CA2162336A1 - Electrophoresis in Low Conductivity Buffers - Google Patents

Electrophoresis in Low Conductivity Buffers

Info

Publication number
CA2162336A1
CA2162336A1 CA2162336A CA2162336A CA2162336A1 CA 2162336 A1 CA2162336 A1 CA 2162336A1 CA 2162336 A CA2162336 A CA 2162336A CA 2162336 A CA2162336 A CA 2162336A CA 2162336 A1 CA2162336 A1 CA 2162336A1
Authority
CA
Canada
Prior art keywords
electrophoresis
ampholytes
buffering
buffering agents
molecular weight
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2162336A
Other languages
French (fr)
Other versions
CA2162336C (en
Inventor
Stellan Hjerten
Jia-Li Liao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of CA2162336A1 publication Critical patent/CA2162336A1/en
Application granted granted Critical
Publication of CA2162336C publication Critical patent/CA2162336C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Abstract

Electrophoresis is performed in buffers which exhibit both substantial bufferingcapacity and low electrical conductivity, permitting the separations to be performed at high field strengths without loss of resolution. Four classes of buffering agents are cited as examples:
(1) buffering agents with a small number of charged groups per molecule;
(2) carrier ampholytes fractionated to a narrow isoelectric point range;
(3) low molecular weight buffering ampholytes with an isoelectric point which is close in value to one of the pK values of the ampholyte; and (4) high molecular weight buffering ampholytes in which the acidic and basic groups have the same or very close pK values.
These buffering agents are of particular interest in capillary electrophoresis.
CA002162336A 1995-01-30 1995-11-07 Electrophoresis in low conductivity buffers Expired - Fee Related CA2162336C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/380,425 US5464517A (en) 1995-01-30 1995-01-30 Electrophoresis in low conductivity buffers
US08/380,425 1995-01-30

Publications (2)

Publication Number Publication Date
CA2162336A1 true CA2162336A1 (en) 1996-07-31
CA2162336C CA2162336C (en) 1999-03-30

Family

ID=23501118

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002162336A Expired - Fee Related CA2162336C (en) 1995-01-30 1995-11-07 Electrophoresis in low conductivity buffers

Country Status (4)

Country Link
US (1) US5464517A (en)
EP (1) EP0724154A1 (en)
JP (1) JP2883565B2 (en)
CA (1) CA2162336C (en)

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US6017696A (en) 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5849486A (en) 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US6309602B1 (en) 1993-11-01 2001-10-30 Nanogen, Inc. Stacked, reconfigurable system for electrophoretic transport of charged materials
US7101661B1 (en) 1993-11-01 2006-09-05 Nanogen, Inc. Apparatus for active programmable matrix devices
US6225059B1 (en) 1993-11-01 2001-05-01 Nanogen, Inc. Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics
US7314708B1 (en) * 1998-08-04 2008-01-01 Nanogen, Inc. Method and apparatus for electronic synthesis of molecular structures
US7172864B1 (en) 1993-11-01 2007-02-06 Nanogen Methods for electronically-controlled enzymatic reactions
US7582421B2 (en) * 1993-11-01 2009-09-01 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US6331274B1 (en) 1993-11-01 2001-12-18 Nanogen, Inc. Advanced active circuits and devices for molecular biological analysis and diagnostics
US6783651B1 (en) * 1994-03-31 2004-08-31 Invitrogen Corporation System for pH-neutral stable electrophoresis gel
US6403367B1 (en) 1994-07-07 2002-06-11 Nanogen, Inc. Integrated portable biological detection system
US7857957B2 (en) 1994-07-07 2010-12-28 Gamida For Life B.V. Integrated portable biological detection system
US6379897B1 (en) * 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
US7824532B2 (en) 1995-04-26 2010-11-02 Life Technologies Corporation Apparatus and method for electrophoresis
AU6519496A (en) * 1995-07-18 1997-02-18 Waters Investments Limited Buffer/additives electrolyte combinations for electrokinetic chromatography
US20040086917A1 (en) * 1995-09-27 2004-05-06 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
DE19741715A1 (en) * 1997-09-22 1999-03-25 Hoechst Ag New pentopyranosyl nucleoside compounds
US6706161B1 (en) 1998-06-10 2004-03-16 Agilent Technologies, Inc. Methods and compositions for modifying the surface of a polymeric substrate
US6562213B1 (en) * 2000-08-30 2003-05-13 Ethrog Biotechnology Ltd. Electrophoresis apparatus for simultaneous loading of multiple samples
WO2002071024A2 (en) * 2001-03-08 2002-09-12 Ethrog Biotechnology Ltd. Apparatus and method for electrophoresis
US7601493B2 (en) * 2002-07-26 2009-10-13 Nanogen, Inc. Methods and apparatus for screening and detecting multiple genetic mutations
AU2004269196B2 (en) * 2003-09-03 2010-03-04 Shmuel Bukshpan Methods and apparatus for rapid crystallization of biomolecules
US20050161332A1 (en) * 2004-01-23 2005-07-28 Gyula Vigh Method and apparatus to improve the concentration detection sensitivity in isoelectric focusing systems
US20060049052A1 (en) * 2004-09-02 2006-03-09 The Texas A&M University System Ampholytic buffer having high buffering capacity and high conductivity in isoelectric form
US7314542B2 (en) * 2004-09-23 2008-01-01 Nanogen, Inc. Methods and materials for optimization of electronic transportation and hybridization reactions
US20080272002A1 (en) * 2007-05-03 2008-11-06 Protein Forest, Inc. System and Method for Proteomics
US20100032296A1 (en) * 2008-08-07 2010-02-11 Protein Forest, Inc. Systems and methods for quantitative analyte transfer
EP3296732A1 (en) 2009-08-24 2018-03-21 Life Technologies Corporation System for rapid high-resolution gel electrophoresis
WO2011134072A1 (en) 2010-04-27 2011-11-03 Dgel Electrosystem Inc. Electrophoresis buffer for faster migration, improved resolution and extended shelf-life
US9250208B2 (en) 2011-07-14 2016-02-02 Dgel Electrosystem Inc. Electrophoresis buffer for extending the useful electrophoresis life of an electrophoresis gel
JP6232591B2 (en) * 2016-04-27 2017-11-22 パナソニックＩｐマネジメント株式会社 Method for determining whether a capillary filled with an electrophoretic medium is properly used for electrophoresis
JP6895678B2 (en) * 2017-02-09 2021-06-30 学校法人上智学院 Supramolecular complex manufacturing method, supramolecular complex and electrolyte

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63149563A (en) * 1986-12-12 1988-06-22 Hitachi Ltd Apparatus for determining nucleic acid base sequence
EP0292837B1 (en) * 1987-05-27 1993-08-18 Abbott Laboratories Polycationic buffers and method for gel electrophoresis of nucleic acids
US4936963A (en) * 1987-05-27 1990-06-26 Abbott Laboratories Polycationic buffers and method for gel electrophoresis of nucleic acids
DE69201572T2 (en) * 1991-01-11 1995-12-21 Millipore Corp Process for performing highly effective depositions by capillary electrophoresis.

Also Published As

Publication number Publication date
EP0724154A1 (en) 1996-07-31
JP2883565B2 (en) 1999-04-19
JPH08220069A (en) 1996-08-30
CA2162336C (en) 1999-03-30
US5464517A (en) 1995-11-07

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