CA2146381A1 - Method for Quantifying Specific Compound - Google Patents

Method for Quantifying Specific Compound

Info

Publication number
CA2146381A1
CA2146381A1 CA2146381A CA2146381A CA2146381A1 CA 2146381 A1 CA2146381 A1 CA 2146381A1 CA 2146381 A CA2146381 A CA 2146381A CA 2146381 A CA2146381 A CA 2146381A CA 2146381 A1 CA2146381 A1 CA 2146381A1
Authority
CA
Canada
Prior art keywords
specific compound
quantifying
sample
short
reaction layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2146381A
Other languages
French (fr)
Other versions
CA2146381C (en
Inventor
Shin Ikeda
Toshihiko Yoshioka
Shiro Nankai
Haruhiro Tsutsumi
Hideyuki Baba
Yoshinobu Tokuno
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Panasonic Holdings Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2146381A1 publication Critical patent/CA2146381A1/en
Application granted granted Critical
Publication of CA2146381C publication Critical patent/CA2146381C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/817—Enzyme or microbe electrode

Abstract

A biosensor for quantifying a specific compound has a reaction layer containing at least an enzyme, and an electrode system having a working electrode and a counter electrode formed on an insulating base plate; the biosensor detects the specific compound contained in a sample on the basis of an electrochemical response. In quantifying, the working electrode and the counter electrode are short-circuited before the voltage is applied therebetween. The short-circuiting eliminates measuring errors that may occur due to nonuniform dissolution of the reaction layer in a sample, and achieves highly reliable quantification of a specific compound.
CA002146381A 1994-04-25 1995-04-05 Method for quantifying specific compound Expired - Fee Related CA2146381C (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JPHEI6-086579 1994-04-25
JP8657994 1994-04-25
JPHEI7-048529 1995-03-08
JP7048529A JP3061351B2 (en) 1994-04-25 1995-03-08 Method and apparatus for quantifying specific compounds

Publications (2)

Publication Number Publication Date
CA2146381A1 true CA2146381A1 (en) 1995-10-26
CA2146381C CA2146381C (en) 2000-05-09

Family

ID=26388817

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002146381A Expired - Fee Related CA2146381C (en) 1994-04-25 1995-04-05 Method for quantifying specific compound

Country Status (5)

Country Link
US (1) US5565085A (en)
EP (1) EP0679720B1 (en)
JP (1) JP3061351B2 (en)
CA (1) CA2146381C (en)
DE (1) DE69529936T2 (en)

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Also Published As

Publication number Publication date
DE69529936D1 (en) 2003-04-24
DE69529936T2 (en) 2004-01-29
US5565085A (en) 1996-10-15
CA2146381C (en) 2000-05-09
JP3061351B2 (en) 2000-07-10
EP0679720A1 (en) 1995-11-02
EP0679720B1 (en) 2003-03-19
JPH0815220A (en) 1996-01-19

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Effective date: 20130405