CA2264581A1 - Characterising dna - Google Patents

Characterising dna

Info

Publication number
CA2264581A1
CA2264581A1 CA002264581A CA2264581A CA2264581A1 CA 2264581 A1 CA2264581 A1 CA 2264581A1 CA 002264581 A CA002264581 A CA 002264581A CA 2264581 A CA2264581 A CA 2264581A CA 2264581 A1 CA2264581 A1 CA 2264581A1
Authority
CA
Canada
Prior art keywords
sub
fragment
sticky end
sampling
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002264581A
Other languages
French (fr)
Other versions
CA2264581C (en
Inventor
Gunter Schmidt
Andrew Hugin Thompson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xzillion GmbH and Co KG
Original Assignee
Brax Genomics Limited
Gunter Schmidt
Andrew Hugin Thompson
Brax Group Limited
Aventis Research & Technologies Gmbh & Co. Kg
Xzillion Gmbh & Co. Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brax Genomics Limited, Gunter Schmidt, Andrew Hugin Thompson, Brax Group Limited, Aventis Research & Technologies Gmbh & Co. Kg, Xzillion Gmbh & Co. Kg filed Critical Brax Genomics Limited
Publication of CA2264581A1 publication Critical patent/CA2264581A1/en
Application granted granted Critical
Publication of CA2264581C publication Critical patent/CA2264581C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

A method for characterising cDNA, which comprises: (a) cutting a sample comprising a population of one or more cDNAs or isolated fragments thereof, each having a strand complementary to the 3' poly-A terminus of an mRNA and bearing a tail, with a first sampling endonuclease at a first sampling site of known displacement from a reference site proximal to the tail to generate from each cDNA or isolated fragment thereof a first and second sub-fragment, each comprising a sticky end sequence of predetermined length and unknown sequence, the first sub-fragment bearing the tail; (b) sorting either the first or second sub-fragments into sub-populations according to their sticky end sequence and recording the sticky end sequence of each sub-population as the first sticky end; (c) cutting the sub-fragments in each sub-population with a second sampling endonuclease, which is the same as or different from the first sampling endonuclease, at a second sampling site of known displacement from the first sampling site to generate from each sub-fragment a further sub-fragment comprising a second sticky end sequence of predetermined length and unknown sequence; and (d) determining each second sticky end sequence; wherein the aggregate length of the first and second sticky end sequences of each sub-fragment is from 6 to 10; and wherein the sequences and relative positions of the reference site and first and second sticky ends characterise the or each cDNA.
CA002264581A 1996-09-05 1997-09-05 Characterising dna Expired - Fee Related CA2264581C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9618544.2A GB9618544D0 (en) 1996-09-05 1996-09-05 Characterising DNA
GB9618544.2 1996-09-05
PCT/GB1997/002403 WO1998010095A1 (en) 1996-09-05 1997-09-05 Characterising dna

Publications (2)

Publication Number Publication Date
CA2264581A1 true CA2264581A1 (en) 1998-03-12
CA2264581C CA2264581C (en) 2003-11-11

Family

ID=10799468

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002264581A Expired - Fee Related CA2264581C (en) 1996-09-05 1997-09-05 Characterising dna

Country Status (12)

