) that will react with the secondary conjugated antibody to generate a detectable signal.
4. Immunocytochemistry Intracellular localization of the variant D-AKAP2 protein species can be determined by a variety of in situ hybridization techniques. In one method cells are fixed with fixed in 4% paraformaldehyde in 0.1 M PBS; pH7.4 for 5 min., rinsed in PBS for 2 min., dilapidated and dehydrated in an ethanol series (50, and 95%) (5 min. each and stored in 95% ethanol at 4°C).
The cells are stained with the primary anti-D-AKAP2 antibody and a mixture of secondary antibodies used for detection. Laser-scanning confocal microscopy is performed to localize the D-AKAP2 protein.
J. Biological Assays Assays to measure the interaction between the variant D-AKAP2 protein species and variant peptides provided herein and the regulatory subunits RI
and/or RII of the Protein Kinase A holoenzyme include immobilized binding assays, solution binding assays and the like. In some instances, it may be desirable to monitor binding between the variant D-AKAP2 protein species and variant peptides, and PKA. In other instances, it may be desirable to specifically monitor the binding between the variant D-AKAP2 protein species and variant peptides, and a cellular component (other than PKA) to which it binds. Assays may be performed in a variety of formats, including cell-based assays, such as di-hybrid screening or complementation assays as described in U.S. Pat. No.
5,283,173 and Patent Cooperation Treaty (PCT) Publication No. W091/16457, respectively. Assays of this type are particularly useful for assessing intracellular efficacy of test compounds. Non-cell-based assays include scintillation proximity assays, cAMP competition assays, ELISA assays, radioimmunoassays, chemiluminescent assays, and the like. Such assay procedures are well known in the art and generally described, e.g., in Boudet et a/. (1991 ) J. lmmunol. Meth., 142:73-82; Ngai et al. (1993) J. lmmunoL Meth., 158:267-276; Pruslin et al. (1991 ) J. lmmunol. Meth., 137:27-35; Udenfriend et a/ (1985) Proc. Nat/. Acad. Sci. USA, 82:8672-8676; Udenfriend et al. (1987) Ana/. Biochem., 161 :494-500; Bosworth and Towers, (1989) Nature, 341 :167-168; Gilman, (1970) Proc. Nat/. Acad. Sci. USA, 67:305-312; and U.S. Pat. No.
4,568,649.
1. In vitro binding assay Huang et al. (1997) Proc. Nat/. Acad. Sci. USA, 272:8057-8064;
Protein preparations containing D-AKAP2 fused to GST are incubated with glutathione resin in PBS for 2 hours at 4°C with 0.1 % Triton X-100, 1 mM
phenylmethylsulfonyl fluoride, 1 mM EDTA, 5mM benzamidine, and 5mM B-mercapthoethanol and washed extensively with the same buffer. 200 micrograms of PKA regulatory subunit RII and/or RI were added to the resin and incubated at 4°C. Proteins associated with the D-AKAP2 are eluted and analyzed by Laemmli electrophoresis. The proteins were visualized by Coomassie Staining. PKA proteins can be radiolabeled or labeled with a flurophore to allow detection; or can be assayed for phosphorylation activity as set forth in the next section.
2. PKA phosphorylation of protein substrate Cyclic AMP-dependent protein kinase (PKA) catalyzes the transfer of gamma phosphate from adenosine triphosphate (ATP) to a serine or threonine residue in a protein substrate. A short synthetic peptide (Leucine-Arg-Arg-Alanine-Serine-Leucine-Glycine or LRRASLG) is used as a substrate to assay the specific type of PKA activity as described in Pearson et al.
(1991 ) Meth. Enzymol., 200:62-81 .
The PKA assay is typically carried out in a reaction of the enzyme with a peptide substrate and gamma 3zP-ATP followed by separation of the 3zP-peptide product from the unreacted gamma 32P-ATP on a phosphocellulose membrane.
This method requires at least one basic amino acid residue in the peptide substrate. The peptide substrate can be tagged with a biotin group so that the biotinylated 3aP-peptide product consistently binds to a streptavidin membrane in a manner independent of the peptide sequence as described in Goueli et al.
(1995) Ana/. Biochem,, 225:10-17. The separation of the 32P-peptide product from the free gamma 32P-ATP using affinity binding and ultrafiltration separation to analyze a mixture sample as described in U.S. Pat. No. 5,869,275.
K. Screening assays for modulators Modulators of D-AKAP2 biological activities may be identified by using any of the disclosed methods related to D-AKAP2 binding to PKA, D-AKAP2 localization in the mitochondria, binding to other signaling enzymes and phosphorylation by PKA. D-AKAP2 proteins are involved in signal transduction as they bind to protein kinase A (PKA) and are thought to anchor the kinase at a location, e.g., the mitochondria, where PKA acts to phosphorylate a specific substrate, either at the mitochondria or at an ion channel. Thus, an alteration in D-AKAP2 binding to PKA, localization to the mitochondria, or phosphorylation by PKA, among other steps, will result in an alteration in signal transduction.
Accordingly, provided herein are methods of screening for agents that decrease or disrupt the binding of a Val(646) variant of D-AKAP2 with Rla PKA, comprising combining a candidate agent with a cell comprising a nucleotide sequence which encodes a Val(646) variant D-AKAP protein corresponding to SEQ ID N0:65, operably linked to a promoter such that the nucleotide sequence is expressed as a D-AKAP2 protein in the cell; and determining the effect of the agent upon the localization of PKA to the mitochondria, wherein a decrease in localization to the mitochondria identifies an agent that decreases the binding of a Val(646) variant of D-AKAP2 with Rla PKA. Also provided are high-throughput methods of screening for agents that decrease (or disrupt) the binding of a Val(646) variant of D-AKAP2 to an Rla subunit of PKA, comprising combining a candidate agent with an admixture comprising Rla and a D-AKAP2 peptide sequence that binds to Rla; and determining the effect of the agent upon the localization of PKA to a mitochondria. The candidate agent can be combined with the admixture in a cell-free system. The candidate agent is combined with the admixture intracellularly. The peptide sequence can be any of the D-AKAP2 peptides described herein.

In particular, once a variant D-AKAP2 proteins species or variant D-AKAP
peptide provided herein is contacted with a potential modulating molecule the effect of the molecule on the binding between AKAP protein or peptide and PKA
can be determined using the assays disclosed herein. For example mitochondria can be isolated from cells exposed to the potential modulating molecule. PKA
protein can then be isolated and quantitated or phosphorylation can be determined using the disclosed PKA assay. An increase in the amount of PKA
protein in the mitochondria or the quantity of test peptide phosphorylated by mitochondrial isolated PKA would indicate a positive effect of the test molecule.
Binding of the particular variant D-AKAP2 protein species, or peptide fragment thereof, and PKA could be directly assessed using an in vitro binding assay, or other disclosed binding assays such as set forth in Example 3 herein, or by immunoassays such as immunoprecipitation.
L. Assay formats and selection of test substances that modulate at least one D-AKAP2-mediated activity of a PKA holoenzyme Methods for identifying agents that modulate at least one D-AKAP2-mediated activity of a PKA holoenzyme are provided. The methods include phage display and other methods for assessing alterations in the activity of a D-AKAP2 protein and/or a PKA holoenzyme. Such methods or assays can use any means of monitoring or detecting the desired activity. A variety of formats and detection protocols are known for performing screening assays. Any such formats and protocols can be adapted for identifying modulators of D-AKAP2-mediated PKA holoenzyme activities. The following includes a discussion of exemplary protocols.
1. High throughput screening assays Although the above-described assay can be conducted where a single D-AKAP2 protein or peptide and/or PKA holoenzyme is screened, and/or a single test substance is screened in one assay, the assay typically is conducted in a high throughput screening mode, i.e., a plurality of the PKA holoenzymes are screened against and/or a plurality of the test substances are screened simultaneously (See, generally, Hiqh Throughput Screening: The Discovery of Bioactive Substances (Devlin, Ed.) Marcel Dekker, 1997; Sittampalam et al.

(1997) Curr. Opin, Chem, Biol., 1:384-91; and Silverman et al, (1998) Curr.
Opin. Chem. Biol., 2:397-403). For example, the assay can be conducted in a multi-well (e.g., 24-, 48-, 96-, 384-, 1536-well or higher density), chip or array format.
High-throughput screening (HTS) is the process of testing a large number of diverse chemical structures against disease targets to identify "hits"
(Sittampalam -et al. (1997) Curr. Opin. Chem. Biol., 1 :384-391 ). Current state-of-the-art HTS operations are highly automated and computerized to handle sample preparation, assay procedures and the subsequent processing of large volumes of data.
Detection technologies employed in high-throughput screens depend on the type of biochemical pathway being investigated (Sittampalam et al. (1997) Curr. Opin. Chem. Biol., 1 :384-391 ). These methods include, radiochemical methods, such as the scintillation proximity assays (SPA), which can be adapted to a variety of enzyme assays (Lerner -et al. ( 1996) J. Biomol. Screening, 1:135-143; Baleer et al. (1996) Ana/. Biochem., 239:20-24; Baum et al. (1996) Ana/.
Biochem.; 237:129-134; and Sullivan et al. (1997) J. Biomol. Screening, 2:19-23) and protein-protein interaction assays (Braunwalder et al. (1996) J.
Biomol.
Screening, 1:23-26); Sonatore et al. (1996) Ana/. Biochem. 240:289-297; and Chen et al. (1996) J. Biol. Chem., 271 :25308-25315), and non-isotopic detection methods, including but are not limited to, colorimetric and luminescence detection methods, resonance energy transfer (RET) methods, time-resolved fluorescence (HTRF) methods, cell-based fluorescence assays, such as fluorescence resonance energy transfer (FRET) procedures (see, e.g., Gonzalez et al, (19,95) Biophys. J., 69:1272-1280), fluorescence polarization or anisotropy methods (see, e.g., Jameson et al. (1995) Methods Enzymol., 246:283-300; Jolley, (1996) J. Biomol. Screening, 1 :33-38; Lynch et al.
(1997) Ana/. Biochem. 247:77-82), fluorescence correlation spectroscopy (FCS) and other such methods.
2. Test Substances Test compounds, including small molecules, antibodies, proteins, nucleic acids, peptides, and libraries and collections thereof, can be screened in the above-described assays and assays described below to identify compounds that modulate the D-AKAP2-mediated activity of a PKA holoenzyme. Rational drug design methodologies that rely on computational chemistry can be used to screen and identify candidate compounds.
The compounds identified by the screening methods include inhibitors, such antagonists, and can be agonists. Compounds for screening include any compounds and collections of compounds available, known or that can be prepared.
a. Selection of Compounds Compounds can be selected for their potency and selectivity of modulating either the phosphorylation activity of a PKA holoenzyme or the translocation (e.g., localization to mitochondria) of the PKA holoenzyme. As described herein, and as generally known, a variant D-AKAP2 protein species, or peptide fragment thereof (e.g,, SEQ ID NOs:17-54, and the like), a target PKA
holoenzyme and its substrate are combined under assay conditions permitting reaction of the enzyme with its substrate. The assay is performed in the absence of test compound, and in the presence of increasing concentrations of the test compound. In addition, to identify test compounds that modulate D-AKAP2 mediated PKA activity, these assays can be performed in the absence of D-AKAP2, or fragments thereof. Those of skill in the art will understand that if a test compound demonstrates modulating activity of PKA in the presence of D-AKAP2, or fragments therof, and not in its absence, then that compound is identified and selected as a D-AKAP2 mediated modulator of PKA activity. The concentration of test compound at which 50% of the enzymatic activity (e,g., phosphorylation activity) is inhibited by the test compound is the ICSO value (Inhibitory Concentration) or ECSO (Effective Concentration) value for that compound. Within a series or group of test compounds, those having lower ICSo or ECSO values are considered more potent inhibitors of the PKA enzymatic activity than those compounds having higher ICSO or EC5° values. The ICSo measurement is often used for more simplistic assays, whereas the ECSO is often used for more complicated assays, such as those employing cells.

Typically candidate compounds have an ICSO value of 100 nM or less as measured in an in vitro assay for inhibition of PKA holoenzyme activity. The test compounds also are evaluated for selectivity toward a particular isoform of PKA, such as an Rla or and Rlla containing PKA. As described herein, and as generally known, a test compound is assayed for its potency toward a panel of variant D-AKAP2 protein species, or peptide fragments thereof (e.g., SEQ ID
NOs:17-54, and the like) and/or a target PKA holoenzyme, and other enzymes and an ICSO value or ECSO value is determined for each test compound in each assay system. A compound that demonstrates a low ICSO value or ECSO value for the target enzyme, e.g., PKA holoenzyme, and a higher ICSO value or ECSO value for other enzymes within the test panel (e.g., urokinase tissue plasminogen activator, thrombin, Factor Xa), is considered to be selective toward the target enzyme. Generally, a compound is deemed selective if its ICSO value or ECSo value in the target enzyme assay is at least one order of magnitude less than the next smallest ICSO value or ECSO value measured in the selectivity panel of enzymes.
Compounds are also evaluated for their activity in vivo. The type of assay chosen for evaluation of test compounds depends on the pathological condition to be treated or prevented by use of the compound, as well as the route of administration to be evaluated for the test compound.
The following examples are included for illustrative purposes only and are not intended to limit the scope of the invention. The practice of methods and development of the products provided herein employ, unless otherwise indicated, conventional techniques of cell biology, cell culture, molecular biology, transgenic biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature.
See, for example, Molecular Cloning A Laboratory Manual, 2nd Ed., ed, by Sambrook, Fritsch and Maniatis (Cold Spring Harbor Laboratory Press: 1989);
DNA Cloning, Volumes I and II (D.N. Glover, ed., 1985); Oligonucleotide Synthesis (M.J. Gait, ed., 1984); Mullis et ai., U.S. Pat. No. 4,683,195;
Nucleic Acid Hybridization (B.D. Hames & S.J. Higgins, eds., 1984); Transcription and Translation (B.D. Hames & S.J. Higgins, eds., 1984); Culture of Animal Cells (R.I. Freshney, Alan R. Liss, Inc., 1987); Immobilized Cells and Enzymes ,(IRL
Press, 1986); B. Perbal, A Practical Guide To Molecular Cloning (1984); the treatise, Methods In Enzymology (Academic Press, Inc., New York); Gene Transfer Vectors For Mammalian Cells (J.H. Miller and M.P. Calos, eds., 1987, Cold Spring Harbor Laboratory); Methods In Enzymoloay, Vols. 154 and 155 (Wu et al., eds.); Immunochemical Methods In Cell and Molecular Bioloay (Mayer and Walker, eds., Academic Press, London, 1987); Handbook of Experimental Immunology, Volumes I-IV (D.M. Weir and C.C. Blackwell, eds., 1986);
Manipulating the Mouse Embryo, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986).

Assay of the Binding of D-AKAP2, A-Kinase Binding (AKB) domain Ile/Val variants to PKA.
The variable amino acid (Ile/Val) is located at amino acid 646 of SEQ ID
NOs:64 and 65 in the AKB domain of D-AKAP2. This domain is the docking site for PKA and is highly conserved among species. Similar to other AKAPs, the AKB domain forms an amphipathic helix with hydrophobic amino acids on one face of the helix. To determine whether the D-AKAP2 Ile/Val variation resulted in an alteration in its binding properties to PKA, the binding of each variant to the regulatory subunit of PKA was investigated.
Binding of each AKB domain variant to PKA was first examined using an in vitro pull-down assay, in which the 40 C-terminal residues of D-AKAP2 (amino acids 623-662 of SEQ ID N0:64) containing the AKB were fused to glutathione-S-transferase (GST). The GST fusion constructs were made by fusing the 40 C-terminal amino acids of D-AKAP2 to the C-terminus of GST and subcloning between the Ndel and BamHl sites of pRSET (Invitrogen, Carlsbad, CA). The constructs were transfected into BL21 cells and expressed for 6 hours at 20°C.
The cells were lysed in PBS with 5mM BME and 0.1 % Triton X-100. 3 NI of supernatant were added to 200 ,ul of this buffer and 1 O,ul glutathione beads.
After three washes, Rla (53 ,ug, 20 ,uM) and Rlla (2.4 Ng, 2 NM) were added to the beads, respectively, and the total volume was adjusted to 40 ,ul. After incubating for 30 min at 4°C, the beads were washed three times, and separated in a 10% acrylamide gel.
The Ile/Val substitution resulted in an isoform specific difference in PKA
binding. The Rla isoform of PKA bound with a significantly higher affinity to the Val variant. The higher affinity was seen for both the mouse and the human AKB domains. The residue differences between mouse and human downstream of the Ile/Val position had no effect on the binding properties. Moreover, there was no difference in binding of the variants to the Rlla isoform.

Assay of the Binding Affinity of each Ile/Val AKB domain variant to PKA R-subunit isoforms.
To assess the magnitude of the affinity difference, binding of both Ile/Val variants to the R-subunit isoforms of PKA was analyzed in a quantitative assay.
Twenty-seven residue peptides containing the two AKB domain Ile/Val variants (SEQ ID NOs:1 and 2, respectively) of D-AKAP2 with a C-terminal cysteine were synthesized by SynPep Corporation (Dublin, CA). The peptides were HPLC (high performance liquid chromatography) purified and mass spectrometry checked.
Both peptides were labeled with tetramethyl rhodamine-5-maleiamide (Molecular Probes, Eugene, OR) at the cysteine residue and HPLC purified. Increasing concentrations of Rla and R11~3, respectively, were equilibrated with 10nM of peptide for at least one hour at room temperature in 10mM HEPES, 150mM
NaCI, 3mM EDTA, 0.005% polysorbate 20. For Rlla tests, 1 nM of labeled peptide was used since the binding affinity to Rlla was higher than to Rla and RI1~3. Fluorescence anisotropy was monitored using a Fluoromax-2 (Spex, Jobin Yvon Horiba, Edison, NJ) equipped with polarizers. The fluorophore was excited at 541 nm (5-10nm bandpass) and emission was monitored at 575nm (5-1 Onm bandpass). For each protein three separate binding experiments were averaged and fit to a 1 :1 binding model using the non-linear regression application in GraphPad Prism version 3.00 (GraphPad Software, San Diego, CA).
Peptides of each AKB Ile/Val variant (SEQ ID NOs:1 and 2) were synthesized and fluorescence-labeled. Binding of the labeled peptides to the regulatory subunit isoforms was monitored using a fluorescence assay in which

-7 5-binding of the peptide was proportional to an increase in steady state anisotropy.
There was no difference in binding of the AKB variants to either the Rlla or RII,Q
isoforms. In addition, the RII isoforms bound tighter to the variants than the R1 a isoform. However, as indicted by the pull-down experiments, R1 a displayed differential binding to the AKB variants. The Val variant (SEQ ID N0:2) had a nearly three-fold increase in binding affinity when compared to the Ile variant (SEQ. ID N0:1 ). The interaction was specific since no binding was observed to a deletion construct of Rla that lacked the AKAP binding domain (delta 1-91 Rla).

/n vivo assays of the association of the AI(B IIe646Va1 variants and the PKA
Regulatory subunits and targeting to mitochondria.
To determine whether the observed in vitro affinity differences resulted in a difference in cellular compartmentalization, the association of the AKB
Ile/Val variants and the PKA regulatory subunits in vivo was examined. The 30 amino acid mitochondrial anchoring domain of D-AKAP1 (Chen et al. (1997) J. Bioi.
Chem., 272:15247-57; and Huang et al. (1997) J. Biol. Chem., 272:8057-8064) was fused to the C-terminal 156 residues of mouse (amino acids 217-372 of SEQ ID N0:55) and human (amino acids 507-662 of SEQ ID N0:64) D-AKAP2, respectively, followed by a Flag-tag and subcloned into pcDNA4 (Invitrogen, Carlsbad, CA). The dimerization/docking (D/D) domain of Rla and full-length Rlla were each fused with green fluorescent protein (GFP) and cloned into pEGFPN1 (Clontech, Palo Alto, CA). Equal molar ratios of the Flag-tagged D-AKAP2 constructs and either Rlla-GFP or Rla-D/D-GFP constructs were mixed and transfected into 10T(1/2) cells using cytofectene (BioRad, Hercules, CA).
The AKB domain was detected by immuno-staining with monoclonal antibodies against the Flag-tag (Kodak, Rochester, NY) followed by rhodamine-conjugated secondary antibody (Jackson Lab, Bar Harbor, ME). The cells were imaged using a Zeiss microscope equipped with a digital camera. Each channel was exposed for the same amount of time.
In this assay, the AKB domain within the C-terminal 156 residues of D-AKAP2 from mouse (Val) and human (Ile) was fused to the mitochondrial anchoring domain from D-AKAP1 and tethered to the outer mitochondrial membrane (Chen et al. (1997) J, Biol, Chem., 272:15247-15257; and Huang et a/. (1997) J. Bioh Chem., 272:8057-8064). The binding of the AKB domain with the PKA regulatory domains was detected as the co-transfected PKA
regulatory domains co-localized to the mitochondria. Both the human and mouse AKB domains can target Rlla to the mitochondria effectively, in accordance with their similar affinity in vitro. However, for R1 a there was a difference in co-localization between the variants. The Val variant effectively targeted Rla to the mitochondria. The Ile variant, however, was unable to target Rla, which was evenly diffused in the cytosol suggesting that the Ile variant of D-AKAP2 may have impaired ability to sequester Rla.
The D-AKAP2 variants at amino acid 646 of SEQ ID NOs:64 and 65 described herein map to the conserved AKB domain of D-AKAP2, which was previously shown to interact with the regulatory subunit of PKA. In accordance with the methods provided herein, it has been demonstrated that this variation impacts the binding to PKA in an isoform specific manner both in vitro and in vivo. The Val(646) variant at amino acid 646 of SEQ ID N0:65, which has previously been identified as a deleterious allele associated with morbidity in the age-stratified approach (see, e.g., US Patent Application US20020040130A1 and PCT WO 02/04489), binds three-fold tighter to the Rla isoform when compared to the Ile(646) variant. At the cellular level, this affinity difference resulted in a decrease in mitochondrial localization of the Ile (646) variant.

Development of a detection system to verify binding of 27-mer peptides to PKA
homodimers.
A peptide SPOT-synthesis technique was applied to study the interaction of a 27-mer D-AKAP2-derived peptide VQGNTDEAQEELAWKIAKMIVSDIMQQ
(SEQ ID N0:1 ) with the regulatory subunit PKA-Rllcr. However, prior to binding studies on cellulose membranes, the interaction of the 27-mer with PKA was confirmed by ELISA (enzyme-linked immunosorbent assay).
a) ELISA Assays The peptide biotin-bA-bA-VQGNTDEAQEELAWKIAKMIVSDIMQQ (SEQ ID
N0:14) was synthesized as a C-terminal amide in milligram quantity and purified _77_ to a 96% purity by HPLC. Its identity was confirmed by MALDI TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry. The peptide was immobilized in neutravidin (NA)-coated 96-well microtiter plates and incubated with various concentrations of PKA-Rlla-GFP. Binding was detected using an anti-GFP antibody (3E6, Quantum Biotechnologies, QBiogene, Carlsbad, CA) in combination with a second horseradish peroxidase labeled antibody.
Microtiterplates were coated with 40 pg/ml Neutravidin (NA) and subsequently incubated with 25 ~rM peptide. Various concentrations (0-5 ,ug/ml) of PKA-Rlla-GFP were added. Bound subunit was detected using a mouse anti-GFP antibody (1 pg/ml) and an anti-mouse-IgG-antibody labeled with peroxidase (1 ~ug/ml).
Negative controls were utilized that contained either a microtiter plate with only neutravidine or only peptide, or neither one. The only signal detected was in wells containing both neutravidin and peptide, indicating that the 27-mer peptide corresponding to SEQ ID N0:1, when immobilized on Neutravidin coated wells, is able to bind to PKA-Rlla in the same experiment.

Assay of binding properties of the 27-mer peptide dependent upon amino acid sequence composition and length.
a) Identification of critical residues for binding PKA within the 27-mer peptide (SEQ ID N0~1) corresponding to the binding domain of D-AKAP2.
To identify key residues within the 27-mer Ile/Val peptides (SEQ ID N0:1 ) for interaction with PKA, an alanine, an aspartic acid, and a lysine scan was performed. The filters for the peptide arrays were prepared by SPOT-synthesis and incubated with PKA-Rlla-GFP (see, e.g., Frank R., (1992) Tetrahedron, 48 42 :9217-9232; Kramer et al. (1994) Comp. Meth. Enzymol., 6:388-395;
Kramer et al. (1997) Meth. Mol. Biol., 87:25-39; and Kramer et al. (1999) J.
Peptide Res., 54:319-327). Binding was detected using an anti-GFP antibody (3E6, Quantum Biotechnologies, QBiogene, Carlsbad, CA) in combination with a secondary antibody and a chemiluminescence-imager. All scans revealed a clear key residue pattern.
Residues of the 27-mer peptide that cannot be substituted for while retaining the ability to bind Rlla: in the Ala-scan are residues 16, 20 and 21; in _78_ the Asp-scan are residues 12, 13, 16, 17, 20, 21 and 24; in the Lys-scan are residues 12, 13, 16, 17, 20, 21, and 24 of the 27-mer peptide (SEQ ID N0:1 ).
Residues that result in reduced binding upon substitution in the Ala-scan are residues 10, 12, and 23; in the Asp-scan are residues 14 and 15; and in the Lys-scan is residue 14 of the 27-mer peptide (SEQ ID N0:1 ). These results indicate a helical structure of the binding domain. Although the variable position (Ile/Val) occurs at amino acid residue 24, in this experiment only isoleucine containing peptides were synthesized.
b) Amino- and Carboxy-terminal truncation experiments to identify minimal peptide length capable of binding to PKA-Rla and PKA-Rlla.
Several truncation analogs of the 27-mer peptide corresponding to SEQ ID
N0:1 have been synthesized and tested for binding. N-terminal truncations, C-terminal truncations, and N/C-terminal truncations were performed. For example, Table 3 sets forth N/C-terminal truncations where, sequentially, both an N- and a C-terminal amino acid were omitted in each SPOT-synthesis.

Relative ability to bind to:
PEPTIDES Rla Rlla SEQ ID NO:
VQGNTDEAQEELAWKIAKMIVSDIMQQ + + + + + SEQ ID N0:1 +

QGNTDEAQEELAWKIAKMIVSDIMQ + + + + + SEQ ID N0:5 +

GNTDEAQEELAWKIAKMIVSDIM + + + + + SEQ ID N0:6 +

NTDEAQEELAWKIAKMIVSDI + + + + + SEQ ID NOs:3 + and 7 TDEAQEELAWKIAKMIVSD -- + + SEQ ID N0:8 DEAQEELAWKIAKMIVS -- + + SEQ ID N0:9 EAQEELAWKIAKMIV -- + SEQ ID N0:4 AQEELAWKIAKMI -- -- SEQ ID N0:10 QEELAWKIAKM -- -- SEQ ID N0:11 EELAWKIAK -- -- SEQ ID N0:12 ELAWKIA -- -- SEQ ID N0:13 _79_ In this experiment, only the isoleucine variant 27-mer peptide (SEQ ID N0:1 ) was used (amino acid position of variation corresponds to position 24 of SEQ
ID
N0:1 ). Incubation with PKA-Rla-GFP and PKA-Rlla-GFP and detection with antibodies was performed as described above. It has been found that several residues from the N- and C-terminus can be omitted without significant loss of binding. The shortest peptide with no reduced signal intensity identified for both Rla and Rlla is a 21-mer with the following sequence:
NH2-NTDEAQEELAWKIAKMIVSDI-COOH (SEQ ID NOs:3 and 7).
It is important to note that the Ile/Val polymorphic amino acid position corresponding to the last residue in the above peptide SEQ ID NOs:3 and 7 (or residue 24 in SEQ, ID N0:1 ) is essential for binding to the Rla subunit. If the peptide is truncated to exclude residue 24 in SEQ ID N0:1, the remaining peptide does not bind to Rla at all. In addition, if the peptide is truncated to exclude residue 24 in SEO ID N0:1, binding to Rlla is reduced. In this particular N/C-terminal dual truncation assay, it has been found that the shortest peptide that still binds to Rlla is a 15-mer: NH2-EAQEELAWKIAKMIV-COOH (SEQ ID
N0:4).
In addition, the minimal sequence required for regulatory subunit binding was also assessed using N- and C-terminal truncations of the 27-residue human D-AKAP2 sequence. N-terminal or C-terminal truncated peptides were synthesized using SPOT synthesis on cellulose membrane as described herein.
Binding was evaluated by incubating each membrane with GFP-Rla D/D and GFP-Rlla D/D as indicated below. The dimerization/docking (D/D) domain of bovine Rla (residues 1-109) and mouse Rlla (residues 1-46), fused to green fluorescence protein (GFP) were subcloned into a pRSET expression vector (Invitrogen, Carlsbad, CA) downstream of a histidine tag. The proteins, GFP-Rla D/D and GFP-Rlla D/D were expressed in E. coli BL21 (DE3) and purified using Talon (Clontech, Palo Alto, CA) resin. The His tag was cleaved using thrombin and the protein further purified using an S75-Sephadex (16/60) gel filtration column (Pharmacia, Peapack, NJ) in 50 mM MES pH 5.8, 50 mM NaCI, 2 mM
EDTA, 2 mM EGTA, 2 mM DTT. The protein was stored at 4°C.

Bound protein was detected using a primary antibody against GFP and enzyme conjugated secondary antibody for amplification of signal. The membrane was then analyzed by chemiluminescence. The results are set forth in Tables 4 and 5.

Relative ability to bind to:
PFPTII~FS Rlla Rla SEQ ID NO:
VQGNTDEAQEELAWKIAKMIVSDIMQQ + + + + + SEQ ID N0:1 +

6Q,GNTDEAQEELAWKIAKMIVSDIMQO + + + + + SEQ ID N0:66 +

GNTDEAQEELAWKIAKMIVSDIMQQ + + + + + SEQ ID N0:67 +

NTDEAQEELAWKIAKMIVSDIMQQ + + + + + SEQ ID N0:68 +

TDEAQEELAWKIAKMIVSDIMQQ + + + + + SEQ ID N0:69 +

DEAQEELAWKIAKMIVSDIMQQ + + + + + SEQ ID N0:70 +

EAQEELAWKIAKMIVSDIMQQ + + + + + SEQ ID N0:71 AQEELAWKIAKMIVSDIMQQ + + + + + SEQ ID N0:72 O.EELAWKIAKMIVSDIMOQ + + + + + SEQ ID N0:73 EELAWKIAKMIVSDIMQQ + + + + SEQ ID N0:74 ELAWKIAKMIVSDIMQQ + + + SEQ ID N0:75 LAWKIAKMIVSDIMQQ + -- SEQ ID N0:76 AWKIAKMIVSDIMQQ + -- SEQ ID N0:77 WKIAKMIVSDIMQQ -- -- SEQ ID N0:78 KIAKMIVSDIMQQ -- -- SEQ ID N0:79 IAKMIVSDIMQQ -- -- SEQ ID N0:80 AKMIVSDIMQO -- -- SEQ ID N0:81 KMIVSDIMQQ -- -- SEQ ID N0:82 Relative ability to bind to:
PEPTIDES Rlla Rla SEQ ID N0:
VQGNTDEAQEELAWKIAKMIVSDIMQQ + + + + + SEQ ID N0:1 +

VQGNTDEAQEELAWKIAKMIVSDIMQ + + + + + SEQ ID N0:83 +

VQGNTDEAQEELAWKIAKMIVSDIM + + + + + SEQ ID N0:84 +

VQGNTDEAQEELAWKIAKMIVSDI + + + + + SEQ ID N0:85 +

VQGNTDEAQEELAWKIAKMIVSD + + -- SEQ ID N0:86 +

VQGNTDEAQEELAWKIAKMIVS + + -- SEQ ID N0:87 VQGNTDEAQEELAWKIAKMIV + + -- SEQ ID N0:88 VQGNTDEAQEELAWKIAKMI -- -- SEQ ID N0:89 VQGNTDEAQEELAWKIAKM -- -- SEQ ID N0:90 VQGNTDEAQEELAWKIAK -- -- SEQ ID N0:91 VQGNTDEAQEELAWKIA -- -- SEQ ID N0:92 VQGNTDEAQEELAWKI -- -- SEQ ID N0:93 VQGNTDEAQEELAWK -- -- SEQ ID N0:94 VQGNTDEAQEELAW -- -- SEQ ID N0:95 VQGNTDEAQEELA -- -- SEQ ID N0:96 VQGNTDEAQEEL -- -- SEQ ID N0:97 VQGNTDEAQEE -- -- SEQ ID N0:98 VQGNTDEAQE -- -- SEQ ID N0:99 The C-terminal truncations defined clearly the C-terminal boundary for binding to the isoforms. There was an absence of binding to both regulatory subunits at a defined residue from the C-terminus. For Rla, binding abruptly stopped after the C-terminal isoleucine (VQGNTDEAQEELAWKIAKMIVSDI; SEQ
ID N0:85), suggesting that the C-terminal (...MQQ) residues are not required for binding (Table 5). This C-terminal isoleucine residue is the location of a single nucleotide polymorphism of D-AKAP2, which codes for either a valine or isoleucine at this position (1646V; SEQ ID NOs:64 and 65). For Rlla, binding abruptly stopped at the upstream valine position (VQGNTDEAQEELAWKIAKMIV;
SEQ ID N0:88), suggesting that more C-terminal residues (...SDIMQQ; SEQ ID
N0:1, amino acids 22-27), which contain the polymorphism, are dispensable for the Rlla binding site (Table 5). This is consistent with the 1646V polymorphic site only having an effect on binding to the Rla isoform.
The N-terminal truncations did not result in a clear-cut boundary, but rather there was a titrateable decrease in signal for both Rla and Rlla isoforms starting at the glutamine (QEELAWKIAKMIVSDIMQQ; SEQ ID N0:73) (Table 4).
This suggests that the N-terminal negative charges play a role in enhancing the affinity to both isoforms.

