CA2214800A1 - Flow electroporation chamber and method - Google Patents

Flow electroporation chamber and method

Info

Publication number
CA2214800A1
CA2214800A1 CA002214800A CA2214800A CA2214800A1 CA 2214800 A1 CA2214800 A1 CA 2214800A1 CA 002214800 A CA002214800 A CA 002214800A CA 2214800 A CA2214800 A CA 2214800A CA 2214800 A1 CA2214800 A1 CA 2214800A1
Authority
CA
Canada
Prior art keywords
blood cells
blood
red blood
plasma
erythrocytes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002214800A
Other languages
French (fr)
Other versions
CA2214800C (en
Inventor
Peter M. Meserol
Rita C. Prodell
Jesse L. Acker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Maxcyte Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/402,145 external-priority patent/US5720921A/en
Application filed by Individual filed Critical Individual
Priority to CA002363548A priority Critical patent/CA2363548A1/en
Publication of CA2214800A1 publication Critical patent/CA2214800A1/en
Application granted granted Critical
Publication of CA2214800C publication Critical patent/CA2214800C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3687—Chemical treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A61M1/3623—Means for actively controlling temperature of blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3692—Washing or rinsing blood or blood constituents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • G—PHYSICS
    • G05—CONTROLLING; REGULATING
    • G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
    • G05D23/00—Control of temperature
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3616—Batch-type treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2202/00—Special media to be introduced, removed or treated
    • A61M2202/04—Liquids
    • A61M2202/0413—Blood
    • A61M2202/0429—Red blood cells; Erythrocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2205/00—General characteristics of the apparatus
    • A61M2205/33—Controlling, regulating or measuring
    • A61M2205/3306—Optical measuring means
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2205/00—General characteristics of the apparatus
    • A61M2205/33—Controlling, regulating or measuring
    • A61M2205/3379—Masses, volumes, levels of fluids in reservoirs, flow rates
    • A61M2205/3382—Upper level detectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2205/00—General characteristics of the apparatus
    • A61M2205/36—General characteristics of the apparatus related to heating or cooling
    • A61M2205/3606—General characteristics of the apparatus related to heating or cooling cooled
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2205/00—General characteristics of the apparatus
    • A61M2205/36—General characteristics of the apparatus related to heating or cooling
    • A61M2205/3673—General characteristics of the apparatus related to heating or cooling thermo-electric, e.g. Peltier effect, thermocouples, semi-conductors

Abstract

The present invention relates to a method and apparatus for the encapsulation of biologically-active substances in red blood cells, characterized by an optionally automated, continuous-flow, self-contained electroporation system (5) which allows withdrawal of blood from a patient, separation of red blood cells, and optional recombination of blood plasma and the modified red blood cells thereby producing blood with modified biological characteristics. The present invention is particularly suited for use to encapsulate allosteric effectors of hemoglobin, thereby reducing the affinity of erythrocytes for oxygen and improving the release of oxygen from erythrocytes in tissues. The blood is introduced into the system (5) at inlet (11) and mixed with an anticoagulant (27). The blood and anticoagulant mixture is passed through a filter (18) and to a blood separation and wash bowl (44). Plasma and white blood cells are admitted into the plasma reservoir (89) while the red blood cells are retained in the wash bowl (44). The separated red blood cells are pumped out and admixed at junction (67) withan IHP solution from reservoir (50). This mixture is cooled by cooling coil (68) and then pumped to electroporation chamber (72) for treatment.
CA002214800A 1995-03-10 1996-03-11 Flow electroporation chamber and method Expired - Lifetime CA2214800C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002363548A CA2363548A1 (en) 1995-03-10 1996-03-11 Flow electroporation chamber and method

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/402,145 1995-03-10
US08/402,145 US5720921A (en) 1995-03-10 1995-03-10 Flow electroporation chamber and method
US490695P 1995-10-06 1995-10-06
US60/004,906 1995-10-06
PCT/US1996/003211 WO1996028199A1 (en) 1995-03-10 1996-03-11 Flow electroporation chamber and method

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002363548A Division CA2363548A1 (en) 1995-03-10 1996-03-11 Flow electroporation chamber and method

Publications (2)

Publication Number Publication Date
CA2214800A1 true CA2214800A1 (en) 1996-09-19
CA2214800C CA2214800C (en) 2002-01-22

Family

ID=26673634

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002214800A Expired - Lifetime CA2214800C (en) 1995-03-10 1996-03-11 Flow electroporation chamber and method

Country Status (6)

Country Link
EP (1) EP0814855A4 (en)
JP (2) JP3859709B2 (en)
KR (1) KR19980702848A (en)
CN (1) CN1195997A (en)
AU (1) AU5305096A (en)
CA (1) CA2214800C (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6773669B1 (en) 1995-03-10 2004-08-10 Maxcyte, Inc. Flow electroporation chamber and method
US7029916B2 (en) 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
US7141425B2 (en) 2001-08-22 2006-11-28 Maxcyte, Inc. Apparatus and method for electroporation of biological samples
US7771984B2 (en) 2004-05-12 2010-08-10 Maxcyte, Inc. Methods and devices related to a regulated flow electroporation chamber
CN110496276A (en) * 2013-03-13 2019-11-26 努瓦拉公司 Liquid conveying system and treatment method

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5015379B2 (en) * 1999-03-17 2012-08-29 ヘモネティクス・コーポレーション Apparatus and method for processing blood
US7601268B2 (en) * 2003-05-27 2009-10-13 Haemonetics Corporation Continuous blood filtration and method of use
CN1965079B (en) * 2004-06-12 2012-12-05 英杰生命新加坡私人有限公司 Electroporator having an elongated hollow member
CN103060191B (en) * 2004-06-12 2015-03-11 英杰生命新加坡私人有限公司 Electroporator system
KR101084528B1 (en) * 2008-04-15 2011-11-18 인비트로겐 싱가포르 피티이. 엘티디. Pipette tip for electroporation device
CN101857836B (en) * 2009-11-10 2013-06-12 昆山文曲生物微系统有限公司 Flow electroporation device and system
EP2338356A1 (en) * 2009-12-23 2011-06-29 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Reactor system for electroporation
GB201012521D0 (en) * 2010-07-27 2010-09-08 Univ Strathclyde Integrated perfusion system
CN101928666B (en) * 2010-07-30 2013-04-03 北京大学 Flow type electroporation device and system
DK2970915T3 (en) 2013-03-15 2019-11-11 Maxcyte Inc Methods and compositions for generating stable transfected cells
CA2981474A1 (en) 2015-03-31 2016-10-06 Oncosec Medical Incorporated Systems and methods for improved tissue-sensing based electroporation
CN107980059B (en) 2015-04-13 2022-11-11 美克斯细胞有限公司 Methods and compositions for modifying genomic DNA
EP3998335A3 (en) * 2015-05-08 2022-07-27 Biomagnetic Solutions LLC Apparatus and method for immunomagnetic cell separation
WO2017176253A1 (en) * 2016-04-05 2017-10-12 General Electiric Comapny Activated platelet composition with tunable growth factor level
WO2018211500A1 (en) 2017-05-14 2018-11-22 Paragate Medical Ltd. Implantable fluid extraction system
CN111372650A (en) * 2017-09-30 2020-07-03 因思科瑞普特公司 Flow-through electroporation apparatus
CN108795750A (en) * 2018-05-28 2018-11-13 深圳先进技术研究院 A kind of electroporation device with refrigerating function
CN113166706A (en) 2018-08-31 2021-07-23 查尔斯·斯塔克·德雷珀实验室有限公司 High-throughput efficient cell transfection method and device
JP7393011B2 (en) * 2018-12-20 2023-12-06 国立大学法人豊橋技術科学大学 Electroporation device and method for producing foreign substance-introduced cells
US11071860B2 (en) 2019-02-06 2021-07-27 Oncosec Medical Incorporated Systems and methods for detecting fault conditions in electroporation therapy
JP7248721B2 (en) * 2021-02-17 2023-03-29 ゼネラル・エレクトリック・カンパニイ Activated platelet composition capable of modulating growth factor levels
WO2022224803A1 (en) 2021-04-22 2022-10-27 富士フイルム株式会社 Electric punching device, introduction method of bioactive material, producing method of tissue-derived biomaterial, and tissue-derived biomaterial
WO2023223931A1 (en) * 2022-05-16 2023-11-23 富士フイルム株式会社 Electroporation device and electroporation method

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6773669B1 (en) 1995-03-10 2004-08-10 Maxcyte, Inc. Flow electroporation chamber and method
US7029916B2 (en) 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
US7141425B2 (en) 2001-08-22 2006-11-28 Maxcyte, Inc. Apparatus and method for electroporation of biological samples
US7186559B2 (en) 2001-08-22 2007-03-06 Maxcyte, Inc. Apparatus and method for electroporation of biological samples
US7771984B2 (en) 2004-05-12 2010-08-10 Maxcyte, Inc. Methods and devices related to a regulated flow electroporation chamber
US9546350B2 (en) 2004-05-12 2017-01-17 Maxcyte, Inc. Methods and devices related to a regulated flow electroporation chamber
CN110496276A (en) * 2013-03-13 2019-11-26 努瓦拉公司 Liquid conveying system and treatment method
CN110496276B (en) * 2013-03-13 2022-10-14 努瓦拉公司 Fluid delivery system and method of treatment

Also Published As

Publication number Publication date
KR19980702848A (en) 1998-08-05
AU5305096A (en) 1996-10-02
JP2007007430A (en) 2007-01-18
EP0814855A1 (en) 1998-01-07
JP4171755B2 (en) 2008-10-29
JPH11502133A (en) 1999-02-23
JP3859709B2 (en) 2006-12-20
EP0814855A4 (en) 2002-07-17
CA2214800C (en) 2002-01-22
CN1195997A (en) 1998-10-14

Similar Documents

Publication Publication Date Title
CA2214800A1 (en) Flow electroporation chamber and method
EP1466968A3 (en) Method and apparatus for encapsulation of biologically-active substances
Munro et al. Ferritin: structure, biosynthesis, and role in iron metabolism.
Brzezinska-Slebodzinska et al. Antioxidant status of dairy cows supplemented prepartum with vitamin E and selenium
Abbate et al. n-3 PUFA supplementation, monocyte PCA expression and interleukin-6 production
EP0268946A3 (en) Method for separating long-chain nucleic acids
RU2006122038A (en) FILTRATION DEVICES USING TANGENTIAL FLOW AND METHODS FOR ENRICHING STEM CELLS
EP0796040A4 (en) Method and bag set for concentrating white cells
Chun et al. Tumor necrosis serum induces a serologically distinct population of NK cells.
Sallis et al. Parathyroid hormone stimulation of phosphate uptake by rat liver mitochondria
Wagemaker et al. Erythropoietin‐independent regeneration of erythroid progenitor cells following multiple injections of hydroxyurea
EP2730586B1 (en) Protein-polypeptide complex obtained from tissues of hoofed livestock embryos, process for preparing same and pharmaceutical composition
CA2159005A1 (en) Method and apparatus for encapsulation of biologically-active substances in cells
Hirayama et al. Reduced serum hydroxyl radical scavenging activity in erythropoietin therapy resistant renal anemia
DE69624908T2 (en) METHOD FOR REACHING POPULATIONS OF RARE CELLS
Kramer et al. The effect of ischemia and recirculation, hypoxia and recovery on anti-oxidant factors and β-adrenoceptor density: Is the damage in the erythrocytes a reflection of brain damage caused by complete cerebral ischemia and by hypoxia?
ES8504210A1 (en) Process for the preparation of globin from hemoglobin, and globin obtained in this process.
CA2194270A1 (en) Method and system for cultivating cells particularly macrophages
Carozzi et al. Bone marrow erythroid precursor Ca++ regulates the response to human recombinant erythropoietin (rHuEPO) in hemodialysis patients
Zingsem et al. PBSC-collection in patients using the FRESENIUS-AS 104 leucollect-protocol.
Engel et al. Circulating CD34+ cell counts as predictive parameter for the efficacy of peripheral stem cell apheresis in Ewing tumor patients
Miki et al. Tocopherol behavior and membrane constituents in erythrocytes with oxidant stress
Lohmann et al. The importance of SH-containing substances for red blood cells in acute myeloic leukemia
SU1183900A1 (en) Method of studying exchange of cholesterin in erythrocytes
Carozzi et al. Bone Marrow Erythroid Precursor Cytoplasmic Ca2+ and 1, 25-Dihydroxyvitamin D3 Regulate the Response to Human Recombinant Erythropoietin in Uremia: In Vitro and In Vivo Studies

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20160311