CA2450676A1 - Method and system for microfluidic interfacing to arrays - Google Patents

Method and system for microfluidic interfacing to arrays Download PDF

Info

Publication number
CA2450676A1
CA2450676A1 CA002450676A CA2450676A CA2450676A1 CA 2450676 A1 CA2450676 A1 CA 2450676A1 CA 002450676 A CA002450676 A CA 002450676A CA 2450676 A CA2450676 A CA 2450676A CA 2450676 A1 CA2450676 A1 CA 2450676A1
Authority
CA
Canada
Prior art keywords
interface
channel
interface device
substrate
instrument
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002450676A
Other languages
French (fr)
Other versions
CA2450676C (en
Inventor
Michael R. Mcneely
Nils Adey
Mark K. Spute
Edward Ayliffe
Michael Howard
Darin Beutel
John Jensen
Stephen Coffin
Thomas Moyer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2450676A1 publication Critical patent/CA2450676A1/en
Application granted granted Critical
Publication of CA2450676C publication Critical patent/CA2450676C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/50—Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/30—Mixers with shaking, oscillating, or vibrating mechanisms comprising a receptacle to only a part of which the shaking, oscillating, or vibrating movement is imparted
    • B01F31/31—Mixers with shaking, oscillating, or vibrating mechanisms comprising a receptacle to only a part of which the shaking, oscillating, or vibrating movement is imparted using receptacles with deformable parts, e.g. membranes, to which a motion is imparted
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/80—Mixing by means of high-frequency vibrations above one kHz, e.g. ultrasonic vibrations
    • B01F31/86—Mixing by means of high-frequency vibrations above one kHz, e.g. ultrasonic vibrations with vibration of the receptacle or part of it
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00427—Means for dispensing and evacuation of reagents using masks
    • B01J2219/0043—Means for dispensing and evacuation of reagents using masks for direct application of reagents, e.g. through openings in a shutter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00534—Mixing by a special element, e.g. stirrer
    • G01N2035/00544—Mixing by a special element, e.g. stirrer using fluid flow

Abstract

A method and system for providing a fluidic interface to slides bearing microarrays of biomolecules or other samples immobilized thereon to perform a variety of chemical reactions or processing steps on the slide. An interface device seals against the slide to form a chamber or chambers containing all or a portion of the microarray, providing selective access to portions of the slide. The interface device includes inlet and outlet ports permitting liquid sample and reagents to be introduced to and removed from the chamber accessing the slide surface. Pre- and post- array microfluidic circuitry may be included in the interface device or in attachable modules. The system may include one or more compartments for collecting and storing waste fluids.

Claims (73)

1. An interface device for interfacing to a surface of a substrate bearing a biological sample immobilized on a first surface thereof, comprising:
a. a main interface layer having an interface surface adapted to conform to the first surface of the substrate;

b. a gasket positioned at said interface surface and having at least one opening formed therethrough, said opening defining an interface channel;
c. an inlet channel formed in said main interface layer and opening into said at least one opening;

d. an outlet channel formed in said main interface layer and opening into said at least one opening; and e. at least one air bladder formed in said main interface layer adjacent said interface surface, wherein said at least one air bladder has a flexible lower surface capable of being deflected outward as said air bladder is inflated or inward as said air bladder is deflated, and wherein said air bladder communicates with at least one air channel that passes through said main interface layer and is adapted for connection with a pressure source.
2. The interface device of claim 1, wherein said gasket defines an interface channel having a height of between about 20 µm and about 30 µm.
3. The interface device of claim l, wherein said gasket defines an interface channel having a height of between about 23 µm and about 27 µm.
4. The interface device of claim 1, wherein said flexible lower surface of said at least one air bladder comprises a flexible diaphragm layer attached to the lower surface of said main interface layer.
5. The interface device of claim 1, wherein said flexible lower surface of said at least one air bladder comprises a thin layer of the rigid material forming the bulk of said main interface layer.
6. The interface device of claim 1, further comprising a waste reservoir in said main interface layer in fluid communication with said at least one outlet channel.
7. The interface device of claim 1, further comprising a microfluidic circuit comprising at least one of a mixing circuit, a branching structure, a reservoir, a valve, or a stop junction.
8. The interface device of claim 7, wherein said microfluidic circuit is in fluid communication between said inlet channel and said at least one interface channel.
9. The interface device of claim 7, wherein said microfluidic circuit is in fluid communication between said at least one interface channel and said outlet.
10. The interface device of claim 7, wherein said microfluidic circuit comprises at least one passive valve.
11. The interface device of claim 1, wherein said at least one interface channel is defined at least in part by an interface well or groove formed in said interface surface of said main interface layer.
12. The interface device of claim 1, wherein said gasket is formed from a resilient sheet material.
13. The interface device of claim 1, wherein said gasket is formed by application of said resilient material directly to said interface surface.
14. The interface device of claim 1, wherein said gasket is formed of an elastic material.
15. The interface device of claim 1, wherein said gasket is formed of a plastically deformable material.
16. The interface device of claim 1, wherein said gasket is formed of an adhesive material.
17. There interface device of claim 16, wherein said adhesive is adapted to adhere reversibly to said first surface of said substrate.
18. The interface device of claim of 1, wherein said interface device is constructed from multiple layers, and wherein said interface device further comprises microfluidic circuit structures formed in at least two of said multiple layers.
19. The interface device of claim 1, wherein said substrate is a microarray slide.
20. An interface device for interfacing to a surface of a substrate bearing biological samples immobilized on a first surface thereof, comprising:

a. a main interface layer having an interface surface adapted to conform to the first surface of the substrate;

b. a gasket positioned at said interface surface and having a plurality of openings formed therethrough, each said opening defining an interface channel or chamber;

c. at least one inlet channel formed in said main interface layer and opening into each said opening; and d. at least one outlet channel formed in said main interface layer and opening into each said opening.
21. The interface device of claim 20, further comprising at least one air bladder formed in said main interface layer adjacent said interface surface, wherein said at least one air bladder has a flexible lower surface capable of being deflected outward as said air bladder is inflated or inward as said air bladder is deflated, and wherein said air bladder communicates with at least one said air channel that passes through said main interface layer and is adapted for connection with a pressure source.
22. The interface device of claim 20, further comprising a microfluidic branching structure in fluid communication between one said inlet and a plurality of said interface channels, said branching structure adapted to divide fluid entering said inlet among said plurality of interface channels.
23. The interface device of claim 20, further comprising a microfluidic circuit in fluid communication between at least two said interface channels and one said outlet, said microfluidic circuit adapted to combine fluids from said at least two interface channels into a single fluid stream flowing through said outlet.
24. The interface device of claim 20, wherein at least one said interface channel is defined at least in part by an interface well or groove formed in said interface surface of said main interface layer.
25. The interface device of claim 20, wherein said gasket is formed from a resilient sheet material.
26. The interface device of claim 20, wherein said gasket is formed by application of said resilient material directly to said interface surface.
27. The interface device of claim 20, wherein said gasket is formed of an elastic material.
28. The interface device of claim 20, wherein said gasket is formed of a plastically deformable material.
29. The interface device of claim 20, wherein said gasket is formed of an adhesive material.
30. The interface device of claim 29, wherein said adhesive is adapted to adhere reversibly to said first surface of said substrate.
31. The interface device of claim 20, wherein said interface device is formed of multiple layers, and wherein said interface device further comprises a 3D microfluidic circuit comprising microfluidic circuit components in at least two of said multiple layers.
32. The interface device of claim 31, wherein 3D microfluidic circuit comprises overlapping microfluidic circuit structures.
33. The interface device of claim 20, wherein said substrate is a microarray slide.
34. An instrument for processing a substrate bearing a biological sample immobilized on a first surface thereof, comprising:

a. a heat block;

b. a base comprising least one recess in thermal communication with said heat block and adapted to receive a reaction device comprising:

i. a substrate bearing a biological sample immobilized on a first surface thereof; and ii. an interface device adapted to seal to said first surface to form at least one interface channel or well containing said biological sample;

c. a heater controller adapted to send a temperature control signal to said heat block;

d. a mixing element located in at least one of said instrument and said interface device capable of producing agitation of fluid in said interface channel; and e. a mixing controller adapted to send a mixing control signal to said mixing element.
35. The instrument of claim 34, wherein said mixing element comprises at least one air bladder located in said interface device adjacent said at least one interface channel, and wherein said mixing controller controls the generation of positive and negative air pressures used to inflate and deflate said air bladder
36. The instrument of claim 34, wherein said mixing element comprises a piezoelectric element, and wherein said mixing controller controls the generation of a voltage signal that causes activation of said piezoelectric element.
37. The instrument of claim 34, wherein said interface device further comprises at least one active element having an electrical connection, and wherein said instrument further comprises at least one additional component adapted to send or receive electrical signals from said at least one active element.
38. The instrument of claim 34, wherein said active element comprises an electrode, and wherein said at least one additional component sends an electrical signal to said electrode.
39. The instrument of claim 34, wherein said active element comprises a sensor, and wherein said at least one additional component receives an electrical signal from said sensor.
40. The instrument of claim 34, further comprising an adaptor for making a fluid connection to said reaction device, whereby fluid may be delivered to said interface channel or well of said reaction device from said instrument.
41. The instrument of claim 34, comprising a plurality of recesses, each recess adapted to receive one said reaction device.
42. The instrument of claim 41, further comprising a plurality of adaptors for making fluid connections to reaction devices received in said plurality of recesses, whereby fluid may be delivered to said interface charnel or well of each said reaction device from said instrument.
43. An instrument for processing a substrate bearing a biological sample immobilized on a first surface thereof, comprising:

a. a heat block adapted to fit into laboratory heater;

b. at least one recess in thermal communication with said heat block and adapted to receive a reaction device comprising:

i. a substrate bearing a biological sample immobilized on a first surface thereof; and ii. an interface device adapted to seal to said first surface to form at least one interface channel containing said biological sample.
44. The instrument of claim 43, further comprising:

a. a mixing element located in at least one of said instrument and said interface device, said mixing element capable of producing agitation of fluid in said interface channel; and b. a mixing controller adapted to send a mixing control signal to said mixing element.
45. The instrument of claim 44, wherein said mixing element comprises at least one air bladder located in said interface device adjacent said at least one interface channel, and wherein said mixing controller controls the generation of positive and negative air pressures used to inflate and deflate said air bladder.
46. The instrument of claim 45, wherein said mixing element comprises two air bladders located at opposite ends of said at least one interface channel, wherein said mixing controller inflates and deflates said two air bladders reciprocally to produce agitation of fluid in said at least one interface channel.
47. The instrument of claim 44, wherein said mixing element comprises a piezoelectric element, and wherein said mixing controller controls the generation of a voltage signal that causes activation of said piezoelectric element.
48. A reaction device for providing controlled delivery of fluids to the surface of a substrate bearing an immobilized biological sample, comprising:

