CA2182517A1 - Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis - Google Patents

Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis

Info

Publication number
CA2182517A1
CA2182517A1 CA002182517A CA2182517A CA2182517A1 CA 2182517 A1 CA2182517 A1 CA 2182517A1 CA 002182517 A CA002182517 A CA 002182517A CA 2182517 A CA2182517 A CA 2182517A CA 2182517 A1 CA2182517 A1 CA 2182517A1
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CA
Canada
Prior art keywords
ligase
polymerase
single nucleotide
primer extension
nucleotide polymorphisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002182517A
Other languages
French (fr)
Other versions
CA2182517C (en
Inventor
Theo Nikiforov
Jonathan Karn
Philip Goelet
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Beckman Coulter Inc
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Individual
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Application filed by Individual filed Critical Individual
Publication of CA2182517A1 publication Critical patent/CA2182517A1/en
Application granted granted Critical
Publication of CA2182517C publication Critical patent/CA2182517C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Abstract

A method is provided for determining the identity of a nucleotide at a preselected site in a nucleic acid molecule. The method involves the incorporation of a nucleoside triphosphate that is complementary to the nucleotide present at the preselected site onto the terminus of a primer molecule, and their subsequent ligation to a second oligonucleotide. The reaction is monitored by detecting a specific label attached to the reaction's solid phase or by detection in solution.
CA002182517A 1994-02-07 1995-02-07 Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis Expired - Fee Related CA2182517C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US19263194A 1994-02-07 1994-02-07
US08/192,631 1994-02-07
PCT/US1995/001639 WO1995021271A1 (en) 1994-02-07 1995-02-07 Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis

Publications (2)

Publication Number Publication Date
CA2182517A1 true CA2182517A1 (en) 1995-08-10
CA2182517C CA2182517C (en) 2001-08-21

Family

ID=22710442

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002182517A Expired - Fee Related CA2182517C (en) 1994-02-07 1995-02-07 Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis

Country Status (8)

Country Link
US (2) US5679524A (en)
EP (1) EP0754240B1 (en)
JP (1) JP3175110B2 (en)
AT (1) ATE247716T1 (en)
AU (1) AU694187B2 (en)
CA (1) CA2182517C (en)
DE (1) DE69531542T2 (en)
WO (1) WO1995021271A1 (en)

Families Citing this family (371)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8236493B2 (en) 1994-10-21 2012-08-07 Affymetrix, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US6974666B1 (en) * 1994-10-21 2005-12-13 Appymetric, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5728526A (en) * 1995-06-07 1998-03-17 Oncor, Inc. Method for analyzing a nucleotide sequence
EP2290364A1 (en) 1996-04-25 2011-03-02 BioArray Solutions Ltd. Light-controlled electrokinetic assembly of particles near surfaces
US6780982B2 (en) 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
EP0843019A3 (en) * 1996-11-08 2002-10-09 Kyowa Medex Co., Ltd. Method for determination of specific nucleic acid sequence, and a reagent therefor
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US20030027126A1 (en) * 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
DE69835516D1 (en) * 1997-05-16 2006-09-21 Exact Sciences Corp ELECTROPHORETIC ANALYSIS OF MOLECULES WITH IMMOBILIZED PROBES
US7348181B2 (en) 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
US7115884B1 (en) 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
US6498009B1 (en) * 1997-10-10 2002-12-24 University Of Cincinnati β-adrenergic receptor polymorphisms
US8236500B2 (en) * 1997-10-23 2012-08-07 The United States Of America, As Represented By The Department Of Veterans Affairs Promoter variants of the alpha-7 nicotinic acetylcholine receptor
EP1027458A2 (en) 1997-11-06 2000-08-16 Mosaic Technologies Multiple sequential polynucleotide displacement reactions for signal amplification and processing
US6251660B1 (en) 1997-11-25 2001-06-26 Mosaic Technologies, Inc. Devices and methods for detecting target molecules in biological samples
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
EP1086248A1 (en) 1998-06-18 2001-03-28 Mosaic Technologies, Inc. Denaturing gradient affinity electrophoresis and methods of use thereof
JP2002518026A (en) * 1998-06-19 2002-06-25 エムティー テクノロジー， インコーポレイテッド Detection of non-viral organisms using SRPRNA
WO1999067414A1 (en) * 1998-06-24 1999-12-29 Glaxo Group Limited Nucleotide detection method
EP2360271A1 (en) 1998-06-24 2011-08-24 Illumina, Inc. Decoding of array sensors with microspheres
US6245507B1 (en) 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
US6180408B1 (en) 1998-08-21 2001-01-30 Washington University Fluorescence polarization in nucleic acid analysis
US6461812B2 (en) * 1998-09-09 2002-10-08 Agilent Technologies, Inc. Method and multiple reservoir apparatus for fabrication of biomolecular arrays
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (en) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Pre-selection and isolation of single nucleotide polymorphisms
AU6412799A (en) 1998-10-05 2000-04-26 Mosaic Technologies Reverse displacement assay for detection of nucleic acid sequences
AU772281B2 (en) 1998-12-14 2004-04-22 Li-Cor Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
WO2000047771A2 (en) 1999-02-12 2000-08-17 Genset Biallelic markers derived from genomic regions carrying genes involved in arachidonic acid metabolism
CA2369083A1 (en) * 1999-02-26 2000-08-31 Mosaic Technologies Biochemical purification devices with immobilized capture probes and their uses
US6403309B1 (en) 1999-03-19 2002-06-11 Valigen (Us), Inc. Methods for detection of nucleic acid polymorphisms using peptide-labeled oligonucleotides and antibody arrays
US6582906B1 (en) 1999-04-05 2003-06-24 Affymetrix, Inc. Proportional amplification of nucleic acids
DE60038109T2 (en) * 1999-04-09 2008-11-27 Keygene N.V. Method for analyzing AFLP reaction mixtures using primer extension techniques
AU4476900A (en) 1999-04-20 2000-11-02 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20030207295A1 (en) * 1999-04-20 2003-11-06 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6620584B1 (en) 1999-05-20 2003-09-16 Illumina Combinatorial decoding of random nucleic acid arrays
US6544732B1 (en) * 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8080380B2 (en) 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US20020119448A1 (en) * 1999-06-23 2002-08-29 Joseph A. Sorge Methods of enriching for and identifying polymorphisms
US6692915B1 (en) 1999-07-22 2004-02-17 Girish N. Nallur Sequencing a polynucleotide on a generic chip
US6864050B2 (en) 1999-07-30 2005-03-08 Affymetrix, Inc. Single-phase amplification of nucleic acids
US7604996B1 (en) 1999-08-18 2009-10-20 Illumina, Inc. Compositions and methods for preparing oligonucleotide solutions
US6528066B1 (en) * 1999-09-14 2003-03-04 University Of Iowa Research Foundation Variant varicella-zoster viruses and methods of use
JP2003510063A (en) * 1999-09-30 2003-03-18 ニュー・イングランド・バイオラブズ・インコーポレイティッド Uptake of modified nucleotides by archaeal DNA polymerase and methods related thereto
US6448010B1 (en) * 1999-10-06 2002-09-10 Amersham Pharmacia Biotech, Inc. Method for detecting mutations using arrayed primer extension
US6376177B1 (en) 1999-10-06 2002-04-23 Virtual Pro, Inc. Apparatus and method for the analysis of nucleic acids hybridization on high density NA chips
US20030190644A1 (en) 1999-10-13 2003-10-09 Andreas Braun Methods for generating databases and databases for identifying polymorphic genetic markers
DE19951189C2 (en) * 1999-10-15 2003-11-06 Epigenomics Ag Method to distinguish 5-position methylation changes from cytosine bases and cytosine to thymine mutations and to detect single nucleotide polymorphisms (SNPs) or point mutations in genomic DNA
US20010031467A1 (en) * 1999-12-10 2001-10-18 Johannes Dapprich Method for selectively isolating a nucleic acid
US6762018B1 (en) * 1999-12-23 2004-07-13 Tetragen Sa Analysis of nucleotide polymorphisms at a site
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US20020006617A1 (en) 2000-02-07 2002-01-17 Jian-Bing Fan Nucleic acid detection methods using universal priming
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US6913884B2 (en) * 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
WO2001057268A2 (en) 2000-02-07 2001-08-09 Illumina, Inc. Nucleic acid detection methods using universal priming
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7361488B2 (en) 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
CA2399908A1 (en) * 2000-02-10 2001-08-16 Todd Dickinson Array of individual arrays as substrate for bead-based simultaneous processing of samples and manufacturing method therefor
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
EP1967595A3 (en) * 2000-02-16 2008-12-03 Illumina, Inc. Parallel genotyping of multiple patient samples
DE10010281B4 (en) * 2000-02-25 2005-03-10 Epigenomics Ag Ligase / polymerase method for the detection of cytosine methylation in DNA samples
DE10010282B4 (en) 2000-02-25 2006-11-16 Epigenomics Ag Method for the detection of cytosine methylation in DNA samples
US6872552B2 (en) 2000-02-29 2005-03-29 Burt D. Ensley Method of reconstituting nucleic acid molecules
US7422850B2 (en) * 2000-05-19 2008-09-09 Eragen Biosciences, Inc. Materials and methods for detection of nucleic acids
DE10029914A1 (en) * 2000-06-19 2002-01-03 Epigenomics Ag Method for the highly parallel analysis of polymorphisms
ES2259666T3 (en) 2000-06-21 2006-10-16 Bioarray Solutions Ltd MOLECULAR ANALYSIS OF MULTIPLE ANALYTICS USING SERIES OF RANDOM PARTICLES WITH APPLICATION SPECIFICITY.
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
JP4310599B2 (en) * 2000-07-05 2009-08-12 東洋紡績株式会社 Method for detecting nucleotide polymorphisms
EP1366185B9 (en) * 2000-07-11 2007-10-17 Kirk Hogan Methods and compositions for perioperative genomic profiling
WO2002016647A1 (en) * 2000-08-24 2002-02-28 Aviva Biosciences Corporation Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
US6900013B1 (en) 2000-08-25 2005-05-31 Aviva Biosciences Corporation Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
GB0021286D0 (en) * 2000-08-30 2000-10-18 Gemini Genomics Ab Identification of drug metabolic capacity
KR20020065471A (en) * 2000-08-30 2002-08-13 산코 준야쿠 가부시키가이샤 Gene detecting method
US7465540B2 (en) 2000-09-21 2008-12-16 Luminex Corporation Multiple reporter read-out for bioassays
JP4499987B2 (en) 2000-10-14 2010-07-14 エラジェン バイオサイエンシズ インコーポレイテッド Solid support assay systems and methods using non-standard bases
EP1366192B8 (en) * 2000-10-24 2008-10-29 The Board of Trustees of the Leland Stanford Junior University Direct multiplex characterization of genomic dna
US20040018491A1 (en) * 2000-10-26 2004-01-29 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US7060442B2 (en) * 2000-10-30 2006-06-13 Regents Of The University Of Michigan Modulators on Nod2 signaling
AU2002228974A1 (en) 2000-12-13 2002-06-24 Nugen Technologies, Inc Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US20040266021A9 (en) * 2001-01-26 2004-12-30 Andras Guttman Multicapillary fraction collection system and method
MXPA02012739A (en) 2001-03-09 2004-04-20 Nugen Technologies Inc Methods and compositions for amplification of rna sequences.
