CA2116239A1 - Gene probe biosensor method - Google Patents
Gene probe biosensor methodInfo
- Publication number
- CA2116239A1 CA2116239A1 CA002116239A CA2116239A CA2116239A1 CA 2116239 A1 CA2116239 A1 CA 2116239A1 CA 002116239 A CA002116239 A CA 002116239A CA 2116239 A CA2116239 A CA 2116239A CA 2116239 A1 CA2116239 A1 CA 2116239A1
- Authority
- CA
- Canada
- Prior art keywords
- dna
- rna
- gene probe
- biosensor method
- probe biosensor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/7703—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Abstract
The present invention relates to a method for the detection, identification and/or quantification of plant or animal tissues, microorganisms or cell free RNA or DNA and to detector apparatus adapted for performing said method. The method particularly uses Total Internal Reflection Fluorescence (TIRF) to measure hybridization of analyte RNA or DNA with RNA or DNA
that is associated with an evanescent wave detector waveguide.
that is associated with an evanescent wave detector waveguide.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9119735.0 | 1991-09-16 | ||
GB919119735A GB9119735D0 (en) | 1991-09-16 | 1991-09-16 | Gene probe biosensor method |
PCT/GB1992/001698 WO1993006241A1 (en) | 1991-09-16 | 1992-09-16 | Gene probe biosensor method |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2116239A1 true CA2116239A1 (en) | 1993-04-01 |
CA2116239C CA2116239C (en) | 2008-11-18 |
Family
ID=10701461
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002116239A Expired - Fee Related CA2116239C (en) | 1991-09-16 | 1992-09-16 | Gene probe biosensor method |
Country Status (20)
Country | Link |
---|---|
US (1) | US5750337A (en) |
EP (1) | EP0604506B1 (en) |
JP (1) | JP3324607B2 (en) |
KR (1) | KR100249288B1 (en) |
AT (1) | ATE174968T1 (en) |
AU (1) | AU665369B2 (en) |
BG (1) | BG62337B1 (en) |
CA (1) | CA2116239C (en) |
CZ (1) | CZ286927B6 (en) |
DE (1) | DE69227997T2 (en) |
DK (1) | DK0604506T3 (en) |
ES (1) | ES2125272T3 (en) |
FI (1) | FI108463B (en) |
GB (2) | GB9119735D0 (en) |
GR (1) | GR3029722T3 (en) |
HU (1) | HU218596B (en) |
NO (1) | NO315380B1 (en) |
RU (1) | RU2116349C1 (en) |
SK (1) | SK280713B6 (en) |
WO (1) | WO1993006241A1 (en) |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07505297A (en) * | 1992-04-06 | 1995-06-15 | アボツト・ラボラトリーズ | Method and apparatus for detecting nucleic acids or analytes using total internal reflection |
WO1995026416A1 (en) * | 1994-03-25 | 1995-10-05 | Research Corporation Technologies, Inc. | Nucleic acid biosensor diagnostics |
US5599668A (en) * | 1994-09-22 | 1997-02-04 | Abbott Laboratories | Light scattering optical waveguide method for detecting specific binding events |
CA2207629A1 (en) * | 1994-12-22 | 1996-06-27 | Abbott Laboratories | Methods of immobilizing oligonucleotides to solid support materials and methods of using support bound oligonucleotides |
US5690894A (en) * | 1995-05-23 | 1997-11-25 | The Regents Of The University Of California | High density array fabrication and readout method for a fiber optic biosensor |
SE9502608D0 (en) * | 1995-07-14 | 1995-07-14 | Pharmacia Biosensor Ab | Method for nucleic acid sequencing |
WO1997032212A1 (en) * | 1996-03-01 | 1997-09-04 | Beckman Instruments, Inc. | System for simultaneously conducting multiple ligand binding assays |
US5832165A (en) * | 1996-08-28 | 1998-11-03 | University Of Utah Research Foundation | Composite waveguide for solid phase binding assays |
US6503711B1 (en) | 1997-06-18 | 2003-01-07 | Ulrich J. Krull | Nucleic acid biosensor diagnostics |
DE19730359A1 (en) * | 1997-07-15 | 1999-01-21 | Boehringer Mannheim Gmbh | Integrated method and system for amplification and detection of nucleic acids |
PT1017848E (en) * | 1997-07-28 | 2003-02-28 | Medical Biosystems Ltd | ANALYSIS OF NUCLEIC ACID SEQUENCES |
GB2335035B (en) * | 1998-03-03 | 2003-05-28 | Brax Genomics Ltd | Screening for functional antisense agents |
GB9805935D0 (en) * | 1998-03-19 | 1998-05-13 | Ciba Geigy Ag | Organic compounds |
EP0957354A3 (en) * | 1998-03-20 | 2000-03-22 | IA Inc. | Method and apparatus for measuring binding between biological molecules |
