CA2200463A1 - Method and apparatus for automatic focusing of biomedical specimens - Google Patents

Method and apparatus for automatic focusing of biomedical specimens

Info

Publication number
CA2200463A1
CA2200463A1 CA002200463A CA2200463A CA2200463A1 CA 2200463 A1 CA2200463 A1 CA 2200463A1 CA 002200463 A CA002200463 A CA 002200463A CA 2200463 A CA2200463 A CA 2200463A CA 2200463 A1 CA2200463 A1 CA 2200463A1
Authority
CA
Canada
Prior art keywords
images
automatic focusing
coverslip
slide
focus measure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002200463A
Other languages
French (fr)
Other versions
CA2200463C (en
Inventor
Robert C. Schmidt
Keith L. Frost
Jon W. Hayenga
James A. Stephanick
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
TriPath Imaging Inc
Original Assignee
Tripath Imaging, Inc.
Robert C. Schmidt
Neopath, Inc.
Keith L. Frost
Jon W. Hayenga
James A. Stephanick
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tripath Imaging, Inc., Robert C. Schmidt, Neopath, Inc., Keith L. Frost, Jon W. Hayenga, James A. Stephanick filed Critical Tripath Imaging, Inc.
Publication of CA2200463A1 publication Critical patent/CA2200463A1/en
Application granted granted Critical
Publication of CA2200463C publication Critical patent/CA2200463C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
    • G02B7/28—Systems for automatic generation of focusing signals

Abstract

A method for automatically focusing on a slide (201) includes the steps of locating a coverslip (401-411), acquiring images from predetermined focal depths in the slide (412), and starting at an initial focal depth proximate the surface of the coverslip (202). A set of predetermined characteristics within each of the images are measured (511) to generate at least one image measurement for each of the plurality of images. A focus measure is computed (511) for each of the images, where each focus measure is a function of at least one image measurement. A best focus location is determined (512) relative to a focal depth where an acquired image has a highest focus measure.
CA002200463A 1994-09-20 1995-08-08 Method and apparatus for automatic focusing of biomedical specimens Expired - Fee Related CA2200463C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/309,405 US5647025A (en) 1994-09-20 1994-09-20 Automatic focusing of biomedical specimens apparatus
US08/309,405 1994-09-20
PCT/US1995/010133 WO1996010196A2 (en) 1994-09-20 1995-08-08 Method and apparatus for automatic focusing of biomedical specimens

Publications (2)

Publication Number Publication Date
CA2200463A1 true CA2200463A1 (en) 1996-04-04
CA2200463C CA2200463C (en) 2002-04-16

Family

ID=23198101

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002200463A Expired - Fee Related CA2200463C (en) 1994-09-20 1995-08-08 Method and apparatus for automatic focusing of biomedical specimens

Country Status (6)

Country Link
US (1) US5647025A (en)
EP (1) EP0784805A2 (en)
JP (1) JPH10506206A (en)
AU (1) AU709136B2 (en)
CA (1) CA2200463C (en)
WO (1) WO1996010196A2 (en)

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US6763140B1 (en) 2000-09-07 2004-07-13 Chipworks Method and apparatus for focusing a micro-imaging system onto a tilted or uneven sample to acquire images of the sample

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6763140B1 (en) 2000-09-07 2004-07-13 Chipworks Method and apparatus for focusing a micro-imaging system onto a tilted or uneven sample to acquire images of the sample

Also Published As

Publication number Publication date
JPH10506206A (en) 1998-06-16
AU3217195A (en) 1996-04-19
CA2200463C (en) 2002-04-16
AU709136B2 (en) 1999-08-19
US5647025A (en) 1997-07-08
WO1996010196A3 (en) 1996-07-04
EP0784805A2 (en) 1997-07-23
WO1996010196A2 (en) 1996-04-04

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