CA2383955A1 - Extracorporeal endotoxin removal method - Google Patents
Extracorporeal endotoxin removal method Download PDFInfo
- Publication number
- CA2383955A1 CA2383955A1 CA002383955A CA2383955A CA2383955A1 CA 2383955 A1 CA2383955 A1 CA 2383955A1 CA 002383955 A CA002383955 A CA 002383955A CA 2383955 A CA2383955 A CA 2383955A CA 2383955 A1 CA2383955 A1 CA 2383955A1
- Authority
- CA
- Canada
- Prior art keywords
- oligopeptides
- adsorbent
- polydisperse
- arginine
- blood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract 9
- 238000011013 endotoxin removal Methods 0.000 title claims 3
- 102000015636 Oligopeptides Human genes 0.000 claims abstract 17
- 108010038807 Oligopeptides Proteins 0.000 claims abstract 17
- 150000001413 amino acids Chemical class 0.000 claims abstract 16
- 239000003463 adsorbent Substances 0.000 claims abstract 14
- 239000004475 Arginine Substances 0.000 claims abstract 11
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims abstract 11
- 210000004369 blood Anatomy 0.000 claims abstract 11
- 239000008280 blood Substances 0.000 claims abstract 11
- 239000003446 ligand Substances 0.000 claims abstract 11
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract 6
- 239000004472 Lysine Substances 0.000 claims abstract 6
- 239000002158 endotoxin Substances 0.000 claims abstract 6
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims abstract 6
- 238000001179 sorption measurement Methods 0.000 claims abstract 3
- 239000000203 mixture Substances 0.000 claims 10
- 239000007790 solid phase Substances 0.000 claims 8
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 6
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Natural products C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims 6
- 239000000741 silica gel Substances 0.000 claims 6
- 229910002027 silica gel Inorganic materials 0.000 claims 6
- 238000004809 thin layer chromatography Methods 0.000 claims 6
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims 5
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims 5
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims 5
- 239000011324 bead Substances 0.000 claims 3
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical group CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 claims 2
- 210000000601 blood cell Anatomy 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 230000008878 coupling Effects 0.000 claims 2
- 238000010168 coupling process Methods 0.000 claims 2
- 238000005859 coupling reaction Methods 0.000 claims 2
- 108010072542 endotoxin binding proteins Proteins 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 1
- 102000004127 Cytokines Human genes 0.000 abstract 1
- 108090000695 Cytokines Proteins 0.000 abstract 1
- 206010040070 Septic Shock Diseases 0.000 abstract 1
- 238000002635 electroconvulsive therapy Methods 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000006068 polycondensation reaction Methods 0.000 abstract 1
- 230000036303 septic shock Effects 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
- B01D67/0081—After-treatment of organic or inorganic membranes
- B01D67/0093—Chemical modification
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
- B01D71/06—Organic material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
- A61L2/0017—Filtration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0082—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using chemical substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
- B01D69/14—Dynamic membranes
- B01D69/141—Heterogeneous membranes, e.g. containing dispersed material; Mixed matrix membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
- B01J20/3206—Organic carriers, supports or substrates
- B01J20/3208—Polymeric carriers, supports or substrates
- B01J20/321—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
- B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
- B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
- B01J20/3268—Macromolecular compounds
- B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
- B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G69/00—Macromolecular compounds obtained by reactions forming a carboxylic amide link in the main chain of the macromolecule
- C08G69/02—Polyamides derived from amino-carboxylic acids or from polyamines and polycarboxylic acids
- C08G69/08—Polyamides derived from amino-carboxylic acids or from polyamines and polycarboxylic acids derived from amino-carboxylic acids
- C08G69/10—Alpha-amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3679—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits by absorption
Abstract
This invention describes blood treating material having the capacity to selectively remove endotoxin and cytokine inducing substances from blood or plasma by extracorporeal adsorption for therapeutic septic shock treatment. The endotoxin adsorption ligands of the invention are oligopeptides synthesized from amino acids having a pk > 7.2 such as arginine, lysine o r histidine, using a polycondensation step such that the resultant oligopeptid es exhibit a high degree of polydispersity. Also provided are methods and devic es using an adsorbent having a polydisperse oligopeptide of the invention immobilized on a solid state support medium for removing endotoxin from the blood of human or animal subject.