a. a substrate bearing an immobilized biological sample on a first surface thereof;

b. an interface device adapted to seal to said first surface of said substrate to form at least one interface channel containing said biological sample, said interface device comprising i. a main interface layer formed of a rigid material and having an interface surface adapted to conform to said first surface of the substrate;

ii. a gasket positioned at said interface surface and having at least one opening formed therethrough, said opening defining an interface channel or chamber;

iii. an inlet channel passing through said main interface layer and opening into said at least one opening; and iv. an outlet channel passing through said main interface layer and opening into said at least one opening.
49. The reaction device of claim 48, wherein said substrate comprises a microarray slide.
50. The reaction device of claim 48, wherein said gasket defines an interface channel or chamber having a height of between about 20 µm and about 30 µm.
51. The reaction device of claim 48, wherein said gasket defines an interface channel or chamber having a height of between about 23 µm and about 27 µm.
52. The reaction device of claim 48, further comprising a base having a recess adapted to receive said substrate.
53. The reaction device of claim 52, further comprising a clamp mechanism for clamping said base, substrate, and interface device together to seal said interface device to said substrate.
54. The reaction device of claim 53, wherein said clamp mechanism comprises two c-channel clamps pivotally mounted to said base, wherein said c-channel clamps pivot laterally outward in the plane of said substrate received in said base.
55. The reaction device of claim 48, wherein said base comprises a part of an instrument adapted for processing microarray slides, and wherein said instrument further comprises a clamp mechanism adapted to press said interface device and said substrate against said base.
56. The reaction device of claim 48, further comprising an external control module.
57. The reaction device of claim 48, wherein said base further comprises an active element selected from a heating element and a piezoelectric mixing element.
58. The reaction device of claim 48, further comprising a clamp mechanism adapted to hold said interface device in sealing relationship with said substrate.
59. The reaction device of claim 48, further comprising at least one air bladder formed in said main interface layer adjacent said interface surface, wherein said at least one air bladder has a flexible lower surface capable of being deflected outward as said air bladder is inflated or inward as said air bladder is deflated, and wherein said air bladder communicates with at least one air channel that passes through said main interface layer and is adapted for connection with a pressure source.
60. The reaction device of claim 59, wherein said flexible lower surface of said at least one air bladder comprises a flexible diaphragm layer attached to the lower surface of said main interface layer.
61. The reaction device of claim 59, wherein said flexible lower surface of said at least one air bladder comprises a thin layer of the rigid material forming the bulk of said main interface layer.
62. The reaction device of claim 48, wherein said interface device further comprises a waste reservoir in said main interface layer in fluid communication with said at least one outlet channel.
63. The reaction device of claim 48, wherein said at least one opening defines a loop channel having a loop configuration, wherein fluid is circulated through said loop channel, and wherein circulation of fluid into and around said loop is regulated by passive valves.
64. The reaction device of claim 48, wherein a first portion of said reaction device is disposable and a second portion of said reaction device is reusable.
65. The reaction device of claim 52, wherein said base is adapted to seal to said interface device to form a humidity chamber around said substrate, whereby said seal between said substrate and said interface device may be exposed to a humid environment.
66. The reaction device of claim 48, wherein the volume of said interface channel or chamber is reduced by flowing a filler material into said interface channel or chamber to fill a portion of the volume of said interface channel or chamber opposite said substrate, wherein said filler material is immiscible with fluid to be introduced to said interface channel or chamber.
67. A method of performing a chemical reaction with molecules immobilized on a substrate, comprising the steps of:
a. providing a substrate bearing immobilized molecules thereon;
b. positioning an interface device with respect to said substrate, said interface device comprising an inlet port and an outlet port;
c. clamping said interface device to said substrate, thus sealing said interface device to said substrate to form an interface channel having a first end and a second end at the interface between said interface device and said substrate, wherein said interface channel communicates with said inlet port at said first end and with said outlet port at said second end and wherein said interface channel contains at least a portion of said substrate bearing immobilized molecules;
d. introducing a fluid containing a reactant to said interface channel via an inlet port of said interface device; and e. reacting said reactant in said fluid with said immobilized molecules in said interface channel.
68. A method of performing a binding reaction between two chemical compounds, comprising the steps of:
a. providing a substrate having a first compound immobilized thereon;
b. sealing an interface device against said substrate to form an interface channel having an inlet and an outlet and comprising a portion of said substrate having said first chemical compound immobilized thereon, said interface device comprising:
i. at least one inlet channel passing through said interface device and communicating with said interface channel via said inlet;
c. introducing a solution containing a blocking solution into interface channel via said inlet channel;
d. incubating said blocking solution on said portion of said substrate to block non-specific binding sites on said substrate;
e. introducing a first wash solution into said interface channel via said inlet channel, wherein said blocking solution exits said interface channel via said outlet as said first wash solution enters said interface channel;

f. introducing a binding solution containing a second chemical compound into said interface channel via said inlet channel, wherein said wash solution exits said interface channel via said outlet as said binding solution enters said interface channel;
g. incubating said binding solution on said portion of said substrate to produce binding of said second chemical compound to said first chemical compound;
and h. introducing a second wash solution into said interface channel via said inlet channel, wherein said binding solution exits said interface channel via said outlet as said second wash solution enters said interface channel.
69. The method of claim 68, further comprising a pre-processing step of labeling said second chemical compound in said binding solution prior to introduction of said binding solution into said inlet channel.
70. The method of claim 68, further comprising a pre-processing step of purifying impurities out of said binding solution prior to introduction of said binding solution into said inlet channel.
71. The method of claim 68, wherein said first chemical compound comprises a first polynucleotide, wherein said second chemical compound comprises a second nucleotide, wherein said blocking solution is a pre-hybridization buffer, and wherein binding of said second chemical compound to said first chemical compound comprises a hybridization reaction.
72. The method of claim 68, further comprising the step of detecting binding of said first chemical compound and said second chemical compound on said substrate.
73. The method of claim 68, wherein mixing of fluid within said interface channel is provided during at least one of said method steps.
CA2450676A 2001-03-09 2002-03-08 Method and system for microfluidic interfacing to arrays Expired - Fee Related CA2450676C (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US27438901P 2001-03-09 2001-03-09
US60/274,389 2001-03-09
US28442701P 2001-04-17 2001-04-17
US60/284,427 2001-04-17
US31370301P 2001-08-20 2001-08-20
US60/313,703 2001-08-20
US33985101P 2001-12-12 2001-12-12
US60/339,851 2001-12-12
PCT/US2002/007113 WO2002072264A1 (en) 2001-03-09 2002-03-08 Method and system for microfluidic interfacing to arrays

Publications (2)

Publication Number Publication Date
CA2450676A1 true CA2450676A1 (en) 2002-09-19
CA2450676C CA2450676C (en) 2010-03-30

Family

ID=27501106

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2450676A Expired - Fee Related CA2450676C (en) 2001-03-09 2002-03-08 Method and system for microfluidic interfacing to arrays

Country Status (6)

Country Link
US (3) US7223363B2 (en)
EP (1) EP1372848A4 (en)
JP (1) JP4148778B2 (en)
KR (1) KR100916074B1 (en)
CA (1) CA2450676C (en)
WO (1) WO2002072264A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014083799A1 (en) * 2012-11-27 2014-06-05 日本電気株式会社 Fluidic chip and waste liquid processing method for same
US11268632B2 (en) 2013-09-09 2022-03-08 Efficient Robotics Gmbh Microfluidic analysis component and production method

Families Citing this family (399)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
US6677160B1 (en) * 1999-09-29 2004-01-13 Pharmacia & Upjohn Company Methods for creating a compound library and identifying lead chemical templates and ligands for target molecules
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
CA2290731A1 (en) * 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
US7867763B2 (en) 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
US20050118073A1 (en) * 2003-11-26 2005-06-02 Fluidigm Corporation Devices and methods for holding microfluidic devices
US6890093B2 (en) * 2000-08-07 2005-05-10 Nanostream, Inc. Multi-stream microfludic mixers
EP1309404A2 (en) * 2000-08-07 2003-05-14 Nanostream, Inc. Fluidic mixer in microfluidic system
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
WO2002072264A1 (en) * 2001-03-09 2002-09-19 Biomicro Systems, Inc. Method and system for microfluidic interfacing to arrays
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7829025B2 (en) * 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US20050009101A1 (en) * 2001-05-17 2005-01-13 Motorola, Inc. Microfluidic devices comprising biochannels
DE50212817D1 (en) * 2001-05-25 2008-11-06 Tecan Trading Ag Device and process unit for providing a hybridization space
US20030108664A1 (en) * 2001-10-05 2003-06-12 Kodas Toivo T. Methods and compositions for the formation of recessed electrical features on a substrate
DE10149684B4 (en) * 2001-10-09 2005-02-17 Clondiag Chip Technologies Gmbh Device for holding a substance library carrier
DE10150549A1 (en) * 2001-10-12 2003-04-17 Roche Diagnostics Gmbh Separation module, useful for the separation of corpuscles from blood, comprises two channels from a junction with a faster flow in one channel taking most of the particles, and a slower flow with few particles through the other channel
US8053249B2 (en) * 2001-10-19 2011-11-08 Wisconsin Alumni Research Foundation Method of pumping fluid through a microfluidic device
WO2003036298A2 (en) * 2001-10-25 2003-05-01 Exiqon A/S Closed substrate platforms suitable for analysis of biomolecules
US20030138969A1 (en) * 2002-01-24 2003-07-24 Jakobsen Mogens Havsteen Closed substrate platforms suitable for analysis of biomolecules
US7770436B2 (en) * 2001-10-31 2010-08-10 Rheosense, Inc. Micro rheometer for measuring flow viscosity and elasticity for micron sample volumes
US6877892B2 (en) * 2002-01-11 2005-04-12 Nanostream, Inc. Multi-stream microfluidic aperture mixers
US6976590B2 (en) * 2002-06-24 2005-12-20 Cytonome, Inc. Method and apparatus for sorting particles
US9943847B2 (en) 2002-04-17 2018-04-17 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US20070065808A1 (en) * 2002-04-17 2007-03-22 Cytonome, Inc. Method and apparatus for sorting particles
US6808075B2 (en) 2002-04-17 2004-10-26 Cytonome, Inc. Method and apparatus for sorting particles
AU2003228642A1 (en) * 2002-04-23 2003-11-10 Exegetics, Inc. System and method for collecting dna and fingerprints
US20060199260A1 (en) * 2002-05-01 2006-09-07 Zhiyu Zhang Microbioreactor for continuous cell culture
US20040077075A1 (en) * 2002-05-01 2004-04-22 Massachusetts Institute Of Technology Microfermentors for rapid screening and analysis of biochemical processes
EP2278338B1 (en) * 2002-05-09 2020-08-26 The University of Chicago Device and method for pressure-driven plug transport and reaction
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
US20030217923A1 (en) * 2002-05-24 2003-11-27 Harrison D. Jed Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US20030232344A1 (en) * 2002-06-14 2003-12-18 Arthur Schleifer Hybridization process for arrays
US20060120921A1 (en) * 2002-06-20 2006-06-08 Stuart Elliot Biological reaction apparatus with draining mechanism
EP1385006A3 (en) * 2002-07-24 2004-09-01 F. Hoffmann-La Roche Ag System and cartridge for processing a biological sample
US7214348B2 (en) * 2002-07-26 2007-05-08 Applera Corporation Microfluidic size-exclusion devices, systems, and methods
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
JP3824160B2 (en) * 2002-08-28 2006-09-20 株式会社島津製作所 High-speed liquid chromatograph mixer
US7279134B2 (en) * 2002-09-17 2007-10-09 Intel Corporation Microfluidic devices with porous membranes for molecular sieving, metering, and separations
WO2004034056A2 (en) * 2002-10-08 2004-04-22 Tara Nylese Portable diagnostic device and method for determining temporal variations in concentrations
JP3746756B2 (en) * 2002-10-22 2006-02-15 株式会社日立製作所 Solution stirring device, solution stirring method
GB2395006A (en) * 2002-10-29 2004-05-12 Micro Chemical Systems Ltd Apparatus and method for performing an assay
US7402279B2 (en) * 2002-10-31 2008-07-22 Agilent Technologies, Inc. Device with integrated microfluidic and electronic components
EP1567638A1 (en) * 2002-11-08 2005-08-31 Irm, Llc Apparatus and methods to process substrate surface features
US20040157336A1 (en) * 2002-11-14 2004-08-12 Affymetrix, Inc. Automated fluid control system and process
US20040101870A1 (en) * 2002-11-26 2004-05-27 Caubet Bruno S. Microvolume biochemical reaction chamber
TWI255971B (en) 2002-11-29 2006-06-01 Asml Netherlands Bv Lithographic apparatus and device manufacturing method
TWI245739B (en) * 2002-12-05 2005-12-21 Ibm Method and device for flowing a liquid on a surface
EP1608952B1 (en) * 2002-12-20 2016-08-10 Life Technologies Corporation Assay apparatus and method using microfluidic arrays
JP2006512092A (en) 2002-12-30 2006-04-13 ザ・リージェンツ・オブ・ジ・ユニバーシティ・オブ・カリフォルニア Method and apparatus for pathogen detection and analysis
JP3920223B2 (en) * 2003-01-07 2007-05-30 日本碍子株式会社 Reactive chip and target substance binding detection method using this chip
CN1249437C (en) * 2003-02-17 2006-04-05 中国科学院力学研究所 Method and apparatus for bio-molecular chip minute quantity sample application and reaction
WO2004083824A1 (en) * 2003-03-20 2004-09-30 Dakocytomation Denmark A/S System for establishing a sample cover on a substrate
US20060073074A1 (en) * 2004-10-06 2006-04-06 Lars Winther Enhanced sample processing system and methods of biological slide processing
US20040235147A1 (en) * 2003-05-21 2004-11-25 Affymetrix, Inc. System, method, and encased probe array product
US20040241660A1 (en) * 2003-05-30 2004-12-02 Applera Corporation Apparatus and method for hybridization
US7055695B2 (en) 2003-06-02 2006-06-06 Caliper Life Sciencee, Inc. Container providing a controlled hydrated environment
US20050079517A1 (en) * 2003-06-19 2005-04-14 Michael Goncharko Controlled evaporation, temperature control and packaging for optical inspection of biological samples
US8679853B2 (en) * 2003-06-20 2014-03-25 Roche Diagnostics Operations, Inc. Biosensor with laser-sealed capillary space and method of making
US20080257754A1 (en) * 2003-06-27 2008-10-23 Pugia Michael J Method and apparatus for entry of specimens into a microfluidic device
US20040265171A1 (en) * 2003-06-27 2004-12-30 Pugia Michael J. Method for uniform application of fluid into a reactive reagent area
US20060240573A1 (en) * 2003-07-29 2006-10-26 Lamdagen, Llc Optical system including nanostructures for biological or chemical sensing
JP4996248B2 (en) 2003-07-31 2012-08-08 ハンディーラブ インコーポレイテッド Processing of particle-containing samples
US7744817B2 (en) * 2003-08-11 2010-06-29 Sakura Finetek U.S.A., Inc. Manifold assembly
US9518899B2 (en) 2003-08-11 2016-12-13 Sakura Finetek U.S.A., Inc. Automated reagent dispensing system and method of operation
US7767152B2 (en) * 2003-08-11 2010-08-03 Sakura Finetek U.S.A., Inc. Reagent container and slide reaction retaining tray, and method of operation
DE10340473B4 (en) * 2003-09-03 2007-08-23 Eppendorf Ag Cover of a hybridization chamber
US9597644B2 (en) 2003-09-05 2017-03-21 Stokes Bio Limited Methods for culturing and analyzing cells
EP1663497B2 (en) 2003-09-05 2020-03-25 Stokes Bio Limited A microfluidic analysis system
US7407630B2 (en) * 2003-09-19 2008-08-05 Applera Corporation High density plate filler
US20050084866A1 (en) * 2003-10-15 2005-04-21 Caren Michael P. Methods and apparatus for sample mixing
EP1525916A1 (en) * 2003-10-23 2005-04-27 F. Hoffmann-La Roche Ag Flow triggering device
US20050095602A1 (en) * 2003-11-04 2005-05-05 West Jason A. Microfluidic integrated microarrays for biological detection
US7238521B2 (en) * 2003-11-24 2007-07-03 Biocept, Inc. Microarray hybridization device having bubble-fracturing elements
AU2004312893B2 (en) * 2003-12-31 2010-06-17 President And Fellows Of Harvard College Assay device and method
ES2614425T3 (en) * 2004-01-08 2017-05-31 Dako Denmark A/S Apparatus and methods for processing biological samples and a deposit for them
SG152261A1 (en) 2004-01-25 2009-05-29 Fluidigm Corp Crystal forming devices and systems and methods for making and using the same
JP3860174B2 (en) * 2004-03-01 2006-12-20 倉敷紡績株式会社 Simultaneous multi-sample hybridization method
CA2559171A1 (en) 2004-03-12 2005-09-29 Biotrove, Inc. Nanoliter array loading
US8444934B2 (en) 2004-03-26 2013-05-21 Universite Laval Removable microfluidic flow cell
US20050214930A1 (en) * 2004-03-26 2005-09-29 Bynum Magdalena A Apparatus and method for preventing cross contamination when dissassembling an array
FR2868431B1 (en) * 2004-03-31 2006-05-26 Inodiag Sa INCUBATION DEVICE FOR BLADES OF SEROLOGY AND HISTOLOGY
EP1740984A4 (en) * 2004-04-02 2011-04-06 Eksigent Technologies Llc Microfluidic connections
US20050244951A1 (en) * 2004-04-30 2005-11-03 Gordon Gary B Array hybridization chamber with disassembly feature and method
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
AU2005241080B2 (en) * 2004-05-03 2011-08-11 Handylab, Inc. Processing polynucleotide-containing samples
DE102004022263A1 (en) * 2004-05-06 2005-12-15 Clondiag Chip Technologies Gmbh Apparatus and method for detecting molecular interactions
DE102005052713A1 (en) * 2005-11-04 2007-05-16 Clondiag Chip Tech Gmbh Apparatus and method for detecting molecular interactions
US7799553B2 (en) * 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
JP2008513022A (en) * 2004-09-15 2008-05-01 マイクロチップ バイオテクノロジーズ， インコーポレイテッド Microfluidic device
KR100668304B1 (en) * 2004-09-16 2007-01-12 삼성전자주식회사 A device for the injection of PCR solution into a PCR channel and a PCR chip unit comprising the device
KR100601966B1 (en) * 2004-10-07 2006-07-18 삼성전자주식회사 A microchip unit and a method for conducting a biochemical reaction by using the microchip unit
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2006054238A2 (en) * 2004-11-16 2006-05-26 Koninklijke Philips Electronics N. V. Microfluidic device
JP2006153785A (en) * 2004-12-01 2006-06-15 Hitachi Ltd Solution stirring device and analyzing system
US9260693B2 (en) 2004-12-03 2016-02-16 Cytonome/St, Llc Actuation of parallel microfluidic arrays
KR100612879B1 (en) 2004-12-06 2006-08-14 삼성전자주식회사 Hybridization device using the control of pump and valves in closed system
US20060166223A1 (en) * 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
CA2535317C (en) * 2005-02-11 2010-04-13 Sakura Finetek U.S.A., Inc. Reagent container and slide reaction and retaining tray, and method of operation
US20090220948A1 (en) * 2005-03-16 2009-09-03 Attogenix Biosystems Pte Ltd. Methods and Device for Transmitting, Enclosing and Analysing Fluid Samples
JPWO2006098370A1 (en) * 2005-03-16 2008-08-28 日本電気株式会社 DELAY CIRCUIT, MICROCHIP HAVING ADJUSTING MECHANISM FOR EFFECTIVE PASSING TIME OF PATH, AND METHOD FOR MANUFACTURING THE SAME
US20090111197A1 (en) * 2005-03-29 2009-04-30 Inverness Medical Switzerland Gmbh Hybrid device
JP2008537148A (en) 2005-04-21 2008-09-11 セレラス ダイアグノスティクス， インコーポレイテッド Wicking cassette method and apparatus for automated rapid immunohistochemistry
GB0508983D0 (en) * 2005-05-03 2005-06-08 Oxford Gene Tech Ip Ltd Cell analyser
US8206974B2 (en) * 2005-05-19 2012-06-26 Netbio, Inc. Ruggedized apparatus for analysis of nucleic acid and proteins
DE102005026528B4 (en) 2005-06-08 2019-08-22 Infineon Technologies Ag Semiconductor device having at least one media channel and method for producing semiconductor devices each having at least one media channel
US20060280029A1 (en) * 2005-06-13 2006-12-14 President And Fellows Of Harvard College Microfluidic mixer
TWI253435B (en) * 2005-06-13 2006-04-21 Univ Chung Yuan Christian Loop micro fluid system
CN101203296A (en) 2005-06-23 2008-06-18 皇家飞利浦电子股份有限公司 Device for mixing a liquid medium
JP4673149B2 (en) * 2005-06-29 2011-04-20 ローム株式会社 Method of using microchip, microchannel, and microchip
US8288151B2 (en) * 2005-06-29 2012-10-16 Canon Kabushiki Kaisha Biochemical reaction cassette
WO2007027928A1 (en) * 2005-09-02 2007-03-08 California Institute Of Technology Method and apparatus for the mechanical actuation of valves in fluidic devices
EP1940543B1 (en) 2005-09-29 2012-03-07 Siemens Medical Solutions USA, Inc. Microfluidic chip capable of synthesizing radioactively labeled molecules on a scale suitable for human imaging with positron emission tomography
EP1945815A4 (en) * 2005-10-11 2009-02-18 Handylab Inc Polynucleotide sample preparation device
US7506952B2 (en) * 2005-10-11 2009-03-24 Silverbrook Research Pty Ltd Method of removing particulates from a printhead using film transfer
US7758174B2 (en) * 2005-10-11 2010-07-20 Silverbrook Research Pty Ltd Ink supply system comprising air compressor and in-line valve
WO2007044856A1 (en) * 2005-10-11 2007-04-19 The Johns Hopkins University Device and method for high-throughput stimulation, immunostaining, and visualization of single cells
US7370936B2 (en) * 2005-10-11 2008-05-13 Silverbrook Research Pty Ltd Method of maintaining a printhead using film transport of ink
WO2007044888A2 (en) * 2005-10-11 2007-04-19 The Johns Hopkins Univerisity Microfluidic device and method for high-throughput cellular gradient and dose response studies
EP1946830B1 (en) * 2005-11-07 2019-04-03 Konica Minolta Medical & Graphic, Inc. Microreactor
JP4556849B2 (en) * 2005-11-14 2010-10-06 株式会社日立プラントテクノロジー Microreactor system
KR20070052958A (en) * 2005-11-18 2007-05-23 주식회사 엘지생명과학 Plastic chip for pcr having on-chip polymer valve
CN102327738A (en) * 2005-11-22 2012-01-25 迈克罗拉布诊断有限公司 Liquid processing structure, flow control device and plug member assembling and method
US9056291B2 (en) 2005-11-30 2015-06-16 Micronics, Inc. Microfluidic reactor system
US7763453B2 (en) * 2005-11-30 2010-07-27 Micronics, Inc. Microfluidic mixing and analytic apparatus
CN1987480A (en) * 2005-12-23 2007-06-27 博奥生物有限公司 Sample adding mouth for adding fluid sample to hydrophilic micro fine pipeline by transfer pipet sucking head
US7749365B2 (en) 2006-02-01 2010-07-06 IntegenX, Inc. Optimized sample injection structures in microfluidic separations
US8616048B2 (en) * 2006-02-02 2013-12-31 E I Spectra, LLC Reusable thin film particle sensor
US9452429B2 (en) 2006-02-02 2016-09-27 E. I. Spectra, Llc Method for mutiplexed microfluidic bead-based immunoassay
US8171778B2 (en) * 2006-05-05 2012-05-08 E I Spectra, LLC Thin film particle sensor
US7520164B1 (en) * 2006-05-05 2009-04-21 E.I. Spectra, Llc Thin film particle sensor
US9293311B1 (en) 2006-02-02 2016-03-22 E. I. Spectra, Llc Microfluidic interrogation device
US20110189714A1 (en) * 2010-02-03 2011-08-04 Ayliffe Harold E Microfluidic cell sorter and method
WO2008030631A2 (en) 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
US7862000B2 (en) 2006-02-03 2011-01-04 California Institute Of Technology Microfluidic method and structure with an elastomeric gas-permeable gasket
WO2007091228A1 (en) 2006-02-07 2007-08-16 Stokes Bio Limited A liquid bridge and system
US20100200021A1 (en) * 2006-02-17 2010-08-12 Nils Adey Slide Conditioning Systems and Methods
DE102006009424A1 (en) * 2006-02-24 2007-09-06 Universität Rostock Electrohydrodynamic micropump and its use
US7766033B2 (en) * 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) * 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
DK2001990T3 (en) * 2006-03-24 2016-10-03 Handylab Inc Integrated microfluidic sample processing system and method for its use
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US20090181864A1 (en) * 2006-03-31 2009-07-16 Nam Trung Nguyen Active control for droplet-based microfluidics
EP2047910B1 (en) 2006-05-11 2012-01-11 Raindance Technologies, Inc. Microfluidic device and method
US9562837B2 (en) * 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US8459509B2 (en) 2006-05-25 2013-06-11 Sakura Finetek U.S.A., Inc. Fluid dispensing apparatus
US20070280857A1 (en) * 2006-06-02 2007-12-06 Applera Corporation Devices and Methods for Positioning Dried Reagent In Microfluidic Devices
AU2007265628B2 (en) * 2006-06-23 2012-12-06 Perkinelmer Health Sciences, Inc. Methods and devices for microfluidic point-of-care immunoassays
EP1870687B1 (en) * 2006-06-23 2012-09-12 STMicroelectronics Srl Assembly of an integrated device enabling a facilitated fluidic connection to regions of the device
WO2008004550A1 (en) * 2006-07-05 2008-01-10 Hitachi High-Technologies Corporation Liquid analyzer
KR100790881B1 (en) * 2006-07-06 2008-01-02 삼성전자주식회사 Micro-fluid reaction chip, and method for manufacturing the same
US20080031089A1 (en) * 2006-08-04 2008-02-07 Zhenghua Ji Low-volume mixing of sample
US20080032311A1 (en) * 2006-08-04 2008-02-07 Zhenghua Ji Low volume mixing of sample
US10753927B2 (en) 2006-09-22 2020-08-25 ALERE TECHNOLOGIES GmbH Methods for detecting an analyte
US20080131327A1 (en) * 2006-09-28 2008-06-05 California Institute Of Technology System and method for interfacing with a microfluidic chip
US7718420B2 (en) * 2006-10-10 2010-05-18 Postech Academy-Industry Foundation Microfluidic biochip for blood typing based on agglutination reaction
WO2008052138A2 (en) * 2006-10-25 2008-05-02 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated dna analysis system using same
JP2008122179A (en) * 2006-11-10 2008-05-29 Konica Minolta Medical & Graphic Inc Micro-integrated analysis chip and micro-integrated analysis system
JP2008122234A (en) * 2006-11-13 2008-05-29 Konica Minolta Medical & Graphic Inc Micro-integrated analysis chip and micro-integrated analysis system
JP2008122233A (en) * 2006-11-13 2008-05-29 Konica Minolta Medical & Graphic Inc Micro-integrated analysis chip and micro-integrated analysis system
US8709787B2 (en) * 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
EA201201363A1 (en) 2006-11-21 2013-08-30 Медимейт Холдинг Б.В. ION SENSOR FOR FLUID ENVIRONMENT AND METHOD FOR ITS MANUFACTURE
JP5137551B2 (en) * 2006-12-28 2013-02-06 キヤノン株式会社 Biochemical reaction cassette
US20100248975A1 (en) * 2006-12-29 2010-09-30 Gunjan Tiwari Fluorogenic peptide substrate arrays for highly multiplexed, real-time monitoring of kinase activities
US8426152B2 (en) * 2007-01-03 2013-04-23 Lamdagen Corporation Enzymatic assay for LSPR
WO2008091694A2 (en) * 2007-01-23 2008-07-31 Siemens Medical Solutions Usa, Inc. Fully-automated microfluidic system for the synthesis of radiolabeled biomarkers for positron emission tomography
KR20100028526A (en) * 2007-02-05 2010-03-12 마이크로칩 바이오테크놀로지스, 인크. Microfluidic and nanofluidic devices, systems, and applications
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US7863035B2 (en) * 2007-02-15 2011-01-04 Osmetech Technology Inc. Fluidics devices
US8071035B2 (en) 2007-04-12 2011-12-06 Siemens Medical Solutions Usa, Inc. Microfluidic radiosynthesis system for positron emission tomography biomarkers
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
CA2684095C (en) * 2007-05-03 2016-12-13 Clondiag Gmbh Devices and methods for detecting analytes
KR100870982B1 (en) 2007-05-03 2008-12-01 주식회사 바이오트론 A multilayer Bio-reactor
WO2008143959A1 (en) * 2007-05-15 2008-11-27 Wako Pure Chemical Industries, Ltd. Pressure manifold to equalize pressure in integration pcr-ce microfluidic devices
BRPI0721675A2 (en) * 2007-05-18 2013-01-22 Medimate Holding B V measuring sample handling device, socket connectable to a measuring measuring device, measuring measuring device, method for evaluating at least one parameter of a liquid sample, method for mounting a measuring sample handling device
US20080294361A1 (en) * 2007-05-24 2008-11-27 Popp Shane M Intelligent execution system for the monitoring and execution of vaccine manufacturing
WO2009002447A1 (en) 2007-06-21 2008-12-31 Gen-Probe Incorporated Instrument and receptacles for use in performing processes
US20090017528A1 (en) * 2007-07-12 2009-01-15 Pei-Tai Chen Nucleic Acid Hybridization Device
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US9618139B2 (en) * 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8324372B2 (en) 2007-07-13 2012-12-04 Handylab, Inc. Polynucleotide capture materials, and methods of using same
WO2009015296A1 (en) * 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
US7993608B2 (en) * 2007-08-07 2011-08-09 Massachusetts Institute Of Technology Fluid injection port
US9440231B2 (en) 2007-08-14 2016-09-13 Fluidigm Corporation Polymer microfluidic biochip fabrication
JP2010536565A (en) * 2007-08-23 2010-12-02 シンベニオ・バイオシステムズ・インコーポレーテッド Magnetic sorting system for traps for target species
US7695976B2 (en) * 2007-08-29 2010-04-13 Plexera Bioscience, Llc Method for uniform analyte fluid delivery to microarrays
US20090139311A1 (en) 2007-10-05 2009-06-04 Applied Biosystems Inc. Biological Analysis Systems, Devices, and Methods
DE202007016855U1 (en) * 2007-11-30 2008-02-14 DKFZ Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Incubation tray for protein array incubations
US20090253181A1 (en) * 2008-01-22 2009-10-08 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
WO2009117611A2 (en) * 2008-03-19 2009-09-24 Cynvenio Biosystems, Llc Trapping magnetic cell sorting system
CN101980769B (en) * 2008-03-28 2013-06-12 爱科来株式会社 Fluid agitation method, fluid agitation system, and cartridge
WO2009137244A1 (en) * 2008-04-15 2009-11-12 Charles River Laboratories, Inc. Cartridge and method for sample analysis
EP2271919A1 (en) * 2008-04-16 2011-01-12 Cynvenio Biosystems, Inc. Magnetic separation system with pre and post processing modules
US8680025B2 (en) * 2008-05-09 2014-03-25 Akonni Biosystems, Inc. Microarray system
US8680026B2 (en) * 2008-05-09 2014-03-25 Akonni Biosystems, Inc. Flow cell device
CA2723707C (en) * 2008-05-09 2016-08-23 Akonni Biosystems Microarray system
US8975039B2 (en) * 2008-06-09 2015-03-10 SYFR, Inc. Automatic sample staining method
US20110190153A1 (en) * 2008-06-09 2011-08-04 Nils Adey System and method for hybridization slide processing
US20100167943A1 (en) * 2008-06-09 2010-07-01 Nils Adey System and Method for Hybridization Slide Processing
EP2143491A1 (en) * 2008-07-10 2010-01-13 Carpegen GmbH Device for analysing a chemical or biological sample
US20100009351A1 (en) * 2008-07-11 2010-01-14 Handylab, Inc. Polynucleotide Capture Materials, and Method of Using Same
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
CN101368206B (en) * 2008-07-16 2012-08-22 深圳华因康基因科技有限公司 Sequencing reaction small chamber, gene sequencing reaction bench and gene sequencing device
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
EP2318510B1 (en) 2008-08-01 2018-09-19 Kirkstall Limited Improved bioreactor chamber
GB0815472D0 (en) * 2008-08-22 2008-10-01 Iti Scotland Ltd Microfluidic system
WO2010025282A2 (en) 2008-08-29 2010-03-04 Infusion Innnovations, Inc Check valve-less fluid-transfer collection assembly and method of using the same
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
WO2010042539A1 (en) * 2008-10-10 2010-04-15 Cytyc Corporation Microfluidic apparatus and method for preparing cytological specimens
US20100093098A1 (en) * 2008-10-14 2010-04-15 Siemens Medical Solutions Nonflow-through appratus and mehod using enhanced flow mechanisms
EP2350673B1 (en) * 2008-10-24 2022-05-11 Leica Biosystems Richmond, Inc. Modular system for performing laboratory protocols and associated methods
CH699853A1 (en) * 2008-11-13 2010-05-14 Tecan Trading Ag Meter and method for determining provided by a laboratory fluid system parameters.
US8507231B2 (en) * 2008-11-25 2013-08-13 Syngenta Participations Ag High throughput screening platform for high ethanol production
WO2010077322A1 (en) 2008-12-31 2010-07-08 Microchip Biotechnologies, Inc. Instrument with microfluidic chip
FR2941385B1 (en) * 2009-01-23 2011-04-01 Millipore Corp METHOD FOR PROVIDING A CIRCUIT FOR BIOLOGICAL LIQUID AND CIRCUIT OBTAINED
FR2941531B1 (en) * 2009-01-26 2019-07-05 Phuong Lan TRAN MICROFLUIDIC DEVICE, SYSTEM AND METHOD FOR IMPLEMENTING THE SAME
WO2010119396A1 (en) * 2009-04-15 2010-10-21 Koninklijke Philips Electronics N.V. Cartridge for an amplification process
CN102413913A (en) 2009-04-23 2012-04-11 皇家飞利浦电子股份有限公司 Mixer with zero dead volume and method for mixing
WO2010137543A1 (en) * 2009-05-27 2010-12-02 株式会社日立ハイテクノロジーズ Nucleic acid analysis device, nucleic acid analysis apparatus, and nucleic acid analysis method
US20100300563A1 (en) * 2009-05-27 2010-12-02 John Ramunas Modular device and method for moving fluids to and from a sample delivery element
WO2010141326A1 (en) 2009-06-02 2010-12-09 Integenx Inc. Fluidic devices with diaphragm valves
EP3586945A3 (en) 2009-06-05 2020-03-04 IntegenX Inc. Universal sample preparation system and use in an integrated analysis system
WO2010144745A2 (en) * 2009-06-10 2010-12-16 Cynvenio Biosystems, Inc. Sheath flow devices and methods
DE102009033008A1 (en) 2009-07-02 2011-01-05 Dst Diagnostische Systeme & Technologien Gmbh New PoC test system and procedures
EP2451575A2 (en) * 2009-07-07 2012-05-16 Boehringer Ingelheim Microparts GmbH Plasma separation reservoir
US8758704B2 (en) * 2009-08-11 2014-06-24 Baril Corporation Microfluidic diagnostic device
WO2011026136A1 (en) 2009-08-31 2011-03-03 Life Technologies Corporation Low-volume sequencing system and method of use
SG169918A1 (en) * 2009-10-02 2011-04-29 Fluidigm Corp Microfluidic devices with removable cover and methods of fabrication and application
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
KR101851117B1 (en) 2010-01-29 2018-04-23 마이크로닉스 인코포레이티드. Sample-to-answer microfluidic cartridge
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP2534267B1 (en) 2010-02-12 2018-04-11 Raindance Technologies, Inc. Digital analyte analysis
EP2366993A1 (en) 2010-03-08 2011-09-21 Nxp B.V. A sensor and a method of assembling a sensor
JP5497587B2 (en) * 2010-03-23 2014-05-21 株式会社日立ハイテクノロジーズ Microchannel chip and microarray chip
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
ITTO20100068U1 (en) * 2010-04-20 2011-10-21 Eltek Spa MICROFLUID AND / OR EQUIPMENT DEVICES FOR MICROFLUID DEVICES
DE102010028012A1 (en) * 2010-04-21 2011-10-27 Qiagen Gmbh Liquid control for micro flow system
US10451532B2 (en) 2010-04-26 2019-10-22 Rheosense, Inc. Portable viscometer
WO2011139888A2 (en) 2010-04-29 2011-11-10 Statspin, Inc. Analytical system for performing laboratory protocols and associated methods
US9707563B2 (en) * 2010-05-04 2017-07-18 Agency For Science, Technology And Research Reagent fluid dispensing device, and method of dispensing a reagent fluid
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
US10216698B2 (en) 2010-06-07 2019-02-26 Commissariat à l 'Energie Atomique et aux Energies Alternatives Analysis device including a MEMS and/or NEMS network
KR101248189B1 (en) * 2010-06-10 2013-03-27 주식회사 제이오텍 Test apparatus using of gene chip
CH703278B1 (en) 2010-06-15 2016-11-15 Csem Centre Suisse D'electronique Et De Microtechnique Sa - Rech Et Développement Device and platform for multiplex analysis.
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
US9121058B2 (en) 2010-08-20 2015-09-01 Integenx Inc. Linear valve arrays
KR101044363B1 (en) * 2010-08-23 2011-06-29 주식회사 월드퍼니처 Environment-friendly loess panel with improved mechanical strength
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
EP2652659B1 (en) 2010-12-14 2020-04-15 Life Technologies Corporation Systems and methods for run-time sequencing run quality monitoring
JP5616773B2 (en) * 2010-12-21 2014-10-29 株式会社日立ハイテクノロジーズ Reaction device for nucleic acid analysis and nucleic acid analyzer
US9139316B2 (en) 2010-12-29 2015-09-22 Cardinal Health 414, Llc Closed vial fill system for aseptic dispensing
US8951781B2 (en) * 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
CN110220876B (en) * 2011-01-10 2022-04-26 伊鲁米那股份有限公司 Method for imaging a sample for biological or chemical analysis
CN102175589B (en) * 2011-01-20 2013-04-17 重庆天海医疗设备有限公司 Flow type counting chamber and urine sample sampling method thereof
US8752732B2 (en) 2011-02-01 2014-06-17 Sakura Finetek U.S.A., Inc. Fluid dispensing system
EP2673614B1 (en) 2011-02-11 2018-08-01 Raindance Technologies, Inc. Method for forming mixed droplets
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US8709353B2 (en) * 2011-03-24 2014-04-29 Boehringer Ingelheim Microparts Gmbh Device and method for producing a fluidic connection between cavities
BR112013026451B1 (en) 2011-04-15 2021-02-09 Becton, Dickinson And Company system and method to perform molecular diagnostic tests on several samples in parallel and simultaneously amplification in real time in plurality of amplification reaction chambers
WO2012144794A2 (en) * 2011-04-19 2012-10-26 주식회사 바이오포커스 Method for manufacturing a microvalve device mounted on a lab-on-a-chip, and microvalve device manufactured by same
JP6084370B2 (en) * 2011-05-10 2017-02-22 国立大学法人 東京大学 Automatic reaction equipment for histochemistry
DE202012013668U1 (en) 2011-06-02 2019-04-18 Raindance Technologies, Inc. enzyme quantification
KR101197208B1 (en) * 2011-06-29 2012-11-02 한국과학기술원 Micro pump and driving method thereof
US8357293B1 (en) * 2011-07-11 2013-01-22 Gerhardt Van Drie Biological waste digester
US9417332B2 (en) 2011-07-15 2016-08-16 Cardinal Health 414, Llc Radiopharmaceutical CZT sensor and apparatus
WO2013012822A1 (en) 2011-07-15 2013-01-24 Cardinal Health 414, Llc Systems, methods, and devices for producing, manufacturing, and control of radiopharmaceuticals
US20130020727A1 (en) 2011-07-15 2013-01-24 Cardinal Health 414, Llc. Modular cassette synthesis unit
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
DK2753428T3 (en) * 2011-09-09 2019-01-21 Ventana Med Syst Inc Slide transfer device
US8932543B2 (en) 2011-09-21 2015-01-13 Sakura Finetek U.S.A., Inc. Automated staining system and reaction chamber
US8580568B2 (en) 2011-09-21 2013-11-12 Sakura Finetek U.S.A., Inc. Traceability for automated staining system
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
WO2013049706A1 (en) 2011-09-30 2013-04-04 Becton, Dickinson And Company Unitized reagent strip
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
WO2013073726A1 (en) * 2011-11-17 2013-05-23 인제대학교 산학협력단 Device for separating single cells and fixing and maintaining position of single cells
CN104136123B (en) 2012-01-09 2017-03-01 精密公司 Microfluidic reactor system
WO2013111025A1 (en) * 2012-01-24 2013-08-01 Koninklijke Philips N.V. Flow through device for staining and/or analyzing a biological sample
CN104204812B (en) 2012-02-03 2018-01-05 贝克顿·迪金森公司 The external file that compatibility determines between distributing and test for molecule diagnostic test
CA2902127C (en) * 2012-02-27 2021-08-10 Ecole Polytechnique Federale De Lausanne (Epfl) Sample processing device with detachable slide
US9909962B2 (en) 2012-03-09 2018-03-06 Leica Biosystems Richmond, Inc. Device and method for controlling the temperature in a moving fluid in a laboratory sample processing system
CN104411406B (en) 2012-03-16 2017-05-31 统计诊断与创新有限公司 Testing cassete with integrated delivery module
US8804105B2 (en) 2012-03-27 2014-08-12 E. I. Spectra, Llc Combined optical imaging and electrical detection to characterize particles carried in a fluid
JP2013205282A (en) * 2012-03-29 2013-10-07 Brother Ind Ltd Inspection object holder, inspection device, and inspection method
US9797006B2 (en) * 2012-04-10 2017-10-24 Keck Graduate Institute Of Applied Life Sciences System and cartridge for efficient nucleic acid testing
US9080941B2 (en) 2012-04-27 2015-07-14 General Electric Company Microfluidic flow cell assemblies for imaging and method of use
US9150907B2 (en) 2012-04-27 2015-10-06 General Electric Company Microfluidic flow cell assemblies and method of use
