CA2390281A1 - Hematopoietic differentiation of human embryonic stem cells - Google Patents

Hematopoietic differentiation of human embryonic stem cells Download PDF

Info

Publication number
CA2390281A1
CA2390281A1 CA002390281A CA2390281A CA2390281A1 CA 2390281 A1 CA2390281 A1 CA 2390281A1 CA 002390281 A CA002390281 A CA 002390281A CA 2390281 A CA2390281 A CA 2390281A CA 2390281 A1 CA2390281 A1 CA 2390281A1
Authority
CA
Canada
Prior art keywords
embryonic stem
stem cells
human embryonic
hematopoietic differentiation
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002390281A
Other languages
French (fr)
Other versions
CA2390281C (en
Inventor
Dan S. Kaufman
James A. Thomson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2390281A1 publication Critical patent/CA2390281A1/en
Application granted granted Critical
Publication of CA2390281C publication Critical patent/CA2390281C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/122—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells for inducing tolerance or supression of immune responses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1394—Bone marrow stromal cells; whole marrow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Abstract

Disclosed herein are methods of obtaining human hematopoietic cells from human embryonic stem cells using mammalian stromal cells. Hematopoietic cells derived in this way are useful for creating cell cultures suitable for transplantation, transfusion, and other purposes.
CA002390281A 1999-11-08 2000-08-25 Hematopoietic differentiation of human embryonic stem cells Expired - Lifetime CA2390281C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/435,578 1999-11-08
US09/435,578 US6280718B1 (en) 1999-11-08 1999-11-08 Hematopoietic differentiation of human pluripotent embryonic stem cells
PCT/US2000/023469 WO2001034776A1 (en) 1999-11-08 2000-08-25 Hematopoietic differentiation of human embryonic stem cells

Publications (2)

Publication Number Publication Date
CA2390281A1 true CA2390281A1 (en) 2001-05-17
CA2390281C CA2390281C (en) 2005-10-25

Family

ID=23728955

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002390281A Expired - Lifetime CA2390281C (en) 1999-11-08 2000-08-25 Hematopoietic differentiation of human embryonic stem cells

Country Status (13)

Country Link
US (3) US6280718B1 (en)
EP (1) EP1228194A1 (en)
JP (3) JP2003513664A (en)
KR (2) KR20070047850A (en)
CN (1) CN1228443C (en)
AU (1) AU784928B2 (en)
BR (1) BR0015374A (en)
CA (1) CA2390281C (en)
IL (2) IL149387A0 (en)
IS (1) IS6373A (en)
NO (1) NO20022180L (en)
SE (1) SE526490C2 (en)
WO (1) WO2001034776A1 (en)