Claims (26)
1. A composition consisting essentially of a mixture of linear or branched oligopeptides composed of one or more amino acids having a pK > 7.2, said oligopeptides being polydisperse with respect to molecular weight and to number of branches per molecule.
2. The composition of claim 1 wherein the amino acids are selected from the group consisting of arginine, lysine and histidine.
3. The composition of claim 1 wherein said oligopeptides are polydisperse with an R f value of 0.4 or greater as measured by thin layer chromatography on silica gel using a system of CH3Cl3: CH3OH: NH3/40:40:20 in 45% NH3 solution.
4. The composition of claim 1 wherein said oligopeptides are polydisperse with an R f value of 0.6 or greater as measured by thin layer chromatography on silica gel using a system of CH3Cl3:CH3OH:NH3/40:40:20 in 45%NH3 solution.
5. The composition of claims 1, 2, 3 or 4 wherein the amino acid is arginine.
6. An endotoxin removal adsorbent comprising a ligand immobilized on a solid phase support medium, the ligand consisting essentially of a mixture of linear or branched oligopeptides composed of one or more amino acids having a pK > 7.2, said oligopeptides being polydisperse with respect to molecular weight and to number of branches per molecule.
7. The adsorbent of claim 6 wherein the solid phase support medium is porous sufficient to allow passage of blood cells therethrough.
8. The adsorbent of claim 6 wherein the solid phase support medium is in the form of beads.
9. The adsorbent of claim 6 wherein the ligand is covalently bound to the solid phase support medium.
10. The adsorbent of claims 6, 8 or 9 wherein the amino acid is selected from the group consisting of arginine, lysine and histidine.
11. The adsorbent of claims 8 or 9 wherein said oligopeptides are polydisperse with an R f value of 0.4 or greater as measured by thin layer chromatography on silica gel using a system of CH3Cl3:CH3OH:NH3/40:40:20 in 45% NH3 solution.
12. The adsorbent of claims 8 or 9 wherein said oligopeptides are polydisperse with an R f value of 0.6 or greater as measured by thin layer chromatography on silica gel using a system of CH3Cl3:CH3OH:NH3/40:40:20 in 45% NH3 solution.
13. The adsorbent of claims 8, 9, 11 or 12 wherein the amino acid is arginine.
14. A device for extracorporeal removal of endotoxin from whole blood comprising a container containing and retaining an endotoxin removal adsorbent comprising a ligand immobilized on a solid phase support medium, the ligand consisting essentially of a mixture of linear or branched oligopeptides composed of one or more amino acids having a pK > 7.2, said oligopeptides being polydisperse with respect to molecular weight and to number of branches per molecule, said solid phase support medium being porous sufficient to allow passage of blood cells therethrough, said container having an inlet and an outlet positioned with respect to the adsorbent such that blood entering the inlet contacts the adsorbent before exiting the container through the outlet.
15. The device of claim 14 wherein the solid phase support medium is in the form of beads.
16. The device of claim 15 wherein the ligand is covalently bound to the beads, and the amino acid is selected from the group consisting of arginine, lysine and histidine.
17. The device of claim 14 wherein said oligopeptides are polydisperse with an R f value of 0.4 or greater as measured by thin layer chromatography on silica gel using a system of CH3Cl3:CH3OH:NH3/40:40:20 in 45% NH3 solution.
18. The device of claim 14 wherein said oligopeptides are polydisperse with an R f value of 0.6 or greater as measured by thin layer chromatography on silica gel using a system of CH3Cl3:CH3OH:NH3/40:40:20 in 45% NH3 solution.
19. The device of claims 16, 17 or 18 wherein the amino acid is arginine.
20. A method for removing endotoxin from blood of an animal or human subject comprising removing a portion of blood from the subject, contacting the blood with an adsorbent comprising a ligand immobilized on a solid phase support medium, the ligand consisting essentially of a mixture of linear or branched oligopeptides composed of one or more amino acids having a pK > 7.2, said oligopeptides being polydisperse with respect to molecular weight and to number of branches per molecule, whereby endotoxin is removed from the blood by adsorption to said adsorbent, then returning the blood to the subject.
21. The method of claim 20 wherein the steps of removing, contacting and returning blood are carried out in a continuous flow from and to the subject.
22. The method of claims 20 or 21 wherein the amino acid is selected from the group consisting of arginine, lysine and histidine.
