CA2153350A1 - A method and a device for quantifying a substrate in a sample liquid using a biosensor - Google Patents

A method and a device for quantifying a substrate in a sample liquid using a biosensor

Info

Publication number
CA2153350A1
CA2153350A1 CA002153350A CA2153350A CA2153350A1 CA 2153350 A1 CA2153350 A1 CA 2153350A1 CA 002153350 A CA002153350 A CA 002153350A CA 2153350 A CA2153350 A CA 2153350A CA 2153350 A1 CA2153350 A1 CA 2153350A1
Authority
CA
Canada
Prior art keywords
electrode
substrate
sample liquid
biosensor
counter electrode
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002153350A
Other languages
French (fr)
Other versions
CA2153350C (en
Inventor
Shin Ikeda
Toshihiko Yoshioka
Shiro Nankai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PHC Holdings Corp
Original Assignee
Matsushita Electric Industrial Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=13098814&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2153350(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Matsushita Electric Industrial Co Ltd filed Critical Matsushita Electric Industrial Co Ltd
Publication of CA2153350A1 publication Critical patent/CA2153350A1/en
Application granted granted Critical
Publication of CA2153350C publication Critical patent/CA2153350C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/817—Enzyme or microbe electrode

Abstract

A method and a device for quantifying a substrate in a sample liquid by use of a biosensor are provided. The biosensor can quantify the substrate by electrochemically measuring an amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate in the sample liquid and an oxidoreductase. The biosensor includes: an electrically insulating substrate; an electrode system formed on the substrate including a working electrode, a counter electrode, and a third electrode used for detecting a liquid junction; and a reaction layer that is formed over at least the working electrode and the counter electrode of the electrode system and includes the oxidoreductase. The method includes the steps of: applying a voltage between the counter electrode and the third electrode; supplying the sample liquid to the reaction layer; detecting an electrical change between the counter electrode and the third electrode generated by the supply of the sample liquid to the reaction layer; applying a voltage to the working electrode after the detection using at least the third electrode as a reference; and measuring a current generated between the working electrode and the counter electrode.
CA002153350A 1995-03-17 1995-07-06 A method and a device for quantifying a substrate in a sample liquid using a biosensor Expired - Lifetime CA2153350C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP5893995 1995-03-17
JP7-58939 1995-03-17

Publications (2)

Publication Number Publication Date
CA2153350A1 true CA2153350A1 (en) 1996-09-18
CA2153350C CA2153350C (en) 2001-09-04

Family

ID=13098814

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002153350A Expired - Lifetime CA2153350C (en) 1995-03-17 1995-07-06 A method and a device for quantifying a substrate in a sample liquid using a biosensor

Country Status (4)

Country Link
US (1) US5582697A (en)
EP (2) EP0732406B1 (en)
CA (1) CA2153350C (en)
DE (2) DE69533666T2 (en)

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