CA2120629A1 - Production of gamma linolenic acid by a .delta.6-desaturase - Google Patents

Production of gamma linolenic acid by a .delta.6-desaturase

Info

Publication number
CA2120629A1
CA2120629A1 CA002120629A CA2120629A CA2120629A1 CA 2120629 A1 CA2120629 A1 CA 2120629A1 CA 002120629 A CA002120629 A CA 002120629A CA 2120629 A CA2120629 A CA 2120629A CA 2120629 A1 CA2120629 A1 CA 2120629A1
Authority
CA
Canada
Prior art keywords
delta
desaturase
production
linolenic acid
gamma linolenic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002120629A
Other languages
French (fr)
Other versions
CA2120629C (en
Inventor
Terry L. Thomas
Avutu S. Reddy
Michael Nuccio
Georges L. Freyssinet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer SAS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2120629A1 publication Critical patent/CA2120629A1/en
Application granted granted Critical
Publication of CA2120629C publication Critical patent/CA2120629C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19003—Linoleoyl-CoA desaturase (1.14.19.3)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Fats And Perfumes (AREA)

Abstract

Linoieic acid is converted into .gamma.-linolenic acid by the enzyme .DELTA.6-desaturase. The present invention is directed to an isolated nucleic acid comprising the .DELTA.6-desaturase gene. More particularly, the isolated nucleic acid comprises the promoter, coding region and termination regions of the .DELTA.6-desaturase gene. The present invention provides recombinant constructions comprising the .DELTA.6-desaturase coding region in functional combination with heterologous regulatory sequences. The nucleic acids and recombinant constructions of the instant invention are useful in the production of GLA in transgenic organisms.
CA002120629A 1991-10-10 1992-10-13 Production of gamma linolenic acid by a .delta.6-desaturase Expired - Lifetime CA2120629C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US77447591A 1991-10-10 1991-10-10
FR774,475 1991-10-10
US81791992A 1992-01-08 1992-01-08
FR817,919 1992-01-08
PCT/US1992/008746 WO1993006712A1 (en) 1991-10-10 1992-10-13 Production of gamma linolenic acid by a δ6-desaturase

Publications (2)

Publication Number Publication Date
CA2120629A1 true CA2120629A1 (en) 1993-04-15
CA2120629C CA2120629C (en) 2009-02-17

Family

ID=27118889

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002120629A Expired - Lifetime CA2120629C (en) 1991-10-10 1992-10-13 Production of gamma linolenic acid by a .delta.6-desaturase

Country Status (23)

Country Link
US (3) US5552306A (en)
EP (1) EP0666918B1 (en)
JP (1) JP3537434B2 (en)
KR (1) KR100316068B1 (en)
CN (2) CN1053469C (en)
AT (1) ATE284961T1 (en)
AU (1) AU667848B2 (en)
BG (1) BG61791B1 (en)
BR (1) BR9206613A (en)
CA (1) CA2120629C (en)
CZ (1) CZ285471B6 (en)
DE (1) DE69233459T2 (en)
DK (1) DK0666918T3 (en)
ES (1) ES2231767T3 (en)
HU (1) HU217328B (en)
IL (1) IL103407A (en)
MX (1) MX9205820A (en)
NZ (1) NZ244685A (en)
PH (1) PH31293A (en)
RO (1) RO113256B1 (en)
RU (1) RU2152996C2 (en)
UA (1) UA43314C2 (en)
WO (1) WO1993006712A1 (en)