Families Citing this family (178)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
IL142748A0 (en) * 1998-11-09 2002-03-10 Univ Monash Embryonic stem cells
US10638734B2 (en) 2004-01-05 2020-05-05 Abt Holding Company Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof
US7015037B1 (en) 1999-08-05 2006-03-21 Regents Of The University Of Minnesota Multiponent adult stem cells and methods for isolation
US8252280B1 (en) 1999-08-05 2012-08-28 Regents Of The University Of Minnesota MAPC generation of muscle
US20030129745A1 (en) * 1999-10-28 2003-07-10 Robl James M. Gynogenetic or androgenetic production of pluripotent cells and cell lines, and use thereof to produce differentiated cells and tissues
US6280718B1 (en) * 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
US6602711B1 (en) * 2000-02-21 2003-08-05 Wisconsin Alumni Research Foundation Method of making embryoid bodies from primate embryonic stem cells
US6534052B1 (en) 2000-09-05 2003-03-18 Yong-Fu Xiao Cardiac function comprising implantation of embryonic stem cell in which differentiation has been initiated
US6607720B1 (en) 2000-09-05 2003-08-19 Yong-Fu Xiao Genetically altered mammalian embryonic stem cells, their living progeny, and their therapeutic application for improving cardiac function after myocardial infarction
US7311905B2 (en) 2002-02-13 2007-12-25 Anthrogenesis Corporation Embryonic-like stem cells derived from post-partum mammalian placenta, and uses and methods of treatment using said cells
ES2522890T3 (en) 2000-12-06 2014-11-19 Anthrogenesis Corporation Method to collect placental stem cells
US20040121460A1 (en) * 2001-01-24 2004-06-24 Lumelsky Nadya L Differentiation of stem cells to pancreatic endocrine cells
CA2438501C (en) * 2001-02-14 2014-09-16 Leo T. Furcht Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof
IL157350A0 (en) 2001-02-14 2004-02-19 Anthrogenesis Corp Post-partum mammalian placenta, its use and placental stem cells therefrom
US20030211605A1 (en) * 2001-05-01 2003-11-13 Lee Sang-Hun Derivation of midbrain dopaminergic neurons from embryonic stem cells
WO2003010303A1 (en) 2001-07-24 2003-02-06 Es Cell International Pte Ltd Methods of inducing differentiation of stem cells
JP2005500847A (en) * 2001-08-24 2005-01-13 アドバンスド セル テクノロジー、インコーポレイテッド Screening assay for identification of differentiation inducers and preparation of differentiated cells for cell therapy
DE10144326B4 (en) * 2001-09-10 2005-09-22 Siemens Ag Method and system for monitoring a tire air pressure
US6759244B2 (en) * 2001-11-08 2004-07-06 Art Institute Of New York And New Jersey, Inc. Composite blastocysts (CBs) from aggregates of dissociated cells of non-viable pre-embryos
JP2005508393A (en) * 2001-11-09 2005-03-31 アーテセル・サイエンシズ・インコーポレーテツド Methods and compositions for the use of stromal cells to support embryonic and adult stem cells
AU2012258384B2 (en) * 2001-12-07 2016-04-21 Asterias Biotherapeutics, Inc. Hematopoietic cells from human embryonic stem cells
AU2016206280B2 (en) * 2001-12-07 2018-05-17 Asterias Biotherapeutics, Inc. Hematopoietic cells from human embryonic stem cells
EP1463803B1 (en) * 2001-12-07 2018-02-14 Asterias Biotherapeutics, Inc. Hematopoietic cells from human embryonic stem cells
US20040224403A1 (en) * 2001-12-07 2004-11-11 Robarts Research Institute Reconstituting hematopoietic cell function using human embryonic stem cells
US7799324B2 (en) * 2001-12-07 2010-09-21 Geron Corporation Using undifferentiated embryonic stem cells to control the immune system
US20030217374A1 (en) * 2002-01-15 2003-11-20 Advanced Cell Technology Cloning B and T lymphocytes
US20030134422A1 (en) * 2002-01-16 2003-07-17 Sayre Chauncey Bigelow Stem cell maturation for all tissue lines
US20050170506A1 (en) * 2002-01-16 2005-08-04 Primegen Biotech Llc Therapeutic reprogramming, hybrid stem cells and maturation
US20050090004A1 (en) * 2003-01-16 2005-04-28 Sayre Chauncey B. Stem cell maturation for all tissue lines
US20040076617A1 (en) * 2002-03-18 2004-04-22 Lieber Jonathan Gregory Method for production of neutrophils and uses therefor
US7498171B2 (en) * 2002-04-12 2009-03-03 Anthrogenesis Corporation Modulation of stem and progenitor cell differentiation, assays, and uses thereof
GB0210741D0 (en) * 2002-05-10 2002-06-19 Medical Res Council Methods of therapy
WO2003100018A2 (en) 2002-05-24 2003-12-04 Advanced Cell Technology, Inc. A bank of stem cells for transplantation
US20040091936A1 (en) * 2002-05-24 2004-05-13 Michael West Bank of stem cells for producing cells for transplantation having HLA antigens matching those of transplant recipients, and methods for making and using such a stem cell bank
CN1668733A (en) * 2002-05-30 2005-09-14 细胞基因公司 Methods of using JNK or MKK inhibitors to modulate cell differentiation and to treat myeloproliferative disorders and myelodysplastic syndromes
WO2004015077A2 (en) * 2002-08-08 2004-02-19 University Of Georgia Research Foundation, Inc. Compositions and methods for neural differentiation of embryonic stem cells
EP1581636A4 (en) * 2002-10-16 2006-09-20 Advanced Cell Tech Inc Methods using gene trapped stem cells for marking pathways of stem cell differentiation and making and isolating differentiated cells
GB0224415D0 (en) * 2002-10-21 2002-11-27 Medical Res Council Compositions
KR101042448B1 (en) 2002-11-26 2011-06-16 안트로제네시스 코포레이션 Cytotherapeutics, cytotherapeutic units and methods for treatments using them
US20060040385A1 (en) * 2002-12-05 2006-02-23 Technion Research & Development Foundation Ltd. Cultured human pancreatic islets, and uses thereof
US20040110286A1 (en) * 2002-12-06 2004-06-10 The John P. Robarts Research Institute Method for making hematopoietic cells
AU2004212009B2 (en) * 2003-02-13 2010-07-29 Celularity Inc. Use of umbilical cord blood to treat individuals having a disease, disorder or condition
WO2004085616A2 (en) * 2003-03-24 2004-10-07 The Uab Research Foundation Regulation of self-renewal in stem cells
JP4865540B2 (en) 2003-04-08 2012-02-01 イエダ リサーチ アンド ディベロップメント カンパニー リミテッド Stem cells with increased sensitivity to chemoattractants and methods of producing and using the same
FR2853551B1 (en) 2003-04-09 2006-08-04 Lab Francais Du Fractionnement STABILIZING FORMULATION FOR IMMUNOGLOBULIN G COMPOSITIONS IN LIQUID FORM AND LYOPHILIZED FORM
JP2007535486A (en) * 2003-05-07 2007-12-06 ラ ホーヤ インスティチュート フォー モレキュラー メディシン Method for promoting functional recovery of endogenous or implanted or transplanted stem cells using high molecular weight hyaluronic acid
WO2005038010A2 (en) * 2003-10-16 2005-04-28 The University Court Of The University Of Edinburgh Control of es cell self-renewal and lineage specification, and medium therefor
IL158868A0 (en) * 2003-11-13 2004-05-12 Yeda Res & Dev Methods of generating and using stem cells enriched with immature primitive progenitor
AU2004291559B2 (en) * 2003-11-19 2008-10-16 Australian Stem Cell Centre Limited Methods for producing blood products from pluripotent cells in cell culture
US20070269412A1 (en) 2003-12-02 2007-11-22 Celavie Biosciences, Llc Pluripotent cells
EP1709151B1 (en) * 2003-12-02 2016-04-13 Celavie Biosciences, LLC Compositions and methods for propagation of neural progenitor cells
GB2441530B (en) * 2004-02-12 2009-09-23 Univ Newcastle Stem Cells
US20050232905A1 (en) * 2004-03-26 2005-10-20 Yeh Edward T Use of peripheral blood cells for cardiac regeneration
US20050221482A1 (en) * 2004-03-31 2005-10-06 Burt Richard K Methods and compositions for obtaining hematopoietic stem cells derived from embryonic stem cells and uses thereof
US7670596B2 (en) * 2004-04-23 2010-03-02 Bioe, Inc. Multi-lineage progenitor cells
US7622108B2 (en) * 2004-04-23 2009-11-24 Bioe, Inc. Multi-lineage progenitor cells
EP1745125A1 (en) 2004-05-14 2007-01-24 Becton, Dickinson and Company Cell culture environments for the serum-free expansion of mesenchymal stem cells
EP1809739B1 (en) 2004-07-13 2014-10-15 Asterias Biotherapeutics, Inc. Medium for growing human embryonic stem cells
CA2504451A1 (en) * 2004-08-10 2006-02-10 Geron Corporation Dendritic cell vaccines for treating cancer made from embryonic stem cells
WO2006040763A2 (en) 2004-10-12 2006-04-20 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells and methods of generating and using same
WO2006050330A2 (en) * 2004-11-01 2006-05-11 Wisconsin Alumni Research Foundation Platelets from stem cells
US20070077654A1 (en) * 2004-11-01 2007-04-05 Thomson James A Platelets from stem cells
EP1841857A1 (en) * 2004-12-30 2007-10-10 Stemlifeline, Inc. Methods and compositions relating to embryonic stem cell lines
US20060263879A1 (en) * 2004-12-30 2006-11-23 Stemlifeline, Inc. Methods and systems relating to embryonic stem cell lines
EP1856248A4 (en) * 2005-02-09 2010-01-20 Burnham Inst Medical Research Homogeneous neural precursor cells
JP2008539700A (en) 2005-05-04 2008-11-20 オーストラリアン ステム セル センター リミテッド Selection, culture and production of lineage restricted hematopoietic stem cells
US7811821B2 (en) * 2005-06-01 2010-10-12 Wisconsin Alumni Research Foundation Method of forming dendritic cells from embryonic stem cells
US8034613B2 (en) 2005-06-01 2011-10-11 Wisconsin Alumni Research Foundation Multipotent lymphohematopoietic progenitor cells
CN107189980B (en) * 2005-06-22 2021-07-09 阿斯特利亚斯生物治疗股份公司 Suspension culture of human embryonic stem cells
WO2007002136A2 (en) 2005-06-22 2007-01-04 Geron Corporation Differentiation of primate pluripotent stem cells to cardiomyocyte-lineage cells
US20070041948A1 (en) * 2005-07-20 2007-02-22 Seoul National University Industry Foundation Method for culturing and proliferating hematopoietic stem cells and progenitor cells using human endometrial cells
EP1957633B1 (en) 2005-10-13 2013-12-18 Anthrogenesis Corporation Immunomodulation using placental stem cells
ZA200804673B (en) 2005-12-13 2009-11-25 Univ Kyoto Nuclear reprogramming factor
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
CN103146640B (en) 2005-12-29 2015-09-09 人类起源公司 Placental stem cell populations
KR20080081088A (en) 2005-12-29 2008-09-05 안트로제네시스 코포레이션 Co-culture of placental stem cells and stem cells from a second source
EP2019858B1 (en) * 2006-04-17 2012-06-13 BioE LLC Differentiation of multi-lineage progenitor cells to respiratory epithelial cells
EP2064319B1 (en) 2006-08-28 2017-02-22 Yeda Research and Development Co. Ltd. Methods of generating glial and neuronal cells and use of same for the treatment of medical conditions of the cns
US20080108044A1 (en) * 2006-11-08 2008-05-08 Deepika Rajesh In vitro differentiation of hematopoietic cells from primate embryonic stem cells
EP2087101A2 (en) * 2006-11-24 2009-08-12 Regents of the University of Minnesota Endodermal progenitor cells
US7883698B2 (en) * 2007-01-17 2011-02-08 Maria Michejda Isolation and preservation of fetal hematopoietic and mesencymal system cells from non-controversial materials and/or tissues resulting from miscarriages and methods of therapeutic use
NZ578819A (en) 2007-02-12 2012-02-24 Anthrogenesis Corp Treatment of inflammatory diseases using placental stem cells
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