US20030092157A1 (en) * 2001-03-16 2003-05-15 Hayden Michael R. Compositions, screening systems and methods for modulating HDL cholesterol and triglyceride levels
WO2004059289A2 (en) * 2001-05-22 2004-07-15 Epicentre Technologies Target-dependent transcription using deletion mutants of n4 rna polymerase
AU2002305019A1 (en) * 2001-05-25 2002-12-09 University Of British Columbia Diagnostic methods for cardiovascular disease, low hdl-cholesterol levels, and high triglyceride levels
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
US20030170695A1 (en) * 2001-06-29 2003-09-11 Liang Shi Enzymatic ligation-based identification of nucleotide sequences
US20030082584A1 (en) * 2001-06-29 2003-05-01 Liang Shi Enzymatic ligation-based identification of transcript expression
AU2002327236A1 (en) 2001-07-12 2003-01-29 Illumina, Inc. Multiplex nucleic acid reactions
WO2003062783A2 (en) * 2001-07-20 2003-07-31 North Carolina State University Light addressable electrochemical detection of duplex structures
US7153656B2 (en) * 2001-09-11 2006-12-26 Los Alamos National Security, Llc Nucleic acid sequence detection using multiplexed oligonucleotide PCR
US7108975B2 (en) * 2001-09-21 2006-09-19 Regents Of The University Of Michigan Atlastin
US7582425B2 (en) * 2001-09-21 2009-09-01 The Regents Of The University Of Michigan Atlastin
US20030207327A1 (en) * 2001-09-27 2003-11-06 Kmiec Eric B. Coisogenic eukaryotic cell collections
CA2741049C (en) * 2001-10-15 2019-02-05 Bioarray Solutions, Ltd. Multiplexed analysis of polymorphic loci by probe elongation-mediated detection
US20080138800A1 (en) * 2001-10-15 2008-06-12 Alice Xiang Li Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection
US7244562B2 (en) * 2001-11-01 2007-07-17 Gene Check, Inc. RecA assisted detection of mutations, single nucleotide polymorphisms and specific sequences
AU2002365157A1 (en) * 2001-11-08 2003-07-15 The Johns Hopkins University Methods and systems of nucleic acid sequencing
PT1458888E (en) * 2001-12-10 2011-06-01 Novartis Ag Methods of treating psychosis and schizophrenia based on polymorphisms in the cntf gene
US20030148284A1 (en) * 2001-12-17 2003-08-07 Vision Todd J. Solid phase detection of nucleic acid molecules
US7255986B2 (en) * 2002-01-31 2007-08-14 The Board Of Trustees Operating Michigan State University Compositions for the diagnosis and treatment of epizootic catarrhal enteritis in ferrets
WO2003069333A1 (en) 2002-02-14 2003-08-21 Illumina, Inc. Automated information processing in randomly ordered arrays
JP3818265B2 (en) * 2002-03-04 2006-09-06 株式会社豊田中央研究所 Light immobilization method for minute object, carrier for immobilizing minute object, and observation method for minute object
JP4274948B2 (en) * 2002-03-13 2009-06-10 シンジェンタ・パティシペーションズ・アクチェンゲゼルシャフト Nucleic acid detection method
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
US20030220844A1 (en) * 2002-05-24 2003-11-27 Marnellos Georgios E. Method and system for purchasing genetic data
WO2004013606A2 (en) * 2002-08-02 2004-02-12 Stratatech Corporation Species specific dna detection
AU2002951411A0 (en) * 2002-09-16 2002-09-26 The University Of Sydney Genotype screen
US20050003369A1 (en) * 2002-10-10 2005-01-06 Affymetrix, Inc. Method for depleting specific nucleic acids from a mixture
US6863731B2 (en) * 2002-10-18 2005-03-08 Controls Corporation Of America System for deposition of inert barrier coating to increase corrosion resistance
US7803579B2 (en) 2002-10-29 2010-09-28 Riken Process for amplifying nucleic acid
US7526114B2 (en) 2002-11-15 2009-04-28 Bioarray Solutions Ltd. Analysis, secure access to, and transmission of array images
EP2112229A3 (en) 2002-11-25 2009-12-02 Sequenom, Inc. Methods for identifying risk of breast cancer and treatments thereof
AU2003302253B2 (en) * 2002-12-12 2007-06-28 Nanosphere, Inc. Direct SNP detection with unamplified DNA
US8206904B2 (en) * 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
ATE525469T1 (en) 2002-12-18 2011-10-15 Third Wave Tech Inc DETECTION OF SMALL NUCLEIC ACIDS
US20040126765A1 (en) * 2002-12-27 2004-07-01 Adams Craig W. Method and compositions for sequencing nucleic acid molecules
US7297780B2 (en) 2003-01-06 2007-11-20 Third Wave Technologies, Inc. Reactive functional groups for preparation of modified nucleic acid
US20040180369A1 (en) * 2003-01-16 2004-09-16 North Carolina State University Photothermal detection of nucleic acid hybridization
DE602004018270D1 (en) * 2003-01-17 2009-01-22 Eragen Biosciences Inc NUCLEIC ACID AMPLIFICATION WITH NON-STANDARD BASES
WO2004065000A1 (en) 2003-01-21 2004-08-05 Illumina Inc. Chemical reaction monitor
AU2003900368A0 (en) * 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
JP4351210B2 (en) * 2003-02-14 2009-10-28 エーザイ・アール・アンド・ディー・マネジメント株式会社 Signal amplification method for detection of expressed genes
WO2004074480A1 (en) 2003-02-21 2004-09-02 Eisai Co., Ltd. Signal amplification method for detecting mutant gene
GB0304371D0 (en) 2003-02-26 2003-04-02 Solexa Ltd DNA Sequence analysis
US7820808B2 (en) 2003-04-01 2010-10-26 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
EP1613778B1 (en) 2003-04-14 2014-11-26 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
ATE476523T1 (en) 2003-06-17 2010-08-15 Human Genetic Signatures Pty METHOD FOR GENOME AMPLIFICATION
EP1636337A4 (en) 2003-06-20 2007-07-04 Illumina Inc Methods and compositions for whole genome amplification and genotyping
US20040259100A1 (en) * 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20050181394A1 (en) * 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
JP4714148B2 (en) * 2003-09-04 2011-06-29 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド Nucleic acid detection assay
WO2005025409A2 (en) * 2003-09-12 2005-03-24 University Of Cincinnati Methods for risk assessment, survival prediction and treatment of heart failure and other conditions based on adrenergic receptor polymorphisms
WO2005029705A2 (en) 2003-09-18 2005-03-31 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
ES2375962T3 (en) 2003-09-22 2012-03-07 Bioarray Solutions Ltd IMMOBILIZED SURFACE POLYELECTROLYTE WITH MULTIPLE FUNCTIONAL GROUPS ABLE TO JOIN COVALENTLY TO BIOMOLECULES.
CA2899287A1 (en) 2003-10-28 2005-05-12 Bioarray Solutions Ltd. Optimization of gene expression analysis using immobilized capture probes
AU2004287069B2 (en) 2003-10-29 2009-07-16 Bioarray Solutions, Ltd. Multiplexed nucleic acid analysis by fragmentation of double-stranded DNA
WO2005042785A1 (en) * 2003-10-30 2005-05-12 North Carolina State University Electrochemical detection of nucleic acid hybridization
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7972783B2 (en) * 2003-11-24 2011-07-05 Branhaven LLC Method and markers for determining the genotype of horned/polled cattle
GB0327587D0 (en) * 2003-11-27 2003-12-31 Genomica Sau Method for detecting nucleic acid sequence variations
US7314714B2 (en) * 2003-12-19 2008-01-01 Affymetrix, Inc. Method of oligonucleotide synthesis
WO2005064020A1 (en) * 2003-12-23 2005-07-14 Autogenomics, Inc. Multiplexed nucleic acid analysis with high specificity
US20050136414A1 (en) * 2003-12-23 2005-06-23 Kevin Gunderson Methods and compositions for making locus-specific arrays
EP2415878A1 (en) 2003-12-25 2012-02-08 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
US7432057B2 (en) * 2004-01-30 2008-10-07 Michigan State University Genetic test for PSE-susceptible turkeys
WO2005081776A2 (en) * 2004-01-30 2005-09-09 Eragen Biosciences, Inc. Materials and methods for the detection of sars
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
EP1574583A1 (en) * 2004-03-10 2005-09-14 Roche Diagnostics GmbH Methods for isolation of bacteria from biological samples
US7462451B2 (en) * 2004-04-26 2008-12-09 Third Wave Technologies, Inc. Compositions for modifying nucleic acids
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
WO2006022628A1 (en) * 2004-07-22 2006-03-02 Sequenom, Inc. Methods for identifying risk of type ii diabetes and treatments thereof
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
JP5249581B2 (en) * 2004-08-09 2013-07-31 ジェネレイション バイオテック リミテッド ライアビリティ カンパニー Nucleic acid isolation and amplification method
JP4980219B2 (en) * 2004-09-10 2012-07-18 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド Amplification blocker comprising intercalating nucleic acid (INA) containing intercalated pseudonucleotide (IPN)
US7678824B2 (en) 2004-09-14 2010-03-16 The Regents Of The University Of Colorado, A Body Corporate Methods for treatment with bucindolol based on genetic targeting
EP1666150B1 (en) 2004-11-20 2015-01-07 Roche Diagnostics GmbH Nucleic acid preparation
WO2006058393A1 (en) * 2004-12-03 2006-06-08 Human Genetic Signatures Pty Ltd Methods for simplifying microbial nucleic acids by chemical modification of cytosines
EP1828414A4 (en) * 2004-12-23 2008-12-10 Human Genetic Signatures Pty Detection of human papilloma virus
US20090124993A1 (en) 2005-02-17 2009-05-14 Burkly Linda C Treating neurological disorders
US7498136B2 (en) * 2005-03-18 2009-03-03 Eragen Biosciences, Inc. Methods for detecting multiple species and subspecies of Neisseria
ES2440953T3 (en) 2005-03-31 2014-01-31 The General Hospital Corporation Modulation of HGF / HGFR activity to treat lymphedema
AU2006235364A1 (en) 2005-04-08 2006-10-19 Marina Biotech, Inc. RNAi therapeutic for respiratory virus infection
US20070213293A1 (en) * 2005-04-08 2007-09-13 Nastech Pharmaceutical Company Inc. Rnai therapeutic for respiratory virus infection
EP1880024A4 (en) * 2005-05-12 2009-12-09 Third Wave Tech Inc Polymorphic ghsr nucleic acids and uses thereof
AU2006248189A1 (en) 2005-05-20 2006-11-23 Synergenz Bioscience Limited Methods of analysis of polymorphisms and uses thereof
EP1883707B1 (en) * 2005-05-26 2014-04-09 Human Genetic Signatures PTY Ltd Isothermal strand displacement amplification using primers containing a non-regular base
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
CA3027954C (en) 2005-06-07 2021-11-02 Luminex Corporation Methods for detection and typing of nucleic acids
WO2007002567A2 (en) * 2005-06-23 2007-01-04 Nanosphere, Inc. Selective isolation and concentration of nucleic acids from complex samples
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
EP1943353B1 (en) * 2005-09-14 2011-11-02 Human Genetic Signatures PTY Ltd Assay for a health state
CA2626604A1 (en) 2005-10-21 2007-04-26 Genenews Inc. Method and apparatus for correlating levels of biomarker products with disease
US7981606B2 (en) * 2005-12-21 2011-07-19 Roche Molecular Systems, Inc. Control for nucleic acid testing
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20070264694A1 (en) * 2006-04-07 2007-11-15 Eragen Biosciences, Inc. Use of non-standard bases and proximity effects for gene assembly and conversion of non-standard bases to standard bases during dna synthesis
US20070256148A1 (en) * 2006-04-26 2007-11-01 Katz David A DEP2 and its uses in major depressive disorder and other related disorders
US8933209B2 (en) 2006-04-26 2015-01-13 Abbvie Inc. Dep2 and its uses in major depressive disorder and other related disorders
US7674924B2 (en) * 2006-05-22 2010-03-09 Third Wave Technologies, Inc. Compositions, probes, and conjugates and uses thereof
AU2007268369A1 (en) * 2006-05-30 2007-12-06 Synergenz Bioscience Limited Methods and compositions for assessment of pulmonary function and disorders
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
EP3260556B1 (en) 2006-05-31 2019-07-31 Sequenom, Inc. Methods for the extraction of nucleic acid from a sample
CN101541975B (en) 2006-06-01 2013-04-03 第三次浪潮技术公司 Detection of nucleic acids
EP2035439A4 (en) 2006-06-05 2010-01-13 Cancer Care Ontario Assessment of risk for colorectal cancer
US7759062B2 (en) * 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
