CA2363537A1 - Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods - Google Patents

Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods Download PDF

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Publication number
CA2363537A1
CA2363537A1 CA002363537A CA2363537A CA2363537A1 CA 2363537 A1 CA2363537 A1 CA 2363537A1 CA 002363537 A CA002363537 A CA 002363537A CA 2363537 A CA2363537 A CA 2363537A CA 2363537 A1 CA2363537 A1 CA 2363537A1
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CA
Canada
Prior art keywords
seq
cgb
pde
polypeptide
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002363537A
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French (fr)
Other versions
CA2363537C (en
Inventor
Joseph A. Beavo
Jackie D. Corbin
Kenneth M. Ferguson
Sharron H. Francis
Ann Kadlecek
Kate Loughney
Linda M. Mcallister-Lucas
William K. Sonnenburg
Melissa K. Thomas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Icos Corp
Original Assignee
Board Of Regents Of The University Of Washington
Joseph A. Beavo
Jackie D. Corbin
Kenneth M. Ferguson
Sharron H. Francis
Ann Kadlecek
Kate Loughney
Linda M. Mcallister-Lucas
William K. Sonnenburg
Melissa K. Thomas
Vanderbilt University
The Board Of Regents Of The University Of Washington
Icos Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22080121&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2363537(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Board Of Regents Of The University Of Washington, Joseph A. Beavo, Jackie D. Corbin, Kenneth M. Ferguson, Sharron H. Francis, Ann Kadlecek, Kate Loughney, Linda M. Mcallister-Lucas, William K. Sonnenburg, Melissa K. Thomas, Vanderbilt University, The Board Of Regents Of The University Of Washington, Icos Corporation filed Critical Board Of Regents Of The University Of Washington
Priority to CA2698716A priority Critical patent/CA2698716A1/en
Publication of CA2363537A1 publication Critical patent/CA2363537A1/en
Application granted granted Critical
Publication of CA2363537C publication Critical patent/CA2363537C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Abstract

The present invention provides novel purified and isolated nucleotide sequences encoding the cGMP-binding, eGMP-specific phosphodiesterase designated cGB-PDE. Also provided by the invention are methods and materials for the recombinant production of cGB-PDE polypeptide products and methods for identifying compounds which modulate the enzymatic activity of cGB-PDE polypeptides.

Claims (28)

1. A purified and isolated polynucleotide encoding the cyclic GMP binding phosphodiesterase (cGB-PDE) polypeptide set out in SEQ ID NO:10 or SEQ ID NO:23.
2. The polynucleotide of claim 1 which is a DNA.
3. The DNA of claim 2 which is a cDNA.
4. The DNA of claim 2 which is a genomic DNA.
5. An RNA transcript of the DNA of claim 2.
6. The DNA of claim 4 further comprising an endogenous expression control DNA sequence.
7. A vector comprising a DNA according to claim 2.
8. The vector of claim 7, wherein said DNA is operatively linked to an expression control sequence.
9. A host cell stably transformed or transfected with a DNA according to claim 2 in a manner allowing the expression in said host cell of the cGB-PDE of SEQ ID NO:10 or-23.
10. A method of producing a cGB-PDE polypeptide, said method comprising growing a host cell according to claim 9 in a suitable nutrient medium and isolating cGB-PDE polypeptide from said cell or the medium of its growth.
11. A polypeptide or peptide capable of specifically binding to the cyclic GMP binding phosphodiesterase of SEQ ID NO:10 or SEQ ID NO:23.
12. A purified and isolated polynucleotide selected from the group consisting of a polynucleotide encoding an allelic variant of cGB-PDE polypeptide of SEQ ID NO:23 and a polynucleotide encoding a non-human species cGB-PDE homolog, wherein said polynucleotide hybridizes at about: 65ÀC in 3X
SSC, 20 mM sodium phosphate pH 6.8, with washing at about:
65ÀC in 2X SSC, to a non-coding strand of DNA of SEQ ID NO:22.
13. A purified and isolated polypeptide encoded by a DNA sequence of SEQ ID NO: 9 or SEQ ID NO:22.
14. A purified and isolated recombinant human cyclic GMP binding phosphodiesterase (cGB-PDE) polypeptide comprising an amino acid sequence according to SEQ ID NO:23.
15. A fragment of the cGB-PDE polypeptide of claim 14 comprising amino acids 516 to 875 of SEQ ID NO:23.
16. A fragment of the cGB-PDE polypeptide of claim 14 comprising the amino acids 1-494 of SEQ ID NO:23.
17. A fragment of the cGB-PDE polypeptide of claim 14 comprising the amino acids 1-549 of SEQ ID NO:23.
18. A purified and isolated recombinant bovine cyclic GMP binding phosphodiessterase (cGB-PDE) polypeptide comprising an amino acid sequence according to SEQ ID NO:10.
19. An analog of the cGB-PDE polypeptide of claim 18, wherein the amino acid at position 289 of SEQ ID NO:10 is an alanine.
20. An analog of the cGB-PDE polypeptide of claim 18, wherein the amino acid at position 478 of SEQ ID NO:10 is an alanine.
21. An antibody specifically immunoreactive with the bovine cGB-PDE polypeptide of SEQ ID NO:10.
22. A monoclonal antibody according to claim 21.
23. A hybridoma cell line producing the monoclonal antibody according to claim 22.
24. A humanized antibody according to claim 21.
25. An antibody specifically immunoreactive with the human cGB-PDE polypeptide of SEQ ID NO:23.
26. A monoclonal antibody according to claim 25.
27. A hybridoma cell line producing the antibody of claim 26.
28. A humanized antibody according to claim 25.
CA2363537A 1993-05-27 1994-05-27 Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods Expired - Fee Related CA2363537C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2698716A CA2698716A1 (en) 1993-05-27 1994-05-27 Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6805193A 1993-05-27 1993-05-27
US08/068,051 1993-05-27
CA002141060A CA2141060C (en) 1993-05-27 1994-05-27 Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA002141060A Division CA2141060C (en) 1993-05-27 1994-05-27 Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2698716A Division CA2698716A1 (en) 1993-05-27 1994-05-27 Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods

Publications (2)

Publication Number Publication Date
CA2363537A1 true CA2363537A1 (en) 1994-12-08
CA2363537C CA2363537C (en) 2010-07-06

Family

ID=22080121

Family Applications (3)

Application Number Title Priority Date Filing Date
CA2698716A Abandoned CA2698716A1 (en) 1993-05-27 1994-05-27 Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods
CA002141060A Expired - Fee Related CA2141060C (en) 1993-05-27 1994-05-27 Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods
CA2363537A Expired - Fee Related CA2363537C (en) 1993-05-27 1994-05-27 Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods

Family Applications Before (2)

Application Number Title Priority Date Filing Date
CA2698716A Abandoned CA2698716A1 (en) 1993-05-27 1994-05-27 Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods
CA002141060A Expired - Fee Related CA2141060C (en) 1993-05-27 1994-05-27 Cyclic gmp-binding, cyclic gmp-specific phosphodiesterase materials and methods

Country Status (13)

Country Link
US (7) US5702936A (en)
EP (2) EP1038963B1 (en)
JP (3) JP4150070B2 (en)
AT (2) ATE199569T1 (en)
CA (3) CA2698716A1 (en)
DE (3) DE69426804T4 (en)
DK (2) DK1038963T3 (en)
ES (2) ES2333691T3 (en)
GR (1) GR3035616T3 (en)
HK (1) HK1013427A1 (en)
LV (1) LV12814B (en)
PT (2) PT652960E (en)
WO (1) WO1994028144A1 (en)

