CA2294962A1 - Nucleic acid sequence analysis - Google Patents

Nucleic acid sequence analysis Download PDF

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Publication number
CA2294962A1
CA2294962A1 CA002294962A CA2294962A CA2294962A1 CA 2294962 A1 CA2294962 A1 CA 2294962A1 CA 002294962 A CA002294962 A CA 002294962A CA 2294962 A CA2294962 A CA 2294962A CA 2294962 A1 CA2294962 A1 CA 2294962A1
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Prior art keywords
group
blocking group
nucleotide
polymerase
blocking
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
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CA002294962A
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French (fr)
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CA2294962C (en
Inventor
Daniel Henry Densham
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Gen Probe Inc
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Individual
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26311958&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2294962(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GBGB9715942.0A external-priority patent/GB9715942D0/en
Priority claimed from GBGB9727103.5A external-priority patent/GB9727103D0/en
Application filed by Individual filed Critical Individual
Publication of CA2294962A1 publication Critical patent/CA2294962A1/en
Application granted granted Critical
Publication of CA2294962C publication Critical patent/CA2294962C/en
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Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6825Nucleic acid detection involving sensors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Abstract

The present invention relates to a method for determining the sequence of a polynucleotide, the method comprising the steps of: (i) reacting a target polynucleotide with a polymerase enzyme immobilised on a solid support, and the different nucleotides, under conditions sufficient for the polymerase reaction; and (ii) detecting the incorporation of a specific nucleotide complementary to the target polynucleotide, by measuring radiation.

Claims (29)

1. A method for sequencing a polynucleotide, comprising the steps of:

(i) reacting a target polynucleotide with a polymerase enzyme immobilised on a solid support, and the different nucleotides, under conditions sufficient for the polymerase reaction; and (ii) detecting an effect consequent on the incorporation of a specific nucleotide complementary to the target polynucleotide.
2. A method according to claim 1, wherein the effect in step (ii) is detected by measuring radiation.
3. A method according to claim 1 or claim 2, wherein steps (i) and (ii) are conducted with each of the different nucleotides in turn, until incorporation is detected, and then repeated.
4. A method according to claim 1 or claim 2, wherein step (i) is conducted with all the nucleotides present.
5. A method according to any preceding claim, wherein the nucleotides comprise a 3' blocking group which is removed after the polymerase reaction.
6. A method according to claim 5, wherein the blocking group can be selectively removed by pulsed monochromatic light.
7. A method according to claim 5 or claim 6, wherein the nucleotides comprise a further blocking group at the terminal phosphate group of the triphosphate chain, and the further blocking group is removed prior to the removal of the 3' blocking group.
8. A method according to claim 7, wherein the further blocking group can be selectively removed by pulsed monochromatic light under conditions different from those required to remove the 3' blocking group.
9. A method according to claim 8, wherein the further blocking group is removed by pulsing the monochromatic light for a duration different from that required to remove the 3' blocking group.
10. A method according to any preceding claim, wherein step (i) further comprises introducing a competitive inhibitor of the polymerise enzyme.
11. A method according to any preceding claim, wherein the target polynucleotide of step (i) is bound to the polymerise enzyme by a .beta.2 dimer complex.
12. A method according to any preceding claim, wherein the polymerise is E. coli DNA polymerise III or T7 polymerase.
13. A method according to any of claims 1 to 11, wherein the polymerase is Taq polymerase.
14. A method according to any of claims 1 to 11, wherein the polymerase is reverse transcriptase.
15. A method according to any preceding claim, wherein step (ii) comprises detection of a change in resonance signal over time.
16. A method according to any preceding claim, wherein the radiation is electromagnetic.
17. A method according to claim 16, wherein the electromagnetic radiation is in the infra-red spectrum.
18. A method according to any preceding claim, wherein step (ii) comprises using surface plasmon resonance.
19. A method according to claim 16, wherein the electromagnetic radiation is in the radio-frequency spectrum.
20. A method according to claim 19, wherein the incorporation of a nucleotide is detected using NMR.
21. A method according to any preceding claim, wherein the polynucleotide is DNA.
22. A sensor chip comprising a polymerase enzyme immobilised thereon.
23. A nucleotide comprising a blocking group at the 3' position and at the terminal phosphate group of the triphosphate chain, wherein the two blocking groups are removable by monochromatic light of different wavelengths.
24. A nucleotide according to claim 23, wherein the blocking groups are derived from a compound of the formula R1-[O-CO-]X

