CA2287776A1 - Regulation of quinolate phosphoribosyl transferase expression - Google Patents

Regulation of quinolate phosphoribosyl transferase expression Download PDF

Info

Publication number
CA2287776A1
CA2287776A1 CA002287776A CA2287776A CA2287776A1 CA 2287776 A1 CA2287776 A1 CA 2287776A1 CA 002287776 A CA002287776 A CA 002287776A CA 2287776 A CA2287776 A CA 2287776A CA 2287776 A1 CA2287776 A1 CA 2287776A1
Authority
CA
Canada
Prior art keywords
plant
dna
phosphoribosyl transferase
quinolate phosphoribosyl
promoter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002287776A
Other languages
French (fr)
Other versions
CA2287776C (en
Inventor
Mark A. Conkling
Nandini Mendu
Wen Song
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
North Carolina State University
Original Assignee
North Carolina State University
Mark A. Conkling
Nandini Mendu
Wen Song
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21960004&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2287776(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by North Carolina State University, Mark A. Conkling, Nandini Mendu, Wen Song filed Critical North Carolina State University
Priority to CA002484366A priority Critical patent/CA2484366A1/en
Publication of CA2287776A1 publication Critical patent/CA2287776A1/en
Application granted granted Critical
Publication of CA2287776C publication Critical patent/CA2287776C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Manufacture Of Tobacco Products (AREA)

Abstract

DNA encoding a plant quinolate phosphoribosyl transferase (QPRTase) enzyme, and constructs comprising such DNA are provided. Methods of altering quinolate phosphoribosyl transferase expression are provided.

Claims (44)

1. An isolated DNA molecule comprising a sequence selected from the group consisting of:
(a) SEQ ID NO:1;
(b) DNA sequences which encode an enzyme having SEQ ID
N0:2;
(c) DNA sequences which hybridize to isolated DNA of (a) or (b) above and which encode a quinolate phosphoribosyl transferase enzyme; and (d) DNA sequences which differ from the DNA of (a), (b) or (c) above due to the degeneracy of the genetic code.
2. A DNA construct comprising an expression cassette, which construct comprises, in the 5' to 3' direction, a promoter operable in a plant cell and a DNA segment according to claim 1 positioned downstream from said promoter and operatively associated therewith.
3. A DNA construct comprising an expression cassette, which construct comprises, in the 5' to 3' direction, a plant promoter and a DNA
segment according to claim 1 positioned downstream from said promoter and operatively associated therewith, said DNA segment in antisense orientation.
4. A DNA construct comprising, in the 5' to 3' direction, a promoter operable in a plant cell and DNA encoding a plant quinolate phosphoribosyl transferase, said DNA operably associated with said promoter.
5. A DNA construct comprising, in the 5' to 3' direction, a promoter operable in a plant cell and DNA encoding a plant quinolate phosphoribosyl transferase, said DNA in antisense orientation and operably associated with said promoter.
6. A DNA construct according to claim 2, 3, 4 or 5, which promoter is constitutively active in plant cells.
7. A DNA construct according to claim 2, 3, 4 or 5 wherein said promoter is selectively active in plant root tissue cells.
8. A DNA construct according to claim 2, 3, 4 or 5, wherein said promoter is selectively active in plant root cortex tissue cells.
9. A DNA construct according to claim 2, 3, 4 or 5, wherein said construct further comprises a plasmid.
10. A DNA construct according to claim 2, 3, 4 or 5 carried by a plant transformation vector.
11. A DNA construct according to claim 2, 3, 4 or 5 carried by a plant transformation vector, which plant transformation vector is an Agrobacterium tumefaciens vector.
12. A plant cell containing a DNA construct according to claim 2, 3, 4 or 5.
13. A transgenic plant comprising plant cells according to claim 12.
14. A peptide having SEQ ID NO:2.
15. A peptide encoded by a DNA sequence selected from the group consisting of:
(a) SEQ ID NO:1;