Country Link
US (1) US6225077B1 (en)
EP (1) EP0927267B1 (en)
JP (1) JP3863189B2 (en)
CN (1) CN1118581C (en)
AT (1) ATE215994T1 (en)
AU (1) AU721861B2 (en)
CA (1) CA2264581C (en)
DE (1) DE69711895T2 (en)
GB (1) GB9618544D0 (en)
IL (1) IL128809A (en)
NZ (1) NZ334426A (en)
WO (1) WO1998010095A1 (en)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE374836T1 (en) * 1997-01-15 2007-10-15 Xzillion Gmbh & Co Kg MASS MARKED HYBRIDIZATION PROBE
US6699668B1 (en) 1997-01-15 2004-03-02 Xzillion Gmbh & Co. Mass label linked hybridisation probes
GB9707980D0 (en) 1997-04-21 1997-06-11 Brax Genomics Ltd Characterising DNA
US6054276A (en) 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
US6136537A (en) * 1998-02-23 2000-10-24 Macevicz; Stephen C. Gene expression analysis
US7399844B2 (en) * 1998-07-09 2008-07-15 Agilent Technologies, Inc. Method and reagents for analyzing the nucleotide sequence of nucleic acids
AU1478200A (en) * 1998-11-16 2000-06-05 Genelabs Technologies, Inc. Method for measuring target polynucleotides and novel asthma biomolecules
US8367322B2 (en) 1999-01-06 2013-02-05 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
CA2356697C (en) * 1999-01-06 2010-06-22 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
EP1196554B1 (en) * 1999-03-18 2009-02-25 Complete Genomics AS Methods of cloning and producing fragment chains with readable information content
EP1182252A4 (en) * 1999-05-10 2002-10-09 Toyo Kohan Co Ltd Methods for constructing dna library and support carrying dna library immobilized thereon
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
DE19925862A1 (en) * 1999-06-07 2000-12-14 Diavir Gmbh Process for the synthesis of DNA fragments
AU7712400A (en) * 1999-09-23 2001-04-24 Gene Logic, Inc. Indexing populations
JP3668075B2 (en) * 1999-10-12 2005-07-06 光夫 板倉 Suspension system for determining genetic material sequence, method for determining genetic material sequence using the suspension system, and SNPs high-speed scoring method using the suspension system
AU1812801A (en) * 1999-12-06 2001-06-12 Pioneer Hi-Bred International, Inc. Short shared nucleotide sequences
JP2001204463A (en) * 2000-01-27 2001-07-31 Toyo Kohan Co Ltd Support for immobilizing nucleotide
WO2001061036A2 (en) * 2000-02-17 2001-08-23 Complete Genomics As A method of mapping restriction endonuclease cleavage sites
US6468749B1 (en) * 2000-03-30 2002-10-22 Quark Biotech, Inc. Sequence-dependent gene sorting techniques
DE10060827A1 (en) * 2000-12-07 2002-06-13 Basf Lynx Bioscience Ag Methods of coding hybridization probes
WO2002059357A2 (en) * 2001-01-24 2002-08-01 Genomic Expression Aps Assay and kit for analyzing gene expression
US20030143612A1 (en) * 2001-07-18 2003-07-31 Pointilliste, Inc. Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
US7189509B2 (en) * 2001-08-16 2007-03-13 Zhifeng Shao Analysis of gene expression profiles using sequential hybridization
ATE414767T1 (en) 2001-11-22 2008-12-15 Sloning Biotechnology Gmbh NUCLEIC ACID LINKERS AND THEIR USE IN GENE SYNTHESIS
US7291460B2 (en) * 2002-05-31 2007-11-06 Verenium Corporation Multiplexed systems for nucleic acid sequencing
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
FR2852605B1 (en) * 2003-03-18 2012-11-30 Commissariat Energie Atomique PROCESS FOR PREPARING DNA FRAGMENTS AND ITS APPLICATIONS
US7642064B2 (en) * 2003-06-24 2010-01-05 Ventana Medical Systems, Inc. Enzyme-catalyzed metal deposition for the enhanced detection of analytes of interest
US20040265883A1 (en) * 2003-06-27 2004-12-30 Biocept, Inc. mRNA expression analysis
DE602004029326D1 (en) 2004-01-23 2010-11-11 Sloning Biotechnology Gmbh Enzymatic production of nucleic acid molecules
FR2890859B1 (en) 2005-09-21 2012-12-21 Oreal DOUBLE-STRANDED RNA OLIGONUCLEOTIDE INHIBITING TYROSINASE EXPRESSION
US9115352B2 (en) 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
CN101942509B (en) * 2010-06-10 2013-02-27 广州医学院第一附属医院 Method for detecting common/identical DNA sequence in two unknown DNA fragments
EP2732038B1 (en) 2011-07-15 2018-09-05 The General Hospital Corporation Methods of transcription activator like effector assembly
US9890364B2 (en) 2012-05-29 2018-02-13 The General Hospital Corporation TAL-Tet1 fusion proteins and methods of use thereof
US20150267176A1 (en) 2012-10-12 2015-09-24 The General Hospital Corporation Transcription activator-like effector (tale) - lysine-specific demethylase 1 (lsd1) fusion proteins
CA2900338A1 (en) 2013-02-07 2014-08-14 The General Hospital Corporation Tale transcriptional activators
ITUB20155686A1 (en) * 2015-11-18 2017-05-18 Fondazione St Italiano Tecnologia Nucleic acid detection procedure and related kit.
TWI699435B (en) * 2016-07-01 2020-07-21 體學生物科技股份有限公司 Methods of constructing circular template and detecting dna molecules