Families Citing this family (119)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614393A (en) * 1991-10-10 1997-03-25 Rhone-Poulenc Agrochimie Production of γ-linolenic acid by a Δ6-desaturase
US20070130654A1 (en) * 1991-10-10 2007-06-07 Thomas Terry L Production of gamma linolenic acid by a delta6-desaturase
US6872872B1 (en) 1992-11-17 2005-03-29 E. I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants
US6372965B1 (en) 1992-11-17 2002-04-16 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants
WO1994018337A1 (en) * 1993-02-05 1994-08-18 Monsanto Company Altered linolenic and linoleic acid content in plants
US5639645A (en) * 1993-09-22 1997-06-17 Mitsubishi Corporation Recombinant Δ9 desaturase and a gene encoding the same
US6310194B1 (en) * 1994-09-26 2001-10-30 Carnegie Institution Of Washington Plant fatty acid hydroxylases
US5965793A (en) 1995-09-20 1999-10-12 Monsanto Company, Inc. Strong early seed-specific gene regulatory region
US5959175A (en) * 1997-04-09 1999-09-28 Thomas; Terry L. Sunflower albumin 5' regulatory region for the modification of plant seed lipid composition
US5977436A (en) 1997-04-09 1999-11-02 Rhone Poulenc Agrochimie Oleosin 5' regulatory region for the modification of plant seed lipid composition
US6075183A (en) * 1997-04-11 2000-06-13 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
US5968809A (en) 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US7745694B1 (en) 1997-04-11 2010-06-29 Monsanto Technology Llc Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants
US6432684B1 (en) 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
US6051754A (en) * 1997-04-11 2000-04-18 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
TR199902474T2 (en) * 1997-04-11 2000-02-21 Calgene Llc Methods and compositions for the synthesis of long chain polyunsaturated fatty acids in plants.
US5972664A (en) 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US6428990B1 (en) 1997-04-11 2002-08-06 Abbott Laboratories Human desaturase gene and uses thereof
US6080911A (en) * 1997-04-15 2000-06-27 Ohio University Mice models of growth hormone insensitivity
ES2275301T3 (en) * 1997-04-15 2007-06-01 Commonwealth Scientific And Industrial Research Organisation VEGETABLE ACID FAT ACID GENES AND THE USE OF THESE GENES.
US6100450A (en) * 1997-10-22 2000-08-08 Rhone-Poulenc Agrochimie Seed specific promoters based on arabidopsis genes
CA2329159A1 (en) * 1998-05-29 1999-12-02 Bruce Kelder Compositions and methods for the synthesis of fatty acids, their derivatives and downstream products
DE69937050T2 (en) * 1998-06-12 2008-05-29 Calgene Llc, Davis POLYUNG-SATURATED FATTY ACIDS IN PLANTS
US6913916B1 (en) 1998-09-02 2005-07-05 Abbott Laboratories Elongase genes and uses thereof
US6677145B2 (en) 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
US6403349B1 (en) 1998-09-02 2002-06-11 Abbott Laboratories Elongase gene and uses thereof
US20030163845A1 (en) * 1998-09-02 2003-08-28 Pradip Mukerji Elongase genes and uses thereof
AU776447B2 (en) 1999-06-07 2004-09-09 Basf Plant Science Gmbh Delta6-acetylenase and delta6-desaturase from ceratodon purpureus
DE10030976A1 (en) 2000-06-30 2002-01-10 Basf Ag Production of unsaturated fatty acids, useful e.g. in nutrition, cosmetics or pharmaceuticals, in organisms transformed with Physcomitrella patens delta-6-desaturase nucleic acid
US7070970B2 (en) 1999-08-23 2006-07-04 Abbott Laboratories Elongase genes and uses thereof
GB9929897D0 (en) * 1999-12-18 2000-02-09 Slabas Antoni R Improvements in or relating to conjugated fatty acids and related compounds
FR2803592A1 (en) 2000-01-06 2001-07-13 Aventis Cropscience Sa NOVEL DERIVATIVES OF 3-HYDROXYPICOLINIC ACID, PROCESS FOR THEIR PREPARATION AND FUNGICIDAL COMPOSITIONS CONTAINING SAME
