CA2473244A1 - High-density lipoprotein assay device and method - Google Patents

High-density lipoprotein assay device and method Download PDF

Info

Publication number
CA2473244A1
CA2473244A1 CA002473244A CA2473244A CA2473244A1 CA 2473244 A1 CA2473244 A1 CA 2473244A1 CA 002473244 A CA002473244 A CA 002473244A CA 2473244 A CA2473244 A CA 2473244A CA 2473244 A1 CA2473244 A1 CA 2473244A1
Authority
CA
Canada
Prior art keywords
hdl
sample
reagent pad
reagent
assay element
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002473244A
Other languages
French (fr)
Other versions
CA2473244C (en
Inventor
Ronald M. Jones
Thomas E. Worthy
Anthony J. Nugent
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alere San Diego Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2473244A1 publication Critical patent/CA2473244A1/en
Application granted granted Critical
Publication of CA2473244C publication Critical patent/CA2473244C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

An assay device and method for measuring the concentration of HDL-associated cholesterol in a blood-fluid sample are described. The assay design is such that removal of non-HDL lipoproteins from a sample and assay of HDL cholesterol in the sample occur without interruption of the assay. The devic e also prevents interference by reagents used for the HDL assay with other assays carried out on the same sample.

Claims (28)

1. An assay device for measuring serum cholesterol associated with high-density lipoproteins (HDL) in a blood fluid sample also containing low density lipoproteins (LDL) or very low density lipoproteins (VLDL), the device comprising:
a sample distribution matrix effective to distribute a blood fluid sample from a sample application region within the matrix to one or more sample collection regions within the matrix;
an HDL assay element, in which HDL concentration can be assayed, spaced apart from said sample distribution matrix, a reagent pad, disposed between said HDL assay element and said sample distribution matrix, and spaced apart from said matrix, said reagent pad containing a reagent effective to selectively remove non-HDL lipoproteins from the fluid sample, and mounting means effective (a) to maintain said device in a sample-distribution position, wherein said HDL assay element and reagent pad are spaced apart from said matrix, and (b) to transfer said device to a test position, whereby the HDL assay element is placed or maintained in contact with the reagent pad, and the reagent pad is, concurrent with or subsequent to said contact, brought into contact with said matrix.
2. The device of claim 1, wherein a lower surface of the HDL assay element is attached to an upper surface of the reagent pad.
3. The device of claim 1, further comprising a cassette body to which said sample distribution matrix is attached.
4. The device of claim 3, further comprising a reaction bar to which said HDL
assay element is attached.
5. The device of claim 4, wherein said mounting means is effective to attach said reaction bar to said cassette body and to adjust the relative positions of the reaction bar and cassette body between said sample-distribution position and said test position.
6. The device of claim 3, wherein said cassette body further comprises a well for containing said blood fluid sample and a sieving pad for removing cellular blood components from said blood fluid sample, in fluid communication with said sample application region.
7. The device of claim 4, further comprising additional assay elements attached to said reaction bar, such that said pads are brought into contact with said sample collection regions when the device is transferred to the testing position.
8. The device of claim 1, wherein said mounting means is further effective to (c) transfer the device from said test position to a position in which said HDL
assay element and reagent pad are spaced apart from said matrix.
9. The device of claim 1, wherein said reaction bar is optically transparent or includes windows through which said assay elements are visible.
10. The device of claim 1, wherein said reagent includes a sulfonated polysaccharide.
11. The device of claim 1, wherein said reagent pad is impregnated with said reagent in a form which is soluble in said fluid sample, and is of a material effective to entrap precipitated non-HDL lipoproteins within the reagent pad.
12. The device of claim 1, wherein said reagent is immobilized to said reagent pad.
13. The device of claim 1, wherein said HDL assay element contains reagents which, in the presence of HDL-associated cholesterol, produce a change in the assay element which can be detected optically.
14. The device of claim 1, wherein said HDL assay element comprises a biosensor.
15. The device of claim 14, wherein said biosensor is effective to measure production of oxygen or hydrogen peroxide which is dependent on HDL-associated cholesterol concentration within said element.
16. The device of claim 1, wherein said reagent pad comprises a porous polymeric membrane.
17. The device of claim 15, wherein said reagent pad is an asymmetric polymeric membrane, having a smaller pored surface and an opposite, larger pored surface.
18. The device of claim 17, wherein said membrane is oriented such that its smaller pored surface faces the HDL assay element.
19. The device of claim 1, wherein each of said HDL assay element and said reagent pad is an asymmetric polymeric membrane, and said membranes are laminated such that the smaller pored surface of the reagent pad contacts a larger pored surface of the HDL
assay element.
20. A method of measuring serum cholesterol associated with high-density lipoproteins (HDL) in a blood fluid sample also containing low density lipoproteins (LDL) or very low density lipoproteins (VLDL), comprising (a) contacting the sample with an absorptive sample distribution matrix through which said sample is distributed to one or more sample collection sites;
(b) bringing into contact with such a sample collection site, a first surface of a reagent pad, to which said sample is transferred, containing a reagent effective to selectively remove non-HDL lipoproteins from the fluid sample, wherein an opposite surface of said reagent pad is in simultaneous contact with an HDL assay element, containing assay reagents effective to produce an indication of HDL
cholesterol concentration, such that successive sample volumes proceed from said reagent pad to said HDL assay element, and (c) determining the level of HDL cholesterol in said sample by detection at said HDL
assay element.
21. The method of claim 20, further comprising the step of breaking said contact between the sample collection site and the first surface of the reagent pad, when a desired amount of sample has been transferred.
22. The method of claim 20, wherein said reagent pad is impregnated with said reagent in a form which is soluble in said fluid sample, and is of a material effective to entrap precipitated non-HDL lipoproteins within the reagent pad.
23. The method of claim 20, wherein said reagent effective to selectively remove non-HDL lipoproteins is immobilized to said reagent pad.
24. The method of claim 22, wherein said reagent pad comprises an asymmetric polymeric membrane, having a smaller pored surface and an opposite, larger pored surface.
25. The method of claim 24, wherein said membrane is oriented such that its smaller pored surface faces said HDL assay element.
26. The method of claim 20, wherein said HDL assay element comprises a biosensor.
27. The method of claim 26, wherein said biosensor is effective to measure production of oxygen or hydrogen peroxide which is dependent on HDL-associated cholesterol concentration within said element.
28. The method of claim 24, wherein each of said HDL assay element and said reagent pad is an asymmetric polymeric membrane, and said membranes are laminated such that the smaller pored surface of the reagent pad contacts a larger pored surface of the HDL assay element.
CA2473244A 2002-01-18 2003-01-17 High-density lipoprotein assay device and method Expired - Lifetime CA2473244C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34992602P 2002-01-18 2002-01-18
US60/349,926 2002-01-18
PCT/US2003/001354 WO2003061569A2 (en) 2002-01-18 2003-01-17 High-density lipoprotein assay device and method