23. The method of claim 22 wherein the amino acid is arginine.
24. A process for making an endotoxin-binding ligand, comprising reacting an amino acid selected from the group consisting of arginine, lysine and histidine with a coupling reagent at a pH selected to provide that a portion of basic groups of the amino acids are unprotonated, whereby a polydisperse, branched oligopeptide mixture is formed.
25. The process of claim 24 wherein the amino acid is arginine, the reaction pH is 12 and the coupling reagent is 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide.
26. An endotoxin-binding ligand made according to the method of claims 24 or 25.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15664999P | 1999-09-29 | 1999-09-29 | |
| US60/156,649 | 1999-09-29 | ||
| US09/677,375 US6774102B1 (en) | 1999-09-29 | 2000-09-15 | Extracorporeal endotoxin removal method |
| US09/677,375 | 2000-09-15 | ||
| PCT/IB2000/001364 WO2001023413A1 (en) | 1999-09-29 | 2000-09-26 | Extracorporeal endotoxin removal method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2383955A1 true CA2383955A1 (en) | 2001-04-05 |
| CA2383955C CA2383955C (en) | 2010-10-12 |
Family
ID=26853386
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2383955A Expired - Fee Related CA2383955C (en) | 1999-09-29 | 2000-09-26 | Extracorporeal endotoxin removal method |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6774102B1 (en) |
| EP (1) | EP1220868B1 (en) |
| JP (1) | JP4709452B2 (en) |
| AT (1) | ATE260294T1 (en) |
| CA (1) | CA2383955C (en) |
| DE (1) | DE60008565T2 (en) |
| ES (1) | ES2213604T3 (en) |
| WO (1) | WO2001023413A1 (en) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8197430B1 (en) * | 1998-05-22 | 2012-06-12 | Biopheresis Technologies, Inc. | Method and system to remove cytokine inhibitor in patients |
| US6620382B1 (en) | 1998-05-22 | 2003-09-16 | Biopheresis Technologies, Llc. | Method and compositions for treatment of cancers |
| US20020164644A1 (en) * | 2000-04-05 | 2002-11-07 | Ikuro Maruyama | Adsorbents for high mobility group proteins and column for purifying body fluid |
| WO2004024099A2 (en) * | 2002-04-02 | 2004-03-25 | Scantibodies Laboratory, Inc. | Methods and devices for treating severe peripheral bacterial infections |
| AU2008201015B2 (en) * | 2002-04-25 | 2008-11-13 | Alteco Medical Ab | Improved separation |
| SE0201257D0 (en) * | 2002-04-25 | 2002-04-25 | Medical Invest In Sweden Ab | Improved Separation |
| SE526038C2 (en) * | 2002-07-08 | 2005-06-21 | Gambro Lundia Ab | Polymer affinity matrix, process for its preparation and use thereof |
| US6803183B2 (en) | 2002-07-18 | 2004-10-12 | Clarigen, Inc. | Method for removing pyrogens from plasma and blood for treatment of septic shock |
| FR2848223B1 (en) * | 2002-12-09 | 2005-02-25 | Centre Nat Rech Scient | NOVEL METHOD OF ISOLATING ENDOTOXINS. |
| EP1518870B1 (en) * | 2003-09-17 | 2018-11-28 | Gambro Lundia AB | Separating material |
| US8142844B2 (en) * | 2003-09-17 | 2012-03-27 | Gambro Lundia Ab | Separating material |
| US20050103712A1 (en) * | 2003-11-13 | 2005-05-19 | Voyce Brian D. | Methods and devices for treating severe peripheral bacterial infections |
| PT1949915E (en) * | 2004-04-30 | 2012-11-27 | Biopheresis Technologies Inc | Method and system to remove soluble tnfr1, tnfr2, and il2r in patients |
| US20050287515A1 (en) * | 2004-06-24 | 2005-12-29 | Reinhold Deppisch | Removal of bacterial DNA from therapeutic fluid formulations |
| US7714111B2 (en) * | 2006-09-08 | 2010-05-11 | Wyeth Llc | Arginine derivative wash in protein purification using affinity chromatography |
| GB0621452D0 (en) * | 2006-10-27 | 2006-12-06 | Ucl Business Plc | Therapy for liver disease |
| GB0621454D0 (en) | 2006-10-27 | 2006-12-06 | Ucl Business Plc | Goal directed therapy for liver failure |
| ES2375314T3 (en) | 2007-02-28 | 2012-02-28 | Lipoxen Technologies Limited | REDUCTION OF ENDOTOXINE IN POLYSIALIC ACIDS. |
| GB0800228D0 (en) * | 2008-01-07 | 2008-02-13 | Novartis Ag | Lipopolysaccharide decontamination |
| WO2012154218A2 (en) * | 2011-05-09 | 2012-11-15 | The University Of Miami | Reducing soluble urokinase receptor in the circulation |
| CN111433605B (en) | 2017-09-18 | 2023-09-08 | 圣特萨斯股份公司 | Method and device for purifying blood against circulating cell-free DNA |
| GB202004775D0 (en) | 2020-03-31 | 2020-05-13 | Ucl Business Ltd | Apparatus |
| US20230347037A1 (en) | 2020-10-01 | 2023-11-02 | Alteco Medical Ab | Matrices for selective binding of at least one component from a body fluid |
| WO2022112603A1 (en) | 2020-11-30 | 2022-06-02 | Alteco Medical Ab | Device for binding and separation of at least one component from a body fluid |
| CA3227866A1 (en) | 2021-07-29 | 2023-02-02 | Japan Hemotech Co., Ltd. | Nonwoven substrate, fibrous material for liquid clarification, production method for said material, and cleaner equipped with said material |
| GB202307837D0 (en) | 2023-05-25 | 2023-07-12 | Yaqrit Ltd | Dialysis method |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5670028A (en) | 1979-11-12 | 1981-06-11 | Teijin Ltd | Polymer bonding with albumin |
| CA1204667A (en) | 1982-10-04 | 1986-05-20 | Masashi Kodama | Blood treating material |
| JPH0738880B2 (en) | 1983-06-24 | 1995-05-01 | 東レ株式会社 | Endotoxemia treatment |
| EP0299965A4 (en) * | 1986-03-24 | 1992-12-16 | University Of Sydney | Antigenic analogues of platelet activating factor (paf) |
| SE468881B (en) | 1991-01-09 | 1993-04-05 | Kabi Pharmacia Ab | APPLICATION OF CERTAIN PHARMACEUTICALS FOR THE PREPARATION OF MEDICINAL PRODUCTS FOR TREATMENT OF ENDOTOXIN-INDEXED EFFECTS AND THE PREPARATION OF ENDOTOXINES FROM MISCELLANEOUS SOLUTIONS |
| DE4110730A1 (en) | 1991-04-03 | 1992-10-15 | Fresenius Ag | DEVICE FOR SEPARATING AT LEAST TWO BIOLOGICAL SUBSTANCES IN SOLUTION BY ADSORPTION |
| GB9215780D0 (en) * | 1992-07-24 | 1992-09-09 | Univ London Pharmacy | Peptide compounds |
| CA2140257A1 (en) * | 1992-07-27 | 1994-02-03 | Lawrence M. Kauvar | Sorbent families |
| US5780594A (en) * | 1993-03-01 | 1998-07-14 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Biologically active protein fragments containing specific binding regions of serum albumin or related proteins |
| WO1995000540A1 (en) * | 1993-06-18 | 1995-01-05 | Robert Webber | Synthetic carrier and immunogen |
| JPH08503490A (en) | 1993-08-18 | 1996-04-16 | モルフォシス・ゲゼルシャフト・フュア・プロタインオプティミールング・ミット・ベシュレンクテル・ハフツング | Lipopolysaccharide-binding and neutralizing peptides |
| DE69525655T2 (en) | 1994-12-26 | 2002-09-12 | Kanegafuchi Chemical Ind | ADSORBENT FOR ENDOTOXIN, TUMOR NECROSE FACTOR-ALPHA OR INTERLEUKINE, METHOD FOR REMOVAL BY MEANS OF ADSORPTION AND ADSORBER |
| DE69623769T2 (en) | 1995-06-02 | 2003-02-20 | Optilink Ab Malmoe | PHYSICAL FUNCTIONAL MATERIALS |
| WO1996041185A1 (en) | 1995-06-07 | 1996-12-19 | Perseptive Biosystems, Inc. | Methods and compositions for binding endotoxins |
| EP0787500B1 (en) | 1996-02-06 | 1999-12-01 | BELLCO S.p.A. | Device for extracorporeal removal of toxins, in particular cytokines |
| DE19609479A1 (en) * | 1996-03-11 | 1997-09-18 | Biotechnolog Forschung Gmbh | Endotoxin-specific membranes |
| EP0884327B1 (en) * | 1997-06-11 | 2001-03-14 | The School Of Pharmacy, University Of London | Dendritic lysine-based polypeptides for targeted drug delivery |