FR2810994B1 (en) * 2000-06-30 2004-03-12 Biogemma Fr NUCLEIC ACIDS ENCODING A PLANT PHOSPHATASE MIPP TYPE WITH PHYTASIC ACTIVITY AND APPLICATIONS
EP1197550A3 (en) * 2000-08-25 2002-11-20 Pfizer Products Inc. Methods and compositions for diagnosing and treating disorders involving angiogenesis
EP1911837B1 (en) 2000-09-28 2011-05-25 Bioriginal Food & Science Corp. FAD5-2 fatty acid desaturase family member and uses thereof
FR2815969B1 (en) 2000-10-30 2004-12-10 Aventis Cropscience Sa TOLERANT PLANTS WITH HERBICIDES BY METABOLIC BYPASS
DE10102337A1 (en) 2001-01-19 2002-07-25 Basf Plant Science Gmbh Preparing ester containing polyunsaturated fatty acids, useful e.g. in animal or human nutrition, by transforming organism with desaturase gene from Phaeodactylum tricornatum
JP4347572B2 (en) * 2001-01-25 2009-10-21 アボット・ラボラトリーズ Desaturase gene and its use
US6635451B2 (en) 2001-01-25 2003-10-21 Abbott Laboratories Desaturase genes and uses thereof
US7045683B2 (en) * 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
EP1392278A4 (en) * 2001-05-14 2005-05-04 Martek Biosciences Corp Production and use of a polar lipid-rich fraction containing stearidonic acid and gamma linolenic acid from plant seeds and microbes
US20050129739A1 (en) * 2001-05-14 2005-06-16 Gerhard Kohn Production and use of a polar lipid-rich fraction containing omega-3 and/or omega-6 highly unsaturated fatty acids from microbes, genetically modified plant seeds and marine organisms
EP1472357B1 (en) 2002-01-30 2009-12-16 BASF Plant Science GmbH Method for producing polyunsaturated fatty acids by means of a new elongase-gene
US7211656B2 (en) * 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
GB2385852A (en) 2002-02-27 2003-09-03 Rothamsted Ex Station Delta 6-desaturases from Primulaceae
US20040025202A1 (en) * 2002-03-15 2004-02-05 Laurie Cathy C. Nucleic acid molecules associated with oil in plants
DE10219203A1 (en) 2002-04-29 2003-11-13 Basf Plant Science Gmbh Process for the production of polyunsaturated fatty acids in plants
EP2216405A1 (en) 2002-05-03 2010-08-11 Monsanto Technology LLC Speed specific USP promoters for expressing genes in plants
FR2848064B1 (en) * 2002-12-06 2006-01-13 Bayer Cropscience Sa FERTILIZED TRANSPLASTOMIC LEGUMINOUS PLANTS
FR2848570B1 (en) 2002-12-12 2005-04-01 Bayer Cropscience Sa EXPRESSION CASSETTE ENCODING A 5-ENOL PYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE (EPSPS) AND HERBICIDE TOLERANT PLANTS CONTAINING THE SAME
US7078234B2 (en) 2002-12-18 2006-07-18 Monsanto Technology Llc Maize embryo-specific promoter compositions and methods for use thereof
BR0317304A (en) 2002-12-19 2005-11-08 Univ Bristol Process for the production of compounds, isolated nucleic acid sequence, amino acid sequence, gene construct, vector, and, organism
US7537920B2 (en) 2003-02-27 2009-05-26 Basf Plant Science Gmbh Method for the production of polyunsaturated fatty acids
EP1613730A4 (en) 2003-03-28 2007-12-05 Monsanto Technology Llc Novel plant promoters for use in early seed development
WO2004087902A2 (en) 2003-03-31 2004-10-14 University Of Bristol Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
AU2004227075B8 (en) 2003-04-08 2009-07-16 Basf Plant Science Gmbh Delta-4 Desaturases from Euglena gracilis, expressing plants, and oils containing PUFA
EP2172536B1 (en) 2003-08-01 2016-05-04 BASF Plant Science GmbH Method for producing polyunsaturated fatty acids in transgenic organisms
US11952581B2 (en) 2003-08-01 2024-04-09 Basf Plant Science Gmbh Process for the production of polyunsaturated fatty acids in transgenic organisms
DE602004021001D1 (en) 2003-08-21 2009-06-18 Monsanto Technology Llc FATTY ACID SEEDURASES FROM PRIMULA