Publications (2)

Publication Number Publication Date
CA2473244A1 true CA2473244A1 (en) 2003-07-31
CA2473244C CA2473244C (en) 2012-02-21

Family

ID=23374556

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2473244A Expired - Lifetime CA2473244C (en) 2002-01-18 2003-01-17 High-density lipoprotein assay device and method

Country Status (13)

Country Link
US (2) US6881581B2 (en)
EP (1) EP1329724B1 (en)
JP (1) JP4379588B2 (en)
KR (1) KR100990888B1 (en)
AT (1) ATE297018T1 (en)
AU (1) AU2003210544B2 (en)
CA (1) CA2473244C (en)
DE (2) DE10204606C1 (en)
DK (1) DK1329724T3 (en)
ES (1) ES2241911T3 (en)
HK (1) HK1057610A1 (en)
PT (1) PT1329724E (en)
WO (1) WO2003061569A2 (en)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10204606C1 (en) * 2002-01-18 2003-10-16 Cholestech Corp Device and method for high density lipoproteins
DE60207196T2 (en) 2002-04-09 2006-07-20 Cholestech Corp., Hayward Method and apparatus for quantification of high density lipoprotein cholesterol
TWI333545B (en) * 2003-04-02 2010-11-21 Cholestech Corp Adhered membranes retaining porosity and biological activity
EP1530045B1 (en) * 2003-11-04 2008-03-05 Cholestech Corporation Reagent-containing membranes and laminates and methods of their preparation
US20050124019A1 (en) * 2003-11-04 2005-06-09 Jones Ronald M. Reagent-containing membranes and laminates and methods of their preparation
DE10352384A1 (en) * 2003-11-10 2005-06-16 Cholestech Corp., Hayward Reagents containing membranes and laminates and process for their preparation
US7435577B2 (en) 2004-02-03 2008-10-14 Polymer Technology Systems, Inc. Direct measurement of chlolesterol from low density lipoprotein with test strip
US20060062688A1 (en) * 2004-02-03 2006-03-23 Polymer Technology Systems, Inc. Bodily fluid analysis system
US7625721B2 (en) * 2004-02-03 2009-12-01 Polymer Technology Systems, Inc. Non-precipitating bodily fluid analysis system
WO2006023679A1 (en) * 2004-08-17 2006-03-02 Polymer Technology Systems, Inc. Apparatus and method for manufacturing bodily fluid test strip
WO2006033733A1 (en) * 2004-08-17 2006-03-30 Polymer Technology Systems, Inc. Bodily fluid analysis system
US8445293B2 (en) 2005-02-09 2013-05-21 Rapid Pathogen Screening, Inc. Method to increase specificity and/or accuracy of lateral flow immunoassays
WO2008012650A2 (en) * 2006-07-25 2008-01-31 Augurix S.A. An immunochromatography device for the diagnosis of diseases in a sample
US7749775B2 (en) 2006-10-03 2010-07-06 Jonathan Scott Maher Immunoassay test device and method of use
US8217025B2 (en) * 2006-11-17 2012-07-10 Harbor Therapeutics, Inc. Drug screening and treatment methods
WO2008086019A1 (en) 2007-01-09 2008-07-17 Cholestech Corporation Device and method for measuring ldl-associated cholesterol
US8609433B2 (en) * 2009-12-04 2013-12-17 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with sample compressor
US8169006B2 (en) 2008-11-29 2012-05-01 Electronics And Telecommunications Research Institute Bio-sensor chip for detecting target material
KR101058743B1 (en) * 2009-06-04 2011-08-24 주식회사 인포피아 Cholesterol Test Strip and Cholesterol Detection Method Using the Same
US9695464B2 (en) 2011-11-11 2017-07-04 Axis-Shield As Blood sample assay method
US9386948B2 (en) 2012-12-05 2016-07-12 Theranos, Inc. Systems, devices, and methods for bodily fluid sample transport
US10248765B1 (en) 2012-12-05 2019-04-02 Theranos Ip Company, Llc Systems, devices, and methods for bodily fluid sample collection, transport, and handling
CN111366442A (en) * 2013-03-15 2020-07-03 赛拉诺斯知识产权有限责任公司 Method and apparatus for sample collection and sample separation
US10371606B2 (en) 2015-07-21 2019-08-06 Theraos IP Company, LLC Bodily fluid sample collection and transport
WO2017044888A1 (en) 2015-09-09 2017-03-16 Theranos, Inc. Methods and devices for sample collection and sample separation
JP6822125B2 (en) * 2016-12-20 2021-01-27 株式会社リコー Inspection equipment and its manufacturing method, inspection kit, transfer medium for inspection equipment, and inspection method
US11857966B1 (en) 2017-03-15 2024-01-02 Labrador Diagnostics Llc Methods and devices for sample collection and sample separation

