CA2372665A1 - Process for high throughput dna methylation analysis - Google Patents

Process for high throughput dna methylation analysis Download PDF

Info

Publication number
CA2372665A1
CA2372665A1 CA002372665A CA2372665A CA2372665A1 CA 2372665 A1 CA2372665 A1 CA 2372665A1 CA 002372665 A CA002372665 A CA 002372665A CA 2372665 A CA2372665 A CA 2372665A CA 2372665 A1 CA2372665 A1 CA 2372665A1
Authority
CA
Canada
Prior art keywords
nucleic acid
genomic dna
high throughput
dna samples
specifically
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002372665A
Other languages
French (fr)
Other versions
CA2372665C (en
Inventor
Peter W. Laird
Cindy A. Eads
Kathleen D. Danenberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Southern California USC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23209034&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2372665(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Individual filed Critical Individual
Publication of CA2372665A1 publication Critical patent/CA2372665A1/en
Application granted granted Critical
Publication of CA2372665C publication Critical patent/CA2372665C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

There is disclosed an improved high-throughput and quantitative process for determining methylation patterns in genomic DNA samples based on amplifying modified nucleic acid, and detecting methylated nucleic acid based on amplification-dependent displacement of specifically annealed hybridization probes. Specifically, the inventive process provides for treating genomic DNA samples with sodium bisulfite to create methylation-dependent sequence differences, followed by detection with fluorescence-based quantitative PCR
techniques. The process is particularly well suited for the rapid analysis of a large number of nucleic acid samples, such as those from collections of tumor tissues.
CA002372665A 1999-05-14 2000-05-12 Process for high throughput dna methylation analysis Expired - Lifetime CA2372665C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/311,912 1999-05-14
US09/311,912 US6331393B1 (en) 1999-05-14 1999-05-14 Process for high-throughput DNA methylation analysis
PCT/US2000/013029 WO2000070090A1 (en) 1999-05-14 2000-05-12 Process for high throughput dna methylation analysis

Publications (2)

Publication Number Publication Date
CA2372665A1 true CA2372665A1 (en) 2000-11-23
CA2372665C CA2372665C (en) 2007-07-17

Family

ID=23209034

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002372665A Expired - Lifetime CA2372665C (en) 1999-05-14 2000-05-12 Process for high throughput dna methylation analysis

Country Status (11)

Country Link
US (4) US6331393B1 (en)
EP (2) EP1710321B1 (en)
JP (2) JP4044290B2 (en)
AT (1) ATE332980T2 (en)
AU (2) AU775798B2 (en)
CA (1) CA2372665C (en)
CY (1) CY1118000T1 (en)
DE (1) DE60029323T3 (en)
DK (2) DK1710321T3 (en)
ES (2) ES2267535T5 (en)
WO (1) WO2000070090A1 (en)