WO2008048120A2 (en) * 2006-10-17 2008-04-24 Synergenz Bioscience Limited Methods and compositions for assessment of pulmonary function and disorders
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
CA2673092A1 (en) * 2006-12-19 2008-06-26 Synergenz Bioscience Limited Methods and compositions for the assessment of cardiovascular function and disorders
WO2008098142A2 (en) 2007-02-08 2008-08-14 Sequenom, Inc. Nucleic acid-based tests for rhd typing, gender determination and nucleic acid quantification
CA2679954A1 (en) 2007-03-05 2008-09-12 Cancer Care Ontario Assessment of risk for colorectal cancer
EP2126120A4 (en) * 2007-03-16 2011-03-30 Human Genetic Signatures Pty Assay for gene expression
WO2008118988A1 (en) 2007-03-26 2008-10-02 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
AU2008260029B2 (en) 2007-05-31 2015-02-12 Yale University A genetic lesion associated with cancer
NZ556506A (en) * 2007-07-13 2010-03-26 Ovita Ltd Ovine identification method
EP2195452B1 (en) 2007-08-29 2012-03-14 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
US7811810B2 (en) 2007-10-25 2010-10-12 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US7767441B2 (en) * 2007-10-25 2010-08-03 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
JP4400668B2 (en) * 2007-11-01 2010-01-20 株式会社豊田中央研究所 Method for producing solid-phase body on which minute object is immobilized and use thereof
JP2009178159A (en) * 2007-11-05 2009-08-13 Hitachi Plant Technologies Ltd Method of nucleic acid sequence detection and nucleic acid sequence detection substrate
MX2010005578A (en) 2007-11-20 2010-06-02 Du Pont Plants with altered root architecture, related constructs and methods involving genes encoding leucine rich repeat kinase (llrk) polypeptides and homologs thereof.
AU2008329549B2 (en) * 2007-11-27 2013-08-22 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
CA2709632A1 (en) * 2007-12-20 2009-07-02 Human Genetic Signatures Pty Ltd Elimination of contaminants associated with nucleic acid amplification
WO2009128730A1 (en) 2008-01-25 2009-10-22 Patrick Gladding Methods and compositions for the assessment of drug response
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
WO2009114543A2 (en) 2008-03-11 2009-09-17 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
EP2274448B1 (en) * 2008-03-15 2013-09-18 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
CA2718137A1 (en) 2008-03-26 2009-10-01 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
EP2113574A1 (en) 2008-04-28 2009-11-04 Biotype AG Substances and methods for a DNA based profiling assay
JP2009268665A (en) * 2008-05-07 2009-11-19 Canon Inc Inhalation device
US8208909B2 (en) 2008-06-02 2012-06-26 West Corporation System, apparatus and method for availing a mobile call of address information
EP2149612A1 (en) 2008-07-29 2010-02-03 Merck Serono SA Genetic markers of response to efalizumab
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
CA2742074A1 (en) * 2008-10-29 2010-08-26 Janssen Pharmaceutica Nv Methods of treating psychosis and schizophrenia based on polymorphisms in the erbb4 gene
WO2010068738A1 (en) 2008-12-10 2010-06-17 Dana-Farber Cancer Institute, Inc. Mek mutations conferring resistance to mek inhibitors
WO2010068702A2 (en) * 2008-12-10 2010-06-17 Illumina, Inc. Methods and compositions for hybridizing nucleic acids
WO2010077832A1 (en) 2008-12-15 2010-07-08 Wisconsin Alumni Research Foundation Methods and compositions for testing and breeding cattle for improved fertility and embryonic survival
AU2010221721A1 (en) 2009-02-06 2011-08-18 Yale University A SNP marker of breast and ovarian cancer risk
JP2012517238A (en) 2009-02-11 2012-08-02 カリス エムピーアイ インコーポレイテッド Molecular profiling of tumors
WO2010115100A1 (en) 2009-04-03 2010-10-07 L&C Diagment, Inc. Multiplex nucleic acid detection methods and systems
WO2010115016A2 (en) 2009-04-03 2010-10-07 Sequenom, Inc. Nucleic acid preparation compositions and methods
US8871494B2 (en) 2009-04-24 2014-10-28 Wisconsin Alumni Research Foundation Over-production of secondary metabolites by over-expression of the VEA gene
US20110091931A1 (en) * 2009-04-24 2011-04-21 Colby Pharmaceutical Company Methods and Kits for Determining Oxygen Free Radical (OFR) Levels in Animal and Human Tissues as a Prognostic Marker for Cancer and Other Pathophysiologies
SI2430452T1 (en) 2009-05-14 2014-11-28 Arizona Board Of Regents On Behalf Of The University Of Arizona Carcinoma diagnosis and treatments, based on odc1 genotype
WO2010151841A2 (en) 2009-06-25 2010-12-29 Yale University Single nucleotide polymorphisms in brca1 and cancer risk
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
WO2011032088A1 (en) 2009-09-11 2011-03-17 Arca Biopharma, Inc. Polymorphisms in the pde3a gene
JP5938348B2 (en) * 2009-10-23 2016-06-22 ルミネックス コーポレーション Amplification primers containing non-standard bases for increased reaction specificity
EP2494077A4 (en) 2009-10-27 2013-08-21 Caris Mpi Inc Molecular profiling for personalized medicine
WO2011059686A2 (en) 2009-10-29 2011-05-19 Wisconsin Alumni Research Foundation Detection of b-cell activating factor as a biomaker for antibody mediated rejection in transplant recipients
DK2516680T3 (en) 2009-12-22 2016-05-02 Sequenom Inc Method and kits to identify aneuploidy
ES2587191T3 (en) 2009-12-23 2016-10-21 Arca Biopharma, Inc. Methods and compositions for cardiovascular diseases and conditions
US8420801B2 (en) * 2010-01-08 2013-04-16 Roche Molecular Systems, Inc. Recovery of nucleic acids from magnetic glass particles
US20110191910A1 (en) 2010-02-02 2011-08-04 E. I. Du Pont De Nemours And Company Plants with altered root architecture, related constructs and methods involving genes encoding lectin protein kinase (lpk) polypeptides and homologs thereof