| DE19740770A1 (en) * | 1997-09-17 | 1999-03-18 | Biotechnolog Forschung Gmbh | Microfiltration layer for removing endotoxins from liquids |
| CN1159583C (en) * | 1998-03-11 | 2004-07-28 | 医药及科学传感器公司 | Detection of analytes by fluoresce lanthanide chelates |
-
2000
- 2000-09-15 US US09/677,375 patent/US6774102B1/en not_active Expired - Lifetime
- 2000-09-26 DE DE60008565T patent/DE60008565T2/en not_active Expired - Lifetime
- 2000-09-26 ES ES00960909T patent/ES2213604T3/en not_active Expired - Lifetime
- 2000-09-26 AT AT00960909T patent/ATE260294T1/en not_active IP Right Cessation
- 2000-09-26 JP JP2001526563A patent/JP4709452B2/en not_active Expired - Lifetime
- 2000-09-26 CA CA2383955A patent/CA2383955C/en not_active Expired - Fee Related
- 2000-09-26 WO PCT/IB2000/001364 patent/WO2001023413A1/en active IP Right Grant
- 2000-09-26 EP EP00960909A patent/EP1220868B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES2213604T3 (en) | 2004-09-01 |
| JP4709452B2 (en) | 2011-06-22 |
| DE60008565T2 (en) | 2004-12-16 |
| CA2383955C (en) | 2010-10-12 |
| ATE260294T1 (en) | 2004-03-15 |
| WO2001023413A1 (en) | 2001-04-05 |
| US6774102B1 (en) | 2004-08-10 |
| EP1220868B1 (en) | 2004-02-25 |
| JP2003511354A (en) | 2003-03-25 |
| DE60008565D1 (en) | 2004-04-01 |
| EP1220868A1 (en) | 2002-07-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2383955A1 (en) | Extracorporeal endotoxin removal method | |
| Garipcan et al. | A novel affinity support material for the separation of immunoglobulin G from human plasma | |
| US4747956A (en) | Method of adsorbing subtances | |
| JP4945876B2 (en) | High mobility group protein adsorbent and body fluid purification column | |
| JP6469574B2 (en) | Functionalized granular carrier and its production and use | |
| Denizli et al. | Novel metal-chelate affinity adsorbent for purification of immunoglobulin-G from human plasma | |
| US3983001A (en) | Isolation of biologically active compounds by affinity chromatography | |
| US4523997A (en) | Affinity chromatography matrix with built-in reaction indicator | |
| JP3441496B2 (en) | Affinity separation material | |
| Öztürk et al. | Silane‐modified magnetic beads: application to immunoglobulin G separation | |
| JPH0489500A (en) | Method for purifying substance by affinity chromatography and apparatus for purification | |
| Derazshamshir et al. | Preparation of Zn2+‐chelated poly (HEMA‐MAH) cryogel for affinity purification of chicken egg lysozyme | |
| JP2928589B2 (en) | Adsorbent for modified LDL and apparatus for removing modified LDL using the same | |
| EP0382215A1 (en) | Porous adsorbent for beta-2-Microglobulin | |
| CA2055869A1 (en) | Optical isomer separating agents | |
| Yavuz et al. | Molecular recognition based iron removal from human plasma with imprinted membranes | |
| JP3970339B2 (en) | Cell separation material | |
| JP4422541B2 (en) | Temperature-responsive liquid chromatography carrier and liquid chromatography method using the same | |
| JPS6222659A (en) | Method and apparatus for regenerating low specific gravity lipoprotein adsorbing material | |
| Santiago et al. | A robust affinity chromatography system based on ceramic monoliths coated with poly (amino acid)‐based polymeric constructs | |
| RU2068703C1 (en) | Method of magnoinnune sorbent preparing | |
| SU1054353A1 (en) | Process for preparing adsorbents containing amino groups | |
| JPH02668B2 (en) | ||
| Yilmaz | Supermacroporous hydrophobic affinity sorbents for protein chromatography | |
| EP0610756A1 (en) | Activated membrane and method for ligate separation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20150928 |