US8900529B2 (en) 2012-04-27 2014-12-02 General Electric Company Microfluidic chamber device and fabrication
JP5692164B2 (en) * 2012-05-22 2015-04-01 ウシオ電機株式会社 Reagent supply method to microchip and reagent supply apparatus to microchip
KR101337136B1 (en) * 2012-05-30 2013-12-05 충북대학교 산학협력단 Droplet-based microfluidic system integrated with diaphragm feeder
JP6396911B2 (en) 2012-10-15 2018-09-26 ナノセレクト バイオメディカル， インコーポレイテッド System, apparatus and method for sorting particles
US20140322706A1 (en) * 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
EP2912432B1 (en) 2012-10-24 2018-07-04 Genmark Diagnostics Inc. Integrated multiplex target analysis
WO2014066947A1 (en) * 2012-11-01 2014-05-08 Leica Biosystems Melbourne Pty Ltd A fluid transport system
EP2932266A4 (en) * 2012-12-17 2016-11-30 Leukodx Ltd Systems and methods for determining a chemical state
JP6498125B2 (en) 2012-12-21 2019-04-10 マイクロニクス， インコーポレイテッド Fluid circuit and associated manufacturing method
EP2934730B1 (en) * 2012-12-21 2019-09-25 Leica Biosystems Melbourne Pty Ltd Method of producing a reagent on-board an instrument
JP2016509206A (en) 2012-12-21 2016-03-24 マイクロニクス， インコーポレイテッド Portable fluorescence detection system and microassay cartridge
US10518262B2 (en) 2012-12-21 2019-12-31 Perkinelmer Health Sciences, Inc. Low elasticity films for microfluidic use
US9687847B1 (en) * 2013-02-13 2017-06-27 University Of South Florida Microfluidic platforms for optical biosensing
US9636673B2 (en) * 2013-03-01 2017-05-02 Micronit Microfluidics B.V. Method for manufacturing microfluidic chips, device for functionalizing microfluidic chips, microfluidic chip and device for holding a microfluidic chip
US10168341B2 (en) * 2013-03-08 2019-01-01 Emory University Devices for determining cell force properties and methods of manufacturing the devices
EP3520895A1 (en) 2013-03-15 2019-08-07 Genmark Diagnostics Inc. Fluid container with cantilevered lance
US10793820B2 (en) * 2013-04-30 2020-10-06 Lawrence Livermore National Security, Llc Miniaturized, automated in-vitro tissue bioreactor
EP2799137A1 (en) * 2013-04-30 2014-11-05 Koninklijke Philips N.V. Fluidic system for processing a sample fluid
WO2014182844A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
WO2014182831A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
EP2994543B1 (en) 2013-05-07 2018-08-15 Micronics, Inc. Device for preparation and analysis of nucleic acids
EP3051293B1 (en) * 2013-09-25 2021-11-03 The University of Tokyo Solution mixer, fluid device, and solution mixing method
US9604214B2 (en) * 2013-10-01 2017-03-28 Owl biomedical, Inc. Cell sorting system using microfabricated components
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9453996B2 (en) 2013-10-23 2016-09-27 Tokitae Llc Devices and methods for staining and microscopy
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
US9463461B2 (en) * 2013-10-25 2016-10-11 The Johns Hopkins University Self-contained cartridge and methods for integrated biochemical assay at the point-of-care
GB2520063B (en) * 2013-11-08 2018-01-31 Intelligent Fingerprinting Ltd Skin-print fluorescence analysis method and apparatus
CN114471756B (en) 2013-11-18 2024-04-16 尹特根埃克斯有限公司 Cartridge and instrument for sample analysis
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
EP3155399B1 (en) 2014-04-11 2020-12-02 Rheosense Inc. Viscometer and methods for using the same
WO2015179098A1 (en) 2014-05-21 2015-11-26 Integenx Inc. Fluidic cartridge with valve mechanism
WO2015181367A1 (en) * 2014-05-29 2015-12-03 Ge Healthcare Bio-Sciences Corp Improvements in and relating to cell culture microscopy slides
US10124333B2 (en) 2014-06-10 2018-11-13 Reolab Inc. Discrete elements for 3D microfluidics
WO2015189390A1 (en) 2014-06-13 2015-12-17 Q-Linea Ab Method for detecting and characterising a microorganism
EP3157680B1 (en) 2014-06-19 2019-05-29 Akonni Biosystems, Inc. Molecular analysis system and use thereof
EP3171151A4 (en) * 2014-07-18 2018-02-28 Shimadzu Corporation Device for constant-volume collection using centrifugation or for further storage
US10632462B2 (en) * 2014-09-15 2020-04-28 The Trustees Of The University Of Pennsylvania Apparatus and methods for manufacturing a microfluidic device
CN113092563A (en) 2014-10-22 2021-07-09 尹特根埃克斯有限公司 Systems and methods for sample preparation, processing, and analysis
JP6812797B2 (en) * 2014-11-04 2021-01-13 凸版印刷株式会社 Nucleic acid introduction method, nucleic acid detection method, biological component analysis method, array device for quantifying biological components, and biological component analysis kit
US10005080B2 (en) 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
US9598722B2 (en) 2014-11-11 2017-03-21 Genmark Diagnostics, Inc. Cartridge for performing assays in a closed sample preparation and reaction system
US9599629B2 (en) * 2014-11-14 2017-03-21 Cytoskeleton, Inc. Modular fluid dispensing devices
WO2016122588A1 (en) * 2015-01-30 2016-08-04 Hewlett-Packard Development Company, L.P. Diagnostic device
WO2016153000A1 (en) * 2015-03-24 2016-09-29 国立大学法人東京大学 Fluid device, system and method
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
BR112017025587B1 (en) * 2015-05-29 2022-09-27 Illumina, Inc SAMPLE CARRIER AND ASSAY SYSTEM TO CONDUCT DESIGNATED REACTIONS
JP6640998B2 (en) * 2015-06-05 2020-02-05 ミメタス ビー．ブイ． Micro fluid plate
TWI581862B (en) * 2015-06-16 2017-05-11 亞諾法生技股份有限公司 Holding carrier for a microfluidic device
WO2016209734A1 (en) * 2015-06-22 2016-12-29 Fluxergy, Llc Device for analyzing a fluid sample and use of test card with same
WO2017015123A1 (en) * 2015-07-17 2017-01-26 Boyd Lawrence M Apparatus and method for sorting of cells
EP3325931B1 (en) * 2015-07-20 2020-10-28 Haemonetics Corporation System and method for measuring pressure of fluid flow
CN105154323B (en) * 2015-08-14 2017-08-29 深圳市瀚海基因生物科技有限公司 A kind of single-molecule sequencing chip
CN114540192A (en) 2015-08-26 2022-05-27 仿真股份有限公司 Fill manifold assembly
WO2017040733A1 (en) * 2015-09-01 2017-03-09 President And Fellows Of Harvard College Balloon actuation for laminated devices
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
US9958362B1 (en) * 2015-10-02 2018-05-01 The Florida State University Research Foundation, Inc. Microscope sample preparation device
GB201518993D0 (en) 2015-10-27 2015-12-09 Randox Lab Ltd Improvements relating to analysis of biochips
WO2017081115A1 (en) * 2015-11-13 2017-05-18 Ventana Medical Systems, Inc. Closed-system passive mixing flow cell system for tissue slide staining
EP3413056B1 (en) * 2016-02-02 2019-12-18 Konica Minolta, Inc. Liquid delivery method, and detection system using said method
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
US10288556B2 (en) 2016-04-21 2019-05-14 Instrumentation Laboratory Company Optical flow cell apparatus and method for reducing deflection of sample chamber
KR20240023193A (en) 2016-07-12 2024-02-20 에뮬레이트, 인크. Method of increasing the gas carrying capacity of a fluid and removing any bubbles present in the fluid in a microfluidic device
JP7114602B2 (en) * 2016-09-30 2022-08-08 テル－アレイ ダイアグノスティックス インコーポレイテッド microfluidic device
WO2018151724A1 (en) * 2017-02-15 2018-08-23 Hewlett-Packard Development Company, L.P. Microfluidic network
WO2018175411A1 (en) * 2017-03-20 2018-09-27 Nanocellect Biomedical, Inc. Systems, apparatuses, and methods for cell sorting and flow cytometry
GB2562081B (en) * 2017-05-04 2020-07-15 Imrali Ahmet Slide cleaner
KR101885087B1 (en) * 2017-06-12 2018-08-03 티엔에스(주) Chip for molecular diagnosis based on lap-on-a-chip
CN107225006B (en) * 2017-07-03 2018-04-03 南京岚煜生物科技有限公司 More flux micro-fluidic chips based on the flowing of active control liquid
CN109283135B (en) * 2017-07-21 2021-06-08 合肥欣奕华智能机器有限公司 Display panel detection device
US11022603B2 (en) 2017-08-30 2021-06-01 The Charles Stark Draper Laboratory, Inc. Microfluidic tissue biopsy and immune response drug evaluation devices and systems
WO2019075321A1 (en) * 2017-10-13 2019-04-18 The Charles Stark Draper Laboratory, Inc. Miniaturized dna microarray for small-volume sample processing
WO2019107231A1 (en) 2017-11-29 2019-06-06 積水化学工業株式会社 Micro-fluid chip
JP7111110B2 (en) * 2017-12-11 2022-08-02 株式会社ニコン Fluid device
JP6992824B2 (en) * 2018-01-29 2022-01-13 株式会社ニコン Fluid devices and systems
WO2019146102A1 (en) * 2018-01-29 2019-08-01 株式会社ニコン Fluid device and use thereof
WO2019168970A1 (en) * 2018-02-28 2019-09-06 Reolab Modular microchannel systems
EP3788381A4 (en) * 2018-04-30 2022-05-04 The Johns Hopkins University Disposable reagent scaffold for biochemical process integration
CN108854640A (en) * 2018-07-02 2018-11-23 苏州晨霞美合智能科技有限公司 A kind of new material processing raw material mixing equipment
EP3824265B1 (en) * 2018-07-16 2024-02-14 Ventana Medical Systems, Inc. Automated staining system
US20210308680A1 (en) * 2018-09-24 2021-10-07 University Of Manitoba A Passive Mixing Microfluidic Urinary Albumin Chip (UAL-Chip) for Chronic Kidney Disease
BR112020026480A2 (en) 2018-11-16 2021-05-18 Illumina, Inc. Laminated fluidic circuit apparatus and method for a fluid cartridge
US20200316593A1 (en) * 2018-12-06 2020-10-08 xCella Biosciences, Inc. Lateral loading of microcapillary arrays
US11161109B2 (en) * 2019-09-19 2021-11-02 Invidx Corp. Point-of-care testing cartridge with sliding cap
EP3798640A1 (en) * 2019-09-27 2021-03-31 Lunaphore Technologies SA Biological sample processing system and microfluidic cartridge therefor
CN111617812B (en) * 2019-10-17 2021-12-03 北京京东方健康科技有限公司 Microfluidic substrate, fluid driving method thereof and microfluidic device
WO2021116880A1 (en) * 2019-12-10 2021-06-17 Illumina Singapore Pte. Ltd. Actuation systems and methods for use with flow cells
CN113275045A (en) * 2020-02-20 2021-08-20 北京京东方健康科技有限公司 Detection chip, operation method thereof and detection system
US20230123901A1 (en) * 2020-03-26 2023-04-20 Siemens Healthcare Diagnostics Inc. Random access automated molecular testing system
KR102470163B1 (en) * 2020-12-24 2022-11-23 주식회사 바이오티엔에스 Lid heater assembly of pcr apparatus
JP2022159961A (en) * 2021-04-05 2022-10-18 Ckd株式会社 Piping-welded structure
EP4194542A1 (en) 2021-12-07 2023-06-14 Fluigent Apparatus for feeding a liquid medium to a fluidic system
WO2023240112A2 (en) * 2022-06-08 2023-12-14 Georgia Tech Research Corporation Enabling roll-to-roll manufacture of gradient thin film with multifunctional properties
WO2024013232A1 (en) * 2022-07-13 2024-01-18 Treefrog Therapeutics Connection device
FR3137953A1 (en) * 2022-07-13 2024-01-19 Treefrog Therapeutics Connection device
WO2024030286A1 (en) * 2022-08-02 2024-02-08 Illumina, Inc. Non-contact dispensers and related systems and methods

