CA2452712A1 - Methods for detection and quantification of analytes in complex mixtures - Google Patents

Methods for detection and quantification of analytes in complex mixtures Download PDF

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Publication number
CA2452712A1
CA2452712A1 CA002452712A CA2452712A CA2452712A1 CA 2452712 A1 CA2452712 A1 CA 2452712A1 CA 002452712 A CA002452712 A CA 002452712A CA 2452712 A CA2452712 A CA 2452712A CA 2452712 A1 CA2452712 A1 CA 2452712A1
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population
nucleic acid
genedigit
target specific
genedigits
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French (fr)
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CA2452712C (en
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Krassen Dimitrov
Dwayne Dunaway
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Institute for Systems Biology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

The invention provides a diverse population of uniquely labeled probes, containing about thirty or more target specific nucleic acid probes each attached to a unique label bound to a nucleic acid. Also provided is a metho d of producing a population of uniquely labeled nucleic acid probes. The metho d consists of (a) synthesizing a population of target specific nucleic acid probes each having a different specifier; (b) synthesizing a corresponding population of anti-genedigits each having a unique label, the population having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) hybridizing the populations of target nucleic acid probe s to the anti-genedigits, to produce a population in which each of the target specific probes is uniquely labeled. Also provided is a method of detecting a nucleic acid analyte. The method consists of (a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier; (b) contacting the mixture under conditions sufficient for hybridization with a corresponding plurality of anti-genedigits each having a unique label, the plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in the mixture, a target specific probe, and an anti-genedigit.

Claims (87)

1. A diverse population of labels, comprising about thirty or more unique labels, wherein each of said unique labels is bound to a nucleic acid molecule.
2. The diverse population of claim 1, wherein each of said unique labels further comprises about the same unit signal, or a multiple thereof.
3. The diverse population of claim 1, wherein each of said unique labels further comprises a mixture of two or more different labels.
4. The diverse population of claim 1, further comprising unique labels having a diversity selected from a group consisting of about 40, 60, 80, 100, 120 and 140.
5. The diverse population of claim 1, further comprising about 150.
6. The diverse population of claim 1, wherein said labels are fluorescent.
7. A diverse population of uniquely labeled probes, comprising about thirty or more target specific nucleic acid probes each attached to a unique label bound to a nucleic acid.
8. The diverse population of claim 7, wherein each of said unique labels further comprises about the same unit signal, or a multiple thereof.
9. The diverse population of claim 7, wherein each of said unique labels further comprises a mixture of two or more different labels.
10. The diverse population of claim 7, further comprising a diversity of different labels selected from a group consisting of about 50, 100, 200, 500, 1,000, 2,000, 5,000, 1x10 4 and 3x10 4.
11. The diverse population of claim 7, further comprising about 1x10 5.
12. The diverse population of claim 7, wherein said labels are fluorescent.
13. The diverse population of Claim 7, wherein said target specific nucleic acid further comprises said nucleic acid bound to said unique label.
14. The diverse population of Claim 7, further comprising two attached populations of nucleic acids, one population of nucleic acids comprising said thirty or more target specific nucleic acid probes, and a second population of nucleic acids comprising said nucleic acid bound by a unique label.
15. The diverse population of Claim 7, further comprising a nucleic acid species selected from the group consisting of a specifier, an antispecifier, a genedigit, an anti-genedigit and a dendrimer.
16. A method of producing a population of labels, comprising synthesizing a population of nucleic acids, each of said nucleic acids within said synthesized population having bound a predetermined ratio of at least two different labels, wherein said population of labels has a diversity of predetermined ratios of about 30 or more.
17. The method of claim 16, wherein each of said nucleic acids bound by a predetermined ratio of at least two labels further comprises about the same unit signal.
18. The method of claim 16, wherein said diversity is selected from the group consisting of 40, 60, 80, 100, 120, 140, and 150.
19. The method of claim 16, wherein said diversity is selected from the group consisting of 200, 500, 2,000, 5,000, 1x10 4, 3x10 4 and 1x10 5.
20. The method of claim 16, wherein said labels are fluorescent.
21. The method of claim 16, wherein each of said nucleic acids within said synthesized population further comprise an anti-genedigit.
22. The method of claim 16, wherein each of said nucleic acids within said synthesized population further comprise a target specific probe.
23. A method of attaching a label to a nucleic acid probe, comprising hybridizing a nucleic acid probe having a genedigit to an anti-genedigit having a label, said genedigit comprising a set of three or more repeat sequences, said anti-genedigit comprising a cognate set of at least two complimentary repeat sequences, wherein said anti-genedigit specifically hybridizes to said genedigit through a sequence having a complexity less than the number of hybridized base pairs.
24. The method of claim 23, wherein said nucleic acid probe further comprises a specifier.
25. The method of claim 24, wherein said specifier further comprises four or more genedigits.
26. The method of claim 24, wherein said specifier further comprises five or more genedigits.
27. The method of claim 23, wherein said genedigit.
further comprises a set of four or more repeat sequences.
28. The method of claim 23, wherein said anti-genedigit further comprises a cognate set of three or more complimentary repeat sequences.
29. The method of claim 23, wherein said repeat sequences or complimentary repeat sequences further comprise about 8 nucleotides.
