CA2112130A1 - Screening Assay for the Detection of DNA-Binding Molecules - Google Patents
Screening Assay for the Detection of DNA-Binding MoleculesInfo
- Publication number
- CA2112130A1 CA2112130A1 CA2112130A CA2112130A CA2112130A1 CA 2112130 A1 CA2112130 A1 CA 2112130A1 CA 2112130 A CA2112130 A CA 2112130A CA 2112130 A CA2112130 A CA 2112130A CA 2112130 A1 CA2112130 A1 CA 2112130A1
- Authority
- CA
- Canada
- Prior art keywords
- dna
- sequence
- binding
- test
- assay
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
Abstract
The present invention defines an assay use-ful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA
test sequences. The assay is also useful for deter-mining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. The assay is a competition assay in which binding of a test mole-cule to a DNA test sequence changes the binding characteristics of a DNA-binding protein to its binding sequence. When such a test molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the ratio between unbound DNA and DNA:protein complexes. The assay is versatile in that any test sequence can be tested by placing the test sequence adjacent to a defined protein binding DNA screening sequence.
test sequences. The assay is also useful for deter-mining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. The assay is a competition assay in which binding of a test mole-cule to a DNA test sequence changes the binding characteristics of a DNA-binding protein to its binding sequence. When such a test molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the ratio between unbound DNA and DNA:protein complexes. The assay is versatile in that any test sequence can be tested by placing the test sequence adjacent to a defined protein binding DNA screening sequence.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US72361891A | 1991-06-27 | 1991-06-27 | |
US07/723,618 | 1991-06-27 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2112130A1 true CA2112130A1 (en) | 1993-01-07 |
CA2112130C CA2112130C (en) | 1996-08-06 |
Family
ID=24907008
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002112130A Expired - Fee Related CA2112130C (en) | 1991-06-27 | 1992-06-26 | Screening assay for the detection of dna-binding molecules |
Country Status (12)
Country | Link |
---|---|
US (5) | US5693463A (en) |
EP (2) | EP0593618B1 (en) |
JP (1) | JP3169610B2 (en) |
KR (1) | KR100235575B1 (en) |
AT (1) | ATE165397T1 (en) |
AU (1) | AU655839B2 (en) |
CA (1) | CA2112130C (en) |
DE (1) | DE69225227T2 (en) |
DK (1) | DK0593618T3 (en) |
ES (1) | ES2117050T3 (en) |
HK (1) | HK1010058A1 (en) |
WO (1) | WO1993000446A1 (en) |
Families Citing this family (108)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5849478A (en) * | 1986-08-14 | 1998-12-15 | Cashman; Daniel P. | Blocked-polymerase polynucleotide immunoassay method and kit |
US5723289A (en) * | 1990-06-11 | 1998-03-03 | Nexstar Pharmaceuticals, Inc. | Parallel selex |
DK0593618T3 (en) * | 1991-06-27 | 1998-11-23 | Genelabs Tech Inc | Screening assay for detection of DNA-binding molecules |
US5726014A (en) * | 1991-06-27 | 1998-03-10 | Genelabs Technologies, Inc. | Screening assay for the detection of DNA-binding molecules |
US5578444A (en) * | 1991-06-27 | 1996-11-26 | Genelabs Technologies, Inc. | Sequence-directed DNA-binding molecules compositions and methods |
US6017696A (en) * | 1993-11-01 | 2000-01-25 | Nanogen, Inc. | Methods for electronic stringency control for molecular biological analysis and diagnostics |
US8153602B1 (en) | 1991-11-19 | 2012-04-10 | Isis Pharmaceuticals, Inc. | Composition and methods for the pulmonary delivery of nucleic acids |
