CA2370535A1 - Replica amplification of nucleic acid arrays - Google Patents

Replica amplification of nucleic acid arrays Download PDF

Info

Publication number
CA2370535A1
CA2370535A1 CA002370535A CA2370535A CA2370535A1 CA 2370535 A1 CA2370535 A1 CA 2370535A1 CA 002370535 A CA002370535 A CA 002370535A CA 2370535 A CA2370535 A CA 2370535A CA 2370535 A1 CA2370535 A1 CA 2370535A1
Authority
CA
Canada
Prior art keywords
arrays
methods
replicas
immobilized
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002370535A
Other languages
French (fr)
Other versions
CA2370535C (en
Inventor
George M. Church
Robi D. Mitra
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard College
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA002582809A priority Critical patent/CA2582809A1/en
Priority to CA002411514A priority patent/CA2411514A1/en
Publication of CA2370535A1 publication Critical patent/CA2370535A1/en
Application granted granted Critical
Publication of CA2370535C publication Critical patent/CA2370535C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00641—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being continuous, e.g. porous oxide substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00644—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being present in discrete locations, e.g. gel pads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00657—One-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays.
Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.
CA002370535A 1999-03-12 2000-03-10 Replica amplification of nucleic acid arrays Expired - Lifetime CA2370535C (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002582809A CA2582809A1 (en) 1999-03-12 2000-03-10 Replica amplification of nucleic acid arrays
CA002411514A CA2411514A1 (en) 1999-03-12 2000-03-10 Nucleotide compounds having a cleavable linker

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/267,496 1999-03-12
US09/267,496 US6485944B1 (en) 1997-10-10 1999-03-12 Replica amplification of nucleic acid arrays
PCT/US2000/006390 WO2000053812A2 (en) 1999-03-12 2000-03-10 Replica amplification of nucleic acid arrays

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CA002582809A Division CA2582809A1 (en) 1999-03-12 2000-03-10 Replica amplification of nucleic acid arrays
CA002411514A Division CA2411514A1 (en) 1999-03-12 2000-03-10 Nucleotide compounds having a cleavable linker

Publications (2)

Publication Number Publication Date
CA2370535A1 true CA2370535A1 (en) 2000-09-14
CA2370535C CA2370535C (en) 2008-05-13

Family

ID=23019033

Family Applications (3)

Application Number Title Priority Date Filing Date
CA002582809A Abandoned CA2582809A1 (en) 1999-03-12 2000-03-10 Replica amplification of nucleic acid arrays
CA002411514A Abandoned CA2411514A1 (en) 1999-03-12 2000-03-10 Nucleotide compounds having a cleavable linker
CA002370535A Expired - Lifetime CA2370535C (en) 1999-03-12 2000-03-10 Replica amplification of nucleic acid arrays

Family Applications Before (2)

Application Number Title Priority Date Filing Date
CA002582809A Abandoned CA2582809A1 (en) 1999-03-12 2000-03-10 Replica amplification of nucleic acid arrays
CA002411514A Abandoned CA2411514A1 (en) 1999-03-12 2000-03-10 Nucleotide compounds having a cleavable linker

Country Status (6)

Country Link
US (3) US6485944B1 (en)
EP (2) EP1235929A2 (en)
JP (1) JP2003526331A (en)
AU (1) AU3876100A (en)
CA (3) CA2582809A1 (en)
WO (1) WO2000053812A2 (en)