wherein R1 is a photolabile group and X is a leaving group.
25. A nucleotide according to claim 23 or claim 24, wherein the blocking group at the 3' position is an o-nitrobenzyloxycarbonyl group.
26. A nucleotide according to any of claims 23 to 25, wherein the blocking group at the terminal phosphate is an o-nitrobenzyl group.
27. A nucleotide according to any of claims 23 to 26, wherein the blocking group at the 3' position is a (4,5-dimethoxy-2-nitrobenzyl)oxycarbonyl group.
28. A nucleotide according to any of claims 23 to 27, wherein the blocking group at the terminal phosphate is a 1-(2-nitrophenyl)ethyl group.
29. An apparatus for sequencing a polynucleotide, comprising an optical sensor chip, a light source, an imaging device and a photodetector, wherein the sensor chip is as defined in claim 22.
CA2294962A 1997-07-28 1998-07-24 Nucleic acid sequence analysis Expired - Lifetime CA2294962C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB9715942.0A GB9715942D0 (en) 1997-07-28 1997-07-28
GB9715942.0 1997-07-28
GB9727103.5 1997-12-22
GBGB9727103.5A GB9727103D0 (en) 1997-12-22 1997-12-22
PCT/GB1998/002214 WO1999005315A2 (en) 1997-07-28 1998-07-24 Nucleic acid sequence analysis

Publications (2)

Publication Number Publication Date
CA2294962A1 true CA2294962A1 (en) 1999-02-04
CA2294962C CA2294962C (en) 2012-09-18

Family

ID=26311958

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2294962A Expired - Lifetime CA2294962C (en) 1997-07-28 1998-07-24 Nucleic acid sequence analysis

Country Status (19)

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US (4) US7008766B1 (en)
EP (4) EP2267164B1 (en)
JP (1) JP2001511358A (en)
KR (1) KR100664331B1 (en)
CN (1) CN1152140C (en)
AT (1) ATE225858T1 (en)
AU (1) AU735898B2 (en)
BR (1) BR9812270A (en)
CA (1) CA2294962C (en)
DE (1) DE69808661T3 (en)
DK (1) DK1017848T3 (en)
ES (1) ES2183394T5 (en)
HK (1) HK1031130A1 (en)
IL (1) IL133411A (en)
IS (1) IS5360A (en)
NZ (1) NZ502557A (en)
PT (1) PT1017848E (en)
RU (1) RU2198221C2 (en)
WO (1) WO1999005315A2 (en)