Assay of the binding properties of the two naturally occurring allelic variants of the D-AKAP2 protein.
a) Optimization of peptide density and regeneration protocols.
First, the optimal peptide density for further synthesis was determined to improve quantification of binding differences between both PKA isoforms. This was achieved by synthesizing two sets of membranes containing two peptide sequences representing the Ile and Val alleles, respectively:
VQGNTDEAQEELAWKIAKMIVSDIMQQ (SEQ ID N0:1 ) VQGNTDEAQEELAWKIAKMIVSDVMQQ (SEQ ID N0:2) Both peptides were synthesized as 5 spots varying in peptide density from 50% to 0.1 % membrane saturation. Then, set 1 was incubated with PKA-Rla-GFP, set 2 with PKA-Rlla-GFP to determine the optimal peptide density for the binding assay. Subsequently, the membranes were regenerated and incubated with the other regulatory subunit, respectively (set 1 with PKA-Rlla-GFP, set 2 with PKA-Rla-GFP). This was to verify the feasibility of regenerating the membranes, which was beneficial for the following experiments as well as to identify the preferred order of incubation.
Membranes after incubation and GFP-signal development, as well as quantification of the signal intensities revealed that the signal intensity for PKA-Rla-GFP decreases with reduction of the peptide density, whereas the highest signals for PKA-Rlla-GFP are observed for reduced peptide densities between 5 and 10%. These findings correlate with a higher affinity of the peptides for PKA-Rlla-GFP. A reduced peptide density of 10% suitable for both regulatory subunits was suggested for further experiments.
For PKA-Rlla-GFP, an approximate 10-fold higher signal intensity compared to PKA-Rla-GFP was measured. Since both membranes were incubated simultaneously under the same conditions this finding must be due to the higher affinity of PKA-Rlla-GFP for the D-AKAP2-derived peptide. No significant PKA-Rlla-GFP binding differences between the Ile and Val variants of the D-AKAP2-derived peptide were observed. However, allele-specific differences were obtained for PKA-Rla-GFP.
After stripping and regeneration of membranes similar results were obtained compared to a fresh filter set. The signal intensity was about 10%
lower. However, the regeneration protocol is suitable since no signals were observed in a control experiment with the detection antibodies alone. The preferred order for further experiments was determined to be incubation of peptide membranes with PKA-Rla-GFP followed by PKA-Rlla-GFP due to the differences in signal intensity.
b) Quantification of allele-specific binding differences.
Two 27-mer peptides representing the Ile and Val variants (SEO. ID NOs:1 and 2, respectively) were synthesized and purified in milligram quantities and the affinity to PKA-Rla-GFP was determined using well-known surface plasmon resonance in combination with BIAcore chips (see, e.g., Current Opinion in Biotechno -logy (1997) 8:50-57; Current Opinion in Biotechnology (1994) 5:389-395; Current Opinion in Biotechno -logy, (1994) 5:65-71; Structure (1995) 3:969;
Current Bio%gy~(1995) pp. 699-705; Analytical Biochemistry (1991) 201:197-210; and macinfac.bio.unc.edu/biacore.html). PKA-Rla-GFP was coupled to BIAcore CM5 chips. BSA was immobilized on control chips.
Chip 1 : PKA-Rla-GFP (1681 RU) Chip 2: BSA (1424 RU) PKA-Rla-GFP binding affinity PKA-Rla-GFP

Ac-VQGNTDEAQEELAWKIAKMIVSDVMQQ-NHz 5.0 x 10-' SEQ ID
N0:15 Ac-VQGNTDEAQEELAWKIA KMIVSDIMQO-NHa8.6 x 10-' SEQ ID
N0:16 The affinities in this assay are lower compared to those determined by fluorescence anisotropy. The most likely reason is that in this study the signal difference between the flow cell with the regulatory subunit and the control protein BSA was measured, such that slight unspecific binding to BSA directly reduces the apparent affinity. However, similar to previous results, an affinity difference of PKA-Rla-GFP to both allelic 27-mer peptides was observed (Table 6).

Identification of substituted peptide sequences that are able to disrupt the interaction between D-AKAP2 and PKA completely by either binding specifically PKA-Rla- or -Rlla or by binding both PKA isoforms.
Substitution analysis of both allelic peptides (VQGNTDEAQEELAWKIAKMIVSDVMQQ; SEQ ID N0:2 and VQGNTDEAQEELAWKIAKMIVSDIMQQ; SEQ ID N0:1 ) was conducted. Each amino acid of the 27-mer peptide was substituted, one amino acid at a time per peptide, by any naturally occurring L- and D- amino acid. For both peptides complete L- and D- substitution analysis membranes have been screened for binding of PKA-Rla-GFP. After regeneration, the peptide arrays were incubated with PKA-Rlla-GFP. After binding, detection was carried out with an anti-GFP
antibody in combination with a peroxidase-labeled secondary antibody.
a) Peptide Array Synthesis The cellulose-bound peptide libraries were automatically prepared according to standard SPOT synthesis protocols (Frank, R., (1992) Tetrahedron, 48:9217-9232) using a SPOT synthesizer (Abimed GmbH, Langenfeld, Germany) as described in Kramer et al. (1998) Methods Mol. Biol., 87:25-39 and Wenschuh et al. (2000) Biopolymers, 55:188-206. The peptides were synthesized on an amino functionalized cellulose membrane as distinct spots. A
/3-alanine dipeptide spacer was inserted between the C-terminus of the peptide and the membrane support. The peptide loading of the membranes was reduced by mixing 10% Fmoc-~3-alanine-OPfp and 90% acetylated ~3-alanine-OPfp active esters for the first coupling step. This peptide loading was optimized in advance by varying the Fmoc-~3-alanine-OPfp percentage from 0.1 % to 50%. The peptide was extended stepwise using standard Fmoc solid-phase peptide synthesis followed by cleavage of the side chain protecting groups under trifluoroacetic acid (TFA) conditions. Sequence files were generated with the software DIGEN
(Jerini AG, Berlin, Germany). All peptides were N-terminally acetylated. For synthesis quality control, a selection of peptides that was synthesized in duplicate was cleaved from the solid support by ammonia vapor in the dry state.
Subsequently, identity was verified by MALDI-MS (Voyager-DE, Applied Biosystems, Foster City, CA, USA).
b) Peptide Array Screening The peptide arrays were pre-incubated with T-TBS blocking buffer (TBS
pH 8.0, 0.05% Tween 20 in the presence of blocking reagent; Roche Diagnostics Chemiluminescence detection kit 1500694, Mannheim, Germany).
Subsequently, the peptide arrays were incubated with solutions of GFP-Rla D/D
or GFP-Rlla D/D at a final concentration of 1 .0 Ng/ml for 2 h in T-TBS
blocking buffer. After washing three times for 10 min with T-TBS the anti-GFP antibody 3E6 (Quantum Biotechnologies, QBiogene, Carlsbad, CA) was added to a final concentration of 1 Ng/ml in T-TBS blocking buffer for 1 h followed by washing three times for 10 min with T-TBS. Finally, the arrays were incubated with a second anti-mouse IgG peroxidase-labeled antibody (Catalog #: A5906, Sigma, Deisenhofen, Germany) which was applied at a concentration of 1 ,ug/ml in T-TBS blocking buffer for 1 h, followed by washing three times for 10 min with T-TBS. Analysis and quantification of peptide-bound GFP-Rla D/D- or GFP-Rlla D/D-antibody complexes was done using a chemiluminescence substrate and the LumilmagerT"" (Roche Diagnostics, Mannheim, Germany). All steps were carried out at room temperature. Binding of the detection antibodies to the peptides was excluded by control incubations with antibody 3E6 and the anti-mouse IgG

peroxidase-labeled antibody alone. For a given membrane, binding of GFP-Rla D/D was performed first. Subsequently, the membrane was regenerated using detergent (Kramer -et al. (1998) Methods Mol. Biol., 87:25-39) and complete removal of the GFP-Rla D/D was proven by a control incubation with antibody 3E6 and the anti-mouse IgG peroxidase-labeled antibody alone. Afterwards, binding of GFP-RII D/D to the peptide array was assessed.
The results of the incubated membranes containing the single amino acid substitutions using L-amino acids are shown in Figure 1 . Figure 1 shows the amino acid substitution analysis of both isoforms (V/124; SEQ ID NOs:1 and 2:
of a 27-mer D-AKAP2 peptide that is the binding domain for the Rla and Rlla subunits of protein kinase A (PKA). Each amino acid of the respective D-AKAP2 peptide (vertical lane) was substituted, one amino acid at a time, by a single L-amino acid (horizontal line). The top filters show the results of substituting amino acid residues in the Valine variant (SEQ ID N0:2; and the bottom filters represent the substitutions in the Isoleucine variant (SEQ ID N0:1 ). The left hand filters were analyzed with PKA-Rla-GFP and right hand filters with PKA-Rlla-GFP, respectively. Key amino acid residues for the binding to both Rla and Rlla are indicated to be residues 12, 13, 16, 17, 20 and 21 . The vertical dark boxes occurring at residues 10-21 of the V/124 peptides in the proline column indicate loss of binding to both isoforms after proline substitution in the middle part of the peptide. The light colored boxes mark substitutions specifically disrupting or significantly decreasing the binding to PKA-Rla-GFP.
Substitutions that significantly enhance the binding of both V/124 peptides to PKA-Rla-GFP
are dark-circled and correspond to the substitution in SEQ ID NOs:1 and 2 of one, two or more: of Q at residue 9 with F, I, L, V, H, M, R, T, W or Y; of L at residue 12 with F, W or Y; of V at residue 21 with I, L or W; and of M at residue 25 with F, I, L, T, V, W or Y. For light-colored boxes and circled markings, the binding to PKA-Rlla-GFP can be either increased, normal, decreased or even completely disrupted.
Key residues for binding of D-AKAP2 peptide variants to PKA-Rla-GFP
and PKA-Rlla-GFP are 116, A17, 120, and V21 . For binding to both PKA-GFP
subunits (Rla and Rlla) these critical residues cannot be substituted on the _87_ peptide without loss of binding except by physicochemically very similar ones, which will lead to reduced binding (e.g., A17 by I or V). The substitution pattern reflects the postulated helical binding structure of the peptide. Key residues are found on one side of the helical wheel and proline substitutions in the central part of the peptide corresponding to residues 10-21 are disadvantageous for the binding to both isoforms of PKA.
In this substitution analysis only slight differences between the valine and isoleucine variants were observed. However, it was not the objective to compare both allelic peptides quantitatively.
D-amino acid substitutions in the central part of the peptide reduce the binding ability to both PKA subunits. The binding to PKA-Rlla-GFP was regularly decreased throughout the entire peptide for all substitutions. There were a few substitutions, which enhanced the binding to PKA-Rla-GFP in the context of all other substitutions. The following list indicates those peptides and substitutions with D-amino acid analogs in underlined and bold one-letter code.
VQGNTTEAQEELAWKIAKMIVSD[I/VIMQQ;(SEQ ID N0:17) VQGNTDEAFEELAWKIAKMIVSD[I/V]MQQ;(SEQ ID N0:18) VQGNTDEAIEELAWKIAKMIVSD[I/V]MQQ;(SEQ ID N0:19) VQGNTDEALEELAWKIAKMIVSD[I/V]MQQ;(SEQ ID N0:20) VQGNTDEAVEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:21 ) VQGNTDEAWEELAWKIAKMIVSD[I/V]MQQ;(SEQ ID N0:22) VQGNTDEAYEELAWKIAKMIVSD[I/V]MQQ;(SEQ ID N0:23) VQGNTDEAQEELAWKIAKMILSD[I/V]MQQ;(SEQ ID N0:24) VQGNTDEAQEELAWKIAKMIVLD[I/V]MQQ;(SEQ ID N0:25) and VQGNTDEAQEELAWKIAKMIVSD[I/V]FQQ. (SEQ ID N0:26) Identification of peptide sequences that show preferential and/or enhanced binding to either PKA-Rla or -Rlla.
As a result of the substitution analysis conducted as described in Example 7 and set forth in Figure 1, it has been found that amino acid residues L12 and A13 are key residues for PKA-Rla binding but not for PKA-Rlla binding, which _88_ indicates that binding of the 27-mer peptides to PKA-Rla-GFP is more entailed compared to PKA-Rlla-GFP. Also residues D23 and V1124 (corresponding to the polymorphic position within the 27-mer peptides), are residues with a limited preference for certain amino acids regarding the binding to PKA-Rla-GFP.
Exemplary substitution analogs leading to preferred or exclusive binding of the 27-mer peptides to PKA-Rla-GFP relative to PKA-Rlla-GFP; or enhanced binding to both Rla and Rlla isoforms correspond to the substitution in SEQ ID
NOs:1 andlor 2 of one, two or more: of Q at residue 9 with F, I, L, V, H, M, R, T, W or Y; of L at residue 12 with F, W or Y; of V at residue 21 with I, L or W;
and of M at residue 25 with F, I, L, T, V, W or Y.
For the 27-mer Val-isoform (SEQ ID N0:2), exemplary substitution analogs leading to preferred or exclusive binding to PKA-Rlla-GFP relative to PKA-Rla-GFP correspond to the substitution in SEQ ID N0:2: of L at residue 12 with A, C, or K; of A at residue 13 with F, H, I, K, L, M or N; of W at residue 14 with C; of K at residue 15 with C; of K at residue 18 with C; of M at residue with C; of S at residue 22 with C; and of D at residue 23 with C.
For the 27-mer Ile-isoform (SEQ ID N0:1 ), exemplary substitution analogs leading to a preferred or exclusive binding to PKA-Rlla-GFP relative to PKA-Rla-GFP correspond to the substitution in SEQ ID N0:1; of A at residue 13 with F, H, I, L, M and S; of W at residue 14 with C; of K at residue 15 with C; of K
at residue 18 with C; of M at residue 19 with C; of S at residue 22 with C; and of D at residue 23 with C.
The following is an exemplary list of peptides substituted with L- and D-amino acid residues bearing specific novel binding properties. Since the design of these experiments was not done to disclose binding differences between the two allelic peptides (SEQ ID NOs:1 and 2), both peptide syntheses can be used as mutual replications. Peptides that show similar binding properties in two independent experiments are indicated below.
A. Peptides with substantially no binding to PKA-Rla-GFP but normal binding to PKA- -Rlla-GFP Substituted L-amino acids are indicated in bold and the polymorphic site in brackets:

_89_ VQGNTDEAQEELFWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:27) VQGNTDEAQEELIWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:28) VQGNTDEAQEELLWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:29) VQGNTDEAO.EELAWCIAKMIVSD[I/V]MQQ; (SEQ ID N0:30) VQGNTDEAQEELAWKIACMIVSD[I/V]MQQ; (SEQ ID N0:31 ) VQGNTDEAQEELAWKIAKCIVSD[I/V]MQQ; (SEQ ID N0:32) and VQGNTDEAQEELAWKIAKMIVCD[I/V]MQQ. (SEQ ID N0:33) B. _Peptides with substantially no binding to PKA-Rla-GFP and reduced binding to PKA-Rlla-GFP Substituted L-amino acids are indicated in bold and the polymorphic site in brackets:
VQGNTDEAQEECAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:34) VQGNTDEAQEEKAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:35) VQGNTDEAQEELHWKIAKMIVSD[I/V]MQQ; (SEO ID N0:36) VQGNTDEAQEELKWKIAKMIVSD[I/V]MQO.; (SEQ ID N0:37) VQGNTDEAQEELMWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:38) VQGNTDEAQEELNWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:39) ~ VQGNTDEAQEELVWKIAKMIVSD[I/V]MQQ;(SEQ ID N0:40) VQGNTDEAQEELWWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:41 ) VQGNTDEAQEELYWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:42) and VQGNTDEAQEELAWKIAKMIVSC[I/V]MQQ. (SEQ ID N0:43) C) Peptides with enhanced binding to both PKA-Rla-GFP and PKA
Rlla-GFP Substituted L-amino acids are indicated in bold, and the polymorphic site in brackets:
VQGNTDEAFEELAWKIAKMIVSD[I/V]MO.Q; (SEQ ID N0:44) VOGNTDEAIEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:45) VQGNTDEALEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:46) VQGNTDEAVEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:47) VQGNTDEAQEELAWKIAKMIVSD[I/V]FQQ; (SEQ ID N0:48) VQGNTDEAQEELAWKIAKMIVSD[I/V]IQQ; (SEQ ID N0:49) and VQGNTDEAQEELAWKIAKMIVSD[I/V]LQQ. (SEQ ID N0:50) D. Peptides with enhanced binding to PKA-Rla-GFP but normal or reduced binding to PKA-Rlla-GFP. Substituted L-amino acids are indicated in bold and the polymorphic site in brackets:
VQGNTDEAQEEFAWKIAKMIVSD[IlV]MQQ; (SEQ ID N0:51 ) VQGNTDEAQEELAWKIAKMIISD[I/V]MQQ; (SEQ ID N0:52) and VQGNTDEAQEELAWKIAKMILSD[1/V]MQQ. (SEQ ID N0:53) E. Peptide with binding to PKA-Rla-GFP but substantially no binding to PKA-Rlla-GFP Substituted L-amino acids are indicated in bold, and the polymorphic site in brackets:
VQGNTDEAQEELAWKIAKMIWSD[I/V]MQQ (SEQ ID N0:54) F. Peptides with enhanced binding to PKA-Rla-GFP but reduced binding to PKA-Rlla-GFP Substituted D-amino acids are indicated in bold and underlined and the polymorphic site in brackets:
VQGNTTEAQEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:17) VQGNTDEAFEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:18) VQGNTDEAIEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:19) VQGNTDEALEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:20) VQGNTDEAVEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:21 ) VQGNTDEAWEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:22) VQGNTDEAYEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID N0:23) VQGNTDEAQEELAWKIAKMILSD[I/V]MQQ; (SEQ ID N0:24) VQGNTDEAQEELAWKIAKMIVLD[I/V]MQQ; (SEQ, ID N0:25) and VQGNTDEAQEELAWKIAKMIVSD[I/V]FQQ. (SEQ ID N0:26) /n vivo assays of the association of AKB variants and the PKA Regulatory subunits and targeting to mitochondria.