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4246339A (en) * 1978-11-01 1981-01-20 Millipore Corporation Test device
SE456347B (en) * 1982-02-09 1988-09-26 Ird Biomaterial Ab SURFACE MODIFIED SOLID SUBSTRATE AND PROCEDURES FOR PRODUCING THEREOF
DE3566409D1 (en) * 1984-06-27 1988-12-29 Organon Teknika Bv Binder for low density lipoproteins
US4614077A (en) * 1985-04-17 1986-09-30 K.T. Mfg. Co., Ltd. Automatic tightening method and apparatus
US4839296A (en) * 1985-10-18 1989-06-13 Chem-Elec, Inc. Blood plasma test method
JPS62186940A (en) 1986-02-10 1987-08-15 Kanegafuchi Chem Ind Co Ltd Adsorbent for lipoprotein
US5215666A (en) * 1987-01-12 1993-06-01 Lanxide Technology Company, Lp Ceramic composite and methods of making the same
US4999287A (en) * 1988-05-19 1991-03-12 Chemtrak Corporation Direct measuring assay strip and method of use thereof
US4959324A (en) * 1989-03-16 1990-09-25 Chemtrak, Inc. Sample pad assay initiation device and method of making
US5215886A (en) 1987-06-22 1993-06-01 Patel P Jivan HDL determination in whole blood
US5171688A (en) * 1988-08-30 1992-12-15 Cholestech Corporation Self-corrected assay device
DE4002789C2 (en) * 1989-01-31 2001-01-11 Fuji Photo Film Co Ltd Photographic film cartridge
US5114350A (en) * 1989-03-08 1992-05-19 Cholestech Corporation Controlled-volume assay apparatus
US5087556A (en) * 1989-05-17 1992-02-11 Actimed Laboratories, Inc. Method for quantitative analysis of body fluid constituents
CA2019865A1 (en) * 1989-07-12 1991-01-12 Yatin B. Thakore Device and method for separation of fluid components for component testing
US5135716A (en) * 1989-07-12 1992-08-04 Kingston Diagnostics, L.P. Direct measurement of HDL cholesterol via dry chemistry strips
US5149505A (en) * 1989-07-18 1992-09-22 Abbott Laboratories Diagnostic testing device
DE3929032C2 (en) * 1989-09-01 1998-09-03 Boehringer Mannheim Gmbh Method for the determination of HDL cholesterol by means of a rapid diagnostic with integrated fractionation step
EP0423784B1 (en) * 1989-10-18 1997-01-02 Fuji Photo Film Co., Ltd. Dry analysis element for quantitative analysis of analyte contained in whole blood
US5110724A (en) * 1990-04-02 1992-05-05 Cholestech Corporation Multi-analyte assay device
US5213965A (en) * 1990-07-16 1993-05-25 Cholestech Corporation Solid-phase precipitation assay device
US5213964A (en) * 1990-07-16 1993-05-25 Cholestech Corporation High-density lipoprotein solid-base precipitation assay method
US5258149A (en) * 1990-11-27 1993-11-02 W. R. Grace & Co.-Conn. Process of making a membrane for high efficiency removal of low density lipoprotein-cholesterol from whole blood
DE59207729D1 (en) * 1992-09-11 1997-01-30 Siemens Ag Device for retaining air bubbles
WO1994007146A1 (en) 1992-09-24 1994-03-31 Perseptive Biosystems, Inc. Quantitative measurement of ldl
US5460974A (en) * 1992-10-13 1995-10-24 Miles Inc. Method of assaying whole blood for HDL cholesterol
AU5679894A (en) * 1992-11-30 1994-06-22 Jack L. Aronowitz Dry reagent three element analyte detection system
US5401466A (en) * 1993-06-01 1995-03-28 Miles Inc. Device for the direct measurement of low density lipoprotein cholesterol