Families Citing this family (138)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9620209D0 (en) * 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
ATE273381T1 (en) 1997-02-12 2004-08-15 Eugene Y Chan METHOD FOR ANALYZING POLYMERS
EP2267164B1 (en) * 1997-07-28 2016-09-14 Gen-Probe Incorporated Nucleic acid sequence analysis
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
AU2180200A (en) * 1998-12-14 2000-07-03 Li-Cor Inc. A heterogeneous assay for pyrophosphate detection
ATE397092T1 (en) * 1999-03-10 2008-06-15 Asm Scient Inc METHOD FOR DIRECT SEQUENCING OF NUCLEIC ACIDS
AU2005201777B2 (en) * 1999-03-10 2007-08-02 Asm Scientific, Inc. A Method for Direct Nucleic Acid Sequencing
GB9907812D0 (en) * 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
GB9907813D0 (en) * 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
US6756492B1 (en) 1999-04-08 2004-06-29 Deutsches Krebsforschungszentrum Stiftund Des Offentlichen Rechts Nucleoside derivatives with photo-unstable protective groups
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
EP1681357A3 (en) * 1999-05-19 2006-07-26 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
AU7086800A (en) * 1999-08-30 2001-03-26 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The High speed parallel molecular nucleic acid sequencing
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
GB9923644D0 (en) * 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
US6908736B1 (en) 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
EP1252172A2 (en) * 1999-12-21 2002-10-30 VBC-Genomics Bioscience Research GmbH Compound comprising a peptide moiety and an organo-silane moiety
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
GB0016473D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
AU8288101A (en) 2000-07-07 2002-01-21 Visigen Biotechnologies Inc Real-time sequence determination
JP2004510433A (en) 2000-10-06 2004-04-08 ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク Massively parallel methods for decoding DNA and RNA
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
EP1356120A2 (en) * 2001-01-30 2003-10-29 Solexa Ltd. Arrayed polynucleotides and their use in genome analysis
GB0112238D0 (en) 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
US7118907B2 (en) 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
US6613523B2 (en) * 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US6852492B2 (en) * 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6956114B2 (en) 2001-10-30 2005-10-18 '454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
AU2003259350A1 (en) 2002-08-23 2004-03-11 Solexa Limited Modified nucleotides for polynucleotide sequencing
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
JP2004347621A (en) * 2003-04-24 2004-12-09 Dainippon Printing Co Ltd Transmission type screen
GB0316553D0 (en) * 2003-07-15 2003-08-20 Densham Daniel Method
EP1644524A1 (en) * 2003-07-15 2006-04-12 Daniel Henry Densham Measurement of a polynucleotide amplification reaction
GB0317343D0 (en) * 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7977048B2 (en) 2004-01-13 2011-07-12 Pathogenetix, Inc. Detection and quantification of analytes in solution using polymers
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
US7981604B2 (en) 2004-02-19 2011-07-19 California Institute Of Technology Methods and kits for analyzing polynucleotide sequences
PL1818405T3 (en) 2004-04-09 2015-11-30 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants
GB0413082D0 (en) * 2004-06-11 2004-07-14 Medical Biosystems Ltd Method
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
EP1790202A4 (en) 2004-09-17 2013-02-20 Pacific Biosciences California Apparatus and method for analysis of molecules
US7274458B2 (en) 2005-03-07 2007-09-25 3M Innovative Properties Company Thermoplastic film having metallic nanoparticle coating
US7666494B2 (en) 2005-05-04 2010-02-23 3M Innovative Properties Company Microporous article having metallic nanoparticle coating
EP1901057A1 (en) * 2005-07-07 2008-03-19 Sony Corporation Substance information acquisition method and substance information measurement device using evanescent light, and base arrangement decision method and device
JP2009501095A (en) 2005-07-14 2009-01-15 スリーエム イノベイティブ プロパティズ カンパニー Water-soluble polymer substrate with metallic nanoparticle coating
US7805081B2 (en) 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
HUE031692T2 (en) 2005-09-16 2017-07-28 Monsanto Technology Llc Methods for genetic control of insect infestations in plants and compositions thereof
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US7763423B2 (en) 2005-09-30 2010-07-27 Pacific Biosciences Of California, Inc. Substrates having low density reactive groups for monitoring enzyme activity
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
WO2007070542A2 (en) 2005-12-12 2007-06-21 The Government Of The United States Of America As Represented By The Secretary Of The Deptartment Ofhealth And Human Services Nanoprobes for detection or modification of molecules
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
US7692783B2 (en) * 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7995202B2 (en) * 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7715001B2 (en) 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
US7563574B2 (en) 2006-03-31 2009-07-21 Pacific Biosciences Of California, Inc. Methods, systems and compositions for monitoring enzyme activity and applications thereof
US8471230B2 (en) 2006-09-01 2013-06-25 Pacific Biosciences Of California, Inc. Waveguide substrates and optical systems and methods of use thereof
US8207509B2 (en) 2006-09-01 2012-06-26 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
CA2662521C (en) 2006-09-01 2016-08-09 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US20080076123A1 (en) * 2006-09-27 2008-03-27 Helicos Biosciences Corporation Polymerase variants for DNA sequencing
US20080080059A1 (en) * 2006-09-28 2008-04-03 Pacific Biosciences Of California, Inc. Modular optical components and systems incorporating same
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US8551704B2 (en) 2007-02-16 2013-10-08 Pacific Biosciences Of California, Inc. Controllable strand scission of mini circle DNA