In addition to defining the helical motif critical for binding, the peptide array revealed several key substitutions along the helix that either enhanced binding to Rla relative to Rlla or disrupted binding to Rla while maintainirig Rlla binding (see Examples 7 and 8). For example, valine at position 21, when substituted with a tryptophan abolished binding to Rlla but maintained affinity for Rla. In contrast, a single substitution at position 13, abolished binding to only the Rla isoform. Several other substitutions at positions 9, 12, 21 and seemed to enhance binding to Rla, while reducing binding to Rlla.
Using these isoform-selective positions as guides, several peptides were synthesized with the desired selectivity by using single or multiple substitutions and their activity tested in a quantitative fluorescence binding assay (Figure 2).
a) Peptide Synthesis and Fluorescence Labeling The wild type D-AKAP2 peptide referred to herein as "AKB(dual)"
(VQGNTDEAQEELAWKIAKMIVSDVMQQ; SEQ ID N0:2) for the in-solution peptide binding assays was synthesized by SynPep (Dublin, CA). The following peptides were synthesized by Anaspec (San Jose, CA):
VQGNTDEAQEELLWKIAKMIVSDVMQQ (SEQ ID N0:29; "AKB(RII)") VQGNTDEAQEELAWKIEKMIWSDVMQQ (SEQ ID N0:100; "AKB(null)") VQGNTDEAQEELAWKIAKMIWSDVMQQ (SEQ ID N0:101 ) Ac-DLAWKIAKMIVSDVMQQ (SEQ ID N0:102) Additional multiple substitution peptides (PV-37, 38, 47, 48, 49) were synthesized by Peptron (Korea). All peptides contained a C-terminal Cys for conjugation of the fluorescence probe and contained an amide protected C-terminus. The peptides were HPLC purified and the molecular mass verified by mass spectrometry. Peptide purities were greater than 95%.
Each peptide was fluorescently labeled using a 25 mM solution of tetramethylrhodamine-5-maleimide (catalog number T-6027; Molecular Probes, Eugene, OR) dissolved in DMSO. The peptides were labeled by incubating with a three-fold molar excess of the label for 16 hr at 4°C in 20 mM Tris, pH 7.0 and 1 mM tris-(2-carboxyethyl) phosphine, hydrochloride (TCEP) (non-thiol reducing agent, Molecular Probes). The sample was quenched with 1 mM /3-mercaptoethanol to bind to any unreacted maleimide and diluted with 0.1 % TFA

for purification by HPLC. The labeled peptides were resolved using a C18 column with a water/acetonitrile gradient containing 0.1 % TFA. The concentration of each peptide was determined by absorbance at 541 nm after diluting into 100% methanol and using an extinction coefficient of 91,000 M-'cm-' for absorbance of the rhodamine label at 541 nm (Molecular Probes Catalog). The peptides were stored at 4°C in 50% acetonitrile.
b) Rla and Rlla Production Full-length murine Rlla was expressed in E. coli BL21 (DE3). Full-length bovine Rla was expressed in E. coli 222. The proteins were purified as previously described using a cAMP affinity resin (Hamuro et al. (2002) J. Mo/.
Biol., 321:703). The protein concentrations were determined using the following extinction coefficients at 280 nm, which were calculated using a standard concentration of protein calibrated using quantitative amino acid analysis:
Rla 52,603 M-'cm-' and Rlla 62,456 M-'cm-'. The proteins were stored at 4°C
in 50 mM MES pH 5.8, 50 mM NaCI, 2 mM EDTA, 2 mM EGTA, 2 mM DTT.
c) Fluorescence Anisotropy Binding of each fluorescently labeled peptide to the regulatory subunits was monitored using fluorescence anisotropy. Rla and Rlla were serially diluted beginning at 1,uM and 0.1NM, respectively, into 10 mM HEPES pH 7.4, 0.15 M
NaCI, 3 mM EDTA, 0.005% Surfactant P20 (Biacore) containing either 10 nM or 1 nM of fluorescently labeled peptide for Rla and Rlla, respectively. The samples were equilibrated for at least 1 hour at room temperature and fluorescence anisotropy monitored using a Fluoromax-2 (Jobin Yvon Horiba, SPEX Division, Edison, NJ) equipped with Glan-Thompson polarizers. The rhodamine-labeled peptide was excited at 541 nm (5-10 nm bandpass) and emission monitored at 575 nm (5-10 nm bandpass). The anisotropy was calculated directly with the Fluoromax software using the following equation:
(1 ) r = (Ivv- G'~IvH)/ (Ivv + 2G~'IvH) where r is the steady state anisotropy, Ivv is the fluorescence intensity with the excitation and emission polarizers oriented in the vertical position (0 ° from normal), IvH is the fluorescence intensity with the excitation polarizes in the vertical position and the emission polarizes oriented in the horizontal position (90°) relative to the excitation polarizes, G is the monochromator grating factor which is equal to (IH~~IHH), with the first subscript indicating the position of the excitation polarizes and the second subscript indicating position of the emission polarizes. Three separate binding experiments were averaged and fit to a 1 :1 binding model using the non-linear regression application in GraphPad Prism version 3.00 (GraphPad Software, San Diego, CA).

Dissociation constants (Kp) with standard error (n = 3) for peptides binding to Rla and Rlla determined using a fluorescence anisotropy binding assay.
Rla (nM) Rlla (nM) Peptid a ( Ko) ( Kp) _AKB (dual) WT (SEQ ID N0:108) VQGNTDEAQEELAWKIAKMIVSDVMQQC 48 ~ 4 2.2 ~ 0.2 AKB (RII) RII specific (SEQ ID N0:109) VQGNTDEAQEELLWKIAKMIVSDVMQQC 2493 ~ 409 2.7 ~ 0.1 AKB (RI) RI specific (SEQ ID N0:103) FEELAWKIAKMIWSDVFQQC 5.2 ~ 0.5 456 ~ 33 AKB (null) (SEQ ID N0:110) VQGNTDEAQEELAWKIEKMIWSDVMQQC 998 ~ 66 > 10,000 Substituted residues are underlined and in bold.
The binding affinities of three peptides designated Rlla-specific (A13L;
SEQ ID N0:109), Rla-specific (0.9F, V21 W, and M25F; SEQ ID N0:103) and null (A17E and V21 W; SEQ ID N0:1 10) were compared with the unsubstituted peptide (Figure 2 and Table 7) using fluorescence anisotropy. The results shown in Table 7 and Figure 2 indicate that the A13L substitution did not affect binding to Rlla, but dramatically affected binding to Rla, establishing this peptide as an Rlla-specific peptide, AKB(RII) (Table 7). The triple substituted Rla-specific peptide not only resulted in a considerable reduction in affinity to Rlla, but also enhanced binding to Rla by a factor of ten (Table 7). The null peptide bound very poorly to Rla and binding to Rlla could not be detected (Figure 2).
c) Assay of truncated and substituted peptides Several additional truncated and substituted peptides were synthesized and their affinity to Rla and Rlla evaluated using fluorescence anisotropy (see Table 8). Binding was evaluated for both Rla and Rlla using fluorescence anisotropy as described herein. Substituted residues are in bold Table 8.
Tryptophan at a position corresponding to residue 21 of SEQ ID N0:2 is important for discriminating against binding to the Rlla subunit. PV-38 (SEQ
ID
N0:103) is designated an RI-specific binding peptide because it exhibits enhanced binding to Rla and substantially no binding (e.g., very weak binding) to Rlla compared to unmodified SEQ ID N0:108, AKB (RI), and is indicated with an asterisk.

Kp (nM) Rla RIla SEQ ID N0:108 . . . QEELAWKIAKMIVSDVMOQC48 4 2.2 0.2 SEQ ID N0:111 . . . QEELAWKIAKMIWSDVMQQC120 13 83 7 SEQ ID N0:112 Ac-DLAWKIAKMIVSDVMQQC 773 49 107 5 PV-37 FEELAWKIAKMIWSDVMQQC 19 0.7 150 11 PV-38 FEELAWKIAKMIWSDVFQQC 5.2 0.5 456 33 PV-49 FEELAWKIAKMIISDVFQQC 1.1 0.1 2.5 0.2 PV-37 (SEQ ID N0:104); PV-38 (SEQ ID
N0:103); PV-47 (SEQ ID N0:105); PV-48 (SEQ ID

N0:106); PV-49 (SEQ ID N0:107) To determine whether the N-terminal negative charges were important for high affinity binding as suggested from the truncation data, a truncation peptide corresponding to SEQ ID N0:102 was synthesized that did not contain the two N-terminal Glu residues, but instead contained an N-terminal a-acetylated Asp.
This peptide also showed reduced binding to both regulatory subunits confirming the requirement of at least one of the N-terminal negative charges. Also evident from these mutations is the importance of the bulky hydrophobic tryptophan at position 21 in selectively reducing the affinity to the type II isoform. The single mutant V21 W (SEQ ID N0:1 1 1 showed dramatically reduced binding to Rlla, while only showing modestly decreased affinity toward Rla (Table 8). This position is therefore a critical position along the helix for establishing RI/RII
selectivity. In the background of V21 W, further substitutions at position 9 and 25 dramatically enhanced binding to Rla while further disrupting Rlla binding (see PV-37 and PV-38, Table 8). Rlla also seemed to be less tolerant of the Leu to Phe substitution at position 12 (PV-47 and PV-48, Table 8). PV-49, which is identical to PV-38 except that it has an Ile instead of a Trp at position 21, bound with the greatest affinity to Rla (Table 8). However, this peptide also bound very tightly to Rlla. This again reinforced that a bulky aromatic residue at position 21 was important to select against Rlla binding. Interestingly, the Phe at positions 9 and 25 are only disruptive to Rlla binding when Trp is present at position 21 (PV-38). When Trp at position 21 is replaced with Ile, the affinity for Rlla is restored and the affinity for Rla further enhanced (PV-49). Thus, it is contemplated herein that Trp at position 21 (i) and the Phe at position 25 (i + 4) may interact to form additional unfavorable interactions for Rlla binding.

/n vivo assays of the association of AKB variants and the PKA Regulatory subunits and targeting to mitochondria.
To test the ability of the mutations set forth in Table 7 [AKB(dual), AKB(RI), AKB(RII) and AKB(null)] to co-localize with selected PKA isoforms in cells, a flag-tagged AKB domain construct targeted to the mitochondria was prepared using the AKB domain and a mitochondria targeting sequence from D-AKAP1 as set forth in Example 3. The respective mutations were then incorporated into the AKB domain of D-AKAP2 to test for selectivity in the cell.
The targeting constructs of the AKB domain were made by fusing the C-terminal 156 residues of mouse D-AKAP2 with the N-terminal mitochondrial-targeting domain of D-AKAP1 (residues 1-30 of D-AKAP1a). After adding a flag tag to the C-terminus, the constructs were sub-cloned into the pCl vector (Promega, Madison, WI). The mutations of the AKB domain corresponding to those set forth in Table 7 herein were made with the QuickChange mutagenesis method (Makarova et al. (2000) Biotechnigues, 29:970-972). Full-length bovine Rla and mouse Rlla were fused with green fluorescent protein (GFP) by subcloning into pEGFPN1 (Clontech, Palo Alto, CA). Equal amounts of the targeting constructs and either GFP-Rla or GFP-Rlla constructs were co-transfected into 1 OT(1 /2) cells using Lipofectamin (Invitrogen, Carlsbad, CA). The AKB domain was detected by immuno-staining with monoclonal antibodies against the Flag-tag (Kodak, Rochester, NY) followed by a rhodamine-conjugated secondary antibody (Jackson Lab, Bar Harbor, ME). The cells were imaged using a radiance confocal microscope (BioRad, Hercules, CA).
By concentrating D-AKAP2 at the mitochondria, the co-localization of the AKB domain and the R isoforms was easily detected. GFP constructs of Rla and Rlla were co-transfected into 10T (1/2) cells with Flag-tagged wild type AKB(dual), AKB(RII), AKB(RI) and AKB(null). The results indicated that all of the AKB domains localized well to the mitochondria. The AKB(dual) was able to recruit both GFP-Rla and GFP-Rlla to the mitochondria. The RII-specific peptide, AKB(RII), recruited RII to the mitochondria but was incapable of recruiting GFP-RI. In contrast, the RI-specific peptide, AKB(RI), only localized GFP-RI to the mitochondria; GFP-RII was not localized by the targeted AKB(RI) peptide. The null peptide, AKB(null), could not co-localize either GFP-Rla or Rlla.

Construction of transgenic knock-in mice containing single specific amino acid substitutions in D-AKAP2.
Constructs to knock-in specific amino acid substitutions in mice are designed as follows, which results in a change in the binding of D-AKAP2 to both PKA isoforms (containing RI or RII subunits) and subsequently the D-AKAP2 mediated function. The mouse D-AKAP2 cDNA sequence has been cloned (see, Huang et al. (1997) PNAS, 94:1 1 184-1 1 189) and is available at accession #
NM 019921 . Although Huang et al., believed the 372 amino acid protein was full-length, it may represent a truncated version of the mouse D-AKAP2 protein.
The genomic organization and structure of the mouse D-AKAP2 gene is available in draft form from Genbank (accession #AL646042 and #AC084019). The _97_ mouse protein and nucleic acid sequences are set forth in SEQ ID NOs:55 and 56. In SEQ ID NOs:55 and 56, the first amino acid (met) corresponds to amino acid position 291 in the human D-AKAP2 protein set forth as SEQ ID NOs:64 and 65. The PKA binding sequence (27-amino acid peptide) corresponds to amino acids 333-359 of the mouse D-AKAP2 protein SEO ID N0:56 and to amino acids 623-649 of the human D-AKAP2 protein SEQ ID NOs:64 and 65.
A) Based on the mouse cDNA sequence set forth in SEQ ID N0:55, a modified D-AKAP2 knock-in mouse is created, using well-known methods, by introducing a Q91 substitution in the region of mouse D-AKAP2 corresponding to the 27-mer peptide region set forth in SEQ ID NOs:1 and 2 (which corresponds to a Q341 I substitution in the mouse D-AKAP2 protein set forth in SEQ ID
N0:56). The knock-in is created by replacing the codon corresponding to nucleotides 1021-1023 of SEQ ID N0:55 with either one of the codons "ATT"
or "ATC". The phenotype of this particular Q3411 knock-in mouse corresponds to enhanced binding of the modified D-AKAP2 protein to PKA-Rla-GFP, but normal binding to PKA-Rlla-GFP.
B) Based on the mouse cDNA sequence set forth in SEQ ID N0:55, a modified D-AKAP2 knock-in mouse is created, using well-known methods, by introducing an A13L substitution in the region of mouse D-AKAP2 corresponding to the 27-mer peptide region set forth in SEQ ID NOs:1 and 2 (which corresponds to a A345L substitution in the mouse D-AKAP2 protein set forth in SEQ ID N0:56). The knock-in is created by replacing the codon corresponding to nucleotides 1033-1035 of SEQ ID N0:55 with either one of the codons "TTA", "TTG", "CTT", "CTC", "CTA" or "CTG". The phenotype of this particular A345L knock-in mouse corresponds to disrupted binding of the modified D-AKAP2 protein to PKA-Rla-GFP, but normal binding to PKA-Rlla-GF.
C) Based on the mouse cDNA sequence set forth in SEQ ID N0:55, a modified D-AKAP2 knock-in mouse is created, using well-known methods, by introducing a V21 W substitution in the region of mouse D-AKAP2 corresponding to the 27-mer peptide region set forth in SEQ ID NOs:1 and 2 (which corresponds to a V353W substitution in the mouse D-AKAP2 protein set forth in SEQ ID N0:56). The knock-in is created by replacing the codon corresponding _98_ to nucleotides 1057-1059 of SEQ ID N0:55 with either one of the codons "TGA" or "TGG". The phenotype of this particular V353W knock-in mouse corresponds to normal binding of the modified D-AKAP2 protein to PKA-Rla-GFP, but disrupted binding to PKA-Rlla-GF.

Construction of transgenic triple-mutant knock-in mice containing three specific amino acid substitutions in D-AKAP2.
Based on the mouse cDNA sequence set forth in SEQ ID N0:55, a modified D-AKAP2 knock-in mouse is created, using well-known methods, by introducing a Q9F, V21 W, M25F triple-mutant substitution in the region of mouse D-AKAP2 corresponding to the 27-mer peptide region set forth in SEQ ID
NOs:1 and 2 (which corresponds to a Q341 F, V353W, M357F substitution in the mouse D-AKAP2 protein set forth in SEQ ID N0:56). The knock-in is created by replacing the codon corresponding to nucleotides 1021-1023 (9QF) of SEQ
ID N0:55 with either one of the codons "TTT" or "TTC"; by replacing the codon corresponding to nucleotides 1057-1059 of SEQ ID N0:55 with either one of the codons "TGA" or "TGG"; and replacing the codon corresponding to nucleotides 1069-1071 of SEQ ID N0:55 with either one of the codons "TTT" or "TTC".
The phenotype of this particular Q341 F, V353W, M357F triple-mutant knock-in mouse corresponds to Rla-specific binding (e.g., corresponding to approximately 10-fold increased binding to Rla and approximately 220-fold decreased binding affinity for Rlla; see Table 7).
Since modifications will be apparent to those of skill in this art, it is intended that this invention be limited only by the scope of the appended claims.