US9029147B2 (en) * 2007-06-15 2015-05-12 Massachusetts Institute Of Technology Methods and compositions for enhanced differentiation from embryonic stem cells
JP2008307007A (en) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth
CA2693827A1 (en) * 2007-07-25 2009-01-29 Bioe, Inc. Differentiation of multi-lineage progenitor cells to chondrocytes
ES2530995T3 (en) 2007-09-28 2015-03-09 Anthrogenesis Corp Tumor suppression using human placental perfusate and intermediate natural killer cells that come from human placenta
US9683232B2 (en) 2007-12-10 2017-06-20 Kyoto University Efficient method for nuclear reprogramming
JP5558097B2 (en) 2007-12-10 2014-07-23 国立大学法人京都大学 Efficient nuclear initialization method
EP3118308B1 (en) 2008-04-30 2021-03-03 SanBio, Inc. Neural regenerating cells with alterations in dna methylation
EP2268809B1 (en) 2008-05-02 2019-02-06 Kyoto University Method of nuclear reprogramming
WO2009143241A2 (en) * 2008-05-21 2009-11-26 Bioe, Inc. Differentiation of multi-lineage progenitor cells to pancreatic cells
DK2329012T3 (en) 2008-08-20 2020-08-24 Celularity Inc Treatment of stroke using isolated placental cells
US10104880B2 (en) 2008-08-20 2018-10-23 Celularity, Inc. Cell composition and methods of making the same
MX339068B (en) 2008-08-22 2016-05-10 Anthrogenesis Corp Methods and compositions for treatment of bone defects with placental cell populations.
NZ602455A (en) 2008-11-19 2014-03-28 Anthrogenesis Corp Amnion derived adherent cells
US20100209399A1 (en) * 2009-02-13 2010-08-19 Celavie Biosciences, Llc Brain-derived stem cells for repair of musculoskeletal system in vertebrate subjects
WO2010098079A1 (en) 2009-02-24 2010-09-02 学校法人金沢医科大学 Method for denucleating nucleated erythrocyte, and denucleation inducer
US9388381B2 (en) 2009-07-09 2016-07-12 Massachusetts Institute Of Technology Methods and compositions for increased safety of stem cell-derived populations
WO2011017315A2 (en) 2009-08-03 2011-02-10 Recombinetics, Inc. Methods and compositions for targeted gene modification
EP3255142A1 (en) 2009-10-19 2017-12-13 Cellular Dynamics International, Inc. Cardiomyocyte production
US9121007B2 (en) 2010-01-26 2015-09-01 Anthrogenesis Corporatin Treatment of bone-related cancers using placental stem cells
KR20190076060A (en) 2010-04-07 2019-07-01 안트로제네시스 코포레이션 Angiogenesis using placental stem cells
US8562973B2 (en) 2010-04-08 2013-10-22 Anthrogenesis Corporation Treatment of sarcoidosis using placental stem cells
EP2582793B1 (en) 2010-06-15 2017-09-06 Cellular Dynamics International, Inc. A compendium of ready-built stem cell models for interrogation of biological response
EP2593542B1 (en) 2010-07-13 2018-01-03 Anthrogenesis Corporation Methods of generating natural killer cells
US8765470B2 (en) 2010-08-04 2014-07-01 Cellular Dynamics International, Inc. Reprogramming immortalized B-cells to induced pluripotent stem cells
CN103168236B (en) 2010-08-23 2016-01-20 哈佛大学管理委员会 For the light genetics probe that membrane potential measures
WO2012048275A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
WO2012054896A1 (en) 2010-10-22 2012-04-26 Biotime Inc. Methods of modifying transcriptional regulatory networks in stem cells
AU2011352036A1 (en) 2010-12-31 2013-07-18 Anthrogenesis Corporation Enhancement of placental stem cell potency using modulatory RNA molecules
WO2012121971A2 (en) 2011-03-04 2012-09-13 The Regents Of The University Of California Locally released growth factors to mediate motor recovery after stroke
CA2829804A1 (en) 2011-03-29 2012-10-04 Geron Corporation Enriched populations of cardiomyocyte lineage cells from pluripotent stem cells