Families Citing this family (256)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
CA2404568A1 (en) * 2000-03-31 2001-10-11 University Of Southern California Epigenetic sequences for esophageal adenocarcinoma
EP1268857A2 (en) * 2000-04-06 2003-01-02 Epigenomics AG Diagnosis of diseases associated with gene regulation
ATE347619T1 (en) * 2000-08-25 2006-12-15 Lovelace Respiratory Res Inst METHOD FOR CANCER DETECTION USING NESTED (ßNESTEDß) METHYLATION-SPECIFIC PCR
US6844152B1 (en) 2000-09-15 2005-01-18 Promega Corporation Detection of microsatellite instability and its use in diagnosis of tumors
US20020058265A1 (en) * 2000-09-15 2002-05-16 Promega Corporation Detection of microsatellite instability and its use in diagnosis of tumors
GB0025913D0 (en) 2000-10-23 2000-12-06 Guldberg Per Materials and methods relating to nucleic acid amplification and profiling
CA2425904A1 (en) * 2000-10-23 2002-05-02 Cancer Research Technology Limited Materials and methods relating to nucleic acid amplification and profiling
US20030188326A1 (en) 2000-11-03 2003-10-02 Dana Farber Cancer Institute Methods and compositions for the diagnosis of cancer susceptibilities and defective DNA repair mechanisms and treatment thereof
DE10056802B4 (en) * 2000-11-14 2005-06-16 Epigenomics Ag Method for the detection of methylation conditions for toxicological diagnostics
DE10104937B4 (en) * 2001-01-29 2005-03-17 Epigenomics Ag Fluorescence polarization 2
US6893820B1 (en) * 2001-01-31 2005-05-17 The Ohio State University Research Foundation Detection of methylated CpG rich sequences diagnostic for malignant cells
WO2002070741A2 (en) * 2001-03-01 2002-09-12 Epigenomics Ag Methods, systems and computer program products for determining the biological effect and/or activity of drugs, chemical substances and/or pharmaceutical compositions based on their effect on the methylation status of the dna
JP2004529630A (en) * 2001-03-01 2004-09-30 エピゲノミクス アーゲー Methods for developing diagnostic and therapeutic gene panels based on gene expression and methylation status
DE10112515B4 (en) * 2001-03-09 2004-02-12 Epigenomics Ag Method for the detection of cytosine methylation patterns with high sensitivity
US20020197639A1 (en) * 2001-06-08 2002-12-26 Shia Michael A. Methods and products for analyzing nucleic acids based on methylation status
DE10128508A1 (en) 2001-06-14 2003-02-06 Epigenomics Ag Methods and nucleic acids for the differentiation of prostate tumors
DE10128509A1 (en) * 2001-06-14 2003-01-02 Epigenomics Ag Methods and nucleic acids for the differentiation of prostate and kidney carcinomas
DE10130800B4 (en) 2001-06-22 2005-06-23 Epigenomics Ag Method for the detection of cytosine methylation with high sensitivity
DE10132211A1 (en) * 2001-06-27 2003-01-16 Epigenomics Ag Detection of specific dinucleotides in DNA samples by fluorescence resonance energy transfer (FRET)
EP1418949B1 (en) 2001-07-31 2013-06-19 THE STATE OF OREGON acting by and through THE STATE BOARD OF HIGHER EDUCATION on behalf of THE UNIVERSITY OF OREGON Inhibitor of dna methylation
DE10139283A1 (en) 2001-08-09 2003-03-13 Epigenomics Ag Methods and nucleic acids for the analysis of colon cancer
US6927028B2 (en) * 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
DE10154317B4 (en) 2001-10-26 2005-06-09 Epigenomics Ag Method for the detection of cytosine methylations in immobilized DNA samples
US7252935B2 (en) * 2001-11-16 2007-08-07 The John Hopkins University School Of Medicine Method of detection of prostate cancer
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
AU2002360474A1 (en) * 2001-12-03 2003-06-17 Illumina, Inc. Multiplexed methylation detection methods
EP1470254A2 (en) * 2002-01-30 2004-10-27 Epigenomics AG Method for the analysis of cytosine methylation patterns
US6960436B2 (en) * 2002-02-06 2005-11-01 Epigenomics Ag Quantitative methylation detection in DNA samples
WO2003068788A1 (en) * 2002-02-13 2003-08-21 Medimolecular Pty. Ltd. Method of isolating nucleic acids from stool samples
KR100470221B1 (en) * 2002-02-20 2005-02-05 굿젠 주식회사 Minisequencing type oligonucleotide chip for detecting methylation of promoter CpG islands of multiple genes, method for preparing thereof and method for detecting cancer using the same
EP1340818A1 (en) * 2002-02-27 2003-09-03 Epigenomics AG Method and nucleic acids for the analysis of a colon cell proliferative disorder
ATE312946T1 (en) * 2002-03-05 2005-12-15 Epigenomics Ag METHOD AND DEVICE FOR DETERMINING THE TISSUE SPECIFICITY OF FREE DNA IN BODY FLUID
AU2003223041A1 (en) * 2002-04-09 2003-10-20 Epigenomics Ag Method for analysis of methylated nucleic acids
CN100427863C (en) * 2002-06-21 2008-10-22 有限会社日乃出 Device for removing raindrops on umbrella and system for promoting installation of the device
WO2004046313A2 (en) * 2002-06-26 2004-06-03 Cold Spring Harbor Laboratory Methods and compositions for determining methylation profiles
DE10236406C1 (en) 2002-08-02 2003-12-24 Epigenomics Ag Method for the amplification of nucleic acids of low complexity
WO2004013284A2 (en) * 2002-08-02 2004-02-12 Tufts University A method to assess genomic dna methylation using high-performance liquid chromatography-electospray ionization mass spectrometry
AU2003236461B2 (en) * 2002-08-29 2009-05-28 Epigenomics Ag Improved method for bisulfite treatment