30. The method of claim 23, wherein said complexity is about 8.
31. The method of claim 23, wherein said number of hybridized base pairs is about 24.
32. The method of claim 23, further comprising hybridizing a population of nucleic acid probes having different genedigits to a population of different anti-genedigits each having unique labels to produce a population of uniquely labeled nucleic acid probes.
33. The method of claim 32, wherein said nucleic acid probe further comprises a specifier.
34. A method of producing a population of nucleic acid probes, comprising:
(a) producing a first population of nucleic acids comprising two or more target specific probes each having at least one genedigit, said genedigit having a set of three or more repeated sequences;
(b) producing a second population of nucleic acids comprising an anti-genedigit having a cognate set of at least two complimentary repeated sequences, and (c) hybridizing said first and second populations of nucleic acids to produce a population of target specific probes attached to an anti-genedigit, wherein said anti-genedigit hybridizes to said genedigit through a sequence having a complexity less than the number of hybridized base pairs.
35. The method of claim 34, wherein said target specific probes further comprise a specifier.
36. The method of claim 35, wherein said specifier further comprises four or more genedigits.
37. The method of claim 35, wherein said specifier further comprises five or more genedigits.
38. The method of claim 34, wherein said genedigit further comprises a set of four or more repeat sequences.
39. The method of claim 34, wherein said anti-genedigit further comprises a cognate set of three complimentary repeat sequences.
40. The method of claim 34, wherein said repeat sequences or complimentary repeat sequences further comprise about 8 nucleotides.
41. The method of claim 34, wherein said complexity is about 8.
42. The method of claim 34, wherein said number of hybridized base pairs is about 24.
43. The method of claim 34, wherein said two or more target specific probes further comprise at least one different genedigit.
44. The method of Claim 34, wherein said population of anti-genedigits further comprises two or more different anti-genedigits.
45. The method of Claim 34, wherein said anti-genedigit further comprises a label.
46. The method of claim 44, wherein said two or more different anti-genedigits further Comprise unique labels.
47. The method of claim 34, further comprising hybridizing a population of target specific probes having different genedigits to a population of different anti-genedigits each having a unique label to produce a population of uniquely labeled nucleic acid probes.
48. The method of claim 47, wherein said target specific nucleic acid probe further comprises a specifier.
49. A method of producing a population of uniquely labeled nucleic acid probes, comprising:
(a) synthesizing a population of target specific nucleic acid probes each having a different specifier;
(b) synthesizing a corresponding population of anti-genedigits each having a unique label, said population having a diversity sufficient to uniquely hybridize to genedigits within said specifiers, and (c) hybridizing said populations of target nucleic acid probes to said anti-genedigits, to produce a population wherein each of said target specific probes is uniquely labeled.
50. A method of detecting a nucleic acid analyte, comprising:
(a) contacting a mixture of nucleic acid analytes with a plurality of target specific probes each attached to a unique label bound to a nucleic acid under conditions sufficient for hybridization of said probes to said target, and (b) measuring a signal from one or more said target specific probes hybridized to an analyte, wherein said signal uniquely identifies the analyte species.
51. The method of claim 50, further comprising stretching of said target specific probes hybridized to an analyte using a flow-stretch, receding meniscus, or electro-stretch technique, thereby spatially separating labels within a unique label.
52. The method of claim 50, wherein each of said unique labels further comprises about the same unit signal, or multiple thereof.
53. The method of claim 50, wherein each of said unique labels further comprises a mixture of two or more different labels.
54. The method of claim 50, wherein said plurality of target specific probes further comprises different target specific probes selected from the group consisting of about 50, 100, 200, 500, 1,000, 2,000, 5,000, 1x10 4, 3x10 4 and 1x10 5.
55. The method of claim 50, wherein said plurality of target specific probes further comprises at least one target specific probe for each nucleic acid analyte in said mixture.
56. The method of claim 50, wherein said mixture further comprises an expressed RNA population or DNA copy thereof.
57. The method of claim 50, wherein said labels are fluorescent.
58. The method of claim 50, wherein said target specific nucleic acid further comprises said nucleic acid bound to said unique label.
59. The diverse population of claim 50, further comprising two attached populations of nucleic acids, one population of nucleic acids comprising said plurality of target specific nucleic acid probes, and a second population of nucleic acids comprising said nucleic acid bound by a unique label.
60. The diverse population of claim 50, further comprising a nucleic acid species selected from the group consisting of a specifier, an antispecifier, a genedigit, an anti-genedigit and a dendrimer.
61. The method of claim 50, further comprising detecting a single copy of an analyte within said mixture.
62. A method of detecting a nucleic acid analyte, comprising:
(a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a target specific probe having at least one genedigit, said genedigit having a set of three or more repeated sequences;
(b) contacting said mixture under conditions sufficient for hybridization with an anti-genedigit having a cognate set of at least two complimentary repeated sequences, and (c) detecting a hybridized complex comprising said analyte, target specific probe and said anti-genedigit, wherein said anti-genedigit hybridizes to said genedigit through a sequence having a complexity less than the number of hybridized base pairs.
63. The method of claim 62, wherein detecting said hybridized complex comprising said analyte, target specific probe and said anti-genedigit comprises the use of a flow-stretch, receding meniscus, or electro-stretch technique.
64. The method of claim 62, wherein said target specific probe further comprises a specifier.
65. The method of claim 64, wherein said specifier further comprises four or more genedigits.
66. The method of claim 64, wherein said specifier further comprises five or more genedigits.
67. The method of claim 62, wherein said genedigit further comprises a set of four or more repeat sequences.
68. The method of claim 62, wherein said anti-genedigit further comprises a cognate set of three complimentary repeat sequences.
69. The method of claim 62, wherein said repeat sequences or complimentary repeat sequences further comprise about 8 nucleotides.
70. The method of claim 62, wherein said complexity is about 8.
71. The method of claim 62, wherein said number of hybridized base pairs is about 24.
72. The method of claim 62, further comprising two or more target specific probes
73. The method of claim 72, further comprising at least one different genedigit.
74. The method of claim 72, further comprising two or more different anti-genedigits.
75. The method of claim 62, wherein said anti-genedigit further comprises a label.
76. The method of claim 74, wherein said two or more different anti-genedigits further comprise unique labels.
77. The method of claim 62, further comprising a plurality of target specific probes having different genedigits and a plurality of different anti-genedigits each having a unique label.
78. The method of claim 77, wherein said target specific probes further comprise a specifier.
79. A method of detecting a nucleic acid analyte, comprising:
(a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier;
(b) contacting said mixture under conditions sufficient for hybridization with a corresponding plurality of anti-genedigits each having a unique label, said plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within said specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in said mixture, a target specific probe, and an anti-genedigit.
80. The method of claim 79, wherein said uniquely detecting a hybridized complex between one or more analytes in said mixture, a target specific probe, and an anti-genedigit comprises the use of a flow-stretch, receding meniscus, or electro-stretch technique.
81. A nucleic acid labeling kit, comprising a set of genedigits, a set of anti-genedigits and a unique set of labels bound to a nucleic acid.
82. The kit of claim 81, further comprising a specifier.
83. The kit of claim 81, further comprising a dendrimer.
84. The kit of claim 81, wherein said unique label further comprises a ratio of two different labels.
85. The kit of claim 81, wherein said unique label is fluorescent.
86. The kit of claim 81, further comprising a diverse population of unique labels.
87. The kit of claim 86, wherein said diversity is selected from the group consisting of 50, 100, 150, 200, 500, 1,000, 2,000, 5,000, 1x10 4, 3x10 4 and 1x10 5.
CA2452712A 2001-07-03 2002-07-03 Methods for detection and quantification of analytes in complex mixtures Expired - Lifetime CA2452712C (en)

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PCT/US2002/021278 WO2003003810A2 (en) 2001-07-03 2002-07-03 Methods for detection and quantification of analytes in complex mixtures

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CA (3) CA2893908C (en)
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Families Citing this family (208)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7297680B2 (en) 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US7473767B2 (en) * 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
EA012340B1 (en) 2001-10-29 2009-10-30 Круселл Холланд Б.В. Method for producing glycated proteinaceous molecule consisting of a lewis x structure, a sialyl-lewis x structure and/or lacdinac structure
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
WO2003091455A1 (en) * 2002-04-23 2003-11-06 U.S.Genomics, Inc. Compositions and methods related to two-arm nucleic acid probes
WO2004007692A2 (en) * 2002-07-17 2004-01-22 U.S.Genomics, Inc. Methods and compositions for analyzing polymers using chimeric tags
US7632548B2 (en) * 2002-08-02 2009-12-15 Applied Nanotech Holdings, Inc. Remote identification of explosives and other harmful materials
JP4865539B2 (en) 2003-05-09 2012-02-01 クルセル ホランド ベー ヴェー E1 immortalized cell culture and method of culturing the culture to increase the yield of product obtained from the culture
EP2354250A1 (en) 2003-06-23 2011-08-10 Epigenomics AG Methods and nucleic acids for analyses of colorectal cell proliferative disorders
EP2345745A1 (en) 2003-06-23 2011-07-20 Epigenomics AG Methods and nucleic acids for analyses of colorectal cell proliferative disorders
US20040265833A1 (en) * 2003-06-23 2004-12-30 Cathy Lofton-Day Methods and nucleic acids for the analysis of colorectal cell proliferative disorders
US7674628B2 (en) * 2003-08-01 2010-03-09 Applied Nanotech Holdings, Inc. Remote identification of explosives and other harmful materials
US20050048498A1 (en) * 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
US7198900B2 (en) * 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US20050069895A1 (en) * 2003-08-29 2005-03-31 Applera Corporation Compositions, methods, and kits for fabricating coded molecular tags
US20050064435A1 (en) * 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
WO2005050171A2 (en) * 2003-11-17 2005-06-02 U.S. Genomics, Inc. Methods and compositions relating to single reactive center reagents
EP3269826B1 (en) 2003-12-01 2020-03-11 Epigenomics AG Methods and nucleic acids for the analysis of gene expression associated with the development of prostate cell proliferative disorders
DE602004031405D1 (en) * 2003-12-11 2011-03-31 Epigenomics Ag Marker to predict the response to therapy or the survival rate of breast cancer patients
EP1561821B1 (en) 2003-12-11 2011-02-16 Epigenomics AG Prognostic markers for prediction of treatment response and/or survival of breast cell proliferative disorder patients
WO2005067692A2 (en) 2004-01-13 2005-07-28 U.S. Genomics, Inc. Detection and quantification of analytes in solution using polymers
US20050169415A1 (en) * 2004-01-30 2005-08-04 Agere Systems Inc. Timing error recovery system
EP2302073A1 (en) 2004-07-18 2011-03-30 Epigenomics AG Epigenetic methods and nucleic acids for the detection of breast cell proliferative disorders
US8029985B2 (en) * 2004-09-01 2011-10-04 Vybion, Inc. Amplified bioassay
WO2006034879A2 (en) * 2004-09-30 2006-04-06 Epigenomics Ag Epigenetic methods and nucleic acids for the detection of lung cell proliferative disorders
EP2213749A1 (en) * 2004-12-02 2010-08-04 Epigenomics AG Methods and nucleic acids for the analysis of gene expression associated with the prognosis of prostate cell proliferative disorders
US20070009884A1 (en) * 2005-04-11 2007-01-11 Ghc Technologies, Inc. Methods and apparatuses for detecting chemical or biological agents
KR20130021400A (en) * 2005-04-15 2013-03-05 에피제노믹스 아게 Methods and nucleic acids for analyses of cellular proliferative disorders
EP2386654A1 (en) 2005-05-02 2011-11-16 University of Southern California DNA Methylation markers associated with the CpG island methylator phenotype (cimp) in human colorectal cancer
WO2007039128A1 (en) * 2005-09-21 2007-04-12 Epigenomics Ag Markers for the prediction of outcome of anthracycline treatment
WO2007039234A2 (en) 2005-09-29 2007-04-12 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with tissue classification
WO2007039290A2 (en) * 2005-10-03 2007-04-12 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with the prognosis of cell proliferative disorders
US7770717B2 (en) * 2005-10-12 2010-08-10 Scanvaegt International A/S Device for transfer of items
WO2007076132A2 (en) * 2005-12-23 2007-07-05 Nanostring Technologies, Inc. Compositions comprising oriented, immobilized macromolecules and methods for their preparation
AU2013203290B2 (en) * 2005-12-23 2016-05-05 Institute For Systems Biology Nanoreporters and methods of manufacturing and use thereof
JP5537034B2 (en) * 2005-12-23 2014-07-02 ナノストリング テクノロジーズ，インコーポレーテッド Nanoreporter and method for producing and using the same
JP4621595B2 (en) * 2006-01-11 2011-01-26 株式会社東芝 Manufacturing method of semiconductor device
EP2013360A2 (en) 2006-04-17 2009-01-14 Epigenomics AG Methods and nucleic acids for the detection of colorectal cell proliferative disorders
US20090317810A1 (en) * 2006-04-17 2009-12-24 Epigenomics Ag Methods and nucleic acids for the detection of colorectal cell proliferative disorders
US7964347B2 (en) * 2006-06-15 2011-06-21 Krassen Dimitrov Labels for electronic detection of individual molecules and methods for their detection
US7858305B2 (en) * 2006-06-30 2010-12-28 The Invention Science Fund I, Llc Method of combing a nucleic acid
EP2634264B1 (en) 2006-07-21 2016-09-14 Epigenomics AG Methods and nucleic acids related to the gene GLI3 for analyses of cellular proliferative disorders
US20110171637A1 (en) * 2006-07-21 2011-07-14 Reimo Tetzner Methods and nucleic acids for analyses of cellular proliferative disorders
US20090017455A1 (en) * 2006-08-02 2009-01-15 Kwong Gabriel A Methods and systems for detecting and/or sorting targets
AU2007284651B2 (en) 2006-08-09 2014-03-20 Institute For Systems Biology Organ-specific proteins and methods of their use
US20080050724A1 (en) * 2006-08-24 2008-02-28 Microfluidic Systems, Inc. Method of detecting one or more limited copy targets
US7970534B2 (en) 2006-08-24 2011-06-28 Blackbird Technologies, Inc. Mobile unit and system having integrated mapping, communications and tracking
AU2007324702A1 (en) 2006-11-24 2008-05-29 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with the development of prostate cell proliferative disorders
US20090215029A1 (en) * 2007-01-16 2009-08-27 Cytocure, Inc. Methods of isolating and purifying nucleic acid-binding biomolecules and compositions including same
ES2486190T3 (en) 2007-01-19 2014-08-18 Epigenomics Ag Nucleic acids and methods for analysis of cellular proliferative disorders
US20090203011A1 (en) * 2007-01-19 2009-08-13 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
US7843335B2 (en) 2007-03-13 2010-11-30 Blackbird Technologies, Inc. Mobile asset tracking unit, system and method
EP2155899A2 (en) * 2007-04-10 2010-02-24 Nanostring Technologies, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
US20090036324A1 (en) * 2007-07-16 2009-02-05 Rong Fan Arrays, substrates, devices, methods and systems for detecting target molecules
EP2302069A1 (en) 2007-12-11 2011-03-30 Epigenomics AG Methods and nucleic acids for analyses of cell proliferative disorders
US8314052B2 (en) * 2009-03-23 2012-11-20 Base Pair Biotechnologies, Inc. Methods for simultaneous generation of functional ligands
EP2331704B1 (en) 2008-08-14 2016-11-30 Nanostring Technologies, Inc Stable nanoreporters
CN102272600A (en) 2008-12-31 2011-12-07 雅培医护站股份有限公司 Method and device for immunoassay using nucleotide conjugates
ES2669439T3 (en) 2009-06-26 2018-05-25 Epigenomics Ag Methods and nucleic acids for bladder carcinoma analysis
EP2309005B1 (en) 2009-08-03 2015-03-04 Epigenomics AG Methods for preservation of genomic DNA sequence complexity
AU2015261558B2 (en) * 2009-10-13 2017-09-28 Nanostring Technologies, Inc Protein Detection Via Nanoreporters
EP3236264A3 (en) * 2009-10-13 2017-11-08 Nanostring Technologies, Inc Protein detection via nanoreporters
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