(b) DNA sequences which hybridize to isolated DNA of (a) above and which encode a quinolate phosphoribosyl transferase enzyme; and (c) DNA sequences which differ from the DNA of (a) or (b) above due to the degeneracy of the genetic code.
16. A method of making a transgenic plant cell having reduced quinolate phosphoribosyl transferase (QPRTase) expression, said method comprising:
providing a plant cell of a type known to express quinolate phosphoribosyl transferase;
providing an exogenous DNA construct, which construct comprises, in the 5' to 3' direction, a promoter operable in a plant cell and DNA comprising a portion of a sequence encoding quinolate phosphoribosyl transferase mRNA, said DNA operably associated with said promoter; and transforming said plant cell with said DNA construct to produce transformed cells, said plant cell having reduced expression of QPRTase compared to an untransformed cell.
17. The method of claim 16, wherein said DNA comprising a portion of a sequence encoding quinolate phosphoribosyl transferase mRNA is in antisense orientation.
18. The method of claim 16, wherein said DNA comprising a portion of a sequence encoding quinolate phosphoribosyl transferase mRNA is in sense orientation.
19. The method of claim 16, wherein said plant cell is Nicotiana tabacum.
20. The method of claim 16, further comprising regenerating a plant from said transformed plant cell.
21. A method according to claim 16, wherein said promoter is constitutively active.
22. A method according to claim 16, wherein said promoter is selectively active in plant root tissue cells.
23. A method according to claim 16, wherein said promoter is selectively active in plant root cortex tissue cells.
24. A method according to claim 16, wherein said transforming step is carried out by bombarding said plant cell with microparticles carrying said DNA
construct.
25. A method according to claim 16 wherein said transforming step is carried out by infecting said plant cell with an Agrobacterium tumefaciens containing a Ti plasmid carrying said DNA construct.
26. A method of producing transgenic tobacco seeds, comprising collecting seed from a transgenic tobacco plant produced by the method of claim 19.
27. The method according to claim 16, wherein said exogenous DNA
sequence is complementary to said quinolate phosphoribosyl transferase messenger RNA (QPRT mRNA) expressed in said plant cell in a region selected from:
(a) the 5'-untranslated sequence of said QPRT mRNA;
(b) the 3'-untranslated sequence of said QPRT mRNA; and (c) the translated region of said QPRT mRNA.
28. The method according to claim 16, wherein said exogenous DNA
sequence is complementary to at least 15 nucleotides of said quinolate phosphoribosyl transferase messenger RNA expressed in said plant cell
29. The method according to claim16, wherein said exogenous DNA
sequence is complementary to at least 200 nucleotides of said quinolate phosphoribosyl transferase messenger RNA expressed in said plant cell
30. The method according to claim 16, wherein said exogenous DNA
sequence comprises a quinolate phosphoribosyl transferase encoding sequence selected from the DNA sequences of Claim 1.
31. A transgenic plant of the species Nicotiana having reduced quinolate phosphoribosyl transferase (QPRTase) expression relative to a non-transformed control plant, said transgenic plant comprising transgenic plant cells containing:
an exogenous DNA construct comprising, in the 5' to 3' direction, a promoter operable in said plant cell and DNA comprising a segment of a DNA
sequence that encodes a plant quinolate phosphoribosyl transferase mRNA, said DNA operably associated with said promoter;
said plant exhibiting reduced QPRTase expression compared to a non-transformed control plant.
32. The method of claim 31, wherein said segment of DNA
comprising a segment of a DNA sequence encoding quinolate phosphoribosyl transferase mRNA is in antisense orientation.
33. The method of claim 31, wherein said segment of DNA comprising a segment of a DNA sequence encoding quinolate phosphoribosyl transferase mRNA is in sense orientation.
34. A transgenic plant of the species Nicotiana having reduced quinolate phosphoribosyl transferase (QPRTase) expression relative to a non-transformed control plant, wherein said transgenic plant is a progeny of a plant according to claim 31.
35. Seeds of a transgenic plant of the species Nicotiana having reduced quinolate phosphoribosyl transferase (QPRTase) expression relative to a non-transformed control plant, wherein said transgenic plant is a plant according to claim 31 or a progeny thereof.
36. A crop comprising a plurality of plants according to claim 31 planted together in an agricultural field.
37. A method for reducing expression of a quinolate phosphoribosyl transferase gene in a plant cell, said method comprising:
growing a plant cell transformed to contain exogenous DNA, wherein a transcribed strand of said exogenous DNA is complementary to quinolate phosphoribosyl transferase mRNA endogenous to said cell, whereby transcription of said complementary strand reduces expression of said quinolate phosphoribosyl gene.
38. A method of producing a tobacco plant having decreased levels of nicotine in leaves of said tobacco plant, said method comprising:
growing a tobacco plant, or progeny plants thereof, wherein said plant comprises cells containing a DNA construct comprising a transcriptional initiation region functional in said plant and an exogenous DNA sequence operably joined to said transcriptional initiation region, wherein a transcribed strand of said DNA
sequence is complementary to endogenous quinolate phosphoribosyl transferase messenger RNA in said cells.
39. A method of making a transgenic plant cell having increased quinolate phosphoribosyl transferase (QPRTase) expression, said method comprising:
providing a plant cell of a type known to express quinolate phosphoribosyl transferase;
providing an exogenous DNA construct, which construct comprises, in the 5' to 3' direction, a promoter operable in a plant cell and a DNA sequence encoding quinolate phosphoribosyl transferase, said DNA sequence operably associated with said promoter; and transforming said plant cell with said DNA construct to produce transformed cells, said plant cell having increased expression of QPRTase compared to an untransformed cell.
40. A transgenic plant of the species Nicotiana having increased quinolate phosphoribosyl transferase (QPRTase) expression relative to a non-transformed control plant, said transgenic plant comprising transgenic plant cells containing:
an exogenous DNA construct comprising, in the 5' to 3' direction, a promoter operable in said plant cell and a DNA sequence encoding a plant quinolate phosphoribosyl transferase, said DNA operably associated with said promoter;
said plant exhibiting increased QPRTase expression compared to a non-transformed control plant.
41. A transgenic plant of the species Nicotiana having increased quinolate phosphoribosyl transferase (QPRTase) expression relative to a non-transformed control plant, wherein said transgenic plant is a progeny of a plant according to claim 74.
42. A method for increasing expression of a quinolate phosphoribosyl transferase gene in a plant cell, said method comprising:

growing a plant cell transformed to contain exogenous DNA, wherein said exogenous DNA encodes quinolate phosphoribosyl transferase.
43. The method according to claim 83, wherein said transformed plant cell is obtained by a method comprising:
integrating into the genome of a host plant cell a construct comprising, in the direction of transcription, a promoter functional in said plant cell, a DNA
sequence encoding quinolate phosphoribosyl transferase functional in said cell, said DNA sequence operably associated with said promoter, and a transcriptional termination region functional in said cell, whereby a transformed plant cell is obtained.
44. A method of producing a tobacco plant having increased levels of nicotine in leaves of said tobacco plant, said method comprising:
growing a tobacco plant, or progeny plants thereof, wherein said plant comprises cells containing a DNA construct comprising a transcriptional initiation region functional in said plant and an exogenous DNA sequence operably joined to said transcriptional initiation region, wherein said DNA sequence encodes quinolate phosphoribosyl transferase functional in said cells.
CA2287776A 1997-06-12 1998-06-10 Regulation of quinolate phosphoribosyl transferase expression Expired - Lifetime CA2287776C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002484366A CA2484366A1 (en) 1997-06-12 1998-06-10 Regulation of quinolate phosphoribosyl transferase expression

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4947197P 1997-06-12 1997-06-12
US60/049,471 1997-06-12
PCT/US1998/011893 WO1998056923A1 (en) 1997-06-12 1998-06-10 Regulation of quinolate phosphoribosyl transferase expression

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002484366A Division CA2484366A1 (en) 1997-06-12 1998-06-10 Regulation of quinolate phosphoribosyl transferase expression

Publications (2)

Publication Number Publication Date
CA2287776A1 true CA2287776A1 (en) 1998-12-17
CA2287776C CA2287776C (en) 2012-04-03

Family

ID=21960004

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2287776A Expired - Lifetime CA2287776C (en) 1997-06-12 1998-06-10 Regulation of quinolate phosphoribosyl transferase expression
CA002484366A Abandoned CA2484366A1 (en) 1997-06-12 1998-06-10 Regulation of quinolate phosphoribosyl transferase expression

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA002484366A Abandoned CA2484366A1 (en) 1997-06-12 1998-06-10 Regulation of quinolate phosphoribosyl transferase expression

Country Status (38)