Families Citing this family (325)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998035012A2 (en) 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
EP1165235B1 (en) 1999-03-19 2011-09-28 Life Technologies Corporation Method of screening mutated cells
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US8137906B2 (en) * 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
GB2360282A (en) * 2000-03-17 2001-09-19 Bioinvent Int Ab Making and using micro-arrays of biological materials
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
ATE356222T1 (en) 2000-10-06 2007-03-15 Univ Columbia MASSIVE PARALLEL METHOD FOR DECODING DNA AND RNA
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
DE60039792D1 (en) * 2000-10-23 2008-09-18 Ingeny Holding Bv METHOD AND DEVICE FOR DETECTING MUTATIONS IN NUCLEIC ACID SAMPLES
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AU2002228974A1 (en) 2000-12-13 2002-06-24 Nugen Technologies, Inc Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
AU2002233540A1 (en) * 2001-02-23 2002-09-12 Pyrosequencing Ab Allele-specific primer extension assay
MXPA02012739A (en) 2001-03-09 2004-04-20 Nugen Technologies Inc Methods and compositions for amplification of rna sequences.
US9777312B2 (en) * 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US7668697B2 (en) 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
EP1415001A4 (en) 2001-07-13 2008-02-20 Ambergen Inc Nucleotide compositions comprising photocleavable markers and methods of preparation thereof
DK1314783T3 (en) * 2001-11-22 2009-03-16 Sloning Biotechnology Gmbh Nucleic acid linkers and their use in gene synthesis
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
ATE304610T1 (en) * 2002-04-04 2005-09-15 Biotage Ab PRIMER EXTENSION BASED METHOD USING NUCLEOTIDES LABELED BY CLIVABLE LINKERS
US7452712B2 (en) 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
SI3147292T1 (en) 2002-08-23 2019-01-31 Illumina Cambridge Limited Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
WO2004029294A1 (en) * 2002-09-27 2004-04-08 Biotage Ab New sequencing method for sequencing rna molecules
JP4329322B2 (en) * 2002-10-04 2009-09-09 ソニー株式会社 INTERACTIVE ACTION DETECTION METHOD, BIO ASSAY DEVICE, AND BIO ASSY
WO2004050839A2 (en) 2002-11-27 2004-06-17 Sequenom, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
US8323897B2 (en) 2002-12-04 2012-12-04 Applied Biosystems, Llc Multiplex amplification of polynucleotides
EP1608952B1 (en) 2002-12-20 2016-08-10 Life Technologies Corporation Assay apparatus and method using microfluidic arrays
EP1613778B1 (en) 2003-04-14 2014-11-26 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
DE602004018631D1 (en) 2003-04-24 2009-02-05 Afshin Ahmadian EN
US20050039420A1 (en) * 2003-07-31 2005-02-24 Albritton Charles Wade Fluid sensing in a drip tray
US9394565B2 (en) 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
WO2005029041A2 (en) * 2003-09-19 2005-03-31 Applera Corporation High density sequence detection methods and apparatus
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005063787A2 (en) * 2003-12-23 2005-07-14 Applera Corporation Propargyl substituted nucleoside compounds and methods
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
EP1557464B1 (en) * 2004-01-23 2010-09-29 Sloning BioTechnology GmbH De novo enzymatic production of nucleic acid molecules
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
EP2208797A3 (en) 2004-03-01 2010-11-24 Applied Biosystems, LLC Methods, compositions and kits for use in polynucleotide amplification
CA2559171A1 (en) 2004-03-12 2005-09-29 Biotrove, Inc. Nanoliter array loading
GB2413796B (en) * 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
US9249456B2 (en) 2004-03-26 2016-02-02 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
US20060030037A1 (en) 2004-05-28 2006-02-09 Victor Joseph Thermo-controllable high-density chips for multiplex analyses
US8536661B1 (en) 2004-06-25 2013-09-17 University Of Hawaii Biosensor chip sensor protection methods
US20060073501A1 (en) * 2004-09-10 2006-04-06 Van Den Boom Dirk J Methods for long-range sequence analysis of nucleic acids
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7476503B2 (en) * 2004-09-17 2009-01-13 Pacific Biosciences Of California, Inc. Apparatus and method for performing nucleic acid analysis
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
KR100766750B1 (en) * 2004-12-13 2007-10-17 주식회사 엘지생명과학 Method for fabricating a biochip
US20060269934A1 (en) * 2005-03-16 2006-11-30 Applera Corporation Compositions and methods for clonal amplification and analysis of polynucleotides
US20060228721A1 (en) 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
CA2611671C (en) 2005-06-15 2013-10-08 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
WO2007053702A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3'-o-allyl modified photocleavable fluorescent nucleotides and related methods
US8796432B2 (en) 2005-10-31 2014-08-05 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-DNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods
WO2007120208A2 (en) * 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
US20080272006A1 (en) * 2005-11-30 2008-11-06 Zhiqiang Gao Electrochemical Method of Detecting an Analyte
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
WO2007092538A2 (en) * 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
WO2007111937A1 (en) 2006-03-23 2007-10-04 Applera Corporation Directed enrichment of genomic dna for high-throughput sequencing
RU2394915C2 (en) * 2006-03-24 2010-07-20 Александр Борисович Четверин Non-contact methods of detecting molecular colonies, sets of reagents and device for realising said methods
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
EP2078189B1 (en) * 2006-10-20 2012-10-10 Clondiag GmbH Assay devices and methods for the detection of analytes
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
WO2008069973A2 (en) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US7932034B2 (en) * 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
US8481259B2 (en) 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
US11035823B2 (en) 2009-03-17 2021-06-15 Qiagen Sciences, Llc Methods and devices for sequencing nucleic acids in smaller batches
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
US9029085B2 (en) * 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2008157640A2 (en) 2007-06-18 2008-12-24 Illumina, Inc. Microfabrication methods for the optimal patterning of substrates
WO2009011808A1 (en) * 2007-07-13 2009-01-22 President And Fellows Of Harvard College Droplet-based selection
WO2009054922A1 (en) 2007-10-19 2009-04-30 The Trustees Of Columbia University In The City Of New York Dna sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
EP4310194A2 (en) 2007-10-19 2024-01-24 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
EP2235210B1 (en) 2007-12-21 2015-03-25 President and Fellows of Harvard College Methods for nucleic acid sequencing
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
WO2009117698A2 (en) * 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
CA2719747C (en) 2008-03-28 2018-02-20 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US9115352B2 (en) * 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
CN102203282B (en) 2008-06-25 2014-04-30 生命技术公司 Methods and apparatus for measuring analytes using large scale FET arrays
US8198028B2 (en) * 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US20110218123A1 (en) * 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
EP3290531B1 (en) 2008-12-19 2019-07-24 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
WO2010117804A2 (en) * 2009-03-30 2010-10-14 The Regents Of The University Of California Mostly natural dna sequencing by synthesis
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
WO2011037990A1 (en) 2009-09-22 2011-03-31 President And Fellows Of Harvard College Entangled mate sequencing
EP3461558B1 (en) 2009-10-27 2021-03-17 President and Fellows of Harvard College Droplet creation techniques
CN106701739A (en) * 2009-12-04 2017-05-24 株式会社日立制作所 Analysis device and equipment for gene expression analysis
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
WO2012003380A2 (en) 2010-06-30 2012-01-05 Life Technologies Corporation Array column integrator
TWI539172B (en) 2010-06-30 2016-06-21 生命技術公司 Methods and apparatus for testing isfet arrays
CN103154718B (en) 2010-06-30 2015-09-23 生命科技公司 The electric charge accumulation circuit of sensing ion and method
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
TWI527245B (en) 2010-07-03 2016-03-21 生命技術公司 Chemically sensitive sensor with lightly doped drains
EP2617061B1 (en) 2010-09-15 2021-06-30 Life Technologies Corporation Methods and apparatus for measuring analytes
US8796036B2 (en) 2010-09-24 2014-08-05 Life Technologies Corporation Method and system for delta double sampling
US10392726B2 (en) 2010-10-08 2019-08-27 President And Fellows Of Harvard College High-throughput immune sequencing
CA2814049C (en) 2010-10-08 2021-07-13 President And Fellows Of Harvard College High-throughput single cell barcoding
US20150218639A1 (en) 2014-01-17 2015-08-06 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US10233501B2 (en) 2010-10-19 2019-03-19 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US20150225792A1 (en) 2014-01-17 2015-08-13 Northwestern University Compositions and methods for identifying depressive disorders
US10093981B2 (en) 2010-10-19 2018-10-09 Northwestern University Compositions and methods for identifying depressive disorders
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
WO2012162429A2 (en) 2011-05-23 2012-11-29 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using raman and infrared spectroscopy detection
WO2012170936A2 (en) 2011-06-09 2012-12-13 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
WO2013022778A1 (en) 2011-08-05 2013-02-14 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
CA3003082C (en) 2011-10-28 2020-12-15 Illumina, Inc. Microarray fabrication system and method
US8637242B2 (en) 2011-11-07 2014-01-28 Illumina, Inc. Integrated sequencing apparatuses and methods of use
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
WO2013096460A1 (en) 2011-12-20 2013-06-27 The Regents Of The University Of Michigan Pseudogenes and uses thereof
US9803188B2 (en) 2011-12-22 2017-10-31 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
WO2013096819A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
US9334491B2 (en) 2011-12-22 2016-05-10 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
WO2013102091A1 (en) 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
US9803231B2 (en) 2011-12-29 2017-10-31 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
CA2863121A1 (en) 2011-12-30 2013-07-04 Abbott Molecular Inc. Microorganism nucleic acid purification from host samples
CA2860594A1 (en) 2012-01-09 2013-07-18 Oslo Universitetssykehus Hf Methods and biomarkers for analysis of colorectal cancer
US20130184165A1 (en) 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
EP2817627A2 (en) 2012-02-21 2014-12-31 Oslo Universitetssykehus HF Methods and biomarkers for detection and prognosis of cervical cancer
US20150111758A1 (en) 2012-03-06 2015-04-23 Oslo Universitetssykehus Hf Gene signatures associated with efficacy of postmastectomy radiotherapy in breast cancer
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
WO2013152114A1 (en) 2012-04-03 2013-10-10 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and crohn's disease
ES2683707T3 (en) 2012-05-02 2018-09-27 Ibis Biosciences, Inc. DNA sequencing
ES2833524T3 (en) 2012-05-02 2021-06-15 Ibis Biosciences Inc DNA sequencing
US10544454B2 (en) 2012-05-02 2020-01-28 Ibis Biosciences, Inc. DNA sequencing
US20150133310A1 (en) 2012-05-02 2015-05-14 Ibis Biosciences, Inc. Nucleic acid sequencing systems and methods
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
MX364957B (en) 2012-08-14 2019-05-15 10X Genomics Inc Microcapsule compositions and methods.
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20150376609A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2895626B1 (en) 2012-09-12 2019-12-18 The Regents of The University of California Accurate genome sequencing of single cells by single-stranded amplification and sequencing
KR101595159B1 (en) * 2012-12-07 2016-02-18 서울대학교산학협력단 Isolation method of biochemical molecules on microarray
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
CA2900481A1 (en) 2013-02-08 2014-08-14 10X Genomics, Inc. Polynucleotide barcode generation
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
CA2902982C (en) 2013-03-08 2020-08-04 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
EP2964624B1 (en) 2013-03-08 2017-01-04 Illumina Cambridge Limited Rhodamine compounds and their use as fluorescent labels
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
CN105378108A (en) 2013-03-13 2016-03-02 雅培分子公司 Systems and methods for isolating nucleic acids
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
WO2014160117A1 (en) 2013-03-14 2014-10-02 Abbott Molecular Inc. Multiplex methylation-specific amplification systems and methods
WO2014152937A1 (en) 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
EP2972279B1 (en) 2013-03-15 2021-10-06 Life Technologies Corporation Chemical sensors with consistent sensor surface areas
CN105051525B (en) 2013-03-15 2019-07-26 生命科技公司 Chemical device with thin conducting element
CN108864232B (en) 2013-03-15 2022-01-04 伊鲁米纳剑桥有限公司 Modified nucleosides or nucleotides
US9890425B2 (en) 2013-03-15 2018-02-13 Abbott Molecular Inc. Systems and methods for detection of genomic copy number changes
CN105264366B (en) 2013-03-15 2019-04-16 生命科技公司 Chemical sensor with consistent sensor surface area
US20160153029A1 (en) 2013-03-15 2016-06-02 Ibis Biosciences, Inc. Dna sequences to assess contamination in dna sequencing
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
EP3919624A3 (en) 2013-07-01 2021-12-29 Illumina, Inc. Catalyst-free surface functionalization and polymer grafting
JP6300260B2 (en) * 2013-08-28 2018-03-28 国立大学法人埼玉大学 Method for producing replica microarray and original microarray containing target substance produced by the method
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
DK3572527T3 (en) 2013-10-17 2021-08-23 Illumina Inc METHODS AND PREPARATIONS FOR THE MANUFACTURE OF NUCLEIC ACID LIBRARIES
EP3065712A4 (en) 2013-11-08 2017-06-21 President and Fellows of Harvard College Microparticles, methods for their preparation and use
CN111118121A (en) 2013-12-05 2020-05-08 生捷科技控股公司 Preparation of patterned arrays
US10597715B2 (en) 2013-12-05 2020-03-24 Centrillion Technology Holdings Methods for sequencing nucleic acids
EP3077430A4 (en) 2013-12-05 2017-08-16 Centrillion Technology Holdings Corporation Modified surfaces
AU2014364180B2 (en) 2013-12-09 2021-03-04 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
US9909181B2 (en) 2013-12-13 2018-03-06 Northwestern University Biomarkers for post-traumatic stress states
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
EP3083700B1 (en) 2013-12-17 2023-10-11 The Brigham and Women's Hospital, Inc. Detection of an antibody against a pathogen
CN114089597A (en) 2013-12-19 2022-02-25 Illumina公司 Substrate comprising a nanopatterned surface and method for the production thereof
WO2015103225A1 (en) 2013-12-31 2015-07-09 Illumina, Inc. Addressable flow cell using patterned electrodes
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
US20150203907A1 (en) * 2014-01-17 2015-07-23 Florida State University Research Foundation Genome capture and sequencing to determine genome-wide copy number variation
US11060139B2 (en) 2014-03-28 2021-07-13 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
EP3151733B1 (en) 2014-06-06 2020-04-15 The Regents Of The University Of Michigan Compositions and methods for characterizing and diagnosing periodontal disease
CN114214314A (en) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 Digital PCR barcoding
JP2017526046A (en) 2014-06-26 2017-09-07 １０エックス ゲノミクス，インコーポレイテッド Nucleic acid sequence assembly process and system
ES2753573T3 (en) 2014-06-27 2020-04-13 Abbott Lab Compositions and methods for detecting human pegivirus 2 (HPGV-2)
EP3191607B1 (en) 2014-07-24 2019-08-21 Abbott Molecular Inc. Compositions for the detection and analysis of mycobacterium tuberculosis
US10550428B2 (en) 2014-09-17 2020-02-04 Ibis Biosciences, Inc. Sequencing by synthesis using pulse read optics
US20160122817A1 (en) 2014-10-29 2016-05-05 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US11198855B2 (en) 2014-11-13 2021-12-14 The Board Of Trustees Of The University Of Illinois Bio-engineered hyper-functional “super” helicases
US10605767B2 (en) 2014-12-18 2020-03-31 Life Technologies Corporation High data rate integrated circuit with transmitter configuration
CN111505087A (en) 2014-12-18 2020-08-07 生命科技公司 Method and apparatus for measuring analytes using large scale FET arrays
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
KR20170106979A (en) 2015-01-13 2017-09-22 10엑스 제노믹스, 인크. System and method for visualizing structure variation and phase adjustment information
US10854315B2 (en) 2015-02-09 2020-12-01 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
DK3259602T3 (en) 2015-02-20 2021-02-15 Takara Bio Usa Inc PROCEDURE FOR FAST ACCURATE DISPENSATION, VISUALIZATION AND ANALYSIS OF SINGLE CELLS
EP3262188B1 (en) 2015-02-24 2021-05-05 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
GB201508858D0 (en) 2015-05-22 2015-07-01 Illumina Cambridge Ltd Polymethine compounds with long stokes shifts and their use as fluorescent labels
EP3527672B1 (en) * 2015-06-09 2022-10-05 Centrillion Technology Holdings Corporation Oligonucleotide arrays for sequencing nucleic acids
EP3322483A4 (en) 2015-07-14 2019-01-02 Abbott Molecular Inc. Compositions and methods for identifying drug resistant tuberculosis
EP3988658A1 (en) 2015-07-14 2022-04-27 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides
JP2018527947A (en) 2015-07-17 2018-09-27 プレジデント アンド フェローズ オブ ハーバード カレッジ Nucleic acid sequence amplification method
ES2945607T3 (en) 2015-07-17 2023-07-04 Illumina Inc Polymer sheets for sequencing applications
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
CN108779138B (en) 2015-09-28 2022-06-17 哥伦比亚大学董事会 Design and synthesis of nucleotides based on novel disulfide linkers for use as reversible terminators for DNA sequencing by synthesis
CN108289797B (en) 2015-10-13 2022-01-28 哈佛学院院长及董事 Systems and methods for making and using gel microspheres
WO2017066561A2 (en) 2015-10-16 2017-04-20 President And Fellows Of Harvard College Regulatory t cell pd-1 modulation for regulating t cell effector immune responses
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
CN108431223A (en) 2016-01-08 2018-08-21 生物辐射实验室股份有限公司 Multiple pearls under per drop resolution
EP3414324B1 (en) 2016-02-08 2021-01-20 Rgene, Inc. Multiple ligase compositions, systems, and methods
SG11201806757XA (en) 2016-02-11 2018-09-27 10X Genomics Inc Systems, methods, and media for de novo assembly of whole genome sequence data
CN109477127A (en) 2016-03-14 2019-03-15 R基因股份有限公司 Uht-stable lysine-saltant type ssDNA/RNA ligase
US20210309965A1 (en) 2016-03-21 2021-10-07 Dana-Farber Cancer Institute, Inc. T-cell exhaustion state-specific gene expression regulators and uses thereof
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10900076B2 (en) 2016-05-18 2021-01-26 Illumina, Inc. Self assembled patterning using patterned hydrophobic surfaces
US11118233B2 (en) 2016-05-18 2021-09-14 The University Of Chicago BTK mutation and ibrutinib resistance
US11091795B2 (en) 2016-07-11 2021-08-17 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
WO2018017892A1 (en) 2016-07-21 2018-01-25 Takara Bio Usa, Inc. Multi-z imaging and dispensing with multi-well devices
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
US11530352B2 (en) 2016-10-03 2022-12-20 Illumina, Inc. Fluorescent detection of amines and hydrazines and assaying methods thereof
CN110139932A (en) 2016-12-19 2019-08-16 生物辐射实验室股份有限公司 The labeling DNA that the adjacency of drop mark-on retains
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
ES2954284T3 (en) 2016-12-22 2023-11-21 Illumina Cambridge Ltd Coumarin compounds and their uses as fluorescent labels
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
EP3596099A4 (en) 2017-03-06 2020-12-09 Singular Genomics Systems, Inc. Nucleic acid sequencing-by-synthesis (sbs) methods that combine sbs cycle steps
EP3601593B1 (en) 2017-03-24 2021-12-22 Bio-Rad Laboratories, Inc. Universal hairpin primers
CN106987645A (en) * 2017-05-05 2017-07-28 武汉爱基百客生物科技有限公司 A kind of apparent omics technology
EP3625715A4 (en) 2017-05-19 2021-03-17 10X Genomics, Inc. Systems and methods for analyzing datasets
CN116064732A (en) 2017-05-26 2023-05-05 10X基因组学有限公司 Single cell analysis of transposase accessibility chromatin
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3634992B1 (en) 2017-06-08 2024-03-27 The Brigham And Women's Hospital, Inc. Methods and compositions for identifying epitopes
US11186862B2 (en) 2017-06-20 2021-11-30 Bio-Rad Laboratories, Inc. MDA using bead oligonucleotide
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
WO2019035955A1 (en) * 2017-08-15 2019-02-21 President And Fellows Of Harvard College High-throughput system using a cell-free expression system and in situ sequencing
CN111787930A (en) 2017-10-06 2020-10-16 芝加哥大学 Screening of T lymphocytes against cancer specific antigens
GB201716931D0 (en) 2017-10-16 2017-11-29 Illumina Cambridge Ltd New fluorescent compounds and their use as biomarkers
EP4180534A1 (en) 2017-11-02 2023-05-17 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3714043A4 (en) 2017-11-22 2021-08-11 The Regents of The University of Michigan Compositions and methods for treating cancer
KR20200105486A (en) * 2017-12-28 2020-09-07 아도르 디아그노스틱스 에스.알.엘. Method for rapidly detecting the presence of nucleic acid target molecules
US11701656B2 (en) 2018-01-02 2023-07-18 The Regents Of The University Of Michigan Multi-droplet capture
CN112041459A (en) 2018-01-29 2020-12-04 圣祖德儿童研究医院 Nucleic acid amplification method
WO2019152395A1 (en) 2018-01-31 2019-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
WO2019191122A1 (en) 2018-03-26 2019-10-03 Qiagen Sciences, Llc Integrative dna and rna library preparations and uses thereof
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
WO2019213619A1 (en) 2018-05-04 2019-11-07 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
EP3836967A4 (en) 2018-07-30 2022-06-15 ReadCoor, LLC Methods and systems for sample processing or analysis
US11479816B2 (en) 2018-08-20 2022-10-25 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
EP3870593A4 (en) 2018-10-25 2022-11-16 Singular Genomics Systems, Inc. Nucleotide analogues
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US20220081714A1 (en) 2019-01-04 2022-03-17 Northwestern University Storing temporal data into dna
EP3908286A4 (en) 2019-01-08 2022-10-12 Singular Genomics Systems, Inc. Nucleotide cleavable linkers and uses thereof
CN113710686A (en) 2019-02-19 2021-11-26 阿尔缇玛基因组学公司 Linker and method for optical detection and sequencing
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
EP3931190A1 (en) 2019-03-01 2022-01-05 Illumina Cambridge Limited Tertiary amine substituted coumarin compounds and their uses as fluorescent labels
AU2020230955A1 (en) 2019-03-01 2021-01-07 Illumina Cambridge Limited Exocyclic amine-substituted coumarin compounds and their uses as fluorescent labels
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
JP2023510454A (en) 2019-11-27 2023-03-14 イルミナ ケンブリッジ リミテッド Pigments and compositions containing cyclooctatetraene
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
EP3859012A1 (en) 2020-02-03 2021-08-04 Albert-Ludwigs-Universität Freiburg Methods for amplification of genomic dna and preparation of sequencing libraries
CN115349128A (en) 2020-02-13 2022-11-15 齐默尔根公司 Metagenomic libraries and natural product discovery platform
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
CN116134152A (en) 2020-05-27 2023-05-16 基因技术有限公司 Method for assessing risk of producing a severe response to coronavirus infection
PE20230492A1 (en) 2020-06-22 2023-03-23 Illumina Cambridge Ltd NUCLEOSIDES AND NUCLEOTIDES WITH 3' ACETAL BLOCKING GROUP
US20220033900A1 (en) 2020-07-28 2022-02-03 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
US20240043915A1 (en) 2021-02-13 2024-02-08 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
EP4334327A1 (en) 2021-05-05 2024-03-13 Illumina Cambridge Limited Fluorescent dyes containing bis-boron fused heterocycles and uses in sequencing
CN117916390A (en) 2021-05-20 2024-04-19 伊鲁米纳公司 Compositions and methods for sequencing-by-synthesis
CA3222829A1 (en) 2021-12-16 2023-06-22 Gabriele CANZI Methods for metal directed cleavage of surface-bound polynucleotides
AU2022421156A1 (en) 2021-12-20 2024-01-18 Illumina, Inc. Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
AU2022419500A1 (en) 2021-12-20 2024-01-18 Illumina, Inc. Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
FR3130844A1 (en) 2021-12-22 2023-06-23 Bforcure COMPOSITION AND METHOD FOR AMPLIFICATION OF NUCLEIC ACID SEQUENCES
WO2023135485A1 (en) 2022-01-13 2023-07-20 Oslo Universitetssykehus Hf Prostate cancer markers and uses thereof
WO2023152568A2 (en) 2022-02-10 2023-08-17 Oslo Universitetssykehus Hf Compositions and methods for characterizing lung cancer
US20230304086A1 (en) 2022-03-28 2023-09-28 Illumina Cambridge Limited Labeled avidin and methods for sequencing
WO2023186819A1 (en) 2022-03-29 2023-10-05 Illumina Cambridge Limited Chromenoquinoline dyes and uses in sequencing
AU2023246691A1 (en) 2022-03-30 2024-01-18 Illumina, Inc. Methods for chemical cleavage of surface-bound polynucleotides
AU2023246851A1 (en) 2022-03-31 2024-01-18 Illumina, Inc. Compositions and methods for improving sequencing signals
US20230332197A1 (en) 2022-03-31 2023-10-19 Illumina Singapore Pte. Ltd. Nucleosides and nucleotides with 3' vinyl blocking group
US20230383342A1 (en) 2022-05-31 2023-11-30 Illumina Cambridge Limited Compositions and methods for nucleic acid sequencing
WO2024003087A1 (en) 2022-06-28 2024-01-04 Illumina, Inc. Fluorescent dyes containing fused tetracyclic bis-boron heterocycle and uses in sequencing
WO2024015766A1 (en) 2022-07-12 2024-01-18 Topogene Inc. Scalable, submicron-resolution replication of dna arrays
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection
WO2024068889A2 (en) 2022-09-30 2024-04-04 Illumina, Inc. Compositions and methods for reducing photo damage during sequencing