CA2779750C (en) 2009-11-06 2019-03-19 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US9371598B2 (en) 2010-04-05 2016-06-21 Prognosys Biosciences, Inc. Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US8735548B2 (en) 2010-06-30 2014-05-27 Amgen Inc. Antibodies which bind to SCNN1A/TNFRSF1A fusion proteins and methods of use thereof
EP2619321B1 (en) * 2010-09-20 2018-08-01 Advanced Cell Diagnostics, Inc. Biomarkers for differentiating melanoma from benign nevus in the skin
ES2687761T3 (en) 2011-01-31 2018-10-29 F. Hoffmann-La Roche Ag Methods of identification of multiple epitopes in cells
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8538373B2 (en) 2011-05-25 2013-09-17 Blackbird Technologies, Inc. Methods and apparatus for emergency tracking
JP6397762B2 (en) 2011-07-08 2018-09-26 エピゲノミクス アーゲー Method for determining prognosis of cancer subject and nucleic acid
EP2758538B1 (en) 2011-09-22 2018-10-31 Lineage Biosciences, Inc. Methods for analyzing heterogeneous samples
WO2013055995A2 (en) 2011-10-14 2013-04-18 President And Fellows Of Harvard College Sequencing by structure assembly
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
EP4108782B1 (en) 2011-12-22 2023-06-07 President and Fellows of Harvard College Compositions and methods for analyte detection
CN104271767B (en) 2012-02-03 2017-08-25 加州理工学院 The coding and decoding of signal in multichannel biochemical measurement
EP3321378B1 (en) 2012-02-27 2021-11-10 Becton, Dickinson and Company Compositions for molecular counting
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
US9758829B2 (en) 2012-06-22 2017-09-12 Htg Molecular Diagnostics, Inc. Molecular malignancy in melanocytic lesions
EP2880184B1 (en) 2012-08-03 2021-03-31 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
EP3578669A1 (en) 2012-08-08 2019-12-11 F. Hoffmann-La Roche AG Increasing dynamic range for identifying multiple epitopes in cells
JP6197039B2 (en) 2012-08-24 2017-09-13 イェール ユニバーシティーＹａｌｅ Ｕｎｉｖｅｒｓｉｔｙ High-throughput multiplex detection system, apparatus and method
EP2917203B1 (en) 2012-11-02 2019-04-03 Dana-Farber Cancer Institute, Inc. Method for identifying myc inhibitors
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
WO2014144806A1 (en) * 2013-03-15 2014-09-18 Institute For Systems Biology Multiplex assay for members of binding pairs
EP2970959B1 (en) 2013-03-15 2018-05-30 Lineage Biosciences, Inc. Methods and compositions for tagging and analyzing samples
EP3013983B1 (en) 2013-06-25 2023-02-15 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
EP3027773B1 (en) 2013-07-30 2020-03-25 President and Fellows of Harvard College Quantitative dna-based imaging and super-resolution imaging
KR102536833B1 (en) 2013-08-28 2023-05-26 벡톤 디킨슨 앤드 컴퍼니 Massively parallel single cell analysis
KR102357699B1 (en) 2013-11-06 2022-02-04 더 유나이티드 스테이츠 오브 어메리카, 애즈 리프리젠티드 바이 더 세크러테리, 디파트먼트 오브 헬쓰 앤드 휴먼 서비씨즈 Method for subtyping lymphoma types by means of expression profiling
US10011811B2 (en) 2013-11-25 2018-07-03 Vericel Corporation Cell seeding device and method
US10240208B2 (en) 2014-03-31 2019-03-26 H. Lee Moffitt Cancer Center And Research Institute, Inc. MicroRNA assay for detection and management of pancreatic cancer precursors
WO2015184461A1 (en) 2014-05-30 2015-12-03 Faruki Hawazin Methods for typing of lung cancer
US10179932B2 (en) 2014-07-11 2019-01-15 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
US11098303B2 (en) 2014-07-30 2021-08-24 President And Fellows Of Harvard College Systems and methods for determining nucleic acids
CN107109471A (en) 2014-08-08 2017-08-29 纳米线科技公司 Method for the mixed cellularity group that deconvoluted using gene expression data
EP3198037A4 (en) * 2014-09-22 2018-03-21 The Regents of the University of California Single molecule rna detection
JP6674951B2 (en) 2014-11-21 2020-04-01 ナノストリング テクノロジーズ，インコーポレイティド Enzyme-free and amplification-free sequencing
WO2016085841A1 (en) 2014-11-24 2016-06-02 Nanostring Technologies, Inc. Methods and apparatuses for gene purification and imaging
CN112782140A (en) 2014-12-03 2021-05-11 伊索普莱西斯公司 Analysis and screening of cellular secretion characteristics
JP2017537645A (en) 2014-12-19 2017-12-21 アプライズ バイオ， インコーポレイテッド Method for identifying multiple epitopes in a selected subpopulation of cells
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
CN107208158B (en) 2015-02-27 2022-01-28 贝克顿迪金森公司 Spatially addressable molecular barcode
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
FI3901281T3 (en) 2015-04-10 2023-01-31 Spatially distinguished, multiplex nucleic acid analysis of biological specimens
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
SG10202107055SA (en) 2015-07-17 2021-08-30 Nanostring Technologies Inc Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
US10640816B2 (en) 2015-07-17 2020-05-05 Nanostring Technologies, Inc. Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
EP3910073B1 (en) 2015-09-01 2024-03-06 Eisai R&D Management Co., Ltd. Splice variants associated with neomorphic sf3b1 mutants
CN108026524A (en) 2015-09-11 2018-05-11 赛卢拉研究公司 Method and composition for nucleic acid library standardization
US11384399B2 (en) 2015-09-29 2022-07-12 Htg Molecular Diagnostics, Inc. Methods for subtyping diffuse large B-cell lymphoma (DLBCL)
CN108368543B (en) 2015-10-12 2023-06-13 领先细胞医疗诊断有限公司 In situ detection of nucleotide variants in high noise samples and related compositions and methods
MX2018005611A (en) 2015-11-03 2018-11-09 Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix.