US6429023B1 (en) | 1998-07-20 | 2002-08-06 | Shayda Technologies, Inc. | Biosensors with polymeric optical waveguides |
CA2644688C (en) * | 1999-04-20 | 2012-11-13 | Kankyo Engineering Co., Ltd. | Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analysing data obtained by the method |
US7205347B2 (en) * | 2000-10-19 | 2007-04-17 | Trans Photonics, Llc. | Substituted-polyaryl chromophoric compounds |
AR031640A1 (en) * | 2000-12-08 | 2003-09-24 | Applied Research Systems | ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS IN A SOLID SUPPORT |
US6597499B2 (en) | 2001-01-25 | 2003-07-22 | Olympus Optical Co., Ltd. | Total internal reflection fluorescence microscope having a conventional white-light source |
US20040241699A1 (en) * | 2001-10-04 | 2004-12-02 | Giovanni Zocchi | Detection of polynucleotide hybridization |
DE102004016247A1 (en) * | 2004-04-02 | 2005-10-20 | Chromeon Gmbh | Solid support systems for homogeneous detection of interactions of biomolecules without washing steps |
US20060088844A1 (en) * | 2004-10-22 | 2006-04-27 | Honeywell International Inc. | Real-time PCR microarray based on evanescent wave biosensor |
GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
GB0524069D0 (en) * | 2005-11-25 | 2006-01-04 | Solexa Ltd | Preparation of templates for solid phase amplification |
WO2007105786A1 (en) * | 2006-03-16 | 2007-09-20 | National University Corporation Akita University | Method of detecting nucleic acid and nucleic acid detection kit |
US20080009420A1 (en) * | 2006-03-17 | 2008-01-10 | Schroth Gary P | Isothermal methods for creating clonal single molecule arrays |
US7824902B2 (en) * | 2006-11-03 | 2010-11-02 | Mark Selker | Optical interface for disposable bioreactors |
EP1935496A1 (en) | 2006-12-22 | 2008-06-25 | Eppendorf Array Technologies SA | Device and/or method for the detection of amplified nucleotides sequences on micro-arrays |
CN101589303B (en) * | 2007-01-17 | 2012-01-11 | 霍尼韦尔国际公司 | Microarray reader based on evanescent wave detection and method of reading a microarray |
WO2008093098A2 (en) | 2007-02-02 | 2008-08-07 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
WO2009079857A1 (en) * | 2007-12-24 | 2009-07-02 | Honeywell International Inc. | A reactor for the quantitative analysis of nucleic acids |
US9005931B2 (en) | 2007-12-24 | 2015-04-14 | Honeywell International Inc. | Programmable oligonucleotide micro array |
RU2508536C2 (en) | 2008-02-04 | 2014-02-27 | Конинклейке Филипс Электроникс Н.В. | Fluorescence detection system and method |
CN102791882A (en) | 2010-01-20 | 2012-11-21 | 霍尼韦尔国际公司 | Reactor for quantitative analysis of nucleic acids |
EP2726633B1 (en) * | 2011-06-30 | 2018-01-24 | Bioquanta Sa | Process and apparatus for quantifying nucleic acid in a sample |
RU2755151C2 (en) | 2016-10-27 | 2021-09-13 | Ванадис Дайэгностикс | Method for obtaining products by means of amplification according to rolling ring type |
JP2022523799A (en) * | 2019-03-01 | 2022-04-26 | ヴィディア・ホールディングス・リミテッド | Improvements in or related to optical elements |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1180647A (en) * | 1981-07-17 | 1985-01-08 | Cavit Akin | Light-emitting polynucleotide hybridization diagnostic method |
US4582809A (en) * | 1982-06-14 | 1986-04-15 | Myron J. Block | Apparatus including optical fiber for fluorescence immunoassay |
EP0144914A3 (en) * | 1983-12-12 | 1986-08-13 | Miles Inc. | Hybridization assay employing labeled pairs of hybrid binding reagents |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US5242797A (en) * | 1986-03-21 | 1993-09-07 | Myron J. Block | Nucleic acid assay method |
EP0245206A1 (en) * | 1986-05-05 | 1987-11-11 | IntraCel Corporation | Analytical method for detecting and measuring specifically sequenced nucleic acid |
BE1000572A4 (en) * | 1987-05-20 | 1989-02-07 | Block Myron J | Appts. for analysing polynucleotide in liq. sample - using internal reflection cell coated with complementary single strand sequences and selective fluorescent dye |