US5616461A (en) * | 1992-05-14 | 1997-04-01 | Dana-Farber Cancer Institute | Assay for antiviral activity using complex of herpesvirus origin of replication and cellular protein |
US5795714A (en) | 1992-11-06 | 1998-08-18 | Trustees Of Boston University | Method for replicating an array of nucleic acid probes |
US6436635B1 (en) | 1992-11-06 | 2002-08-20 | Boston University | Solid phase sequencing of double-stranded nucleic acids |
US6824976B1 (en) * | 1993-04-02 | 2004-11-30 | Rigel Pharmaceuticals, Inc. | Method for selective inactivation of viral replication |
US20030099945A1 (en) * | 1994-09-20 | 2003-05-29 | Invenux, Inc. | Parallel selex |
US20030104361A1 (en) * | 1997-09-29 | 2003-06-05 | Susan Weininger | Method of detection of nucleic acids with a specific sequence composition |
US5871902A (en) * | 1994-12-09 | 1999-02-16 | The Gene Pool, Inc. | Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids |
US7803529B1 (en) | 1995-04-11 | 2010-09-28 | Sequenom, Inc. | Solid phase sequencing of biopolymers |
US20060063193A1 (en) * | 1995-04-11 | 2006-03-23 | Dong-Jing Fu | Solid phase sequencing of double-stranded nucleic acids |
US5830655A (en) * | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
DE69627333T2 (en) | 1995-06-26 | 2004-02-12 | PerSeptive Biosystems, Inc., Framingham | AUTOMATED, CONTINUOUS, MULTI-DIMENSIONAL, HIGH-SPEED, MOLECULAR SELECTION AND ANALYSIS |
CA2203832A1 (en) | 1995-09-08 | 1997-03-13 | Jaime E. Arenas | Screen for compounds with affinity for rna |
AU6869896A (en) * | 1995-09-12 | 1997-04-01 | Auda Pharmaceuticals Aps | Actinomycin d analogues |
US6514746B1 (en) | 1995-09-15 | 2003-02-04 | Essential Therapeutics, Inc. | Staphylococcus aureus histidine protein kinase essential genes |
US6037123A (en) * | 1995-09-15 | 2000-03-14 | Microcide Pharmaceuticals, Inc. | Methods of screening for compounds active on Staphylococcus aureus target genes |
US5891643A (en) * | 1995-11-14 | 1999-04-06 | Abbott Laboratories | Use of nuclear magnetic resonance to design ligands to target biomolecules |
US5698401A (en) * | 1995-11-14 | 1997-12-16 | Abbott Laboratories | Use of nuclear magnetic resonance to identify ligands to target biomolecules |
US5989827A (en) * | 1995-11-14 | 1999-11-23 | Abbott Laboratories | Use of nuclear magnetic resonance to design ligands to target biomolecules |
CA2279675A1 (en) * | 1995-12-15 | 1997-06-16 | Enzo Therapeutics, Inc. | Property effecting and/or property exhibiting constructs for localizing a nucleic acid construct within a cell for therapeutic and diagnostic uses |
US5747256A (en) * | 1995-12-19 | 1998-05-05 | Beckman Instruments, Inc. | Homogeneous DNA probe titration assay |
US6602657B1 (en) | 1995-12-28 | 2003-08-05 | Tropix, Inc. | Multiple reporter gene assay |
WO1997024457A1 (en) | 1995-12-29 | 1997-07-10 | Guilford Pharmaceuticals Inc. | Method and kit for detection of multiple protein interactions |
US6090947A (en) | 1996-02-26 | 2000-07-18 | California Institute Of Technology | Method for the synthesis of pyrrole and imidazole carboxamides on a solid support |
US6635417B1 (en) | 1996-07-31 | 2003-10-21 | California Institute Of Technology | Complex formation between DSDNA and oligomer of cyclic heterocycles |
US6555692B1 (en) | 1996-02-26 | 2003-04-29 | California Institute Of Technology | Preparation and use of bifunctional molecules having DNA sequence binding specificity |
AU6757698A (en) | 1996-02-26 | 1998-10-30 | California Institute Of Technology | Stereochemical control of the dna binding affinity, sequence specificity, and orientation-preference of chiral hairpin polyamides in the minor groove |
AU734715B2 (en) | 1996-02-26 | 2001-06-21 | California Institute Of Technology | Improved polyamides for binding in the minor groove of double stranded DNA |
US6143901A (en) * | 1996-07-31 | 2000-11-07 | Genesoft, Inc. | Complex formation between dsDNA and pyrrole imidazole polyamides |
US6506906B1 (en) | 1996-02-26 | 2003-01-14 | California Institute Of Technology | Preparation and use of bifunctional molecules having DNA sequence binding specificity |
US5955590A (en) * | 1996-07-15 | 1999-09-21 | Worcester Foundation For Biomedical Research | Conjugates of minor groove DNA binders with antisense oligonucleotides |
US5998140A (en) * | 1996-07-31 | 1999-12-07 | The Scripps Research Institute | Complex formation between dsDNA and oligomer of cyclic heterocycles |
WO1998039484A1 (en) | 1997-03-05 | 1998-09-11 | Scriptgen Pharmaceuticals, Inc. | Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids |
US6586661B1 (en) * | 1997-06-12 | 2003-07-01 | North Carolina State University | Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid |
US5939261A (en) * | 1997-06-24 | 1999-08-17 | Sarnoff Corporation | Method for capturing a nucleic acid |
US5972650A (en) * | 1997-06-26 | 1999-10-26 | Brigham And Women's Hospital | Tetracycline repressor regulated mammalian cell transcription and viral replication switch |
DE69834038D1 (en) | 1997-07-01 | 2006-05-18 | Isis Pharmaceutical Inc | COMPOSITIONS AND METHOD FOR THE ADMINISTRATION OF OLIGONUCLEOTIDES OVER THE DISHES |
US6410228B1 (en) | 1997-07-14 | 2002-06-25 | Baylor College Of Medicine | Method for the identification of synthetic cell- or tissue- specific transcriptional regulatory regions |
US6326489B1 (en) | 1997-08-05 | 2001-12-04 | Howard Hughes Medical Institute | Surface-bound, bimolecular, double-stranded DNA arrays |
EP1015889A1 (en) * | 1997-09-12 | 2000-07-05 | Genelabs Technologies, Inc. | dsRNA/dsRNA-BINDING PROTEIN METHODS AND COMPOSITIONS |
JP2001519538A (en) | 1997-10-10 | 2001-10-23 | プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ | Replica amplification of nucleic acid arrays |
US6548021B1 (en) * | 1997-10-10 | 2003-04-15 | President And Fellows Of Harvard College | Surface-bound, double-stranded DNA protein arrays |
US6511803B1 (en) | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
US6485944B1 (en) | 1997-10-10 | 2002-11-26 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
WO1999042621A2 (en) * | 1998-02-21 | 1999-08-26 | Tm Technologies, Inc. | Methods for identifying or characterising a site based on the thermodynamic properties of nucleic acids |
EP0945507A1 (en) * | 1998-03-27 | 1999-09-29 | Boehringer Mannheim Gmbh | Tumor specific expression controll region and its use |
US7321828B2 (en) * | 1998-04-13 | 2008-01-22 | Isis Pharmaceuticals, Inc. | System of components for preparing oligonucleotides |
US20040186071A1 (en) | 1998-04-13 | 2004-09-23 | Bennett C. Frank | Antisense modulation of CD40 expression |
CA2329130A1 (en) * | 1998-05-21 | 1999-11-25 | Isis Pharmaceuticals Inc. | Compositions and methods for non-parenteral delivery of oligonucleotides |
EP1080226A4 (en) * | 1998-05-21 | 2004-04-21 | Isis Pharmaceuticals Inc | Compositions and methods for topical delivery of oligonucleotides |
US6723517B1 (en) * | 1998-06-02 | 2004-04-20 | Minerva Biotechnologies Corporation | Use of self-assembled monolayers to probe the structure of a target molecule |
US6100035A (en) * | 1998-07-14 | 2000-08-08 | Cistem Molecular Corporation | Method of identifying cis acting nucleic acid elements |
US6203990B1 (en) | 1998-11-06 | 2001-03-20 | Mitokor | Method and system for pattern analysis, such as for analyzing oligonucleotide primer extension assay products |
DE69834208T2 (en) * | 1998-12-10 | 2007-01-25 | Daniel P. El Dorado Hills Cashman | BLOCKED POLYMERASE POLYNUCLEOTIDE IMMUNOASSAY AND KIT |
US7189506B1 (en) | 1999-03-03 | 2007-03-13 | Genelabs Technologies, Inc. | DNA binding compound-mediated molecular switch system |
US6824987B1 (en) * | 1999-05-11 | 2004-11-30 | President And Fellows Of Harvard College | Small molecule printing |
US7932213B2 (en) * | 1999-05-11 | 2011-04-26 | President And Fellows Of Harvard College | Small molecule printing |
DE19926216A1 (en) * | 1999-06-09 | 2001-02-22 | Metallgesellschaft Ag | Process for producing barium sulfate, barium sulfate and use of barium sulfate |
US6764858B2 (en) | 1999-09-29 | 2004-07-20 | Pharmacia & Upjohn Company | Methods for creating a compound library |
US6677160B1 (en) | 1999-09-29 | 2004-01-13 | Pharmacia & Upjohn Company | Methods for creating a compound library and identifying lead chemical templates and ligands for target molecules |
AU7539800A (en) * | 1999-10-01 | 2001-05-10 | Sangamo Biosciences, Inc. | Dna library |
US6613517B2 (en) | 2000-01-15 | 2003-09-02 | Genelabs Technologies, Inc. | Nucleic acid binding assay and selection method |
US6641820B1 (en) | 2000-01-19 | 2003-11-04 | Allergan, Inc. | Clostridial toxin derivatives and methods to treat pain |
US7138127B1 (en) | 2000-01-19 | 2006-11-21 | Allergan, Inc. | Clostridial toxin derivatives and methods for treating pain |
US6500436B2 (en) | 2000-01-19 | 2002-12-31 | Allergan, Inc. | Clostridial toxin derivatives and methods for treating pain |
AU2001251502A1 (en) * | 2000-04-17 | 2001-10-30 | Pharmacia And Upjohn Company | Nuclear magnetic resonance methods for identifying sites in papillomavirus e2 protein |
AU2001255748B2 (en) * | 2000-04-28 | 2006-08-10 | Sangamo Therapeutics, Inc. | Methods for binding an exogenous molecule to cellular chromatin |
DE60137722D1 (en) | 2000-06-13 | 2009-04-02 | Univ Boston | USE OF MASS-MATCHED NUCLEOTIDES IN THE ANALYSIS OF OLIGONUCLEOTIDE MIXTURES AND IN THE HIGH-MULTIPLEXIC NUCLEIC ACID SEQUENCING |
CN1330753C (en) * | 2000-08-30 | 2007-08-08 | 北卡罗莱纳州立大学 | Transgenic plants containing molecular decoys that alter protein content therein |
CN1258593C (en) * | 2000-11-07 | 2006-06-07 | 北卡罗莱纳州立大学 | Putrescine-N-methyl transferase promoter |
WO2002046769A2 (en) * | 2000-12-05 | 2002-06-13 | The Penn State Research Foundation | A monoclonal antibody-based diagnostic assay for gamma fibrinogen |
JP2004533228A (en) * | 2001-01-19 | 2004-11-04 | エジー バイオサイエンシーズ, インコーポレイテッド | Computer-based assembly of a polynucleotide encoding a target polypeptide |
WO2002086086A2 (en) * | 2001-04-25 | 2002-10-31 | Wisconsin Alumni Research Foundation | Methods to identify agents that bind the flap-tip helix of rna polymerase |
US6696256B1 (en) | 2001-06-08 | 2004-02-24 | Pandmics, Inc. | Method, array and kit for detecting activated transcription factors by hybridization array |
IL159213A0 (en) * | 2001-06-08 | 2004-06-01 | Vector Tobacco Ltd | Modifying nicotine and nitrosamine levels in tobacco |
US20060157072A1 (en) * | 2001-06-08 | 2006-07-20 | Anthony Albino | Method of reducing the harmful effects of orally or transdermally delivered nicotine |
US6924113B2 (en) | 2001-06-08 | 2005-08-02 | Panomics, Inc. | Method and kit for isolating DNA probes that bind to activated transcription factors |