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591567A (en) 1982-04-21 1986-05-27 California Institute Of Technology Recombinant DNA screening system including fixed array replicator and support
FI71768C (en) 1984-02-17 1987-02-09 Orion Yhtymae Oy Enhanced nucleic acid reagents and process for their preparation.
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US4981783A (en) 1986-04-16 1991-01-01 Montefiore Medical Center Method for detecting pathological conditions
SE8801070D0 (en) 1988-03-23 1988-03-23 Pharmacia Ab METHOD FOR IMMOBILIZING A DNA SEQUENCE ON A SOLID SUPPORT
US5188963A (en) 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US5382511A (en) 1988-08-02 1995-01-17 Gene Tec Corporation Method for studying nucleic acids within immobilized specimens
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5449603A (en) * 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
CA2031659A1 (en) 1990-01-26 1991-07-27 John B. Findlay Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods
US5506350A (en) 1990-09-20 1996-04-09 Bittner; Michael L. Production of chromosome region specific DNA sequences and transamination
RU1794088C (en) 1991-03-18 1993-02-07 Институт Молекулярной Биологии Ан@ Ссср Method of dna nucleotide sequence determination and a device for its realization
US5426180A (en) 1991-03-27 1995-06-20 Research Corporation Technologies, Inc. Methods of making single-stranded circular oligonucleotides
DK0593618T3 (en) 1991-06-27 1998-11-23 Genelabs Tech Inc Screening assay for detection of DNA-binding molecules
US5578444A (en) 1991-06-27 1996-11-26 Genelabs Technologies, Inc. Sequence-directed DNA-binding molecules compositions and methods
US5437976A (en) 1991-08-08 1995-08-01 Arizona Board Of Regents, The University Of Arizona Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA
EP0672173B1 (en) * 1991-11-01 2002-08-28 Diatech Pty. Ltd. Solid phase amplification process
US5846708A (en) 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
US5324633A (en) 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
DE69334048D1 (en) * 1992-01-28 2006-08-31 Harvard College PROCESS FOR NUCLEIN ACID HIBRIDIZATION USING UNIVERSAL DNA BINDING PROTEINS
DE69333650T2 (en) 1992-02-19 2006-01-12 The Public Health Research Institute Of The City Of New York, Inc. NEW ARRANGEMENT OF OLIGONUCLEOTIDES AND THEIR BENEFITS FOR SORTING, ISOLATING, SEQUENCING AND MANIPULATING NUCLEIC ACIDS
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
RU2048522C1 (en) 1992-10-14 1995-11-20 Институт белка РАН Method of nucleic acid copying, method of their expression and a medium for their realization
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5503980A (en) 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5484702A (en) 1994-01-27 1996-01-16 Research Foundation Of The State University Of New York At Buffalo Method for preselecting recombinant clones containing a specific nucleic acid sequence and subsequent transformation with preselected clones
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US5571639A (en) 1994-05-24 1996-11-05 Affymax Technologies N.V. Computer-aided engineering system for design of sequence arrays and lithographic masks
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
FR2726286B1 (en) * 1994-10-28 1997-01-17 Genset Sa SOLID PHASE NUCLEIC ACID AMPLIFICATION PROCESS AND REAGENT KIT USEFUL FOR CARRYING OUT SAID PROCESS
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5908926A (en) 1995-03-16 1999-06-01 Duke University 5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group
US5753439A (en) 1995-05-19 1998-05-19 Trustees Of Boston University Nucleic acid detection methods
US5545531A (en) 1995-06-07 1996-08-13 Affymax Technologies N.V. Methods for making a device for concurrently processing multiple biological chip assays
GB9517914D0 (en) 1995-09-02 1995-11-01 Tepnel Medical Ltd Identification of bases in nucleic acid sequences
JP4663824B2 (en) 1996-12-31 2011-04-06 ハイ スループット ジェノミクス インコーポレイテッド Multiplexed molecular analyzer and method
ES2563643T3 (en) 1997-04-01 2016-03-15 Illumina Cambridge Limited Nucleic acid sequencing method
DE69824716D1 (en) 1997-04-01 2004-07-29 Manteia S A METHOD FOR SEQUENCING NUCLEIC ACIDS
JP4313861B2 (en) 1997-08-01 2009-08-12 キヤノン株式会社 Manufacturing method of probe array
CA2325399A1 (en) * 1998-03-23 1999-09-30 Invitrogen Corporation Modified nucleotides and methods useful for nucleic acid sequencing

