CA2307623A1 - Serpentine electrophoresis channel with self-correcting bends - Google Patents

Serpentine electrophoresis channel with self-correcting bends Download PDF

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Publication number
CA2307623A1
CA2307623A1 CA002307623A CA2307623A CA2307623A1 CA 2307623 A1 CA2307623 A1 CA 2307623A1 CA 002307623 A CA002307623 A CA 002307623A CA 2307623 A CA2307623 A CA 2307623A CA 2307623 A1 CA2307623 A1 CA 2307623A1
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CA
Canada
Prior art keywords
alpha
channel
angle
self
curved
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002307623A
Other languages
French (fr)
Other versions
CA2307623C (en
Inventor
Eric S. Nordman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
The Perkin-Elmer Corporation
Eric S. Nordman
Pe Corporation (Ny)
Applera Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Perkin-Elmer Corporation, Eric S. Nordman, Pe Corporation (Ny), Applera Corporation filed Critical The Perkin-Elmer Corporation
Publication of CA2307623A1 publication Critical patent/CA2307623A1/en
Application granted granted Critical
Publication of CA2307623C publication Critical patent/CA2307623C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N2030/285—Control of physical parameters of the fluid carrier electrically driven carrier
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6095—Micromachined or nanomachined, e.g. micro- or nanosize
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S366/00—Agitating
    • Y10S366/03—Micromixers: variable geometry from the pathway influences mixing/agitation of non-laminar fluid flow

Abstract

A serpentine electrophoresis channel, e.g., for a microchip format, is disclosed. The channel includes pairs of linear segments, e.g., parallel or right-angle segments, each joined by an angled channel region having a first curved channel portion subtending an angle .alpha. f>.alpha.
where .alpha. is the angle between segments in a pair, and a second curved channel portion subtending an angle .alpha. s = .alpha. f-.alpha.. The angles and cross sections of the two channel portions are such that .delta.t f, the time differential of analyte migration at inner and outer tracks in the first curved portion is equal to .delta.t s, the time differential of analyte migration at outer and inner tracks in the second curved portion, respectively.
CA002307623A 1997-11-12 1998-11-12 Serpentine electrophoresis channel with self-correcting bends Expired - Fee Related CA2307623C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6510097P 1997-11-12 1997-11-12
US60/065,100 1997-11-12
PCT/US1998/024202 WO1999024828A1 (en) 1997-11-12 1998-11-12 Serpentine electrophoresis channel with self-correcting bends

Publications (2)

Publication Number Publication Date
CA2307623A1 true CA2307623A1 (en) 1999-05-20
CA2307623C CA2307623C (en) 2004-03-16

Family

ID=22060347

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002307623A Expired - Fee Related CA2307623C (en) 1997-11-12 1998-11-12 Serpentine electrophoresis channel with self-correcting bends

Country Status (8)

Country Link
US (1) US6176991B1 (en)
EP (1) EP1031032B1 (en)
JP (1) JP2001523001A (en)
AT (1) ATE220456T1 (en)
AU (1) AU747940B2 (en)
CA (1) CA2307623C (en)
DE (1) DE69806499T2 (en)
WO (1) WO1999024828A1 (en)

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WO2004030802A2 (en) * 2002-10-03 2004-04-15 Kreido Laboratories Apparatus for transfer of heat energy between a body surface and heat transfer fluid
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WO2004113898A1 (en) * 2003-05-19 2004-12-29 Protasis Corporation Electrophoresis devices and methods for focusing charged analytes
US7435381B2 (en) * 2003-05-29 2008-10-14 Siemens Healthcare Diagnostics Inc. Packaging of microfluidic devices
US7160025B2 (en) * 2003-06-11 2007-01-09 Agency For Science, Technology And Research Micromixer apparatus and methods of using same
US20040265171A1 (en) * 2003-06-27 2004-12-30 Pugia Michael J. Method for uniform application of fluid into a reactive reagent area
US20080257754A1 (en) * 2003-06-27 2008-10-23 Pugia Michael J Method and apparatus for entry of specimens into a microfluidic device
US20040265172A1 (en) * 2003-06-27 2004-12-30 Pugia Michael J. Method and apparatus for entry and storage of specimens into a microfluidic device
JP2005024316A (en) * 2003-06-30 2005-01-27 Kyocera Corp Microchemical chip and manufacturing method therefor
US7347617B2 (en) * 2003-08-19 2008-03-25 Siemens Healthcare Diagnostics Inc. Mixing in microfluidic devices
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Also Published As

Publication number Publication date
DE69806499D1 (en) 2002-08-14
WO1999024828A1 (en) 1999-05-20
EP1031032A1 (en) 2000-08-30
CA2307623C (en) 2004-03-16
AU1458099A (en) 1999-05-31
DE69806499T2 (en) 2003-02-27
ATE220456T1 (en) 2002-07-15
JP2001523001A (en) 2001-11-20
AU747940B2 (en) 2002-05-30
EP1031032B1 (en) 2002-07-10
US6176991B1 (en) 2001-01-23

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