CN106692193B (en) 2011-04-06 2021-01-05 桑比欧公司 Methods and compositions for modulating peripheral immune function
US9040035B2 (en) 2011-06-01 2015-05-26 Anthrogenesis Corporation Treatment of pain using placental stem cells
US10865383B2 (en) 2011-07-12 2020-12-15 Lineage Cell Therapeutics, Inc. Methods and formulations for orthopedic cell therapy
WO2013028684A1 (en) 2011-08-23 2013-02-28 Wisconsin Alumni Research Foundation Angiohematopoietic progenitor cells
US9925221B2 (en) 2011-09-09 2018-03-27 Celularity, Inc. Treatment of amyotrophic lateral sclerosis using placental stem cells
EP2594295A1 (en) 2011-11-16 2013-05-22 Servicio Andaluz De Salud Nerve implants based on a compacted biomaterial containing cells
US9447378B2 (en) 2012-04-27 2016-09-20 Massachusetts Institute Of Technology Method for differentiating human embryonic stem cells into β-cells for the treatment of type I diabetes
JP5432322B2 (en) 2012-05-08 2014-03-05 株式会社大塚製薬工場 Mammalian cell suspension for prevention of pulmonary embolism containing trehalose
AU2013237760A1 (en) 2012-10-08 2014-04-24 Biotime, Inc. Differentiated progeny of clonal progenitor cell lines
US9763983B2 (en) 2013-02-05 2017-09-19 Anthrogenesis Corporation Natural killer cells from placenta
WO2014171486A1 (en) 2013-04-17 2014-10-23 日産化学工業株式会社 Medium composition and method for producing red blood cells using same
WO2014197421A1 (en) 2013-06-05 2014-12-11 Biotime, Inc. Compositions and methods for induced tissue regeneration in mammalian species
US9816070B2 (en) 2013-06-14 2017-11-14 Massachusetts Institute Of Technology Articles and methods for stem cell differentiation
RU2663793C2 (en) 2013-06-28 2018-08-09 Оцука Фармасьютикал Фэктори, Инк. Trehalose and dextran-containing solution for transplanting mammalian cells
US10633625B2 (en) 2013-11-16 2020-04-28 Terumo Bct, Inc. Expanding cells in a bioreactor
CN103740642A (en) * 2014-01-28 2014-04-23 娄彩玲 Method for preparing human erythrocytes
US11078462B2 (en) 2014-02-18 2021-08-03 ReCyte Therapeutics, Inc. Perivascular stromal cells from primate pluripotent stem cells
EP3122866B1 (en) 2014-03-25 2019-11-20 Terumo BCT, Inc. Passive replacement of media
US10240127B2 (en) 2014-07-03 2019-03-26 ReCyte Therapeutics, Inc. Exosomes from clonal progenitor cells
JP6830059B2 (en) 2014-09-26 2021-02-17 テルモ ビーシーティー、インコーポレーテッド Scheduled cell feeding
ES2880353T3 (en) 2014-10-24 2021-11-24 Sumitomo Dainippon Pharma Co Ltd Retinal tissue production method
US10286009B2 (en) * 2015-05-16 2019-05-14 Asterias Biotherapeutics, Inc. Pluripotent stem cell-derived oligodendrocyte progenitor cells for the treatment of spinal cord injury
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
IL257794B (en) 2015-09-08 2022-09-01 Sumitomo Pharma Co Ltd Method for producing retinal pigment epithelial cells
CN115433707A (en) 2015-12-07 2022-12-06 再生疗法有限公司 Method for the re-derivation of brown adipocytes derived from different pluripotent stem cells
EP3423158B1 (en) 2016-02-24 2023-11-15 The Rockefeller University Embryonic cell-based therapeutic candidate screening systems, models for huntington's disease and uses thereof
EP3436567A4 (en) 2016-03-30 2019-10-30 Asterias Biotherapeutics, Inc. Oligodendrocyte progenitor cell compositions
EP3464565A4 (en) 2016-05-25 2020-01-01 Terumo BCT, Inc. Cell expansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
JP7006943B2 (en) 2016-11-04 2022-01-24 国立大学法人 東京大学 Mesenchymal cells and mesenchymal stem cells cryopreservation solution, frozen product, and cryopreservation method
AU2017363689B2 (en) 2016-11-25 2023-11-23 Riken Cell population for transplantation and method for producing same
KR102253850B1 (en) 2016-12-14 2021-05-20 가부시기가이샤오오쓰까세이야꾸고오죠오 Cryopreservation solution for mammalian cells
