CA2292787A1 - Nucleic acid primers and probes for detecting hiv-1 and hiv-2 - Google Patents

Nucleic acid primers and probes for detecting hiv-1 and hiv-2 Download PDF

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Publication number
CA2292787A1
CA2292787A1 CA002292787A CA2292787A CA2292787A1 CA 2292787 A1 CA2292787 A1 CA 2292787A1 CA 002292787 A CA002292787 A CA 002292787A CA 2292787 A CA2292787 A CA 2292787A CA 2292787 A1 CA2292787 A1 CA 2292787A1
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CA
Canada
Prior art keywords
primer
nos
seq
probe set
probe
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Granted
Application number
CA002292787A
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French (fr)
Other versions
CA2292787C (en
Inventor
Paul E. Kroeger
Klara Abravaya
Claudia A. Cygan Esping
Jacek J. Gorzowski
Robert J. Hoenle
Jennifer J. Moore
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Abbott Molecular Inc
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Individual
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Filing date
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Publication of CA2292787A1 publication Critical patent/CA2292787A1/en
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Publication of CA2292787C publication Critical patent/CA2292787C/en
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Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D

Abstract

Provided herein are primer/probe sets useful for detecting HIV (i.e. either or both HIV 1 or HIV 2) in a test sample. The primer/probe sets can be employed according to nucleic acid amplification procedures including PCR or RT PCR. The primer/probe sets can also be provided in the form of a kit with other reagents for performing a nucleic acid amplification reaction.

Claims (9)

1. A primer/probe set for detecting HIV 1 or HIV 2 selected from the primer/probe sets consisting of: primer/probe set 1 (SEQ. ID.
NOs. 2, 3 and 4); primer/probe set 2 (SEQ. ID. NOs. 5, 6 and 7);
primer/probe set 3 (SEQ. ID. NOs. 8, 9, 10 and 11); prime/probe set 4 (SEQ. ID. NOs. 12, 13 and 14); primer/probe set 5 (SEQ. ID. NOs. 16, 17 and 18); primer/probe set 6 (SEQ. ID. NOs. 19, 20 and 21 or 22);
primer/probe set 7 (SEQ. ID. NOs. 23, 20 and 21); and primer/probe set 8 (SEQ. ID. NOs. 16, 24 and 18).
2. A method of detecting HIV in a test sample, said method comprises the steps of:
(a) forming a reaction mixture comprising nucleic acid amplification reagents, at least one primer/probe set of claim 1, and a test sample containing an HIV target sequence;
(b) subjecting the mixture to amplification conditions to generate at least one copy of a nucleic acid sequence complementary to the target sequence;
(c) hybridizing the probe to the nucleic acid sequence complementary to the target sequence, so as to form a hybrid comprising the probe and the nucleic acid sequence complementary to the target sequence; and (d) detecting the hybrid as an indication of the presence of HIV in the test sample.
3. The method of claim 2 wherein said amplification reagents include an enzyme having reverse transcriptase activity and said method further comprises the step, after step (a) and prior to step (b), of forming a cDNA from an HIV genome.
4. The method of claim 2 wherein said at least one primer/probe set is selected from the primer/probe sets consisting of:
primer/probe set 1 (SEQ. ID. NOs. 2, 3 and 4); primer/probe set 2 (SEQ.
ID. NOs. 5, 6 and 7); primer/probe set 3 (SEQ. ID. NOs. 8, 9, 10 and 11 );
and primer/probe set 4 (SEQ. ID. NOs. 12, 13 and 14).
5. The method of claim 2 wherein said at least one primer/probe set is selected from the primer/probes sets consisting of:
primer/probe set 5 (SEQ. ID. NOs. 16, 17 and 18); primer/probe set 6 (SEQ. ID. NOs. 19, 20 and 21 or 22); primer/probe set 7 (SEGO. ID. NOs. 23, 20 and 21); and primer/probe set 8 (SEQ. ID. NOs. 16, 24 and 18).
6. The method of claim 2 wherein step (b) is repeated between and 100 times
7. A kit comprising:
a) at least one primer/probe set selected from the group consisting of: primer/probe set 1 (SEQ. ID. NOs. 2, 3 and 4), primer/probe set 2 (SEQ. ID. NOs. 5, 6 and 7), primer/probe set 3 (SEQ.
ID. NOs. 8, 9, 10 and 11), primer/probe set 4 (SEQ. ID. NOs. 12, 13 and 14), primer/probe set 5 (SEQ. ID. NOs. 16, 17 and 18), primer/probe set 6 (SEQ. ID. NOs. 19, 20 and 21 or 22), primer/probe set 7 (SEQ. ID. NOs.
23, 20 and 21), and primer/probe set 8 (SEQ. ID. NOs. 16, 24 and 18); and b) amplification reagents.
8. The kit of claim 7 wherein said amplification reagents comprise an enzyme having reverse transcriptase activity.
9. The kit of claim 7 wherein said kit comprises at least two primer/probe sets wherein (a) one of the at least two primer/probe sets is selected from the group consisting of primer/probe set 1 (SEQ. ID. NOs. 2, 3 and 4), primer/probe set 2 (SEQ. ID. NOs. 5, 6 and and 7), primer/probe set 3 (SEQ. ID. NOs. 8, 9, 10 and 11 ), primer/probe set 4 (SEQ. ID. NOs. 12, 13 and 14); and (b) one of the at least two primer/probe sets is selected from the group consisting of primer/probe set 5 (SEQ. ID. NOs. 16, 17 and 18), primer/probe set 6 (SEQ. ID. NOs. 19, 20, 21 and 22), primer/probe set 7 (SEQ. ID. NOs. 23, 20, 21 and 22), and primer/probe set 8 (SEQ. ID. NOs. 16, 24 and 18).
CA002292787A 1997-06-16 1998-06-04 Nucleic acid primers and probes for detecting hiv-1 and hiv-2 Expired - Fee Related CA2292787C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/876,546 US5962665A (en) 1997-06-16 1997-06-16 Nucleic acid primers and probes for detecting HIV-1 and HIV-2
US08/876,546 1997-06-16
PCT/US1998/011652 WO1998058086A2 (en) 1997-06-16 1998-06-04 Nucleic acid primers and probes for detecting hiv-1 and hiv-2