Country Link
US (9) US6586661B1 (en)
EP (3) EP1457563B1 (en)
JP (4) JP4095678B2 (en)
KR (1) KR100365969B1 (en)
CN (2) CN100482799C (en)
AP (1) AP1169A (en)
AT (1) ATE262039T1 (en)
AU (1) AU748514B2 (en)
BG (1) BG64319B1 (en)
BR (1) BRPI9810870B1 (en)
CA (2) CA2287776C (en)
CU (1) CU23174A3 (en)
CZ (1) CZ297379B6 (en)
DE (2) DE69822463T2 (en)
DK (1) DK0991766T3 (en)
EA (2) EA004652B1 (en)
EE (1) EE04595B1 (en)
ES (1) ES2154624T3 (en)
GE (1) GEP20032871B (en)
GR (1) GR20010300003T1 (en)
HK (3) HK1027379A1 (en)
HU (1) HU225888B1 (en)
ID (1) ID29311A (en)
IL (3) IL132184A0 (en)
LT (1) LT4706B (en)
LV (1) LV12507B (en)
NO (1) NO324872B1 (en)
NZ (1) NZ500963A (en)
OA (1) OA11219A (en)
PL (1) PL201266B1 (en)
PT (1) PT991766E (en)
RO (1) RO121968B1 (en)
RS (1) RS49677B (en)
SI (1) SI20215B (en)
SK (1) SK161899A3 (en)
TR (1) TR199902728T2 (en)
UA (1) UA72187C2 (en)
WO (1) WO1998056923A1 (en)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6586661B1 (en) * 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
US20100251425A9 (en) * 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
DE19926216A1 (en) * 1999-06-09 2001-02-22 Metallgesellschaft Ag Process for producing barium sulfate, barium sulfate and use of barium sulfate
KR20030029885A (en) * 2000-08-30 2003-04-16 노쓰 캐롤라이나 스테이트 유니버시티 Transgenic plants containing molecular decoys that alter protein content therein
AU2002230691A1 (en) * 2000-11-07 2002-05-21 North Carolina State University Putrescine-n-methyltransferase promoter
DE10055870A1 (en) * 2000-11-10 2002-05-29 Degussa New nucleotide sequences coding for the nadC gene
CN1288248C (en) * 2001-06-06 2006-12-06 二十二世纪有限责任公司 Tobacco biomass utilization
NZ530238A (en) * 2001-06-08 2004-08-27 Vector Tobacco Ltd Modifying nicotine and nitrosamine levels in tobacco
WO2005103296A2 (en) * 2004-03-30 2005-11-03 Vector Tobacco, Ltd. Global gene expression analysis of human bronchial epithelial cells exposed to cigarette smoke, smoke condensates, or components thereof
US20060157072A1 (en) * 2001-06-08 2006-07-20 Anthony Albino Method of reducing the harmful effects of orally or transdermally delivered nicotine
US6730832B1 (en) 2001-09-10 2004-05-04 Luis Mayan Dominguez High threonine producing lines of Nicotiana tobacum and methods for producing
JP2005508648A (en) * 2001-11-09 2005-04-07 ベクター・タバコ・インコーポレーテッド Composition and method for mentholization of charcoal filtered cigarettes
AU2002357903A1 (en) * 2001-12-19 2003-07-09 Vector Tobacco Inc. Method and composition for mentholation of cigarettes
WO2003053176A2 (en) * 2001-12-19 2003-07-03 Vector Tobacco Inc. Method and compositions for imparting cooling effect to tobacco products
EA200401332A1 (en) * 2002-04-09 2005-02-24 Вектор Тобакко Лтд. TOBACCO WITH REDUCED CONTENTS OF NICOTINE AND NITROZAMINS
WO2005018307A1 (en) * 2003-08-19 2005-03-03 22Nd Century Limited, Llc Reduced-exposure tobacco products
US7920906B2 (en) 2005-03-10 2011-04-05 Dexcom, Inc. System and methods for processing analyte sensor data for sensor calibration
EP1684603A2 (en) * 2003-10-02 2006-08-02 Vector Tobacco Ltd. Tobacco product labeling system
US9247900B2 (en) 2004-07-13 2016-02-02 Dexcom, Inc. Analyte sensor
US7538071B2 (en) 2003-11-21 2009-05-26 Carl Berger Methods of reducing the nicotine content of tobacco plants and tobacco plants obtained thereby
US8792955B2 (en) 2004-05-03 2014-07-29 Dexcom, Inc. Transcutaneous analyte sensor
US7654956B2 (en) 2004-07-13 2010-02-02 Dexcom, Inc. Transcutaneous analyte sensor
AU2012203977B2 (en) * 2005-02-28 2015-01-29 22Nd Century Limited, Llc Reducing levels of nicotinic alkaloids in plants
AU2006233359B2 (en) 2005-02-28 2012-04-05 22Nd Century Limited, Llc Reducing levels of nicotinic alkaloids in plants
WO2006124448A2 (en) 2005-05-11 2006-11-23 Vector Tobacco Inc. Reduced risk tobacco products and methods of making same
FR2889003A1 (en) * 2005-07-22 2007-01-26 St Microelectronics Sa AUTOMATIC ADAPTATION OF A VIDEO SOURCE TO A RECEIVER
US8410341B2 (en) 2006-06-19 2013-04-02 National Research Council Of Canada Nucleic acid encoding N-methylputrescine oxidase and uses thereof
PL2792750T3 (en) 2006-09-13 2020-02-28 22Nd Century Limited, Llc Increasing levels of nicotinic alkaloids
US9551003B2 (en) 2006-09-13 2017-01-24 22Nd Century Limited, Llc Increasing levels of nicotinic alkaloids in plants
US9102948B2 (en) 2006-11-17 2015-08-11 22Nd Century Limited, Llc Regulating alkaloids
US9106606B1 (en) 2007-02-05 2015-08-11 F5 Networks, Inc. Method, intermediate device and computer program code for maintaining persistency
US8822757B2 (en) 2007-05-25 2014-09-02 National Research Council Of Canada Nucleic acid sequences encoding transcription factors regulating nicotine alkaloid biosynthesis and their use in modifying plant metabolism
US20100206317A1 (en) * 2007-09-28 2010-08-19 Vector Tobacco, Inc. Reduced risk tobacco products and use thereof
WO2009074325A1 (en) 2007-12-13 2009-06-18 Philip Morris Products S.A. Transgenic plants modified for reduced cadmium transport, derivative products, and related methods
WO2011062298A1 (en) 2009-11-19 2011-05-26 国立大学法人岡山大学 System for increasing gene expression, and vector supporting said system
US8535210B2 (en) 2009-12-11 2013-09-17 Terumo Bct, Inc. System for blood separation with shielded extraction port and optical control
EP2537929B1 (en) 2010-02-17 2017-04-12 Japan Tobacco, Inc. Plant component regulation factor, and use thereof
US9862923B2 (en) 2010-03-26 2018-01-09 Philip Morris Usa Inc. Cultured tobacco cells as a matrix for consumable products
EP2565265A1 (en) 2011-09-02 2013-03-06 Philip Morris Products S.A. Isopropylmalate synthase from Nicotiana tabacum and methods and uses thereof
US8716571B2 (en) 2011-09-21 2014-05-06 Reynolds Technologies, Inc. Tobacco having reduced amounts of amino acids and methods for producing such lines
US9137958B2 (en) 2012-02-08 2015-09-22 Reynolds Technologies, Inc. Tobacco having altered amounts of environmental contaminants
CA2894955C (en) * 2012-12-21 2023-10-31 Philip Morris Products S.A. Tobacco specific nitrosamine reduction in plants
CN103173488B (en) * 2013-03-14 2014-09-03 浙江省农业科学院 Method for quickly screening paddy transgenes by novel fusion tag
US10375910B2 (en) 2013-03-15 2019-08-13 Altria Client Services Llc Development of tobacco varieties with no or significantly reduced anatabine content
BR112017023719A2 (en) * 2015-05-05 2018-07-17 Univ North Carolina State tobacco plant, plant part and / or plant cell, seed, plant culture, tobacco product, method for reducing pon and / or nnk, method of producing a plant and method for producing a tobacco product
CN116250483B (en) 2015-06-26 2024-01-30 奥驰亚客户服务公司 Compositions and methods for producing tobacco plants and articles having altered alkaloid content
EP3480314A1 (en) 2017-11-03 2019-05-08 Philip Morris Products S.A. Regulation of alkaloid content
US20200035118A1 (en) 2018-07-27 2020-01-30 Joseph Pandolfino Methods and products to facilitate smokers switching to a tobacco heating product or e-cigarettes
US10897925B2 (en) 2018-07-27 2021-01-26 Joseph Pandolfino Articles and formulations for smoking products and vaporizers
WO2021072288A1 (en) * 2019-10-10 2021-04-15 Altria Client Services Llc Compositions and methods based on qpt engineering for producing tobacco plants and products having altered alkaloid levels
CN113528537A (en) * 2021-08-11 2021-10-22 云南省烟草农业科学研究院 NtQPT2 gene mutant for reducing nicotine content in tobacco leaves and application thereof