WO2004027082A2 (en) * 2002-09-19 2004-04-01 Applera Corporation Methods and compositions for detecting targets
EP2157191B1 (en) * 2002-10-01 2013-12-25 Epigenomics AG Use of PITX2 nucleic acids for the improved treatment of breast cell proliferative disorders
CA2442438C (en) 2002-10-04 2012-06-19 Nisshinbo Industries, Inc. Oligonucleotide-immobilized substrate for detecting methylation
US8039218B2 (en) * 2002-11-14 2011-10-18 John Wayne Cancer Institute Detection of cancer cells in body fluids
AU2003290925A1 (en) * 2002-11-14 2004-06-15 John Wayne Cancer Institute Detection of micro metastasis of melanoma and breast cancer in paraffin-embedded tumor draining lymph nodes by multimaker quantitative rt-pcr
US20040101843A1 (en) * 2002-11-22 2004-05-27 Gerald Zon Detection of methylated DNA sites
WO2004050915A1 (en) * 2002-12-02 2004-06-17 Solexa Limited Determination of methylation of nucleic acid sequences
AU2003900368A0 (en) * 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
ATE373673T1 (en) * 2003-01-29 2007-10-15 Hoffmann La Roche IMPROVED METHOD FOR BISULFITE TREATMENT
DE10304219B3 (en) * 2003-01-30 2004-08-19 Epigenomics Ag Method for the detection of cytosine methylation patterns with high sensitivity
US20090305294A1 (en) * 2003-02-13 2009-12-10 Medimolecular Pty Ltd Method of isolating nucleic acids from stool samples
WO2004087957A2 (en) * 2003-04-03 2004-10-14 Oncomethylome Sciences S.A. Hypermethylated genes and cervical cancer
US20060024676A1 (en) * 2003-04-14 2006-02-02 Karen Uhlmann Method of detecting epigenetic biomarkers by quantitative methyISNP analysis
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
US8150627B2 (en) 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
US8150626B2 (en) * 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
US20050026183A1 (en) * 2003-05-15 2005-02-03 Jian-Bing Fan Methods and compositions for diagnosing conditions associated with specific DNA methylation patterns
ATE476523T1 (en) * 2003-06-17 2010-08-15 Human Genetic Signatures Pty METHOD FOR GENOME AMPLIFICATION
US20080064029A1 (en) * 2003-06-23 2008-03-13 Epigenomics Ag Methods and Nucleic Acids for Analyses of Colorectal Cell Proliferative Disorders
ATE494390T1 (en) * 2003-06-23 2011-01-15 Epigenomics Ag METHOD AND NUCLEIC ACIDS FOR ANALYZING DISORDERS IN COLON CELL PROLIFERATION
ES2546644T3 (en) 2003-06-23 2015-09-25 Epigenomics Ag Methods and nucleic acids for the analysis of proliferative disorders of colorectal cells
US20040265833A1 (en) * 2003-06-23 2004-12-30 Cathy Lofton-Day Methods and nucleic acids for the analysis of colorectal cell proliferative disorders
EP1649050A2 (en) * 2003-06-24 2006-04-26 Epigenomics AG Improved heavymethyl assay for the methylation analysis of the gstpi gene
US20050123944A1 (en) * 2003-08-01 2005-06-09 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
GB0318110D0 (en) * 2003-08-01 2003-09-03 Isaeo Ltd Methods and kits for detecting an enzyme capable of modifying a nucleic acid
DE10338308B4 (en) * 2003-08-15 2006-10-19 Epigenomics Ag Method for the detection of cytosine methylations in DNA
US7262013B2 (en) * 2003-08-29 2007-08-28 Applera Corporation Bisulfite method
US20050069895A1 (en) * 2003-08-29 2005-03-31 Applera Corporation Compositions, methods, and kits for fabricating coded molecular tags
US7534873B2 (en) * 2003-08-29 2009-05-19 Applied Biosystems, Llc Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
US7198900B2 (en) * 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US20050048498A1 (en) * 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
US7371526B2 (en) 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
US7368239B2 (en) 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
JP4714148B2 (en) * 2003-09-04 2011-06-29 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド Nucleic acid detection assay
CA2541706C (en) * 2003-10-08 2014-02-18 The Trustees Of Boston University Methods for prenatal diagnosis of chromosomal abnormalities
KR20110138418A (en) 2003-10-09 2011-12-27 에피제노믹스 아게 Improved bisulfite conversion of dna
EP2339024B1 (en) * 2003-10-21 2014-12-31 Orion Genomics, LLC Methods for quantitative determination of methylation density in a DNA locus
WO2005054517A2 (en) 2003-12-01 2005-06-16 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with the development of prostate cell proliferative disorders
CA2547743C (en) 2003-12-02 2011-06-28 F. Hoffmann-La Roche Ag Improved method for bisulfite treatment
EP1561821B1 (en) 2003-12-11 2011-02-16 Epigenomics AG Prognostic markers for prediction of treatment response and/or survival of breast cell proliferative disorder patients
AU2004237861B2 (en) * 2003-12-11 2010-04-29 Epigenomics Ag Prognostic markers for prediction of treatment response and/or survival of breast cell proliferative disorder patients
WO2005076837A2 (en) * 2004-02-10 2005-08-25 Cornell Research Foundation, Inc. Method for detection of promoter methylation status
US7459274B2 (en) * 2004-03-02 2008-12-02 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification
EP1730312B1 (en) * 2004-03-24 2008-07-02 Applera Corporation Encoding and decoding reactions for determining target polynucleotides
EP1655377A1 (en) * 2004-04-06 2006-05-10 Epigenomics AG Method of quantifying methylated DNA
US20050287553A1 (en) * 2004-04-06 2005-12-29 Epigenomics Ag Method for the quantification of methylated DNA
US20050233340A1 (en) * 2004-04-20 2005-10-20 Barrett Michael T Methods and compositions for assessing CpG methylation
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
WO2006076017A2 (en) * 2004-04-30 2006-07-20 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US7364855B2 (en) * 2004-04-30 2008-04-29 Applera Corporation Methods and kits for methylation detection
JPWO2005116254A1 (en) * 2004-05-25 2008-04-03 財団法人ヒューマンサイエンス振興財団 Method for quantitatively evaluating DNA methylation
EP1609873B1 (en) * 2004-06-23 2010-12-08 Epigenomics AG Method for quantification of methylated DNA
US7943308B2 (en) * 2004-06-23 2011-05-17 Epigenomics Ag Methods and nucleic acids for the detection of metastasis of colon cell proliferative disorders
US20060234252A1 (en) * 2004-07-02 2006-10-19 Applera Corporation Methods and kits for methylation detection
ATE408032T1 (en) * 2004-07-16 2008-09-15 Oncomethylome Sciences Sa ESR1 AND CERVICAL CANCER
WO2006008128A2 (en) 2004-07-18 2006-01-26 Epigenomics Ag Epigenetic methods and nucleic acids for the detection of breast cell proliferative disorders
EP1632578A1 (en) 2004-09-03 2006-03-08 Roche Diagnostics GmbH DNA decontamination method
JP4980219B2 (en) * 2004-09-10 2012-07-18 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド Amplification blocker comprising intercalating nucleic acid (INA) containing intercalated pseudonucleotide (IPN)
WO2006034009A2 (en) * 2004-09-20 2006-03-30 Sigma-Aldrich Co. Purification of biomolecules from contaminating intact nucleic acids
EP1812589A2 (en) * 2004-09-30 2007-08-01 Epigenomics AG Epigenetic methods and nucleic acids for the detection of lung cell proliferative disorders
US7658288B2 (en) * 2004-11-08 2010-02-09 Applied Biosystems, Llc Bisulfite conversion reagent
EP1815026A2 (en) 2004-11-10 2007-08-08 University Of Southern California High throughput methods comprising analysis of repetitive element dna methylation
CA2593546A1 (en) 2004-12-02 2006-07-06 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with the prognosis of prostate cell proliferative disorders
WO2006058393A1 (en) * 2004-12-03 2006-06-08 Human Genetic Signatures Pty Ltd Methods for simplifying microbial nucleic acids by chemical modification of cytosines
EP1829978B1 (en) 2004-12-13 2011-09-21 Bio-Dixam LLC Method of detecting gene methylation and method of examining neoplasm by detecting methylation
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
EP1828414A4 (en) * 2004-12-23 2008-12-10 Human Genetic Signatures Pty Detection of human papilloma virus
WO2006088940A2 (en) * 2005-02-14 2006-08-24 The Johns Hopkins University Neoplasia screening compositions and methods of use
US20060188910A1 (en) * 2005-02-18 2006-08-24 Applera Corporation Compositions, methods, and kits for analyzing DNA methylation
WO2006103111A2 (en) 2005-04-01 2006-10-05 Epigenomics Ag Improved bisulfite conversion of dna
US7749702B2 (en) * 2005-04-15 2010-07-06 Epigenomics Ag Methods and nucleic acids for the analyses of cellular proliferative disorders
WO2006119434A2 (en) * 2005-05-02 2006-11-09 University Of Southern California DNA METHYLATION MARKERS ASSOCIATED WITH THE CpG ISLAND METHYLATOR PHENOTYPE (CIMP) IN HUMAN COLORECTAL CANCER
KR100673811B1 (en) * 2005-05-11 2007-01-24 한국표준과학연구원 Method for quantitative analysis of methyl cytosine in DNA and uses thereof
EP1883707B1 (en) * 2005-05-26 2014-04-09 Human Genetic Signatures PTY Ltd Isothermal strand displacement amplification using primers containing a non-regular base
WO2006131391A1 (en) * 2005-06-10 2006-12-14 Epigenomics Ag Prognostic assay for prediction of treatment response and/or survival of breast cell proliferative disorder patients
US20070037184A1 (en) * 2005-06-16 2007-02-15 Applera Corporation Methods and kits for evaluating dna methylation
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US20090005268A1 (en) * 2005-07-18 2009-01-01 Epigenomics Ag Compositions and Methods for Cancer Diagnostics Comprising Pan-Cancer Markers
ATE452993T1 (en) * 2005-07-21 2010-01-15 Epigenomics Ag METHOD FOR QUANTIFYING METHYLATED DNA
EP1943353B1 (en) * 2005-09-14 2011-11-02 Human Genetic Signatures PTY Ltd Assay for a health state
WO2007039128A1 (en) 2005-09-21 2007-04-12 Epigenomics Ag Markers for the prediction of outcome of anthracycline treatment
US20090191548A1 (en) 2005-09-29 2009-07-30 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with tissue classification
US20080254470A1 (en) * 2005-10-03 2008-10-16 Epigenomics Ag Methods and Nucleic Acids For the Analysis of Gene Expression Associated With the Prognosis of Cell Proliferative Disorders
US20090042195A1 (en) * 2005-10-07 2009-02-12 Bradford Coffee Methods and systems for screening for and diagnosing dna methylation associated abnormalities and sex chromosome aneuploidies
US7770717B2 (en) * 2005-10-12 2010-08-10 Scanvaegt International A/S Device for transfer of items
US20070087358A1 (en) * 2005-10-19 2007-04-19 Melanie Ehrlich Methods for diagnosing cancer based on DNA methylation status in NBL2
EP2186912A3 (en) 2005-11-08 2011-01-19 Euclid Diagnostics LLC Materials and methods for assaying for methylation of CpG islands associated with genes in the evaluation of cancer
EP1969139A1 (en) 2005-11-17 2008-09-17 Epigenomics AG Method for the determination of the dna methylation level of a cpg position in identical cells within a tissue sample
DE602006016859D1 (en) * 2005-12-14 2010-10-21 Roche Diagnostics Gmbh NEW PROCESS FOR BISULFIT TREATMENT
CA2629824A1 (en) * 2005-12-16 2007-06-21 University Of Maryland, Baltimore Methylation of gene promoters as a predictor of effectiveness of therapy
DE102006001161A1 (en) 2006-01-06 2007-07-12 Qiagen Gmbh Method for the detection of cytosine methylations
WO2007088744A1 (en) * 2006-02-03 2007-08-09 The University Of Tokyo Method for cell identification based on methylation state of genomic dna
US7820385B2 (en) * 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA
EP1840224A1 (en) 2006-03-29 2007-10-03 Pangaea Biotech, S.A. Method of predicting survival of a non-small-cell lung cancer patient to a chemotherapeutic treatment
US20090111120A1 (en) * 2006-03-30 2009-04-30 University Of Maryland, Baltimore Methylation of genes as a predictor of polyp formation and recurrence
US7901882B2 (en) * 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
EP2484778A3 (en) 2006-04-17 2012-10-10 Epigenomics AG Methods and nucleic acids for the detection of colorectal cell proliferative disorders
US20090317810A1 (en) * 2006-04-17 2009-12-24 Epigenomics Ag Methods and nucleic acids for the detection of colorectal cell proliferative disorders
US20080003609A1 (en) * 2006-05-10 2008-01-03 The Cleveland Clinic Foundation Method of detecting bladder urothelial carcinoma
KR100761031B1 (en) 2006-05-27 2007-10-04 김연수 Cancer Detection Kit Using the DNA Methylation
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
CA2657576C (en) 2006-07-14 2023-10-31 The Regents Of The University Of California Cancer biomarkers and methods of use thereof
ES2597845T3 (en) * 2006-07-21 2017-01-23 Epigenomics Ag Methods for the analysis of cell proliferative disorders
ES2599814T3 (en) 2006-07-21 2017-02-03 Epigenomics Ag Methods and nucleic acids related to the GLI3 gene for analysis of cell proliferative disorders
AU2007283143A1 (en) * 2006-08-08 2008-02-14 Epigenomics Ag A method for methylation analysis of nucleic acid
US20080124735A1 (en) * 2006-10-16 2008-05-29 Matthias Schuster Method for detection of one or more CpG positions
ATE535621T1 (en) 2006-10-18 2011-12-15 Epigenomics Ag MOLECULE FOR PROVIDING A STANDARD FOR THE QUANTITATIVE ANALYSIS OF THE METHYLATION STATUS OF A NUCLEIC ACID
JP5562034B2 (en) 2006-11-24 2014-07-30 エピゲノミクス アーゲー Method and nucleic acid for analyzing gene expression related to the development of prostate cell proliferative disorder
JP2008136404A (en) * 2006-11-30 2008-06-19 Sysmex Corp Method for confirming amount of dna after conversion treatment of non-methylated cytosine in dna methylation detection
US20090203011A1 (en) * 2007-01-19 2009-08-13 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
ATE549418T1 (en) 2007-01-19 2012-03-15 Epigenomics Ag METHOD AND NUCLEIC ACIDS FOR ANALYZING PROLIFERATIVE CELL DISEASES
US8206927B2 (en) * 2007-01-23 2012-06-26 Sequenom, Inc. Method for accurate assessment of DNA quality after bisulfite treatment
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
EP2126120A4 (en) * 2007-03-16 2011-03-30 Human Genetic Signatures Pty Assay for gene expression
WO2008128198A2 (en) * 2007-04-12 2008-10-23 Usc Stevens - University Of Southern California Dna methylation analysis by digital bisulfite genomic sequencing and digital methylight
US20100210700A1 (en) 2007-05-08 2010-08-19 Schering Corporation Methods of treatment using intravenous formulations comprising temozolomide
WO2009006543A1 (en) 2007-07-02 2009-01-08 Euclid Diagnostics Llc Methods for evaluating the methylation status of a polynucleotide
US20090042290A1 (en) * 2007-08-06 2009-02-12 Christopher Steele Method of modifying a macromolecule without prior extraction from a sample
CN101932723B (en) * 2007-09-17 2014-09-10 肿瘤甲基化科学公司 Improved detection of mage-a expression
WO2009059327A2 (en) * 2007-11-02 2009-05-07 University Of Southern California (Usc) Compositions and methods comprising biomarkers of sperm quality, semen quality and fertility
AU2008329549B2 (en) * 2007-11-27 2013-08-22 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
WO2009074328A2 (en) 2007-12-11 2009-06-18 Epigenomics Ag Methods and nucleic acids for analyses of lung carcinoma
CA2709632A1 (en) * 2007-12-20 2009-07-02 Human Genetic Signatures Pty Ltd Elimination of contaminants associated with nucleic acid amplification
DE102008008313A1 (en) * 2008-02-07 2009-08-13 Qiagen Gmbh Amplification of bisulfited nucleic acids
EP2294216A4 (en) 2008-05-14 2011-11-23 Dermtech Int Diagnosis of melanoma and solar lentigo by nucleic acid analysis
EP2304057B1 (en) 2008-06-17 2014-09-17 Signature Diagnostics AG Method for the detection of ovarian cancer
US20100021902A1 (en) * 2008-06-27 2010-01-28 Dimo Dietrich Method for methylation-selective amplification
US20100120034A1 (en) * 2008-07-03 2010-05-13 Life Technologies Corporation Methylation analysis of mate pairs
US8361746B2 (en) 2008-07-24 2013-01-29 Brookhaven Science Associates, Llc Methods for detection of methyl-CpG dinucleotides
EP2340314B8 (en) 2008-10-22 2015-02-18 Illumina, Inc. Preservation of information related to genomic dna methylation
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
SG171916A1 (en) * 2008-12-02 2011-07-28 Bio Rad Laboratories Chromatin structure detection
US8497066B2 (en) * 2008-12-04 2013-07-30 Rush University Medical Center DNA methylation based test for monitoring efficacy of treatment
WO2010083046A2 (en) * 2009-01-15 2010-07-22 The Salk Institute For Biological Studies Methods for using next generation sequencing to identify 5-methyl cytosines in the genome
EP2966181B1 (en) 2009-06-26 2018-02-14 Epigenomics AG Methods and nucleic acids for analysis of bladder carcinoma
US9624530B2 (en) 2009-08-03 2017-04-18 Epigenomics Ag Methods for preservation of genomic DNA sequence complexity
EP2287335A1 (en) * 2009-08-05 2011-02-23 DiaSorin S.p.A. Highly sensitive rapid isothermal method for the detection of methylation patterns
US20110046009A1 (en) * 2009-08-24 2011-02-24 Perkinelmer Health Sciences, Inc. Methods for detecting dna methylation using encoded particles
WO2011051414A1 (en) 2009-10-28 2011-05-05 Signature Diagnostics Ag Method for the prognosis of ovarian carcinoma
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
US8568984B2 (en) * 2010-02-19 2013-10-29 Philadelphia Health & Education Corporation Methods of diagnosing non-urinary tract diseases by detecting aberrant methylation
US20120003639A1 (en) 2010-04-27 2012-01-05 Prelude, Inc. Cancer biomarkers and methods of use thereof
ES2581594T3 (en) * 2010-05-25 2016-09-06 The Johns Hopkins University Compositions and methods to detect a neoplasm
US9273347B2 (en) 2010-09-10 2016-03-01 Bio-Rad Laboratories, Inc. Detection of RNA-interacting regions in DNA
BR112013005929B1 (en) 2010-09-13 2021-02-09 Clinical Genomics Pty. Ltd. methods of screening for onset or predisposition to onset of large intestine neoplasm or monitoring of neoplasm progress in an individual
US10428390B2 (en) * 2010-12-16 2019-10-01 Genomictree, Inc. Method for detecting methylation of colorectal cancer specific methylation marker gene for colorectal cancer diagnosis
AU2012214312A1 (en) 2011-02-09 2013-08-22 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
WO2012112606A1 (en) 2011-02-15 2012-08-23 Bio-Rad Laboratories, Inc. Detecting methylati0n in a subpopulation of genomic dna
US10435743B2 (en) 2011-05-20 2019-10-08 The Regents Of The University Of California Method to estimate age of individual based on epigenetic markers in biological sample
ES2654561T3 (en) 2011-07-08 2018-02-14 Epigenomics Ag Procedures and nucleic acids to determine the prognosis of a subject with cancer
US8728987B2 (en) 2011-08-03 2014-05-20 Bio-Rad Laboratories, Inc. Filtering small nucleic acids using permeabilized cells
EP2748333B1 (en) 2011-08-25 2017-11-22 Clinical Genomics Pty Ltd Dna methylation in colorectal cancer diagnostic methods
KR20140057356A (en) 2011-08-31 2014-05-12 제넨테크, 인크. Diagnostic markers
MY167564A (en) 2011-09-07 2018-09-14 Human Genetic Signatures Pty Ltd Molecular detection assay
WO2013055530A1 (en) 2011-09-30 2013-04-18 Genentech, Inc. Diagnostic methylation markers of epithelial or mesenchymal phenotype and response to egfr kinase inhibitor in tumours or tumour cells
US10131953B2 (en) 2011-11-03 2018-11-20 Wisconsin Alumni Research Foundation Unbiased DNA methylation markers define an extensive field defect in histologically normal prostate tissues associated with prostate cancer: new biomarkers for men with prostate cancer
WO2013084075A2 (en) 2011-12-06 2013-06-13 Mdxhealth Sa Methods of detecting mutations and epigenetic changes
WO2013096661A1 (en) 2011-12-22 2013-06-27 Illumina, Inc. Methylation biomarkers for ovarian cancer
EP3524693A1 (en) 2012-04-30 2019-08-14 Raindance Technologies, Inc. Digital analyte analysis
ES2749514T3 (en) 2012-05-11 2020-03-20 Clinical Genomics Pty Ltd Colorectal Cancer Diagnostic Genetic Markers Panel
WO2013170191A1 (en) 2012-05-11 2013-11-14 Genentech, Inc. Methods of using antagonists of nad biosynthesis from nicotinamide
EP2850212B1 (en) 2012-05-18 2020-01-01 Clinical Genomics Pty. Ltd. A method of screening for colorectal cancer
US10233503B2 (en) 2012-05-24 2019-03-19 Fundació Institut D'investigació Biomèdica De Bellvitge (Idibell) Method for the identification of the origin of a cancer of unknown primary origin by methylation analysis
EP2891719A4 (en) * 2012-08-28 2016-06-15 Istat Biomedical Co Ltd Test composition for screening cancers
WO2014071281A1 (en) 2012-11-02 2014-05-08 The Johns Hopkins University Dna methylation biomarkers of post-partum depression risk
US9427737B2 (en) 2012-12-14 2016-08-30 Bio-Rad Laboratories, Inc. Methods and compositions for using oils for analysis and detection of molecules
US20140242583A1 (en) * 2013-02-28 2014-08-28 Jim Z. Lu Assays, methods and compositions for diagnosing cancer
WO2014160117A1 (en) * 2013-03-14 2014-10-02 Abbott Molecular Inc. Multiplex methylation-specific amplification systems and methods
US20140274757A1 (en) 2013-03-14 2014-09-18 Marie K. Kirby Differential Methylation Level of CpG Loci That Are Determinative of a Biochemical Reoccurrence of Prostate Cancer
US9758835B2 (en) 2013-03-15 2017-09-12 Baylor Research Institute Ulcerative colitis (UC)-associated colorectal neoplasia markers
ES2820732T3 (en) 2013-03-28 2021-04-22 Wisconsin Alumni Res Found Unbiased DNA Methylation Markers Defining a Wide-Field Defect in Histologically Normal Prostate Tissues Associated with Prostate Cancer: New Biomarkers for Men with Prostate Cancer
EP2986762B1 (en) 2013-04-19 2019-11-06 Bio-Rad Laboratories, Inc. Digital analyte analysis
CN104250663B (en) * 2013-06-27 2017-09-15 北京大学 The high-flux sequence detection method on methylated CpG island
AU2014287090B2 (en) 2013-07-11 2020-02-06 The Johns Hopkins University A DNA methylation and genotype specific biomarker of suicide attempt and/or suicide ideation
GB201402644D0 (en) * 2014-02-14 2014-04-02 Base4 Innovation Ltd Methylation detection method
MY193347A (en) 2014-05-02 2022-10-06 Malaysian Palm Oil Board Mantle phenotype detection in palm
WO2015169947A1 (en) 2014-05-09 2015-11-12 Lifecodexx Ag Detection of dna that originates from a specific cell-type and related methods
EP2942400A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Multiplex detection of DNA that originates from a specific cell-type
EP2942401A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Detection of DNA that originates from a specific cell-type
WO2015181804A2 (en) * 2014-05-30 2015-12-03 Pontificia Universidad Católica De Chile Method and assay for the non-invasive detection of early gastric cancer by the combined use of methylated reprimo cell-free dna and pepsinogen i/ii
WO2015184498A2 (en) 2014-06-05 2015-12-10 Clinical Genomics Pty Ltd Method for methylation analysis
WO2016057485A1 (en) 2014-10-06 2016-04-14 The Johns Hopkins University A dna methylation and genotype specific biomarker for predicting post-traumatic stress disorder
WO2016061465A1 (en) * 2014-10-17 2016-04-21 The Regents Of The University Of Colorado, A Body Corporate Biomarkers for head and neck cancer and methods of their use
EP3227687A4 (en) 2014-12-05 2018-10-24 Prelude, Inc. Dcis recurrence and invasive breast cancer
US9984201B2 (en) 2015-01-18 2018-05-29 Youhealth Biotech, Limited Method and system for determining cancer status
HUE050491T2 (en) 2015-11-10 2020-12-28 Eurofins Lifecodexx Gmbh Detection of foetal chromosomal aneuploidies using dna regions that are differentially methylated between the foetus and the pregnant female
SG10201912825XA (en) 2015-11-27 2020-02-27 Cartherics Pty Ltd Genetically modified cells and uses thereof
CA3168463A1 (en) 2015-12-17 2017-06-22 Illumina, Inc. Distinguishing methylation levels in complex biological samples
US10913986B2 (en) 2016-02-01 2021-02-09 The Board Of Regents Of The University Of Nebraska Method of identifying important methylome features and use thereof
US10202650B2 (en) 2016-05-31 2019-02-12 Youhealth Biotech, Limited Methods for monitoring ELOVL2, KLF14 and PENK gene expression following treatment with vitamin C
US10093986B2 (en) 2016-07-06 2018-10-09 Youhealth Biotech, Limited Leukemia methylation markers and uses thereof
WO2018009707A1 (en) 2016-07-06 2018-01-11 Youhealth Biotech, Limited Solid tumor methylation markers and uses thereof
US11396678B2 (en) 2016-07-06 2022-07-26 The Regent Of The University Of California Breast and ovarian cancer methylation markers and uses thereof
US11130998B2 (en) 2016-11-14 2021-09-28 Wisconsin Alumni Research Foundation Unbiased DNA methylation markers define an extensive field defect in histologically normal prostate tissues associated with prostate cancer: new biomarkers for men with prostate cancer
HUE059194T2 (en) 2016-12-16 2022-10-28 Eurofins Genomics Europe Sequencing GmbH Epigenetic markers and related methods and means for the detection and management of ovarian cancer
US10513739B2 (en) 2017-03-02 2019-12-24 Youhealth Oncotech, Limited Methylation markers for diagnosing hepatocellular carcinoma and lung cancer
EP3655418A4 (en) 2017-06-22 2021-05-19 Triact Therapeutics, Inc. Methods of treating glioblastoma
WO2019067991A1 (en) 2017-09-29 2019-04-04 Triact Therapeutics, Inc. Iniparib formulations and uses thereof
JP2022500504A (en) 2018-09-14 2022-01-04 プレリュード コーポレーションPrelude Corporation How to choose treatment for subjects at risk of invasive breast cancer
EP3911761A4 (en) 2019-01-18 2022-10-19 The Regents of the University of California Dna methylation measurement for mammals based on conserved loci
WO2020198229A1 (en) 2019-03-26 2020-10-01 Dermtech, Inc. Novel gene classifiers and uses thereof in skin cancers
US11542548B2 (en) 2019-06-11 2023-01-03 Wisconsin Alumni Research Foundation; Blood DNA methylation biomarker diagnostic test for anxiety and depressive disorders
CN112662761A (en) * 2020-03-05 2021-04-16 博尔诚（北京）科技有限公司 Probe composition for detecting 3 parenchymal organ tumors
EP4150119A1 (en) 2020-05-22 2023-03-22 Wisconsin Alumni Research Foundation Epigenetic modification of select genes as markers of hypersomnolence
WO2023052640A1 (en) 2021-09-30 2023-04-06 Tivenix Sa A method for diagnosing and predicting progression of neurodegenerative diseases or disorders
EP4170661A1 (en) 2021-10-19 2023-04-26 Institut National de la Santé et de la Recherche Médicale (INSERM) Methylation profile analysis using smoothing method
WO2023066972A1 (en) 2021-10-19 2023-04-27 INSERM (Institut National de la Santé et de la Recherche Médicale) Dna methylation signature for diagnosing hepatocellular carcinoma
WO2023175019A1 (en) 2022-03-15 2023-09-21 Genknowme S.A. Method determining the difference between the biological age and the chronological age of a subject
WO2024044578A1 (en) 2022-08-22 2024-02-29 University Of Virginia Dna methylation biomarkers of premenstrual dysphoric disorder and perimenopausal depression
WO2024059649A1 (en) 2022-09-13 2024-03-21 The Saban Research Institute; Children's Hospital Los Angeles Dna methylation signature of retinoblastoma

