CA2137041A1 - Assay for determining sensitivity to activated protein c - Google Patents

Assay for determining sensitivity to activated protein c

Info

Publication number
CA2137041A1
CA2137041A1 CA002137041A CA2137041A CA2137041A1 CA 2137041 A1 CA2137041 A1 CA 2137041A1 CA 002137041 A CA002137041 A CA 002137041A CA 2137041 A CA2137041 A CA 2137041A CA 2137041 A1 CA2137041 A1 CA 2137041A1
Authority
CA
Canada
Prior art keywords
activated protein
assay
sensitivity
activated
test sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002137041A
Other languages
French (fr)
Other versions
CA2137041C (en
Inventor
Katalin Varadi
Hans-Peter Schwarz
Hartmut Lang
Berta Moritz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oesterreichisches Institut fuer Haemoderivate
Original Assignee
Katalin Varadi
Hans-Peter Schwarz
Hartmut Lang
Berta Moritz
Immuno Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Katalin Varadi, Hans-Peter Schwarz, Hartmut Lang, Berta Moritz, Immuno Aktiengesellschaft filed Critical Katalin Varadi
Publication of CA2137041A1 publication Critical patent/CA2137041A1/en
Application granted granted Critical
Publication of CA2137041C publication Critical patent/CA2137041C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)
    • G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
    • G01N2333/96444—Factor X (3.4.21.6)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)
    • G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
    • G01N2333/9645—Factor IX (3.4.21.22)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)
    • G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
    • G01N2333/96461—Protein C (3.4.21.69)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
    • G01N2405/04—Phospholipids, i.e. phosphoglycerides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit

Abstract

An improved assay for determining sensitivity to activated protein C in test samples has been developed to ensure rapid and accurate evaluations. This assay is based on measuring the conversion, by activated factor VIII within a test sample, of added factor X to an activated form. The activated protein C sensitivity of the test sample is determined by the relative inhibition of factor X conversion as compared to a control. A test sample that has decreased sensitivity to activated protein C will show relatively low inhibition, and vice versa.
CA002137041A 1993-12-03 1994-11-30 Assay for determining sensitivity to activated protein c Expired - Fee Related CA2137041C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16087793A 1993-12-03 1993-12-03
US08/160,877 1993-12-03

Publications (2)

Publication Number Publication Date
CA2137041A1 true CA2137041A1 (en) 1995-06-04
CA2137041C CA2137041C (en) 2003-06-10

Family

ID=22578845

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002137041A Expired - Fee Related CA2137041C (en) 1993-12-03 1994-11-30 Assay for determining sensitivity to activated protein c

Country Status (6)

Country Link
US (1) US5643739A (en)
EP (1) EP0656424B1 (en)
JP (1) JPH07194397A (en)
AT (2) AT402824B (en)
CA (1) CA2137041C (en)
DE (1) DE59410041D1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE168781T1 (en) * 1993-01-29 1998-08-15 Bjoern Dahlbaeck NEW ANTICOAGULANT COFACTOR ACTIVITY
US5863896A (en) 1995-11-09 1999-01-26 Immuno Ag Evaluation of substances for altering and for increasing APC response
AT403289B (en) * 1996-01-30 1997-12-29 Immuno Ag METHOD FOR PRODUCING A STANDARDIZED PLASMAPOOL FOR DIAGNOSTIC PURPOSES
US6379975B1 (en) 1996-11-27 2002-04-30 T.A.C. Thrombosis And Coagulation Aktiebolag Methods and reagents for determining protein S
AUPP159698A0 (en) * 1998-02-02 1998-02-26 Life Therapeutics Limited Improved blood coagulation test
WO1999066939A1 (en) * 1998-06-23 1999-12-29 Marder Victor J Phospholipid vesicle-tissue factor complex preparations and methods of making and using same
US6855509B2 (en) * 2000-12-19 2005-02-15 Instrumentation Laboratory Company Protein S functional assay and kit therefor
AU2002231008A1 (en) * 2000-12-19 2002-07-01 Instrumentation Laboratory Co. Free analyte detection system
JP2007101362A (en) * 2005-10-04 2007-04-19 Hamamatsu Univ School Of Medicine Eia plate and its utilization method
JP2009198506A (en) * 2009-04-10 2009-09-03 Shino Test Corp Activity measuring method and activity measuring reagent for blood-clotting reaction inhibitory plasma protein
EP2712423A4 (en) 2011-02-25 2015-06-10 Wellstat Diagnostics Llc Assays for detecting enzymatic activity
JP6552286B2 (en) * 2015-06-05 2019-07-31 公立大学法人奈良県立医科大学 Method and reagent kit for obtaining information on complexing ability of coagulation factor VIII

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3407280A1 (en) * 1984-02-28 1985-09-19 Gödecke AG, 1000 Berlin TEST SET FOR DETERMINING THE ACTIVATED PARTIAL THROMBOPLASTIN TIME (PTT) WITH INCREASED HEPARINE SENSITIVITY
SE464135B (en) * 1989-07-14 1991-03-11 Kabivitrum Ab PROCEDURE FOR DETERMINING THE FUNCTIONAL ACTIVITY OF FREE PROTEIN S OR PROTEIN C IN A PLASMA TEST
US5169786A (en) * 1989-12-19 1992-12-08 Ortho Diagnostic Systems, Inc. Method of determining levels of extrinsic and intrinsic clotting factors and protein c
AT395597B (en) * 1991-01-25 1993-01-25 Immuno Ag REAGENT FOR DETERMINING FACTOR VIII ACTIVITY
JP2562000B2 (en) * 1991-11-13 1996-12-11 ダールベツク，ビヨルン Method for diagnosing blood coagulation disorders
US5308756A (en) * 1991-11-20 1994-05-03 Baxter Diagnostics Inc. Protein S chromogenic assay

Also Published As

Publication number Publication date
EP0656424A3 (en) 1996-01-31
DE59410041D1 (en) 2002-03-21
ATA222394A (en) 1997-01-15
EP0656424A2 (en) 1995-06-07
AT402824B (en) 1997-09-25
CA2137041C (en) 2003-06-10
EP0656424B1 (en) 2002-02-06
ATE213023T1 (en) 2002-02-15
US5643739A (en) 1997-07-01
JPH07194397A (en) 1995-08-01

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