EP3028699B1 (en) 2010-02-25 2018-03-21 Dana-Farber Cancer Institute, Inc. Braf mutations conferring resistance to braf inhibitors
US8536323B2 (en) 2010-04-21 2013-09-17 Pierce Biotechnology, Inc. Modified nucleotides
US9206216B2 (en) 2010-04-21 2015-12-08 Pierce Biotechnology, Inc. Modified nucleotides methods and kits
EP2568978B1 (en) 2010-05-14 2019-04-24 Arizona Board of Regents on Behalf of University of Arizona Cancer prevention and treatment based on dietary polyamine content
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
WO2011156588A1 (en) 2010-06-09 2011-12-15 Dana-Farber Cancer Institute, Inc. A mek 1 mutation conferring resistance to raf and mek inhibitors
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2013082164A1 (en) 2011-11-28 2013-06-06 Life Technologies Corporation Enhanced ligation reactions
WO2012106546A2 (en) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
US20140024590A1 (en) 2011-02-18 2014-01-23 Yale University KRAS-Variant And Endometriosis
AU2012203968A1 (en) 2011-03-21 2012-10-11 Yale University The KRAS variant and tumor biology
WO2012149339A2 (en) 2011-04-29 2012-11-01 Sequenom, Inc. Quantification of a minority nucleic acid species
MY167564A (en) 2011-09-07 2018-09-14 Human Genetic Signatures Pty Ltd Molecular detection assay
WO2013045505A1 (en) 2011-09-28 2013-04-04 Novartis Ag Biomarkers for raas combination therapy
US9605313B2 (en) 2012-03-02 2017-03-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CN104379563B (en) 2012-04-10 2018-12-21 加利福尼亚大学董事会 Composition and method for treating cancer
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
WO2014011928A1 (en) 2012-07-13 2014-01-16 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
CA2881024C (en) 2012-08-06 2021-06-15 Christoph Hubertus LADEL Genetic markers for predicting responsiveness to fgf-18 compound
US9181583B2 (en) 2012-10-23 2015-11-10 Illumina, Inc. HLA typing using selective amplification and sequencing
WO2014066864A2 (en) 2012-10-26 2014-05-01 Memorial Sloan-Kettering Cancer Center Androgen receptor variants and methods for making and using
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9896728B2 (en) 2013-01-29 2018-02-20 Arcticrx Ltd. Method for determining a therapeutic approach for the treatment of age-related macular degeneration (AMD)
DK3553175T3 (en) 2013-03-13 2021-08-23 Illumina Inc PROCEDURE FOR MAKING A NUCLEIC ACID SEQUENCE LIBRARY
EP3597774A1 (en) 2013-03-13 2020-01-22 Sequenom, Inc. Primers for dna methylation analysis
EP2971069B1 (en) 2013-03-13 2018-10-17 Illumina, Inc. Methods and systems for aligning repetitive dna elements
UY35464A (en) 2013-03-15 2014-10-31 Araxes Pharma Llc KRAS G12C COVALENT INHIBITORS.
US9670545B2 (en) 2013-06-11 2017-06-06 Coutagen Life Sciences, Inc. Methods and kits for treating and classifying individuals at risk of or suffering from TRAP1 change-of-function
TWI659021B (en) 2013-10-10 2019-05-11 亞瑞克西斯製藥公司 Inhibitors of kras g12c
AU2014364180B2 (en) 2013-12-09 2021-03-04 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
ES2890078T3 (en) 2013-12-20 2022-01-17 Illumina Inc Conservation of genomic connectivity information in fragmented genomic DNA samples
BR112016016546B1 (en) 2014-01-16 2022-09-06 Illumina, Inc AMPLICON PREPARATION METHOD AND METHODS FOR DETERMINING THE PRESENCE OF A CANCER-ASSOCIATED GENE AND A TRUE NUCLEIC ACID SEQUENCE VARIANT
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
EP3117011B1 (en) 2014-03-13 2020-05-06 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
GB201410420D0 (en) 2014-06-11 2014-07-23 Illumina Cambridge Ltd Methods for estimating cluster numbers
SG11201610357UA (en) 2014-06-13 2017-01-27 Illumina Cambridge Ltd Methods and compositions for preparing sequencing libraries
EP3161152B1 (en) 2014-06-30 2018-12-26 Illumina, Inc. Methods and compositions using one-sided transposition
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
US20160085910A1 (en) 2014-09-18 2016-03-24 Illumina, Inc. Methods and systems for analyzing nucleic acid sequencing data
JO3556B1 (en) 2014-09-18 2020-07-05 Araxes Pharma Llc Combination therapies for treatment of cancer
WO2016049568A1 (en) 2014-09-25 2016-03-31 Araxes Pharma Llc Methods and compositions for inhibition of ras
WO2016049524A1 (en) 2014-09-25 2016-03-31 Araxes Pharma Llc Inhibitors of kras g12c mutant proteins
IL299976A (en) 2014-10-17 2023-03-01 Illumina Cambridge Ltd Contiguity preserving transposition
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
WO2016116935A1 (en) 2015-01-21 2016-07-28 Yeda Research And Development Co. Ltd. Use of rasa2 as a prognostic and therapeutic marker for melanoma
EP3783109A1 (en) 2015-03-31 2021-02-24 Illumina Cambridge Limited Surface concatamerization of templates
EP3280821B1 (en) 2015-04-07 2023-12-06 Polyskope Labs Detection of one or more pathogens
KR20180005178A (en) 2015-04-10 2018-01-15 아락세스 파마 엘엘씨 Substituted quinazoline compounds and methods for their use
WO2016168540A1 (en) 2015-04-15 2016-10-20 Araxes Pharma Llc Fused-tricyclic inhibitors of kras and methods of use thereof
CN115261468A (en) 2015-07-06 2022-11-01 伊卢米纳剑桥有限公司 Sample preparation for nucleic acid amplification
US10144724B2 (en) 2015-07-22 2018-12-04 Araxes Pharma Llc Substituted quinazoline compounds and methods of use thereof
CN107849041B (en) 2015-07-23 2021-07-23 默沙东公司 Genetic markers associated with response to CRTH2 receptor antagonists
WO2017058915A1 (en) 2015-09-28 2017-04-06 Araxes Pharma Llc Inhibitors of kras g12c mutant proteins
EP3356359B1 (en) 2015-09-28 2021-10-20 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
EP3356354A1 (en) 2015-09-28 2018-08-08 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
US10858343B2 (en) 2015-09-28 2020-12-08 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
US10647703B2 (en) 2015-09-28 2020-05-12 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
EP3356339A1 (en) 2015-09-28 2018-08-08 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
EP3356347A1 (en) 2015-09-28 2018-08-08 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
EP3364977A4 (en) 2015-10-19 2019-09-04 Araxes Pharma LLC Method for screening inhibitors of ras
EP3377481A1 (en) 2015-11-16 2018-09-26 Araxes Pharma LLC 2-substituted quinazoline compounds comprising a substituted heterocyclic group and methods of use thereof
WO2017100546A1 (en) 2015-12-09 2017-06-15 Araxes Pharma Llc Methods for preparation of quinazoline derivatives
DK3377226T3 (en) 2016-03-28 2021-04-26 Illumina Inc Micro rows in several levels
US10822312B2 (en) 2016-03-30 2020-11-03 Araxes Pharma Llc Substituted quinazoline compounds and methods of use
JP2019515035A (en) 2016-04-27 2019-06-06 ミラ ディーエックス， インコーポレイテッド Immunity-based treatment of KRAS variant cancer patients
CN109689894A (en) 2016-06-19 2019-04-26 耶路撒冷希伯来大学伊萨姆研发有限公司 Chemotherapy resistance is screened in human haploid cell
US10646488B2 (en) 2016-07-13 2020-05-12 Araxes Pharma Llc Conjugates of cereblon binding compounds and G12C mutant KRAS, HRAS or NRAS protein modulating compounds and methods of use thereof
KR102475710B1 (en) 2016-07-22 2022-12-08 오레곤 헬스 앤드 사이언스 유니버시티 Single-cell whole-genome libraries and combinatorial indexing methods for their preparation
US10280172B2 (en) 2016-09-29 2019-05-07 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
US20200016594A1 (en) 2016-09-30 2020-01-16 The Governing Council Of The University Of Toronto System for identifying and targeting individual cells within a heterogeneous population for selective extraction of cellular content
CN110312711A (en) 2016-10-07 2019-10-08 亚瑞克西斯制药公司 Heterocyclic compound and its application method as RAS inhibitor
BR112019012203A2 (en) 2016-12-14 2019-11-12 Beijing Genomics Inst At Shenzhen response-associated human genetic markers for treatments that target clostridium difficile toxin b
MX2019007030A (en) 2016-12-15 2020-01-15 Univ California Compositions and methods for treating cancer.
EP3571313B1 (en) 2017-01-20 2020-12-23 Omniome, Inc. Allele-specific capture of nucleic acids
US9932631B1 (en) 2017-09-11 2018-04-03 Omniome, Inc. Genotyping by polymerase binding
WO2018140512A1 (en) 2017-01-26 2018-08-02 Araxes Pharma Llc Fused bicyclic benzoheteroaromatic compounds and methods of use thereof
US11136308B2 (en) 2017-01-26 2021-10-05 Araxes Pharma Llc Substituted quinazoline and quinazolinone compounds and methods of use thereof
EP3573971A1 (en) 2017-01-26 2019-12-04 Araxes Pharma LLC 1-(3-(6-(3-hydroxynaphthalen-1-yl)benzofuran-2-yl)azetidin-1yl)prop-2-en-1-one derivatives and similar compounds as kras g12c modulators for treating cancer
US11358959B2 (en) 2017-01-26 2022-06-14 Araxes Pharma Llc Benzothiophene and benzothiazole compounds and methods of use thereof
JP7327802B2 (en) 2017-01-26 2023-08-16 アラクセス ファーマ エルエルシー Fused hetero-heterobicyclic compounds and methods of use thereof
EP3615671B1 (en) 2017-04-23 2021-07-21 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
AU2018260627A1 (en) 2017-04-23 2019-11-14 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
US10934584B2 (en) 2017-04-23 2021-03-02 Illumina, Inc. Compositions and methods for improving sample identification in indexed nucleic acid libraries
SG10202113146UA (en) 2017-05-25 2021-12-30 Araxes Pharma Llc Covalent inhibitors of kras
JP2020521741A (en) 2017-05-25 2020-07-27 アラクセス ファーマ エルエルシー Compounds for the treatment of cancer and methods of their use
CN110831933A (en) 2017-05-25 2020-02-21 亚瑞克西斯制药公司 Quinazoline derivatives as modulators of mutated KRAS, HRAS or NRAS
US10995104B2 (en) 2017-05-30 2021-05-04 Roche Molecular System, Inc. Catalysts for reversing formaldehyde adducts and crosslinks
DK3981884T3 (en) 2017-06-07 2023-09-04 Univ Oregon Health & Science SINGLE CELL WHOLE GENOME LIBRARIES FOR METHYLATION SEQUENCE
CN110785813A (en) 2017-07-31 2020-02-11 伊鲁米那股份有限公司 Sequencing system with multi-path biological sample aggregation
KR102307473B1 (en) 2017-08-01 2021-10-01 일루미나, 인코포레이티드 Hydrogel Beads for Nucleotide Sequencing
CA3067140C (en) 2018-02-13 2023-03-21 Illumina, Inc. Dna sequencing using hydrogel beads
US20210010073A1 (en) 2018-03-22 2021-01-14 Illumina, Inc. Preparation of nucleic acid libraries from rna and dna
US20190367909A1 (en) 2018-04-02 2019-12-05 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
EP3781706A1 (en) 2018-04-20 2021-02-24 Illumina, Inc. Methods of encapsulating single cells, the encapsulated cells and uses thereof
EP4269618A3 (en) 2018-06-04 2024-01-10 Illumina, Inc. Methods of making high-throughput single-cell transcriptome libraries
CA3113271A1 (en) 2018-10-26 2020-04-30 Illumina, Inc. Modulating polymer beads for dna processing
IL262658A (en) 2018-10-28 2020-04-30 Memorial Sloan Kettering Cancer Center Prevention of age related clonal hematopoiesis and diseases associated therewith
IL263184A (en) 2018-11-21 2020-05-31 Yarden Yosef Method of treating cancer and compositions for same
WO2020113237A1 (en) 2018-11-30 2020-06-04 Caris Mpi, Inc. Next-generation molecular profiling
FI3887540T3 (en) 2018-11-30 2023-09-14 Illumina Inc Analysis of multiple analytes using a single assay
WO2020180778A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
MX2021007774A (en) 2019-07-12 2021-08-05 Illumina Cambridge Ltd Nucleic acid library preparation using electrophoresis.
