CA2165935A1 - Calibrating and testing immunoassays to minimize interferences - Google Patents

Calibrating and testing immunoassays to minimize interferences

Info

Publication number
CA2165935A1
CA2165935A1 CA002165935A CA2165935A CA2165935A1 CA 2165935 A1 CA2165935 A1 CA 2165935A1 CA 002165935 A CA002165935 A CA 002165935A CA 2165935 A CA2165935 A CA 2165935A CA 2165935 A1 CA2165935 A1 CA 2165935A1
Authority
CA
Canada
Prior art keywords
target analyte
test elements
interferent
rate
change
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002165935A
Other languages
French (fr)
Other versions
CA2165935C (en
Inventor
Merrit Nyles Jacobs
Paul Arthur Kildal-Brandt
Thomas Charles Arter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2165935A1 publication Critical patent/CA2165935A1/en
Application granted granted Critical
Publication of CA2165935C publication Critical patent/CA2165935C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds

Abstract

A method for reducing interferent bias such as hemoglobin bias in immunoassays using dried slide test elements featuring peroxidase and leuco dye as the labeling mechanism. The method features the steps of: a) calibrating an immunoassay of a target analyte by ascertaining the time at which the rate of change in density produced by a first set of the test elements for the target analyte on at least one liquid having a known amount of interferent of choice and a known amount of target analyte, crosses over or minimizes the difference compared to the rate of change in density produced by a second set of test elements substantially identical to the first set of test elements, on a liquid having the known amount of target analyte but a negligible amount of interferent of choice, and b) conducting an assay of a patient sample of unknown amount of the target analyte by reading the rate of change in density in a third set of the test elements substantially identical to the first and second sets, caused by the sample, at a time corresponding to the cross-over time ascertained in step a).
CA002165935A 1994-12-22 1995-12-21 Calibrating and testing immunoassays to minimize interferences Expired - Fee Related CA2165935C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/363,099 1994-12-22
US08/363,099 US5571682A (en) 1994-12-22 1994-12-22 Calibrating and testing immunoassays to minimize interferences

Publications (2)

Publication Number Publication Date
CA2165935A1 true CA2165935A1 (en) 1996-06-23
CA2165935C CA2165935C (en) 2009-10-20

Family

ID=23428799

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002165935A Expired - Fee Related CA2165935C (en) 1994-12-22 1995-12-21 Calibrating and testing immunoassays to minimize interferences

Country Status (7)

Country Link
US (1) US5571682A (en)
EP (1) EP0718626B1 (en)
JP (1) JP3672991B2 (en)
AT (1) ATE206519T1 (en)
AU (1) AU4056395A (en)
CA (1) CA2165935C (en)
DE (1) DE69523027T2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9066695B2 (en) 1998-04-30 2015-06-30 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US6949816B2 (en) 2003-04-21 2005-09-27 Motorola, Inc. Semiconductor component having first surface area for electrically coupling to a semiconductor chip and second surface area for electrically coupling to a substrate, and method of manufacturing same
US8688188B2 (en) 1998-04-30 2014-04-01 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8346337B2 (en) 1998-04-30 2013-01-01 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US6175752B1 (en) 1998-04-30 2001-01-16 Therasense, Inc. Analyte monitoring device and methods of use
US8480580B2 (en) 1998-04-30 2013-07-09 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8465425B2 (en) 1998-04-30 2013-06-18 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8974386B2 (en) 1998-04-30 2015-03-10 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US6560471B1 (en) 2001-01-02 2003-05-06 Therasense, Inc. Analyte monitoring device and methods of use
US7189520B2 (en) * 2002-12-12 2007-03-13 Strategic Diagnostics Inc. Compositions and methods for detecting animal byproduct in feed
JP4285012B2 (en) * 2003-01-31 2009-06-24 株式会社日立製作所 Learning situation judgment program and user situation judgment system
WO2007143225A2 (en) 2006-06-07 2007-12-13 Abbott Diabetes Care, Inc. Analyte monitoring system and method
US9023599B2 (en) * 2011-09-25 2015-05-05 Chang Gung Medical Foundation, Linkou Branch Method of detecting the presence of pneumococcal neuraminidases in Streptococcus pneumoniae-infected samples