AU622426B2 (en) * | 1987-12-11 | 1992-04-09 | Abbott Laboratories | Assay using template-dependent nucleic acid probe reorganization |
GB8902689D0 (en) * | 1989-02-07 | 1989-03-30 | Ici Plc | Assay method |
GB8910880D0 (en) * | 1989-05-11 | 1989-06-28 | Amersham Int Plc | Sequencing method |
GB8919411D0 (en) * | 1989-08-25 | 1989-10-11 | Amersham Int Plc | Assay method |
CA2032203C (en) * | 1989-12-29 | 2009-05-19 | Christine L. Brakel | Amplification capture assay |
EP0478319B1 (en) * | 1990-09-28 | 1997-04-02 | Kabushiki Kaisha Toshiba | Gene detection method |
USH1398H (en) * | 1990-12-28 | 1995-01-03 | Campbell; James R. | DNA-based fluourescent sensor |
-
1991
- 1991-09-16 GB GB919119735A patent/GB9119735D0/en active Pending
-
1992
- 1992-09-16 WO PCT/GB1992/001698 patent/WO1993006241A1/en active IP Right Grant
- 1992-09-16 CA CA002116239A patent/CA2116239C/en not_active Expired - Fee Related
- 1992-09-16 SK SK306-94A patent/SK280713B6/en not_active IP Right Cessation
- 1992-09-16 AT AT92919549T patent/ATE174968T1/en not_active IP Right Cessation
- 1992-09-16 HU HU9400770A patent/HU218596B/en not_active IP Right Cessation
- 1992-09-16 DK DK92919549T patent/DK0604506T3/en active
- 1992-09-16 GB GB9402823A patent/GB2274710B/en not_active Revoked
- 1992-09-16 JP JP50589793A patent/JP3324607B2/en not_active Expired - Fee Related
- 1992-09-16 AU AU25641/92A patent/AU665369B2/en not_active Ceased
- 1992-09-16 RU RU94019336A patent/RU2116349C1/en not_active IP Right Cessation
- 1992-09-16 KR KR1019940700843A patent/KR100249288B1/en not_active IP Right Cessation
- 1992-09-16 CZ CZ1994521A patent/CZ286927B6/en not_active IP Right Cessation
- 1992-09-16 DE DE69227997T patent/DE69227997T2/en not_active Expired - Lifetime
- 1992-09-16 US US08/196,185 patent/US5750337A/en not_active Expired - Lifetime
- 1992-09-16 ES ES92919549T patent/ES2125272T3/en not_active Expired - Lifetime
- 1992-09-16 EP EP92919549A patent/EP0604506B1/en not_active Expired - Lifetime
-
1994
- 1994-03-14 NO NO19940901A patent/NO315380B1/en not_active IP Right Cessation
- 1994-03-15 BG BG98660A patent/BG62337B1/en unknown
- 1994-03-15 FI FI941220A patent/FI108463B/en active
-
1999
- 1999-03-18 GR GR990400807T patent/GR3029722T3/en unknown
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2116239A1 (en) | Gene probe biosensor method | |
EP0794261A3 (en) | Methods for detection and discrimination of multiple analytes using fluorescent technology | |
FR2422956A1 (en) | METHOD OF DETECTION AND CHARACTERIZATION OF A NUCLEIC ACID OR OF A SEQUENCE OF THE SAME, AND ENZYMATIC REAGENT FOR THE IMPLEMENTATION OF THIS PROCESS | |
KR970707299A (en) | Compositions and Methods for the Simultaneous Detection and Quantification of Multiple Specific Nucleic Acid Sequences | |
CA2304260A1 (en) | Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method | |
WO1995006756A3 (en) | OLIGONUCLEOTIDES AND METHODS FOR THE DETECTION OF $i(CHLAMYDIA TRACHOMATIS) | |
JPH11510385A (en) | Detection of nucleic acids and nucleic acid units | |
AU4920097A (en) | Use of anti-embryonic hemoglobin antibodies to identify fetal cells | |
JP2005504543A (en) | An adaptive baseline algorithm for quantitative PCR | |
EP0892070A3 (en) | Integrated process and system for the amplification and detection of nucleic acids | |
WO1999054510A3 (en) | Quantitative analysis of gene expression | |
AU4663997A (en) | Detection and identification of non-polio enteroviruses | |
HK1068377A1 (en) | Analytical method and kit | |
EP0723156A3 (en) | Assay normalization method | |
CA2145719A1 (en) | Detection of Nucleic Acid Amplification | |
Larsson | Quantitation of in situ hybridization analysis | |
WO2003104490A3 (en) | Method for the simultaneous detection of hybridization and vaccination reactions | |
RU97100364A (en) | METHOD FOR DETECTING AND EVALUATING ANAEROBIC BACTERIA CONCENTRATION IN BIOLOGICAL SUBSTRATE | |
CA2211165A1 (en) | Methods for sensitive detection of reverse transcriptase | |
WO2000055370A3 (en) | Bar coding and indentifying nucleic acids using a limited number of probes and low stringency conditions |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |