CA2432538A1 - Method of disrupting interactions between biotin and biotin-binding compounds - Google Patents
Method of disrupting interactions between biotin and biotin-binding compounds Download PDFInfo
- Publication number
- CA2432538A1 CA2432538A1 CA002432538A CA2432538A CA2432538A1 CA 2432538 A1 CA2432538 A1 CA 2432538A1 CA 002432538 A CA002432538 A CA 002432538A CA 2432538 A CA2432538 A CA 2432538A CA 2432538 A1 CA2432538 A1 CA 2432538A1
- Authority
- CA
- Canada
- Prior art keywords
- biotin
- conjugate
- aqueous solution
- support
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract 32
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 title claims abstract 27
- 229960002685 biotin Drugs 0.000 title claims abstract 16
- 235000020958 biotin Nutrition 0.000 title claims abstract 16
- 239000011616 biotin Substances 0.000 title claims abstract 16
- 150000001875 compounds Chemical class 0.000 title claims abstract 9
- 230000003993 interaction Effects 0.000 title 1
- 239000007864 aqueous solution Substances 0.000 claims abstract 9
- -1 biotin compound Chemical class 0.000 claims abstract 5
- 102000039446 nucleic acids Human genes 0.000 claims abstract 5
- 108020004707 nucleic acids Proteins 0.000 claims abstract 5
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 5
- 108090000623 proteins and genes Proteins 0.000 claims abstract 4
- 102000004169 proteins and genes Human genes 0.000 claims abstract 4
- 238000001514 detection method Methods 0.000 claims abstract 2
- 238000002955 isolation Methods 0.000 claims abstract 2
- 238000000746 purification Methods 0.000 claims abstract 2
- 238000000926 separation method Methods 0.000 claims abstract 2
- 239000007787 solid Substances 0.000 claims 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- 239000002738 chelating agent Substances 0.000 claims 1
- 150000005829 chemical entities Chemical class 0.000 claims 1
- 239000012153 distilled water Substances 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 239000008213 purified water Substances 0.000 claims 1
- 230000002441 reversible effect Effects 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 108090001008 Avidin Proteins 0.000 abstract 1
- 108010090804 Streptavidin Proteins 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Semiconductor Lasers (AREA)
Abstract
The present invention relates to a method of disrupting a conjugate comprising a biotin compound and a biotin-binding compound, said method comprising the step of contacting said conjugate with an effective amount of a substantially aqueous solution under conditions such that the conjugate is disrupted, preferably reversibly disrupted, thereby forming a biotin compound and a biotin-binding compound. In particular, the invention relates to a method of reversibly releasing a biotinylated moiety from a streptavidin ( or avidin) coated support. Uses of the methods in techniques of detection, identification, determination, purification, separation and/or isolation of target proteins or nucleic acid molecules are also encompassed by the present invention.
Claims (23)
1. A method of disrupting a conjugate comprising a biotin compound and a biotin-binding compound, said method comprising the step of contacting said conjugate with an effective amount of a substantially aqueous solution under conditions such that the conjugate is disrupted, thereby forming a biotin compound and a biotin-binding compound.
2. The method of claim 1 wherein said conjugate comprises a biotin-avidin or biotin-streptavidin linkage.
3. The method of claim 1 wherein said conjugate is reversibly disrupted.
4. The method of any one of claims 1 to 3, wherein said substantially aqueous solution contains at least 50% by volume of water
5. The method of any one of claims 1 to 4, wherein said substantially aqueous solution is substantially free of chelating agents.
6. The method of any one of claims 1 to 5, wherein said substantially aqueous solution is substantially free of monovalent and/or divalent salts.
7. The method of any one of claims 1 to 6, wherein said substantially aqueous solution is purified or distilled water.
8. The method of any one of claims 1 to 7, wherein said contacting step comprises incubation of said conjugate at a temperature in the range of from about 20 to 95°C, preferably at about 80°C.
9. The method of any one of claims 1 to 8, wherein said biotin compound is bound or linked to one or more biological or chemical entity.
10. The method of claim 9 wherein said biological entity is a nucleic acid molecule, a protein or a chimeric molecule.
11. The method of any one of claims 1 to 10 wherein either the biotin or biotin-binding compound is immobilised on a solid support.
12. The method of claim 11 wherein the biotin-binding compound is immobilised.
13. The method of claim 11 or claim 12 wherein the solid support is particulate.
14. The method of claim 13, wherein said solid support comprises magnetic beads.
15. The method of any one of claims 11 to 14 wherein the surface of said solid support is substantially hydrophilic.
16. The method of any one of claims 11 to 15 wherein following disruption of said conjugate the solid support is re-used one or more times.