CA2450000A1 (en) * 2003-12-18 2005-06-18 Alberta Research Council Inc. Method of creating plants with reduced level of saturated fatty acid in seed oil
US7777098B2 (en) 2004-02-27 2010-08-17 Basf Plant Science Gmbh Method for producing unsaturated ω-3-fatty acids in transgenic organisms
CN1930277B (en) 2004-02-27 2011-02-02 巴斯福植物科学有限公司 Method for producing polyunsaturated fatty acids in transgenic plants
US20050220901A1 (en) * 2004-03-22 2005-10-06 Huttenbauer Samuel Jr Methods of pharmaceutical separation from plants
US8378186B2 (en) 2004-04-16 2013-02-19 Monsanto Technology Llc Expression of fatty acid desaturases in corn
CN103451246B (en) 2004-04-22 2018-02-16 联邦科学技术研究组织 With recombinant cell synthesis of long-chain polyunsaturated fatty acids
BRPI0510132A (en) 2004-04-22 2007-10-02 Commw Scient Ind Res Org long chain polyunsaturated fatty acid synthesis by recombinant cells
US7138391B2 (en) * 2004-08-09 2006-11-21 Silverbrook Research Pty Ltd Hydrophilizable and hydrophilic cyanine dyes
US7456270B2 (en) * 2004-09-01 2008-11-25 Abbott Laboratories Δ6-desaturase genes and uses thereof
AU2006217847B2 (en) 2005-02-26 2011-04-07 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
DE102005013779A1 (en) 2005-03-22 2006-09-28 Basf Plant Science Gmbh Process for the preparation of polyunsaturated C20 and C22 fatty acids having at least four double bonds in transgenic plants
CA2606220A1 (en) 2005-04-19 2006-12-21 Basf Plant Science Gmbh Starchy-endosperm and/or germinating embryo-specific expression in mono-cotyledonous plants
AU2006237346B2 (en) 2005-04-20 2011-04-07 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
CA2607263A1 (en) 2005-05-10 2006-11-16 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
NZ563676A (en) 2005-05-23 2011-01-28 Arcadia Biosciences Inc Safflower with elevated gamma-linolenic acid
DE102005038036A1 (en) 2005-08-09 2007-02-15 Basf Plant Science Gmbh Process for the preparation of arachidonic acid and / or eicosapentaenoic acid in transgenic crops
GB2431158A (en) 2005-10-13 2007-04-18 Rothamsted Res Ltd Process for the production of arachidonic and/or eicosapentaenoic acid
DE102005052551A1 (en) 2005-11-02 2007-05-16 Rothamsted Res Harpenden Process for the preparation of γ-linolenic acid and / or stearidonic acid in transgenic Brassicaceae and Linaceae
EP1806398A1 (en) * 2006-01-04 2007-07-11 Monsanto S.A.S. Fad-2 mutants and high oleic plants
GB0603160D0 (en) 2006-02-16 2006-03-29 Rothamsted Res Ltd Nucleic acid
AR059376A1 (en) 2006-02-21 2008-03-26 Basf Plant Science Gmbh PROCEDURE FOR THE PRODUCTION OF POLYINSATURATED FATTY ACIDS
US8629195B2 (en) 2006-04-08 2014-01-14 Bayer Materialscience Ag Production of polyurethane foams
ES2366297T3 (en) 2006-07-19 2012-01-13 Monsanto Technology, Llc DESATURASAS OF FATTY ACIDS OF SWEDEN TETRASELMIS.
US9029111B2 (en) 2006-08-24 2015-05-12 Basf Plant Science Gmbh Isolation and characterization of a novel pythium omega 3 desaturase with specificity to all omega 6 fatty acids longer than 18 carbon chains
BRPI0716075A2 (en) 2006-08-29 2013-08-06 Commw Scient Ind Res Org Fatty Acid Synthesis
EP2177605B1 (en) 2006-10-06 2014-12-10 BASF Plant Science GmbH Delta-5 Desaturases and method for producing polyunsaturated fatty acids in transgenic non-human organisms
WO2008099013A1 (en) 2007-02-16 2008-08-21 Basf Plant Science Gmbh Nucleic acid sequences for regulation of embryo-specific expression in monocotyledonous plants
MY154965A (en) 2007-06-01 2015-08-28 Solazyme Inc Production of oil in microorganisms
EP2176416B1 (en) 2007-07-31 2016-03-16 BASF Plant Science GmbH Elongases and methods for producing polyunsaturated fatty acids in transgenic organisms