Family Cites Families (178)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US38123A (en) * 1863-04-07 Improvement in harvesters
US254285A (en) * 1882-02-28 David w
US299541A (en) * 1884-06-03 heae-n
US819751A (en) * 1905-01-04 1906-05-08 Giuseppe Gianoli Process of charging silk.
US2479526A (en) * 1940-12-11 1949-08-16 Wurton Machine Company Apparatus for curing green tobacco
US2728803A (en) * 1951-12-28 1955-12-27 Standard Oil Co Separation of ethylbenzene from other c8 aromatic hydrocarbons by extraction with hf-bf3
US2728603A (en) 1954-12-13 1955-12-27 James H Stagg Lawn and garden sprinkler
DE1917552U (en) 1964-01-31 1965-06-10 Fritz Tautenhahn FLAT ORNAMENTAL LATTICE WORK.
US3693631A (en) 1971-04-28 1972-09-26 Reynolds Leasing Corp Tobacco expansion process
US3840025A (en) * 1972-08-14 1974-10-08 Industrial Nucleonics Corp Tobacco moisture control system and method
US3905123A (en) * 1973-10-15 1975-09-16 Industrial Nucleonics Corp Method and apparatus for controlling a tobacco dryer
US4319587A (en) * 1975-06-09 1982-03-16 Irving S. Moser Smoking article
DE2531285C2 (en) * 1975-07-12 1982-10-28 Deutsche Benkert Gmbh & Co Kg, 4690 Herne Filter cigarette
US4192323A (en) * 1977-09-21 1980-03-11 Gas-Fired Products, Inc. Apparatus and method for automatically controlling curing conditions in a tobacco curing barn
US4557280A (en) 1978-06-15 1985-12-10 Brown & Williamson Tobacco Corporation Process for reduction of nitrate and nicotine content of tobacco by microbial treatment
US4243056A (en) * 1979-01-12 1981-01-06 Philip Morris Incorporated Method for uniform incorporation of additives into tobacco
FR2478958A1 (en) * 1980-04-01 1981-10-02 Decoufle INK SUPPLY DEVICE FOR PRINTING APPARATUSES FOR CIGARETTE-MAKING MACHINES
US4499911A (en) * 1980-12-09 1985-02-19 Johnson William H Energy efficient curing and drying system
GB2092149B (en) * 1981-02-03 1985-01-03 Searle & Co Imidazoline hydrazone and hydrazine derivatives production thereof and use in medicine
US4459355A (en) * 1982-07-12 1984-07-10 International Paper Company Method for transforming plant cells
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
NL8203963A (en) * 1982-10-14 1984-05-01 Naarden International Nv METHOD FOR AROMATIZING DRY VEGETABLE MATERIAL
ATE52800T1 (en) 1983-01-13 1990-06-15 Max Planck Gesellschaft METHOD OF INTRODUCING EXPRESSIONABLE GENES INTO PLANT CELL GENOMES AND HYBRID TI PLASMID VECTORS CONTAINING AGROBACTERIUM STRAINS USABLE IN THIS METHOD.
US6051757A (en) 1983-01-14 2000-04-18 Washington University Regeneration of plants containing genetically engineered T-DNA
US6174724B1 (en) 1983-01-17 2001-01-16 Monsanto Company Chimeric genes suitable for expression in plant cells
EP0131623B2 (en) 1983-01-17 1999-07-28 Monsanto Company Chimeric genes suitable for expression in plant cells
US5034322A (en) 1983-01-17 1991-07-23 Monsanto Company Chimeric genes suitable for expression in plant cells
EP0131624B1 (en) 1983-01-17 1992-09-16 Monsanto Company Plasmids for transforming plant cells
US5352605A (en) 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
JPS60500795A (en) 1983-01-17 1985-05-30 モンサント　カンパニ− genetically transformed plants
NL8300699A (en) 1983-02-24 1984-09-17 Univ Leiden METHOD FOR BUILDING FOREIGN DNA INTO THE NAME OF DIABIC LOBAL PLANTS; METHOD FOR PRODUCING AGROBACTERIUM TUMEFACIENS BACTERIEN; STABLE COINTEGRATE PLASMIDS; PLANTS AND PLANT CELLS WITH CHANGED GENETIC PROPERTIES; PROCESS FOR PREPARING CHEMICAL AND / OR PHARMACEUTICAL PRODUCTS.
NL8300698A (en) 1983-02-24 1984-09-17 Univ Leiden METHOD FOR BUILDING FOREIGN DNA INTO THE NAME OF DIABIC LOBAL PLANTS; AGROBACTERIUM TUMEFACIENS BACTERIA AND METHOD FOR PRODUCTION THEREOF; PLANTS AND PLANT CELLS WITH CHANGED GENETIC PROPERTIES; PROCESS FOR PREPARING CHEMICAL AND / OR PHARMACEUTICAL PRODUCTS.
US4751348A (en) 1983-07-16 1988-06-14 Cold Spring Harbor Laboratory Nicotiana plants with both altered polyamine levels and flower structures and method for obtaining these plants
US4766855A (en) * 1983-07-20 1988-08-30 Cummins Engine Co., Inc. Plasma jet ignition apparatus
US5208149A (en) 1983-10-20 1993-05-04 The Research Foundation Of State University Of New York Nucleic acid constructs containing stable stem and loop structures
US5190931A (en) 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US5272065A (en) 1983-10-20 1993-12-21 Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
EP0140308B2 (en) 1983-10-20 2001-10-17 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition utilizing mRNA interfering complementary RNA
US4885248A (en) 1984-02-15 1989-12-05 Lubrizol Genetics, Inc. Transfer vector
US4771002A (en) 1984-02-24 1988-09-13 Lubrizol Genetics, Inc. Transcription in plants and bacteria
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
NL8401780A (en) 1984-06-04 1986-01-02 Rijksuniversiteit Leiden En Pr METHOD FOR BUILDING FOREIGN DNA INTO THE GENOMES OF PLANTS
US5100792A (en) 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
US5036006A (en) 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
EP0189707B1 (en) 1984-12-28 1993-08-25 Plant Genetic Systems N.V. Recombinant dna which can be introduced into plant cells