CN108884495B (en) 2016-02-08 2023-08-22 美国政府(由卫生和人类服务部的部长所代表) Gene markers predict the response of hepatocellular carcinoma to Transcatheter Arterial Chemoembolization (TACE)
CN116083543A (en) 2016-04-01 2023-05-09 克罗玛科德公司 Competitive probes for engineered signal generation
AU2017254645B2 (en) 2016-04-20 2023-02-09 Board Of Regents Of The University Of Nebraska Evaluation of mantle cell lymphoma and methods related thereto
WO2017189525A1 (en) 2016-04-25 2017-11-02 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
JP7131773B2 (en) 2016-04-29 2022-09-06 ボード オブ リージェンツ，ザ ユニバーシティ オブ テキサス システム A targeted measure of transcriptional activity associated with hormone receptors
KR102490693B1 (en) 2016-05-16 2023-01-19 나노스트링 테크놀로지스, 인크. Method for detecting target nucleic acid in a sample
WO2017201165A1 (en) 2016-05-17 2017-11-23 Genecentric Diagnostics, Inc. Methods for subtyping of lung adenocarcinoma
WO2017201164A1 (en) 2016-05-17 2017-11-23 Genecentric Diagnostics, Inc. Methods for subtyping of lung squamous cell carcinoma
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
WO2017218777A1 (en) 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
US11352667B2 (en) 2016-06-21 2022-06-07 10X Genomics, Inc. Nucleic acid sequencing
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
GB2570412A (en) 2016-08-31 2019-07-24 Harvard College Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing
US11460468B2 (en) 2016-09-26 2022-10-04 Becton, Dickinson And Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
EP3538891B1 (en) 2016-11-11 2022-01-05 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
JP6730525B2 (en) 2016-11-21 2020-07-29 ナノストリング テクノロジーズ，インコーポレイティド Chemical composition and method of using the same
US11525783B2 (en) 2016-11-22 2022-12-13 IsoPlexis Corporation Systems, devices and methods for cell capture and methods of manufacture thereof
RU2637623C1 (en) * 2016-12-27 2017-12-05 Федеральное государственное бюджетное образовательное учреждение высшего образования "Российский национальный исследовательский медицинский университет им. Н.И. Пирогова" Министерства здравоохранения Российской Федерации (ФГБОУ ВО РНИМУ им. Н.И. Пирогова Минздрава России) Method for estimation of risk of cancer development in generations of population of radionucleous pollution regions
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
EP3448396A4 (en) 2017-04-14 2020-01-22 President and Fellows of Harvard College Methods for generation of cell-derived microfilament network
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
US10676779B2 (en) 2017-06-05 2020-06-09 Becton, Dickinson And Company Sample indexing for single cells
EP3638814B8 (en) 2017-06-14 2023-06-21 The United States of America, as Represented by The Secretary, Department of Health and Human Services Method for determining lymphoma type
WO2019018764A1 (en) 2017-07-21 2019-01-24 Genecentric Therapeutics, Inc. Methods for determining response to parp inhibitors
US11597965B2 (en) 2017-10-06 2023-03-07 10X Genomics, Inc. RNA templated ligation
US11898210B2 (en) 2017-10-16 2024-02-13 Enterome Tools for assessing FimH blockers therapeutic efficiency
US11946095B2 (en) 2017-12-19 2024-04-02 Becton, Dickinson And Company Particles associated with oligonucleotides
US20190249248A1 (en) 2018-02-12 2019-08-15 Nanostring Technologies, Inc. Biomolecular probes and methods of detecting gene and protein expression
AU2019220440A1 (en) 2018-02-16 2020-09-03 Arc-Net Centre For Applied Research On Cancer Università Degli Studi Di Verona Patient classification and prognostic method
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
WO2019213478A1 (en) 2018-05-04 2019-11-07 Nanostring Technologies, Inc. Gene expression assay for measurement of dna mismatch repair deficiency
CA3099909A1 (en) 2018-05-14 2019-11-21 Nanostring Technologies, Inc. Chemical compositions and methods of using same
JP2021524744A (en) 2018-05-21 2021-09-16 ナノストリング テクノロジーズ，インコーポレイティド Molecular gene signature and how to use it
GB201810190D0 (en) 2018-06-21 2018-08-08 Cancer Research Tech Ltd Prognostic and treatment response predictive method
CN112770776A (en) 2018-07-30 2021-05-07 瑞德库尔有限责任公司 Method and system for sample processing or analysis
JP2022511398A (en) 2018-10-01 2022-01-31 ベクトン・ディキンソン・アンド・カンパニー Determining the 5'transcription sequence
EP3864165A4 (en) 2018-10-09 2022-08-03 Genecentric Therapeutics, Inc. Detecting cancer cell of origin
EP3874057A4 (en) * 2018-11-01 2022-08-17 Advanced Cell Diagnostics, Inc. Methods for digital multiplexing of nucleic acids in situ
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
EP3894590A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
CN113195717A (en) 2018-12-13 2021-07-30 贝克顿迪金森公司 Selective extension in single cell whole transcriptome analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
WO2020154247A1 (en) 2019-01-23 2020-07-30 Cellular Research, Inc. Oligonucleotides associated with antibodies
CA3139791A1 (en) 2019-05-31 2020-12-03 10X Genomics, Inc. Method of detecting target nucleic acid molecules
CN114051534A (en) 2019-07-22 2022-02-15 贝克顿迪金森公司 Single cell chromatin immunoprecipitation sequencing assay
