CA2138240A1 - Procede de preparation de polynucleotides sur support solide et appareil permettant sa mise en oeuvre - Google Patents

Procede de preparation de polynucleotides sur support solide et appareil permettant sa mise en oeuvre

Info

Publication number
CA2138240A1
CA2138240A1 CA002138240A CA2138240A CA2138240A1 CA 2138240 A1 CA2138240 A1 CA 2138240A1 CA 002138240 A CA002138240 A CA 002138240A CA 2138240 A CA2138240 A CA 2138240A CA 2138240 A1 CA2138240 A1 CA 2138240A1
Authority
CA
Canada
Prior art keywords
reactor
column
solutions
solid support
polynucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002138240A
Other languages
English (en)
Other versions
CA2138240C (fr
Inventor
Francois Chatelain
Viktor Kumarev
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Aldrich Co LLC
Original Assignee
Francois Chatelain
Viktor Kumarev
Genset
Proligo, L.L.C.
Sigma-Aldrich Co.
Sigma-Aldrich Co. Newco Inc.
Sigma-Aldrich Co. Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Francois Chatelain, Viktor Kumarev, Genset, Proligo, L.L.C., Sigma-Aldrich Co., Sigma-Aldrich Co. Newco Inc., Sigma-Aldrich Co. Llc filed Critical Francois Chatelain
Publication of CA2138240A1 publication Critical patent/CA2138240A1/fr
Application granted granted Critical
Publication of CA2138240C publication Critical patent/CA2138240C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00286—Reactor vessels with top and bottom openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00308—Reactor vessels in a multiple arrangement interchangeably mounted in racks or blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00324—Reactor vessels in a multiple arrangement the reactor vessels or wells being arranged in plates moving in parallel to each other
    • B01J2219/00328—Movement by linear translation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00389—Feeding through valves
    • B01J2219/00409—Solenoids in combination with valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Abstract

La présente invention concerne un procédé de préparation de polynucléotides sur support solide dans un réacteur en forme de colonne à travers laquelle on fait circuler les solutions de réactifs et/ou solvants, caractérisé en ce que la phase solide constituant ledit support solide est immobilisée dans ledit réacteur, et lesdites solutions migrent dans la colonne et à travers la phase solide selon une progression frontale, de sorte que les solutions successives de chaque étape d'un cycle de synthèse ne se mélangent pas ou peu. La présente invention a également pour objet un réacteur constitué par une colonne entièrement remplie par des particules de matériaux poreux constituant le support solide, et un dispositif de synthèse incluant un tel réacteur.
CA002138240A 1993-12-16 1994-12-15 Procede de preparation de polynucleotides sur support solide et appareil permettant sa mise en oeuvre Expired - Lifetime CA2138240C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9315164A FR2714061B1 (fr) 1993-12-16 1993-12-16 Procédé de préparation de polynucléotides sur support solide et appareil permettant sa mise en Óoeuvre.
FR9315164 1993-12-16

Publications (2)

Publication Number Publication Date
CA2138240A1 true CA2138240A1 (fr) 1995-06-17
CA2138240C CA2138240C (fr) 2005-04-05

Family

ID=9454019

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002138240A Expired - Lifetime CA2138240C (fr) 1993-12-16 1994-12-15 Procede de preparation de polynucleotides sur support solide et appareil permettant sa mise en oeuvre

Country Status (14)

Country Link
US (3) US5869643A (fr)
EP (1) EP0658566B1 (fr)
JP (1) JPH08239397A (fr)
KR (1) KR100366862B1 (fr)
AT (1) ATE154936T1 (fr)
AU (1) AU693487B2 (fr)
CA (1) CA2138240C (fr)
DE (1) DE69404018T2 (fr)
DK (1) DK0658566T3 (fr)
ES (1) ES2106479T3 (fr)
FR (1) FR2714061B1 (fr)
GR (1) GR3024829T3 (fr)
HK (1) HK1002330A1 (fr)
TW (1) TW349101B (fr)

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US6994827B2 (en) * 2000-06-03 2006-02-07 Symyx Technologies, Inc. Parallel semicontinuous or continuous reactors
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US20060141507A1 (en) * 2000-09-27 2006-06-29 Kronick Mel N Manufacture and use of non-standard size microarray slides
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US6761855B1 (en) 2001-11-05 2004-07-13 Biosearch Technologies, Inc. Column for solid phase processing
US20040082058A1 (en) * 2002-10-29 2004-04-29 Arthur Schleifer Array hybridization apparatus and method for making uniform sample volumes
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DE10361003B3 (de) * 2003-12-23 2005-07-28 Hte Ag The High Throughput Experimentation Company Vorrichtung und Verfahren zur Druck- und Flusskontrolle in Parallelreaktoren
US20050196761A1 (en) * 2004-03-08 2005-09-08 Thompson Allen C. Array hybridization apparatus and method
US20050214930A1 (en) * 2004-03-26 2005-09-29 Bynum Magdalena A Apparatus and method for preventing cross contamination when dissassembling an array
US20050227239A1 (en) * 2004-04-08 2005-10-13 Joyce Timothy H Microarray based affinity purification and analysis device coupled with solid state nanopore electrodes
US7560417B2 (en) * 2005-01-13 2009-07-14 Wisconsin Alumni Research Foundation Method and apparatus for parallel synthesis of chain molecules such as DNA
US7396676B2 (en) 2005-05-31 2008-07-08 Agilent Technologies, Inc. Evanescent wave sensor with attached ligand
US20070050092A1 (en) * 2005-08-12 2007-03-01 Symyx Technologies, Inc. Event-based library process design
WO2007040500A1 (fr) * 2005-09-23 2007-04-12 Agilent Techologies, Inc. Procédé de génération in situ de molécules d'acide nucléique
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US8309710B2 (en) * 2009-06-29 2012-11-13 Agilent Technologies, Inc. Use of N-alkyl imidazole for sulfurization of oligonucleotides with an acetyl disulfide
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Also Published As

Publication number Publication date
KR950018035A (ko) 1995-07-22
US6825339B2 (en) 2004-11-30
HK1002330A1 (en) 1998-08-14
DE69404018T2 (de) 1998-01-15
KR100366862B1 (ko) 2003-12-01
EP0658566A1 (fr) 1995-06-21
US6623703B1 (en) 2003-09-23
ES2106479T3 (es) 1997-11-01
AU8043294A (en) 1995-06-22
EP0658566B1 (fr) 1997-07-02
US20030176690A1 (en) 2003-09-18
CA2138240C (fr) 2005-04-05
AU693487B2 (en) 1998-07-02
GR3024829T3 (en) 1998-01-30
US5869643A (en) 1999-02-09
FR2714061A1 (fr) 1995-06-23
DE69404018D1 (de) 1997-08-07
FR2714061B1 (fr) 1996-03-08
ATE154936T1 (de) 1997-07-15
JPH08239397A (ja) 1996-09-17
DK0658566T3 (da) 1998-02-02
TW349101B (en) 1999-01-01

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