BR112021012755A2 (en) 2019-07-12 2022-04-26 Illumina Cambridge Ltd Compositions and methods for preparing nucleic acid sequencing libraries using crispr/cas9 immobilized on a solid support
EP4069865A4 (en) 2019-12-02 2023-12-20 Caris MPI, Inc. Pan-cancer platinum response predictor
CA3134746A1 (en) 2019-12-19 2021-06-24 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
BR112022019541A2 (en) 2020-03-30 2022-11-16 Illumina Inc METHODS AND COMPOSITIONS FOR PREPARING NUCLEIC ACID LIBRARIES
AU2021271637A1 (en) 2020-05-12 2022-12-08 Illumina Singapore Pte. Ltd. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
AU2021299216A1 (en) 2020-06-30 2022-12-08 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless DNA
CA3190588A1 (en) 2020-08-06 2022-02-10 Illumina, Inc. Preparation of rna and dna sequencing libraries using bead-linked transposomes
WO2022040176A1 (en) 2020-08-18 2022-02-24 Illumina, Inc. Sequence-specific targeted transposition and selection and sorting of nucleic acids
WO2022053610A1 (en) 2020-09-11 2022-03-17 Illumina Cambridge Limited Methods of enriching a target sequence from a sequencing library using hairpin adaptors
WO2022079719A1 (en) 2020-10-15 2022-04-21 Yeda Research And Development Co. Ltd. Method of treating myeloid malignancies
AU2022215582A1 (en) 2021-02-04 2023-08-24 Illumina, Inc. Long indexed-linked read generation on transposome bound beads
EP4314279A1 (en) 2021-03-29 2024-02-07 Illumina, Inc. Improved methods of library preparation
EP4314328A1 (en) 2021-03-29 2024-02-07 Illumina, Inc. Compositions and methods for assessing dna damage in a library and normalizing amplicon size bias
KR20230161955A (en) 2021-03-30 2023-11-28 일루미나, 인코포레이티드 Improved methods for isothermal complementary DNA and library preparation
BR112023019945A2 (en) 2021-03-31 2023-11-14 Illumina Cambridge Ltd METHODS FOR PREPARING SEQUENCING LIBRARIES BY DIRECTIONAL TAGGING USING TRANSPOSON-BASED TECHNOLOGY WITH UNIQUE MOLECULAR IDENTIFIERS FOR ERROR CORRECTION
WO2023196572A1 (en) 2022-04-07 2023-10-12 Illumina Singapore Pte. Ltd. Altered cytidine deaminases and methods of use
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL71064A (en) * 1983-02-28 1989-10-31 Lifecodes Corp Paternity and forensic test involving analysis of dna polymorphic genetic regions
GB8311018D0 (en) * 1983-04-22 1983-05-25 Amersham Int Plc Detecting mutations in dna
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
GB8606719D0 (en) * 1986-03-19 1986-04-23 Lister Preventive Med Genetic probes
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
GB8612087D0 (en) * 1986-05-19 1986-06-25 Ici Plc Hybridisation probes
CA1323293C (en) * 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
JP2955759B2 (en) * 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド Methods for amplifying and detecting nucleic acid sequences
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
GB2228086A (en) * 1988-11-25 1990-08-15 Ici Plc Characterisation of genomic DNA
KR920700360A (en) * 1989-03-22 1992-02-19 하리크 프리드리히 Sliding bearing
WO1990013668A1 (en) * 1989-05-05 1990-11-15 Lifecodes Corporation Method for genetic analysis of a nucleic acid sample
FR2650840B1 (en) * 1989-08-11 1991-11-29 Bertin & Cie RAPID DETECTION AND / OR IDENTIFICATION OF A SINGLE BASED ON A NUCLEIC ACID SEQUENCE, AND ITS APPLICATIONS
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
ATE137269T1 (en) * 1990-01-26 1996-05-15 Abbott Lab IMPROVED METHOD FOR AMPLIFICATION OF NUCLIC ACID TARGET SEQUENCE USABLE FOR POLYMERASE AND LIGASED CHAIN REACTION
US6004744A (en) * 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
WO1992016657A1 (en) * 1991-03-13 1992-10-01 E.I. Du Pont De Nemours And Company Method of identifying a nucleotide present at a defined position in a nucleic acid
EP0726905B1 (en) * 1993-11-03 2005-03-23 Orchid BioSciences, Inc. Single nucleotide polymorphisms and their use in genetic analysis

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US5952174A (en) 1999-09-14

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