CA2282497A1 - In vitro peptide or protein expression library - Google Patents

In vitro peptide or protein expression library Download PDF

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Publication number
CA2282497A1
CA2282497A1 CA002282497A CA2282497A CA2282497A1 CA 2282497 A1 CA2282497 A1 CA 2282497A1 CA 002282497 A CA002282497 A CA 002282497A CA 2282497 A CA2282497 A CA 2282497A CA 2282497 A1 CA2282497 A1 CA 2282497A1
Authority
CA
Canada
Prior art keywords
expression library
protein expression
library
vitro peptide
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002282497A
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French (fr)
Other versions
CA2282497C (en
Inventor
Bjorn H. Lindqvist
David Andrews
Elizabeth Haggard-Ljungquist
Morten Isaksen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Isogenica Ltd
Original Assignee
Actinova Limited
Bjorn H. Lindqvist
David Andrews
Elizabeth Haggard-Ljungquist
Morten Isaksen
Isogenica Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Actinova Limited, Bjorn H. Lindqvist, David Andrews, Elizabeth Haggard-Ljungquist, Morten Isaksen, Isogenica Limited filed Critical Actinova Limited
Publication of CA2282497A1 publication Critical patent/CA2282497A1/en
Application granted granted Critical
Publication of CA2282497C publication Critical patent/CA2282497C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10111—Myoviridae
    • C12N2795/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

The present invention provides a method of producing an in vitro peptide or protein expression library which displays a diverse population of peptides or proteins, wherein the peptides or proteins are specifically associated with the DNA encoding them through covalent protein:DNA binding expecially through the use of cis-acting proteins, the library itself, DNA molecules encoding the library and uses of the expression library.
CA002282497A 1997-02-18 1998-02-18 In vitro peptide or protein expression library Expired - Fee Related CA2282497C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9703369.0A GB9703369D0 (en) 1997-02-18 1997-02-18 Process
GB9703369.0 1997-02-18
PCT/GB1998/000518 WO1998037186A1 (en) 1997-02-18 1998-02-18 In vitro peptide or protein expression library

Publications (2)

Publication Number Publication Date
CA2282497A1 true CA2282497A1 (en) 1998-08-27
CA2282497C CA2282497C (en) 2007-04-24

Family

ID=10807887

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002282497A Expired - Fee Related CA2282497C (en) 1997-02-18 1998-02-18 In vitro peptide or protein expression library

Country Status (14)

Country Link
US (1) US7416847B1 (en)
EP (1) EP1007654B1 (en)
JP (1) JP4644321B2 (en)
AT (1) ATE297462T1 (en)
AU (1) AU744791B2 (en)
CA (1) CA2282497C (en)
DE (2) DE69830497T2 (en)
DK (1) DK1007654T3 (en)
ES (1) ES2241117T3 (en)
GB (1) GB9703369D0 (en)
IL (2) IL131435A0 (en)
NO (1) NO322653B1 (en)
PT (1) PT1007654E (en)
WO (1) WO1998037186A1 (en)

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