17. The method of any one of claims 11 to 16, further comprising the additional step of re-conditioning the solid support so that the support can be re-used.
18. Use of the method as defined in any one of claims 1 to 17 in a technique of detection, identification, determination, purification, separation and/or isolation of target proteins or nucleic acid molecules.
19. The use as claimed in claim 18, wherein said target proteins or nucleic acids are biotinylated.
20. The use as claimed in claim 18 or claim 19, wherein said technique involves nucleic acid amplification or sequencing
21. A method of releasing a biotinylated moiety from a support comprising a biotin-binding compound, said method comprising the step of eluting said support with a substantially aqueous solution, whereby to effect release, preferably reversible release of said moiety.
22. A method of reversibly immobilising a biotinylated moiety, said method comprising the following steps:
(a) binding said biotinylated moiety to a support comprising a biotin-binding compound, and subsequently (b) eluting said support with a substantially aqueous solution, whereby to effect release of said biotinylated moiety.
(a) binding said biotinylated moiety to a support comprising a biotin-binding compound, and subsequently (b) eluting said support with a substantially aqueous solution, whereby to effect release of said biotinylated moiety.
23. The method of claim 21 or 22 wherein said substantially aqueous solution, support and elution conditions are as defined in any one of the preceding claims 2 to 15.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0102568.3 | 2001-02-01 | ||
| GBGB0102568.3A GB0102568D0 (en) | 2001-02-01 | 2001-02-01 | Method |
| PCT/GB2002/000466 WO2002061428A2 (en) | 2001-02-01 | 2002-02-01 | Method of disrupting interactions between biotin and biotin-binding compounds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2432538A1 true CA2432538A1 (en) | 2002-08-08 |
| CA2432538C CA2432538C (en) | 2012-03-20 |
Family
ID=9907949
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2432538A Expired - Fee Related CA2432538C (en) | 2001-02-01 | 2002-02-01 | Method of disrupting interactions between biotin and biotin-binding compounds |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7279285B2 (en) |
| EP (1) | EP1356290B1 (en) |
| JP (1) | JP4394879B2 (en) |
| AT (1) | ATE553379T1 (en) |
| AU (1) | AU2002228214B2 (en) |
| CA (1) | CA2432538C (en) |
| GB (1) | GB0102568D0 (en) |
| WO (1) | WO2002061428A2 (en) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2004210964A1 (en) * | 2003-02-11 | 2004-08-26 | University Of Washington | Stimuli-responsive polymer conjugates and related methods |
| DK1620568T3 (en) | 2003-04-24 | 2009-03-30 | Afshin Ahmadian | Analysis for allele-specific mutation detection |
| KR20090107502A (en) | 2006-12-28 | 2009-10-13 | 니뽄 다바코 산교 가부시키가이샤 | Use of thermostable biotin-binding protein and solid support having the protein bound thereto |
| US7981688B2 (en) | 2007-03-08 | 2011-07-19 | University Of Washington | Stimuli-responsive magnetic nanoparticles and related methods |
| CN101896816B (en) * | 2007-06-22 | 2014-08-20 | B.R.A.H.M.S有限公司 | Method for detecting analytes |
| DE102007044708A1 (en) | 2007-09-18 | 2009-03-19 | Eads Deutschland Gmbh | Apparatus and method for regenerating biosensors |
| CA2730528C (en) * | 2008-07-16 | 2018-06-12 | Kbi Biopharma, Inc. | Methods and systems for manipulating particles using a fluidized bed |
| KR20100122366A (en) * | 2009-05-12 | 2010-11-22 | 삼성전자주식회사 | Magnetic nanoparticles for nucleic acid sequencing and the method using the same |
| US8426214B2 (en) * | 2009-06-12 | 2013-04-23 | University Of Washington | System and method for magnetically concentrating and detecting biomarkers |
| US9080933B2 (en) | 2009-11-09 | 2015-07-14 | University Of Washington Through Its Center For Commercialization | Stimuli-responsive polymer diagnostic assay comprising magnetic nanoparticles and capture conjugates |
| US20110117668A1 (en) * | 2009-11-09 | 2011-05-19 | University Of Washington Through Its Center For Commercialization | Self-powered smart diagnostic devices |
| WO2012006116A2 (en) * | 2010-06-28 | 2012-01-12 | Life Technologies Corporation | Methods, workflows, kits, apparatuses, and computer program media for nucleic acid sample preparation for nucleic acid sequencing |
| CA2835942C (en) | 2011-05-19 | 2019-01-22 | Sequenom, Inc. | Products and processes for multiplex nucleic acid identification |
| ES2873850T3 (en) | 2013-08-19 | 2021-11-04 | Abbott Molecular Inc | Next Generation Sequencing Libraries |
| US11225658B2 (en) * | 2013-12-05 | 2022-01-18 | New England Biolabs, Inc. | Enrichment and sequencing of RNA species |
| WO2016120247A1 (en) * | 2015-01-26 | 2016-08-04 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Methods for a quantitative release of biotinylated peptides and proteins from streptavidin complexes |
| CN107787371B (en) | 2015-04-24 | 2022-02-01 | 基纳生物技术有限公司 | Parallel method for detecting and quantifying minor variants |
| AU2016250841A1 (en) | 2015-04-24 | 2017-11-02 | Agena Bioscience, Inc. | Multiplexed method for the identification and quantitation of minor alleles and polymorphisms |
| MA45489A (en) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | CELL CULTURE PROCESSES, ASSOCIATED KITS AND APPARATUS |
| BR112019022356A2 (en) | 2017-04-27 | 2020-05-26 | Juno Therapeutics Gmbh | OLIGOMERIC PARTICLE REAGENTS AND METHODS OF USE THEREOF |
| KR102653725B1 (en) * | 2018-01-29 | 2024-04-01 | 세인트 쥬드 칠드런즈 리써치 호스피탈, 인코포레이티드 | Methods for Nucleic Acid Amplification |
| WO2020225971A1 (en) * | 2019-05-08 | 2020-11-12 | 株式会社日立ハイテク | Automatic analysis device pretreatment method |
| WO2020242856A1 (en) * | 2019-05-31 | 2020-12-03 | The Penn State Research Foundation | Specific selection of immune cells using versatile display scaffolds |
| CN111676217B (en) * | 2019-09-12 | 2021-10-26 | 南京君华基因科技有限公司 | Method and system for improving nucleic acid yield and being compatible with subsequent reaction |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5387505A (en) | 1990-05-04 | 1995-02-07 | Eastman Kodak Company | Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage |
| US5395856A (en) * | 1991-05-16 | 1995-03-07 | Rohm And Haas Company | HPLC avidin monomer affinity resin |
| JPH0578369A (en) * | 1991-08-12 | 1993-03-30 | Mitsubishi Petrochem Co Ltd | Method for separating and recovering d-biotin |
| IL114149A0 (en) | 1995-06-14 | 1995-10-31 | Yeda Res & Dev | Modified avidin and streptavidin molecules and use thereof |
| US6022688A (en) * | 1996-05-13 | 2000-02-08 | Sequenom, Inc. | Method for dissociating biotin complexes |
| US7056661B2 (en) | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
| WO2001051067A1 (en) * | 2000-01-13 | 2001-07-19 | Baxter International Inc. | Multiple inactivated blood factor anticoagulant composition |
-
2001
- 2001-02-01 GB GBGB0102568.3A patent/GB0102568D0/en not_active Ceased
-
2002
- 2002-02-01 JP JP2002561943A patent/JP4394879B2/en not_active Expired - Fee Related
- 2002-02-01 CA CA2432538A patent/CA2432538C/en not_active Expired - Fee Related
- 2002-02-01 WO PCT/GB2002/000466 patent/WO2002061428A2/en active Application Filing
- 2002-02-01 EP EP02710163A patent/EP1356290B1/en not_active Expired - Lifetime
- 2002-02-01 AT AT02710163T patent/ATE553379T1/en active
- 2002-02-01 AU AU2002228214A patent/AU2002228214B2/en not_active Ceased
- 2002-02-01 US US10/470,846 patent/US7279285B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP4394879B2 (en) | 2010-01-06 |
| US20040077024A1 (en) | 2004-04-22 |
| JP2004527732A (en) | 2004-09-09 |
| ATE553379T1 (en) | 2012-04-15 |
| WO2002061428A2 (en) | 2002-08-08 |
| EP1356290B1 (en) | 2012-04-11 |
| EP1356290A2 (en) | 2003-10-29 |
| WO2002061428A8 (en) | 2003-11-27 |
| CA2432538C (en) | 2012-03-20 |
| US7279285B2 (en) | 2007-10-09 |
| WO2002061428A3 (en) | 2003-04-17 |
| GB0102568D0 (en) | 2001-03-21 |
| AU2002228214B2 (en) | 2007-01-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20150202 |