Publications (2)

Publication Number Publication Date
CA2292787A1 true CA2292787A1 (en) 1998-12-23
CA2292787C CA2292787C (en) 2009-11-24

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA002292787A Expired - Fee Related CA2292787C (en) 1997-06-16 1998-06-04 Nucleic acid primers and probes for detecting hiv-1 and hiv-2

Country Status (8)

Country Link
US (2) US5962665A (en)
EP (2) EP2067868B1 (en)
JP (1) JP3744548B2 (en)
AT (1) ATE428809T1 (en)
CA (1) CA2292787C (en)
DE (1) DE69840743D1 (en)
ES (2) ES2324125T3 (en)
WO (1) WO1998058086A2 (en)

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Also Published As

Publication number Publication date
JP3744548B2 (en) 2006-02-15
EP0991782B1 (en) 2009-04-15
ATE428809T1 (en) 2009-05-15
EP0991782A2 (en) 2000-04-12
EP2067868B1 (en) 2013-09-18
ES2445495T3 (en) 2014-03-03
WO1998058086A2 (en) 1998-12-23
US5962665A (en) 1999-10-05
ES2324125T3 (en) 2009-07-30
EP2067868A3 (en) 2009-09-23
EP2067868A2 (en) 2009-06-10
US6232455B1 (en) 2001-05-15
CA2292787C (en) 2009-11-24
DE69840743D1 (en) 2009-05-28
WO1998058086A3 (en) 1999-03-11
JP2000512160A (en) 2000-09-19

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