US5753475A (en) 1985-01-17 1998-05-19 Calgene, Inc. Methods and compositions for regulated transcription and expression of heterologous genes
US6281410B1 (en) 1986-07-31 2001-08-28 Calgene Llc Methods and compositions for regulated transcription and expression of heterologous genes
US4943674A (en) 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
US6617496B1 (en) 1985-10-16 2003-09-09 Monsanto Company Effecting virus resistance in plants through the use of negative strand RNAs
NL8502948A (en) 1985-10-29 1987-05-18 Rijksuniversiteit Leiden En Pr METHOD FOR INCORPORATING "FOREIGN DNA" INTO THE NAME OF DICOTYLE PLANTS
US4795855A (en) * 1985-11-14 1989-01-03 Joanne Fillatti Transformation and foreign gene expression with woody species
GB8529851D0 (en) 1985-12-04 1986-01-15 Rothmans Of Pall Mall Linear layered cigarette
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
IL81737A (en) 1986-03-28 1992-11-15 Calgene Inc Regulation of gene expression in plant cells
US5453566A (en) 1986-03-28 1995-09-26 Calgene, Inc. Antisense regulation of gene expression in plant/cells
US4700725A (en) * 1986-04-17 1987-10-20 Philip Morris Incorporated Adjustable filter cigarette
US4699158A (en) * 1986-04-17 1987-10-13 Philip Morris Incorporated Adjustable filter cigarette with tactile indicator
DE3661587D1 (en) * 1986-04-23 1989-02-09 Reynolds Tobacco Gmbh Process for treating tobacco and similar organic materials
US4766911A (en) * 1986-06-23 1988-08-30 R. J. Reynolds Tobacco Company Method for tracing smoking articles
US4962028A (en) 1986-07-09 1990-10-09 Dna Plant Technology Corporation Plant promotors
US5229292A (en) 1986-07-28 1993-07-20 Stine Seed Farm, Inc. Biological control of insects using pseudomonas strains transformed with bacillus thuringiensis insect toxingene
EP0265556A1 (en) 1986-10-31 1988-05-04 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Stable binary agrobacterium vectors and their use
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5015580A (en) * 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US4835162A (en) * 1987-02-12 1989-05-30 Abood Leo G Agonists and antagonists to nicotine as smoking deterents
US4966916A (en) * 1987-02-12 1990-10-30 Abood Leo G Agonists and antagonists to nicotine as smoking deterrents
US5356799A (en) 1988-02-03 1994-10-18 Pioneer Hi-Bred International, Inc. Antisense gene systems of pollination control for hybrid seed production
US5922602A (en) 1988-02-26 1999-07-13 Biosource Technologies, Inc. Cytoplasmic inhibition of gene expression
US5179022A (en) 1988-02-29 1993-01-12 E. I. Du Pont De Nemours & Co. Biolistic apparatus for delivering substances into cells and tissues in a non-lethal manner
US4954442A (en) 1988-08-31 1990-09-04 Purdue Research Foundation Opine enhancement of vir gene induction
US5023179A (en) * 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
GB8827592D0 (en) 1988-11-25 1988-12-29 Taniguchi T Improvements in & relating to regulation of expression
US5223419A (en) * 1989-03-14 1993-06-29 The Rockefeller University Alteration of gene expression in plants
US4990607A (en) * 1989-03-14 1991-02-05 The Rockefeller University Alteration of gene expression in plants
US5034323A (en) 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5231020A (en) 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
GB8916213D0 (en) 1989-07-14 1989-08-31 Ici Plc Dna constructs,cells and plants derived therefrom
US6203976B1 (en) 1989-07-18 2001-03-20 Osi Pharmaceuticals, Inc. Methods of preparing compositions comprising chemicals capable of transcriptional modulation
US5776502A (en) 1989-07-18 1998-07-07 Oncogene Science, Inc. Methods of transcriptionally modulating gene expression
ATE238431T1 (en) * 1989-07-18 2003-05-15 Osi Pharm Inc METHOD OF ALTERING THE EXPRESSION OF GENES DURING TRANSCRIPTION AND DETECTING CHEMICAL SUBSTANCES THAT ACTION LIKE MODULATORS OF GENE EXPRESSION
US5580722A (en) 1989-07-18 1996-12-03 Oncogene Science, Inc. Methods of determining chemicals that modulate transcriptionally expression of genes associated with cardiovascular disease
US5665543A (en) 1989-07-18 1997-09-09 Oncogene Science, Inc. Method of discovering chemicals capable of functioning as gene expression modulators
US5501967A (en) 1989-07-26 1996-03-26 Mogen International, N.V./Rijksuniversiteit Te Leiden Process for the site-directed integration of DNA into the genome of plants
US5097025A (en) 1989-08-01 1992-03-17 The Rockefeller University Plant promoters
US5062434A (en) 1989-09-22 1991-11-05 Brown & Williamson Tobacco Corporation Cigarette paper
GB8923716D0 (en) 1989-10-20 1989-12-06 Ici Plc Dna,constructs,cells and plants derived therefrom
US5177308A (en) 1989-11-29 1993-01-05 Agracetus Insecticidal toxins in plants
EP0434616B1 (en) 1989-12-19 1995-11-15 Ciba-Geigy Ag Method and appliance for the genetic transformation of cells
JPH0673478B2 (en) * 1990-01-11 1994-09-21 旭化成工業株式会社 L-carnitine highly sensitive assay method and assay composition
CA2036935A1 (en) 1990-02-26 1991-08-27 Paul Christou Plant transformation process with early identification of germ line transformation events
US5109876A (en) * 1990-04-19 1992-05-05 R. J. Reynolds Tobacco Company Cigarette paper and cigarette incorporating same