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4022577A (en) * 1976-03-12 1977-05-10 The University Of Virginia Automated radioimmunoassay
US4184923A (en) * 1977-11-03 1980-01-22 Eastman Kodak Company Reduction of gentisic acid interference in analytical elements
JPS58140636A (en) * 1982-02-16 1983-08-20 Asahi Chem Ind Co Ltd Measuring method of electric potential of erythrocyte membrane
US4835110A (en) * 1985-12-23 1989-05-30 Beckman Instruments, Inc. Method for enhancing analysis timing in kinetic nephelometry
US5171688A (en) * 1988-08-30 1992-12-15 Cholestech Corporation Self-corrected assay device
JPH02167446A (en) * 1988-09-01 1990-06-27 Konica Corp Analysis of liquid sample
JP2646731B2 (en) * 1989-02-28 1997-08-27 株式会社島津製作所 Biochemical analysis method
EP0606950A3 (en) * 1993-01-13 1995-07-12 Eastman Kodak Co A method for reaction rate determination of analytes.

Also Published As

Publication number Publication date
CA2165935C (en) 2009-10-20
ATE206519T1 (en) 2001-10-15
EP0718626B1 (en) 2001-10-04
EP0718626A2 (en) 1996-06-26
JP3672991B2 (en) 2005-07-20
JPH08233818A (en) 1996-09-13
DE69523027T2 (en) 2002-03-14
US5571682A (en) 1996-11-05
EP0718626A3 (en) 1998-06-10
DE69523027D1 (en) 2001-11-08
AU4056395A (en) 1996-06-27

Similar Documents

Publication Publication Date Title
CA2165935A1 (en) Calibrating and testing immunoassays to minimize interferences
EP0372755B1 (en) Method of distinguishing among strips for different assays in an automated instrument
US20030235858A1 (en) Method for selectively combining multiple membranes for assembly into test strips
NO172517C (en) IMMUNO ANALYSIS FOR DETERMINATION OF ANALYTY CONCENTRATION IN A LIQUID TEST AND SET FOR IMPLEMENTATION OF THE PROCEDURE
CA2336985A1 (en) Method of glucose or galactose detection with glucose/galactose binding protein
NO953872L (en) Chromatographic analyzer with elements that can be made to resist
DK0779367T3 (en) Analyzer with stable coupling dye for photometric determination of analytes
WO2003001964A3 (en) Measurement testing of blood specimens
TR200000402T2 (en) Method and arrangement for the establishment of basic standards for use in spectroscopic analysis.
CA2427089A1 (en) Devices and methods for analyte concentration determination
WO2003043487A3 (en) Method of using a non-antibody protein to detect and measure an analyte
DE68913086T2 (en) Qualitative enzyme test for visual evaluation.
CA2130947A1 (en) Dry Elements, Test Devices, Test Kits and Methods for Chemiluminescent Detection of Analytes Using Peroxidase-Labeled Reagents
ATE97487T1 (en) ANALYTICAL TEST PROCEDURE.
WO2002014883A3 (en) Analog signal testing circuit and -method
Litman et al. An internally referenced test strip immunoassay for morphine.
CA2274776A1 (en) Method for analyzing annexin v in urine and use thereof
EP0347839A3 (en) Dry-type analytical element for immunoassay
CA2339023A1 (en) An in-vitro method of detecting and diagnosing acute coronary syndromes
DE69114247D1 (en) ANALYTICAL TEST.
WO2003019331A3 (en) Method of conducting and documenting analyte screening tests
WO1996019731A3 (en) Differential timing method for detecting multiple analytes in a test sample
JPS55146040A (en) Measuring method of concentration
EP0310940A3 (en) Analytical element for enzyme immunoassays
ATE104058T1 (en) RECEPTOR PREINCUBATION ENZYME TEST.

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20121221