CA2372665A1 - Process for high throughput dna methylation analysis - Google Patents

Process for high throughput dna methylation analysis Download PDF

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Publication number
CA2372665A1
CA2372665A1 CA002372665A CA2372665A CA2372665A1 CA 2372665 A1 CA2372665 A1 CA 2372665A1 CA 002372665 A CA002372665 A CA 002372665A CA 2372665 A CA2372665 A CA 2372665A CA 2372665 A1 CA2372665 A1 CA 2372665A1
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CA
Canada
Prior art keywords
nucleic acid
genomic dna
high throughput
dna samples
specifically
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CA002372665A
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French (fr)
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CA2372665C (en
Inventor
Peter W. Laird
Cindy A. Eads
Kathleen D. Danenberg
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University of Southern California USC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

There is disclosed an improved high-throughput and quantitative process for determining methylation patterns in genomic DNA samples based on amplifying modified nucleic acid, and detecting methylated nucleic acid based on amplification-dependent displacement of specifically annealed hybridization probes. Specifically, the inventive process provides for treating genomic DNA samples with sodium bisulfite to create methylation-dependent sequence differences, followed by detection with fluorescence-based quantitative PCR
techniques. The process is particularly well suited for the rapid analysis of a large number of nucleic acid samples, such as those from collections of tumor tissues.
CA002372665A 1999-05-14 2000-05-12 Process for high throughput dna methylation analysis Expired - Lifetime CA2372665C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/311,912 US6331393B1 (en) 1999-05-14 1999-05-14 Process for high-throughput DNA methylation analysis
US09/311,912 1999-05-14
PCT/US2000/013029 WO2000070090A1 (en) 1999-05-14 2000-05-12 Process for high throughput dna methylation analysis

Publications (2)

Publication Number Publication Date
CA2372665A1 true CA2372665A1 (en) 2000-11-23
CA2372665C CA2372665C (en) 2007-07-17

Family

ID=23209034

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CA002372665A Expired - Lifetime CA2372665C (en) 1999-05-14 2000-05-12 Process for high throughput dna methylation analysis

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US (4) US6331393B1 (en)
EP (2) EP1185695B2 (en)
JP (2) JP4044290B2 (en)
AT (1) ATE332980T2 (en)
AU (2) AU775798B2 (en)
CA (1) CA2372665C (en)
CY (1) CY1118000T1 (en)
DE (1) DE60029323T3 (en)
DK (2) DK1185695T4 (en)
ES (2) ES2267535T5 (en)
WO (1) WO2000070090A1 (en)

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* Cited by examiner, † Cited by third party
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