CA3055706A1 (en) 2017-03-08 2018-09-13 Sumitomo Dainippon Pharma Co., Ltd. Method for producing retinal pigment epithelial cells
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
WO2018184028A2 (en) 2017-03-31 2018-10-04 Terumo Bct, Inc. Cell expansion
KR20200029479A (en) 2017-07-20 2020-03-18 고쿠리쓰 겐큐 가이하쓰 호징 리가가쿠 겐큐소 Methods of preservation of nerve tissue
CN110945119B (en) 2017-07-20 2024-04-05 国立研究开发法人理化学研究所 Method for maturation of retinal tissue comprising continuous epithelium
WO2019050015A1 (en) 2017-09-08 2019-03-14 国立研究開発法人理化学研究所 Cell aggregate including retinal tissue and production method therefor
EP3683306A4 (en) 2017-09-14 2021-06-09 Riken Method for producing retinal tissues
JPWO2019054515A1 (en) 2017-09-14 2020-10-15 国立研究開発法人理化学研究所 Method of increasing pyramidal photoreceptors or rod photoreceptors by dorsal or ventral signaling
SG11202004831QA (en) 2017-11-24 2020-06-29 Sumitomo Chemical Co Production method for cell mass including neural cells/tissue and non-neural epithelial tissue, and cell mass from same
CN111386336A (en) 2017-11-24 2020-07-07 住友化学株式会社 Method for producing cell mass containing pituitary tissue and cell mass
WO2019160148A1 (en) 2018-02-19 2019-08-22 大日本住友製薬株式会社 Cell aggregate, mixture of cell aggregates, and method for preparing same
EP3842527A4 (en) 2018-08-24 2022-05-18 Sumitomo Chemical Company Limited Cell aggregation including olfactory neuron or precursor cell thereof, and method for producing same
US20210345602A1 (en) 2018-09-28 2021-11-11 Otsuka Pharmaceutical Factory, Inc Mammal cell preserving solution containing acarbose or stachyose
WO2020100481A1 (en) 2018-11-15 2020-05-22 Jsr株式会社 Method for producing brain organoids
SG11202106642YA (en) 2018-12-20 2021-07-29 Sumitomo Chemical Co Embryonic erythroblast-containing cell population and method for producing same, cell culture composition, and compound test method
WO2020138430A1 (en) 2018-12-28 2020-07-02 国立研究開発法人理化学研究所 Therapeutic drug for disease accompanied by disorders in retinal system cells or retinal tissue
US11603518B2 (en) 2019-01-23 2023-03-14 Asterias Biotherapeutics, Inc. Dorsally-derived oligodendrocyte progenitor cells from human pluripotent stem cells
WO2020184720A1 (en) 2019-03-13 2020-09-17 大日本住友製薬株式会社 Method for evaluating quality of transplant neural retina, and transplant neural retina sheet
JPWO2020218480A1 (en) 2019-04-26 2020-10-29
KR20210134701A (en) 2019-04-26 2021-11-10 가부시기가이샤오오쓰까세이야꾸고오죠오 Mammalian Cell Preservation Solution Containing Trehalose
WO2021045217A1 (en) 2019-09-06 2021-03-11 学校法人慶應義塾 Method for producing cell aggregate including glial progenitor cells
EP4063496A1 (en) 2019-11-20 2022-09-28 Sumitomo Pharma Co., Ltd. Method for freezing neural cells
CA3162273A1 (en) 2019-11-20 2021-05-27 Sumitomo Pharma Co., Ltd. Method for freezing cell aggregates
CN116157512A (en) 2020-09-11 2023-05-23 国立研究开发法人理化学研究所 Composite comprising cell aggregate containing neural retina and matrix, and method for producing same
WO2022054924A1 (en) 2020-09-11 2022-03-17 大日本住友製薬株式会社 Medium for tissue for transplantation
JPWO2022191216A1 (en) 2021-03-09 2022-09-15
CN117769591A (en) 2021-06-17 2024-03-26 国立大学法人京都大学 Method for producing human pluripotent stem cell-derived cerebral cortex cell preparation
AU2022343749A1 (en) 2021-09-13 2024-03-28 FUJIFILM Cellular Dynamics, Inc. Methods for the production of committed cardiac progenitor cells
WO2023049826A1 (en) 2021-09-23 2023-03-30 President And Fellows Of Harvard College Genetically encoded voltage indicators and uses thereof
WO2024073776A1 (en) 2022-09-30 2024-04-04 FUJIFILM Cellular Dynamics, Inc. Methods for the production of cardiac fibroblasts

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5635386A (en) * 1989-06-15 1997-06-03 The Regents Of The University Of Michigan Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture
NZ314644A (en) * 1993-05-24 2000-11-24 Immunex Corp Use of flt3-ligands as a growth stimulator of stem cells in the transplantation of tissue
US5914268A (en) 1994-11-21 1999-06-22 National Jewish Center For Immunology & Respiratory Medicine Embryonic cell populations and methods to isolate such populations
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
JP2001500725A (en) 1996-08-19 2001-01-23 ユニヴァーシティー オヴ マサチューセッツ Embryonic or stem cell-like cell lines produced by interspecies nuclear transfer
US6280718B1 (en) * 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells

Also Published As

Publication number Publication date
JP2003513664A (en) 2003-04-15
CN1387565A (en) 2002-12-25
SE526490C2 (en) 2005-09-27
JP2010042011A (en) 2010-02-25
US20040043484A1 (en) 2004-03-04
WO2001034776A1 (en) 2001-05-17
SE0201328L (en) 2002-07-05
CA2390281C (en) 2005-10-25
SE0201328D0 (en) 2002-05-03
AU784928B2 (en) 2006-07-27
IS6373A (en) 2002-05-06
BR0015374A (en) 2002-12-24
CN1228443C (en) 2005-11-23
US6280718B1 (en) 2001-08-28
EP1228194A1 (en) 2002-08-07
AU6940400A (en) 2001-06-06
JP2014000096A (en) 2014-01-09
US6613568B2 (en) 2003-09-02
IL149387A0 (en) 2002-11-10
US20020015694A1 (en) 2002-02-07
KR20070047850A (en) 2007-05-07
NO20022180D0 (en) 2002-05-07
KR20030022766A (en) 2003-03-17
IL149387A (en) 2008-08-07
NO20022180L (en) 2002-06-03

Similar Documents

Publication Publication Date Title
CA2390281A1 (en) Hematopoietic differentiation of human embryonic stem cells
WO2003014313A3 (en) Alternative compositions and methods for the culture of stem cells
IL210618A (en) Cell cultures comprising isolated expanded human non-embryonic non-germ cells, pharmaceutical compositions comprising the same, methods for their preparation and methods for identifying modulators and differentiation factors
WO2001036589A3 (en) Human ex vivo immune system
CA2402299A1 (en) Serum free cultivation of primate embryonic stem cells
EP1983044A3 (en) Animal cell culture media comprising plant-derived nutrients
CA2442135A1 (en) Methods to culture circovirus
EP1627914A3 (en) Methods and devices for the long-term culture of hematopoietic progenitor cells
AU2002250670A1 (en) Improved culture vessel having non-flat surfaces for growing or culturing cells, cellular aggregates, tissues and organoids and methods for using same
JP2003513664A5 (en)
EP1650307A3 (en) Methods for making recombinant proteins using apoptosis inhibitors
CA2388811A1 (en) Techniques for growth and differentiation of human pluripotent stem cells
NO20033396D0 (en) Improved in vitro method for culturing mammalian cells for autologous cell implantation / transplantation methods
WO2004055155A3 (en) Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same
EP1449916A3 (en) Submucosa as a growth substrate for cells
WO2000006704A3 (en) Hematopoietic cell composition for use in transplantation
WO2002072762A3 (en) Use of rna interference for the creation of lineage specific es and other undifferentiated cells and production of differentiated cells in vitro by co-culture
WO2005028626A3 (en) Cell culture media
BR0015148A (en) Androgenetic or gynogenetic production of pluripotent cell and cell lines, and their use to produce differentiated tissues and cells
CA2146734A1 (en) Method for long term subculture of dermal papilla cells
IL129966A (en) ISOLATED HUMAN EMBRYOID BODIES (hEB) DERIVED FROM HUMAN EMBRYONIC STEM CELLS
WO2000070022A3 (en) Ex vivo expansion of mammalian hematopoietic stem cells
CA2450789A1 (en) Schwann cells originating in myeloid interstitial cells
GB9825096D0 (en) Cells,culture methods and their uses
EP1702979A3 (en) Improved keratinocyte culture and uses thereof

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20200825