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
US5695934A (en) * 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
DE69621507T2 (en) * 1995-03-28 2003-01-09 Japan Science & Tech Corp Method for molecular indexing of genes using restriction enzymes
US5866330A (en) * 1995-09-12 1999-02-02 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US5871697A (en) * 1995-10-24 1999-02-16 Curagen Corporation Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing

Also Published As

Publication number Publication date
US6225077B1 (en) 2001-05-01
CN1234076A (en) 1999-11-03
DE69711895D1 (en) 2002-05-16
GB9618544D0 (en) 1996-10-16
CA2264581C (en) 2003-11-11
WO1998010095A1 (en) 1998-03-12
JP2000517192A (en) 2000-12-26
IL128809A (en) 2002-09-12
AU4027497A (en) 1998-03-26
AU721861B2 (en) 2000-07-13
EP0927267A1 (en) 1999-07-07
IL128809A0 (en) 2000-01-31
DE69711895T2 (en) 2002-11-14
ATE215994T1 (en) 2002-04-15
NZ334426A (en) 2000-09-29
JP3863189B2 (en) 2006-12-27
CN1118581C (en) 2003-08-20
EP0927267B1 (en) 2002-04-10

Similar Documents

Publication Publication Date Title
CA2264581A1 (en) Characterising dna
EP0989193A3 (en) DNA analyzing method
CA2267524A1 (en) Identifying antisense oligonucleotide binding
DE69737450D1 (en) SEQUENCING BY LIGATION-CODED ADAPTER
AU2816797A (en) Method for determination of nucleic acid sequences and diagnostic applications thereof
WO1998048047A3 (en) Characterising dna
WO1996023079A3 (en) Method for suppressing dna fragment amplification during pcr
WO1998037238A3 (en) Gene sequencer and methods
WO1999053046A3 (en) Noncloning technique for expressing a gene of interest
AU6950791A (en) Dna fragments and dna probes and primers based thereon
Lutter DNase II digestion of the nucleosome core: Precise locations and relative exposures of sites
AU6410099A (en) (moraxella catarrhalis) protein, nucleic acid sequence and uses thereof
AU4775497A (en) Method for purifying and eventually analyzing nucleic acids from biological test samples
WO1999037764A3 (en) New members of the glypican gene family
DE69318072D1 (en) Streptococcus pneumoniae genomic DNA fragments, hybridization probes, amplification primers and methods for its detection
EP0269127A3 (en) Nucleotide sequences which are selectively expressed in pre-b cells and probes therefor
CA2220270A1 (en) Method for reducing inhibitors of nucleic acid hybridization
EP2341146A3 (en) Method for determining chromatin structure
WO1998011253A3 (en) Method and kit for diagnosing and/or quantifying by sandwich hybridisation of nucleic acid sequences on solid support
Tommerup et al. Assignment of the human genes encoding 14, 3-3 Eta (YWHAH) to 22q12, 14-3-3 zeta (YWHAZ) to 2p25. 1-p25. 2, and 14-3-3 beta (YWHAB) to 20q13. 1 by in situ hybridization
WO1998024896A3 (en) Muteins of obese protein
WO2000020629A3 (en) Quantitation of rna
Shankar et al. Further evidence that the human MUC2 gene transcripts in the intestine and trachea are identical.
EP0950713A3 (en) Recombinant adenoviral vector for gene transfer
Domenici et al. The distribution of HUMTH01 polymorphism in Northern and Central Italy

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed