CA2287570A1 - Two-step hybridization and capture of a polynucleotide - Google Patents

Two-step hybridization and capture of a polynucleotide Download PDF

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Publication number
CA2287570A1
CA2287570A1 CA002287570A CA2287570A CA2287570A1 CA 2287570 A1 CA2287570 A1 CA 2287570A1 CA 002287570 A CA002287570 A CA 002287570A CA 2287570 A CA2287570 A CA 2287570A CA 2287570 A1 CA2287570 A1 CA 2287570A1
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CA
Canada
Prior art keywords
target polynucleotide
hybridization
capture
probe
capture probe
Prior art date
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Granted
Application number
CA002287570A
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French (fr)
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CA2287570C (en
Inventor
William G. Weisburg
Jay H. Shaw
Michael M. Becker
Mehrdad Majlessi
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Gen Probe Inc
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Individual
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Publication of CA2287570C publication Critical patent/CA2287570C/en
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Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Electrical Control Of Air Or Fuel Supplied To Internal-Combustion Engine (AREA)

Abstract

A method for capturing a target polynucleotide in a sample onto a solid support with an attached immobilized probe by using a capture probe and two different hybridization conditions, which preferably differ in temperature only, is disclosed. The two hybridization conditions control the order of hybridization, where the first hybridization condition allows the capture probe and the target polynucleotide to hybridize, and the second hybridization condition allows the capture probe and the immobilized probe to hybridize, to produce a complex of immobilized probe, capture probe and target polynucleotide. A method for determining the presence of a target polynucleotide in a sample by capturing a target polynucleotide, amplifying the captured target polynucleotide and detecting amplified sequences is also disclosed.
CA002287570A 1997-05-02 1998-05-01 Two-step hybridization and capture of a polynucleotide Expired - Lifetime CA2287570C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4543097P 1997-05-02 1997-05-02
US60/045,430 1997-05-02
PCT/US1998/008853 WO1998050583A1 (en) 1997-05-02 1998-05-01 Two-step hybridization and capture of a polynucleotide

Publications (2)

Publication Number Publication Date
CA2287570A1 true CA2287570A1 (en) 1998-11-12
CA2287570C CA2287570C (en) 2008-10-28

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ID=21937832

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002287570A Expired - Lifetime CA2287570C (en) 1997-05-02 1998-05-01 Two-step hybridization and capture of a polynucleotide

Country Status (11)

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US (3) US6110678A (en)
EP (1) EP0975807B1 (en)
JP (1) JP4222635B2 (en)
KR (1) KR20010012175A (en)
AT (1) ATE340868T1 (en)
AU (1) AU738708B2 (en)
CA (1) CA2287570C (en)
DE (1) DE69836012T2 (en)
DK (1) DK0975807T3 (en)
ES (1) ES2271994T3 (en)
WO (1) WO1998050583A1 (en)

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