US6821737B2 (en) | 2001-06-08 | 2004-11-23 | Panomics, Inc. | Method for screening for drug candidates for modulating transcription factor activity |
US7981842B2 (en) | 2001-06-08 | 2011-07-19 | Panomics, Inc. | Method for detecting transcription factor-protein interactions |
US20040214166A1 (en) * | 2001-06-08 | 2004-10-28 | Xianqiang Li | Method for identifying a disease state based on a detected mixture of activated transcription factors |
US7115232B2 (en) * | 2001-07-13 | 2006-10-03 | Hudson Gordon S | Fluorescence validation microplate and method of use |
WO2003051314A2 (en) * | 2001-12-18 | 2003-06-26 | Medallion Biomedical, Llc | Antibiotic compounds |
US7001718B2 (en) * | 2001-12-20 | 2006-02-21 | Massachusetts Institute Of Technology | Method of inhibiting pathogenicity of infectious agents |
US20030143547A1 (en) * | 2002-01-24 | 2003-07-31 | Xianqiang Li | Method for identifying multiple activated transcription factors |
EP1499188A4 (en) * | 2002-04-09 | 2007-11-14 | Vector Tobacco Ltd | Tobacco having reduced nicotine and nitrosamines |
DE60335000D1 (en) * | 2002-09-06 | 2010-12-30 | Isogenica Ltd | IN VITRO PEPTIDE EXPRESSION BANK |
US8084432B2 (en) * | 2003-02-13 | 2011-12-27 | Isis Pharmaceuticals, Inc. | Compositions and methods for treatment of pouchitis |
US7004001B2 (en) * | 2003-02-26 | 2006-02-28 | Formcek Cleveland, Inc. | Roll forming apparatus for forming sheet material into multiple shapes |
US7750142B2 (en) * | 2003-04-28 | 2010-07-06 | Isis Pharmaceuticals, Inc. | Modulation of glucagon receptor expression |
US7399853B2 (en) * | 2003-04-28 | 2008-07-15 | Isis Pharmaceuticals | Modulation of glucagon receptor expression |
JP3711988B2 (en) * | 2003-05-12 | 2005-11-02 | 株式会社日立製作所 | Fine particle array analysis system, fine particle array kit, and chemical analysis method |
WO2005018552A2 (en) * | 2003-08-12 | 2005-03-03 | University Of Utah Research Foundation | Heparin binding proteins: sensors for heparin detection |
US20050142581A1 (en) * | 2003-09-04 | 2005-06-30 | Griffey Richard H. | Microrna as ligands and target molecules |
CN101384757A (en) * | 2006-01-03 | 2009-03-11 | 哈佛大学校长及研究员协会 | Small molecule printing |
EP3357490A1 (en) | 2006-04-28 | 2018-08-08 | Children's Hospital Medical Center | Fusogenic properties of saposin c and related proteins and polypeptides for application to transmembrane drug delivery systems |
US8639445B2 (en) * | 2007-07-23 | 2014-01-28 | Microsoft Corporation | Identification of related residues in biomolecular sequences by multiple sequence alignment and phylogenetic analysis |
EP2214696A1 (en) * | 2007-10-23 | 2010-08-11 | Allergan, Inc. | Methods of treating chronic neurogenic inflammation using modified clostridial toxins |
USRE47320E1 (en) | 2007-11-20 | 2019-03-26 | Ionis Pharmaceuticals, Inc. | Modulation of CD40 expression |
US8889394B2 (en) * | 2009-09-07 | 2014-11-18 | Empire Technology Development Llc | Multiple domain proteins |
CA2824877A1 (en) | 2011-01-28 | 2012-08-02 | Biodesix, Inc. | Predictive test for selection of metastatic breast cancer patients for hormonal and combination therapy |
EP2758533B1 (en) | 2011-09-20 | 2018-04-11 | Ionis Pharmaceuticals, Inc. | Antisense modulation of gcgr expression |
DK2790736T3 (en) | 2011-12-12 | 2018-05-07 | Oncoimmunin Inc | In vivo delivery of oligonucleotides |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4270924A (en) * | 1979-04-10 | 1981-06-02 | Crooke Stanley T | Diagnostic method for detecting cancer |
US4257774A (en) * | 1979-07-16 | 1981-03-24 | Meloy Laboratories, Inc. | Intercalation inhibition assay for compounds that interact with DNA or RNA |