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5260205A (en) 1990-11-14 1993-11-09 Philip Morris Incorporated Method of purifying putrescine N-methyltransferase from tobacco plant extract with a polyamine
US5668295A (en) 1990-11-14 1997-09-16 Philip Morris Incorporated Protein involved in nicotine synthesis, DNA encoding, and use of sense and antisense DNAs corresponding thereto to affect nicotine content in transgenic tobacco cells and plants
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5035252A (en) 1990-12-14 1991-07-30 Mondre Steven J Nicotine-containing dental floss
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
JPH04265569A (en) * 1991-02-19 1992-09-21 Canon Inc Sound signal recording device and reproducing device
CA2106386A1 (en) 1991-04-18 1992-10-19 Barbara C. F. Chu Oligodeoxynucleotides and oligonucleotides useful as decoys for proteins which selectively bind to defined dna sequences
GB9109063D0 (en) 1991-04-26 1991-06-12 Ici Plc Modification of lignin synthesis in plants
EP0823486A3 (en) 1991-06-27 2004-02-11 Genelabs Technologies, Inc. Method for inhibiting the binding of a dna-binding protein to duplex dna
US5994629A (en) 1991-08-28 1999-11-30 Novartis Ag Positive selection
GB9304200D0 (en) 1993-03-02 1993-04-21 Sandoz Ltd Improvements in or relating to organic compounds
WO1993005646A1 (en) * 1991-09-13 1993-04-01 Technology Management Services, S.A. Reduction of nicotine levels in tobacco
ATE201236T1 (en) 1992-02-26 2001-06-15 Zeneca Mogen B V AGROBACTERIUM STRAINS CAPABILITY FOR SITE-SPECIFIC RECOMMINATION
RU2148647C1 (en) * 1992-04-02 2000-05-10 Сембайозис Дженетикс Инк. Method of expression of interesting dna sequence in seed cell, dna-structure, expression cassette, isolated regulatory site of transcription and method of alternation of specific metabolism in seeds
US5780051A (en) * 1992-04-02 1998-07-14 Dynagen, Inc. Methods and articles of manufacture for nicotine cessation and monitoring nicotine use
US5626152A (en) * 1992-08-26 1997-05-06 Molins Plc Cigarette making machine
US5469871A (en) * 1992-09-17 1995-11-28 R. J. Reynolds Tobacco Company Cigarette and method of making same
BR9307547A (en) * 1992-11-27 1999-06-01 Voxel Process and apparatus for making a hologram
JPH08503853A (en) 1992-11-30 1996-04-30 チューア，ナム−ハイ Expression motifs that confer tissue- and development-specific expression in plants
IL108367A0 (en) 1993-01-27 1994-04-12 Hektoen Inst For Medical Resea Antisense polynzcleotide inhibition of human growth factor-sensitive cancer cells
ATE203772T1 (en) 1993-05-13 2001-08-15 Aventis Cropscience Nv MARKING GEN
WO1994028142A1 (en) * 1993-06-01 1994-12-08 Philip Morris Products Inc. Putrescine n-methyltransferase, recombinant dna molecules encoding putrescine n-methyltransferase, and transgenic tobacco plants with decreased alkaloid content
US5540242A (en) * 1993-07-07 1996-07-30 Brown & Williamson Tobacco Corporation Cigarette paper having reduced sidestream properties
US5377697A (en) * 1993-08-27 1995-01-03 Hoechst Celanese Corporation Cigarette filter test apparatus and associated method for measuring filter hot collapse and tobacco consumption
US5810020A (en) * 1993-09-07 1998-09-22 Osmotek, Inc. Process for removing nitrogen-containing anions and tobacco-specific nitrosamines from tobacco products
CA2155570C (en) 1993-12-08 2007-06-26 Toshihiko Komari Method for transforming plant and vector therefor
US5394894A (en) * 1994-02-22 1995-03-07 Zade; Ismail Y. Method and apparatus for elimination of smoking
US5858774A (en) 1994-05-12 1999-01-12 The Research Foundation Of State University Of New York Antisense DNA constructs for expression of hybrid MRNAs driven by inducible, tissue-specific promoters
ES2081257B1 (en) 1994-05-12 1996-07-16 Sagrera Jorge Martinez INSTALLATION AND PROCEDURE FOR CURING TOBACCO.
JP3235934B2 (en) 1994-08-04 2001-12-04 三菱レイヨン株式会社 Kanamycin resistance gene from Rhodococcus bacteria
IT1275697B1 (en) * 1994-12-20 1997-10-17 Olmo Giancarlo Dell METHOD FOR PRINTING HOLOGRAMS, KINOGRAMS, DIFFRACTION RETICLES OR MICRO-ENGRAVINGS DIRECTLY ON PAPER
WO1996029418A1 (en) 1995-03-22 1996-09-26 Novo Nordisk A/S Introduction of dna into bacillus strains by conjugation
KR100424844B1 (en) 1995-03-30 2004-07-05 다카라 홀딩즈 가부시키가이샤 Plant promoter and gene expression method using this promoter
DE69636997T2 (en) * 1995-05-12 2007-07-12 Anges MG Inc., Ibaraki HEALING AND PREVENTION OF AF-kappaB-CAUSED DISEASES
US5837876A (en) * 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
GB9517263D0 (en) 1995-08-23 1995-10-25 Cancer Res Campaign Tech Expression systems
US6051409A (en) 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
US6198827B1 (en) * 1995-12-26 2001-03-06 Rocktron Corporation 5-2-5 Matrix system
US5693512A (en) 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US5851804A (en) 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
US5713376A (en) 1996-05-13 1998-02-03 Berger; Carl Non-addictive tobacco products
US6022863A (en) 1996-05-21 2000-02-08 Yale University Regulation of gene expression
US5834236A (en) * 1996-06-27 1998-11-10 The Salk Institute For Biological Studies AATT repeat transcription enhancer element
USRE38123E1 (en) * 1996-06-28 2003-05-27 Regent Court Technologies, Llc. Tobacco products having reduced nitrosamine content
