CA2482334A1 - Method and device for trichomonas detection with adhesin as a marker - Google Patents
Method and device for trichomonas detection with adhesin as a marker Download PDFInfo
- Publication number
- CA2482334A1 CA2482334A1 CA002482334A CA2482334A CA2482334A1 CA 2482334 A1 CA2482334 A1 CA 2482334A1 CA 002482334 A CA002482334 A CA 002482334A CA 2482334 A CA2482334 A CA 2482334A CA 2482334 A1 CA2482334 A1 CA 2482334A1
- Authority
- CA
- Canada
- Prior art keywords
- antibody
- sample
- trichomonas
- complex
- adhesin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010037896 heparin-binding hemagglutinin Proteins 0.000 title claims abstract 28
- 238000000034 method Methods 0.000 title claims abstract 19
- 241000224526 Trichomonas Species 0.000 title claims abstract 13
- 238000001514 detection method Methods 0.000 title claims 20
- 239000003550 marker Substances 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 21
- 208000005448 Trichomonas Infections Diseases 0.000 claims abstract 8
- 210000002700 urine Anatomy 0.000 claims abstract 6
- 210000001124 body fluid Anatomy 0.000 claims abstract 4
- 239000010839 body fluid Substances 0.000 claims abstract 4
- 241000224527 Trichomonas vaginalis Species 0.000 claims abstract 3
- 208000015181 infectious disease Diseases 0.000 claims abstract 3
- 238000009595 pap smear Methods 0.000 claims 7
- 239000012491 analyte Substances 0.000 claims 3
- 239000012530 fluid Substances 0.000 claims 3
- 239000012634 fragment Substances 0.000 claims 3
- 238000011144 upstream manufacturing Methods 0.000 claims 3
- 239000003716 antitrichomonal agent Substances 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 2
- 238000003556 assay Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56905—Protozoa
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/44—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from protozoa
Abstract
A method and kit for detecting Trichomonas vaginalis infection in a human subject are disclosed. In the method, a body-fluid sample such as a vaginal-swab sample or urine is obtained from the subject and contacted with an antibody specific against a Trichomonas adhesin peptide, forming an anti body-adhesin peptide complex if the subject is infected with Trichomonas.
The presence or absence of the complex establishes, with a reliability of at least 80%, in the case of a vaginal swab sample, and with a reliability of at least 40% in the case of a urine sample, the presence or absence, respectively, of Trichomonas infection in the subject. A preferred test kit employs a dry-strip, sandwich assay, format.
The presence or absence of the complex establishes, with a reliability of at least 80%, in the case of a vaginal swab sample, and with a reliability of at least 40% in the case of a urine sample, the presence or absence, respectively, of Trichomonas infection in the subject. A preferred test kit employs a dry-strip, sandwich assay, format.
Claims (19)
1. A method for detecting Trichomonas vaginalis infection in a human subject, with a reliability of over 80% in a vaginal swab sample from a female subject and over 40% in a urine sample from the male or female subject, comprising, obtaining a vaginal swab or urine sample from the subject, contacting the sample with an antibody specific against a Trichomonas adhesin peptide, by said contacting, forming an antibody-adhesin peptide complex if the subject is infected with Trichomonas, and detecting the presence or absence of said complex, thereby to detect the presence or absence, respectively, of Trichomonas infection in the subject, with the specified reliability.
2. The method of claim 1, wherein the contacting antibody is immunospecific against an adhesin peptide selected from the group consisting of AP65, AP51, AP33, AP23 adhesin proteins, and immunologically reactive fragments thereof.
3. The method of claim 2, wherein said adhesin peptide is AP65 adhesin protein.
4. The method of claim 2, wherein said antibody is an anti-AP65 antibody.
5. The method of claim 1, wherein said contacting, forming, and detecting steps are effective to detect Trichomonas infection from vaginal samples with a reliability of over 90%. in 68. The method of claim 1, wherein said contacting includes placing the sample on a sample-application zone of a dry strip having, in an upstream to downstream direction, the sample-application region, a reaction zone containing non-immobilized labeled antibody specific against a Trichomonas adhesin peptide, and a detection zone containing immobilized antibody specific against Trichomonas adhesin peptide in said complex, wherein (i) sample migrates in a downstream direction on said strip toward the reaction zone, (ii) adhesin peptide analyte in the sample reacts with non-immobilized antibody in the reaction zone to form a mobile labeled adhesin peptide-antibody complex, and (iii) the complex migrates toward the detection zone, (iv) said complex reacts with the immobilized antibody in the detection zone to form an immobilized labeled complex in the detection zone, and (v) non-complexed nonmobilized labeled antibody migrates downstream of the detection zone, and detecting includes detecting the presence or absence of immobilized labeled complex in the detection zone.