Also Published As

Publication number Publication date
EP1291354A2 (en) 2003-03-12
EP1235929A2 (en) 2002-09-04
AU3876100A (en) 2000-09-28
CA2411514A1 (en) 2000-09-14
WO2000053812A9 (en) 2001-10-25
JP2003526331A (en) 2003-09-09
US20020120126A1 (en) 2002-08-29
WO2000053812A2 (en) 2000-09-14
EP1291354A3 (en) 2003-12-10
CA2370535C (en) 2008-05-13
US20020120127A1 (en) 2002-08-29
WO2000053812A3 (en) 2002-07-11
US6485944B1 (en) 2002-11-26
CA2582809A1 (en) 2000-09-14

Similar Documents

Publication Publication Date Title
CA2370535A1 (en) Replica amplification of nucleic acid arrays
WO2004067759A3 (en) Replication of nucleic acid arrays
EP1343874A4 (en) 3-hydroxypropionic acid and other organic compounds
DE69933369D1 (en) Adressierbare protein arrays
WO2002048755A3 (en) Resolution modulation in microlens image reproduction
CA2205353A1 (en) Continuous amplification reaction
CA2020958A1 (en) Nucleic acid sequence amplification methods
AU2001245945A1 (en) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
PT1309861E (en) FUNCTIONAL ARRAYS OF PROTEINS
WO2005074467A3 (en) Matrix array nanobiosensor
WO2001059432A3 (en) Array of individual arrays as substrate for bead-based simultaneous processing of samples and manufacturing method therefor
CY1107918T1 (en) DIAGNOSIS LAMINS
MXPA03001879A (en) Method and system for detecting nucleic acids.
JP2001521765A5 (en)
WO2001031055A3 (en) Method and kit for the screening and/or the quantification of multiple homologous nucleic acid sequences on arrays
DE69916228D1 (en) POWDER FILM-FORMING POLYMERS REDISPERGABLE IN WATER, MADE FROM ETHYLENIC-UNSATURATED MONOMERS, CONTAINING NAPHTHALINE SULPHONATES
WO2001035072A3 (en) A system for cell-based screening
WO1992020796A3 (en) D-type cyclin and uses related thereto
EP0938097A3 (en) Memory reading circuit and SRAM
CA2414618A1 (en) Composite arrays utilizing microspheres with a hybridization chamber
RU95115686A (en) PREMERSACIDINE, PROMERSACIDINE, DNA, VECTOR, HOST CELL, METHOD FOR PRODUCING
EP1264894A4 (en) Atp regeneration reaction systems and method of examining adenine nucleotide, method of detecting rna and method of amplifying atp by using the
CA2185362A1 (en) Purified nucleic acid encoding a thermostable pyrophosphatase
FR2563533B1 (en) METHOD FOR AMPLIFYING THE EXPRESSION OF A DETERMINED GENE IN SUBACTIVE BACILLUS AND STRAINS OBTAINED
DK1003525T3 (en) Preparation and method for controlling cell proliferation and cell death

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20200310