US20130323801A1 (en) * 2007-11-01 2013-12-05 Wake Forest University School Of Medicine Compositions, Methods, and Kits for Polyunsaturated Fatty Acids from Microalgae
AU2008337600A1 (en) 2007-12-14 2009-06-25 Basf Plant Science Gmbh Promoters from brassica napus for seed specific gene expression
AU2009242130B2 (en) 2008-04-30 2013-09-19 Rothamsted Research Ltd. Desaturase and method for the production of polyunsaturated fatty acids in transgenic organisms
AU2009265795B2 (en) 2008-07-01 2014-12-11 Basf Plant Science Gmbh Promoters from Brassica napus for seed specific gene expression
DE112009002048T5 (en) 2008-08-26 2012-01-26 Basf Plant Science Gmbh Nucleic acid encoding desaturases and modified plant oil
CN107858204B (en) 2008-11-18 2021-10-29 联邦科学技术研究组织 Enzymes and methods for producing omega-3 fatty acids
MX2011005630A (en) 2008-11-28 2011-09-28 Solazyme Inc Manufacturing of tailored oils in recombinant heterotrophic microorganisms.
DE112009003708T5 (en) 2008-12-12 2012-09-13 Basf Plant Science Gmbh Desaturases and methods of producing polyunsaturated fatty acids in transgenic organisms
AR074941A1 (en) 2009-01-07 2011-02-23 Bayer Cropscience Sa TRANSPLASTOMIC PLANTS EXEMPTED FROM SELECTOR MARKER
CN104630220A (en) 2009-04-17 2015-05-20 巴斯夫植物科学有限公司 Plant promoter operable in endosperm and uses thereof
EP2421976B1 (en) 2009-04-22 2015-09-16 BASF Plant Science Company GmbH Whole seed specific promoter
AU2010247438B2 (en) 2009-05-13 2015-01-29 Basf Plant Science Company Gmbh Acyltransferases and uses thereof in fatty acid production
CN102471777B (en) 2009-07-10 2014-09-24 巴斯夫植物科学有限公司 Expression cassettes for endosperm-specific expression in plants
US8188335B2 (en) 2009-07-17 2012-05-29 Abbott Laboratories Δ9-elongase for production of polyunsaturated fatty acid-enriched oils
CA2782290A1 (en) 2009-12-03 2011-06-09 Basf Plant Science Company Gmbh Expression cassettes for embryo-specific expression in plants
CA2788052C (en) 2010-02-02 2020-02-04 Bayer Cropscience Ag Soybean transformation using hppd inhibitors as selection agents
MX339639B (en) 2010-05-28 2016-06-02 Solazyme Inc * Tailored oils produced from recombinant heterotrophic microorganisms.
NO2585603T3 (en) 2010-06-25 2018-05-19
SG10201509035WA (en) 2010-11-03 2015-12-30 Solazyme Inc Microbial Oils With Lowered Pour Points, Dielectric Fluids Produced Therefrom, And Related Methods
MX344012B (en) 2011-02-02 2016-12-02 Terravia Holdings Inc Tailored oils produced from recombinant oleaginous microorganisms.
ES2769448T3 (en) 2012-04-12 2020-06-25 Rothamsted Res Limited Production of long-chain omega-3 polyunsaturated fatty acids
EP3550025A1 (en) 2012-04-18 2019-10-09 Corbion Biotech, Inc. Tailored oils
US9719114B2 (en) 2012-04-18 2017-08-01 Terravia Holdings, Inc. Tailored oils
ES2636487T3 (en) 2012-06-15 2017-10-05 Commonwealth Scientific And Industrial Research Organisation Production of long chain polyunsaturated fatty acids in plant cells
GB201217524D0 (en) 2012-10-01 2012-11-14 Rothamsted Res Ltd Recombinant organisms
WO2014176515A2 (en) * 2013-04-26 2014-10-30 Solazyme, Inc. Low polyunsaturated fatty acid oils and uses thereof
AU2014331605A1 (en) 2013-10-04 2016-05-12 Corbion Biotech, Inc. Tailored oils
EA037817B1 (en) 2013-12-18 2021-05-25 Коммонвелт Сайнтифик Энд Индастриэл Рисерч Организэйшн Extracted plant lipid comprising long chain polyunsaturated fatty acids
EP3160482A4 (en) 2014-06-27 2018-02-14 Commonwealth Scientific and Industrial Research Organisation Lipid comprising docosapentaenoic acid
BR112017000414A2 (en) 2014-07-10 2017-11-07 Terravia Holdings Inc ketoacyl acp synthase genes and their uses
KR102054762B1 (en) 2018-03-06 2020-01-22 대한민국(관리청: 특허청장, 승계청: 농촌진흥청장) FATTY ACID CHAIN ELONGASE GENE FROM Berryteuthis magister AND USES THEREOF

