CA2352362A1 - Nanoparticles with polymer shells - Google Patents

Nanoparticles with polymer shells Download PDF

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Publication number
CA2352362A1
CA2352362A1 CA002352362A CA2352362A CA2352362A1 CA 2352362 A1 CA2352362 A1 CA 2352362A1 CA 002352362 A CA002352362 A CA 002352362A CA 2352362 A CA2352362 A CA 2352362A CA 2352362 A1 CA2352362 A1 CA 2352362A1
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CA
Canada
Prior art keywords
monomers
nanoparticles
formula
provides
initiation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002352362A
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French (fr)
Other versions
CA2352362C (en
Inventor
Chad A. Mirkin
Sonbinh T. Nguyen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanosphere LLC
Original Assignee
Nanosphere, Inc.
Chad A. Mirkin
Sonbinh T. Nguyen
Nanosphere Llc
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Application filed by Nanosphere, Inc., Chad A. Mirkin, Sonbinh T. Nguyen, Nanosphere Llc filed Critical Nanosphere, Inc.
Publication of CA2352362A1 publication Critical patent/CA2352362A1/en
Application granted granted Critical
Publication of CA2352362C publication Critical patent/CA2352362C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label

Abstract

The invention provides a method of preparing nanoparticles having at least one polymer shell attached to them, each polymer shell having a selected property or properties. The method comprises attaching initiation monomers to the surfaces of the nanoparticles, contacting the nanoparticles having the initiation monomers attached to them with a transition metal ring-opening metathesis catalyst to activate the initiation monomers, and contacting the nanoparticles with one or more types of propagation monomers of the formula P-L-N under conditions effective so that the monomers are polymerized to form the one or more polymer shells. In the formula P-L-N, N is a cyclic olefin-containing group, P is a moiety which gives each polymer shell a selected property or properties, and L is a bond or linker. The invention also provides polymers formed by polymerizing the propagation monomers. The invention further provides the nanoparticles, the initiation monomers, and propagation monomers of formula P-L-N wherein P
is a moiety having a property selected from the group consisting of redox activity, optical activity, electrical activity and magnetic activity, and L and N are defined above. The invention also provides binding monomers of formula B-L-N, wherein B is a binding moiety that binds specifically to an analyte, and N and L are defined above. Finally, the invention provides methods and kits for detecting or quantitating an analyte.
CA002352362A 1998-11-30 1999-11-30 Nanoparticles with polymer shells Expired - Fee Related CA2352362C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11032798P 1998-11-30 1998-11-30
US60/110,327 1998-11-30
PCT/US1999/028387 WO2000033079A1 (en) 1998-11-30 1999-11-30 Nanoparticles with polymer shells

Publications (2)

Publication Number Publication Date
CA2352362A1 true CA2352362A1 (en) 2000-06-08
CA2352362C CA2352362C (en) 2009-02-17

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA002352362A Expired - Fee Related CA2352362C (en) 1998-11-30 1999-11-30 Nanoparticles with polymer shells

Country Status (9)

Country Link
US (1) US7115688B1 (en)
EP (2) EP1135682B1 (en)
JP (1) JP3650582B2 (en)
CN (2) CN1182395C (en)
AT (1) ATE366932T1 (en)
AU (1) AU768535B2 (en)
CA (1) CA2352362C (en)
DE (1) DE69936534T2 (en)
WO (1) WO2000033079A1 (en)

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CA2352362C (en) 2009-02-17
CN1607390A (en) 2005-04-20
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CN1182395C (en) 2004-12-29
EP1135682B1 (en) 2007-07-11
AU1928600A (en) 2000-06-19
US7115688B1 (en) 2006-10-03
CN100406890C (en) 2008-07-30
AU768535B2 (en) 2003-12-18
ATE366932T1 (en) 2007-08-15
JP2002531830A (en) 2002-09-24

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