SEQUENCE LISTING
<110> SEQUENOM, INC.
Braun, Andreas Cantor, Charles Kammerer, Stefan Taylor, Susan Hamuro, Lora Cook, Charles Olson, Gary Self, Christopher <120> KINASE ANCHOR PROTEIN MUTEINS, PEPTIDES THEREOF, AND RELATED METHODS
<130> 24736-2065PC
<140> Not Yet Assigned <141> Herewith <150> 60/377,852 <151> 2002-05-03 <150> 60/453,408 <151> 2003-03-07 <160> 112 <170> FastSEQ for Windows Version 4.0 <210> 1 <211> 27 <212> PRT
<213> Homo Sapiens <400> 1 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 2 <211> 27 <212> PRT
<213> Homo Sapiens <400> 2 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Val Met Gln Gln <210> 3 <211> 21 <212> PRT
<213> Homo Sapiens <400> 3 Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile <210> 4 <211> 15 <212> PRT
<213> Homo Sapiens <400> 4 Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val <210> 5 <211> 25 <212> PRT
<213> Homo Sapiens <400> 5 Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln <210> 6 <211> 23 <212> PRT
<213> Homo Sapiens <400> 6 Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met <210> 7 <211> 21 <212> PRT
<213> Homo Sapiens <400> 7 Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile <210> 8 <211> 19 <212> PRT
<213> Homo Sapiens <400> 8 Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp <210> 9 <211> 17 <212> PRT
<213> Homo Sapiens <400> 9 Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser <210> 10 <211> 13 <212> PRT
<213> Homo Sapiens <400> 10 Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile <210> 11 <211> 11 <212> PRT
<213> Homo Sapiens <400> 11 Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met <210> 12 <211> 9 <212> PRT
<213> Homo Sapiens <400> 12 Glu Glu Leu Ala Trp Lys Ile Ala Lys <210> 13 <211> 7 <212> PRT
<213> Homo Sapiens <400> 13 Glu Leu Ala Trp Lys Ile Ala <210> 14 <211> 30 <212> PRT
<213> Homo Sapiens <220>
<221> MOD_RES
<222> 1 <223> Xaa is biotin <221> MOD_RES
<222> 2,3 <223> bAla <400> 14 Xaa Xaa Xaa Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 15 <211> 28 <212> PRT

<213> Homo Sapiens <220>
<221> ACETYLATION
<222> 28 <223> Xaa is c-terminal acetylation <400> 15 Gln Gln Met Val Asp Ser Val Ile Met Lys Ala Ile Lys Trp Ala Leu Glu Glu Gln Ala Glu Asp Thr Asn Gly Gln Val Xaa <210> 16 <211> 28 <212> PRT
<213> Homo Sapiens <220>
<221> ACETYLATION
<222> 28 <223> Xaa is C-terminal acetylation <400> 16 Gln Gln Met Ile Asp Ser Val Ile Met Lys Ala Ile Lys Trp Ala Leu Glu Glu Gln Ala Glu Asp Thr Asn Gly Gln Val Xaa <210> 17 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> MOD_RES
<222> 6 <223> Xaa is D-Threonine <221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 17 Val Gln Gly Asn Thr Xaa Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Xaa Met Gln Gln <210> 18 <211> 26 <212> PRT
<213> Homo Sapiens <220>
<221> MOD_RES
<222> 9 <223> Xaa is D-Phenylalanine <221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 18 Val Gln Gly Asn Thr Asp Glu Ala Xaa Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Xaa Met Gln Gln <210> 19 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> MOD_RES
<222> 9 <223> Xaa is D-Ile <221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 19 Val Gln Gly Asn Thr Asp Glu Ala Xaa Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Xaa Met Gln Gln <210> 20 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> MOD_RES
<222> 9 <223> Xaa is D-Leucine <221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 20 Val Gln Gly Asn Thr Asp Glu Ala Xaa Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Xaa Met Gln Gln <210> 21 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> MOD_RES
<222> 9 <223> Xaa is D-Valine <221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 21 Val Gln Gly Asn Thr Asp Glu Ala Xaa Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Xaa Met Gln Gln <210> 22 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> MOD_RES
<222> 9 <223> Xaa is D-Tryptophan <221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 22 Val Gln Gly Asn Thr Asp Glu Ala Xaa Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Xaa Met Gln Gln <210> 23 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> MOD_RES
<222> 9 <223> Xaa is D-Tyrosine <221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 23 Val Gln Gly Asn Thr Asp Glu Ala Xaa Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Xaa Met Gln Gln <210> 24 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> MOD_RES
<222> 21 <223> Xaa is D-Leucine <221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 24 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Xaa Ser Asp Xaa Met Gln Gln <210> 25 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> MOD_RES
<222> 21 <223> Xaa is D-Leucine <221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 25 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Xaa Asp Xaa Met Gln Gln <210> 26 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> VARIANT
<222> 24 <223> Xaa is Ile or Val <221> MOD_RES
<222> 25 <223> Xaa is D-Phenylalanine <400> 26 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Xaa Xaa Gln Gln <210> 27 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 27 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Phe Trp Lys Ile Ala Lys Met Ile Val Ser Asp Xaa Met Gln Gln <210> 28 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> VARIANT

_g_ <222> 24 <223> Xaa is Ile or Val <400> 28 Val Gln Gly Asn Thr Asp GlnGlu Glu Ile Trp Lys Glu Ala Leu Ile Ala Lys Met Ile Val Ser MetGln Gln Asp Xaa <210> 29 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 29 Val Gln Gly Asn Thr Asp GlnGlu Glu Leu Trp Lys Glu Ala Leu Ile Ala Lys Met Ile Val Ser MetGln Gln Asp Xaa <210> 30 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 30 Val Gln Gly Asn Thr Asp GlnGlu Glu Ala Trp Cys Glu Ala Leu Ile Ala Lys Met Ile Val Ser MetGln Gln Asp Xaa <210> 31 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 31 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Cys Met Ile Val Ser Asp Xaa Met Gln Gln <210> 32 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 32 Val Gln Gly Asn Gln Glu Thr Asp Glu Ala Glu Leu Ala Trp Lys Ile Ala Lys Cys Ile Met Gln Val Ser Asp Xaa Gln <210> 33 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile Val or <400> 33 Val Gln Gly Asn Asp Glu Gln Glu Leu Ala Trp Lys Thr Ala Glu Ile Ala Lys Met Ile Cys Asp Met Gln Val Xaa Gln <210> 34 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile Val or <400> 34 Val Gln Gly Asn Asp Glu Gln Glu Cys Ala Trp Lys Thr Ala Glu Ile Ala Lys Met Ile Ser Asp Met Gln Val Xaa Gln <210> 35 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile Val or <400> 35 Val Gln Gly Asn Asp Glu Lys Ala Trp Lys Thr Ala Gln Ile Glu Glu Ala Lys Met Ile Ser Asp Val Xaa Met Gln Gln <210> 36 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 36 Val Gln Gly Asn Thr Asp Glu Glu Leu Trp Lys Glu Ala Gln His Ile Ala Lys Met Ile Val Ser Gln Gln Asp Xaa Met <210> 37 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 37 Val Gln Gly Asn Thr Asp Ala Glu Glu Leu Trp Lys Glu Gln Lys Ile Ala Lys Met Ile Val Ser Xaa Gln Gln Asp Met <210> 38 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 38 Val Gln Gly Asn Thr Asp Ala Glu Glu Leu Trp Lys Glu Gln Met Ile Ala Lys Met Ile Val Ser Xaa Gln Gln Asp Met 2p 25 <210> 39 <211> 27 <212> PRT

<213> Homo sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 39 Val Gln Gly Asn Thr Asp Ala Glu Gln Glu Glu Leu Asn Trp Lys Ile Ala Lys Met Ile Val Ser Xaa Asp Met Gln Gln <210> 40 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 40 Val Gln Gly Asn Thr Asp Glu Glu Leu Trp Lys Glu Ala Gln Val Ile Ala Lys Met Ile Val Ser Gln Gln Asp Xaa Met <210> 41 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 41 Val Gln Gly Asn Thr Asp Ala Glu Glu Leu Trp Lys Glu Gln Trp Ile Ala Lys Met Ile Val Ser Xaa Gln Gln Asp Met <210> 42 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 42 Val Gln Gly Asn Thr Asp Ala Glu Glu Leu Trp Lys Glu Gln Tyr Ile Ala Lys Met Ile Val Ser Xaa Gln Gln Asp Met <210> 43 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 43 Val Gln Gly Asn Thr Asp Ala Glu Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Xaa Cys Met Gln Gln <210> 44 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 44 Val Gln Gly Asn Thr Asp Glu Glu Leu Trp Lys Glu Ala Phe Ala Ile Ala Lys Met Ile Val Ser Gln Gln Asp Xaa Met <210> 45 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 45 Val Gln Gly Asn Thr Asp Ala Glu Glu Leu Trp Lys Glu Ile Ala Ile Ala Lys Met Ile Val Ser Xaa Gln Gln Asp Met 2p 25 <210> 46 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 46 Val Gln Gly Asn Thr Asp Ala Glu Glu Leu Trp Lys Glu Leu Ala Ile Ala Lys Met Ile Val Ser Xaa Gln Gln Asp Met <210> 47 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 47 Val Gln Gly Asn Thr Asp Ala Glu Val Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Xaa Asp Met Gln Gln <210> 48 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 48 Val Gln Gly Asn Thr Asp Glu Glu Glu Ala Gln Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Gln Gln Asp Xaa Phe <210> 49 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 49 Val Gln Gly Asn Thr Asp Ala Glu Glu Ala Trp Lys Glu Gln Leu Ile Ala Lys Met Ile Val Ser Xaa Gln Gln Asp Ile <210> 50 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 50 Val Gln Gly Asn Thr Asp Ala Glu Glu Ala Trp Lys Glu Gln Leu Ile Ala Lys Met Ile Val Ser Xaa Gln Gln Asp Leu <210> 51 <211> 27 <212> PRT

<213> Homo Sapiens <220>

<221> VARIANT

<222> 24 <223> Xaa is Ile or Val <400> 51 Val Gln Gly Asn Thr Asp Ala Ala Trp Lys Glu Gln Ile Glu Glu Phe Ala Lys Met Ile Val Ser Xaa Asp Met Gln Gln <210> 52 <211> 27 <212> PRT

<213> Homo Sapiens <220>
<221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 52 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Ile Ser Asp Xaa Met Gln Gln <210> 53 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 53 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Leu Ser Asp Xaa Met Gln Gln <210> 54 <211> 27 <212> PRT
<213> Homo Sapiens <220>
<221> VARIANT
<222> 24 <223> Xaa is Ile or Val <400> 54 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Trp Ser Asp Xaa Met Gln Gln <210> 55 <211> 1119 <212> DNA
<213> Mus musculus <220>
<221> CDS
<222> (1)...(1119) <223> cDNA: truncated version of mouse D-AKAP2 <400> 55 atg aaa agt ata gaa caa gat gca gtg aat act ttt acc aaa tat ata 48 Met Lys Ser Ile Glu Gln Asp Ala Val Asn Thr Phe Thr Lys Tyr Ile tct cca gat get get aag cca ata cca att aca gaa gcc atg aga aac 96 Ser Pro Asp Ala Ala Lys Pro Ile Pro Ile Thr Glu Ala Met Arg Asn gac atc atc gca aag att tgt gga gaa gat gga cag gtg gat ccc aac 144 Asp Ile Ile Ala Lys Ile Cys Gly Glu Asp Gly Gln Val Asp Pro Asn tgt ttt caa 192 ttc agt gag gtt gca ctg atg gac gag acg get gta gtc Cys Phe Gln Phe Ser Glu Val Ala Leu Met Asp Glu Thr Ala Val Val cac agt ctg cac catttctgt taccag 240 ttt gag cga aaa att ttt agt His Ser Leu His HisPheCys TyrGln Phe Glu Arg Lys Ile Phe Ser aa ctg agtggg act tac ctggetgat atcctcttc tgt 288 gtg acc gtt g Leu SerGly Thr Tyr LeuAlaAsp IleLeuPhe Cys Glu Thr Val Val a tcagcc ctcttttat ttttctgag tacatggaa aaagaagat gca 336 g SerAla LeuPheTyr PheSerGlu TyrMetGlu LysGluAsp Ala g Glu gtg aatatc ttacaattc tggttagca gcggataat ttccagtct cag 384 Val AsnIle LeuGlnPhe TrpLeuAla AlaAspAsn PheGlnSer Gln ctt getgcc aaaaagggc cagtatgat ggacaggag gcccagaat gat 432 Leu AlaAla LysLysGly GlnTyrAsp GlyGlnGlu AlaGlnAsn Asp gcc atgatt ttatatgac aagtacttt tccctccaa gccacacac ccc 480 Ala MetIle LeuTyrAsp LysTyrPhe SerLeuGln AlaThrHis Pro ctt ggattt gatgatgtt gtacgatta gaaattgaa tctaatatc tgc 528 Leu GlyPhe AspAspVal ValArgLeu GluIleGlu SerAsnIle Cys a gaaggt ggaccactt cctaattgt ttcacaact ccattacgt cag 576 gg GluGly GlyProLeu ProAsnCys PheThrThr ProLeuArg Gln Arg cc tggaca accatggag aaggtcttt ttgcctggt tttctgtcc agc 624 g TrpThr ThrMetGlu LysValPhe LeuProGly PheLeuSer Ser Ala aat ctttat tacaaa~tatttgaatgat ctcatccat tcagttcga gga 672 Asn LeuTyr TyrLysTyr LeuAsnAsp LeuIleHis SerValArg Gly gat gaa cttgga aatgtttcc getget cacggctct gtc 720 ttt ggg ctg Asp Glu LeuGly AsnValSer AlaAla HisGly Val Phe Gly Leu Ser tgc ctt gaggag cactca tccgat ggctcc get 768 cct tct ggt act ggt Cys Leu Glu Ser Gly Ala Pro Glu Gly Ser Ser Gly Thr His Ser Asp cag tct agt gtg aaa aaa gcc agt att aaa att ctg aaa aat ttt gat 816 Gln Ser Ser Val Lys Lys Ala Ser Ile Lys Ile Leu Lys Asn Phe Asp gaa gca ata att gtg gat get gca agt ctg gac cca gaa tct tta tat 864 Glu Ala Ile Ile Val Asp Ala Ala Ser Leu Asp Pro Glu Ser Leu Tyr caacgg acatatgca gggaagatg tcctttggg agagtt agtgatttg 912 GlnArg ThrTyrAla GlyLysMet SerPheGly ArgVal SerAspLeu gggcag ttcatccga gagtctgag cctgaacct gatgtg aagaaatca 960 GlyGln PheIleArg GluSerGlu ProGluPro AspVal LysLysSer aa a ttcatgttc tcacaaget atgaagaag tgggtg caaggaaat 1008 a gg PheMetPhe SerGlnAla MetLysLys TrpVal GlnGlyAsn LysGly actgac gaggcccaa gaagagcta gettggaag attgca aaaatgata 1056 ThrAsp GluAlaGln GluGluLeu AlaTrpLys IleAla LysMetIle gtgagt gatgttatg cagcaggca caccatgat caacca ctagagaag 1104 ValSer AspValMet GlnGlnAla HisHisAsp GlnPro LeuGluLys tctaca aagctatga SerThr LysLeu <210> 56 <211> 372 <212> PRT
<213> Mus musculus <400> 56 Met Lys Ser Ile Glu Gln Asp Ala Val Asn Thr Phe Thr Lys Tyr Ile Ser Pro Asp Ala Ala Lys Pro Ile Pro Ile Thr Glu Ala Met Arg Asn Asp Ile Ile Ala Lys Ile Cys Gly Glu Asp Gly Gln Val Asp Pro Asn Cys Phe Val Leu Asp Thr Ala Val Val Phe Ser Ala Met Glu Gln Glu His Phe Ser Glu Phe Leu Arg Ser His His Phe Cys Lys Tyr Gln Ile Glu Val Leu Thr Ser Gly Thr Val Tyr Leu Ala Asp Ile Leu Phe Cys Glu Ser Ala Leu Phe Tyr Phe Ser Glu Tyr Met Glu Lys Glu Asp Ala Val Asn Ile Leu Gln Phe Trp Leu Ala Ala Asp Asn Phe Gln Ser Gln Leu Ala Ala Lys Lys Gly Gln Tyr Asp Gly Gln Glu Ala Gln Asn Asp Ala Met Ile Leu Tyr Asp Lys Tyr Phe Ser Leu Gln Ala Thr His Pro Leu Gly Phe Asp Asp Val Val Arg Leu Glu Ile Glu Ser Asn Ile Cys Arg Glu Gly Gly Pro Leu Pro Asn Cys Phe Thr Thr Pro Leu Arg Gln Ala Trp Thr Thr Met Glu Lys Val Phe Leu Pro Gly Phe Leu Ser Ser Asn Leu Tyr Tyr Lys Tyr Leu Asn Asp Leu Ile His Ser Val Arg Gly Asp Glu Phe Leu Gly Gly Asn Val Ser Leu Ala Ala His Gly Ser Val Cys Leu Pro Glu Glu Ser His Ser Gly Gly Ser Asp Gly Ser Thr Ala Gln Ser Ser Val Lys Lys Ala Ser Ile Lys Ile Leu Lys Asn Phe Asp Glu Ala Ile Ile Val Asp Ala Ala Ser Leu Asp Pro Glu Ser Leu Tyr Gln Arg Thr Tyr Ala Gly Lys Met Ser Phe Gly Arg Val Ser Asp Leu Gly Gln Phe Ile Arg Glu Ser Glu Pro Glu Pro Asp Val Lys Lys Ser Lys Gly Phe Met Phe Ser Gln Ala Met Lys Lys Trp Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Val Met Gln Gln Ala His His Asp Gln Pro Leu Glu Lys Ser Thr Lys Leu <210> 57 <211> 159 <212> DNA
<213> Artificial Sequence <220>
<223> Construct <221> CDS
<222> (37)...(159) <223> cDNA construct <400> 57 aaaggattca tgttctcaca agctatgaag aagtgg gtg caa gga aat act gac 54 Val Gln Gly Asn Thr Asp gag gcc aty gaa gag cta get tgg aag att gca aaa atg ata gtg agt 102 Glu Ala Xaa Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser gat gtt atg cag cag gca cac cat gat caa cca cta gag aag tct aca 150 Asp Val Met Gln Gln Ala His His Asp Gln Pro Leu Glu Lys Ser Thr aag cta tga Lys Leu <210> 58 <211> 40 <212> PRT
<213> Artificial Sequence <220>
<221> VARIANT
<222> 9 <223> Xaa is Ile <400> 58 Val Gln Gly Asn Thr Asp Glu Ala Xaa Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Val Met Gln Gln Ala His His Asp Gln Pro Leu Glu Lys Ser Thr Lys Leu <210> 59 <211> 159 <212> DNA

<213> Artificial Sequence <220>

<223> Construct <221> CDS

<222> (37)...(159) <223> cDNA construct <221> mutation <222> 75 <223> n is a or g or c or t <400> 59 aaaggattca tgttctcaca aagtgg gtgcaa ggaaatact gac 54 agctatgaag ValGln GlyAsnThr Asp gag gcc caa gaa gag tgg aagattgcaaaa atgatagtg agt 102 cta ttn Glu Ala Gln Glu Glu Trp LysIleAlaLys MetIleVal Ser Leu Xaa gat gtt atg cag cag cat gatcaaccacta gagaagtct aca 150 gca cac Asp Val Met Gln Gln His AspGlnProLeu GluLysSer Thr Ala His aag cta tga Lys Leu <210> 60 <211> 40 <212> PRT
<213> Artificial Sequence <220>
<221> VARIANT
<222> 13 <223> Xaa is Leucine <400> 60 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Xaa Trp Lys Ile Ala Lys Met Ile Val Ser Asp Val Met Gln Gln Ala His His Asp Gln Pro Leu Glu Lys Ser Thr Lys Leu <210> 61 <211> 159 <212> DNA
<213> Artificial Sequence <220>
<223> Construct <221> CDS

<222> (37)...(159) <223> cDNA construct <400> 61 aaaggattca tgttctcaca agctatgaag aagtgg gtg caa gga aat act gac 54 Val Gln Gly Asn Thr Asp gag gcc caa gaa gag cta get tgg aag att gca aaa atg ata tgr agt 102 Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Xaa Ser gat gtt atg cag cag gca cac cat gat caa cca cta gag aag tct aca 150 Asp Val Met Gln Gln Ala His His Asp Gln Pro Leu Glu Lys Ser Thr aag cta tga Lys Leu <210> 62 <211> 40 <212> PRT

<213> Artificial Sequence <220>

<221> VARIANT

<222> 21 <223> Xaa is Tryptophan <400> 62 Val Gln Gly Asn Thr Asp Glu Ala Gln Leu Ala Lys Ile Glu Glu Trp Ala Lys Met Ile Xaa Ser Asp Val Met Ala His Asp Gln Gln Gln His Pro Leu Glu Lys Ser Thr Lys Leu <210> 63 <211> 2363 <212> DNA

<213> Homo Sapiens <220>

<221> CDS

<222> (138)...(2126) <223> Wild Type AI~AP-10 <300>

<308> GenBank AF037439 <309> 1997-12-21 <400> 63 gcggcttgtt gataatatgg cggctggagc tcccgaggaggcggtggggc60 tgcctgggca CCaCtCCCgg aagaagggtc ccttttcgcg ggcccctctggacccggaag120 ctagtgcagc tccgggccgg ttgctga atg agg gga gcc tcc ccg cag tcc 170 ggg CCC cgc Met Arg Gly Ala Gly Pro Ser Pro Gln Ser Arg CCC CgC aCC CtC Cgt ccc gac ccg ggc atg tcc 218 ccc gcc ttc ttc cgg Pro Arg Thr Leu Arg Pro Asp Pro Gly Met Ser Pro Ala Phe Phe Arg cgg aaa 266 gtg aaa ggc aaa gaa caa gag aag acc tca gat gtg aag tcc Arg Lys Val Lys Gly Lys Glu Gln Glu Lys Thr Ser Asp Val Lys Ser att aaa act 314 get tca aaa ata tcc aat gta cat tcc cca caa aaa agc Ile Lys Ser Ala Ser Thr Ile Ser Lys Val His Asn Ser Pro Gln Lys cat gcc gga cca gcaatc 362 ttg ctg agt cat aat gag get gtt gcc gca His Ala Gly Pro AlaIle Leu Leu Ser His Asn Glu Ala Val Ala Ala att tct ttt tca aggacagcc acactt 410 gcc aac agt agc atg gac tcc Ile Ser Phe Ser Ser ArgThrAla Leu Ala Asn Ser Thr Met Asp Ser aag aag cca agc cac gag get cat tttggtgac ctgggc 458 cag atg get Lys Lys Pro Ser His Glu Ala His PheGlyAsp LeuGly Gln Met Ala aga tct ctg gac tac act caa acc aaatcaagc ctttct 506 tgt cag gag Arg Ser Leu Asp Tyr Thr Gln Thr LysSerSer LeuSer Cys Gln Glu aag acc gaa caa gtc cac gac att gtcctccct tacttc 554 ctt ttg act Lys Thr Glu Gln Val His Asp Ile ValLeuPro TyrPhe Leu Leu Thr att caa atg gaa ctt cga atg cat ttggtgaaa ttttgg 602 ttc cgg gag Ile Gln Met Glu Leu Arg Met His LeuValLys PheTrp Phe Arg Glu 140 145 150 , 155 tta gag gaa agt ttt tca aca tgg tcgcgaata agagca 650 get cat act Leu Glu Glu Ser Phe Ser Thr Trp SerArgIle ArgAla Ala His Thr cac agt aac aca atg cag agc ctg getgagCCt gtCtct 698 cta aag tca His Ser Asn Thr Met Gln Ser Leu AlaGluPro ValSer Leu Lys Ser cca tct aag cat gaa aca gcg ttt ttaactgat tctctt 746 aaa act tct Pro Ser Lys His Glu Thr Ala Phe LeuThrAsp SerLeu Lys Thr Ser gat aag ttg gag gat ggc tca cag ttgtttatg actcat 794 aga tct gca Asp Lys Leu Glu Asp Gly Ser Gln LeuPheMet ThrHis Arg Ser Ala tca gaa att gac ctg aat aga aac agcactcag aatcac 842 gga aat act Ser Glu Ile Asp Leu Asn Arg Asn SerThrGln AsnHis Gly Asn Thr ttg ctg tcc cag gaa gac agt cat tctCtCCgt cttgaa 890 ctt tgt gcc Leu Leu Ser Gln Glu Asp Ser His SerLeu LeuGlu Leu Cys Ala Arg atg gcc gca gga act atg gaa gaatct 938 aga cac caa gtt acc tcc caa Met Ala Ala Gly Thr Gln Val Met Glu Glu Arg His Ser Thr Ser Gln tcc tct ctt aca gta agt ccc cca 986 aca gcc agt aga get cta aat tct Ser Ser Thr Leu Thr Val Ala Ser Arg Asn Ser Pro Ala Ser Pro Leu aaagaa ttgtcagga aaactaatg aaaagtata gaacaagat gcagtg 1034 LysGlu LeuSerGly LysLeuMet LysSerIle GluGlnAsp AlaVal aatact tttaccaaa tatatatct ccagatget getaaacca atacca 1082 AsnThr PheThrLys TyrIleSer ProAspAla AlaLysPro IlePro attaca gaagcaatg agaaatgac atcatagca aggatttgt ggagaa 1130 IleThr GluAlaMet ArgAsnAsp IleIleAla ArgIleCys GlyGlu gatgga caggtggat cccaactgt ttcgttttg gcacagtcc atagtc 1178 AspGly GlnValAsp ProAsnCys PheValLeu AlaGlnSer IleVal tttagt gcaatggag caagagcac tttagtgag tttctgcga agtcac 1226 PheSer AlaMetGlu GlnGluHis PheSerGlu PheLeuArg SerHis catttc tgtaaatac cagattgaa gtgctgacc agtggaact gtttac 1274.

HisPhe CysLysTyr GlnIleGlu ValLeuThr SerGlyThr ValTyr ctgget gacattctc ttctgtgag tcagccctc ttttatttc tctgag 1322 LeuAla AspIleLeu PheCysGlu SerAlaLeu PheTyrPhe SerGlu tacatg gaaaaagag gatgcagtg aatatctta caattctgg ttggca 1370 TyrMet GluLysGlu AspAlaVal AsnIleLeu GlnPheTrp LeuAla gcagat aacttccag tctcagctt getgccaaa aaggggcaa tatgat 1418 AlaAsp AsnPheGln SerGlnLeu AlaAlaLys LysGlyGln TyrAsp ggacag gaggcacag aatgatgcc atgatttta tatgacaag tacttc 1466 GlyGln GluAlaGln AsnAspAla MetIleLeu TyrAspLys TyrPhe tccctc caagccaca catcctctt ggatttgat gatgttgta cgatta 1514 SerLeu GlnAlaThr HisProLeu GlyPheAsp AspValVal ArgLeu gaaatt gaatccaat atctgcagg gaaggtggg ccactcccc aactgt 1562 GluIle GluSerAsn IleCysArg GluGlyGly ProLeuPro AsnCys ttcaca actccatta cgtcaggcc tggacaacc atggagaag gtcttt 1610 PheThr ThrProLeu ArgGlnAla TrpThrThr MetGluLys ValPhe ttgcct ggctttctg tccagcaat ctttattat aaatatttg aatgat 1658 LeuPro GlyPheLeu SerSerAsn LeuTyrTyr LysTyrLeu AsnAsp ctcatc cattcggtt cgaggagat gaatttctg ggcgggaac gtgtcg 1706 Leu HisSerVal ArgGlyAsp GluPheLeu GlyGlyAsn ValSer Ile ccgact cctggctct gttggccct cctgatgag tctcaccca ggg 1754 get ProThr ProGlySer ValGlyPro ProAspGlu SerHisPro Gly Ala agttct agctctgcg tctcagtcc agtgtgaaa aaagccagt att 1802 gac SerSer SerSerAla SerGlnSer SerValLys LysAlaSer Ile Asp aaaata aaaaatttt gatgaagcg ataattgtg gatgcggca agt 1850 ctg LysIle LysAsnPhe AspGluAla IleIleVal AspAlaAla Ser Leu ctggat gaatcttta tatcaacgg acatatgcc gggaagatg aca 1898 cca LeuAsp GluSerLeu TyrGlnArg ThrTyrAla GlyLysMet Thr Pro tttgga gtgagtgac ttggggcaa ttcatccgg gaatctgag cct 1946 aga PheGly ValSerAsp LeuGlyGln PheIleArg GluSerGlu Pro Arg gaacct gtaaggaaa tcaaaagga tccatgttc tcacaaget atg 1994 gat GluPro ValArgLys SerLysGly SerMetPhe SerGlnAla Met Asp aagaaa gtgcaagga aatactgat gaggcccag gaagagcta get 2042 tgg LysLys ValGlnGly AsnThrAsp GluAlaGln GluGluLeu Ala Trp tggaag getaaaatg atagtcagt gacattatg cagcagget cag 2090 att TrpLys AlaLysMet IleValSer AspIleMet GlnGlnAla Gln Ile tatgat ccgttagag aaatctaca aagttatga ctcaaaactt 2136 caa TyrAsp ProLeuGlu LysSerThr LysLeu Gln gagataaaggaaatctgctt aaata ttt cccttggttggattctt2196 gtgaa agagaacttt caacacagccaatgaaaa ca atatt tgatctgt cac tgttgtttccagggaga 2256 gcact tc gaatggggag acaatcct ag ccacc aatgcagt tac ctgtagggcataattgg2316 gactt ct atggcacatg atgtttcaca aggag tttaaagg ttaccaa cagtg tc <210>

<211>

<212>
PRT

<213> Sapiens Homo <400> 64 Met Arg Gly Ala Gly Pro Ser Pro Arg Gln Ser Pro Arg Thr Leu Arg Pro Asp Pro Gly Pro Ala Met Ser Phe Phe Arg Arg Lys Val Lys Gly Lys Glu Gln Glu Lys Thr Ser Asp Val Lys Ser Ile Lys Ala Ser Ile Ser Val His Ser Pro Gln Lys Ser Thr Lys Asn His Ala Leu Leu Glu Ala Ala Gly Pro Ser His Val Ala Ile Asn Ala Ile Ser Ala Asn Met Asp Ser Phe Ser Ser Ser Arg Thr Ala Thr Leu Lys Lys Gln Pro Ser His Met Glu Ala Ala His Phe Gly Asp Leu Gly Arg Ser Cys Leu Asp Tyr Gln Thr Gln Glu Thr Lys Ser Ser Leu Ser Lys Thr Leu Glu Gln Val Leu His Asp Thr Ile Val Leu Pro Tyr Phe Ile Gln Phe Met Glu Leu Arg Arg Met Glu His Leu Val Lys Phe Trp Leu Glu Ala Glu Ser Phe His Ser Thr Thr Trp Ser Arg Ile Arg Ala His Ser Leu Asn Thr Met Lys Gln Ser Ser Leu Ala Glu Pro Val Ser Pro Ser Lys Lys His Glu Thr Thr Ala Ser Phe Leu Thr Asp Ser Leu Asp Lys Arg Leu Glu Asp Ser Gly Ser Ala Gln Leu Phe Met Thr His Ser Glu Gly Ile Asp Leu Asn Asn Arg Thr Asn Ser Thr Gln Asn His Leu Leu Leu Ser Gln Glu Cys Asp Ser Ala His Ser Leu Arg Leu Glu Met Ala Arg Ala Gly Thr His Gln Val Ser Met Glu Thr Gln Glu Ser Ser Ser Thr Leu Thr Val Ala Ser Arg Asn Ser Pro Ala Ser Pro Leu Lys Glu Leu Ser Gly Lys Leu Met Lys Ser Ile Glu Gln Asp Ala Val Asn Thr Phe Thr Lys Tyr Ile Ser Pro Asp Ala Ala Lys Pro Ile Pro Ile Thr Glu Ala Met Arg Asn Asp Ile Ile Ala Arg Ile Cys Gly Glu Asp Gly Gln Val Asp Pro Asn Cys Phe Val Leu Ala Gln Ser Ile Val Phe Ser Ala Met Glu Gln Glu His Phe Ser Glu Phe Leu Arg Ser His His Phe Cys Lys Tyr Gln Ile Glu Val Leu Thr Ser Gly Thr Val Tyr Leu Ala Asp Ile Leu Phe Cys Glu Ser Ala Leu Phe Tyr Phe Ser Glu Tyr Met Glu Lys Glu Asp Ala Val Asn Ile Leu Gln Phe Trp Leu Ala Ala Asp Asn Phe Gln Ser Gln Leu Ala Ala Lys Lys Gly Gln Tyr Asp Gly Gln Glu Ala Gln Asn Asp Ala Met Ile Leu Tyr Asp Lys Tyr Phe Ser Leu Gln Ala Thr His Pro Leu Gly Phe Asp Asp Val Val Arg Leu Glu Ile Glu Ser Asn Ile Cys Arg Glu Gly Gly Pro Leu Pro Asn Cys Phe Thr Thr Pro Leu Arg Gln Ala Trp Thr Thr Met Glu Lys Val Phe Leu Pro Gly Phe Leu Ser Ser Asn Leu Tyr Tyr Lys Tyr Leu Asn Asp Leu Ile His Ser Val Arg Gly Asp Glu Phe Leu Gly Gly Asn Val Ser Pro Thr Ala Pro Gly Ser Val Gly Pro Pro Asp Glu Ser His Pro Gly Ser Ser Asp Ser Ser Ala Ser Gln Ser Ser Val Lys Lys Ala Ser Ile Lys Ile Leu Lys Asn Phe Asp Glu Ala Ile Ile Val Asp Ala Ala Ser Leu Asp Pro Glu Ser Leu Tyr Gln Arg Thr Tyr Ala Gly Lys Met Thr Phe Gly Arg Val Ser Asp Leu Gly Gln Phe Ile Arg Glu Ser Glu Pro Glu Pro Asp Val Arg 5g5 600 605 Lys Ser Lys Gly Ser Met Phe Ser Gln Ala Met Lys Lys Trp Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln Ala G1n Tyr Asp Gln Pro Leu Glu Lys Ser Thr Lys Leu <210> 65 <211> 662 <212> PRT
<213> Homo sapiens <400> 65 Met Arg Gly Ala Gly Pro Ser Pro Arg Gln Ser Pro Arg Thr Leu Arg Pro Asp Pro Gly Pro Ala Met Ser Phe Phe Arg Arg Lys Val Lys Gly Lys Glu Gln Glu Lys Thr Ser Asp Val Lys Ser Ile Lys Ala Ser Ile Ser Val His Ser Pro Gln Lys Ser Thr Lys Asn His Ala Leu Leu Glu Ala Ala Gly Pro Ser His Val Ala Ile Asn Ala Ile Ser Ala Asn Met Asp Ser Phe Ser Ser Ser Arg Thr Ala Thr Leu Lys Lys Gln Pro Ser His Met Glu Ala Ala His Phe Gly Asp Leu Gly Arg Ser Cys Leu Asp Tyr Gln Thr Gln Glu Thr Lys Ser Ser Leu Ser Lys Thr Leu Glu Gln Val Leu His Asp Thr Ile Val Leu Pro Tyr Phe Ile Gln Phe Met Glu Leu Arg Arg Met Glu His Leu Val Lys Phe Trp Leu Glu Ala Glu Ser Phe His Ser Thr Thr Trp Ser Arg Ile Arg Ala His Ser Leu Asn Thr Met Lys Gln Ser Ser Leu Ala Glu Pro Val Ser Pro Ser Lys Lys His Glu Thr Thr Ala Ser Phe Leu Thr Asp Ser Leu Asp Lys Arg Leu Glu Asp Ser Gly Ser Ala Gln Leu Phe Met Thr His Ser Glu Gly Ile Asp Leu Asn Asn Arg Thr Asn Ser Thr Gln Asn His Leu Leu Leu Ser Gln Glu Cys Asp Ser Ala His Ser Leu Arg Leu Glu Met Ala Arg Ala Gly Thr His Gln Val Ser Met Glu Thr Gln Glu Ser Ser Ser Thr Leu Thr Val Ala Ser Arg Asn Ser Pro Ala Ser Pro Leu Lys Glu Leu Ser Gly Lys Leu Met Lys Ser Ile Glu Gln Asp Ala Val Asn Thr Phe Thr Lys Tyr Ile Ser Pro Asp Ala Ala Lys Pro Ile Pro Ile Thr Glu Ala Met Arg Asn Asp Ile Ile Ala Arg Ile Cys Gly Glu Asp Gly Gln Val Asp Pro Asn Cys Phe Val Leu Ala Gln Ser Ile Val Phe Ser Ala Met Glu Gln Glu His Phe Ser Glu Phe Leu Arg Ser His His Phe Cys Lys Tyr Gln Ile Glu Val Leu Thr Ser Gly Thr Val Tyr Leu Ala Asp Ile Leu Phe Cys Glu Ser Ala Leu Phe Tyr Phe Ser Glu Tyr Met Glu Lys Glu Asp Ala Val Asn Ile Leu Gln Phe Trp Leu Ala Ala Asp Asn Phe Gln Ser Gln Leu Ala Ala Lys Lys Gly Gln Tyr Asp Gly Gln Glu Ala Gln Asn Asp Ala Met Ile Leu Tyr Asp Lys Tyr Phe Ser Leu Gln Ala Thr His Pro Leu Gly Phe Asp Asp Val Val Arg Leu Glu Ile Glu Ser Asn Ile Cys Arg Glu Gly Gly Pro Leu Pro Asn Cys Phe Thr Thr Pro Leu Arg Gln Ala Trp Thr Thr Met Glu Lys Val Phe Leu Pro Gly Phe Leu Ser Ser Asn Leu Tyr Tyr Lys Tyr Leu Asn Asp Leu Ile His Ser Val Arg Gly Asp Glu Phe Leu Gly Gly Asn Val Ser Pro Thr Ala Pro Gly Ser Val Gly Pro Pro Asp Glu Ser His Pro Gly Ser Ser Asp Ser Ser Ala Ser Gln Ser Ser Val Lys Lys Ala Ser Ile Lys Ile Leu Lys Asn Phe Asp Glu Ala Ile Ile Val Asp Ala Ala Ser Leu Asp Pro Glu Ser Leu Tyr Gln Arg Thr Tyr Ala Gly Lys Met Thr Phe Gly Arg Val Ser Asp Leu Gly Gln Phe Ile Arg Glu Ser Glu Pro Glu Pro Asp Val Arg Lys Ser Lys Gly Ser Met Phe Ser Gln Ala Met Lys Lys Trp Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Val Met Gln Gln Ala Gln Tyr Asp Gln Pro Leu Glu Lys Ser Thr Lys Leu <210> 66 <211> 26 <212> PRT
<213> Homo Sapiens <400> 66 Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 67 <211> 25 <212> PRT
<213> Homo Sapiens <400> 67 Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 68 <211> 24 <212> PRT
<213> Homo Sapiens <400> 68 Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 69 <211> 23 <212> PRT
<213> Homo Sapiens <400> 69 Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 70 <211> 22 <212> PRT
<213> Homo Sapiens <400> 70 Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 71 <211> 21 <212> PRT
<213> Homo Sapiens <400> 71 Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 72 <211> 20 <212> PRT
<213> Homo Sapiens <400> 72 Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 73 <211> 19 <212> PRT
<213> Homo Sapiens <400> 73 Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 74 <211> 18 <212> PRT
<213> Homo Sapiens <400> 74 Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 75 <211> 17 <212> PRT
<213> Homo Sapiens <400> 75 Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 76 <211> 16 <212> PRT
<213> Homo Sapiens <400> 76 Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 77 <211> 15 <212> PRT
<213> Homo Sapiens <400> 77 Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 78 <211> 14 <212> PRT
<213> Homo Sapiens <400> 78 Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 79 <211> 13 <212> PRT
<213> Homo Sapiens <400> 79 Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 80 <211> 12 <212> PRT
<213> Homo Sapiens <400> 80 Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 81 <211> 11 <212> PRT
<213> Homo Sapiens <400> 81 Ala Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 82 <211> 10 <212> PRT
<213> Homo Sapiens <400> 82 Lys Met Ile Val Ser Asp Ile Met Gln Gln <210> 83 <211> 26 <212> PRT
<213> Homo Sapiens <400> 83 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met Gln <210> 84 <211> 25 <212> PRT
<213> Homo Sapiens <400> 84 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile Met <210> 85 <211> 24 <212> PRT
<213> Homo Sapiens <400> 85 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Ile <210> 86 <211> 23 <212> PRT
<213> Homo Sapiens <400> 86 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp <210> 87 <211> 22 <212> PRT
<213> Homo Sapiens <400> 87 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser <210> 88 <211> 21 <212> PRT
<213> Homo Sapiens <400> 88 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val <210> 89 <211> 20 <212> PRT
<213> Homo Sapiens <400> 89 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile <210> 90 <211> 19 <212> PRT
<213> Homo Sapiens <400> 90 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met <210> 91 <211> 18 <212> PRT
<213> Homo Sapiens <400> 91 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys <210> 92 <211> 17 <212> PRT
<213> Homo Sapiens <400> 92 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala <210> 93 <211> 16 <212> PRT
<213> Homo Sapiens <400> 93 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile <210> 94 <211> 15 <212> PRT
<213> Homo Sapiens <400> 94 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys <210> 95 <211> 14 <212> PRT
<213> Homo Sapiens <400> 95 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp <210> 96 <211> 13 <212> PRT
<213> Homo Sapiens <400> 96 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala <210> 97 <211> 12 <212> PRT
<213> Homo Sapiens <400> 97 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu <210> 98 <211> 11 <212> PRT
<213> Homo Sapiens <400> 98 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu <210> 99 <211> 10 <212> PRT
<213> Homo Sapiens <400> 99 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu <210> 100 <211> 27 <212> PRT
<213> Homo Sapiens <400> 100 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Glu Lys Met Ile Trp Ser Asp Val Met Gln Gln <210> 101 <211> 27 <212> PRT
<213> Homo Sapiens <400> 101 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Trp Ser Asp Val Met Gln Gln <210> 102 <211> 17 <212> PRT
<213> Homo Sapiens <220>
<221> ACETYLATION
<222> (1)...(1) <400> 102 Asp Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Val Met Gln Gln <210> 103 <211> 20 <212> PRT
<213> Homo Sapiens <400> 103 Phe Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Trp Ser Asp Val Phe Gln Gln Cys 20 ' , <210> 104 <211> 20~
<212> PRT
<213> Homo Sapiens <400> 104 Phe Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Trp Ser Asp Val Met Gln Gln Cys <210> 105 <211> 20 <212> PRT
<213> Homo Sapiens <400> 105 Gln Glu Glu Phe Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Val Phe Gln Gln Cys <210> 106 <211> 20 <212> PRT
<213> Homo Sapiens <400> 106 Gln Glu Glu Phe Ala Trp Lys Ile Ala Lys Met Ile Ile Ser Asp Val Phe Gln Gln Cys <210> 107 <211> 20 <212> PRT
<213> Homo sapiens <400> 107 Phe Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Ile Ser Asp Val Phe Gln Gln Cys <210> 108 <211> 28 <212> PRT
<213> Homo Sapiens <400> 108 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Val Met Gln Gln Cys <210> 109 <211> 28 <212> PRT
<213> Homo Sapiens <400> 109 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Leu Trp Lys Ile Ala Lys Met Ile Val Ser Asp Val Met Gln Gln Cys 2p 25 <210> 110 <211> 28 <212> PRT
<213> Homo Sapiens <400> 110 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Glu Lys Met Ile Trp Ser Asp Val Met Gln Gln Cys <210> 111 <211> 28 <212> PRT
<213> Homo Sapiens <400> 111 Val Gln Gly Asn Thr Asp Glu Ala Gln Glu Glu Leu Ala Trp Lys Ile Ala Lys Met Ile Trp Ser Asp Val Met Gln Gln Cys <210> 112 <211> 18 <212> PRT
<213> Homo Sapiens <220>
<221> ACETYLATION
<222> (1) . . . (1) <400> 112 Asp Leu Ala Trp Lys Ile Ala Lys Met Ile Val Ser Asp Val Met Gln Gln Cys

Claims (104)

WHAT IS CLAIMED:

1. A polypeptide that is a mutein of a D-AKAP2 polypeptide, wherein the mutein exhibits modified binding to a regulatory subunit of PKA compared to a native D-AKAP2.

2. The polypeptide of claim 1, wherein the native D-AKAP2 comprises a sequence of amino acids set forth as SEQ ID NOs:1 or 2.

3. A polypeptide that is a mutein of a D-AKAP2 polypeptide, wherein the mutein exhibits modified binding to a regulatory subunit of PKA compared to a control.

4. The polypeptide of claim 3, wherein the control is a polypeptide that consists essentially of the sequence of amino acids set forth as SEQ ID
NOs: 1 or 2.

5. The polypeptide of claim 1 or 3 that exhibits enhanced binding to PKA-RI.alpha. subunits.

6. The polypeptide of claim 5 that exhibits normal or reduced binding to PKA-RII.alpha. subunits.

7. The polypeptide of claim 1 or 3 that exhibits enhanced binding to PKA-RII.alpha. subunits relative to PKA-RI.alpha. subunits.

8. The polypeptide of claim 7 that exhibits normal or reduced binding to PKA-RI.alpha. subunits.

9. The polypeptide of claim 1 or 3 that exhibits enhanced binding to both RI.alpha. and RII.alpha. subunits.

10. The polypeptide of claim 9 that exhibits reduced binding to PKA-RI.alpha. subunits.

11. The polypeptide of claim 10 that exhibits normal or increased binding to PKA-RII.alpha. subunits.

12. The polypeptide of claim 1 or 3 that exhibits reduced binding to PKA-RII.alpha. subunits.

13. The polypeptide of claim 12 that exhibits normal or increased binding to PKA-RI.alpha. subunits.

14. The polypeptide of claim 1 or 3 that exhibits reduced binding to both RI.alpha. and RII.alpha. subunits.

15. A peptide comprising between 15 and 39 amino acid residues, wherein the peptide comprises amino acids 7-21 of SEQ ID NOs:1 or 2.

16. The peptide of claim 15, wherein the peptide binds to a regulatory subunit of PKA.

17. The peptide of claim 16, wherein the peptide binds to RII .alpha..

18. The peptide of claim 17, wherein the peptide comprises amino acids 11-24 of SEQ ID NOs:1 or 2 and binds to RI .alpha.subunits.

19. The peptide of claim 1, 3 or 15, wherein the peptide exhibits a preferred or exclusive binding to PKA-RI.alpha. subunits relative to PKA-RII.alpha. subunits;
or enhanced binding to both RI.alpha. and RII.alpha. subunits.

20. The peptide of claim 19, wherein the peptide is selected from the group consisting of peptides that correspond to the substitution in SEQ ID
NOs: 1 or 2 of one, two or more: of Q at residue 9 with F, I, L,V ,H, M, R, T, W or Y;
of L at residue 12 with F, W, or Y; of V at residue 21 with i, I OR W; and of M at residue 25 with F, I, L, T, V, W or Y.

21. The peptide of claim 19, wherein V at residue 21 is substituted with W.

22. The peptide of claim 21, further comprising the substitution of either or both of Q at residue 9 with F, and of M at residue 25 with F.

23. The peptide of claim 1, 3 or 15, wherein the peptide exhibits a preferred or exclusive binding to PKA-RII.alpha. subunits relative to PKA-RI.alpha. subunits.

24. The peptide of claim 23, wherein the peptide is selected from the group consisting of peptides that correspond to the substitution in SEQ ID
NO:2 of one, two or more: of L at residue 12 with A, C, or K; of A at residue 13 with F, H, I, K, L, M or N; of W at residue 14 with C; of K at residue 15 with C;
of K
at residue 18 with C; of M at residue 19 with C; of S at residue 22 with C;
and at D at residue 23 with C.

25. The peptide of claim 1, 3 or 15, wherein the peptide exhibits preferred or exclusive binding to PKA- RII.alpha. subunits to PKA-RI.alpha.
subunits.

26. The peptide of claim 25, wherein the peptide is selected from the group consisting of peptides that correspond to the substitution in SEQ ID
NO:1 of one, two or more: of A at residue 13 with F, H, I, L, M and S; of W at residue 14 with C; of K at residue 15 with C; of K at residue 18 with C; of M at residue 19 with C; of S at residue 22 with C; and of D at residue 23 with C.

27. A peptide having substantially no ability to bind to PKA-RI.alpha. subunit while maintaining the ability to bind to PKA-RII.alpha. subunit, compared to the peptide of SEQ ID NOs:1 or 2, wherein the peptide is selected from the group consisting of:

VQGNTDEAQEELFWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:27) VQGNTDEAQEELIWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:28) VQGNTDEAQEELLWKIAKMIVSD[I/V]MQQ (SEQ ID NO:29) VQGNTDEAQEELAWCIAKMIVSD[I/V]MQQ; (SEQ ID NO:30) VQGNTDEAQEELAWKIACMIVSD[I/V]MQQ; (SEQ ID NO:31) VQGNTDEAQEELAWKIAKCIVAD[I/V]MQQ; (SEQ ID NO:32) and VQGNTDEAQEELAWKIAKMIVCD[I/V]MQQ; (SEQ ID NO:33)

28. The peptide having substantially no ability to bind to a PKA-RI.alpha.
subunit while maintaining a reduced ability to bind to a PKA-RII.alpha. subunit, compared to the peptide of SEQ ID NOs:1 or 2, wherein the peptide is selected from the group consisting of:
VQGNTDEAQEECAWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:34) VQGNTDEAQEEKAWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:35) VQGNTDEAQEELHWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:36) VQGNTDEAQEELKWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:37) VQGNTDEAQEELMWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:38) VQGNTDEAQEELNWLIALMIVSD[I/V]MQQ; (SEQ ID NO:39) VQGNTDEAQEELVWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:40) VQGNTDEAQEELWWKIAKMIVAD[I/V]MQQ; (SEQ ID NO:41) VQGNTDEAQEELYWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:42) VQGNTDEAQEELAWKIAKMIVSC[I/V]MQQ; (SEQ ID NO:43) TDEAQEELAWKIAKMIVSD; (SEQ ID NO:8) DEAQEELAWKIAKMIVS; and (SEQ ID NO:9) EAQEELAWKIAKMIV. (SEQ ID NO:4)

29. A peptide that has enhanced binding to both PKA-RI.alpha. subunit and PKA-RII .alpha. subunit, compared to the peptide of SEQ ID NOs: 1 or 2, wherein the peptide is selected from the group consisting of:

VQGNTDEAFEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:44) VQGNTDEAIEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:45) VQGNTDEALEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:46) VQGNTDEAVEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:47) VQGNTDEAQEELAWKIAKMIVSD[I/V]FQQ; (SEQ ID NO:48) VQGNTDEAQEELAWKIAKMIVSD[I/V]IQQ; (SEQ ID NO:49) and VQGNTDEAQEELAWKIAKMIVSD[I/V]LQQ; (SEQ ID NO:50

30. A peptide that has enhanced ability to bind to PKA-RI.alpha. subunit, while maintaining a normal or reduced ability to bind to PKA-RII.alpha. subunit, compared to the peptide of SEQ ID NOs:1 or 2, wherein the peptide is selected from the group consisting of:

VQGNTDEAQEEFAWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:51) VQGNTDEAQEELAWKIAKMIISD[I/V]MQQ; (SEQ ID NO:52) and VQGNTDEAQEELAWKIAKMILSD[I/V]MQQ; (SEQ ID NO:53)

31. A peptide that has an ability to bind to PKA-RI.alpha. subunit but substantially no ability to bind to PKA-RII.alpha. subunit, compared to the peptide of SEQ ID NOs:1 or 2, wherein the peptide comprises VQGNTDEAQEELAWKIAKMIWSD[I/V]MQQ; (SEQ ID NO:54)

32. A peptide that has an enhanced ability to bind to PKA-RI.alpha. subunit, and a reduced ability to bind to PKA-RII.alpha. subunit, compared to the peptide of SEQ ID NOs: 1 or 2, wherein the peptide has a D-amino acid at the position indicated in bold and wherein the peptide is selected from the group consisting of:
VQGNTTEAQEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:17) VQGNTDEAFEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:18) VQGNTDEAIEELAWKIAKMINSD[I/V]MQQ; (SEQ ID NO:19) VQGNTDEALEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:20) VQGNTDEAVEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:21) VQGNTDEAWEELAWKIAKMIVSD[I/V]MQQ; (SEQ ID NO:22) VQGNTDEAYEELAWKIAKMIVSD[I/V]MQQ;(SEQ ID NO:23) VQGNTDEAQEELAWKIAKMILSD[I/V]MQQ;(SEQ ID NO:24) VQGNTDEAQEELAWKIAKMIVLD[I/V]MQQ;(SEQ ID NO:25) and VQGNTDEAQEELAWKIAKMIVSD[I/V]FQQ.(SEQ ID NO:26)

33. A peptide that has enhanced ability to bind to PKA-RI.alpha. subunit, and a reduced ability to bind to PKA-Rlla subunit, compared to the peptide of SEQ ID NOs:1 or 2, wherein the peptide is selected from the group consisting of:
FEELAWKIAKMIWSDVMQQC; (SEQ ID NO:104; PV-37) FEELAWKIAKMIWSDVFQQC; (SEQ ID NO:103; PV-38) QEEFAWKIAKMIVSDVFQQC; (SEQ ID NO:105; PV-47) QEEFAWKIAKMIISDVFQQC; (SEQ ID NO:106; PV-48).

34. A peptide that has enhanced ability to bind to PKA-RI.alpha. subunit, while maintaining a normal ability to bind to PKA-RII.alpha. subunit, compared to the peptide of SEQ ID NOs:1 or 2, wherein the peptide is:
FEELAWKIAKMIISDVFQQC (SEQ ID NO:107; PV-49).

35. The peptide comprising any one of the peptides of claim 1, wherein when the peptide comprises amino acid positions corresponding to 1-27 of SEQ ID NOs:1 or 2, the peptide further comprises a range of one or more amino acids, up to all of the amino acids, selected from amino acids 1-622 and 650-662 of SEQ ID NOs:64 or 65, wherein the one or more of the selected amino acids are contiguous with amino acid positions corresponding to 623-649 of SEQ, ID NOs:64 or 65.

