CA2170885A1 - Process for the purification of basic fibroblast growth factor - Google Patents
Process for the purification of basic fibroblast growth factorInfo
- Publication number
- CA2170885A1 CA2170885A1 CA002170885A CA2170885A CA2170885A1 CA 2170885 A1 CA2170885 A1 CA 2170885A1 CA 002170885 A CA002170885 A CA 002170885A CA 2170885 A CA2170885 A CA 2170885A CA 2170885 A1 CA2170885 A1 CA 2170885A1
- Authority
- CA
- Canada
- Prior art keywords
- growth factor
- fibroblast growth
- basic fibroblast
- purification
- cation exchange
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/50—Fibroblast growth factors [FGF]
- C07K14/503—Fibroblast growth factors [FGF] basic FGF [bFGF]
Abstract
The invention relates to a method of purifying basic fibroblast growth factor. The method involves the use of strong cation exchange chromatography followed by hydrophobic interaction chromatography and then by weak cation exchange chromatography.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002170885A CA2170885C (en) | 1994-07-18 | 1994-07-18 | Process for the purification of basic fibroblast growth factor |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002170885A CA2170885C (en) | 1994-07-18 | 1994-07-18 | Process for the purification of basic fibroblast growth factor |
PCT/US1994/008016 WO1996002562A1 (en) | 1993-04-12 | 1994-07-18 | Process for the purification of basic fibroblast growth factor |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2170885A1 true CA2170885A1 (en) | 1996-02-01 |
CA2170885C CA2170885C (en) | 2004-05-04 |
Family
ID=22242753
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002170885A Expired - Lifetime CA2170885C (en) | 1994-07-18 | 1994-07-18 | Process for the purification of basic fibroblast growth factor |
Country Status (7)
Country | Link |
---|---|
US (1) | US5331095A (en) |
EP (1) | EP0720618B1 (en) |
JP (1) | JP3509104B2 (en) |
AT (1) | ATE240969T1 (en) |
CA (1) | CA2170885C (en) |
DE (1) | DE69432706T2 (en) |
WO (1) | WO1996002562A1 (en) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5331095A (en) * | 1993-04-12 | 1994-07-19 | Scios Nova Inc. | Process for purification of basic fibroblast growth factor |
US6008328A (en) * | 1994-10-13 | 1999-12-28 | Amgen Inc. | Method for purifying keratinocyte growth factors |
US6274712B1 (en) | 1997-12-23 | 2001-08-14 | 3-Dimensional Pharmaceuticals, Inc. | Analogs of human basic fibroblast growth factor mutated at one or more of the positions glutamute 89, aspartate 101 or leucine 137 |
EP1360192A1 (en) * | 2001-01-16 | 2003-11-12 | Kobenhavns Universitet | A method for refolding of proteins |
DE102005033250A1 (en) | 2005-07-15 | 2007-01-18 | Bioceuticals Arzneimittel Ag | Process for purifying G-CSF |
EP1973942B1 (en) | 2005-12-22 | 2011-02-09 | Genentech, Inc. | Recombinant production of heparin binding proteins |
JP5566104B2 (en) | 2006-07-14 | 2014-08-06 | ジェネンテック, インコーポレイテッド | Refolding of recombinant proteins |
IL210162A0 (en) * | 2010-12-21 | 2011-03-31 | Omrix Biopharmaceuticals | Viral inactivated platelet extract, use and preparation thereof |
EP2726496B1 (en) * | 2011-07-01 | 2018-04-04 | F.Hoffmann-La Roche Ag | Method for separation of monomeric polypeptides from aggregated polypeptides |
JP6548293B2 (en) * | 2015-09-30 | 2019-07-24 | 片山化学工業株式会社 | Method of purifying bFGF and method of producing bFGF |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4444760A (en) * | 1983-06-17 | 1984-04-24 | Merck & Co., Inc. | Purification and characterization of a protein fibroblast growth factor |
US4785079A (en) * | 1984-11-09 | 1988-11-15 | The Salk Institute For Biological Studies | Isolation of fibroblast growth factor |
US5171842A (en) * | 1988-01-25 | 1992-12-15 | American Cyanamid Company | Heparin-binding brain mitogens |
WO1989008117A1 (en) * | 1988-02-24 | 1989-09-08 | Yuen Shing | Heparin-copper bi-affinity chromatography of fibroblast growth factors |
US5120715A (en) * | 1988-12-12 | 1992-06-09 | Takeda Chemical Industries, Ltd. | Method for purifying fibroblast growth factor protein |
US5130418A (en) * | 1989-05-02 | 1992-07-14 | California Biotechnology Inc. | Method to stabilize basic fibroblast growth factor |
CA2020654A1 (en) * | 1989-07-07 | 1991-01-08 | Yohko Akiyama | Stabilized fgf composition and production thereof |
CA2020720A1 (en) * | 1989-07-13 | 1991-01-14 | Shigeko Yamazaki | Method for the purification of therapeutically active recombinant acidic fibroblast growth factor |
US5043431A (en) * | 1989-09-11 | 1991-08-27 | Codon | Method and apparatus for the production of TGF-β |
US5136025A (en) * | 1990-04-04 | 1992-08-04 | California Biotechnology Inc. | Method to purify basic fibroblast growth factor |
US5331095A (en) * | 1993-04-12 | 1994-07-19 | Scios Nova Inc. | Process for purification of basic fibroblast growth factor |
-
1993
- 1993-04-12 US US08/045,929 patent/US5331095A/en not_active Expired - Lifetime
-
1994
- 1994-07-18 DE DE69432706T patent/DE69432706T2/en not_active Expired - Lifetime
- 1994-07-18 JP JP50497696A patent/JP3509104B2/en not_active Expired - Lifetime
- 1994-07-18 EP EP94925093A patent/EP0720618B1/en not_active Expired - Lifetime
- 1994-07-18 AT AT94925093T patent/ATE240969T1/en not_active IP Right Cessation
- 1994-07-18 WO PCT/US1994/008016 patent/WO1996002562A1/en active IP Right Grant
- 1994-07-18 CA CA002170885A patent/CA2170885C/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE69432706T2 (en) | 2004-03-25 |
DE69432706D1 (en) | 2003-06-26 |
US5331095A (en) | 1994-07-19 |
WO1996002562A1 (en) | 1996-02-01 |
EP0720618A1 (en) | 1996-07-10 |
JP3509104B2 (en) | 2004-03-22 |
EP0720618B1 (en) | 2003-05-21 |
CA2170885C (en) | 2004-05-04 |
JPH08510762A (en) | 1996-11-12 |
EP0720618A4 (en) | 1997-12-03 |
ATE240969T1 (en) | 2003-06-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20140718 |