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5788146A (en) * 1996-02-13 1998-08-04 Bradford Company Parent welding partition assembly
GB9609441D0 (en) * 1996-05-04 1996-07-10 Zeneca Ltd Process
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US5736333A (en) * 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
EP0912766B2 (en) 1996-06-04 2011-12-14 University of Utah Research Foundation Monitoring hybridization during pcr
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
AUPP312998A0 (en) 1998-04-23 1998-05-14 Commonwealth Scientific And Industrial Research Organisation Diagnostic assay
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US6472156B1 (en) * 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
AU2004237861B2 (en) 2003-12-11 2010-04-29 Epigenomics Ag Prognostic markers for prediction of treatment response and/or survival of breast cell proliferative disorder patients
US20050287553A1 (en) 2004-04-06 2005-12-29 Epigenomics Ag Method for the quantification of methylated DNA
CA2593546A1 (en) 2004-12-02 2006-07-06 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with the prognosis of prostate cell proliferative disorders

Also Published As

Publication number Publication date
DE60029323T3 (en) 2017-03-30
EP1710321B1 (en) 2015-12-09
EP1185695A4 (en) 2004-05-19
US8323890B2 (en) 2012-12-04
US20070212699A1 (en) 2007-09-13
ES2564048T3 (en) 2016-03-17
DK1185695T4 (en) 2017-02-20
WO2000070090A1 (en) 2000-11-23
US20100009365A1 (en) 2010-01-14
US7112404B2 (en) 2006-09-26
EP1710321A3 (en) 2007-04-04
AU2004229115B2 (en) 2007-08-23
US6331393B1 (en) 2001-12-18
AU2004229115A1 (en) 2004-12-09
DK1710321T3 (en) 2016-03-07
CA2372665C (en) 2007-07-17
EP1185695A1 (en) 2002-03-13
US7553627B2 (en) 2009-06-30
AU775798B2 (en) 2004-08-19
US20020086324A1 (en) 2002-07-04
JP4044290B2 (en) 2008-02-06
EP1185695B2 (en) 2016-11-09
EP1185695B1 (en) 2006-07-12
CY1118000T1 (en) 2017-05-17
DE60029323T2 (en) 2007-07-05
DK1185695T3 (en) 2006-11-06
JP2007068539A (en) 2007-03-22
JP2002543852A (en) 2002-12-24
EP1710321A2 (en) 2006-10-11
ATE332980T2 (en) 2006-08-15
ES2267535T3 (en) 2007-03-16
DE60029323D1 (en) 2006-08-24
ES2267535T5 (en) 2017-05-30
AU4712200A (en) 2000-12-05

Similar Documents

Publication Publication Date Title
CA2372665A1 (en) Process for high throughput dna methylation analysis
Stewart et al. oxBS-450K: a method for analysing hydroxymethylation using 450K BeadChips
ES2382746T3 (en) Method to determine DNA methylation in blood or urine samples
EP1590362B2 (en) Improved method for bisulfite treatment
IL154663A (en) Method for determining the degree of methylation of defined cytosines in genomic dna in the sequence context 5'-cpg-3'
Qi et al. Analytical methods for locating modifications in nucleic acids
CA2321185A1 (en) Method for detection of target nucleic acids using pcr
WO2004099431A3 (en) Nucleic acid sequence detection using multiplexed oligonucleotide pcr
CN103233072B (en) High-flux mythelation detection technology for DNA (deoxyribonucleic acid) of complete genome
WO2002072880A3 (en) Method for detecting cytosine methylation patterns having high sensitivity
WO2001075166A3 (en) Compositions and methods for detecting and quantifying gene expression
US20100081145A1 (en) Detecting Prostate Cancer
WO2002086163A8 (en) Methods for high throughput genome analysis using restriction site tagged microarrays
JP2002538839A (en) Probe / mobility modifier complex for multiplex nucleic acid detection
Khodadadi et al. Current advances in DNA methylation analysis methods
CN110129436A (en) The Serial No. of DNA methylation is analyzed
JP4435174B2 (en) Improved method of bisulfite treatment.
Sauer Typing of single nucleotide polymorphisms by MALDI mass spectrometry: principles and diagnostic applications
US20090004646A1 (en) Method for Quantifying Methylated Dna
WO2002044416A3 (en) Sulfonated diarylrhodamine dyes as fluorescent labels
WO2005001142A3 (en) Methods and nucleic acids for the analysis of colorectal cell proliferative disorders
WO2011063210A2 (en) Methods of mapping genomic methylation patterns
WO2003074739A3 (en) Automated allele determination using fluorometric genotyping
US20080286778A1 (en) Method for Investigating Cytosine Methylations in Dna
US20080172183A1 (en) Systems and methods for methylation prediction

Legal Events

Date Code Title Description
EEER Examination request