EP4055160B1 (en) 2019-11-08 2024-04-10 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
CN115066501A (en) * 2019-12-19 2022-09-16 阿科亚生物科学股份有限公司 RNA detection
JP7344786B2 (en) * 2019-12-19 2023-09-14 株式会社日立製作所 How to identify any DNA sequence in solution
CN114885610A (en) 2019-12-23 2022-08-09 10X基因组学有限公司 Methods for spatial analysis using RNA templated ligation
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
EP4107284A1 (en) 2020-02-17 2022-12-28 10X Genomics, Inc. In situ analysis of chromatin interaction
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4242325A3 (en) 2020-04-22 2023-10-04 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
CN115605614A (en) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） Primers for immune repertoire profiling
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4162074A1 (en) 2020-06-08 2023-04-12 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4168578A1 (en) 2020-06-18 2023-04-26 Nanostring Technologies, Inc. Compositions and methods for in situ single cell analysis using enzymatic nucleic acid extension
EP4172362A1 (en) 2020-06-25 2023-05-03 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
GB2597332A (en) 2020-07-20 2022-01-26 Institute Of Cancer Res Prognostic and treatment response predictive method
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
GB202015200D0 (en) 2020-09-25 2020-11-11 Institute Of Cancer Res Royal Cancer Hospital The Prognostic and treatment response predictive method
TW202233187A (en) 2020-11-04 2022-09-01 日商衛材R&D企管股份有限公司 Biomarkers for myelodysplastic syndrome (mds) and methods of using the same
WO2022098819A1 (en) * 2020-11-06 2022-05-12 Waters Technologies Corporation Methods and compositions useful for nucleic acid analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
US20220186300A1 (en) 2020-12-11 2022-06-16 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20220235403A1 (en) 2021-01-26 2022-07-28 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
EP4301873A1 (en) 2021-03-03 2024-01-10 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
WO2022266416A1 (en) 2021-06-17 2022-12-22 Nanostring Technologies, Inc. Compositions and methods for in situ single cell analysis using enzymatic nucleic acid extension
CN117651855A (en) 2021-07-13 2024-03-05 10X基因组学有限公司 Method for preparing polymeric substrates with controlled thickness
WO2023015192A1 (en) 2021-08-03 2023-02-09 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
EP4137585A1 (en) 2021-08-20 2023-02-22 Institut D'Investigacions Biomèdiques August Pi I Sunyer - IDIBAPS Cancer informative biomarker signature
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
GB202116745D0 (en) 2021-11-19 2022-01-05 Institute Of Cancer Res Royal Cancer Hospital Prognostic and treatment response predictive method
WO2023131866A1 (en) 2022-01-05 2023-07-13 Eisai R&D Management Co., Ltd. Biomarkers for myelodysplastic syndrome (mds) and methods of using the same
US20230279475A1 (en) 2022-01-21 2023-09-07 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023229988A1 (en) 2022-05-23 2023-11-30 10X Genomics, Inc. Tissue sample mold

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2825529C2 (en) * 1978-06-10 1980-11-13 Manfred Kammer Bar-shaped detergents, in particular soap
GB8405437D0 (en) * 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
US4824775A (en) * 1985-01-03 1989-04-25 Molecular Diagnostics, Inc. Cells labeled with multiple Fluorophores bound to a nucleic acid carrier
US5175270A (en) 1986-09-10 1992-12-29 Polyprobe, Inc. Reagents for detecting and assaying nucleic acid sequences
US4824447A (en) * 1986-12-30 1989-04-25 The United States Of America As Represented By The United States Department Of Energy Enhanced oil recovery system
IL86164A0 (en) * 1987-04-28 1988-11-15 Tamir Biotechnology Ltd Improved dna probes
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5763162A (en) * 1990-03-14 1998-06-09 The Regents Of University Of California Multichromophore fluorescent DNA intercalation complexes
US5401847A (en) 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5320814A (en) 1991-01-25 1994-06-14 Trustees Of Tufts College Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample
WO1994016104A1 (en) * 1993-01-08 1994-07-21 Ctrc Research Foundation Color imaging system for use in molecular biology
US5293050A (en) 1993-03-25 1994-03-08 International Business Machines Corporation Semiconductor quantum dot light emitting/detecting devices
US5543838A (en) 1993-08-31 1996-08-06 Xerox Corporation Signal multiplexing system for an image sensor array
US5679519A (en) * 1995-05-09 1997-10-21 Oprandy; John J. Multi-label complex for enhanced sensitivity in electrochemiluminescence assay
AU6898296A (en) 1995-08-14 1997-03-12 Ely Michael Rabani Methods and devices for parallel multiplex polynucleotide sequencing
AU7398996A (en) 1995-10-11 1997-04-30 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6277583B1 (en) * 1996-02-07 2001-08-21 Conjuchem, Inc. Affinity labeling libraries and applications thereof
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US20020150921A1 (en) 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6387707B1 (en) 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
US7144119B2 (en) 1996-04-25 2006-12-05 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
EP0912761A4 (en) * 1996-05-29 2004-06-09 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
AU4043497A (en) * 1996-07-29 1998-02-20 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6984491B2 (en) * 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6750016B2 (en) * 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) * 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6361944B1 (en) * 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
US20020034737A1 (en) 1997-03-04 2002-03-21 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
EP0979409B1 (en) 1997-02-20 2006-12-27 The Regents of the University of California Plasmon resonant particles, methods and apparatus
US6974669B2 (en) * 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
EP1009852A2 (en) * 1997-09-04 2000-06-21 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
US7348181B2 (en) 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
US7115884B1 (en) 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
CA2306501C (en) * 1997-10-14 2011-03-29 Luminex Corporation Precision fluorescently dyed particles and methods of making and using same
US6207392B1 (en) 1997-11-25 2001-03-27 The Regents Of The University Of California Semiconductor nanocrystal probes for biological applications and process for making and using such probes
JP3468750B2 (en) * 1998-01-22 2003-11-17 ルミネックス コーポレイション Microparticles with multiple fluorescent signals
ATE333499T1 (en) 1998-03-18 2006-08-15 Quark Biotech Inc SELECTION/SUBTRACTION APPROACH TO GENE IDENTIFICATION
AU3555599A (en) * 1998-04-13 1999-11-01 Luminex Corporation Liquid labeling with fluorescent microparticles
EP1091973A4 (en) 1998-06-02 2005-03-23 Univ Yale Multiparametric fluorescence in situ hybridization
EP2045334A1 (en) 1998-06-24 2009-04-08 Illumina, Inc. Decoding of array sensors with microspheres
EP0990903B1 (en) 1998-09-18 2003-03-12 Massachusetts Institute Of Technology Biological applications of semiconductor nanocrystals
US6261779B1 (en) * 1998-11-10 2001-07-17 Bio-Pixels Ltd. Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
EP1006199A1 (en) 1998-12-03 2000-06-07 Kreatech Biotechnology B.V. Applications with and methods for producing selected interstrand crosslinks in nucleic acid
JP4014742B2 (en) 1998-12-04 2007-11-28 キヤノンファインテック株式会社 Sheet processing apparatus and image forming apparatus
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US8367322B2 (en) 1999-01-06 2013-02-05 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
CA2356697C (en) * 1999-01-06 2010-06-22 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6537755B1 (en) * 1999-03-25 2003-03-25 Radoje T. Drmanac Solution-based methods and materials for sequence analysis by hybridization
US6908737B2 (en) 1999-04-15 2005-06-21 Vitra Bioscience, Inc. Systems and methods of conducting multiplexed experiments
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US6534266B1 (en) * 1999-04-22 2003-03-18 Albert Einstein College Of Medicine Of Yeshiva University Assay of transcription sites by multi-fluor fish
EP1179185B1 (en) 1999-05-07 2009-08-12 Life Technologies Corporation A method of detecting an analyte using semiconductor nanocrystals
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
WO2001000875A2 (en) 1999-06-25 2001-01-04 Motorola, Inc. Novel methods and products for arrayed microsphere analysis
WO2001002861A1 (en) * 1999-06-29 2001-01-11 Dako A/S Detection using dendrimers bearing labels and probes
WO2001018524A2 (en) * 1999-08-30 2001-03-15 Illumina, Inc. Methods for improving signal detection from an array
JP2004501340A (en) * 2000-01-13 2004-01-15 ナノスフェアー　インコーポレイテッド Oligonucleotide-attached nanoparticles and methods of use
AU2001236491A1 (en) * 2000-01-18 2003-09-16 Quantom Dot Corporation Oligonucleotide-tagged semiconductor nanocrystals for microarray and fluorescence in situ hybridization
US20020028457A1 (en) * 2000-02-16 2002-03-07 Quantum Dot Corporation Single target counting assays using semiconductor nanocrystals
CA2399189A1 (en) 2000-02-22 2001-08-30 Genospectra, Inc. Microarray fabrication techniques and apparatus
US20030186426A1 (en) 2000-03-15 2003-10-02 The Regents Of The University Of California Multichannel flow cell for interacting single optically trapped, DNA molecules with different chemical species
US6759235B2 (en) 2000-04-06 2004-07-06 Quantum Dot Corporation Two-dimensional spectral imaging system
AU2001278133A1 (en) 2000-08-01 2002-02-13 Surromed, Inc. Methods for solid phase nanoextraction and desorption
AU2002211807A1 (en) 2000-09-11 2002-03-26 Massachusetts Institute Of Technology Direct haplotyping using carbon nanotube probes
US6777244B2 (en) 2000-12-06 2004-08-17 Hrl Laboratories, Llc Compact sensor using microcavity structures
US20020142345A1 (en) 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
EP1379693B1 (en) 2001-03-28 2009-05-20 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified particles
US7473767B2 (en) * 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures

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