US5409664A (en) * 1993-09-28 1995-04-25 Chemtrak, Inc. Laminated assay device
US5543054A (en) * 1993-11-24 1996-08-06 Millipore Corporation Method and apparatus for covalent immobilization of charge- conjugated carbohydrate molecules
US6107045A (en) * 1994-06-30 2000-08-22 Oklahoma Medical Research Foundation Antibodies to lipoproteins and apolipoproteins and methods of use thereof
AU3151495A (en) 1994-08-01 1996-03-04 James T Hsu Lipoprotein cholesterol assays
WO1996015453A1 (en) 1994-11-14 1996-05-23 Spectral Diagnostics Inc. Method and device for determination of proteins employing antigen/antibody reactions
US5728352A (en) * 1994-11-14 1998-03-17 Advanced Care Products Disposable electronic diagnostic instrument
US5695947A (en) * 1995-06-06 1997-12-09 Biomedix, Inc. Amperometric cholesterol biosensor
US5633168A (en) * 1995-06-07 1997-05-27 Glasscock; Larry M. Controlled dispersion flow analysis system
ES2165947T3 (en) * 1995-07-21 2002-04-01 Wako Pure Chem Ind Ltd METHOD FOR MEASURING THE AMOUNT OF A CONSTITUENT CONTAINED IN A SPECIFIC LIPOPROTEIN.
JPH105329A (en) * 1996-06-21 1998-01-13 Kanegafuchi Chem Ind Co Ltd Carrier for adsorbent used for direct blood perfusion and its grain size reduction method
KR100241928B1 (en) * 1997-03-31 2000-03-02 박종근 Determination device in which the electrode is integrally formed on the porous thin film and the quantification method using the same
FI111217B (en) * 1997-06-19 2003-06-30 Nokia Corp Apparatus for sampling
GB9809918D0 (en) 1998-05-08 1998-07-08 Isis Innovation Microelectrode biosensor and method therefor
JP3441993B2 (en) 1999-01-27 2003-09-02 松下電器産業株式会社 Cholesterol sensor
DE10032042A1 (en) * 2000-07-05 2002-01-24 Inventus Biotec Gesellschaft Fuer Innovative Bioanalytik, Biosensoren Und Diagnostika Mbh & Co. Kg Disposable electrochemical biosensor for the quantitative determination of analyte concentrations in liquids
US6596112B1 (en) * 2000-10-20 2003-07-22 Pall Corporation Laminates of asymmetric membranes
JP3686326B2 (en) * 2000-11-08 2005-08-24 アークレイ株式会社 Test piece for measuring high density lipoprotein (HDL) cholesterol
US6844149B2 (en) * 2001-06-29 2005-01-18 International Business Machines Corporation Method, system, and apparatus for measurement and recording of blood chemistry and other physiological measurements
EP2221621B1 (en) * 2001-12-21 2014-05-28 Polymer Technology Systems, Inc. Method for determining HDL cholesterol concentration from whole blood
DE10204606C1 (en) * 2002-01-18 2003-10-16 Cholestech Corp Device and method for high density lipoproteins
DE60207196T2 (en) * 2002-04-09 2006-07-20 Cholestech Corp., Hayward Method and apparatus for quantification of high density lipoprotein cholesterol
CA2499058A1 (en) 2002-09-16 2004-03-25 Polymer Technology Sytems, Inc. Test strip and method for determining ldl cholesterol concentration from whole blood
TWI333545B (en) * 2003-04-02 2010-11-21 Cholestech Corp Adhered membranes retaining porosity and biological activity
US7772007B2 (en) * 2004-04-02 2010-08-10 Cholestech Corporation Assay device for direct measurement of LDL cholesterol

Also Published As

Publication number Publication date
CA2473244C (en) 2012-02-21
EP1329724B1 (en) 2005-06-01
AU2003210544B2 (en) 2008-03-06
EP1329724A2 (en) 2003-07-23
ATE297018T1 (en) 2005-06-15
JP2005515453A (en) 2005-05-26
WO2003061569A2 (en) 2003-07-31
WO2003061569A3 (en) 2003-10-30
EP1329724A3 (en) 2003-09-10
DK1329724T3 (en) 2005-10-03
US20030166291A1 (en) 2003-09-04
KR100990888B1 (en) 2010-11-01
ES2241911T3 (en) 2005-11-01
US7582484B2 (en) 2009-09-01
US20050208609A1 (en) 2005-09-22
DE60204418T2 (en) 2006-03-23
PT1329724E (en) 2005-10-31
DE10204606C1 (en) 2003-10-16
JP4379588B2 (en) 2009-12-09
KR20040090974A (en) 2004-10-27
US6881581B2 (en) 2005-04-19
HK1057610A1 (en) 2004-04-08
DE60204418D1 (en) 2005-07-07

Similar Documents

Publication Publication Date Title
CA2473244A1 (en) High-density lipoprotein assay device and method
US4987085A (en) Blood filtering metering device
AU2005316581B2 (en) Automated immunoassay cassette, apparatus and method
CA2457930A1 (en) Diagnostic testing process and apparatus
HK1082296A1 (en) Adhered membranes retaining porosity and biological activity in assay device for measuring serum cholesterol associated with high-density lipoproteins
EP0806666A3 (en) Method and apparatus for single step assays of whole blood
AU2003210544A1 (en) High-density lipoprotein assay device and method
JP4261369B2 (en) High density lipoprotein assay device and method
WO2005098442A2 (en) Assay system and method for direct measurement of ldl cholesterol
JP2005522681A5 (en)
EP0283613A2 (en) Dry test strip suitable for oxygen demanding detection system
JP3439836B2 (en) Apparatus and method for detecting a subject
DE59907982D1 (en) Method for the detection of a protease activated in apoptosis
WO1996012188A1 (en) Diagnostic method and apparatus for performing the method
JPH04134067U (en) test equipment

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20230117