US6135121A (en) * 1996-06-28 2000-10-24 Regent Court Technologies Tobacco products having reduced nitrosamine content
US5845647A (en) * 1996-06-28 1998-12-08 Regent Court Technologies Tobacco and related products
US5803081A (en) * 1996-06-28 1998-09-08 Regent Court Technologies Tobacco and related products
US5830318A (en) 1996-10-25 1998-11-03 Schweitzer-Mauduit International, Inc. High opacity tipping paper
US5929306A (en) 1996-11-15 1999-07-27 University Of Kentucky Research Foundation KYRT1, a disarmed version of a highly tumorigenic Agrobacterium tumefaciens strain identified as Chry5
US6202649B1 (en) * 1996-12-02 2001-03-20 Regent Court Technologies Method of treating tobacco to reduce nitrosamine content, and products produced thereby
US6166032A (en) * 1997-02-07 2000-12-26 Synapse Pharmaceuticals International, Inc. Method for controlling tobacco use and alleviating withdrawal symptoms due to cessation of tobacco use
ES1036967Y (en) * 1997-04-15 1998-05-01 Techpack Espana S L PERFECTED CUP FOR LIPSTICK CASE.
US6163521A (en) * 1997-04-16 2000-12-19 Matsushita Electric Industrial Co., Ltd. Disk having read-only and read-write areas
US6020989A (en) * 1997-05-14 2000-02-01 Affinity Co., Ltd. Laminated bodies and windows using them
US6586661B1 (en) 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
US6020969A (en) * 1997-07-11 2000-02-01 Philip Morris Incorporated Cigarette making machine including band inspection
CA2248622A1 (en) 1997-09-23 1999-03-23 Joe Celeste Biolistic apparatus for delivering substances into cells and tissues
JP2001518520A (en) * 1997-10-03 2001-10-16 キャリー メディカル コーポレイション Composition for treating nicotine addiction comprising a nicotine receptor antagonist and an antidepressant or anxiolytic
US6077992A (en) * 1997-10-24 2000-06-20 E. I. Du Pont De Nemours And Company Binary viral expression system in plants
US6060310A (en) 1997-11-24 2000-05-09 The United States Of America As Represented By The Department Of Health And Human Services Transcription factor decoy and tumor growth inhibitor
US6153811A (en) * 1997-12-22 2000-11-28 Dekalb Genetics Corporation Method for reduction of transgene copy number
US6265538B1 (en) * 1998-02-27 2001-07-24 The Regents Of The University Of California Inhibitor of the inflammatory response induced by the TNFA and IL-1
JP3444191B2 (en) * 1998-03-31 2003-09-08 日本製紙株式会社 Transcription factors that regulate the phenylpropanoid biosynthetic pathway
ATE507299T1 (en) 1998-04-08 2011-05-15 Commw Scient Ind Res Org METHOD AND MEANS FOR OBTAINING ALTERED PHENOTYPES
US6136799A (en) 1998-04-08 2000-10-24 Abbott Laboratories Cosolvent formulations
US5938017A (en) * 1998-05-04 1999-08-17 Wik; Dennis O. Article for assisting persons to quit smoking and method for same
JP2002516869A (en) * 1998-06-05 2002-06-11 レジェンド コート テクノロジーズ Monoamine oxidase (MAO) inhibitors and uses thereof
US6271031B1 (en) 1998-08-12 2001-08-07 E.I. Du Pont De Nemours And Company Quinolinate metabolism enzymes
AU764100B2 (en) * 1998-10-01 2003-08-07 Pioneer Hi-Bred International, Inc. Method of plant transformation
DE19847767A1 (en) * 1998-10-16 2000-04-20 Decoufle Sarl Arrangement for supplying flowable printing ink to a printing unit for a cigarette paper strip
WO2000067558A1 (en) 1999-05-06 2000-11-16 Michael Timko Regulation of gene expression in tobacco for manipulation of plant growth and secondary metabolism
US6314964B1 (en) * 1999-09-15 2001-11-13 Schweitzer-Mauduit International, Inc. Cigarette paper containing carbon fibers for improved ash characteristics
EP1250447B1 (en) 1999-11-29 2011-12-21 Midwest Oilseeds, Inc. Methods, media and apparatus for the introduction of molecules into plant cells and bacteria using aerosol beams
WO2001051630A1 (en) 2000-01-07 2001-07-19 Baylor University Antisense compositions and methods
CA2403397A1 (en) 2000-03-16 2001-09-20 Genetica, Inc. Methods and compositions for rna interference
WO2001077350A2 (en) 2000-04-07 2001-10-18 Large Scale Biology Corporation Compositions and methods for inhibiting gene expression
KR20030029885A (en) * 2000-08-30 2003-04-16 노쓰 캐롤라이나 스테이트 유니버시티 Transgenic plants containing molecular decoys that alter protein content therein
AU2002230691A1 (en) * 2000-11-07 2002-05-21 North Carolina State University Putrescine-n-methyltransferase promoter
CN1288248C (en) 2001-06-06 2006-12-06 二十二世纪有限责任公司 Tobacco biomass utilization
NZ530238A (en) * 2001-06-08 2004-08-27 Vector Tobacco Ltd Modifying nicotine and nitrosamine levels in tobacco
US20060157072A1 (en) * 2001-06-08 2006-07-20 Anthony Albino Method of reducing the harmful effects of orally or transdermally delivered nicotine
US6557560B2 (en) * 2001-06-18 2003-05-06 Ctc Canada Inc. Cigarette making machine
AU2006233359B2 (en) 2005-02-28 2012-04-05 22Nd Century Limited, Llc Reducing levels of nicotinic alkaloids in plants
US8410341B2 (en) 2006-06-19 2013-04-02 National Research Council Of Canada Nucleic acid encoding N-methylputrescine oxidase and uses thereof
BRPI0717355B1 (en) 2006-10-13 2018-01-16 North Carolina State University A method for obtaining a transgenic nicotine plant, a method for obtaining a seed; RECOMBINANT NUCLEIC ACID CONSTRUCTION; NICOTINE CONVERSION REDUCTION METHOD IN NORNICOTINE IN A NICOTINE PLANT