US20100167413A1 (en) * 2007-05-10 2010-07-01 Paul Lundquist Methods and systems for analyzing fluorescent materials with reduced autofluorescence
US20080277595A1 (en) * 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
DE102007022915A1 (en) * 2007-05-14 2008-11-20 Stiftung Caesar Center Of Advanced European Studies And Research Method for analyzing polymerization of analyte in fluid, particularly organic monomer, involves overflowing reaction medium before beginning of analyzing sensor surface
AU2008282862B2 (en) 2007-07-26 2014-07-31 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
WO2009045344A2 (en) 2007-09-28 2009-04-09 Pacific Biosciences Of California, Inc. Error-free amplification of dna for clonal sequencing
US7960116B2 (en) 2007-09-28 2011-06-14 Pacific Biosciences Of California, Inc. Nucleic acid sequencing methods and systems
US20110014611A1 (en) 2007-10-19 2011-01-20 Jingyue Ju Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequences by synthesis
DK2725107T3 (en) 2007-10-19 2019-01-02 Univ Columbia DNA Sequencing with Non-Fluorescent Nucleotide Reversible Terminators and ddNTPs Modified by Split Label and Nucleic Acid comprising Inosine with Reversible Terminators
EP2245444A4 (en) 2008-01-10 2014-04-16 Pacific Biosciences California Methods and systems for analysis of fluorescent reactions with modulated excitation
CA2715385A1 (en) 2008-02-12 2009-08-20 Pacific Biosciences Of California, Inc. Compositions and methods for use in analytical reactions
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8236499B2 (en) 2008-03-28 2012-08-07 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
MX2010010600A (en) 2008-03-28 2011-03-30 Pacific Biosciences California Inc Compositions and methods for nucleic acid sequencing.
US8143030B2 (en) 2008-09-24 2012-03-27 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8198023B2 (en) 2008-08-05 2012-06-12 Pacific Biosciences Of California, Inc. Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase
WO2010027497A2 (en) 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc Preparations, compositions, and methods for nucleic acid sequencing
US8481264B2 (en) 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
US8921046B2 (en) 2008-09-19 2014-12-30 Pacific Biosciences Of California, Inc. Nucleic acid sequence analysis
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2010059206A2 (en) 2008-11-19 2010-05-27 Pacific Biosciences Of California, Inc. Modular nucleotide compositions and uses therefor
US8370079B2 (en) 2008-11-20 2013-02-05 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
US8993230B2 (en) 2008-12-04 2015-03-31 Pacific Biosciences of Californ, Inc. Asynchronous sequencing of biological polymers
US9175341B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
US9175338B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
US20230148447A9 (en) 2008-12-11 2023-05-11 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
CA2760155A1 (en) 2009-04-27 2010-11-11 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
US8518643B2 (en) 2010-02-04 2013-08-27 Pacific Biosciences Of California, Inc. Method to improve single molecule analyses
EP2933629B1 (en) 2010-02-19 2019-04-10 Pacific Biosciences Of California, Inc. System for measuring analytical reactions comprising a socket for an optode array chip
WO2011116120A2 (en) * 2010-03-16 2011-09-22 Life Technologies Corporation Method and apparatus for addressable flow cells in single molecule sequencing
US8318094B1 (en) 2010-06-18 2012-11-27 Pacific Biosciences Of California, Inc. Substrate analysis systems
US8834847B2 (en) 2010-08-12 2014-09-16 Pacific Biosciences Of California, Inc. Photodamage mitigation compounds and systems
US8465922B2 (en) 2010-08-26 2013-06-18 Pacific Biosciences Of California, Inc. Methods and systems for monitoring reactions
WO2012129242A2 (en) 2011-03-23 2012-09-27 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
WO2012138973A2 (en) 2011-04-06 2012-10-11 The University Of Chicago COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-METHYLCYTOSINE (5mC)
US20150031024A1 (en) * 2011-07-30 2015-01-29 The Regents Of The University Of California Enzymatic preparation of 10 base to 50 kb double-strand dna reagent for sequencing with a nanopore-polymerase sequencing device
WO2013056241A2 (en) 2011-10-14 2013-04-18 Pacific Biosciences Of California, Inc. Real-time redox sequencing
US9267917B2 (en) 2011-11-04 2016-02-23 Pacific Biosciences Of California, Inc. Nanopores in zero mode waveguides
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
US9175348B2 (en) 2012-04-24 2015-11-03 Pacific Biosciences Of California, Inc. Identification of 5-methyl-C in nucleic acid templates
JP6333297B2 (en) 2013-03-15 2018-05-30 イルミナ ケンブリッジ リミテッド Modified nucleoside or modified nucleotide
US9416414B2 (en) 2013-10-24 2016-08-16 Pacific Biosciences Of California, Inc. Delaying real-time sequencing
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
US10731211B2 (en) 2015-11-18 2020-08-04 Pacific Biosciences Of California, Inc. Methods and compositions for loading of polymerase complexes
EP3449012B1 (en) 2016-04-29 2020-12-23 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
WO2018034780A1 (en) 2016-08-15 2018-02-22 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
CA3033741A1 (en) 2016-08-15 2018-02-22 Omniome, Inc. Method and system for sequencing nucleic acids
CA3048415C (en) 2016-12-30 2023-02-28 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
WO2018136487A1 (en) 2017-01-20 2018-07-26 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
US10415029B2 (en) 2017-01-20 2019-09-17 Omniome, Inc. Allele-specific capture of nucleic acids
US9932631B1 (en) 2017-09-11 2018-04-03 Omniome, Inc. Genotyping by polymerase binding
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
AU2018353136B2 (en) 2017-10-19 2022-05-19 Pacific Biosciences Of California, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
US11078534B2 (en) * 2017-11-27 2021-08-03 Roche Molecular Systems, Inc. Photocleavable nucleotide reagents with high stability
KR20210095796A (en) 2018-12-21 2021-08-03 일루미나, 인코포레이티드 sensing systems
AU2021379079A1 (en) * 2020-11-16 2023-01-05 Illumina Cambridge Limited Incorporation and imaging mixes

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3728032A (en) * 1971-10-13 1973-04-17 H Noll Flow cell for spectroscopic analysis of density gradients
GB2102005B (en) * 1981-07-23 1984-11-21 Mta Koezponti Kemiai Kutato In Process for synthesis of polydeoxynucleotides
US5064754A (en) * 1984-12-14 1991-11-12 Mills Randell L Genomic sequencing method
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
EP0425563B1 (en) 1988-07-20 1996-05-15 David Segev Process for amplifying and detecting nucleic acid sequences
SE462454B (en) * 1988-11-10 1990-06-25 Pharmacia Ab METHOD FOR USE IN BIOSENSORS
GB2225339A (en) * 1988-11-15 1990-05-30 Aligena Ag Separating electrically charged macromolecular compounds by forced-flow membrane electrophoresis
GB8910880D0 (en) 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
CA2097708A1 (en) 1990-12-06 1992-06-07 Stephen P. A. Fodor Very large scale immobilized polymer synthesis
DE4104076C2 (en) * 1991-02-11 1994-03-31 Bruker Analytische Messtechnik Device for nuclear magnetic resonance spectrometric measurement of chemical and / or physical reaction processes
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
GB9119735D0 (en) * 1991-09-16 1991-10-30 Secr Defence Gene probe biosensor method
US5583026A (en) * 1994-08-31 1996-12-10 Cornell Research Foundation, Inc. Process for reconstituting the polymerase III* and other subassemblies of E. coli DNA polymerase III holoenzyme from peptide subunits
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
SE9201984D0 (en) 1992-06-29 1992-06-29 Pharmacia Biosensor Ab IMPROVEMENT IN OPTICAL ASSAYS
US5360714A (en) * 1992-08-28 1994-11-01 Fox Chase Cancer Center Hepadnavirus polymerase gene product having RNA-dependent DNA priming and reverse transcriptase activities and methods of measuring the activities thereof
WO1994010128A1 (en) * 1992-11-02 1994-05-11 Affymax Technologies N.V. Novel photoreactive protecting groups
DE69430909T2 (en) 1993-03-19 2003-02-27 Sequenom Inc DNA SEQUENCE DETERMINATION BY MASS SPECTROMETRY ON THE WAY OF DEGRADATION WITH EXONUCLEASE
WO1995006138A1 (en) 1993-08-25 1995-03-02 The Regents Of The University Of California Microscopic method for detecting micromotions
US5485277A (en) * 1994-07-26 1996-01-16 Physical Optics Corporation Surface plasmon resonance sensor and methods for the utilization thereof
US5801042A (en) * 1994-08-18 1998-09-01 The Trustees Of Columbia University In The City Of New York Unique associated Kaposi's sarcoma virus sequences and uses thereof
US5635608A (en) * 1994-11-08 1997-06-03 Molecular Probes, Inc. α-carboxy caged compounds
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
US5753439A (en) * 1995-05-19 1998-05-19 Trustees Of Boston University Nucleic acid detection methods
US6331692B1 (en) * 1996-10-12 2001-12-18 Volker Krause Diode laser, laser optics, device for laser treatment of a workpiece, process for a laser treatment of workpiece
CA2282418A1 (en) 1997-03-05 1998-09-11 Scriptgen Pharmaceuticals, Inc. Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids
US6159687A (en) * 1997-03-18 2000-12-12 Novo Nordisk A/S Methods for generating recombined polynucleotides
EP2267164B1 (en) * 1997-07-28 2016-09-14 Gen-Probe Incorporated Nucleic acid sequence analysis
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) * 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
GB9907813D0 (en) * 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6908736B1 (en) * 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
GB9923644D0 (en) * 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
GB0112238D0 (en) * 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
GB0317343D0 (en) * 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
GB0413082D0 (en) * 2004-06-11 2004-07-14 Medical Biosystems Ltd Method
CN104093487B (en) * 2012-02-03 2017-06-09 安捷伦科技有限公司 Micromachined flow cell with separate stream pipe

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