Family Cites Families (125)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3745091A (en) 1970-11-18 1973-07-10 Miles Lab Biological reaction chamber apparatus
US3726764A (en) 1971-08-06 1973-04-10 Miles Lab Microbiological chamber apparatus
US3879106A (en) 1973-04-11 1975-04-22 Pelam Inc Microscope slide cover slip
US3891327A (en) 1973-11-01 1975-06-24 Grace W R & Co Mounted slides and method of securing a cover glass to a glass slide having a specimen thereon
US4171866A (en) 1978-04-20 1979-10-23 Tolles Walter E Disposable volumetric slide
US4248904A (en) * 1979-05-21 1981-02-03 Fenimore David C Method for preparing samples for application to a thin layer chromatographic sheet
US4426451A (en) 1981-01-28 1984-01-17 Eastman Kodak Company Multi-zoned reaction vessel having pressure-actuatable control means between zones
US4505557A (en) 1982-08-16 1985-03-19 Helena Laboratories, Inc. Microscope slide
US4494912A (en) 1982-09-16 1985-01-22 Pauliukonis Richard S Energy conserving air pump
US4447140A (en) 1982-09-29 1984-05-08 Campbell Jeptha E Microscope slides
US4441793A (en) 1983-01-10 1984-04-10 Elkins Carlos D Microscopic evaluation slide
US4526690A (en) 1983-02-04 1985-07-02 Millipore Corporation Apparatus for nucleic acid quantification
US4673657A (en) * 1983-08-26 1987-06-16 The Regents Of The University Of California Multiple assay card and system
US4687423A (en) 1985-06-07 1987-08-18 Ivac Corporation Electrochemically-driven pulsatile drug dispenser
USRE33826E (en) 1985-07-23 1992-02-18 Hycor Biomedical, Inc. Microscope inspection slide
JPS6238408A (en) 1985-08-13 1987-02-19 Fuji Photo Film Co Ltd Cover film for microscope
DE3530993A1 (en) 1985-08-30 1987-03-05 Miles Lab TEST STRIP WITH DETERMINABLE SAMPLE CAPACITY
US5023187A (en) 1985-09-13 1991-06-11 Fisher Scientific Company Method and device for accelerated treatment of thin sample on surface
US4790640A (en) 1985-10-11 1988-12-13 Nason Frederic L Laboratory slide
US4948564A (en) 1986-10-28 1990-08-14 Costar Corporation Multi-well filter strip and composite assemblies
US4722598A (en) 1986-12-04 1988-02-02 Max M. Ford Diagnostic microscope slide having multiple sample wells and cover
US4849340A (en) 1987-04-03 1989-07-18 Cardiovascular Diagnostics, Inc. Reaction system element and method for performing prothrombin time assay
GB8722902D0 (en) 1987-09-30 1987-11-04 Shandon Southern Prod Tissue &c processing
US4908319A (en) 1988-03-16 1990-03-13 Smyczek Peter J Laboratory apparatus
US5100775A (en) 1988-03-16 1992-03-31 Smyczek Peter J Method for conducting nucleic acid hybridization in chamber with precise fluid delivery
US4911782A (en) 1988-03-28 1990-03-27 Cyto-Fluidics, Inc. Method for forming a miniaturized biological assembly
US5503803A (en) 1988-03-28 1996-04-02 Conception Technologies, Inc. Miniaturized biological assembly
US5200152A (en) 1988-03-28 1993-04-06 Cytonix Corporation Miniaturized biological assembly
SE462408B (en) 1988-11-10 1990-06-18 Pharmacia Ab OPTICAL BIOSENSOR SYSTEM USING SURFACE MONITORING RESONSE FOR THE DETECTION OF A SPECIFIC BIOMOLIC CYCLE, TO CALIBRATE THE SENSOR DEVICE AND TO CORRECT FOUND BASELINE OPERATION IN THE SYSTEM
US5346672A (en) 1989-11-17 1994-09-13 Gene Tec Corporation Devices for containing biological specimens for thermal processing
US5258163A (en) * 1990-04-14 1993-11-02 Boehringer Mannheim Gmbh Test carrier for analysis of fluids
US5192503A (en) 1990-05-23 1993-03-09 Mcgrath Charles M Probe clip in situ assay apparatus
US5100626A (en) 1990-05-24 1992-03-31 Levin Andrew E Binding assay device with removable cassette and manifold
US5061381A (en) 1990-06-04 1991-10-29 Abaxis, Inc. Apparatus and method for separating cells from biological fluids
SE9100392D0 (en) 1991-02-08 1991-02-08 Pharmacia Biosensor Ab A METHOD OF PRODUCING A SEALING MEANS IN A MICROFLUIDIC STRUCTURE AND A MICROFLUIDIC STRUCTURE COMPRISING SUCH SEALING MEANS
US5273905A (en) 1991-02-22 1993-12-28 Amoco Corporation Processing of slide mounted material
US5460945A (en) 1991-05-30 1995-10-24 Center For Blood Research, Inc. Device and method for analysis of blood components and identifying inhibitors and promoters of the inflammatory response
DE4132743A1 (en) * 1991-10-02 1993-04-08 Boehringer Mannheim Gmbh TEST CARRIER FOR THE ANALYSIS OF LIQUIDS
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5726026A (en) 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5393494A (en) 1992-05-28 1995-02-28 Diasys Corporation Apparatus for drawing fluid sample, components thereof, and slide assembly for use therewith
US5360741A (en) 1992-09-29 1994-11-01 Triangle Biomedical Sciences, Inc. DNA hybridization incubator
US5364790A (en) 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
DE4332720C2 (en) 1993-09-25 1997-02-13 Karlsruhe Forschzent Micro diaphragm pump
US6225059B1 (en) * 1993-11-01 2001-05-01 Nanogen, Inc. Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics
US5417576A (en) 1993-11-12 1995-05-23 Iowa Methodist Medical Center Means and method for microbiological growth and in situ observation with microscopes
US5466603A (en) 1994-02-15 1995-11-14 Meehan; Brian W. Temperature regulated hybridization chamber
US5571721A (en) 1994-05-05 1996-11-05 Erie Scientific Company Improved biological culture slide and method of making same
US5661029A (en) 1994-05-12 1997-08-26 Biomed Biological culture growth and observation device
US6287850B1 (en) * 1995-06-07 2001-09-11 Affymetrix, Inc. Bioarray chip reaction apparatus and its manufacture
EP0695941B1 (en) 1994-06-08 2002-07-31 Affymetrix, Inc. Method and apparatus for packaging a chip
US5639428A (en) 1994-07-19 1997-06-17 Becton Dickinson And Company Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay
IL115327A (en) 1994-10-07 2000-08-13 Bayer Ag Diaphragm pump
US5658413A (en) 1994-10-19 1997-08-19 Hewlett-Packard Company Miniaturized planar columns in novel support media for liquid phase analysis
US5578270A (en) 1995-03-24 1996-11-26 Becton Dickinson And Company System for nucleic acid based diagnostic assay
GB9506312D0 (en) 1995-03-28 1995-05-17 Medical Res Council Improvements in or relating to sample processing
US5605813A (en) 1995-06-06 1997-02-25 Becton, Dickinson And Company Culture slide assembly
US5518925A (en) 1995-06-06 1996-05-21 Becton Dickinson Co Culture slide assembly
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US6168948B1 (en) 1995-06-29 2001-01-02 Affymetrix, Inc. Miniaturized genetic analysis systems and methods
US20020022261A1 (en) * 1995-06-29 2002-02-21 Anderson Rolfe C. Miniaturized genetic analysis systems and methods
KR970706902A (en) 1995-09-12 1997-12-01 로드릭 리차드 제이 DEVICE AND METHOD FOR DNA AMPLIFICATION AND ASSAY
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US6132580A (en) * 1995-09-28 2000-10-17 The Regents Of The University Of California Miniature reaction chamber and devices incorporating same
US5863502A (en) * 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US5741647A (en) 1996-02-16 1998-04-21 Tam; Joseph Wing On Flow through nucleic acid hybridisation uses thereof and a device thereof
US6114122A (en) 1996-03-26 2000-09-05 Affymetrix, Inc. Fluidics station with a mounting system and method of using
US5962215A (en) * 1996-04-05 1999-10-05 Mercury Diagnostics, Inc. Methods for testing the concentration of an analyte in a body fluid
US6033544A (en) 1996-10-11 2000-03-07 Sarnoff Corporation Liquid distribution system
US6399023B1 (en) 1996-04-16 2002-06-04 Caliper Technologies Corp. Analytical system and method
US6144447A (en) 1996-04-25 2000-11-07 Pharmacia Biotech Ab Apparatus for continuously measuring physical and chemical parameters in a fluid flow
US5935524A (en) 1996-05-07 1999-08-10 E. I. Du Pont De Nemours And Company Holder for fluorometric samples
US6054277A (en) * 1996-05-08 2000-04-25 Regents Of The University Of Minnesota Integrated microchip genetic testing system
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
WO1997046313A1 (en) 1996-06-07 1997-12-11 Eos Biotechnology, Inc. Immobilised linear oligonucleotide arrays
NZ333346A (en) * 1996-06-28 2000-03-27 Caliper Techn Corp High-throughput screening assay systems in microscale fluidic devices
US6074827A (en) 1996-07-30 2000-06-13 Aclara Biosciences, Inc. Microfluidic method for nucleic acid purification and processing
JP2002515044A (en) * 1996-08-21 2002-05-21 スミスクライン・ビーチャム・コーポレイション A rapid method for sequencing and synthesizing bead-based combinatorial libraries
AU746549B2 (en) * 1996-11-20 2002-05-02 Becton Dickinson & Company Microfabricated isothermal nucleic acid amplification devices and methods
US5958341A (en) 1996-12-23 1999-09-28 American Registry Of Pathology Apparatus for efficient processing of tissue samples on slides
JP4663824B2 (en) 1996-12-31 2011-04-06 ハイ スループット ジェノミクス インコーポレイテッド Multiplexed molecular analyzer and method
US6052224A (en) 1997-03-21 2000-04-18 Northern Edge Associates Microscope slide system and method of use
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US5989499A (en) 1997-05-02 1999-11-23 Biomerieux, Inc. Dual chamber disposable reaction vessel for amplification reactions
US5922604A (en) 1997-06-05 1999-07-13 Gene Tec Corporation Thin reaction chambers for containing and handling liquid microvolumes
WO1999000655A2 (en) * 1997-06-27 1999-01-07 Immunetics, Inc. Rapid flow-through binding assay apparatus and method
US5876675A (en) 1997-08-05 1999-03-02 Caliper Technologies Corp. Microfluidic devices and systems
US5989402A (en) 1997-08-29 1999-11-23 Caliper Technologies Corp. Controller/detector interfaces for microfluidic systems
US6207031B1 (en) * 1997-09-15 2001-03-27 Whitehead Institute For Biomedical Research Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device
CA2301557A1 (en) * 1997-09-19 1999-04-01 Aclara Biosciences, Inc. Apparatus and method for transferring liquids
JP2001520377A (en) * 1997-10-15 2001-10-30 アクレイラ バイオサイエンシズ，インコーポレイティド Laminated micro structural device and method of manufacturing laminated micro structural device
US6096268A (en) * 1997-10-31 2000-08-01 Dade Behring Inc. Chromogenic and turbidimetric assay device
US6074725A (en) 1997-12-10 2000-06-13 Caliper Technologies Corp. Fabrication of microfluidic circuits by printing techniques
US6162639A (en) * 1997-12-19 2000-12-19 Amira Medical Embossed test strip system
US6037168A (en) 1997-12-31 2000-03-14 Cytonix Corporation Microbiological assembly comprising resealable closure means
US6167910B1 (en) 1998-01-20 2001-01-02 Caliper Technologies Corp. Multi-layer microfluidic devices
US6200814B1 (en) 1998-01-20 2001-03-13 Biacore Ab Method and device for laminar flow on a sensing surface
US6306347B1 (en) * 1998-01-21 2001-10-23 Bayer Corporation Optical sensor and method of operation
US6050719A (en) * 1998-01-30 2000-04-18 Affymetrix, Inc. Rotational mixing method using a cartridge having a narrow interior
US6251343B1 (en) * 1998-02-24 2001-06-26 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
WO2000009650A1 (en) * 1998-08-10 2000-02-24 Genomic Solutions, Inc. A thermal/fluidic cycling device for the purpose of nucleic acid hybridization
US6132685A (en) 1998-08-10 2000-10-17 Caliper Technologies Corporation High throughput microfluidic systems and methods
US6103199A (en) 1998-09-15 2000-08-15 Aclara Biosciences, Inc. Capillary electroflow apparatus and method
US6158712A (en) 1998-10-16 2000-12-12 Agilent Technologies, Inc. Multilayer integrated assembly having an integral microminiature valve
US6063579A (en) 1998-10-30 2000-05-16 Incyte Pharmaceuticals, Inc. Alignment mechanism
US6251601B1 (en) * 1999-02-02 2001-06-26 Vysis, Inc. Simultaneous measurement of gene expression and genomic abnormalities using nucleic acid microarrays
US6008893A (en) 1999-03-22 1999-12-28 Biacore Ab Reversible-flow conduit system
US6555361B1 (en) * 1999-03-24 2003-04-29 Corning Incorporated Hybridization chamber for high density nucleic acid arrays
WO2000060356A1 (en) * 1999-04-01 2000-10-12 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
DE60040977D1 (en) 1999-04-20 2009-01-15 Cytologix Corp LIQUID CHANGE IN A CHAMBER ON A MICROSCOPE CARRIER
US6225109B1 (en) * 1999-05-27 2001-05-01 Orchid Biosciences, Inc. Genetic analysis device
US6159727A (en) 1999-06-04 2000-12-12 Clontech Laboratories, Inc. Hybridization chamber
US6136592A (en) 1999-06-25 2000-10-24 Leighton; Stephen B. Multiple micro-arrays
US6399394B1 (en) * 1999-06-30 2002-06-04 Agilent Technologies, Inc. Testing multiple fluid samples with multiple biopolymer arrays
US6268219B1 (en) * 1999-07-09 2001-07-31 Orchid Biosciences, Inc. Method and apparatus for distributing fluid in a microfluidic device
US6272939B1 (en) * 1999-10-15 2001-08-14 Applera Corporation System and method for filling a substrate with a liquid sample
US6569674B1 (en) * 1999-12-15 2003-05-27 Amersham Biosciences Ab Method and apparatus for performing biological reactions on a substrate surface
US6284531B1 (en) * 2000-01-12 2001-09-04 Hong Zhu Multi-compartment device for cultivating microorganisms
US6309875B1 (en) * 2000-02-29 2001-10-30 Agilent Technologies, Inc. Apparatus for biomolecular array hybridization facilitated by agitation during centrifuging
US6436662B1 (en) * 2000-04-04 2002-08-20 Digene Corporation Device and method for cytology slide preparation
US6623860B2 (en) * 2000-10-10 2003-09-23 Aclara Biosciences, Inc. Multilevel flow structures
WO2002073184A2 (en) * 2001-03-07 2002-09-19 Symyx Technologies, Inc. Gas chromatograph injection valve
WO2002072264A1 (en) * 2001-03-09 2002-09-19 Biomicro Systems, Inc. Method and system for microfluidic interfacing to arrays
EP1384022A4 (en) * 2001-04-06 2004-08-04 California Inst Of Techn Nucleic acid amplification utilizing microfluidic devices
US7318912B2 (en) * 2001-06-07 2008-01-15 Nanostream, Inc. Microfluidic systems and methods for combining discrete fluid volumes

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014083799A1 (en) * 2012-11-27 2014-06-05 日本電気株式会社 Fluidic chip and waste liquid processing method for same
US11268632B2 (en) 2013-09-09 2022-03-08 Efficient Robotics Gmbh Microfluidic analysis component and production method

Also Published As

Publication number Publication date
EP1372848A4 (en) 2006-08-09
KR20030094280A (en) 2003-12-11
WO2002072264A1 (en) 2002-09-19
US20040037739A1 (en) 2004-02-26
EP1372848A1 (en) 2004-01-02
US7235400B2 (en) 2007-06-26
US20020192701A1 (en) 2002-12-19
JP2004533605A (en) 2004-11-04
JP4148778B2 (en) 2008-09-10
US7223363B2 (en) 2007-05-29
US20050019898A1 (en) 2005-01-27
KR100916074B1 (en) 2009-09-08
CA2450676C (en) 2010-03-30

Similar Documents

Publication Publication Date Title
CA2450676A1 (en) Method and system for microfluidic interfacing to arrays
EP1345697B1 (en) Microfluidic devices and methods for chemical assays
AU2002223871A1 (en) Microfluidic devices and methods for chemical assays
JP4721414B2 (en) REACTION CARTRIDGE, REACTOR, AND METHOD FOR TRANSFERRING REACTION CARTRIDGE SOLUTION
US7390463B2 (en) Microcolumn-based, high-throughput microfluidic device
JP4225972B2 (en) Microfluidic device and method comprising a purification column with excess diluent
US6729352B2 (en) Microfluidic synthesis devices and methods
EP1691924B1 (en) A modular biochip assembly
US20060002827A1 (en) Liquid reservoir connector
US20080131327A1 (en) System and method for interfacing with a microfluidic chip
US20080311585A1 (en) System and method for multiplex liquid handling
WO2003015923A1 (en) Fluid mixing in low aspect ratio chambers
US8323573B2 (en) Microfluidic cartridge with solution reservoir-pump chamber
KR20090056980A (en) A method and apparatus for attaching a fluid cell to a planar substrate
CN102448611A (en) Device and method for controlling fluid flows in lab-on-a-chip systems and method for producing said device
GB2386846A (en) Tensioning the laminates of a fluid device using a rim
WO2003015922A1 (en) Laminated microarray interface device
CN113164951B (en) Sample processing system, apparatus and method using semiconductor detection chip
CN117384750B (en) Fully integrated digital nucleic acid analysis cartridge
US20220323963A1 (en) Sample processing cartridge for use with a dna sequencer
US20240058813A1 (en) Fluid delivery device with passive pressure equilibration
JP2008139129A (en) Channel device
WO2022136248A1 (en) Analysis system for testing a sample
Viola et al. Surface effects at polymeric interfaces in capillary driving process

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20140310