US4942227A (en) * | 1982-01-11 | 1990-07-17 | California Institute Of Technology | Bifunctional molecules having a DNA intercalator or DNA groove binder linked to ethylene diamine tetraacetic acid, their preparation and use to cleave DNA |
US4665184A (en) * | 1983-10-12 | 1987-05-12 | California Institute Of Technology | Bifunctional molecules having a DNA intercalator or DNA groove binder linked to ethylene diamine tetraacetic acid |
WO1987004170A1 (en) * | 1986-01-09 | 1987-07-16 | Massachusetts Institute Of Technology | Nuclear factors associated with transcriptional regulation |
US5071773A (en) * | 1986-10-24 | 1991-12-10 | The Salk Institute For Biological Studies | Hormone receptor-related bioassays |
US5096815A (en) * | 1989-01-06 | 1992-03-17 | Protein Engineering Corporation | Generation and selection of novel dna-binding proteins and polypeptides |
CA2092000A1 (en) * | 1990-09-24 | 1992-03-25 | Roger Brent | Screening assays |
WO1992013063A1 (en) * | 1991-01-18 | 1992-08-06 | Oncogene Science, Inc. | Methods of transcriptionally modulating expression of growth factor genes and growth factor receptor genes |
DK0593618T3 (en) * | 1991-06-27 | 1998-11-23 | Genelabs Tech Inc | Screening assay for detection of DNA-binding molecules |
US5726014A (en) * | 1991-06-27 | 1998-03-10 | Genelabs Technologies, Inc. | Screening assay for the detection of DNA-binding molecules |
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1992
- 1992-06-26 DK DK92915116T patent/DK0593618T3/en active
- 1992-06-26 CA CA002112130A patent/CA2112130C/en not_active Expired - Fee Related
- 1992-06-26 EP EP92915116A patent/EP0593618B1/en not_active Expired - Lifetime
- 1992-06-26 DE DE69225227T patent/DE69225227T2/en not_active Expired - Fee Related
- 1992-06-26 AT AT92915116T patent/ATE165397T1/en not_active IP Right Cessation
- 1992-06-26 KR KR1019930704049A patent/KR100235575B1/en not_active IP Right Cessation
- 1992-06-26 EP EP97114919A patent/EP0823486A3/en not_active Withdrawn
- 1992-06-26 WO PCT/US1992/005476 patent/WO1993000446A1/en active IP Right Grant
- 1992-06-26 ES ES92915116T patent/ES2117050T3/en not_active Expired - Lifetime
- 1992-06-26 JP JP50170593A patent/JP3169610B2/en not_active Expired - Fee Related
- 1992-06-26 AU AU22978/92A patent/AU655839B2/en not_active Ceased
- 1992-12-23 US US07/996,783 patent/US5693463A/en not_active Expired - Lifetime
-
1993
- 1993-06-22 US US08/081,070 patent/US5306619A/en not_active Expired - Lifetime
-
1995
- 1995-06-07 US US08/476,876 patent/US5744131A/en not_active Expired - Lifetime
- 1995-06-07 US US08/475,221 patent/US5738990A/en not_active Expired - Lifetime
- 1995-06-07 US US08/484,499 patent/US5716780A/en not_active Expired - Lifetime
-
1998
- 1998-09-15 HK HK98110639A patent/HK1010058A1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
ATE165397T1 (en) | 1998-05-15 |
EP0593618A1 (en) | 1994-04-27 |
JP3169610B2 (en) | 2001-05-28 |
WO1993000446A1 (en) | 1993-01-07 |
EP0823486A3 (en) | 2004-02-11 |
HK1010058A1 (en) | 1999-06-11 |
CA2112130C (en) | 1996-08-06 |
AU2297892A (en) | 1993-01-25 |
AU655839B2 (en) | 1995-01-12 |
DE69225227T2 (en) | 1998-10-29 |
EP0593618B1 (en) | 1998-04-22 |
JPH07500491A (en) | 1995-01-19 |
DE69225227D1 (en) | 1998-05-28 |
US5716780A (en) | 1998-02-10 |
EP0823486A2 (en) | 1998-02-11 |
US5744131A (en) | 1998-04-28 |
DK0593618T3 (en) | 1998-11-23 |
KR100235575B1 (en) | 1999-12-15 |
US5306619A (en) | 1994-04-26 |
US5738990A (en) | 1998-04-14 |
ES2117050T3 (en) | 1998-08-01 |
US5693463A (en) | 1997-12-02 |
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