Also Published As

Publication number Publication date
ID29311A (en) 2001-08-16
CA2484366A1 (en) 1998-12-17
KR20010013663A (en) 2001-02-26
EP0991766A1 (en) 2000-04-12
CA2287776C (en) 2012-04-03
CU23174A3 (en) 2006-09-22
CZ297379B6 (en) 2006-11-15
NO996169D0 (en) 1999-12-13
LT4706B (en) 2000-09-25
EP1457562B1 (en) 2012-08-08
HK1027379A1 (en) 2001-01-12
PT991766E (en) 2004-08-31
US7408098B2 (en) 2008-08-05
KR100365969B1 (en) 2002-12-26
NO996169L (en) 1999-12-13
CN100482799C (en) 2009-04-29
HK1069411A1 (en) 2005-05-20
YU64899A (en) 2004-12-31
PL337442A1 (en) 2000-08-14
US20060191035A1 (en) 2006-08-24
JP4459271B2 (en) 2010-04-28
EA200400186A1 (en) 2004-08-26
TR199902728T2 (en) 2000-09-21
DE69822463D1 (en) 2004-04-22
EP1457563B1 (en) 2012-08-08
ES2154624T1 (en) 2001-04-16
SI20215A (en) 2000-10-31
CN1304576C (en) 2007-03-14
PL201266B1 (en) 2009-03-31
BG64319B1 (en) 2004-09-30
RS49677B (en) 2007-11-15
US7304220B2 (en) 2007-12-04
US20040168211A1 (en) 2004-08-26
SK161899A3 (en) 2000-06-12
BRPI9810870B1 (en) 2021-06-01
EA009898B1 (en) 2008-04-28
GR20010300003T1 (en) 2001-06-29
EE04595B1 (en) 2006-02-15
US7795509B2 (en) 2010-09-14
SI20215B (en) 2007-04-30
IL192232A0 (en) 2008-12-29
HK1065066A1 (en) 2005-02-08
NZ500963A (en) 2001-09-28
LV12507A (en) 2000-06-20
AU748514B2 (en) 2002-06-06
RO121968B1 (en) 2008-09-30
WO1998056923A1 (en) 1998-12-17
US7645925B2 (en) 2010-01-12
HUP0003767A2 (en) 2001-02-28
BR9810870A (en) 2000-11-14
ATE262039T1 (en) 2004-04-15
US6586661B1 (en) 2003-07-01
IL132184A (en) 2010-11-30
JP2008131950A (en) 2008-06-12
HU225888B1 (en) 2007-11-28
EP1457562A1 (en) 2004-09-15
GEP20032871B (en) 2003-01-27
BG103886A (en) 2000-07-31
LV12507B (en) 2000-11-20
US7605308B2 (en) 2009-10-20
EA004652B1 (en) 2004-06-24
JP2009112316A (en) 2009-05-28
UA72187C2 (en) 2005-02-15
US7425670B2 (en) 2008-09-16
ES2154624T3 (en) 2004-11-01
DK0991766T3 (en) 2004-07-12
EP1457563A1 (en) 2004-09-15
EP0991766B1 (en) 2004-03-17
JP4288206B2 (en) 2009-07-01
US20060200872A1 (en) 2006-09-07
EE9900575A (en) 2000-08-15
JP2004290201A (en) 2004-10-21
DE991766T1 (en) 2002-04-04
CN1618972A (en) 2005-05-25
JP4095678B2 (en) 2008-06-04
US20030140366A1 (en) 2003-07-24
US20060191036A1 (en) 2006-08-24
NO324872B1 (en) 2007-12-17
AP1169A (en) 2003-06-30
OA11219A (en) 2003-07-17
IL132184A0 (en) 2001-03-19
JP2001522250A (en) 2001-11-13
HUP0003767A3 (en) 2002-10-28
US6423520B1 (en) 2002-07-23
US20020108151A1 (en) 2002-08-08
AU7829198A (en) 1998-12-30
CN1257542A (en) 2000-06-21
LT99142A (en) 2000-06-26
DE69822463T2 (en) 2005-01-20
US20070011774A1 (en) 2007-01-11
CZ367799A3 (en) 2000-04-12
AP9901680A0 (en) 1999-12-31
EA200000007A1 (en) 2000-06-26

Similar Documents

Publication Publication Date Title
CA2287776A1 (en) Regulation of quinolate phosphoribosyl transferase expression
AU2002300895B2 (en) Regulation of quinolate phosphoribosyl transferase expression
JP3054687B2 (en) L-galactonolactone dehydrogenase gene
AU2004203879B2 (en) Regulation of quinolate phosphoribosyl transferase expression
JP3735709B2 (en) Higher plant transgenic plants with improved productivity
MXPA99011625A (en) Regulation of quinolate phosphoribosyl transferase expression

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20180611