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4736866B1 (en) * 1984-06-22 1988-04-12 Transgenic non-human mammals
NZ221259A (en) * 1986-07-31 1990-05-28 Calgene Inc Seed specific transcriptional regulation
CA1333397C (en) * 1987-11-04 1994-12-06 Jean Bernard Ducep Fluorinated arachidonic acid derivatives
JP3364616B2 (en) * 1989-02-24 2003-01-08 モンサント テクノロジー エルエルシー Synthetic plant genes and preparation methods
ATE241007T1 (en) * 1990-03-16 2003-06-15 Calgene Llc DNAS CODING FOR PLANT DESATURASES AND THEIR APPLICATIONS

Also Published As

Publication number Publication date
IL103407A (en) 1999-09-22
HUT69781A (en) 1995-09-28
EP0666918B1 (en) 2004-12-15
NZ244685A (en) 1994-06-27
CN1072722A (en) 1993-06-02
RO113256B1 (en) 1998-05-29
RU2152996C2 (en) 2000-07-20
BG98695A (en) 1995-05-31
KR100316068B1 (en) 2002-11-13
CA2120629C (en) 2009-02-17
DK0666918T3 (en) 2005-03-14
JPH07503605A (en) 1995-04-20
EP0666918A4 (en) 1995-03-17
MX9205820A (en) 1993-04-01
US5689050A (en) 1997-11-18
BG61791B1 (en) 1998-06-30
CN1053469C (en) 2000-06-14
DE69233459D1 (en) 2005-01-20
BR9206613A (en) 1995-04-11
WO1993006712A1 (en) 1993-04-15
US5552306A (en) 1996-09-03
HU9401007D0 (en) 1994-08-29
CZ285471B6 (en) 1999-08-11
DE69233459T2 (en) 2005-12-15
HU217328B (en) 1999-12-28
CN1174236A (en) 1998-02-25
US5663068A (en) 1997-09-02
CZ81794A3 (en) 1995-09-13
EP0666918A1 (en) 1995-08-16
AU2881292A (en) 1993-05-03
UA43314C2 (en) 2001-12-17
AU667848B2 (en) 1996-04-18
PH31293A (en) 1998-07-06
JP3537434B2 (en) 2004-06-14
CN1121499C (en) 2003-09-17
ATE284961T1 (en) 2005-01-15
IL103407A0 (en) 1993-03-15
ES2231767T3 (en) 2005-05-16

Similar Documents

Publication Publication Date Title
CA2120629A1 (en) Production of gamma linolenic acid by a .delta.6-desaturase
WO1996021022A3 (en) Production of gamma linolenic acid by a δ6-desaturase
de Boer et al. Biased codon usage: An exploration of its role in optimization of translation.
AU2443488A (en) Dna-molecules coding for fmdh control regions and structured gene for a protein having fmdh-activity and their uses
WO1991000920A3 (en) Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
DE3686217D1 (en) ALKALINE PROTEASE, METHOD FOR PRODUCING HYBRID VECTORS AND GENETICALLY TRANSFORMED MICROORGANISMS.
CA2166685A1 (en) An enzyme with protease activity
GR3036011T3 (en) Genetic sequences encoding flavonoid pathway enzymes and uses therefor.
AU3828889A (en) Method for the preparation of a yeast extract said yeast extract, its use as a food flavour and a food composition comprising the yeast extract
DE68926893D1 (en) Process for the production of clavulanic acid
AU4709889A (en) Recombinant dna expression vectors and dna compounds that encode isopenicillin n epimerase (racemase) activity
WO1997011189A3 (en) Plant glutathione s-transferase promoters
GR3020786T3 (en) Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N epimerase activity
DE69125541T2 (en) Process for the enzymatic production of 7-aminocephalosporic acid
AU7004298A (en) Process for the preparation of alpha-acetolactic acid
CA2068614A1 (en) Esterase genes, esterase, recombinant plasmids and transformants containing the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters using said transformants
DK0733704T3 (en) Controllable expression system
EP0976826A4 (en) Gene encoding recombinant trehalose phosphorylase, vector containing the gene, transformant transformed by the gene, and method for producing recombinant trehalose phosphorylase with the use of the transformant
WO1994020631A3 (en) Production of glycolate oxidase in methylotrophic yeast

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry
MKEX Expiry

Effective date: 20121015

MKEX Expiry

Effective date: 20121015