6. The method of claim 1, wherein the sample obtained is a pap smear, said contacting includes forming a pap smear in the presence of labeled antibody, and the method further includes treating the pap smear to remove unbound labeled antibodies.
7. The method of claim 6, wherein the sample obtained and contacted is the residual fluid derived from forming the pap smear.
8. The method of claim 7, wherein the pap smearsample obtained is a liquid based pap smear exemplified by the Cytyc thin-prep pap smear.
9. The method of claim 7, wherein the sample obtained and contacted is the residual fluid derived from forming the liquid based pap smear.
10. The method of claim 1, for detecting Trichomonas infection in a male human subject, wherein said body fluid sample obtained is urine, and said contacting, forming, and detecting steps are effective to detect Trichomonas infection in over 40% of male human subjects infected with Trichomonas.
11. A kit for detecting Trichomonas vaginalis infection in a human subject, with a reliability of over 80% in a vaginal swab sample from a female subject and over 40% in a urine sample from the male or female subject, comprising, a dry-strip capable of wicking a fluid applied thereto by capillarity within the strip, said strip having, in un upstream to downstream direction, a sample-application zone, a reaction zone, and a detection zone said reaction zone containing a non-immobilized labeled antibody specific against a Trichomonas adhesin peptide, effective to form therewith, a mobile adhesin-protein-antibody complex, said detection zone containing immobilized antibody specific against Trichomonas adhesin peptide in said complex, wherein, after application of a body-fluid sample to the sample-application zone, (i) sample migrates in a downstream direction on the strip toward the reaction zone, (ii) adhesin peptide analyte in the sample reacts with non-immobilized antibody in the reaction zone to form a mobile labeled adhesin peptide-antibody complex, (iii) the complex migrates toward the detection zone, (iv) the complex reacts with the immobilized antibody in the detection zone to form an immobilized labeled complex in the detection zone, and (v) non-complexed nonmobilized labeled antibody migrates downstream of the detection zone, allowing the presence or absence of Trichomonas infection in the subject to be determined by the presence or absence of detectable label in the detection zone.
12. The kit of claim 11, wherein the non-immobilized antibody is immunospecific against an adhesin peptide selected from the group consisting of AP65, AP51, AP33, AP23 adhesin proteins, and immunologically reactive fragments thereof.
13. The kit of claim 12, wherein said adhesin peptide is AP65 adhesin protein.
14. The kit of claim 13, wherein the non-immobilized antibody is an anti-AP65 antibody.
15. The kit of claim 14, wherein said antibody is produced by the cell line DM116 or cell line C55
16. A method for detecting Trichomonas infection in a human subject, comprising, obtaining a body-fluid sample from the subject, contacting the sample with a Trichomonas adhesin peptide, by said contacting, forming an antibody-adhesin peptide complex if the subject is infected with Trichomonas, and detecting the presence or absence of said complex, thereby to establish the presence or absence, respectively, of Trichomonas infection in the subject.
17. The method of claim 16, wherein the adhesin peptide selected from the group consisting of AP65, AP51, AP33, AP23 adhesin proteins, and immunologically reactive fragments thereof.
18. The method of claim 17, wherein said adhesin peptide is AP65 adhesin protein.
19. The method of claim 16, wherein said contacting includes placing the sample on a sample-application zone of a dry strip having, in an upstream to downstream direction, the sample-application region, a reaction zone containing non-immobilized Trichomonas adhesin peptide, and effective to react with anti-Trichomonas antibody present in a Trichomonas-infected individual to form a mobile antibody-adhesin peptide complex, and a detection zone containing immobilized antibody specific against human antibody, wherein (i) sample migrates in a downstream direction on said strip toward the reaction zone, (ii) anti-Trichomonas antibody analyte in the sample reacts with labeled, non-immobilized adhesin peptide in the reaction zone to form a mobile labeled adhesin peptide-antibody complex, and (iii) the complex migrates toward the detection zone, (iv) said complex reacts with the immobilized antibody in the detection zone to form an immobilized labeled complex in the detection zone, and (v) non-complexed nonmobilized labeled adhesin migrates downstream of the detection zone, and detecting includes detecting the presence or absence of immobilized labeled complex in the detection zone.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/112,410 US7291477B2 (en) | 2001-07-03 | 2002-03-30 | Method and device for trichomonas detection |
| US10/112,410 | 2002-03-30 | ||
| PCT/US2003/009474 WO2003085403A1 (en) | 2002-03-30 | 2003-03-27 | Method and device for trichomonas detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2482334A1 true CA2482334A1 (en) | 2003-10-16 |
| CA2482334C CA2482334C (en) | 2011-11-22 |
Family
ID=28789774
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2482334A Expired - Lifetime CA2482334C (en) | 2002-03-30 | 2003-03-27 | Method and device for trichomonas detection with adhesin as a marker |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US7291477B2 (en) |
| EP (2) | EP1490684A4 (en) |
| JP (2) | JP4554218B2 (en) |
| KR (1) | KR100995560B1 (en) |
| CN (2) | CN100419427C (en) |
| AU (2) | AU2003226096A1 (en) |
| CA (1) | CA2482334C (en) |
| ES (1) | ES2646176T3 (en) |
| IL (3) | IL164205A0 (en) |
| MX (1) | MXPA04009483A (en) |
| NZ (1) | NZ535615A (en) |
| WO (1) | WO2003085403A1 (en) |
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| US7244398B2 (en) * | 2003-03-21 | 2007-07-17 | S. C. Johnson & Son, Inc. | Device for dispensing a volatile liquid using a wick in an ambient air stream |
| US7285255B2 (en) * | 2002-12-10 | 2007-10-23 | Ecolab Inc. | Deodorizing and sanitizing employing a wicking device |
| US8198505B2 (en) * | 2006-07-12 | 2012-06-12 | The Procter & Gamble Company | Disposable absorbent articles comprising non-biopersistent inorganic vitreous microfibers |
-
2002
- 2002-03-30 US US10/112,410 patent/US7291477B2/en not_active Expired - Lifetime
-
2003
- 2003-03-27 JP JP2003582539A patent/JP4554218B2/en not_active Expired - Lifetime
- 2003-03-27 MX MXPA04009483A patent/MXPA04009483A/en active IP Right Grant
- 2003-03-27 ES ES12181132.7T patent/ES2646176T3/en not_active Expired - Lifetime
- 2003-03-27 EP EP03746064A patent/EP1490684A4/en not_active Ceased
- 2003-03-27 EP EP12181132.7A patent/EP2530466B1/en not_active Expired - Lifetime
- 2003-03-27 CN CNB038123037A patent/CN100419427C/en not_active Expired - Fee Related
- 2003-03-27 CN CNA2008101294810A patent/CN101358971A/en active Pending
- 2003-03-27 NZ NZ535615A patent/NZ535615A/en not_active IP Right Cessation
- 2003-03-27 IL IL16420503A patent/IL164205A0/en active IP Right Grant
- 2003-03-27 AU AU2003226096A patent/AU2003226096A1/en not_active Abandoned
- 2003-03-27 CA CA2482334A patent/CA2482334C/en not_active Expired - Lifetime
- 2003-03-27 WO PCT/US2003/009474 patent/WO2003085403A1/en active Application Filing
- 2003-03-27 KR KR1020047015301A patent/KR100995560B1/en active IP Right Grant
-
2004
- 2004-09-21 IL IL164205A patent/IL164205A/en unknown
-
2007
- 2007-10-11 US US11/870,958 patent/US7879559B2/en not_active Expired - Fee Related
- 2007-10-31 US US11/931,488 patent/US7648829B2/en not_active Expired - Lifetime
-
2009
- 2009-12-17 IL IL202802A patent/IL202802A/en not_active IP Right Cessation
-
2010
- 2010-02-10 AU AU2010200492A patent/AU2010200492B2/en not_active Ceased
- 2010-03-01 JP JP2010044521A patent/JP2010190899A/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| CN101358971A (en) | 2009-02-04 |
| KR100995560B1 (en) | 2010-11-19 |
| EP1490684A1 (en) | 2004-12-29 |
| CN1656381A (en) | 2005-08-17 |
| JP4554218B2 (en) | 2010-09-29 |
| JP2005521888A (en) | 2005-07-21 |
| AU2003226096A1 (en) | 2003-10-20 |
| KR20050008663A (en) | 2005-01-21 |
| US7879559B2 (en) | 2011-02-01 |
| MXPA04009483A (en) | 2005-06-08 |
| US20080085523A1 (en) | 2008-04-10 |
| EP2530466B1 (en) | 2017-09-06 |
| CN100419427C (en) | 2008-09-17 |
| NZ535615A (en) | 2006-05-26 |
| AU2010200492A1 (en) | 2010-03-04 |
| US7291477B2 (en) | 2007-11-06 |
| IL164205A (en) | 2011-01-31 |
| AU2010200492B2 (en) | 2012-10-04 |
| CA2482334C (en) | 2011-11-22 |
| JP2010190899A (en) | 2010-09-02 |
| EP1490684A4 (en) | 2007-04-25 |
| WO2003085403A1 (en) | 2003-10-16 |
| EP2530466A1 (en) | 2012-12-05 |
| US20030073147A1 (en) | 2003-04-17 |
| US20080268466A1 (en) | 2008-10-30 |
| IL202802A (en) | 2013-07-31 |
| ES2646176T3 (en) | 2017-12-12 |
| IL164205A0 (en) | 2005-12-18 |
| US7648829B2 (en) | 2010-01-19 |
| IL202802A0 (en) | 2011-07-31 |
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