3C. The peptide of claim 17, wherein the peptide is selected from the group consisting of SEQ ID NOs:4, 8, 9, 76, 77 and 86-88.

37. The peptide of claim 18, wherein the peptide is selected from the group consisting of SEQ ID NOs:S, 6, 7, 66-75 and 83-85.

38. A transgenic non-human knock-in animal comprising a gene encoding an animal homolog of human D-AKAP2, wherein the gene has been modified to encode one, two or more amino acid substitutions in a 27-mer PKA
binding region of the animal homolog of human D-AKAP2 corresponding to amino acids 623-649 of human D-AKAP2 set forth in SEQ ID NOs:63, 64 or 65.

39. The transgenic animal of claim 38, wherein the amino acid substitution in the animal homolog of human D-AKAP2 is selected from the group consisting of substitutions that correspond to the substitution in SEQ
ID
NOs:63, 64 or 65 of one, two or more: of Q at residue 631 with F, I, L, V, H, M, R, T, W or Y; of L at residue 634 with F, W or Y; of V at residue 643 with I, L or W; and of M at residue 647 with F, I, L, T, V, W or Y.

40. The transgenic animal of claim 38 or 39, wherein the modified animal homolog of human D-AKAP2 exhibits a preferred or exclusive binding to PKA-RI.alpha. subunits relative to PKA-RII.alpha. subunits, or enhanced binding to both RI.alpha.
and RII.alpha. subunits, compared to the unmodified animal homolog of human D-AKAP2.

41. The transgenic animal of claim 38, wherein the amino acid substitution in the animal homolog of human D-AKAP2 is selected from the group consisting of substitutions that correspond to the substitution in SEQ
ID
NOs:63, 64 or 65 of one, two or more: of L at residue 634 with A, C, or K; of A
at residue 635 with F, H, I, K, L, M, N or S; of W at residue 636 with C; of K
at residue 637 with C; of K at residue 640 with C; of M at residue 641 with C; of S at residue 644 with C; and of D at residue 645 with C.

42. The transgenic animal of claim 38 or 41, wherein the modified animal homolog of human D-AKAP2 exhibits a preferred or exclusive binding to PKA-RII.alpha. subunits relative to PKA-RII.alpha. subunits, compared to the native unmodified animal homolog of human D-AKAP2.

43. The transgenic animal of claim 38, wherein the animal is a mouse, and wherein the amino acid substitution in the mouse D-AKAP2 is selected from the group consisting of substitutions that correspond to the substitution in SEQ
ID NO:56 of one, two or more: of Q at residue 341 with F, I, L, V, H, M, R, T, W or Y; of L at residue 344 with F, W or Y; of V at residue 353 with I, L or W;
and of M at residue 357 with F, I, L, T, V, W or Y.

44. The transgenic animal of claim 38 or 43, wherein the modified animal homolog of human D-AKAP2 is mouse D-AKAP2 that exhibits a preferred or exclusive binding to PKA-RI.alpha. subunits relative to PKA-RII.alpha.
subunits, or enhanced binding to both RI.alpha. and RII.alpha. subunits, compared to the native unmodified mouse D-AKAP2.

45. The transgenic animal of claim 43, wherein the amino acid substitution in the mouse D-AKAP2 corresponds to a single amino acid substitution in SEQ ID NO:56 of Q at residue 341 with I, and wherein the modified mouse D-AKAP2 exhibits a enhanced binding to PKA-RI.alpha. subunits, and normal binding to PKA-RII.alpha. subunits compared to the native unmodified mouse D-AKAP2.

46. The transgenic animal of claim 43, wherein the amino acid substitution in the mouse D-AKAP2 corresponds to a single amino acid substitution in SEQ ID NO:56 of V at residue 353 with W, and wherein the modified mouse D-AKAP2 exhibits a normal binding to PKA-RI.alpha. subunits, and disrupted binding to PKA-RII.alpha. subunits compared to the native unmodified mouse D-AKAP2.

47. The transgenic animal of claim 43, wherein the amino acid substitution in the mouse D-AKAP2 corresponds to a triple amino acid substitution in SEQ ID N0:56 of Q at residue 341 with F, of V at residue 353 with W, and of M at residue 357 with F, and wherein the modified mouse D-AKAP2 exhibits increased binding to RI.alpha. and decreased binding affinity for RII.alpha..

48. The transgenic animal of claim 47, wherein the modified mouse D-AKAP2 exhibits approximately 10-fold increased binding to RI.alpha. and approximately 220-fold decreased binding affinity for RII.alpha..

49. The transgenic animal of claim 38, wherein the animal is a mouse, and wherein the modified animal homolog of human D-AKAP2 is mouse D-AKAP2 that exhibits a preferred or exclusive binding to PKA-RII.alpha.
subunits relative to PKA-RII.alpha. subunits, compared to the native unmodified mouse D-AKAP2; and wherein the amino acid substitution in the mouse D-AKAP2 is selected from the group consisting of substitutions that correspond to the substitution in SEQ ID NO:56 of one, two or more: of L at residue 344 with A, C, or K; of A at residue 345 with F, H, I, K, L, M, N or S; of W at residue with C; of K at residue 347 with C; of K at residue 350 with C; of M at residue 351 with C; of S at residue 354 with C; and of D at residue 355 with C.

50. The transgenic animal of claim 49, wherein the amino acid substitution in the mouse D-AKAP2 corresponds to a single amino acid substitution in SEQ ID NO:56 of A at residue 345 with L, and wherein the modified mouse D-AKAP2 exhibits normal binding to PKA-RII.alpha. subunits, and disrupted binding to PKA-RI.alpha. subunits compared to the native unmodified mouse D-AKAP2.

51 . A cell, comprising heterologous nucleic acid that encodes a mammalian D-AKAP2 variant protein or portion that exhibits a preferred or exclusive binding to PKA-RI.alpha. subunits relative to PKA-RII.alpha.
subunits; or enhanced binding to both RI.alpha. and RII.alpha. subunits, compared to the unmodified full length D-AKAP2 protein, wherein the D-AKAP2 variant protein or portion thereof comprises at least one single amino acid substitution in the mammalian D-AKAP2 selected from the group consisting of substitutions that correspond to the substitution in SEQ ID NO:63, 64 or 65 of one, two or more: of Q at residue 631 with F, I, L, V, H, M, R, T, W or Y; of L at residue 634 with F, W or Y;
of V
at residue 643 with I, L or W; and of M at residue 647 with F, I, L, T, V, W
or Y.

52. A cell, comprising heterologous nucleic acid that encodes a mammalian D-AKAP2 variant protein or portion that exhibits a preferred or exclusive binding to PKA-RII.alpha. subunits relative to PKA-RI.alpha.
subunits, compared to the unmodified full length D-AKAP2 protein, wherein the D-AKAP2 variant protein or portion thereof comprises at least one single amino acid substitution in the mammalian D-AKAP2 selected from the group consisting of substitutions that correspond to the substitution in SEQ ID NO:63, 64 or 65 of one, two or more: of L at residue 634 with A, C, or K; of A at residue 635 with F, H, I, K, L, M, N or S; of W at residue 636 with C; of K at residue 637 with C; of K at residue 640 with C; of M at residue 641 with C; of S at residue 644 with C;
and of D at residue 645 with C.

53. A cell, comprising heterologous nucleic acid that encodes a mammalian D-AKAP2 variant protein or portion comprising any one of the peptide sequences of claims 1-4 at the corresponding region in the mammalian D-AKAP2 variant protein.

54. A method of disrupting the binding of an Ile/Val(646) isoform of D-AKAP2 corresponding to SEQ ID NOs:64 and 65, or fragment thereof, to the RI.alpha.
subunit of PKA comprising contacting the RI.alpha. subunit with a peptide of claims 1-4 ro 15-18.

55. A method for modulating the amount of PKA-RI.alpha. bound to D-AKAP2 in a cell comprising:
changing the effective intracellular concentration of the Val(646) isoform of D-AKAP2 corresponding to SEQ ID NO:65 in the cell.

56. The method of claim 55, wherein the intracellular concentration of the Val(646) isoform is increased, thereby increasing the amount of PKA-RI.alpha.
bound to D-AKAP2.

57. The method of claim 55, wherein the intracellular concentration of the Val(646) isoform is decreased, thereby decreasing the amount of PKA-RI.alpha.
bound to D-AKAP2.

58. A method for altering the intracellular location of PKA in a cell comprising:
changing the effective intracellular concentration of the Val(646) isoform of D-AKAP2 corresponding to SEQ ID NO:65 in the cell.

59. The method of claim 58, wherein the intracellular concentration of the Val(646) isoform is increased, thereby increasing the amount of PKA
localized to the mitochondria.

60. The method of claim 58, wherein the intracellular concentration of the Val(646) isoform is decreased, thereby decreasing the amount of PKA
localized to the mitochondria.

61. A method of treating a subject manifesting a disease or disorder of signal transduction wherein there is an increased mitochondria) localization of PKA, the method comprising administering a peptide of any of claims 1-4 or peptidomimetic thereof.

62. A method of increasing the longevity of a subject in need thereof, comprising identifying a subject having the 646(Val) isoform of D-AKAP2 therein, and treating the subject with an agent that disrupts the binding the 646(Val) isoform of D-AKAP2 to RI.alpha. subunit of PKA.

63. The method of claim 62, wherein the agent is a mutein of a D-AKAP2 polypeptide, or peptidomimetic thereof.

64. A method for altering the ratio of PKA-RI.alpha./PKA-RII.alpha. bound to D-AKAP2 in a cell comprising;
increasing the concentration of a Val(646) variant of D-AKAP2 corresponding to SEQ ID NO:65 in a cell, thereby increasing the ratio of PKA-RI.alpha.
PKA/PKA-RII.alpha. bound to D-AKAP2 in a cell.

65. A method of decreasing the concentration of cAMP required to stimulate a cAMP mediated signalling pathway, comprising:
increasing the concentration of a Val(646) variant of D-AKAP2 corresponding to SEQ ID NO:65 in a cell.

66. A method of screening for agents that decrease the binding of a Val(646) variant of D-AKAP2 with RI.alpha. PKA, comprising:
combining a candidate agent with a cell comprising a nucleotide sequence which encodes a Val(646) variant D-AKAP protein corresponding to SEQ ID NO:65, operably linked to a promoter such that the nucleotide sequence is expressed as a D-AKAP2 protein in the cell;
and determining the effect of the agent upon the localization of PKA to the mitochondria, wherein a decrease in localization to the mitochondria identifies an agent that decreases the binding of a Val(646) variant of D-AKAP2 with RI.alpha. PKA.

67. A method of screening for agents that decrease the binding of a Val(646) variant of D-AKAP2 to an RI.alpha. subunit of PKA, comprising:
combining a candidate agent with an admixture comprising RI.alpha. and a D-AKAP2 peptide sequence that binds to RI.alpha.; and determining the effect of the agent upon the localization of PKA to a mitochondria.

68. The method of claim 67, wherein the candidate agent is combined with the admixture in a cell-free system.

69. The method of claim 68, wherein the candidate agent is combined with the admixture intracellularly.

70. The method claim 68, wherein the peptide sequence is obtained from any one of claims 1-4 or 15-18.

71. A D-AKAP2 mutein, or a polypeptide fragment thereof, comprising a peptide region corresponding to an A-Kinsase binding (AKB) domain set forth as amino acids 623-649 of SEQ ID NOs:64 or 65, wherein the sequence of the AKB domain peptide region corresponds to any of the peptide sequences of claim 1.

72. The D-AKAP2 mutein of claim 71, having 28 or more amino acid residues, wherein the amino acid sequence of the D-AKAP2 mutein additional to AKB domain peptide region, is 1 to 635 amino acids in length and corresponds to a contiguous region from amino acids 1-622 and/or 650-662 of SEQ ID
NO:64.

73. The D-AKAP2 mutein of claim 71, wherein the mutein is 662 amino acids in length, and further comprises amino acids 1-622 and 650-662 of SEQ ID NO:64.

74. An isolated nucleic acid molecule, comprising a sequence of nucleotides that encodes the D-AKAP2 mutein of claim 71.

75. A nucleic acid vector, comprising the nucleic acid molecule of claim 74.

76. A cell containing the nucleic acid vector of claim 75.

77. A method of producing a D-AKAP2 mutein by growing the cell of claim 76 under conditions whereby the D-AKAP2 mutein is expressed; and isolating the mutein.

78. The method of claim 77, wherein the cell is a mammalian cell, yeast cell, insect cell or bacterial cell.

79. The method of claim 78, wherein the mammalian cell is a human cell.

80. A D-AKAP2 mutein produced by the method of claim 78.

81. A method for identifying a molecule that modulates the biological activity of an D-AKAP2 protein, comprising:
(a) combining the candidate molecule with a cell comprising a nucleotide sequence encoding a D-AKAP2 mutein of claim 71 or portion thereof that retains a biological activity exhibited by a full length variant protein, operably linked to a promoter such that the nucleotide sequence is expressed as an D-AKAP2 mutein or portion thereof in the cell; and (b) determining the effect of the molecule upon the biological activity of the D-AKAP2 mutein or portion thereof.

82. The method of claim 81, wherein the biological activity of the D-AKAP2 mutein or portion thereof is determined by examining signal transduction in the cell.

83. The method of claim 81, wherein the biological activity is the binding of D-AKAP2 protein or portion thereof to protein kinase A.

84. The method of claim 81, wherein the biological activity of the D-AKAP2 protein or portion thereof is determined by examining protein phosphorylation.

85. The polypepeptide or peptide of claim 1, wherein the peptide is 27 amino acids in length.

86. A peptide, comprising between 12 and 39 amino acid residues, wherein the peptide comprises a segment of SEQ ID NOs:1 or 2, wherein the length of the segment ranges from at least amino acids 12-23 of SEQ ID NOs:1 or 2 up to amino acids 1-27 of SEQ ID NOs:1 or 2.

87. The peptide of claim 86, wherein the peptide binds to a regulatory subunit of PKA.

88. The peptide of claim 87, wherein the peptide binds to RII.alpha.
subunits.

89. The peptide of claim 88, wherein the peptide comprises amino acids 1 1-24 of SEQ ID NOs:1 or 2 and binds to RI.alpha. subunits.

90. The peptide of claim 87, wherein the peptide exhibits a preferred or exclusive binding to PKA-RI.alpha. subunits relative to PKA-RII.alpha.
subunits; or enhanced binding to both RI.alpha. and RII.alpha. subunits.

91. The peptide of claim 90, wherein the peptide is selected from the group consisting of peptides that correspond to the substitution in SEQ ID
NOs:1 or 2 of one, two or more: of Q at residue 9 with F, I, L, V, H, M, R, T, W or Y;

of L at residue 12 with F, W or Y; of V at residue 21 with I, L or W; and of M
at residue 25 with F, I, L, T, V, W or Y.

92. The peptide of claim 91, wherein V at residue 21 is substituted with W.

93. The peptide of claim 92, further comprising the substitution of either one or both of Q at residue 9 with F, and of M at residue 25 with F.

94. The peptide of claim 87, wherein the peptide exhibits a preferred or exclusive binding to PKA-RII.alpha. subunits relative to PKA-RI.alpha.
subunits.

95. The peptide of claim 94, wherein the peptide is selected from the group consisting of peptides that correspond to the substitution in SEQ ID
NO:2 of one, two or more: of L at residue 12 with A, C, or K; of A at residue 13 with F, H, I, K, L, M or N; of W at residue 14 with C; of K at residue 15 with C;
of K
at residue 18 with C; of M at residue 19 with C; of S at residue 22 with C;
and of D at residue 23 with C.

96. The peptide of claim 87, wherein the peptide exhibits preferred or exclusive binding to PKA-RII.alpha. subunits relative to PKA-RI.alpha.
subunits.

97. The peptide of claim 96, wherein the peptide is selected from the group consisting of peptides that correspond to the substitution in SEQ ID
NO:1 of one, two or more: of A at residue 13 with F, H, I, L, M and S; of W at residue 14 with C; of K at residue 15 with C; of K at residue 18 with C; of M at residue 19 with C; of S at residue 22 with C; and of D at residue 23 with C.

98. The peptide of claims 86-97, wherein when the peptide comprises amino acid positions corresponding to 1-27 of SEQ ID NO:1 or 2, the peptide further comprises a range of one or more amino acids, up to all of the amino acids, selected from amino acids 1-622 and 650-662 of SEQ ID NOs:64 or 65, wherein the one or more of the selected amino acids are contiguous with amino acid positions corresponding to 623-649 of SEQ ID NOs:64 or 65.

99. A D-AKAP2 mutein, or a polypeptide fragment thereof, comprising a peptide region corresponding to an A-Kinsase binding (AKB) domain set forth as amino acids 623-649 of SEQ ID NOs:64 or 65, wherein the sequence of the AKB domain peptide region corresponds to any of the peptide sequences of claims 86-97.

100. The D-AKAP2 mutein of claim 99, having 28 or more amino acid residues, wherein the amino acid sequence of the D-AKAP2 mutein additional to AKB domain peptide region, is 1 to 635 amino acids in length and corresponds to a contiguous region from amino acids 1-622 and/or 650-662 of SEQ ID
NO:64.

101 . The D-AKAP2 mutein of claim 99, wherein the mutein is 662 amino acids in length, and further comprises amino acids 1-622 and 650-662 of SEQ ID NO:64.

102. An isolated nucleic acid molecule, comprising a sequence of nucleotides that encodes the D-AKAP2 mutein of claim 99.

103. A nucleic acid vector, comprising the nucleic acid molecule of claim 102.

104. A peptide selected from SEQ ID NOs:3-9.