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WO1999042596A2 (en) * 1998-02-23 1999-08-26 Icos Corporation Phosphodiesterase 10
WO2000005383A1 (en) * 1998-07-21 2000-02-03 Takeda Chemical Industries, Ltd. Novel protein and its dna
EP1133689A4 (en) * 1998-11-25 2002-10-30 Cell Pathways Inc Method for diagnosing neoplasia
US6875575B1 (en) 1998-11-25 2005-04-05 Osi Pharmaceuticals, Inc. Diagnostic methods for neoplasia
US6100037A (en) * 1999-01-07 2000-08-08 Incyte Pharmaceuticals, Inc. Human cyclic nucleotide PDEs
US6451807B1 (en) 1999-04-30 2002-09-17 Lilly Icos, Llc. Methods of treating sexual dysfunction in an individual suffering from a retinal disease, class 1 congestive heart failure, or myocardial infarction using a PDE5 inhibitor
US6146876A (en) * 1999-06-11 2000-11-14 Millennium Pharmaceuticals, Inc. 22025, a novel human cyclic nucleotide phosphodiesterase
US7098015B2 (en) * 1999-06-11 2006-08-29 Millennium Pharmaceuticals, Inc. 27875, 22025, 27420, 17906, 16319, 55092 and 10218 molecules and uses therefor
GB9922125D0 (en) 1999-09-17 1999-11-17 Pfizer Ltd Phosphodiesterase enzymes
CN1297049A (en) * 1999-11-23 2001-05-30 上海博容基因开发有限公司 Polypeptide-human cyclophosphate guanine suppressed phosphodiesterase 17 and polynucleotide for coding said polypeptide
JP2004500425A (en) 2000-04-19 2004-01-08 リリー　アイコス　リミテッド　ライアビリティ　カンパニー Use of cyclic GMP-specific phosphodiesterase inhibitors for the treatment of Parkinson's disease
GB0016009D0 (en) * 2000-06-29 2000-08-23 Pfizer Ltd Target
US6794192B2 (en) 2000-06-29 2004-09-21 Pfizer Inc. Target
US6846813B2 (en) * 2000-06-30 2005-01-25 Pharmacia & Upjohn Company Compounds to treat alzheimer's disease
US20030096864A1 (en) * 2000-06-30 2003-05-22 Fang Lawrence Y. Compounds to treat alzheimer's disease
ES2291362T3 (en) * 2000-09-14 2008-03-01 Mount Sinai School Of Medicine SCREENING PROCEDURES TO IDENTIFY G PROTEINS AND OTHER COMPOUNDS THAT MODULATE PHOSPHODESTERASE ACTIVITY (PDE).
US7803982B2 (en) 2001-04-20 2010-09-28 The Mount Sinai School Of Medicine Of New York University T1R3 transgenic animals, cells and related methods
US20040219632A1 (en) * 2001-04-20 2004-11-04 Robert Margolskee T1r3 a novel taste receptor
US20070213407A1 (en) * 2001-06-29 2007-09-13 Elan Pharmaceuticals And Pharmacia & Upjohn Company Llc Compounds to treat Alzheimer's disease
EP2351765A3 (en) 2001-07-10 2012-02-22 Lakewood-Amedex, Inc Oligonucleotide-containing pharmacological compositions and their use
US20050202549A1 (en) * 2001-11-02 2005-09-15 Pfizer Inc Crystal structure
GB0126417D0 (en) * 2001-11-02 2002-01-02 Pfizer Ltd Crystal structure
US7304128B2 (en) * 2002-06-04 2007-12-04 E.I. Du Pont De Nemours And Company Carbon nanotube binding peptides
CA2522472A1 (en) * 2003-05-01 2004-11-11 Pfizer Inc. Crystal of pde5, its crystal structure and its use in drug design
JP2010004758A (en) * 2008-06-24 2010-01-14 Probex Inc Cyclic gmp detection method
US20110262989A1 (en) 2010-04-21 2011-10-27 Nanomr, Inc. Isolating a target analyte from a body fluid
US8841104B2 (en) 2010-04-21 2014-09-23 Nanomr, Inc. Methods for isolating a target analyte from a heterogeneous sample
US9476812B2 (en) 2010-04-21 2016-10-25 Dna Electronics, Inc. Methods for isolating a target analyte from a heterogeneous sample
US10000557B2 (en) 2012-12-19 2018-06-19 Dnae Group Holdings Limited Methods for raising antibodies
US9995742B2 (en) 2012-12-19 2018-06-12 Dnae Group Holdings Limited Sample entry
US9434940B2 (en) 2012-12-19 2016-09-06 Dna Electronics, Inc. Methods for universal target capture
US9804069B2 (en) 2012-12-19 2017-10-31 Dnae Group Holdings Limited Methods for degrading nucleic acid
US9599610B2 (en) 2012-12-19 2017-03-21 Dnae Group Holdings Limited Target capture system
US9551704B2 (en) 2012-12-19 2017-01-24 Dna Electronics, Inc. Target detection
KR101550217B1 (en) * 2014-03-26 2015-09-04 전남대학교산학협력단 Recombinant vector for foreign gene expression without biological circuit interference of host cell and uses thereof
WO2017094885A1 (en) * 2015-12-04 2017-06-08 国立大学法人東京大学 Ligand fluorescent sensor protein and use thereof

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Also Published As

Publication number Publication date
JP2010042035A (en) 2010-02-25
DE69426804T4 (en) 2005-10-13
WO1994028144A1 (en) 1994-12-08
LV12814B (en) 2002-10-20
CA2141060C (en) 2002-02-19
LV12814A (en) 2002-04-20
EP1038963B1 (en) 2009-08-05
US20060216809A1 (en) 2006-09-28
US5955583A (en) 1999-09-21
JP4450767B2 (en) 2010-04-14
EP0652960B1 (en) 2001-03-07
ATE199569T1 (en) 2001-03-15
ES2156898T3 (en) 2001-08-01
US5702936A (en) 1997-12-30
US20050196833A1 (en) 2005-09-08
DK1038963T3 (en) 2009-11-23
ES2333691T3 (en) 2010-02-26
US20030054992A1 (en) 2003-03-20
PT1038963E (en) 2009-10-13
DE69435227D1 (en) 2009-09-17
US6037119A (en) 2000-03-14
CA2698716A1 (en) 1994-12-08
US5652131A (en) 1997-07-29
DK0652960T3 (en) 2001-04-17
DE69426804T2 (en) 2001-07-19
GR3035616T3 (en) 2001-06-29
JP4150070B2 (en) 2008-09-17
ATE438723T1 (en) 2009-08-15
DE69426804D1 (en) 2001-04-12
JP2005245467A (en) 2005-09-15
EP1038963A2 (en) 2000-09-27
CA2141060A1 (en) 1994-12-08
JPH08502900A (en) 1996-04-02
EP1038963A3 (en) 2000-10-04
PT652960E (en) 2001-08-30
EP0652960A1 (en) 1995-05-17
HK1013427A1 (en) 1999-08-27
CA2363537C (en) 2010-07-06
EP0652960B9 (en) 2004-10-27

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